RLG00000023979

Bifunctional monodehydroascorbate reductase and carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0010395
Physical Location & Seq
Reverse (-)
30658561 .. 30661849
3289 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023979

Sequence Viewer

Length: 765 bp
ATGGCTTCAAGAATTTCGGTGTCGATCCTCATGGTGACATTGCTGCTTGTTGGTTCTTCAGCTTGCTTTGTGGAAGAGCTGAAGTCGCTTGGATTTACTTACTCGGGTGCGACTGGCCCTCAGAGATGGGGAAGCTTGAGTCCATATTTCTCAGCATGTAATGGAAAGAAACAATCTCCTCTGGACCTTGCCAAGGACAAGGTTATTCGTAGCAGGAATTTCAAGCCATTAACCCGAAACTACCTTGCGTTGAATGCCACATTATACAACAATGGCTTTAACATTGGGGTGCATTTCGATGGACAAATTGCCGGAACATTAGTCATAGATGGTAAGAATTACAACTTGAAGCAGCTGCACTGGCATTCTCCATCTGAACACCGTATTAATGGAGTCCAATTTCCAGCGGAGCTTCATCTAGTTCACCTGGCAGATGATAATAGCATCGCAGTTGTGGCAATTCTTTTCCAATATGGCAAAGATGATATCTTCATCTCTCAGGATGCACATATTCCCCTCGGAAATATAGACATGAAAGAGTTCGATAAAAGAACCCGCAAATATTGGAGATATGTTGGCTCTCTCTCTTCTCCTCCATGCACTGAGAATGTCATCTGGAACATCCTTGGAAAGGTGAGGTACATTTCCAAGGCTCAGATAGACGCTATAAAAGCACCATTGGGAGCAGATTACAAGAACAACTCGAGACCGCTGCAGCCTCTTAATGGACGCAAAATTGAGCTCTTCCATAAGCTTAGCAACTAG

Protein Analysis

255

Amino Acids

28.43

Weight (kDa)

