FvH4_6g18830

Bifunctional monodehydroascorbate reductase and carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010395
Physical Location & Seq
Forward (+)
12399912 .. 12403304
3393 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18830.t2

Sequence Viewer

Length: 813 bp
ATGGCTCTCAGAGTTTCATTGTTGATGCTCATGGTGACATTGCTGCTTGTTGGTTCTTCAGCTTGCTTTGTAGAAGAGCTGAAGTCGATTGGATTTACTTACTCGGGTGCGACTGGCCCTCAGAGATGGGGAAGATTGAGTCCATATTTCTCAGCATGTAATGGAAAGAGGCAATCTCCTCTGAACCTTGCCAAGGACCATGTTGTTCGTATGAGCAATTTCAAGCCATTAACCCGAAACTATCTTGCGTTGAATGCCACATTATACAACAATGGCTTTAACATTGGGGTGCATTTCGATGGACAAATTGCAGGAACATTAGACATAGATGGTAAGAACTACAACTTGAAGCAGCTGCACTGGCATTCTCCCTCTGAACACCGTATTAACGGAGTCCAATTTCCAGCAGAGCTTCATCTAGTTCACCTCGCAGATGATAATAGCATTGCAGTTGTGGCGACTCTTTTCCAATATGGCAAAAACGATTCCTTCATCTCTCAGATCAAAGACCAGCTGGCGGAGTTGAATGAGGAGGTATGTAGAAGAGATAAGGATGCTCATGTTCCCCTTGGAAATATAGACATGAAGGAGTTCGATAAAAAAACCCGCAAATATTGGAGATATGTTGGCTCACTCTCCTCTCCTCCATGTACTGAGAATGTCGTTTGGAACATCCTTGGAAAGGTGAGGTACATTTCCAAGGCTCAGATAGAAGCTTTAAAAGCACCACTGGGAGTAGATTACAAGAACAACGCCAGACCACTGCAGCCTCTTAATGGACGCAAAATTGAACTCTTCGAGGAGCTTAGCTAA

Protein Analysis

271

Amino Acids

30.44

Weight (kDa)

9.06

Isoelectric Point (pI)

