Rroxscaffold_6G00407730

Bifunctional monodehydroascorbate reductase and carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0010395
Physical Location & Seq
Reverse (-)
30381471 .. 30386388
4918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00407730.1

Sequence Viewer

Length: 816 bp
ATGGCTTCAAGAATTTCGGTGTCGATCCTCATGGTTACATTGCTGCTTGTTGGTTCTTCAGCTTGCTTTGTGGAAGAGCTGAAGTCGCTTGGATTTACTTACTCGGGTGCGACTGGCCCTCAGAGATGGGGAAGCTTGAGTCCATATTTCTCAGCATGTAATGGAAAGAAGCAATCTCCTCTGGACCTTGCCAAGGACAAGGTTATTCGTAGCAGGAATTTCAAGCCATTAACTCGAAATTACCTTGCGTTGAATGCCACATTATACAACAATGGCTTTAACATTGGGGTGCATTTCGATGGACAAATTGCCGGAACATTAGTCATAGATGGTAAGAATTACAACTTGAAGCAGCTGCACTGGCATTCTCCGTCTGAACACCGTATTAATGGAGTCCAATTTCCAGCGGAGCTTCATCTAGTTCACCTGGCAGATGATAATAGCATCGCAGTTGTGGCAATTCTTTTCCAATATGGCAAAGATGATATCTTCATCTCTCAGATCAAGGACAAGCTGGCGGAGTTGGCCGAGGAAGTATGTAGAAGAGATAAGGATGCACATATTCCCCTCGGAAATATAGACATGAAAGAGTTCGATAAAAGAACCCGCAAATATTGGAGATATGTTGGTTCTCTCTCTTCTCCTCCATGCACTGAGAATGTCATCTGGAACATCCTTGGAAAGGTGAGGTACATTTCCAAGGCTCAGATAGACGCTATAAAAGCACCATTGGGAGCAGATTACAAGAACAACTCGAGACCTCTGCAGCCTCTTAATGGACGCAAAATTGAGCTCTTCCATGAGCTTAGCAACTAG

Protein Analysis

271

Amino Acids

30.43

Weight (kDa)

