AT3G53170

Pentatricopeptide repeat-containing protein At3g53170

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004347
Physical Location & Seq
Reverse (-)
19704106 .. 19706536
2431 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G53170.1

Sequence Viewer

Length: 1500 bp
ATGGCATTGATACAAAATCCTGTTCAAGGAACTACTTCCGCGTACGCCAATGAGATCGCACAACTTGGTTTTTCTCGAAGCGTGGTTCAGCAACATATATCTTCCCCTGTTTATTTTCGGTGTATACCAACTATTTCAATAACGCCCACAATGTGTTCGACAAAGGTCCCCAACGAACGTACAGAGAAAATGAATTCTGGTTTGATCTCAACAAGACACCAAGTAGATCCTAAGAAAGAATTGTCTCGGATTCTGAGAACAGATGCTGCTGTGAAGGGCATTGAGAGGAAAGCCAATTCCGAAAAGTACTTAACTTTATGGCCAAAAGCTGTTCTAGAAGCTCTTGACGAAGCTATCAAAGAGAATCGATGGCAGTCTGCTCTCAAGATTTTCAATCTTCTTAGGAAGCAGCATTGGTATGAACCAAGATGCAAAACTTATACAAAGCTGTTTAAAGTGCTTGGGAATTGTAAACAGCCTGATCAAGCCAGCTTGCTTTTCGAAGTTATGTTATCTGAAGGATTGAAACCAACCATTGATGTGTATACCTCTCTTATCTCTGTTTATGGCAAAAGCGAGCTTCTAGACAAGGCGTTTTCTACACTTGAGTATATGAAATCTGTTAGTGACTGCAAGCCAGATGTGTTCACCTTTACTGTTCTCATCAGTTGTTGCTGCAAACTGGGTCGGTTTGATCTGGTTAAAAGTATCGTTCTTGAGATGTCGTATCTTGGAGTTGGATGCAGCACGGTTACTTACAATACTATTATTGATGGGTATGGAAAGGCTGGAATGTTTGAGGAAATGGAAAGTGTTCTGGCTGATATGATCGAGGACGGAGATTCTCTTCCGGATGTTTGTACGCTTAACTCGATCATTGGGTCATATGGAAATGGTCGCAACATGAGGAAGATGGAGAGCTGGTATAGTCGGTTCCAGCTGATGGGGGTGCAACCGGATATCACTACATTTAACATACTGATCCTGTCATTTGGAAAAGCTGGTATGTATAAGAAGATGTGCTCTGTTATGGATTTTATGGAGAAAAGGTTCTTCTCCCTGACGACTGTCACTTACAATATAGTGATTGAGACGTTTGGAAAAGCTGGAAGAATTGAGAAAATGGATGATGTATTCAGGAAAATGAAGTATCAAGGAGTGAAACCAAACTCAATCACTTATTGTTCGCTTGTTAACGCGTATAGCAAAGCAGGTCTTGTCGTTAAGATAGATTCGGTTTTGAGGCAAATTGTGAATTCAGATGTGGTACTGGATACACCGTTCTTCAATTGCATAATCAATGCATATGGTCAGGCTGGTGATTTGGCTACAATGAAAGAATTATACATACAAATGGAAGAGAGAAAATGTAAGCCTGATAAAATCACTTTTGCTACTATGATCAAAACCTATACTGCTCATGGGATATTCGATGCAGTCCAGGAACTCGAGAAACAAATGATTTCCTCTGATATAGGCAAGAAGAGGCTTACGGAGTGA

Protein Analysis

499

Amino Acids

56.4

Weight (kDa)

8.72

Isoelectric Point (pI)