9.5

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 39 - 247 6.8e-37 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52720 AT3G52720 AT3G52720 AT3G52720
fragaria_vesca FvH4_6g18830 FvH4_6g18830
malus_domestica MD12G1024600.v1.1
prunus_persica Prupe.7G109300_v2.0.a1 Prupe.7G109300_v2.0.a1
pyrus_communis pycom12g02430
rosa_chinensis RchiOBHm_Chr3g0473841
rosa_laevigata RLG00000023979
rosa_multiflora Rmu_sc0005561.1_g000003
rosa_roxburghii Rroxscaffold_6G00407730
rosa_rugosa Rorug03G0135900 Rorug03G0136000
rosa_samantha Rh3AG186400 Rh3BG215000 Rh3CG211700 Rh3DG210800
rosa_wichuraiana Rw3G017050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 407, 556, 710
AclWI GGATC 1 cut(s) 19
AcsI RAATTY 2 cut(s) 12, 217
AcuI CTGAAG 2 cut(s) 42, 101
AfaI GTAC 1 cut(s) 641
AfiI CCNNNNNNNGG 3 cut(s) 193, 631, 725
AgsI TTSAA 4 cut(s) 9, 223, 253, 349
AjnI CCWGG 1 cut(s) 426
AluBI AGCT 7 cut(s) 62, 79, 135, 355, 412, 742, 754
AluI AGCT 7 cut(s) 62, 79, 135, 355, 412, 742, 754
Alw21I GWGCWC 1 cut(s) 744
Alw26I GTCTC 1 cut(s) 700
AlwI GGATC 1 cut(s) 19
Ama87I CYCGRG 2 cut(s) 103, 703
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 5 cut(s) 43, 352, 355, 712, 715
ApoI RAATTY 2 cut(s) 12, 217
AseI ATTAAT 1 cut(s) 387
Asp700I GAANNNNTTC 1 cut(s) 539
AspS9I GGNCC 2 cut(s) 116, 184
AsuHPI GGTGA 3 cut(s) 46, 416, 646
AvaI CYCGRG 2 cut(s) 103, 703
AvaII GGWCC 1 cut(s) 184
BanII GRGCYC 1 cut(s) 744
Bbv12I GWGCWC 1 cut(s) 744
BbvI GCAGC 5 cut(s) 30, 342, 364, 699, 727
BccI CCATC 4 cut(s) 120, 293, 323, 379
BcgI CGANNNNNNTGC 2 cut(s) 694, 728
BciT130I CCWGG 1 cut(s) 428
BcoDI GTCTC 1 cut(s) 700
BfaI CTAG 2 cut(s) 419, 763
BfmI CTRYAG 1 cut(s) 713
BisI GCNGC 5 cut(s) 44, 353, 356, 713, 716
BlpI GCTNAGC 1 cut(s) 755
BlsI GCNGC 5 cut(s) 45, 354, 357, 714, 717
Bme1390I CCNGG 1 cut(s) 428
Bme18I GGWCC 1 cut(s) 184
BmeT110I CYCGRG 2 cut(s) 103, 703
BmgT120I GGNCC 2 cut(s) 116, 184
BmrFI CCNGG 1 cut(s) 428
BmsI GCATC 2 cut(s) 453, 493
Bpu1102I GCTNAGC 1 cut(s) 755
BpuEI CTTGAG 1 cut(s) 157
BsaI GGTCTC 1 cut(s) 700
BsaJI CCNNGG 4 cut(s) 192, 517, 625, 648
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 631, 725
Bse1I ACTGG 2 cut(s) 118, 365
Bse3DI GCAATG 1 cut(s) 38
BseBI CCWGG 1 cut(s) 428
BseDI CCNNGG 4 cut(s) 192, 517, 625, 648
BseGI GGATG 2 cut(s) 508, 621
BseLI CCNNNNNNNGG 3 cut(s) 193, 631, 725
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 5 cut(s) 134, 165, 512, 594, 668
BseNI ACTGG 2 cut(s) 118, 365
BseRI GAGGAG 2 cut(s) 168, 582
BseXI GCAGC 5 cut(s) 30, 342, 364, 699, 727
BsgI GTGCAG 1 cut(s) 341
BshFI GGCC 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 744
BsiHKCI CYCGRG 2 cut(s) 103, 703
BsiSI CCGG 1 cut(s) 312
BslI CCNNNNNNNGG 3 cut(s) 193, 631, 725
BsmAI GTCTC 1 cut(s) 700
BsmI GAATGC 2 cut(s) 259, 364
BsnI GGCC 1 cut(s) 117
Bso31I GGTCTC 1 cut(s) 700
BsoBI CYCGRG 2 cut(s) 103, 703
