AT3G52720

alpha carbonic anhydrase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010395
Physical Location & Seq
Reverse (-)
19538540 .. 19542286
3747 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G52720.1

Sequence Viewer

Length: 855 bp
ATGAAGATTATGATGATGATTAAGCTCTGCTTCTTCTCCATGTCCCTCATCTGCATTGCACCTGCAGATGCTCAGACAGAAGGAGTAGTGTTTGGATATAAAGGCAAAAATGGACCAAACCAATGGGGACACTTAAACCCTCACTTCACCACATGCGCGGTCGGTAAATTGCAATCTCCAATTGATATTCAAAGGAGGCAAATATTTTACAACCACAAATTGAATTCAATACACCGTGAATACTACTTCACAAACGCAACACTAGTGAACCACGTCTGTAATGTTGCCATGTTCTTCGGGGAGGGAGCAGGAGATGTGATAATAGAAAACAAGAACTATACCTTACTGCAAATGCATTGGCACACTCCTTCTGAACATCACCTCCATGGAGTCCAATATGCAGCTGAGCTGCACATGGTACACCAAGCAAAAGATGGAAGCTTTGCTGTGGTGGCAAGTCTCTTCAAAATCGGCACTGAAGAGCCTTTCCTCTCTCAGATGAAGGAGAAATTGGTGAAGCTAAAGGAAGAGAGACTCAAAGGGAACCACACAGCACAAGTGGAAGTAGGAAGAATCGACACAAGACACATTGAACGTAAGACTCGAAAGTACTACAGATACATTGGTTCACTCACTACTCCTCCTTGCTCCGAGAACGTTTCTTGGACCATCCTTGGCAAGGTGAGGTCAATGTCAAAGGAACAAGTAGAACTACTCAGATCTCCATTGGACACTTCTTTCAAGAACAATTCAAGACCGTGTCAACCCCTCAACGGCCGGAGAGTTGAGATGTTCCACGACCACGAGCGTGTCGATAAAAAAGAAACCGGTAACAAAAAGAAAAAACCCAATTAA

Protein Analysis

284

Amino Acids

32.7

Weight (kDa)

9.45

Isoelectric Point (pI)

