MD12G1024600.v1.1

Bifunctional monodehydroascorbate reductase and carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010395
Physical Location & Seq
Forward (+)
2641134 .. 2648116
6983 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1024600.v1.1.491

Sequence Viewer

Length: 813 bp
ATGGCCCTTAGAGTTTCCTTCTCCTTCCTTGTGATAGCTTTGCTGCTCGTCTTCGGCCCTTCGGCTTCTGTCCAAGATGACTCACTCTCATTTAGTTACACTGGTCAAACTGGCCCTGAGAAATGGGGAAGCTTGAGTCCAAATAACACAGCATGCTCAAATGGGAAGATGCAGTCTCCGGTGGACATCGTCAAGAGCATACTTACCCCTAACAAGAAACTAATGCCGTTGACCAGAGTCTACCGTACTTCAAATGCCACATTGTTTAACAACGGCTTTAACCTTGGGTTGCGTTTCGAAGGAAATGGGGGAACATTGGATGTGGATGGTAAGAACTACAACTTGATGCAGTTGCACTGGCACACTCCCTCTGAGCATCGCCTTAATGGAATCCAATTTCCAGCGGAGCTTCATCTGCTTCACCAGGCAGCTGATCAAAGCCGCGCAGTTGTGGCAATCCTCTTCAATTTCGGCAAGGAAGATATCATCCTCTTACAGATTAAGAACAGGCTGGCCGAGCTGGCCAAGGAGGCGTGCAAGAAAGATGAAGATGCTACCATTCCCGTTGGAACTGTTGACCTGAACGAGTTACAGAAGAAGAGTCGCAAATATTACAAATACGTTGGCTCTCTCACCGTTCCTCCATGCACCGAAAATGTTGTCTGGAGCATTCTCGGAAAGGTGAGGACTATTTCCAAGCATCAGTTAGATGCACTAAAAGCACCATTGGATGCATCTTGCAAAAACAACTCAAGACCTCTTCAATCTCCAAATGGACGCAAGATTGAGCTCTACGATGAGCTTATGCACTAG

Protein Analysis

271

Amino Acids

30.0

Weight (kDa)