9.24

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 39 - 264 3.9e-41 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52720 AT3G52720 AT3G52720 AT3G52720
fragaria_vesca FvH4_6g18830 FvH4_6g18830
malus_domestica MD12G1024600.v1.1
prunus_persica Prupe.7G109300_v2.0.a1 Prupe.7G109300_v2.0.a1
pyrus_communis pycom12g02430
rosa_chinensis RchiOBHm_Chr3g0473841
rosa_laevigata RLG00000023979
rosa_multiflora Rmu_sc0005561.1_g000003
rosa_roxburghii Rroxscaffold_6G00407730
rosa_rugosa Rorug03G0135900 Rorug03G0136000
rosa_samantha Rh3AG186400 Rh3BG215000 Rh3CG211700 Rh3DG210800
rosa_wichuraiana Rw3G017050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 407, 518, 607
AclWI GGATC 1 cut(s) 19
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 2 cut(s) 12, 217
AcuI CTGAAG 2 cut(s) 42, 101
AfaI GTAC 1 cut(s) 692
AfiI CCNNNNNNNGG 3 cut(s) 193, 682, 776
AgsI TTSAA 4 cut(s) 9, 223, 253, 349
AjnI CCWGG 1 cut(s) 426
AluBI AGCT 8 cut(s) 62, 79, 135, 355, 412, 514, 793, 805
AluI AGCT 8 cut(s) 62, 79, 135, 355, 412, 514, 793, 805
Alw21I GWGCWC 1 cut(s) 795
Alw26I GTCTC 1 cut(s) 751
AlwI GGATC 1 cut(s) 19
Ama87I CYCGRG 2 cut(s) 103, 754
AoxI GGCC 2 cut(s) 115, 525
ApeKI GCWGC 4 cut(s) 43, 352, 355, 766
ApoI RAATTY 2 cut(s) 12, 217
AseI ATTAAT 1 cut(s) 387
Asp700I GAANNNNTTC 1 cut(s) 590
AspS9I GGNCC 2 cut(s) 116, 184
AsuHPI GGTGA 2 cut(s) 416, 697
AvaI CYCGRG 2 cut(s) 103, 754
AvaII GGWCC 1 cut(s) 184
BanII GRGCYC 1 cut(s) 795
Bbv12I GWGCWC 1 cut(s) 795
BbvI GCAGC 4 cut(s) 30, 342, 364, 778
BccI CCATC 3 cut(s) 120, 293, 323
BcgI CGANNNNNNTGC 2 cut(s) 745, 779
BciT130I CCWGG 1 cut(s) 428
BcoDI GTCTC 1 cut(s) 751
BfaI CTAG 2 cut(s) 419, 814
BfmI CTRYAG 1 cut(s) 764
BisI GCNGC 4 cut(s) 44, 353, 356, 767
BlpI GCTNAGC 1 cut(s) 806
BlsI GCNGC 4 cut(s) 45, 354, 357, 768
Bme1390I CCNGG 1 cut(s) 428
Bme18I GGWCC 1 cut(s) 184
BmeT110I CYCGRG 2 cut(s) 103, 754
BmgT120I GGNCC 2 cut(s) 116, 184
BmrFI CCNGG 1 cut(s) 428
BmsI GCATC 2 cut(s) 453, 544
Bpu1102I GCTNAGC 1 cut(s) 806
BpuEI CTTGAG 1 cut(s) 157
BsaI GGTCTC 1 cut(s) 751
BsaJI CCNNGG 5 cut(s) 192, 528, 568, 676, 699
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 682, 776
Bse1I ACTGG 2 cut(s) 118, 365
Bse3DI GCAATG 1 cut(s) 38
BseBI CCWGG 1 cut(s) 428
BseDI CCNNGG 5 cut(s) 192, 528, 568, 676, 699
BseGI GGATG 2 cut(s) 559, 672
BseLI CCNNNNNNNGG 3 cut(s) 193, 682, 776
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 5 cut(s) 134, 165, 512, 645, 719
BseNI ACTGG 2 cut(s) 118, 365
BseRI GAGGAG 2 cut(s) 168, 633
BseXI GCAGC 4 cut(s) 30, 342, 364, 778
BsgI GTGCAG 1 cut(s) 341
BshFI GGCC 2 cut(s) 117, 527
BsiHKAI GWGCWC 1 cut(s) 795
BsiHKCI CYCGRG 2 cut(s) 103, 754
BsiSI CCGG 1 cut(s) 312
BslI CCNNNNNNNGG 3 cut(s) 193, 682, 776
BsmAI GTCTC 1 cut(s) 751
BsmI GAATGC 2 cut(s) 259, 364
BsnI GGCC 2 cut(s) 117, 527
Bso31I GGTCTC 1 cut(s) 751
BsoBI CYCGRG 2 cut(s) 103, 754
Bsp1286I GDGCHC 1 cut(s) 795
