MD04G1166700.v1.1

Macrophage migration inhibitory

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014219
Physical Location & Seq
Reverse (-)
25691824 .. 25693648
1825 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1166700.v1.1.491

Sequence Viewer

Length: 339 bp
ATGCCTTGCCTTTATATCTCCACCAACGTCAACCTTGACGGAGTCGACGCTGATTCCATCTTTTCCGAAGCCACCAAAGCCATCTCTGCCATTACTGGAAAGCCCGAAAATTTTGTGATGGTGGTACTTAAGGGATCGGTGCCCATATCGTTCGGGAAGAGTACCACTATACCGGCATCATATGGCGAGTTAGTAGCAATGGGTGGCATTACCACGACCGTGAAGAGGCAGCTGATCGCCACTCTCGGCACGATTTTTGAGTCCAAGCTTTCGATCCCAAAAACCCGATTTTTTCTTAAAGTTGTTGATTTAAGTACTCCTACAGGATCTAAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

113

Amino Acids

11.84

Weight (kDa)

9.17

Isoelectric Point (pI)

12.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 2 - 106 2.9e-10 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51660
fragaria_vesca FvH4_6g10610
malus_domestica MD04G1166700.v1.1 MD12G1179700.v1.1
prunus_persica Prupe.6G284900_v2.0.a1
pyrus_communis pycom04g14810 pycom12g16980
rosa_chinensis RchiOBHm_Chr3g0461611
rosa_laevigata RLG00000024914
rosa_multiflora Rmu_sc0003578.1_g000004
rosa_roxburghii Rroxscaffold_6G00418100 Rroxscaffold_6G00418470
rosa_rugosa Rorug03G0052400
rosa_samantha Rh3AG110500 Rh3CG115600 Rh3DG115500
rosa_wichuraiana Rw3G009350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 139
AccI GTMKAC 1 cut(s) 45
AclWI GGATC 3 cut(s) 142, 268, 334
AcsI RAATTY 1 cut(s) 109
AfaI GTAC 3 cut(s) 126, 163, 316
AfiI CCNNNNNNNGG 1 cut(s) 225
AflII CTTAAG 1 cut(s) 128
AleI CACNNNNGTG 1 cut(s) 218
AluBI AGCT 2 cut(s) 232, 268
AluI AGCT 2 cut(s) 232, 268
AlwI GGATC 3 cut(s) 142, 268, 334
ApeKI GCWGC 1 cut(s) 229
ApoI RAATTY 1 cut(s) 109
BaeGI GKGCMC 1 cut(s) 144
BanI GGYRCC 1 cut(s) 139
BbvI GCAGC 1 cut(s) 241
BccI CCATC 3 cut(s) 65, 89, 112
BfmI CTRYAG 1 cut(s) 321
BfrI CTTAAG 1 cut(s) 128
BisI GCNGC 1 cut(s) 230
BlsI GCNGC 1 cut(s) 231
BmcAI AGTACT 1 cut(s) 316
BmiI GGNNCC 1 cut(s) 141
BmsI GCATC 1 cut(s) 185
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse118I RCCGGY 1 cut(s) 172
Bse1I ACTGG 1 cut(s) 100
Bse3DI GCAATG 1 cut(s) 204
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMI GCAATG 1 cut(s) 204
BseNI ACTGG 1 cut(s) 100
BseSI GKGCMC 1 cut(s) 144
BseXI GCAGC 1 cut(s) 241
Bsh1285I CGRYCG 1 cut(s) 219
BshNI GGYRCC 1 cut(s) 139
BsiEI CGRYCG 1 cut(s) 219
BsiSI CCGG 1 cut(s) 173
BslI CCNNNNNNNGG 1 cut(s) 225
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 4 cut(s) 134, 234, 273, 326
BspLI GGNNCC 1 cut(s) 141
BspPI GGATC 3 cut(s) 142, 268, 334
BspT107I GGYRCC 1 cut(s) 139
BspTI CTTAAG 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 204
BsrFI RCCGGY 1 cut(s) 172
BsrI ACTGG 1 cut(s) 100
BssAI RCCGGY 1 cut(s) 172
BssMI GATC 4 cut(s) 134, 234, 273, 326
Bst4CI ACNGT 2 cut(s) 220, 336
Bst6I CTCTTC 2 cut(s) 152, 218
BstAFI CTTAAG 1 cut(s) 128
BstKTI GATC 4 cut(s) 137, 237, 276, 329
BstMBI GATC 4 cut(s) 134, 234, 273, 326
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 2 cut(s) 77, 86