9.07

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 38 - 263 4.7e-38 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52720 AT3G52720 AT3G52720 AT3G52720
fragaria_vesca FvH4_6g18830 FvH4_6g18830
malus_domestica MD12G1024600.v1.1
prunus_persica Prupe.7G109300_v2.0.a1 Prupe.7G109300_v2.0.a1
pyrus_communis pycom12g02430
rosa_chinensis RchiOBHm_Chr3g0473841
rosa_laevigata RLG00000023979
rosa_multiflora Rmu_sc0005561.1_g000003
rosa_roxburghii Rroxscaffold_6G00407730
rosa_rugosa Rorug03G0135900 Rorug03G0136000
rosa_samantha Rh3AG186400 Rh3BG215000 Rh3CG211700 Rh3DG210800
rosa_wichuraiana Rw3G017050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 240
AccII CGCG 1 cut(s) 444
AciI CCGC 2 cut(s) 404, 442
AcoI YGGCCR 2 cut(s) 513, 522
AfaI GTAC 1 cut(s) 247
AgsI TTSAA 3 cut(s) 252, 466, 764
AjnI CCWGG 1 cut(s) 423
AluBI AGCT 7 cut(s) 38, 132, 409, 431, 520, 790, 802
AluI AGCT 7 cut(s) 38, 132, 409, 431, 520, 790, 802
Alw21I GWGCWC 1 cut(s) 792
Alw26I GTCTC 1 cut(s) 180
AoxI GGCC 5 cut(s) 3, 55, 112, 513, 522
ApeKI GCWGC 2 cut(s) 43, 428
ArsI GACNNNNNNTTYG 2 cut(s) 645, 677
AspLEI GCGC 1 cut(s) 446
AspS9I GGNCC 3 cut(s) 4, 56, 113
AsuHPI GGTGA 3 cut(s) 413, 625, 694
AsuII TTCGAA 1 cut(s) 297
BalI TGGCCA 1 cut(s) 524
BanII GRGCYC 1 cut(s) 792
BbsI GAAGAC 1 cut(s) 43
Bbv12I GWGCWC 1 cut(s) 792
BbvI GCAGC 2 cut(s) 30, 440
BccI CCATC 1 cut(s) 320
BceAI ACGGC 2 cut(s) 211, 289
BciT130I CCWGG 1 cut(s) 425
BclI TGATCA 1 cut(s) 433
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 1 cut(s) 811
BglI GCCNNNNNGGC 2 cut(s) 521, 530
BisI GCNGC 3 cut(s) 44, 429, 442
BlsI GCNGC 3 cut(s) 45, 430, 443
Bme1390I CCNGG 1 cut(s) 425
BmgT120I GGNCC 3 cut(s) 4, 56, 113
BmrFI CCNGG 1 cut(s) 425
BmsI GCATC 8 cut(s) 159, 336, 385, 541, 700, 709, 721, 743
BoxI GACNNNNGTC 1 cut(s) 236
BpiI GAAGAC 1 cut(s) 43
BpmI CTGGAG 1 cut(s) 685
Bpu14I TTCGAA 1 cut(s) 297
BpuEI CTTGAG 2 cut(s) 154, 736
BsaJI CCNNGG 2 cut(s) 283, 525
BsaWI WCCGGW 1 cut(s) 178
BsaXI ACNNNNNCTCC 2 cut(s) 625, 655
Bse1I ACTGG 3 cut(s) 106, 115, 362
BseBI CCWGG 1 cut(s) 425
BseDI CCNNGG 2 cut(s) 283, 525
BseGI GGATG 4 cut(s) 325, 331, 486, 736
BseMII CTCAG 2 cut(s) 108, 363
BseNI ACTGG 3 cut(s) 106, 115, 362
BseXI GCAGC 2 cut(s) 30, 440
Bsh1236I CGCG 1 cut(s) 444
BshFI GGCC 5 cut(s) 5, 57, 114, 515, 524
BsiHKAI GWGCWC 1 cut(s) 792
BsiSI CCGG 1 cut(s) 179
BsmAI GTCTC 1 cut(s) 180
BsmI GAATGC 1 cut(s) 669
BsnI GGCC 5 cut(s) 5, 57, 114, 515, 524
Bsp119I TTCGAA 1 cut(s) 297
Bsp1286I GDGCHC 1 cut(s) 792
Bsp143I GATC 1 cut(s) 433
BspACI CCGC 2 cut(s) 404, 442
BspANI GGCC 5 cut(s) 5, 57, 114, 515, 524
BspCNI CTCAG 2 cut(s) 109, 364
BspFNI CGCG 1 cut(s) 444
BspT104I TTCGAA 1 cut(s) 297
BsrI ACTGG 3 cut(s) 106, 115, 362
BssECI CCNNGG 2 cut(s) 283, 525
BssMI GATC 1 cut(s) 433