35.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 177 - 227 4.1e-12 PPR repeat family
PPR_1 PF12854 210 - 232 4.4e-06 PPR repeat
PPR_3 PF13812 236 - 298 5.2e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 249 - 280 4.3e-06 PPR repeat family
PPR PF01535 251 - 280 1.1e-08 PPR repeat
PPR_2 PF13041 284 - 330 7e-07 PPR repeat family
PPR_2 PF13041 319 - 363 6.9e-07 PPR repeat family
TPR_24 PF23276 333 - 450 6e-10 Fungal tetratrico peptide repeats
PPR_2 PF13041 355 - 403 1.1e-13 PPR repeat family
PPR PF01535 357 - 387 8.8e-06 PPR repeat
PPR_3 PF13812 412 - 471 1.1e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 426 - 470 4.9e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1202
AccB7I CCANNNNNTGG 1 cut(s) 943
AccI GTMKAC 2 cut(s) 124, 545
AccII CGCG 2 cut(s) 41, 1199
AccIII TCCGGA 1 cut(s) 850
AciI CCGC 1 cut(s) 39
AclWI GGATC 2 cut(s) 221, 976
AcoI YGGCCR 1 cut(s) 320
AcsI RAATTY 2 cut(s) 193, 1255
AcuI CTGAAG 1 cut(s) 537
AdeI CACNNNGTG 1 cut(s) 153
AfaI GTAC 5 cut(s) 44, 181, 308, 862, 1269
AfiI CCNNNNNNNGG 2 cut(s) 26, 943
AflIII ACRYGT 1 cut(s) 1197
AgsI TTSAA 5 cut(s) 26, 138, 394, 526, 1288
AjnI CCWGG 1 cut(s) 1440
AloI GAACNNNNNNTCC 4 cut(s) 1034, 1066, 1265, 1297
Alw21I GWGCWC 1 cut(s) 1025
Alw26I GTCTC 2 cut(s) 249, 1085
AlwI GGATC 2 cut(s) 221, 976
AlwNI CAGNNNCTG 1 cut(s) 266
Ama87I CYCGRG 1 cut(s) 1448
Aor13HI TCCGGA 1 cut(s) 850
AoxI GGCC 1 cut(s) 320
ApeKI GCWGC 4 cut(s) 266, 409, 675, 744
ApoI RAATTY 2 cut(s) 193, 1255
Asp700I GAANNNNTTC 3 cut(s) 34, 813, 1049
AspS9I GGNCC 1 cut(s) 166
AsuHPI GGTGA 2 cut(s) 640, 1331
AsuII TTCGAA 1 cut(s) 501
AvaI CYCGRG 1 cut(s) 1448
AvaII GGWCC 1 cut(s) 166
BalI TGGCCA 1 cut(s) 322
Bbv12I GWGCWC 1 cut(s) 1025
BbvI GCAGC 4 cut(s) 253, 421, 662, 756
BccI CCATC 4 cut(s) 363, 767, 907, 937
BciT130I CCWGG 1 cut(s) 1442
BciVI GTATCC 1 cut(s) 1267
BclI TGATCA 2 cut(s) 481, 1401
BcoDI GTCTC 2 cut(s) 249, 1085
BfaI CTAG 2 cut(s) 335, 584
BfuAI ACCTGC 1 cut(s) 1202
BfuI GTATCC 1 cut(s) 1267
BisI GCNGC 4 cut(s) 267, 410, 676, 745
BlsI GCNGC 4 cut(s) 268, 411, 677, 746
BmcAI AGTACT 1 cut(s) 308
Bme1390I CCNGG 1 cut(s) 1442
Bme18I GGWCC 1 cut(s) 166
BmeT110I CYCGRG 1 cut(s) 1448
BmgT120I GGNCC 1 cut(s) 166