BstSFI CTRYAG 1 cut(s) 321
BstSLI GKGCMC 1 cut(s) 144
BstV1I GCAGC 1 cut(s) 241
BstX2I RGATCY 1 cut(s) 326
BstYI RGATCY 1 cut(s) 326
Cfr10I RCCGGY 1 cut(s) 172
CseI GACGC 1 cut(s) 56
Csp6I GTAC 3 cut(s) 125, 162, 315
CviJI RGCY 5 cut(s) 71, 80, 103, 232, 268
CviKI_1 RGCY 5 cut(s) 71, 80, 103, 232, 268
CviQI GTAC 3 cut(s) 125, 162, 315
DpnI GATC 4 cut(s) 136, 236, 275, 328
DpnII GATC 4 cut(s) 134, 234, 273, 326
Eam1104I CTCTTC 2 cut(s) 152, 218
EarI CTCTTC 2 cut(s) 152, 218
FaiI YATR 5 cut(s) 15, 146, 170, 181, 183
FauNDI CATATG 1 cut(s) 181
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 1 cut(s) 230
Fsp4HI GCNGC 1 cut(s) 230
GluI GCNGC 1 cut(s) 230
HapII CCGG 1 cut(s) 173
HgaI GACGC 1 cut(s) 56
HincII GTYRAC 2 cut(s) 31, 46
HindII GTYRAC 2 cut(s) 31, 46
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 3 cut(s) 42, 53, 260
HpaII CCGG 1 cut(s) 173
Hpy166II GTNNAC 2 cut(s) 31, 46
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 1 cut(s) 154
Hpy8I GTNNAC 2 cut(s) 31, 46
Hpy99I CGWCG 1 cut(s) 50
HpyCH4III ACNGT 2 cut(s) 220, 336
HpyCH4IV ACGT 1 cut(s) 27
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 86
HpySE526I ACGT 1 cut(s) 27
Kzo9I GATC 4 cut(s) 134, 234, 273, 326
LpnPI CCDG 3 cut(s) 81, 186, 309
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 1 cut(s) 185
MaeII ACGT 1 cut(s) 27
MalI GATC 4 cut(s) 136, 236, 275, 328
MboI GATC 4 cut(s) 134, 234, 273, 326
MboII GAAGA 2 cut(s) 169, 235
MflI RGATCY 1 cut(s) 326
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 1 cut(s) 109
MlyI GAGTC 2 cut(s) 51, 269
MnlI CCTC 1 cut(s) 219
MseI TTAA 3 cut(s) 129, 297, 311
MslI CAYNNNNRTG 1 cut(s) 218
MspA1I CMGCKG 1 cut(s) 232
MspCI CTTAAG 1 cut(s) 128
MspI CCGG 1 cut(s) 173
MwoI GCNNNNNNNGC 2 cut(s) 77, 86
NdeI CATATG 1 cut(s) 181
NdeII GATC 4 cut(s) 134, 234, 273, 326
NlaIV GGNNCC 1 cut(s) 141
NmeAIII GCCGAG 1 cut(s) 225
OliI CACNNNNGTG 1 cut(s) 218
PfeI GAWTC 1 cut(s) 53
PflFI GACNNNGTC 1 cut(s) 41
PkrI GCNGC 1 cut(s) 231
PleI GAGTC 2 cut(s) 50, 268
PpsI GAGTC 2 cut(s) 50, 268
PspN4I GGNNCC 1 cut(s) 141
PsuI RGATCY 1 cut(s) 326
PsyI GACNNNGTC 1 cut(s) 41
PvuII CAGCTG 1 cut(s) 232
RsaI GTAC 3 cut(s) 126, 163, 316
RsaNI GTAC 3 cut(s) 125, 162, 315
RseI CAYNNNNRTG 1 cut(s) 218
SalI GTCGAC 1 cut(s) 44
SaqAI TTAA 3 cut(s) 129, 297, 311
SatI GCNGC 1 cut(s) 230
Sau3AI GATC 4 cut(s) 134, 234, 273, 326
ScaI AGTACT 1 cut(s) 316
SchI GAGTC 2 cut(s) 51, 269
SduI GDGCHC 1 cut(s) 144
SetI ASST 4 cut(s) 30, 36, 234, 270
SfaNI GCATC 1 cut(s) 185
SfcI CTRYAG 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 1 cut(s) 109
TaaI ACNGT 2 cut(s) 220, 336
TaiI ACGT 1 cut(s) 30
TaqI TCGA 2 cut(s) 45, 272
TasI AATT 1 cut(s) 109
TatI WGTACW 1 cut(s) 314
TfiI GAWTC 1 cut(s) 53
Tru1I TTAA 3 cut(s) 129, 297, 311
Tru9I TTAA 3 cut(s) 129, 297, 311
TseI GCWGC 1 cut(s) 229
TspGWI ACGGA 1 cut(s) 54
Tth111I GACNNNGTC 1 cut(s) 41
Vha464I CTTAAG 1 cut(s) 128
XapI RAATTY 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 45
ZrmI AGTACT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.