Bsp143I GATC 2 cut(s) 24, 501
Bsp1720I GCTNAGC 1 cut(s) 806
BspACI CCGC 3 cut(s) 407, 518, 607
BspANI GGCC 2 cut(s) 117, 527
BspCNI CTCAG 5 cut(s) 133, 164, 511, 646, 718
BspMAI CTGCAG 1 cut(s) 768
BspPI GGATC 1 cut(s) 19
BspQI GCTCTTC 2 cut(s) 69, 800
BspTNI GGTCTC 1 cut(s) 751
BsrDI GCAATG 1 cut(s) 38
BsrI ACTGG 2 cut(s) 118, 365
BssECI CCNNGG 5 cut(s) 192, 528, 568, 676, 699
BssMI GATC 2 cut(s) 24, 501
BssT1I CCWWGG 3 cut(s) 192, 676, 699
Bst2UI CCWGG 1 cut(s) 428
Bst4CI ACNGT 1 cut(s) 383
Bst6I CTCTTC 4 cut(s) 69, 538, 643, 800
BstC8I GCNNGC 2 cut(s) 64, 516
BstDEI CTNAG 6 cut(s) 120, 151, 498, 654, 705, 806
BstENI CCTNNNNNAGG 2 cut(s) 191, 680
BstF5I GGATG 2 cut(s) 559, 672
BstKTI GATC 2 cut(s) 27, 504
BstMAI GTCTC 1 cut(s) 751
BstMBI GATC 2 cut(s) 24, 501
BstMWI GCNNNNNNNGC 6 cut(s) 85, 254, 361, 455, 524, 722
BstNI CCWGG 1 cut(s) 428
BstNSI RCATGY 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 426
BstSFI CTRYAG 1 cut(s) 764
BstV1I GCAGC 4 cut(s) 30, 342, 364, 778
BsuRI GGCC 2 cut(s) 117, 527
BtgZI GCGATG 1 cut(s) 430
BtsCI GGATG 2 cut(s) 559, 672
BtsIMutI CAGTG 2 cut(s) 358, 651
Cac8I GCNNGC 2 cut(s) 64, 516
Cfr13I GGNCC 2 cut(s) 116, 184
CseI GACGC 2 cut(s) 722, 789
Csp6I GTAC 1 cut(s) 691
CviAII CATG 5 cut(s) 31, 156, 583, 648, 800
CviQI GTAC 1 cut(s) 691
DdeI CTNAG 6 cut(s) 120, 151, 498, 654, 705, 806
DpnI GATC 2 cut(s) 26, 503
DpnII GATC 2 cut(s) 24, 501
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 4 cut(s) 69, 538, 643, 800
EarI CTCTTC 4 cut(s) 69, 538, 643, 800
EciI GGCGGA 1 cut(s) 533
Ecl136II GAGCTC 1 cut(s) 793
Eco130I CCWWGG 3 cut(s) 192, 676, 699
Eco24I GRGCYC 1 cut(s) 795
Eco31I GGTCTC 1 cut(s) 751
Eco32I GATATC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 184
Eco53kI GAGCTC 1 cut(s) 793
Eco57I CTGAAG 2 cut(s) 42, 101
Eco88I CYCGRG 2 cut(s) 103, 754
EcoICRI GAGCTC 1 cut(s) 793
EcoNI CCTNNNNNAGG 2 cut(s) 191, 680
EcoRII CCWGG 1 cut(s) 426
EcoRV GATATC 1 cut(s) 487
EcoT14I CCWWGG 3 cut(s) 192, 676, 699
EcoT38I GRGCYC 1 cut(s) 795
ErhI CCWWGG 3 cut(s) 192, 676, 699
FaeI CATG 5 cut(s) 34, 159, 586, 651, 803
FatI CATG 5 cut(s) 30, 155, 582, 647, 799
FauI CCCGC 1 cut(s) 614
Fnu4HI GCNGC 4 cut(s) 44, 353, 356, 767
FokI GGATG 2 cut(s) 566, 659
FriOI GRGCYC 1 cut(s) 795
Fsp4HI GCNGC 4 cut(s) 44, 353, 356, 767
FspBI CTAG 2 cut(s) 419, 814
GluI GCNGC 4 cut(s) 44, 353, 356, 767
HaeIII GGCC 2 cut(s) 117, 527
HapII CCGG 1 cut(s) 312
HgaI GACGC 2 cut(s) 722, 789
Hin1II CATG 5 cut(s) 34, 159, 586, 651, 803
HindIII AAGCTT 1 cut(s) 133
HinfI GANTC 2 cut(s) 139, 393
HpaII CCGG 1 cut(s) 312
HphI GGTGA 2 cut(s) 416, 697
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 5 cut(s) 123, 376, 501, 572, 708
Hpy188III TCNNGA 4 cut(s) 9, 182, 667, 756
Hpy8I GTNNAC 1 cut(s) 424
HpyCH4III ACNGT 1 cut(s) 383
HpyCH4V TGCA 5 cut(s) 292, 358, 557, 651, 766
HpyF10VI GCNNNNNNNGC 6 cut(s) 85, 254, 361, 455, 524, 722