BmiI GGNNCC 2 cut(s) 168, 935
BmrFI CCNGG 1 cut(s) 1442
BmrI ACTGGG 1 cut(s) 692
BmsI GCATC 4 cut(s) 253, 419, 731, 1423
BmuI ACTGGG 1 cut(s) 692
BoxI GACNNNNGTC 2 cut(s) 164, 1067
Bpu14I TTCGAA 1 cut(s) 501
BpuEI CTTGAG 3 cut(s) 368, 626, 737
Bsa29I ATCGAT 1 cut(s) 367
BsaWI WCCGGW 2 cut(s) 850, 955
BsaXI ACNNNNNCTCC 4 cut(s) 908, 938, 1034, 1064
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 943
Bse1I ACTGG 2 cut(s) 687, 1275
BseAI TCCGGA 1 cut(s) 850
BseBI CCWGG 1 cut(s) 1442
BseCI ATCGAT 1 cut(s) 367
BseGI GGATG 3 cut(s) 746, 859, 1132
BseLI CCNNNNNNNGG 2 cut(s) 26, 943
BseMII CTCAG 1 cut(s) 245
BseNI ACTGG 2 cut(s) 687, 1275
BseXI GCAGC 4 cut(s) 253, 421, 662, 756
Bsh1236I CGCG 2 cut(s) 41, 1199
BshFI GGCC 1 cut(s) 322
BshVI ATCGAT 1 cut(s) 367
BsiHKAI GWGCWC 1 cut(s) 1025
BsiHKCI CYCGRG 1 cut(s) 1448
BsiSI CCGG 2 cut(s) 851, 956
BsiWI CGTACG 1 cut(s) 42
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 2 cut(s) 26, 943
BsmAI GTCTC 2 cut(s) 249, 1085
BsmBI CGTCTC 1 cut(s) 1085
BsmFI GGGAC 1 cut(s) 152
BsnI GGCC 1 cut(s) 322
BsoBI CYCGRG 1 cut(s) 1448
Bsp119I TTCGAA 1 cut(s) 501
Bsp1286I GDGCHC 1 cut(s) 1025
Bsp13I TCCGGA 1 cut(s) 850
Bsp143I GATC 9 cut(s) 54, 204, 226, 481, 694, 828, 873, 981, 1401
BspACI CCGC 1 cut(s) 39
BspANI GGCC 1 cut(s) 322
BspCNI CTCAG 1 cut(s) 246
BspDI ATCGAT 1 cut(s) 367
BspEI TCCGGA 1 cut(s) 850
BspFNI CGCG 2 cut(s) 41, 1199
BspLI GGNNCC 2 cut(s) 168, 935
BspMI ACCTGC 1 cut(s) 1202
BspPI GGATC 2 cut(s) 221, 976
BspT104I TTCGAA 1 cut(s) 501
BsrI ACTGG 2 cut(s) 687, 1275
BssMI GATC 9 cut(s) 54, 204, 226, 481, 694, 828, 873, 981, 1401
BssNAI GTATAC 2 cut(s) 125, 546
Bst1107I GTATAC 2 cut(s) 125, 546
Bst2UI CCWGG 1 cut(s) 1442
Bst4CI ACNGT 4 cut(s) 658, 751, 1069, 1281
Bst6I CTCTTC 3 cut(s) 852, 1353, 1478
BstBI TTCGAA 1 cut(s) 501
BstC8I GCNNGC 4 cut(s) 490, 494, 578, 635
BstDEI CTNAG 3 cut(s) 231, 254, 401
BstENI CCTNNNNNAGG 1 cut(s) 24
BstF5I GGATG 3 cut(s) 746, 859, 1132
BstFNI CGCG 2 cut(s) 41, 1199
BstKTI GATC 9 cut(s) 57, 207, 229, 484, 697, 831, 876, 984, 1404
BstMAI GTCTC 2 cut(s) 249, 1085
BstMBI GATC 9 cut(s) 54, 204, 226, 481, 694, 828, 873, 981, 1401
BstNI CCWGG 1 cut(s) 1442
BstPAI GACNNNNGTC 2 cut(s) 164, 1067