45.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 39 - 244 1.3e-34 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52720 AT3G52720 AT3G52720 AT3G52720
fragaria_vesca FvH4_6g18830 FvH4_6g18830
malus_domestica MD12G1024600.v1.1
prunus_persica Prupe.7G109300_v2.0.a1 Prupe.7G109300_v2.0.a1
pyrus_communis pycom12g02430
rosa_chinensis RchiOBHm_Chr3g0473841
rosa_laevigata RLG00000023979
rosa_multiflora Rmu_sc0005561.1_g000003
rosa_roxburghii Rroxscaffold_6G00407730
rosa_rugosa Rorug03G0135900 Rorug03G0136000
rosa_samantha Rh3AG186400 Rh3BG215000 Rh3CG211700 Rh3DG210800
rosa_wichuraiana Rw3G017050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 518, 607
AcuI CTGAAG 2 cut(s) 42, 101
AfaI GTAC 2 cut(s) 652, 692
AfiI CCNNNNNNNGG 4 cut(s) 193, 517, 682, 776
AgsI TTSAA 5 cut(s) 223, 253, 349, 526, 791
AluBI AGCT 8 cut(s) 62, 79, 355, 412, 514, 716, 805, 810
AluI AGCT 8 cut(s) 62, 79, 355, 412, 514, 716, 805, 810
Ama87I CYCGRG 1 cut(s) 103
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 4 cut(s) 43, 352, 355, 766
Asp700I GAANNNNTTC 1 cut(s) 590
AspS9I GGNCC 2 cut(s) 116, 196
AsuHPI GGTGA 3 cut(s) 46, 416, 697
AvaI CYCGRG 1 cut(s) 103
AvaII GGWCC 1 cut(s) 196
BbvI GCAGC 4 cut(s) 30, 342, 364, 778
BccI CCATC 3 cut(s) 120, 293, 323
BfaI CTAG 1 cut(s) 419
BfmI CTRYAG 1 cut(s) 764
BisI GCNGC 4 cut(s) 44, 353, 356, 767
BlpI GCTNAGC 1 cut(s) 806
BlsI GCNGC 4 cut(s) 45, 354, 357, 768
Bme18I GGWCC 1 cut(s) 196
BmeT110I CYCGRG 1 cut(s) 103
BmgT120I GGNCC 2 cut(s) 116, 196
BmrI ACTGGG 1 cut(s) 740
BmsI GCATC 2 cut(s) 15, 544
BmuI ACTGGG 1 cut(s) 740
BplI GAGNNNNNCTC 2 cut(s) 160, 192
Bpu1102I GCTNAGC 1 cut(s) 806
BsaJI CCNNGG 4 cut(s) 192, 568, 676, 699
Bsc4I CCNNNNNNNGG 4 cut(s) 193, 517, 682, 776
Bse1I ACTGG 3 cut(s) 118, 365, 735
Bse3DI GCAATG 2 cut(s) 38, 444
BseDI CCNNGG 4 cut(s) 192, 568, 676, 699
BseGI GGATG 2 cut(s) 559, 672
BseLI CCNNNNNNNGG 4 cut(s) 193, 517, 682, 776
BseMI GCAATG 2 cut(s) 38, 444
BseMII CTCAG 6 cut(s) 22, 134, 165, 512, 645, 719
BseNI ACTGG 3 cut(s) 118, 365, 735
BseRI GAGGAG 4 cut(s) 168, 545, 628, 633
BseXI GCAGC 4 cut(s) 30, 342, 364, 778
BsgI GTGCAG 1 cut(s) 341
BshFI GGCC 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 103
BslI CCNNNNNNNGG 4 cut(s) 193, 517, 682, 776
BsmI GAATGC 2 cut(s) 259, 364
BsnI GGCC 1 cut(s) 117
BsoBI CYCGRG 1 cut(s) 103
Bsp143I GATC 1 cut(s) 501
Bsp1720I GCTNAGC 1 cut(s) 806
BspACI CCGC 2 cut(s) 518, 607
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 6 cut(s) 21, 133, 164, 511, 646, 718
BspMAI CTGCAG 1 cut(s) 768
BspQI GCTCTTC 1 cut(s) 69
BsrDI GCAATG 2 cut(s) 38, 444
BsrI ACTGG 3 cut(s) 118, 365, 735
BssECI CCNNGG 4 cut(s) 192, 568, 676, 699
BssMI GATC 1 cut(s) 501
BssT1I CCWWGG 4 cut(s) 192, 568, 676, 699
Bst4CI ACNGT 1 cut(s) 383
Bst6I CTCTTC 3 cut(s) 69, 538, 800
BstC8I GCNNGC 2 cut(s) 64, 516
BstDEI CTNAG 7 cut(s) 8, 120, 151, 498, 654, 705, 806
BstENI CCTNNNNNAGG 2 cut(s) 191, 680
BstF5I GGATG 2 cut(s) 559, 672
BstKTI GATC 1 cut(s) 504
BstMBI GATC 1 cut(s) 501
BstMWI GCNNNNNNNGC 4 cut(s) 254, 361, 455, 722
BstNSI RCATGY 1 cut(s) 159
BstSFI CTRYAG 1 cut(s) 764
BstV1I GCAGC 4 cut(s) 30, 342, 364, 778
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 2 cut(s) 559, 672
BtsI GCAGTG 1 cut(s) 761
BtsIMutI CAGTG 3 cut(s) 358, 728, 761
Cac8I GCNNGC 2 cut(s) 64, 516
Cfr13I GGNCC 2 cut(s) 116, 196
CseI GACGC 1 cut(s) 789
Csp6I GTAC 2 cut(s) 651, 691
CviAII CATG 6 cut(s) 31, 156, 200, 560, 583, 648
CviQI GTAC 2 cut(s) 651, 691
DdeI CTNAG 7 cut(s) 8, 120, 151, 498, 654, 705, 806
DpnI GATC 1 cut(s) 503
DpnII GATC 1 cut(s) 501