37.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 37 - 263 5.9e-40 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52720 AT3G52720 AT3G52720 AT3G52720
fragaria_vesca FvH4_6g18830 FvH4_6g18830
malus_domestica MD12G1024600.v1.1
prunus_persica Prupe.7G109300_v2.0.a1 Prupe.7G109300_v2.0.a1
pyrus_communis pycom12g02430
rosa_chinensis RchiOBHm_Chr3g0473841
rosa_laevigata RLG00000023979
rosa_multiflora Rmu_sc0005561.1_g000003
rosa_roxburghii Rroxscaffold_6G00407730
rosa_rugosa Rorug03G0135900 Rorug03G0136000
rosa_samantha Rh3AG186400 Rh3BG215000 Rh3CG211700 Rh3DG210800
rosa_wichuraiana Rw3G017050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 70
Acc36I ACCTGC 1 cut(s) 70
AccII CGCG 1 cut(s) 158
AciI CCGC 1 cut(s) 158
AclI AACGTT 1 cut(s) 657
AcoI YGGCCR 1 cut(s) 775
AcsI RAATTY 1 cut(s) 223
AcuI CTGAAG 1 cut(s) 498
AfaI GTAC 2 cut(s) 420, 611
AfiI CCNNNNNNNGG 2 cut(s) 679, 773
AgeI ACCGGT 1 cut(s) 827
AgsI TTSAA 7 cut(s) 191, 223, 228, 466, 593, 742, 753
AhlI ACTAGT 1 cut(s) 262
AjiI CACGTC 1 cut(s) 274
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AleI CACNNNNGTG 1 cut(s) 807
AloI GAACNNNNNNTCC 2 cut(s) 366, 398
AluBI AGCT 5 cut(s) 25, 404, 409, 441, 520
AluI AGCT 5 cut(s) 25, 404, 409, 441, 520
Alw26I GTCTC 2 cut(s) 464, 526
AoxI GGCC 1 cut(s) 775
ApeKI GCWGC 2 cut(s) 401, 409
ApoI RAATTY 1 cut(s) 223
AsiGI ACCGGT 1 cut(s) 827
AspLEI GCGC 1 cut(s) 158
AspS9I GGNCC 2 cut(s) 113, 666
AsuHPI GGTGA 4 cut(s) 139, 371, 526, 694
AvaII GGWCC 2 cut(s) 113, 666
BaeI ACNNNNGTAYC 2 cut(s) 610, 643
BauI CACGAG 1 cut(s) 803
BbvI GCAGC 2 cut(s) 396, 413
BccI CCATC 2 cut(s) 428, 677
BceAI ACGGC 1 cut(s) 790
BcoDI GTCTC 2 cut(s) 464, 526
BcuI ACTAGT 1 cut(s) 262
BfaI CTAG 1 cut(s) 263
BfmI CTRYAG 2 cut(s) 63, 613
BfuAI ACCTGC 1 cut(s) 70
BglII AGATCT 1 cut(s) 719
BisI GCNGC 2 cut(s) 402, 410
BlpI GCTNAGC 1 cut(s) 405
BlsI GCNGC 2 cut(s) 403, 411
BmcAI AGTACT 1 cut(s) 611
Bme18I GGWCC 2 cut(s) 113, 666
BmgBI CACGTC 1 cut(s) 274
BmgT120I GGNCC 2 cut(s) 113, 666
BmiI GGNNCC 1 cut(s) 545
BmsI GCATC 1 cut(s) 58
Bpu1102I GCTNAGC 1 cut(s) 405
BsaJI CCNNGG 2 cut(s) 385, 673
BsaWI WCCGGW 1 cut(s) 827
BsaXI ACNNNNNCTCC 4 cut(s) 366, 396, 625, 655
Bsc4I CCNNNNNNNGG 2 cut(s) 679, 773
Bse118I RCCGGY 1 cut(s) 827
Bse3DI GCAATG 1 cut(s) 54
BseDI CCNNGG 2 cut(s) 385, 673
BseGI GGATG 1 cut(s) 669
BseLI CCNNNNNNNGG 2 cut(s) 679, 773
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 4 cut(s) 86, 396, 509, 730
BseRI GAGGAG 1 cut(s) 630
BseX3I CGGCCG 1 cut(s) 775
BseXI GCAGC 2 cut(s) 396, 413
BsgI GTGCAG 1 cut(s) 395
Bsh1236I CGCG 1 cut(s) 158
Bsh1285I CGRYCG 2 cut(s) 162, 778
BshFI GGCC 1 cut(s) 777
BshTI ACCGGT 1 cut(s) 827
BsiEI CGRYCG 2 cut(s) 162, 778
BsiSI CCGG 2 cut(s) 778, 828
BslFI GGGAC 2 cut(s) 28, 141
BslI CCNNNNNNNGG 2 cut(s) 679, 773
BsmAI GTCTC 2 cut(s) 464, 526
BsmFI GGGAC 2 cut(s) 28, 141
BsnI GGCC 1 cut(s) 777
Bsp143I GATC 1 cut(s) 719
Bsp1720I GCTNAGC 1 cut(s) 405
Bsp19I CCATGG 1 cut(s) 385
BspACI CCGC 1 cut(s) 158
BspANI GGCC 1 cut(s) 777