BstSCI CCNGG 1 cut(s) 1440
BstUI CGCG 2 cut(s) 41, 1199
BstV1I GCAGC 4 cut(s) 253, 421, 662, 756
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
BstZ17I GTATAC 2 cut(s) 125, 546
Bsu15I ATCGAT 1 cut(s) 367
BsuI GTATCC 1 cut(s) 1267
BsuRI GGCC 1 cut(s) 322
BsuTUI ATCGAT 1 cut(s) 367
BtsCI GGATG 3 cut(s) 746, 859, 1132
BveI ACCTGC 1 cut(s) 1202
Cac8I GCNNGC 4 cut(s) 490, 494, 578, 635
CaiI CAGNNNCTG 1 cut(s) 266
Cfr13I GGNCC 1 cut(s) 166
ClaI ATCGAT 1 cut(s) 367
Csp6I GTAC 5 cut(s) 43, 180, 307, 861, 1268
CviAII CATG 2 cut(s) 904, 1421
CviQI GTAC 5 cut(s) 43, 180, 307, 861, 1268
DdeI CTNAG 3 cut(s) 231, 254, 401
DpnI GATC 9 cut(s) 56, 206, 228, 483, 696, 830, 875, 983, 1403
DpnII GATC 9 cut(s) 54, 204, 226, 481, 694, 828, 873, 981, 1401
DraI TTTAAA 1 cut(s) 454
DraIII CACNNNGTG 1 cut(s) 153
EaeI YGGCCR 1 cut(s) 320
Eam1104I CTCTTC 3 cut(s) 852, 1353, 1478
EarI CTCTTC 3 cut(s) 852, 1353, 1478
Eco32I GATATC 1 cut(s) 961
Eco47I GGWCC 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 537
Eco88I CYCGRG 1 cut(s) 1448
EcoNI CCTNNNNNAGG 1 cut(s) 24
EcoO109I RGGNCCY 1 cut(s) 166
EcoRI GAATTC 2 cut(s) 193, 1255
EcoRII CCWGG 1 cut(s) 1440
EcoRV GATATC 1 cut(s) 961
EcoT22I ATGCAT 1 cut(s) 1306
Esp3I CGTCTC 1 cut(s) 1085
FaeI CATG 2 cut(s) 907, 1424
FalI AAGNNNNNCTT 2 cut(s) 1200, 1232
FaqI GGGAC 1 cut(s) 152
FatI CATG 2 cut(s) 903, 1420
FauNDI CATATG 2 cut(s) 886, 1306
FbaI TGATCA 2 cut(s) 481, 1401
FblI GTMKAC 2 cut(s) 124, 545
Fnu4HI GCNGC 4 cut(s) 267, 410, 676, 745
FokI GGATG 3 cut(s) 753, 866, 1139
Fsp4HI GCNGC 4 cut(s) 267, 410, 676, 745
FspBI CTAG 2 cut(s) 335, 584
GluI GCNGC 4 cut(s) 267, 410, 676, 745
HaeIII GGCC 1 cut(s) 322
HapII CCGG 2 cut(s) 851, 956
Hin1II CATG 2 cut(s) 907, 1424
HincII GTYRAC 1 cut(s) 1195
HindII GTYRAC 1 cut(s) 1195
HinfI GANTC 4 cut(s) 250, 364, 842, 1232
HpaI GTTAAC 1 cut(s) 1195
HpaII CCGG 2 cut(s) 851, 956
HphI GGTGA 2 cut(s) 640, 1331
Hpy166II GTNNAC 5 cut(s) 125, 473, 546, 648, 1195
Hpy188I TCNGA 6 cut(s) 249, 255, 301, 517, 1261, 1471
Hpy188III TCNNGA 9 cut(s) 75, 335, 344, 385, 584, 716, 851, 1138, 1450
Hpy8I GTNNAC 5 cut(s) 125, 473, 546, 648, 1195
HpyAV CCTTC 2 cut(s) 268, 512
HpyCH4III ACNGT 4 cut(s) 658, 751, 1069, 1281