BssT1I CCWWGG 2 cut(s) 283, 525
Bst2UI CCWGG 1 cut(s) 425
Bst4CI ACNGT 3 cut(s) 245, 574, 637
Bst6I CTCTTC 3 cut(s) 467, 593, 765
BstBI TTCGAA 1 cut(s) 297
BstC8I GCNNGC 4 cut(s) 154, 513, 522, 535
BstDEI CTNAG 3 cut(s) 8, 117, 372
BstF5I GGATG 4 cut(s) 325, 331, 486, 736
BstFNI CGCG 1 cut(s) 444
BstHHI GCGC 1 cut(s) 446
BstKTI GATC 1 cut(s) 436
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 1 cut(s) 433
BstMWI GCNNNNNNNGC 6 cut(s) 415, 452, 517, 521, 530, 719
BstNI CCWGG 1 cut(s) 425
BstNSI RCATGY 1 cut(s) 156
BstPAI GACNNNNGTC 1 cut(s) 236
BstSCI CCNGG 1 cut(s) 423
BstUI CGCG 1 cut(s) 444
BstV1I GCAGC 2 cut(s) 30, 440
BstV2I GAAGAC 1 cut(s) 43
BsuRI GGCC 5 cut(s) 5, 57, 114, 515, 524
BtgZI GCGATG 1 cut(s) 362
BtsCI GGATG 4 cut(s) 325, 331, 486, 736
BtsIMutI CAGTG 2 cut(s) 99, 355
Cac8I GCNNGC 4 cut(s) 154, 513, 522, 535
CfoI GCGC 1 cut(s) 446
Cfr13I GGNCC 3 cut(s) 4, 56, 113
CseI GACGC 1 cut(s) 786
Csp6I GTAC 1 cut(s) 246
CviAII CATG 2 cut(s) 153, 645
CviQI GTAC 1 cut(s) 246
DdeI CTNAG 3 cut(s) 8, 117, 372
DpnI GATC 1 cut(s) 435
DpnII GATC 1 cut(s) 433
EaeI YGGCCR 2 cut(s) 513, 522
Eam1104I CTCTTC 3 cut(s) 467, 593, 765
EarI CTCTTC 3 cut(s) 467, 593, 765
Ecl136II GAGCTC 1 cut(s) 790
Eco130I CCWWGG 2 cut(s) 283, 525
Eco24I GRGCYC 1 cut(s) 792
Eco32I GATATC 1 cut(s) 484
Eco53kI GAGCTC 1 cut(s) 790
EcoICRI GAGCTC 1 cut(s) 790
EcoRII CCWGG 1 cut(s) 423
EcoRV GATATC 1 cut(s) 484
EcoT14I CCWWGG 2 cut(s) 283, 525
EcoT22I ATGCAT 1 cut(s) 736
EcoT38I GRGCYC 1 cut(s) 792
ErhI CCWWGG 2 cut(s) 283, 525
FaeI CATG 2 cut(s) 156, 648
FaiI YATR 4 cut(s) 154, 200, 646, 806
FatI CATG 2 cut(s) 152, 644
FbaI TGATCA 1 cut(s) 433
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 3 cut(s) 44, 429, 442
FokI GGATG 4 cut(s) 332, 338, 473, 743
FriOI GRGCYC 1 cut(s) 792
Fsp4HI GCNGC 3 cut(s) 44, 429, 442
FspBI CTAG 1 cut(s) 811
GlaI GCGC 1 cut(s) 445
GluI GCNGC 3 cut(s) 44, 429, 442
GsuI CTGGAG 1 cut(s) 685
HaeIII GGCC 5 cut(s) 5, 57, 114, 515, 524
HapII CCGG 1 cut(s) 179
HgaI GACGC 1 cut(s) 786
HhaI GCGC 1 cut(s) 446
Hin1II CATG 2 cut(s) 156, 648
Hin6I GCGC 1 cut(s) 444
HinP1I GCGC 1 cut(s) 444
HincII GTYRAC 2 cut(s) 231, 577
HindII GTYRAC 2 cut(s) 231, 577
HindIII AAGCTT 1 cut(s) 130
HinfI GANTC 5 cut(s) 80, 136, 237, 390, 601
HpaII CCGG 1 cut(s) 179
HphI GGTGA 3 cut(s) 413, 625, 694
Hpy166II GTNNAC 4 cut(s) 184, 231, 241, 577
Hpy188I TCNGA 2 cut(s) 373, 677
Hpy188III TCNNGA 3 cut(s) 193, 664, 753
Hpy8I GTNNAC 4 cut(s) 184, 231, 241, 577
HpyAV CCTTC 4 cut(s) 28, 34, 69, 293
HpyCH4III ACNGT 3 cut(s) 245, 574, 637
HpyCH4IV ACGT 1 cut(s) 621
HpyCH4V TGCA 9 cut(s) 172, 349, 355, 537, 648, 713, 734, 741, 808
HpyF10VI GCNNNNNNNGC 6 cut(s) 415, 452, 517, 521, 530, 719
HpyF3I CTNAG 3 cut(s) 8, 117, 372
HpySE526I ACGT 1 cut(s) 621
Hsp92II CATG 2 cut(s) 156, 648
