Rmu_sc0005561.1_g000003

Bifunctional monodehydroascorbate reductase and carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005561.1
OG0010395
Physical Location & Seq
Reverse (-)
9631 .. 13419
3789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005561.1_g000003.1.cds

Sequence Viewer

Length: 816 bp
atggcttcaagaatttcggtgtcgatcctcatggtgacattgctgcttgttggttcttcagcttgctttgtggaagagctgaagtcgcttggatttacttactcgggtgcgactggccctcagagatggggaagcttgagtccatatttctcagcatgtaatggaaagaagcaatctcctctggaccttgccaaggacaaggttattcgtagcaggaatttcaagccattaacccgaaattaccttgcgttgaatgccacattatacaacaatggctttaacattggggtgcatttcgatggacaaattgccggaacattagtcatagatggtaagaattacaacttgaagcagctgcactggcattctccctctgaacaccgtattaatggagtccaatttccagcggagcttcatctagttcacctggcagatgataatagcatcgcagttgtggcaaatcttttccaatatggcaaagatgatatcttcatctctcagatcaaggacaagctggcggagttggccgaggaggtatgtagaagagataaggatgcacatattcccctcggaaatatagacatgaaagagttcgataaaagaacccgcaaatattggagatatgttggctctctctcttctcctccttgcactgagaatgtcatctggaacatccttggaaaggtgaggtacatttccaaggctcagatagacgctataaaagcaccattgggagcagattacaagaacaactcaagaccgctgcagcctcttaatggacgcaaaattgagctcttccatgagcttagcaactag

Protein Analysis

271

Amino Acids

30.43

Weight (kDa)

9.24

Isoelectric Point (pI)