DraI TTTAAA 1 cut(s) 720
Eam1104I CTCTTC 3 cut(s) 69, 538, 800
EarI CTCTTC 3 cut(s) 69, 538, 800
EciI GGCGGA 1 cut(s) 533
Eco130I CCWWGG 4 cut(s) 192, 568, 676, 699
Eco47I GGWCC 1 cut(s) 196
Eco57I CTGAAG 2 cut(s) 42, 101
Eco88I CYCGRG 1 cut(s) 103
EcoNI CCTNNNNNAGG 2 cut(s) 191, 680
EcoT14I CCWWGG 4 cut(s) 192, 568, 676, 699
ErhI CCWWGG 4 cut(s) 192, 568, 676, 699
FaeI CATG 6 cut(s) 34, 159, 203, 563, 586, 651
FatI CATG 6 cut(s) 30, 155, 199, 559, 582, 647
FauI CCCGC 1 cut(s) 614
Fnu4HI GCNGC 4 cut(s) 44, 353, 356, 767
FokI GGATG 2 cut(s) 566, 659
Fsp4HI GCNGC 4 cut(s) 44, 353, 356, 767
FspBI CTAG 1 cut(s) 419
GluI GCNGC 4 cut(s) 44, 353, 356, 767
HaeIII GGCC 1 cut(s) 117
HgaI GACGC 1 cut(s) 789
Hin1II CATG 6 cut(s) 34, 159, 203, 563, 586, 651
HindIII AAGCTT 1 cut(s) 714
HinfI GANTC 4 cut(s) 139, 393, 460, 485
HphI GGTGA 3 cut(s) 46, 416, 697
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 6 cut(s) 11, 123, 183, 376, 501, 708
Hpy8I GTNNAC 1 cut(s) 424
HpyAV CCTTC 2 cut(s) 499, 580
HpyCH4III ACNGT 1 cut(s) 383
HpyCH4V TGCA 5 cut(s) 292, 311, 358, 449, 766
HpyF10VI GCNNNNNNNGC 4 cut(s) 254, 361, 455, 722
HpyF3I CTNAG 7 cut(s) 8, 120, 151, 498, 654, 705, 806
Hsp92II CATG 6 cut(s) 34, 159, 203, 563, 586, 651
Kzo9I GATC 1 cut(s) 501
LguI GCTCTTC 1 cut(s) 69
LmnI GCTCC 1 cut(s) 802
LpnPI CCDG 8 cut(s) 99, 297, 346, 417, 500, 524, 716, 769
Lsp1109I GCAGC 4 cut(s) 30, 342, 364, 778
LweI GCATC 2 cut(s) 15, 544
MaeI CTAG 1 cut(s) 419
MaeIII GTNAC 1 cut(s) 34
MalI GATC 1 cut(s) 503
MboI GATC 1 cut(s) 501
MboII GAAGA 5 cut(s) 48, 86, 144, 555, 787
MluCI AATT 4 cut(s) 217, 306, 398, 786
MlyI GAGTC 3 cut(s) 148, 402, 454
MroXI GAANNNNTTC 1 cut(s) 590
MseI TTAA 5 cut(s) 230, 279, 387, 719, 774
MslI CAYNNNNRTG 2 cut(s) 287, 297
MspA1I CMGCKG 2 cut(s) 355, 514
Mva1269I GAATGC 2 cut(s) 259, 364
MwoI GCNNNNNNNGC 4 cut(s) 254, 361, 455, 722
NdeII GATC 1 cut(s) 501
NlaIII CATG 6 cut(s) 34, 159, 203, 563, 586, 651
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 1 cut(s) 159
PciSI GCTCTTC 1 cut(s) 69
PctI GAATGC 2 cut(s) 259, 364
PdmI GAANNNNTTC 1 cut(s) 590
PfeI GAWTC 1 cut(s) 485
PkrI GCNGC 4 cut(s) 45, 354, 357, 768
PleI GAGTC 3 cut(s) 147, 401, 454
PpsI GAGTC 3 cut(s) 147, 401, 454
PspPI GGNCC 2 cut(s) 116, 196
PstI CTGCAG 1 cut(s) 768
PvuII CAGCTG 2 cut(s) 355, 514
RsaI GTAC 2 cut(s) 652, 692
RsaNI GTAC 2 cut(s) 651, 691
RseI CAYNNNNRTG 2 cut(s) 287, 297
SapI GCTCTTC 1 cut(s) 69
SaqAI TTAA 5 cut(s) 230, 279, 387, 719, 774
SatI GCNGC 4 cut(s) 44, 353, 356, 767
Sau3AI GATC 1 cut(s) 501
Sau96I GGNCC 2 cut(s) 116, 196
SchI GAGTC 3 cut(s) 148, 402, 454
SfaNI GCATC 2 cut(s) 15, 544
SfcI CTRYAG 1 cut(s) 764
SinI GGWCC 1 cut(s) 196
SmiMI CAYNNNNRTG 2 cut(s) 287, 297
Sse9I AATT 4 cut(s) 217, 306, 398, 786
SsiI CCGC 2 cut(s) 518, 607
SspI AATATT 1 cut(s) 614
SspMI CTAG 1 cut(s) 419
StyI CCWWGG 4 cut(s) 192, 568, 676, 699
TaaI ACNGT 1 cut(s) 383
TaqI TCGA 4 cut(s) 86, 297, 594, 798
TasI AATT 4 cut(s) 217, 306, 398, 786
TatI WGTACW 1 cut(s) 650
TfiI GAWTC 1 cut(s) 485
Tru1I TTAA 5 cut(s) 230, 279, 387, 719, 774
Tru9I TTAA 5 cut(s) 230, 279, 387, 719, 774
TscAI CASTG 3 cut(s) 365, 735, 768
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 4 cut(s) 43, 352, 355, 766
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 4 cut(s) 6, 404, 481, 599
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 3 cut(s) 365, 735, 768
VpaK11BI GGWCC 1 cut(s) 196
XagI CCTNNNNNAGG 2 cut(s) 191, 680
XceI RCATGY 1 cut(s) 159
XmnI GAANNNNTTC 1 cut(s) 590
XspI CTAG 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.