Rh3BG215000

Bifunctional monodehydroascorbate reductase and carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0010395
Physical Location & Seq
Forward (+)
19395960 .. 19400769
4810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG215000.1

Sequence Viewer

Length: 516 bp
ATGGGTACAAGAATTTCGGTGTCGATCCTCATGGTGACATTGCTGCTTGTTGGTTCTTCAGCTTGCTTTGTGGAAGAGCTGAAGTCGCTTGGATTTACTTACTCGGGTGCGACTGGCCCTCAGAGATGGGGAAACTTGAGTCCATATTTCTCAGCATGTAATGGAAAGAAGCAATCTCCTCTGGACCTTGCCAAGGACAAGGTTATTCGTAGCAGGAATTTCAAGCCATTAACCCGAAATTACCTTGCGTTGAATGCCACATTATACAACAATGGCTTTAACATTGGGGTGCATTTCGATGGACAAATTGCCGGAACATTAGTCATAGATGGTAAGAATTACAACTTGAAGCAGCTGCACTGGCATTCTCCATCTGAACACCGTATTAATGGAGTCCAGTATGTGCTCTCTCTCTCTCTCTCTCATAGCCAATATACTCCTCTTTCTGGTTTACTGATGACACTTGAAATTACAGAGGAGAATAGATTAGCACATGAATATTACAAACTGAGATGA

Protein Analysis

171

Amino Acids

19.14

Weight (kDa)