BspCNI CTCAG 4 cut(s) 85, 397, 508, 729
BspFNI CGCG 1 cut(s) 158
BspLI GGNNCC 1 cut(s) 545
BspMAI CTGCAG 1 cut(s) 67
BspMI ACCTGC 1 cut(s) 70
BspQI GCTCTTC 1 cut(s) 474
BsrDI GCAATG 1 cut(s) 54
BsrFI RCCGGY 1 cut(s) 827
BssAI RCCGGY 1 cut(s) 827
BssECI CCNNGG 2 cut(s) 385, 673
BssMI GATC 1 cut(s) 719
BssSI CACGAG 1 cut(s) 803
BssT1I CCWWGG 2 cut(s) 385, 673
Bst2BI CACGAG 1 cut(s) 803
Bst4CI ACNGT 2 cut(s) 236, 759
Bst6I CTCTTC 3 cut(s) 467, 474, 522
BstDEI CTNAG 4 cut(s) 72, 405, 495, 716
BstDSI CCRYGG 1 cut(s) 385
BstENI CCTNNNNNAGG 1 cut(s) 677
BstF5I GGATG 1 cut(s) 669
BstFNI CGCG 1 cut(s) 158
BstHHI GCGC 1 cut(s) 158
BstKTI GATC 1 cut(s) 722
BstMAI GTCTC 2 cut(s) 464, 526
BstMBI GATC 1 cut(s) 719
BstMCI CGRYCG 2 cut(s) 162, 778
BstMWI GCNNNNNNNGC 1 cut(s) 452
BstNSI RCATGY 1 cut(s) 156
BstSFI CTRYAG 2 cut(s) 63, 613
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 2 cut(s) 396, 413
BstX2I RGATCY 1 cut(s) 719
BstXI CCANNNNNNTGG 1 cut(s) 123
BstYI RGATCY 1 cut(s) 719
BstZI CGGCCG 1 cut(s) 775
BsuRI GGCC 1 cut(s) 777
BtgI CCRYGG 1 cut(s) 385
BtrI CACGTC 1 cut(s) 274
BtsCI GGATG 1 cut(s) 669
BtsIMutI CAGTG 1 cut(s) 474
BveI ACCTGC 1 cut(s) 70
CfoI GCGC 1 cut(s) 158
Cfr10I RCCGGY 1 cut(s) 827
Cfr13I GGNCC 2 cut(s) 113, 666
Csp6I GTAC 2 cut(s) 419, 610
CspAI ACCGGT 1 cut(s) 827
CviAII CATG 5 cut(s) 40, 153, 289, 386, 415
CviJI RGCY 7 cut(s) 25, 404, 409, 441, 484, 520, 777
CviKI_1 RGCY 7 cut(s) 25, 404, 409, 441, 484, 520, 777
CviQI GTAC 2 cut(s) 419, 610
DdeI CTNAG 4 cut(s) 72, 405, 495, 716
DpnI GATC 1 cut(s) 721
DpnII GATC 1 cut(s) 719
EaeI YGGCCR 1 cut(s) 775
EagI CGGCCG 1 cut(s) 775
Eam1104I CTCTTC 3 cut(s) 467, 474, 522
EarI CTCTTC 3 cut(s) 467, 474, 522
EclXI CGGCCG 1 cut(s) 775
Eco130I CCWWGG 2 cut(s) 385, 673
Eco47I GGWCC 2 cut(s) 113, 666
Eco52I CGGCCG 1 cut(s) 775
Eco57I CTGAAG 1 cut(s) 498
EcoNI CCTNNNNNAGG 1 cut(s) 677
EcoRI GAATTC 1 cut(s) 223
EcoT14I CCWWGG 2 cut(s) 385, 673
EcoT22I ATGCAT 1 cut(s) 357
ErhI CCWWGG 2 cut(s) 385, 673
FaeI CATG 5 cut(s) 43, 156, 292, 389, 418
FaiI YATR 9 cut(s) 11, 41, 99, 154, 290, 339, 387, 399, 416
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FaqI GGGAC 2 cut(s) 28, 141
FatI CATG 5 cut(s) 39, 152, 288, 385, 414
Fnu4HI GCNGC 2 cut(s) 402, 410
FokI GGATG 1 cut(s) 656
Fsp4HI GCNGC 2 cut(s) 402, 410
FspBI CTAG 1 cut(s) 263
GlaI GCGC 1 cut(s) 157
GluI GCNGC 2 cut(s) 402, 410
HaeIII GGCC 1 cut(s) 777
HapII CCGG 2 cut(s) 778, 828
HhaI GCGC 1 cut(s) 158
Hin1II CATG 5 cut(s) 43, 156, 292, 389, 418
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HincII GTYRAC 1 cut(s) 764
HindII GTYRAC 1 cut(s) 764
HindIII AAGCTT 1 cut(s) 439
HinfI GANTC 4 cut(s) 390, 534, 573, 601
HpaII CCGG 2 cut(s) 778, 828
HphI GGTGA 4 cut(s) 139, 371, 526, 694
Hpy166II GTNNAC 4 cut(s) 268, 421, 629, 764
Hpy188I TCNGA 5 cut(s) 75, 373, 498, 652, 719
Hpy188III TCNNGA 2 cut(s) 742, 753
Hpy8I GTNNAC 4 cut(s) 268, 421, 629, 764
HpyAV CCTTC 3 cut(s) 74, 378, 496
HpyCH4III ACNGT 2 cut(s) 236, 759
HpyCH4IV ACGT 3 cut(s) 273, 595, 657
