RLG00000024914

Macrophage migration inhibitory

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0014219
Physical Location & Seq
Reverse (-)
40250412 .. 40252216
1805 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024914

Sequence Viewer

Length: 342 bp
ATGCCTTGCCTTTACCTCTCCACCAACGTGAACCTAGATGGAGTTGACATTAATCCTATCTTCTCCGAAGCTACGAAAGCCATCTCTGCCATTACCGGAAAGCCCAAAAGCCATGTGATGGTGTTACTCAAGGGCTCAGTTCCCATATCATTTGGTGCAAGCACTAGCGAACCGGCGGGGTACGGTGAGTTGGTGGCTTTGGGCGGCATTACGAAGGCGGTGAAGCGGGAGCTGATCGCTACGCTCGGCATAATTCTGCAGAACTCGAAGCTCGGCATCCCCAGGACTCGTTTTTTCCTTAAAGTTGTTGATGTAAGTACTCCTATGGGATCCAAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

114

Amino Acids

11.87

Weight (kDa)

9.61

Isoelectric Point (pI)

13.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 2 - 106 3.4e-07 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51660
fragaria_vesca FvH4_6g10610
malus_domestica MD04G1166700.v1.1 MD12G1179700.v1.1
prunus_persica Prupe.6G284900_v2.0.a1
pyrus_communis pycom04g14810 pycom12g16980
rosa_chinensis RchiOBHm_Chr3g0461611
rosa_laevigata RLG00000024914
rosa_multiflora Rmu_sc0003578.1_g000004
rosa_roxburghii Rroxscaffold_6G00418100 Rroxscaffold_6G00418470
rosa_rugosa Rorug03G0052400
rosa_samantha Rh3AG110500 Rh3CG115600 Rh3DG115500
rosa_wichuraiana Rw3G009350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 118
AciI CCGC 4 cut(s) 176, 204, 218, 226
AclWI GGATC 2 cut(s) 324, 337
AfaI GTAC 2 cut(s) 182, 319
AfiI CCNNNNNNNGG 1 cut(s) 118
AjnI CCWGG 1 cut(s) 281
AleI CACNNNNGTG 1 cut(s) 26
AluBI AGCT 3 cut(s) 71, 232, 271
AluI AGCT 3 cut(s) 71, 232, 271
AlwI GGATC 2 cut(s) 324, 337
AseI ATTAAT 1 cut(s) 51
AsuHPI GGTGA 2 cut(s) 197, 232
BamHI GGATCC 1 cut(s) 329
BanII GRGCYC 1 cut(s) 137
BccI CCATC 3 cut(s) 32, 89, 112
BciT130I CCWGG 1 cut(s) 283
BfaI CTAG 2 cut(s) 35, 165
BfmI CTRYAG 1 cut(s) 257
BisI GCNGC 1 cut(s) 205
BlsI GCNGC 1 cut(s) 206
BmcAI AGTACT 1 cut(s) 319
Bme1390I CCNGG 1 cut(s) 283
BmiI GGNNCC 1 cut(s) 331
BmrFI CCNGG 1 cut(s) 283
BmsI GCATC 1 cut(s) 285
BpuEI CTTGAG 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 281
BsaWI WCCGGW 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse118I RCCGGY 1 cut(s) 172
BseBI CCWGG 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 281
BseGI GGATG 1 cut(s) 276
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMII CTCAG 1 cut(s) 150
BsiSI CCGG 2 cut(s) 96, 173
BslI CCNNNNNNNGG 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 137
Bsp143I GATC 2 cut(s) 234, 329
BspACI CCGC 4 cut(s) 176, 204, 218, 226
BspCNI CTCAG 1 cut(s) 149
BspLI GGNNCC 1 cut(s) 331
BspMAI CTGCAG 1 cut(s) 261
BspPI GGATC 2 cut(s) 324, 337
BsrFI RCCGGY 1 cut(s) 172
BssAI RCCGGY 1 cut(s) 172
BssECI CCNNGG 1 cut(s) 281
BssMI GATC 2 cut(s) 234, 329
Bst2UI CCWGG 1 cut(s) 283
Bst4CI ACNGT 2 cut(s) 185, 339
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 1 cut(s) 136
BstF5I GGATG 1 cut(s) 276
BstKTI GATC 2 cut(s) 237, 332
BstMBI GATC 2 cut(s) 234, 329
BstMWI GCNNNNNNNGC 2 cut(s) 77, 86
BstNI CCWGG 1 cut(s) 283
BstSCI CCNGG 1 cut(s) 281
BstSFI CTRYAG 1 cut(s) 257
BstX2I RGATCY 1 cut(s) 329
BstYI RGATCY 1 cut(s) 329