44.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52720 AT3G52720 AT3G52720 AT3G52720
fragaria_vesca FvH4_6g18830 FvH4_6g18830
malus_domestica MD12G1024600.v1.1
prunus_persica Prupe.7G109300_v2.0.a1 Prupe.7G109300_v2.0.a1
pyrus_communis pycom12g02430
rosa_chinensis RchiOBHm_Chr3g0473841
rosa_laevigata RLG00000023979
rosa_multiflora Rmu_sc0005561.1_g000003
rosa_roxburghii Rroxscaffold_6G00407730
rosa_rugosa Rorug03G0135900 Rorug03G0136000
rosa_samantha Rh3AG186400 Rh3BG215000 Rh3CG211700 Rh3DG210800
rosa_wichuraiana Rw3G017050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 407, 518, 607, 761
AclWI GGATC 1 cut(s) 19
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 2 cut(s) 12, 217
AcuI CTGAAG 2 cut(s) 42, 101
AfaI GTAC 1 cut(s) 692
AfiI CCNNNNNNNGG 3 cut(s) 193, 682, 776
AgsI TTSAA 4 cut(s) 9, 223, 253, 349
AjnI CCWGG 1 cut(s) 426
AluBI AGCT 8 cut(s) 62, 79, 135, 355, 412, 514, 793, 805
AluI AGCT 8 cut(s) 62, 79, 135, 355, 412, 514, 793, 805
Alw21I GWGCWC 1 cut(s) 795
AlwI GGATC 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 103
AoxI GGCC 2 cut(s) 115, 525
ApeKI GCWGC 5 cut(s) 43, 352, 355, 763, 766
ApoI RAATTY 2 cut(s) 12, 217
AseI ATTAAT 1 cut(s) 387
Asp700I GAANNNNTTC 1 cut(s) 590
AspS9I GGNCC 2 cut(s) 116, 184
AsuHPI GGTGA 3 cut(s) 46, 416, 697
AvaI CYCGRG 1 cut(s) 103
AvaII GGWCC 1 cut(s) 184
BanII GRGCYC 1 cut(s) 795
Bbv12I GWGCWC 1 cut(s) 795
BbvI GCAGC 5 cut(s) 30, 342, 364, 750, 778
BccI CCATC 3 cut(s) 120, 293, 323
BciT130I CCWGG 1 cut(s) 428
BfaI CTAG 2 cut(s) 419, 814
BfmI CTRYAG 1 cut(s) 764
BisI GCNGC 5 cut(s) 44, 353, 356, 764, 767
BlpI GCTNAGC 1 cut(s) 806
BlsI GCNGC 5 cut(s) 45, 354, 357, 765, 768
Bme1390I CCNGG 1 cut(s) 428
Bme18I GGWCC 1 cut(s) 184
BmeT110I CYCGRG 1 cut(s) 103
BmgT120I GGNCC 2 cut(s) 116, 184
BmrFI CCNGG 1 cut(s) 428
BmsI GCATC 2 cut(s) 453, 544
Bpu1102I GCTNAGC 1 cut(s) 806
BpuEI CTTGAG 2 cut(s) 157, 739
BsaJI CCNNGG 5 cut(s) 192, 528, 568, 676, 699
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 682, 776
Bse1I ACTGG 2 cut(s) 118, 365
Bse3DI GCAATG 1 cut(s) 38
BseBI CCWGG 1 cut(s) 428
BseDI CCNNGG 5 cut(s) 192, 528, 568, 676, 699
BseGI GGATG 2 cut(s) 559, 672
BseLI CCNNNNNNNGG 3 cut(s) 193, 682, 776
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 5 cut(s) 134, 165, 512, 645, 719
BseNI ACTGG 2 cut(s) 118, 365
BseRI GAGGAG 3 cut(s) 168, 545, 633
BseXI GCAGC 5 cut(s) 30, 342, 364, 750, 778
BsgI GTGCAG 1 cut(s) 341
BshFI GGCC 2 cut(s) 117, 527
BsiHKAI GWGCWC 1 cut(s) 795
BsiHKCI CYCGRG 1 cut(s) 103
BsiSI CCGG 1 cut(s) 312
BslI CCNNNNNNNGG 3 cut(s) 193, 682, 776
BsmI GAATGC 2 cut(s) 259, 364
BsnI GGCC 2 cut(s) 117, 527
BsoBI CYCGRG 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 795
Bsp143I GATC 2 cut(s) 24, 501
Bsp1720I GCTNAGC 1 cut(s) 806