HpyCH4V TGCA 8 cut(s) 54, 59, 65, 172, 349, 355, 401, 412
HpyF10VI GCNNNNNNNGC 1 cut(s) 452
HpyF3I CTNAG 4 cut(s) 72, 405, 495, 716
HpySE526I ACGT 3 cut(s) 273, 595, 657
Hsp92II CATG 5 cut(s) 43, 156, 292, 389, 418
HspAI GCGC 1 cut(s) 156
Kzo9I GATC 1 cut(s) 719
LguI GCTCTTC 1 cut(s) 474
LmnI GCTCC 2 cut(s) 305, 653
LpnPI CCDG 4 cut(s) 75, 294, 791, 841
Lsp1109I GCAGC 2 cut(s) 396, 413
LweI GCATC 1 cut(s) 58
MaeI CTAG 1 cut(s) 263
MaeII ACGT 3 cut(s) 273, 595, 657
MaeIII GTNAC 1 cut(s) 830
MalI GATC 1 cut(s) 721
MboI GATC 1 cut(s) 719
MboII GAAGA 7 cut(s) 16, 25, 286, 454, 491, 539, 582
MfeI CAATTG 1 cut(s) 180
MflI RGATCY 1 cut(s) 719
MluCI AATT 7 cut(s) 167, 180, 218, 223, 509, 748, 850
MlyI GAGTC 3 cut(s) 399, 528, 595
MnlI CCTC 9 cut(s) 56, 150, 189, 295, 392, 500, 651, 678, 779
Mph1103I ATGCAT 1 cut(s) 357
MseI TTAA 3 cut(s) 21, 134, 853
MslI CAYNNNNRTG 2 cut(s) 384, 807
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 2 cut(s) 778, 828
MunI CAATTG 1 cut(s) 180
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 1 cut(s) 452
NcoI CCATGG 1 cut(s) 385
NdeII GATC 1 cut(s) 719
NlaIII CATG 5 cut(s) 43, 156, 292, 389, 418
NlaIV GGNNCC 1 cut(s) 545
NsiI ATGCAT 1 cut(s) 357
NspI RCATGY 1 cut(s) 156
OliI CACNNNNGTG 1 cut(s) 807
PaqCI CACCTGC 1 cut(s) 70
PciSI GCTCTTC 1 cut(s) 474
PcsI WCGNNNNNNNCGW 2 cut(s) 601, 810
PfeI GAWTC 1 cut(s) 573
PflFI GACNNNGTC 1 cut(s) 759
PinAI ACCGGT 1 cut(s) 827
PkrI GCNGC 2 cut(s) 403, 411
PleI GAGTC 3 cut(s) 398, 528, 595
PpsI GAGTC 3 cut(s) 398, 528, 595
Psp1406I AACGTT 1 cut(s) 657
PspN4I GGNNCC 1 cut(s) 545
PspPI GGNCC 2 cut(s) 113, 666
PstI CTGCAG 1 cut(s) 67
PsuI RGATCY 1 cut(s) 719
PsyI GACNNNGTC 1 cut(s) 759
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 2 cut(s) 420, 611
RsaNI GTAC 2 cut(s) 419, 610
RseI CAYNNNNRTG 2 cut(s) 384, 807
SapI GCTCTTC 1 cut(s) 474
SaqAI TTAA 3 cut(s) 21, 134, 853
SatI GCNGC 2 cut(s) 402, 410
Sau3AI GATC 1 cut(s) 719
Sau96I GGNCC 2 cut(s) 113, 666
ScaI AGTACT 1 cut(s) 611
SchI GAGTC 3 cut(s) 399, 528, 595
SfaNI GCATC 1 cut(s) 58
SfcI CTRYAG 2 cut(s) 63, 613
SinI GGWCC 2 cut(s) 113, 666
SmiMI CAYNNNNRTG 2 cut(s) 384, 807
SpeI ACTAGT 1 cut(s) 262
Sse9I AATT 7 cut(s) 167, 180, 218, 223, 509, 748, 850
SsiI CCGC 1 cut(s) 158
SspI AATATT 1 cut(s) 204
SspMI CTAG 1 cut(s) 263
StyI CCWWGG 2 cut(s) 385, 673
TaaI ACNGT 2 cut(s) 236, 759
TaiI ACGT 3 cut(s) 276, 598, 660
TaqI TCGA 3 cut(s) 576, 604, 813
TasI AATT 7 cut(s) 167, 180, 218, 223, 509, 748, 850
TatI WGTACW 1 cut(s) 609
TfiI GAWTC 1 cut(s) 573
Tru1I TTAA 3 cut(s) 21, 134, 853
Tru9I TTAA 3 cut(s) 21, 134, 853
TscAI CASTG 1 cut(s) 481
TseI GCWGC 2 cut(s) 401, 409
TspDTI ATGAA 2 cut(s) 17, 515
TspRI CASTG 1 cut(s) 481
Tth111I GACNNNGTC 1 cut(s) 759
VpaK11BI GGWCC 2 cut(s) 113, 666
XagI CCTNNNNNAGG 1 cut(s) 677
XapI RAATTY 1 cut(s) 223
XceI RCATGY 1 cut(s) 156
XcmI CCANNNNNNNNNTGG 1 cut(s) 431
XspI CTAG 1 cut(s) 263
ZrmI AGTACT 1 cut(s) 611
Zsp2I ATGCAT 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.