HspAI GCGC 1 cut(s) 444
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 1 cut(s) 433
LmnI GCTCC 2 cut(s) 406, 666
Lsp1109I GCAGC 2 cut(s) 30, 440
LweI GCATC 8 cut(s) 159, 336, 385, 541, 700, 709, 721, 743
MaeI CTAG 1 cut(s) 811
MaeII ACGT 1 cut(s) 621
MaeIII GTNAC 2 cut(s) 95, 588
MalI GATC 1 cut(s) 435
MboI GATC 1 cut(s) 433
MboII GAAGA 8 cut(s) 43, 178, 454, 491, 560, 607, 610, 752
MhlI GDGCHC 1 cut(s) 792
MlsI TGGCCA 1 cut(s) 524
MluCI AATT 2 cut(s) 395, 466
MluNI TGGCCA 1 cut(s) 524
MlyI GAGTC 4 cut(s) 74, 145, 246, 610
MmeI TCCRAC 1 cut(s) 547
MnlI CCTC 7 cut(s) 379, 470, 500, 523, 651, 678, 768
Mox20I TGGCCA 1 cut(s) 524
Mph1103I ATGCAT 1 cut(s) 736
MscI TGGCCA 1 cut(s) 524
MseI TTAA 4 cut(s) 267, 279, 384, 501
Msp20I TGGCCA 1 cut(s) 524
MspA1I CMGCKG 2 cut(s) 404, 431
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 1 cut(s) 425
Mva1269I GAATGC 1 cut(s) 669
MvaI CCWGG 1 cut(s) 425
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 6 cut(s) 415, 452, 517, 521, 530, 719
NdeII GATC 1 cut(s) 433
NlaIII CATG 2 cut(s) 156, 648
NmeAIII GCCGAG 1 cut(s) 541
NsiI ATGCAT 1 cut(s) 736
NspI RCATGY 1 cut(s) 156
NspV TTCGAA 1 cut(s) 297
PaeI GCATGC 1 cut(s) 156
PctI GAATGC 1 cut(s) 669
PfeI GAWTC 1 cut(s) 390
PflFI GACNNNGTC 1 cut(s) 188
PkrI GCNGC 3 cut(s) 45, 430, 443
PleI GAGTC 4 cut(s) 74, 144, 245, 609
PpsI GAGTC 4 cut(s) 74, 144, 245, 609
PshAI GACNNNNGTC 1 cut(s) 236
Psp124BI GAGCTC 1 cut(s) 792
Psp6I CCWGG 1 cut(s) 423
PspGI CCWGG 1 cut(s) 423
PspPI GGNCC 3 cut(s) 4, 56, 113
PsyI GACNNNGTC 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 1 cut(s) 247
RsaNI GTAC 1 cut(s) 246
SacI GAGCTC 1 cut(s) 792
SaqAI TTAA 4 cut(s) 267, 279, 384, 501
SatI GCNGC 3 cut(s) 44, 429, 442
Sau3AI GATC 1 cut(s) 433
Sau96I GGNCC 3 cut(s) 4, 56, 113
SchI GAGTC 4 cut(s) 74, 145, 246, 610
ScrFI CCNGG 1 cut(s) 425
SduI GDGCHC 1 cut(s) 792
SfaNI GCATC 8 cut(s) 159, 336, 385, 541, 700, 709, 721, 743
SfiI GGCCNNNNNGGCC 1 cut(s) 521
SfuI TTCGAA 1 cut(s) 297
SmlI CTYRAG 2 cut(s) 133, 751
SmoI CTYRAG 2 cut(s) 133, 751
SphI GCATGC 1 cut(s) 156
Sse9I AATT 2 cut(s) 395, 466
SsiI CCGC 2 cut(s) 404, 442
SspI AATATT 1 cut(s) 611
SspMI CTAG 1 cut(s) 811
SstI GAGCTC 1 cut(s) 792
StyD4I CCNGG 1 cut(s) 423
StyI CCWWGG 2 cut(s) 283, 525
TaaI ACNGT 3 cut(s) 245, 574, 637
TaiI ACGT 1 cut(s) 624
TaqI TCGA 1 cut(s) 297
TasI AATT 2 cut(s) 395, 466
TauI GCSGC 1 cut(s) 444
TfiI GAWTC 1 cut(s) 390
Tru1I TTAA 4 cut(s) 267, 279, 384, 501
Tru9I TTAA 4 cut(s) 267, 279, 384, 501
TscAI CASTG 2 cut(s) 106, 362
TseI GCWGC 2 cut(s) 43, 428
TspDTI ATGAA 2 cut(s) 401, 561
TspRI CASTG 2 cut(s) 106, 362
Tth111I GACNNNGTC 1 cut(s) 188
XceI RCATGY 1 cut(s) 156
XmiI GTMKAC 1 cut(s) 240
XspI CTAG 1 cut(s) 811
Zsp2I ATGCAT 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.