Bsp1286I GDGCHC 1 cut(s) 744
Bsp143I GATC 1 cut(s) 24
Bsp1720I GCTNAGC 1 cut(s) 755
BspACI CCGC 3 cut(s) 407, 556, 710
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 5 cut(s) 133, 164, 511, 595, 667
BspMAI CTGCAG 1 cut(s) 717
BspPI GGATC 1 cut(s) 19
BspQI GCTCTTC 2 cut(s) 69, 749
BspTNI GGTCTC 1 cut(s) 700
BsrDI GCAATG 1 cut(s) 38
BsrI ACTGG 2 cut(s) 118, 365
BssECI CCNNGG 4 cut(s) 192, 517, 625, 648
BssMI GATC 1 cut(s) 24
BssT1I CCWWGG 3 cut(s) 192, 625, 648
Bst2UI CCWGG 1 cut(s) 428
Bst4CI ACNGT 1 cut(s) 383
Bst6I CTCTTC 3 cut(s) 69, 592, 749
BstC8I GCNNGC 1 cut(s) 64
BstDEI CTNAG 6 cut(s) 120, 151, 498, 603, 654, 755
BstENI CCTNNNNNAGG 2 cut(s) 191, 629
BstF5I GGATG 2 cut(s) 508, 621
BstKTI GATC 1 cut(s) 27
BstMAI GTCTC 1 cut(s) 700
BstMBI GATC 1 cut(s) 24
BstMWI GCNNNNNNNGC 5 cut(s) 85, 254, 361, 455, 671
BstNI CCWGG 1 cut(s) 428
BstNSI RCATGY 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 426
BstSFI CTRYAG 1 cut(s) 713
BstV1I GCAGC 5 cut(s) 30, 342, 364, 699, 727
BsuRI GGCC 1 cut(s) 117
BtgZI GCGATG 1 cut(s) 430
BtsCI GGATG 2 cut(s) 508, 621
BtsIMutI CAGTG 2 cut(s) 358, 600
Cac8I GCNNGC 1 cut(s) 64
Cfr13I GGNCC 2 cut(s) 116, 184
CseI GACGC 2 cut(s) 671, 738
Csp6I GTAC 1 cut(s) 640
CviAII CATG 4 cut(s) 31, 156, 532, 597
CviQI GTAC 1 cut(s) 640
DdeI CTNAG 6 cut(s) 120, 151, 498, 603, 654, 755
DpnI GATC 1 cut(s) 26
DpnII GATC 1 cut(s) 24
Eam1104I CTCTTC 3 cut(s) 69, 592, 749
EarI CTCTTC 3 cut(s) 69, 592, 749
Ecl136II GAGCTC 1 cut(s) 742
Eco130I CCWWGG 3 cut(s) 192, 625, 648
Eco24I GRGCYC 1 cut(s) 744
Eco31I GGTCTC 1 cut(s) 700
Eco32I GATATC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 184
Eco53kI GAGCTC 1 cut(s) 742
Eco57I CTGAAG 2 cut(s) 42, 101
Eco88I CYCGRG 2 cut(s) 103, 703
EcoICRI GAGCTC 1 cut(s) 742
EcoNI CCTNNNNNAGG 2 cut(s) 191, 629
EcoRII CCWGG 1 cut(s) 426
EcoRV GATATC 1 cut(s) 487
EcoT14I CCWWGG 3 cut(s) 192, 625, 648
EcoT38I GRGCYC 1 cut(s) 744
ErhI CCWWGG 3 cut(s) 192, 625, 648
FaeI CATG 4 cut(s) 34, 159, 535, 600
FatI CATG 4 cut(s) 30, 155, 531, 596
FauI CCCGC 1 cut(s) 563
Fnu4HI GCNGC 5 cut(s) 44, 353, 356, 713, 716
FokI GGATG 2 cut(s) 515, 608
FriOI GRGCYC 1 cut(s) 744
Fsp4HI GCNGC 5 cut(s) 44, 353, 356, 713, 716
FspBI CTAG 2 cut(s) 419, 763
GluI GCNGC 5 cut(s) 44, 353, 356, 713, 716
HaeIII GGCC 1 cut(s) 117
HapII CCGG 1 cut(s) 312
HgaI GACGC 2 cut(s) 671, 738
Hin1II CATG 4 cut(s) 34, 159, 535, 600
HindIII AAGCTT 2 cut(s) 133, 752
HinfI GANTC 2 cut(s) 139, 393
HpaII CCGG 1 cut(s) 312
HphI GGTGA 3 cut(s) 46, 416, 646
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 4 cut(s) 123, 376, 521, 657
Hpy188III TCNNGA 5 cut(s) 9, 182, 500, 616, 705
Hpy8I GTNNAC 1 cut(s) 424
HpyCH4III ACNGT 1 cut(s) 383
HpyCH4V TGCA 5 cut(s) 292, 358, 506, 600, 715
HpyF10VI GCNNNNNNNGC 5 cut(s) 85, 254, 361, 455, 671
HpyF3I CTNAG 6 cut(s) 120, 151, 498, 603, 654, 755