BspACI CCGC 4 cut(s) 407, 518, 607, 761
BspANI GGCC 2 cut(s) 117, 527
BspCNI CTCAG 5 cut(s) 133, 164, 511, 646, 718
BspMAI CTGCAG 1 cut(s) 768
BspPI GGATC 1 cut(s) 19
BspQI GCTCTTC 2 cut(s) 69, 800
BsrDI GCAATG 1 cut(s) 38
BsrI ACTGG 2 cut(s) 118, 365
BssECI CCNNGG 5 cut(s) 192, 528, 568, 676, 699
BssMI GATC 2 cut(s) 24, 501
BssT1I CCWWGG 3 cut(s) 192, 676, 699
Bst2UI CCWGG 1 cut(s) 428
Bst4CI ACNGT 1 cut(s) 383
Bst6I CTCTTC 4 cut(s) 69, 538, 643, 800
BstC8I GCNNGC 2 cut(s) 64, 516
BstDEI CTNAG 6 cut(s) 120, 151, 498, 654, 705, 806
BstENI CCTNNNNNAGG 2 cut(s) 191, 680
BstF5I GGATG 2 cut(s) 559, 672
BstKTI GATC 2 cut(s) 27, 504
BstMBI GATC 2 cut(s) 24, 501
BstMWI GCNNNNNNNGC 6 cut(s) 85, 254, 361, 455, 524, 722
BstNI CCWGG 1 cut(s) 428
BstNSI RCATGY 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 426
BstSFI CTRYAG 1 cut(s) 764
BstV1I GCAGC 5 cut(s) 30, 342, 364, 750, 778
BsuRI GGCC 2 cut(s) 117, 527
BtgZI GCGATG 1 cut(s) 430
BtsCI GGATG 2 cut(s) 559, 672
BtsIMutI CAGTG 2 cut(s) 358, 651
Cac8I GCNNGC 2 cut(s) 64, 516
Cfr13I GGNCC 2 cut(s) 116, 184
CseI GACGC 2 cut(s) 722, 789
Csp6I GTAC 1 cut(s) 691
CviAII CATG 4 cut(s) 31, 156, 583, 800
CviQI GTAC 1 cut(s) 691
DdeI CTNAG 6 cut(s) 120, 151, 498, 654, 705, 806
DpnI GATC 2 cut(s) 26, 503
DpnII GATC 2 cut(s) 24, 501
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 4 cut(s) 69, 538, 643, 800
EarI CTCTTC 4 cut(s) 69, 538, 643, 800
EciI GGCGGA 1 cut(s) 533
Ecl136II GAGCTC 1 cut(s) 793
Eco130I CCWWGG 3 cut(s) 192, 676, 699
Eco24I GRGCYC 1 cut(s) 795
Eco32I GATATC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 184
Eco53kI GAGCTC 1 cut(s) 793
Eco57I CTGAAG 2 cut(s) 42, 101
Eco88I CYCGRG 1 cut(s) 103
EcoICRI GAGCTC 1 cut(s) 793
EcoNI CCTNNNNNAGG 2 cut(s) 191, 680
EcoRII CCWGG 1 cut(s) 426
EcoRV GATATC 1 cut(s) 487
EcoT14I CCWWGG 3 cut(s) 192, 676, 699
EcoT38I GRGCYC 1 cut(s) 795
ErhI CCWWGG 3 cut(s) 192, 676, 699
FaeI CATG 4 cut(s) 34, 159, 586, 803
FatI CATG 4 cut(s) 30, 155, 582, 799
FauI CCCGC 1 cut(s) 614
Fnu4HI GCNGC 5 cut(s) 44, 353, 356, 764, 767
FokI GGATG 2 cut(s) 566, 659
FriOI GRGCYC 1 cut(s) 795
Fsp4HI GCNGC 5 cut(s) 44, 353, 356, 764, 767
FspBI CTAG 2 cut(s) 419, 814
GluI GCNGC 5 cut(s) 44, 353, 356, 764, 767
HaeIII GGCC 2 cut(s) 117, 527
HapII CCGG 1 cut(s) 312
HgaI GACGC 2 cut(s) 722, 789
Hin1II CATG 4 cut(s) 34, 159, 586, 803
HindIII AAGCTT 1 cut(s) 133
HinfI GANTC 2 cut(s) 139, 393
HpaII CCGG 1 cut(s) 312
HphI GGTGA 3 cut(s) 46, 416, 697
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 5 cut(s) 123, 376, 501, 572, 708
Hpy188III TCNNGA 4 cut(s) 9, 182, 667, 756
Hpy8I GTNNAC 1 cut(s) 424
HpyCH4III ACNGT 1 cut(s) 383
HpyCH4V TGCA 5 cut(s) 292, 358, 557, 651, 766
HpyF10VI GCNNNNNNNGC 6 cut(s) 85, 254, 361, 455, 524, 722
HpyF3I CTNAG 6 cut(s) 120, 151, 498, 654, 705, 806