9.39

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 39 - 143 2.5e-14 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52720 AT3G52720 AT3G52720 AT3G52720
fragaria_vesca FvH4_6g18830 FvH4_6g18830
malus_domestica MD12G1024600.v1.1
prunus_persica Prupe.7G109300_v2.0.a1 Prupe.7G109300_v2.0.a1
pyrus_communis pycom12g02430
rosa_chinensis RchiOBHm_Chr3g0473841
rosa_laevigata RLG00000023979
rosa_multiflora Rmu_sc0005561.1_g000003
rosa_roxburghii Rroxscaffold_6G00407730
rosa_rugosa Rorug03G0135900 Rorug03G0136000
rosa_samantha Rh3AG186400 Rh3BG215000 Rh3CG211700 Rh3DG210800
rosa_wichuraiana Rw3G017050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 19
AcsI RAATTY 2 cut(s) 12, 217
AcuI CTGAAG 2 cut(s) 42, 101
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 2 cut(s) 193, 446
AgsI TTSAA 4 cut(s) 223, 253, 349, 467
AluBI AGCT 3 cut(s) 62, 79, 355
AluI AGCT 3 cut(s) 62, 79, 355
Alw21I GWGCWC 1 cut(s) 408
AlwI GGATC 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 103
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 3 cut(s) 43, 352, 355
ApoI RAATTY 2 cut(s) 12, 217
AseI ATTAAT 1 cut(s) 387
AspS9I GGNCC 2 cut(s) 116, 184
AsuHPI GGTGA 1 cut(s) 46
AvaI CYCGRG 1 cut(s) 103
AvaII GGWCC 1 cut(s) 184
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 3 cut(s) 30, 342, 364
BccI CCATC 4 cut(s) 120, 293, 323, 379
BisI GCNGC 3 cut(s) 44, 353, 356
BlsI GCNGC 3 cut(s) 45, 354, 357
Bme18I GGWCC 1 cut(s) 184
BmeT110I CYCGRG 1 cut(s) 103
BmgT120I GGNCC 2 cut(s) 116, 184
BpuEI CTTGAG 1 cut(s) 157
BsaJI CCNNGG 1 cut(s) 192
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 446
Bse1I ACTGG 3 cut(s) 118, 365, 397
Bse3DI GCAATG 1 cut(s) 38
BseDI CCNNGG 1 cut(s) 192
BseLI CCNNNNNNNGG 2 cut(s) 193, 446
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 3 cut(s) 134, 165, 500
BseNI ACTGG 3 cut(s) 118, 365, 397
BseRI GAGGAG 3 cut(s) 168, 429, 491
BseXI GCAGC 3 cut(s) 30, 342, 364
BsgI GTGCAG 1 cut(s) 341
BshFI GGCC 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 408
BsiHKCI CYCGRG 1 cut(s) 103
BsiSI CCGG 1 cut(s) 312
BslI CCNNNNNNNGG 2 cut(s) 193, 446
BsmI GAATGC 2 cut(s) 259, 364
BsnI GGCC 1 cut(s) 117
BsoBI CYCGRG 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 408
Bsp143I GATC 1 cut(s) 24
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 3 cut(s) 133, 164, 501
BspPI GGATC 1 cut(s) 19
BspQI GCTCTTC 1 cut(s) 69
BsrDI GCAATG 1 cut(s) 38
BsrI ACTGG 3 cut(s) 118, 365, 397
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 1 cut(s) 24
BssT1I CCWWGG 1 cut(s) 192
Bst4CI ACNGT 1 cut(s) 383
Bst6I CTCTTC 1 cut(s) 69
BstC8I GCNNGC 1 cut(s) 64
BstDEI CTNAG 3 cut(s) 120, 151, 509
BstENI CCTNNNNNAGG 1 cut(s) 191
BstKTI GATC 1 cut(s) 27
BstMBI GATC 1 cut(s) 24
BstMWI GCNNNNNNNGC 3 cut(s) 85, 254, 361
BstNSI RCATGY 1 cut(s) 159
BstV1I GCAGC 3 cut(s) 30, 342, 364
BsuRI GGCC 1 cut(s) 117
BtsIMutI CAGTG 1 cut(s) 358
Cac8I GCNNGC 1 cut(s) 64
Cfr13I GGNCC 2 cut(s) 116, 184
Csp6I GTAC 1 cut(s) 6
CviAII CATG 3 cut(s) 31, 156, 494
CviJI RGCY 7 cut(s) 62, 79, 117, 226, 276, 355, 429
CviKI_1 RGCY 7 cut(s) 62, 79, 117, 226, 276, 355, 429
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 3 cut(s) 120, 151, 509
DpnI GATC 1 cut(s) 26
DpnII GATC 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 192
Eco47I GGWCC 1 cut(s) 184
Eco57I CTGAAG 2 cut(s) 42, 101
Eco88I CYCGRG 1 cut(s) 103
EcoNI CCTNNNNNAGG 1 cut(s) 191
EcoT14I CCWWGG 1 cut(s) 192
ErhI CCWWGG 1 cut(s) 192
FaeI CATG 3 cut(s) 34, 159, 497
FaiI YATR 9 cut(s) 32, 145, 157, 265, 326, 402, 426, 435, 495
FatI CATG 3 cut(s) 30, 155, 493
Fnu4HI GCNGC 3 cut(s) 44, 353, 356
Fsp4HI GCNGC 3 cut(s) 44, 353, 356
GluI GCNGC 3 cut(s) 44, 353, 356
HaeIII GGCC 1 cut(s) 117
HapII CCGG 1 cut(s) 312
Hin1II CATG 3 cut(s) 34, 159, 497
HinfI GANTC 2 cut(s) 139, 393
HpaII CCGG 1 cut(s) 312
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 1 cut(s) 452
Hpy188I TCNGA 2 cut(s) 123, 376
Hpy188III TCNNGA 1 cut(s) 182
Hpy8I GTNNAC 1 cut(s) 452
HpyCH4III ACNGT 1 cut(s) 383
HpyCH4V TGCA 2 cut(s) 292, 358
HpyF10VI GCNNNNNNNGC 3 cut(s) 85, 254, 361
HpyF3I CTNAG 3 cut(s) 120, 151, 509
Hsp92II CATG 3 cut(s) 34, 159, 497
Kzo9I GATC 1 cut(s) 24
LguI GCTCTTC 1 cut(s) 69
LpnPI CCDG 7 cut(s) 99, 167, 199, 325, 346, 410, 432
Lsp1109I GCAGC 3 cut(s) 30, 342, 364
MaeIII GTNAC 1 cut(s) 34
MalI GATC 1 cut(s) 26
MboI GATC 1 cut(s) 24
MboII GAAGA 2 cut(s) 48, 86
MhlI GDGCHC 1 cut(s) 408
MluCI AATT 6 cut(s) 12, 217, 238, 306, 337, 468
MlyI GAGTC 2 cut(s) 148, 402
MnlI CCTC 5 cut(s) 38, 129, 189, 450, 469
MseI TTAA 3 cut(s) 230, 279, 387
MslI CAYNNNNRTG 2 cut(s) 287, 297
MspA1I CMGCKG 1 cut(s) 355
MspI CCGG 1 cut(s) 312
Mva1269I GAATGC 2 cut(s) 259, 364
MwoI GCNNNNNNNGC 3 cut(s) 85, 254, 361
NdeII GATC 1 cut(s) 24
NlaIII CATG 3 cut(s) 34, 159, 497
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 1 cut(s) 159
PciSI GCTCTTC 1 cut(s) 69
PctI GAATGC 2 cut(s) 259, 364
PkrI GCNGC 3 cut(s) 45, 354, 357
PleI GAGTC 2 cut(s) 147, 401
PpsI GAGTC 2 cut(s) 147, 401
PshBI ATTAAT 1 cut(s) 387
PspPI GGNCC 2 cut(s) 116, 184
PvuII CAGCTG 1 cut(s) 355
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
RseI CAYNNNNRTG 2 cut(s) 287, 297
SapI GCTCTTC 1 cut(s) 69
SaqAI TTAA 3 cut(s) 230, 279, 387
SatI GCNGC 3 cut(s) 44, 353, 356
Sau3AI GATC 1 cut(s) 24
Sau96I GGNCC 2 cut(s) 116, 184
SchI GAGTC 2 cut(s) 148, 402
SduI GDGCHC 1 cut(s) 408
SetI ASST 6 cut(s) 64, 81, 189, 204, 246, 357
SinI GGWCC 1 cut(s) 184
SmiMI CAYNNNNRTG 2 cut(s) 287, 297
SmlI CTYRAG 1 cut(s) 136
SmoI CTYRAG 1 cut(s) 136
Sse9I AATT 6 cut(s) 12, 217, 238, 306, 337, 468
SspI AATATT 1 cut(s) 500
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 1 cut(s) 383
TaqI TCGA 2 cut(s) 23, 297
TasI AATT 6 cut(s) 12, 217, 238, 306, 337, 468
Tru1I TTAA 3 cut(s) 230, 279, 387
Tru9I TTAA 3 cut(s) 230, 279, 387
TscAI CASTG 1 cut(s) 365
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 3 cut(s) 43, 352, 355
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 1 cut(s) 510
TspRI CASTG 1 cut(s) 365
VpaK11BI GGWCC 1 cut(s) 184
VspI ATTAAT 1 cut(s) 387
XagI CCTNNNNNAGG 1 cut(s) 191
XapI RAATTY 2 cut(s) 12, 217
XceI RCATGY 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.