FvH4_6g10610

Macrophage migration inhibitory

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014219
Physical Location & Seq
Forward (+)
6372555 .. 6374897
2343 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10610.t1

Sequence Viewer

Length: 342 bp
ATGCCTTGCCTTTACCTCTCCACCAACGTGAACCTGGATGGAGTGGACATTAATCCCATCTTCTCCGAAGCCACGAAAGCCATCTCTGCCATTACTGGGAAACCCGACACCCATATCATGGTGTTACTCAAGGGATCAGTTCCCATATCATTTGGTGCGAGCACTAGTGAACCGGCGGCGTACGGTGAGCTAGTGGCGTTGGGCGGCATTACAAAGGCGGTGAAGCGGGAGCTGATCGCTACACTCGGCACGATTCTGCAGAACTCGAAGCTCAGCGTCCCAAGGACTCGATTTTTCCTTAAAGTTGTTGATGTAAGTACTCCTATGGGATCCAAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

114

Amino Acids

11.9

Weight (kDa)

9.21

Isoelectric Point (pI)

19.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 2 - 106 4.1e-08 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51660
fragaria_vesca FvH4_6g10610
malus_domestica MD04G1166700.v1.1 MD12G1179700.v1.1
prunus_persica Prupe.6G284900_v2.0.a1
pyrus_communis pycom04g14810 pycom12g16980
rosa_chinensis RchiOBHm_Chr3g0461611
rosa_laevigata RLG00000024914
rosa_multiflora Rmu_sc0003578.1_g000004
rosa_roxburghii Rroxscaffold_6G00418100 Rroxscaffold_6G00418470
rosa_rugosa Rorug03G0052400
rosa_samantha Rh3AG110500 Rh3CG115600 Rh3DG115500
rosa_wichuraiana Rw3G009350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 118
AciI CCGC 4 cut(s) 176, 204, 218, 226
AclWI GGATC 3 cut(s) 142, 324, 337
AfaI GTAC 2 cut(s) 182, 319
AfiI CCNNNNNNNGG 2 cut(s) 96, 118
AhlI ACTAGT 1 cut(s) 164
AjnI CCWGG 1 cut(s) 33
AleI CACNNNNGTG 1 cut(s) 26
AluBI AGCT 3 cut(s) 190, 232, 271
AluI AGCT 3 cut(s) 190, 232, 271
Alw21I GWGCWC 1 cut(s) 164
AlwI GGATC 3 cut(s) 142, 324, 337
AseI ATTAAT 1 cut(s) 51
AsuHPI GGTGA 2 cut(s) 197, 232
BamHI GGATCC 1 cut(s) 329
Bbv12I GWGCWC 1 cut(s) 164
BccI CCATC 3 cut(s) 32, 65, 89
BciT130I CCWGG 1 cut(s) 35
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 2 cut(s) 165, 191
BfmI CTRYAG 1 cut(s) 257
BisI GCNGC 2 cut(s) 177, 205
BlpI GCTNAGC 1 cut(s) 272
BlsI GCNGC 2 cut(s) 178, 206
BmcAI AGTACT 1 cut(s) 319
Bme1390I CCNGG 1 cut(s) 35
BmiI GGNNCC 1 cut(s) 331
BmrFI CCNGG 1 cut(s) 35
BmrI ACTGGG 1 cut(s) 105
BmuI ACTGGG 1 cut(s) 105
Bpu1102I GCTNAGC 1 cut(s) 272
BpuEI CTTGAG 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 281
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 118
Bse118I RCCGGY 1 cut(s) 172
Bse1I ACTGG 1 cut(s) 100
BseBI CCWGG 1 cut(s) 35
BseDI CCNNGG 1 cut(s) 281
BseGI GGATG 1 cut(s) 43
BseLI CCNNNNNNNGG 2 cut(s) 96, 118
BseMII CTCAG 1 cut(s) 286
BseNI ACTGG 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 164
BsiSI CCGG 1 cut(s) 173
BsiWI CGTACG 1 cut(s) 180
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 2 cut(s) 96, 118
BsmFI GGGAC 1 cut(s) 263
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 3 cut(s) 134, 234, 329
Bsp1720I GCTNAGC 1 cut(s) 272
BspACI CCGC 4 cut(s) 176, 204, 218, 226
BspCNI CTCAG 1 cut(s) 285
BspLI GGNNCC 1 cut(s) 331
BspMAI CTGCAG 1 cut(s) 261
BspPI GGATC 3 cut(s) 142, 324, 337
BsrFI RCCGGY 1 cut(s) 172
BsrI ACTGG 1 cut(s) 100
BssAI RCCGGY 1 cut(s) 172
BssECI CCNNGG 1 cut(s) 281
BssMI GATC 3 cut(s) 134, 234, 329
BssT1I CCWWGG 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 35
Bst4CI ACNGT 2 cut(s) 185, 339
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 1 cut(s) 272
BstF5I GGATG 1 cut(s) 43
BstKTI GATC 3 cut(s) 137, 237, 332
BstMBI GATC 3 cut(s) 134, 234, 329