Hsp92II CATG 4 cut(s) 34, 159, 586, 803
Kzo9I GATC 2 cut(s) 24, 501
LguI GCTCTTC 2 cut(s) 69, 800
LmnI GCTCC 2 cut(s) 409, 734
Lsp1109I GCAGC 5 cut(s) 30, 342, 364, 750, 778
LweI GCATC 2 cut(s) 453, 544
MaeI CTAG 2 cut(s) 419, 814
MaeIII GTNAC 1 cut(s) 34
MalI GATC 2 cut(s) 26, 503
MboI GATC 2 cut(s) 24, 501
MboII GAAGA 6 cut(s) 48, 86, 481, 555, 630, 787
MhlI GDGCHC 1 cut(s) 795
MluCI AATT 7 cut(s) 12, 217, 238, 306, 337, 398, 786
MlyI GAGTC 2 cut(s) 148, 402
MroXI GAANNNNTTC 1 cut(s) 590
MseI TTAA 4 cut(s) 230, 279, 387, 774
MslI CAYNNNNRTG 2 cut(s) 287, 297
MspA1I CMGCKG 3 cut(s) 355, 407, 763
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 1 cut(s) 428
Mva1269I GAATGC 2 cut(s) 259, 364
MvaI CCWGG 1 cut(s) 428
MwoI GCNNNNNNNGC 6 cut(s) 85, 254, 361, 455, 524, 722
NdeII GATC 2 cut(s) 24, 501
NlaIII CATG 4 cut(s) 34, 159, 586, 803
NmeAIII GCCGAG 1 cut(s) 553
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 1 cut(s) 159
PciSI GCTCTTC 2 cut(s) 69, 800
PctI GAATGC 2 cut(s) 259, 364
PdmI GAANNNNTTC 1 cut(s) 590
PkrI GCNGC 5 cut(s) 45, 354, 357, 765, 768
PleI GAGTC 2 cut(s) 147, 401
PpsI GAGTC 2 cut(s) 147, 401
PshBI ATTAAT 1 cut(s) 387
Psp124BI GAGCTC 1 cut(s) 795
Psp6I CCWGG 1 cut(s) 426
PspGI CCWGG 1 cut(s) 426
PspPI GGNCC 2 cut(s) 116, 184
PstI CTGCAG 1 cut(s) 768
PvuII CAGCTG 1 cut(s) 355
RsaI GTAC 1 cut(s) 692
RsaNI GTAC 1 cut(s) 691
RseI CAYNNNNRTG 2 cut(s) 287, 297
SacI GAGCTC 1 cut(s) 795
SapI GCTCTTC 2 cut(s) 69, 800
SaqAI TTAA 4 cut(s) 230, 279, 387, 774
SatI GCNGC 5 cut(s) 44, 353, 356, 764, 767
Sau3AI GATC 2 cut(s) 24, 501
Sau96I GGNCC 2 cut(s) 116, 184
SchI GAGTC 2 cut(s) 148, 402
ScrFI CCNGG 1 cut(s) 428
SduI GDGCHC 1 cut(s) 795
SfaNI GCATC 2 cut(s) 453, 544
SfcI CTRYAG 1 cut(s) 764
SinI GGWCC 1 cut(s) 184
SmiMI CAYNNNNRTG 2 cut(s) 287, 297
SmlI CTYRAG 2 cut(s) 136, 754
SmoI CTYRAG 2 cut(s) 136, 754
Sse9I AATT 7 cut(s) 12, 217, 238, 306, 337, 398, 786
SsiI CCGC 4 cut(s) 407, 518, 607, 761
SspI AATATT 1 cut(s) 614
SspMI CTAG 2 cut(s) 419, 814
SstI GAGCTC 1 cut(s) 795
StyD4I CCNGG 1 cut(s) 426
StyI CCWWGG 3 cut(s) 192, 676, 699
TaaI ACNGT 1 cut(s) 383
TaqI TCGA 3 cut(s) 23, 297, 594
TasI AATT 7 cut(s) 12, 217, 238, 306, 337, 398, 786
Tru1I TTAA 4 cut(s) 230, 279, 387, 774
Tru9I TTAA 4 cut(s) 230, 279, 387, 774
TscAI CASTG 2 cut(s) 365, 658
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 5 cut(s) 43, 352, 355, 763, 766
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 3 cut(s) 404, 481, 599
TspRI CASTG 2 cut(s) 365, 658
VpaK11BI GGWCC 1 cut(s) 184
VspI ATTAAT 1 cut(s) 387
XagI CCTNNNNNAGG 2 cut(s) 191, 680
XapI RAATTY 2 cut(s) 12, 217
XceI RCATGY 1 cut(s) 159
XmnI GAANNNNTTC 1 cut(s) 590
XspI CTAG 2 cut(s) 419, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.