HpyF3I CTNAG 6 cut(s) 120, 151, 498, 654, 705, 806
Hsp92II CATG 5 cut(s) 34, 159, 586, 651, 803
Kzo9I GATC 2 cut(s) 24, 501
LguI GCTCTTC 2 cut(s) 69, 800
LmnI GCTCC 2 cut(s) 409, 734
Lsp1109I GCAGC 4 cut(s) 30, 342, 364, 778
LweI GCATC 2 cut(s) 453, 544
MaeI CTAG 2 cut(s) 419, 814
MaeIII GTNAC 1 cut(s) 34
MalI GATC 2 cut(s) 26, 503
MboI GATC 2 cut(s) 24, 501
MboII GAAGA 6 cut(s) 48, 86, 481, 555, 630, 787
MhlI GDGCHC 1 cut(s) 795
MluCI AATT 8 cut(s) 12, 217, 238, 306, 337, 398, 459, 786
MlyI GAGTC 2 cut(s) 148, 402
MnlI CCTC 9 cut(s) 38, 129, 189, 523, 578, 654, 681, 771, 780
MroXI GAANNNNTTC 1 cut(s) 590
MseI TTAA 4 cut(s) 230, 279, 387, 774
MslI CAYNNNNRTG 2 cut(s) 287, 297
MspA1I CMGCKG 2 cut(s) 355, 407
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 1 cut(s) 428
Mva1269I GAATGC 2 cut(s) 259, 364
MvaI CCWGG 1 cut(s) 428
MwoI GCNNNNNNNGC 6 cut(s) 85, 254, 361, 455, 524, 722
NdeII GATC 2 cut(s) 24, 501
NlaIII CATG 5 cut(s) 34, 159, 586, 651, 803
NmeAIII GCCGAG 1 cut(s) 553
NspI RCATGY 1 cut(s) 159
PaeR7I CTCGAG 1 cut(s) 754
PciSI GCTCTTC 2 cut(s) 69, 800
PctI GAATGC 2 cut(s) 259, 364
PdmI GAANNNNTTC 1 cut(s) 590
PkrI GCNGC 4 cut(s) 45, 354, 357, 768
PleI GAGTC 2 cut(s) 147, 401
PpsI GAGTC 2 cut(s) 147, 401
PshBI ATTAAT 1 cut(s) 387
Psp124BI GAGCTC 1 cut(s) 795
Psp6I CCWGG 1 cut(s) 426
PspGI CCWGG 1 cut(s) 426
PspPI GGNCC 2 cut(s) 116, 184
PstI CTGCAG 1 cut(s) 768
PvuII CAGCTG 1 cut(s) 355
RsaI GTAC 1 cut(s) 692
RsaNI GTAC 1 cut(s) 691
RseI CAYNNNNRTG 2 cut(s) 287, 297
SacI GAGCTC 1 cut(s) 795
SapI GCTCTTC 2 cut(s) 69, 800
SaqAI TTAA 4 cut(s) 230, 279, 387, 774
SatI GCNGC 4 cut(s) 44, 353, 356, 767
Sau3AI GATC 2 cut(s) 24, 501
Sau96I GGNCC 2 cut(s) 116, 184
SchI GAGTC 2 cut(s) 148, 402
ScrFI CCNGG 1 cut(s) 428
SduI GDGCHC 1 cut(s) 795
SfaNI GCATC 2 cut(s) 453, 544
SfcI CTRYAG 1 cut(s) 764
Sfr274I CTCGAG 1 cut(s) 754
SinI GGWCC 1 cut(s) 184
SlaI CTCGAG 1 cut(s) 754
SmiMI CAYNNNNRTG 2 cut(s) 287, 297
SmlI CTYRAG 2 cut(s) 136, 754
SmoI CTYRAG 2 cut(s) 136, 754
Sse9I AATT 8 cut(s) 12, 217, 238, 306, 337, 398, 459, 786
SsiI CCGC 3 cut(s) 407, 518, 607
SspI AATATT 1 cut(s) 614
SspMI CTAG 2 cut(s) 419, 814
SstI GAGCTC 1 cut(s) 795
StyD4I CCNGG 1 cut(s) 426
StyI CCWWGG 3 cut(s) 192, 676, 699
TaaI ACNGT 1 cut(s) 383
TaqI TCGA 5 cut(s) 23, 235, 297, 594, 755
TasI AATT 8 cut(s) 12, 217, 238, 306, 337, 398, 459, 786
Tru1I TTAA 4 cut(s) 230, 279, 387, 774
Tru9I TTAA 4 cut(s) 230, 279, 387, 774
TscAI CASTG 2 cut(s) 365, 658
TseI GCWGC 4 cut(s) 43, 352, 355, 766
TspDTI ATGAA 3 cut(s) 404, 481, 599
TspGWI ACGGA 1 cut(s) 360
TspRI CASTG 2 cut(s) 365, 658
VpaK11BI GGWCC 1 cut(s) 184
VspI ATTAAT 1 cut(s) 387
XagI CCTNNNNNAGG 2 cut(s) 191, 680
XapI RAATTY 2 cut(s) 12, 217
XceI RCATGY 1 cut(s) 159
XhoI CTCGAG 1 cut(s) 754
XmnI GAANNNNTTC 1 cut(s) 590
XspI CTAG 2 cut(s) 419, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.