BtsCI GGATG 1 cut(s) 276
Cac8I GCNNGC 1 cut(s) 160
Cfr10I RCCGGY 1 cut(s) 172
Csp6I GTAC 2 cut(s) 181, 318
CviAII CATG 1 cut(s) 113
CviJI RGCY 8 cut(s) 71, 80, 103, 111, 135, 197, 232, 271
CviKI_1 RGCY 8 cut(s) 71, 80, 103, 111, 135, 197, 232, 271
CviQI GTAC 2 cut(s) 181, 318
DdeI CTNAG 1 cut(s) 136
DpnI GATC 2 cut(s) 236, 331
DpnII GATC 2 cut(s) 234, 329
Eco24I GRGCYC 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 281
EcoT38I GRGCYC 1 cut(s) 137
FaeI CATG 1 cut(s) 116
FaiI YATR 4 cut(s) 114, 146, 251, 326
FatI CATG 1 cut(s) 112
FauI CCCGC 2 cut(s) 169, 219
Fnu4HI GCNGC 1 cut(s) 205
FokI GGATG 1 cut(s) 263
FriOI GRGCYC 1 cut(s) 137
Fsp4HI GCNGC 1 cut(s) 205
FspBI CTAG 2 cut(s) 35, 165
GluI GCNGC 1 cut(s) 205
HapII CCGG 2 cut(s) 96, 173
Hin1II CATG 1 cut(s) 116
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HinfI GANTC 1 cut(s) 286
HpaII CCGG 2 cut(s) 96, 173
HphI GGTGA 2 cut(s) 197, 232
Hpy166II GTNNAC 2 cut(s) 31, 46
Hpy188I TCNGA 1 cut(s) 67
Hpy8I GTNNAC 2 cut(s) 31, 46
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 2 cut(s) 185, 339
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 2 cut(s) 158, 259
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 86
HpyF3I CTNAG 1 cut(s) 136
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 1 cut(s) 116
Kzo9I GATC 2 cut(s) 234, 329
LmnI GCTCC 1 cut(s) 229
LpnPI CCDG 4 cut(s) 109, 186, 268, 295
LweI GCATC 1 cut(s) 285
MaeI CTAG 2 cut(s) 35, 165
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 123
MalI GATC 2 cut(s) 236, 331
MboI GATC 2 cut(s) 234, 329
MboII GAAGA 1 cut(s) 52
MflI RGATCY 1 cut(s) 329
MhlI GDGCHC 1 cut(s) 137
MluCI AATT 1 cut(s) 252
MlyI GAGTC 1 cut(s) 280
MnlI CCTC 1 cut(s) 26
MseI TTAA 2 cut(s) 51, 300
MslI CAYNNNNRTG 1 cut(s) 26
MspI CCGG 2 cut(s) 96, 173
MspR9I CCNGG 1 cut(s) 283
MvaI CCWGG 1 cut(s) 283
MwoI GCNNNNNNNGC 2 cut(s) 77, 86
NdeII GATC 2 cut(s) 234, 329
NlaIII CATG 1 cut(s) 116
NlaIV GGNNCC 1 cut(s) 331
NmeAIII GCCGAG 2 cut(s) 225, 252
OliI CACNNNNGTG 1 cut(s) 26
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 1 cut(s) 206
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
PshBI ATTAAT 1 cut(s) 51
Psp6I CCWGG 1 cut(s) 281
PspGI CCWGG 1 cut(s) 281
PspN4I GGNNCC 1 cut(s) 331
PstI CTGCAG 1 cut(s) 261
PsuI RGATCY 1 cut(s) 329
RsaI GTAC 2 cut(s) 182, 319
RsaNI GTAC 2 cut(s) 181, 318
RseI CAYNNNNRTG 1 cut(s) 26
SaqAI TTAA 2 cut(s) 51, 300
SatI GCNGC 1 cut(s) 205
Sau3AI GATC 2 cut(s) 234, 329
ScaI AGTACT 1 cut(s) 319
SchI GAGTC 1 cut(s) 280
ScrFI CCNGG 1 cut(s) 283
SduI GDGCHC 1 cut(s) 137
SetI ASST 6 cut(s) 18, 30, 36, 73, 234, 273
SfaNI GCATC 1 cut(s) 285
SfcI CTRYAG 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 1 cut(s) 252
SsiI CCGC 4 cut(s) 176, 204, 218, 226
SspMI CTAG 2 cut(s) 35, 165
StyD4I CCNGG 1 cut(s) 281
TaaI ACNGT 2 cut(s) 185, 339
TaiI ACGT 1 cut(s) 30
TaqI TCGA 1 cut(s) 266
TasI AATT 1 cut(s) 252
TatI WGTACW 1 cut(s) 317
TauI GCSGC 1 cut(s) 207
Tru1I TTAA 2 cut(s) 51, 300
Tru9I TTAA 2 cut(s) 51, 300
Van91I CCANNNNNTGG 1 cut(s) 118
VspI ATTAAT 1 cut(s) 51
XspI CTAG 2 cut(s) 35, 165
ZrmI AGTACT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.