BstMWI GCNNNNNNNGC 2 cut(s) 77, 86
BstNI CCWGG 1 cut(s) 35
BstSCI CCNGG 1 cut(s) 33
BstSFI CTRYAG 1 cut(s) 257
BstX2I RGATCY 1 cut(s) 329
BstYI RGATCY 1 cut(s) 329
BtsCI GGATG 1 cut(s) 43
Cac8I GCNNGC 1 cut(s) 160
Cfr10I RCCGGY 1 cut(s) 172
CseI GACGC 1 cut(s) 265
Csp6I GTAC 2 cut(s) 181, 318
CviAII CATG 1 cut(s) 118
CviJI RGCY 5 cut(s) 71, 80, 190, 232, 271
CviKI_1 RGCY 5 cut(s) 71, 80, 190, 232, 271
CviQI GTAC 2 cut(s) 181, 318
DdeI CTNAG 1 cut(s) 272
DpnI GATC 3 cut(s) 136, 236, 331
DpnII GATC 3 cut(s) 134, 234, 329
Eco130I CCWWGG 1 cut(s) 281
EcoRII CCWGG 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 281
ErhI CCWWGG 1 cut(s) 281
FaeI CATG 1 cut(s) 121
FaiI YATR 4 cut(s) 114, 119, 146, 326
FaqI GGGAC 1 cut(s) 263
FatI CATG 1 cut(s) 117
FauI CCCGC 1 cut(s) 219
Fnu4HI GCNGC 2 cut(s) 177, 205
FokI GGATG 1 cut(s) 50
Fsp4HI GCNGC 2 cut(s) 177, 205
FspBI CTAG 2 cut(s) 165, 191
GluI GCNGC 2 cut(s) 177, 205
HapII CCGG 1 cut(s) 173
HgaI GACGC 1 cut(s) 265
Hin1II CATG 1 cut(s) 121
HinfI GANTC 2 cut(s) 253, 286
HpaII CCGG 1 cut(s) 173
HphI GGTGA 2 cut(s) 197, 232
Hpy166II GTNNAC 3 cut(s) 31, 46, 170
Hpy188I TCNGA 1 cut(s) 67
Hpy8I GTNNAC 3 cut(s) 31, 46, 170
HpyCH4III ACNGT 2 cut(s) 185, 339
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 1 cut(s) 259
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 86
HpyF3I CTNAG 1 cut(s) 272
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 1 cut(s) 121
Kzo9I GATC 3 cut(s) 134, 234, 329
LmnI GCTCC 1 cut(s) 229
LpnPI CCDG 4 cut(s) 20, 47, 81, 186
MaeI CTAG 2 cut(s) 165, 191
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 123
MalI GATC 3 cut(s) 136, 236, 331
MboI GATC 3 cut(s) 134, 234, 329
MboII GAAGA 1 cut(s) 52
MflI RGATCY 1 cut(s) 329
MhlI GDGCHC 1 cut(s) 164
MlyI GAGTC 1 cut(s) 280
MnlI CCTC 1 cut(s) 26
MseI TTAA 2 cut(s) 51, 300
MslI CAYNNNNRTG 1 cut(s) 26
MspI CCGG 1 cut(s) 173
MspR9I CCNGG 1 cut(s) 35
MvaI CCWGG 1 cut(s) 35
MwoI GCNNNNNNNGC 2 cut(s) 77, 86
NdeII GATC 3 cut(s) 134, 234, 329
NlaIII CATG 1 cut(s) 121
NlaIV GGNNCC 1 cut(s) 331
NmeAIII GCCGAG 1 cut(s) 225
OliI CACNNNNGTG 1 cut(s) 26
PfeI GAWTC 1 cut(s) 253
Pfl23II CGTACG 1 cut(s) 180
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 2 cut(s) 178, 206
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
PshBI ATTAAT 1 cut(s) 51
Psp6I CCWGG 1 cut(s) 33
PspGI CCWGG 1 cut(s) 33
PspLI CGTACG 1 cut(s) 180
PspN4I GGNNCC 1 cut(s) 331
PstI CTGCAG 1 cut(s) 261
PsuI RGATCY 1 cut(s) 329
RsaI GTAC 2 cut(s) 182, 319
RsaNI GTAC 2 cut(s) 181, 318
RseI CAYNNNNRTG 1 cut(s) 26
SaqAI TTAA 2 cut(s) 51, 300
SatI GCNGC 2 cut(s) 177, 205
Sau3AI GATC 3 cut(s) 134, 234, 329
ScaI AGTACT 1 cut(s) 319
SchI GAGTC 1 cut(s) 280
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 1 cut(s) 164
SetI ASST 6 cut(s) 18, 30, 36, 192, 234, 273
SfcI CTRYAG 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
SpeI ACTAGT 1 cut(s) 164
SsiI CCGC 4 cut(s) 176, 204, 218, 226
SspMI CTAG 2 cut(s) 165, 191
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 1 cut(s) 281
TaaI ACNGT 2 cut(s) 185, 339
TaiI ACGT 1 cut(s) 30
TaqI TCGA 2 cut(s) 266, 289
TatI WGTACW 1 cut(s) 317
TauI GCSGC 2 cut(s) 179, 207
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 2 cut(s) 51, 300
Tru9I TTAA 2 cut(s) 51, 300
Van91I CCANNNNNTGG 1 cut(s) 118
VspI ATTAAT 1 cut(s) 51
XcmI CCANNNNNNNNNTGG 1 cut(s) 31
XspI CTAG 2 cut(s) 165, 191
ZrmI AGTACT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.