Hsp92II CATG 4 cut(s) 34, 159, 535, 600
Kzo9I GATC 1 cut(s) 24
LguI GCTCTTC 2 cut(s) 69, 749
LmnI GCTCC 2 cut(s) 409, 683
Lsp1109I GCAGC 5 cut(s) 30, 342, 364, 699, 727
LweI GCATC 2 cut(s) 453, 493
MaeI CTAG 2 cut(s) 419, 763
MaeIII GTNAC 1 cut(s) 34
MalI GATC 1 cut(s) 26
MboI GATC 1 cut(s) 24
MboII GAAGA 5 cut(s) 48, 86, 481, 579, 736
MhlI GDGCHC 1 cut(s) 744
MluCI AATT 7 cut(s) 12, 217, 306, 337, 398, 459, 735
MlyI GAGTC 2 cut(s) 148, 402
MnlI CCTC 7 cut(s) 38, 129, 189, 527, 603, 630, 729
MroXI GAANNNNTTC 1 cut(s) 539
MseI TTAA 4 cut(s) 230, 279, 387, 723
MslI CAYNNNNRTG 2 cut(s) 287, 297
MspA1I CMGCKG 3 cut(s) 355, 407, 712
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 1 cut(s) 428
Mva1269I GAATGC 2 cut(s) 259, 364
MvaI CCWGG 1 cut(s) 428
MwoI GCNNNNNNNGC 5 cut(s) 85, 254, 361, 455, 671
NdeII GATC 1 cut(s) 24
NlaIII CATG 4 cut(s) 34, 159, 535, 600
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 1 cut(s) 159
PaeR7I CTCGAG 1 cut(s) 703
PciSI GCTCTTC 2 cut(s) 69, 749
PctI GAATGC 2 cut(s) 259, 364
PdmI GAANNNNTTC 1 cut(s) 539
PkrI GCNGC 5 cut(s) 45, 354, 357, 714, 717
PleI GAGTC 2 cut(s) 147, 401
PpsI GAGTC 2 cut(s) 147, 401
PshBI ATTAAT 1 cut(s) 387
Psp124BI GAGCTC 1 cut(s) 744
Psp6I CCWGG 1 cut(s) 426
PspGI CCWGG 1 cut(s) 426
PspPI GGNCC 2 cut(s) 116, 184
PstI CTGCAG 1 cut(s) 717
PvuII CAGCTG 1 cut(s) 355
RsaI GTAC 1 cut(s) 641
RsaNI GTAC 1 cut(s) 640
RseI CAYNNNNRTG 2 cut(s) 287, 297
SacI GAGCTC 1 cut(s) 744
SapI GCTCTTC 2 cut(s) 69, 749
SaqAI TTAA 4 cut(s) 230, 279, 387, 723
SatI GCNGC 5 cut(s) 44, 353, 356, 713, 716
Sau3AI GATC 1 cut(s) 24
Sau96I GGNCC 2 cut(s) 116, 184
SchI GAGTC 2 cut(s) 148, 402
ScrFI CCNGG 1 cut(s) 428
SduI GDGCHC 1 cut(s) 744
SfaNI GCATC 2 cut(s) 453, 493
SfcI CTRYAG 1 cut(s) 713
Sfr274I CTCGAG 1 cut(s) 703
SinI GGWCC 1 cut(s) 184
SlaI CTCGAG 1 cut(s) 703
SmiMI CAYNNNNRTG 2 cut(s) 287, 297
SmlI CTYRAG 2 cut(s) 136, 703
SmoI CTYRAG 2 cut(s) 136, 703
Sse9I AATT 7 cut(s) 12, 217, 306, 337, 398, 459, 735
SsiI CCGC 3 cut(s) 407, 556, 710
SspI AATATT 1 cut(s) 563
SspMI CTAG 2 cut(s) 419, 763
SstI GAGCTC 1 cut(s) 744
StyD4I CCNGG 1 cut(s) 426
StyI CCWWGG 3 cut(s) 192, 625, 648
TaaI ACNGT 1 cut(s) 383
TaqI TCGA 4 cut(s) 23, 297, 543, 704
TasI AATT 7 cut(s) 12, 217, 306, 337, 398, 459, 735
Tru1I TTAA 4 cut(s) 230, 279, 387, 723
Tru9I TTAA 4 cut(s) 230, 279, 387, 723
TscAI CASTG 2 cut(s) 365, 607
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 5 cut(s) 43, 352, 355, 712, 715
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 3 cut(s) 404, 481, 548
TspRI CASTG 2 cut(s) 365, 607
VpaK11BI GGWCC 1 cut(s) 184
VspI ATTAAT 1 cut(s) 387
XagI CCTNNNNNAGG 2 cut(s) 191, 629
XapI RAATTY 2 cut(s) 12, 217
XceI RCATGY 1 cut(s) 159
XhoI CTCGAG 1 cut(s) 703
XmnI GAANNNNTTC 1 cut(s) 539
XspI CTAG 2 cut(s) 419, 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.