HpyCH4IV ACGT 2 cut(s) 178, 1094
HpyCH4V TGCA 8 cut(s) 432, 633, 678, 744, 952, 1293, 1304, 1436
HpyF3I CTNAG 3 cut(s) 231, 254, 401
HpySE526I ACGT 2 cut(s) 178, 1094
Hsp92II CATG 2 cut(s) 907, 1424
Kpn2I TCCGGA 1 cut(s) 850
Ksp22I TGATCA 2 cut(s) 481, 1401
KspAI GTTAAC 1 cut(s) 1195
Kzo9I GATC 9 cut(s) 54, 204, 226, 481, 694, 828, 873, 981, 1401
Lsp1109I GCAGC 4 cut(s) 253, 421, 662, 756
LweI GCATC 4 cut(s) 253, 419, 731, 1423
MaeI CTAG 2 cut(s) 335, 584
MaeII ACGT 2 cut(s) 178, 1094
MaeIII GTNAC 3 cut(s) 626, 751, 1069
MalI GATC 9 cut(s) 56, 206, 228, 483, 696, 830, 875, 983, 1403
MboI GATC 9 cut(s) 54, 204, 226, 481, 694, 828, 873, 981, 1401
MfeI CAATTG 1 cut(s) 1288
MflI RGATCY 1 cut(s) 226
MhlI GDGCHC 1 cut(s) 1025
MlsI TGGCCA 1 cut(s) 322
MluCI AATT 9 cut(s) 193, 239, 295, 466, 1113, 1248, 1255, 1288, 1340
MluI ACGCGT 1 cut(s) 1197
MluNI TGGCCA 1 cut(s) 322
MmeI TCCRAC 1 cut(s) 718
MnlI CCTC 8 cut(s) 279, 559, 793, 826, 900, 1236, 1477, 1479
Mox20I TGGCCA 1 cut(s) 322
Mph1103I ATGCAT 1 cut(s) 1306
MroI TCCGGA 1 cut(s) 850
MroXI GAANNNNTTC 3 cut(s) 34, 813, 1049
MscI TGGCCA 1 cut(s) 322
MseI TTAA 7 cut(s) 311, 453, 702, 867, 972, 1194, 1224
MslI CAYNNNNRTG 3 cut(s) 417, 539, 1352
Msp20I TGGCCA 1 cut(s) 322
MspA1I CMGCKG 1 cut(s) 940
MspI CCGG 2 cut(s) 851, 956
MspR9I CCNGG 1 cut(s) 1442
MunI CAATTG 1 cut(s) 1288
MvaI CCWGG 1 cut(s) 1442
MvnI CGCG 2 cut(s) 41, 1199
NdeI CATATG 2 cut(s) 886, 1306
NdeII GATC 9 cut(s) 54, 204, 226, 481, 694, 828, 873, 981, 1401
NlaIII CATG 2 cut(s) 907, 1424
NlaIV GGNNCC 2 cut(s) 168, 935
NmuCI GTSAC 2 cut(s) 626, 1069
NsiI ATGCAT 1 cut(s) 1306
NspV TTCGAA 1 cut(s) 501
PaeR7I CTCGAG 1 cut(s) 1448
PcsI WCGNNNNNNNCGW 1 cut(s) 869
PdmI GAANNNNTTC 3 cut(s) 34, 813, 1049
PfeI GAWTC 4 cut(s) 250, 364, 842, 1232
Pfl23II CGTACG 1 cut(s) 42
PflMI CCANNNNNTGG 1 cut(s) 943
PfoI TCCNGGA 1 cut(s) 1440
PkrI GCNGC 4 cut(s) 268, 411, 677, 746
PpuMI RGGWCCY 1 cut(s) 166
PshAI GACNNNNGTC 2 cut(s) 164, 1067
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 1440
PspGI CCWGG 1 cut(s) 1440
PspLI CGTACG 1 cut(s) 42
PspN4I GGNNCC 2 cut(s) 168, 935
PspPI GGNCC 1 cut(s) 166
PspPPI RGGWCCY 1 cut(s) 166
PsrI GAACNNNNNNTAC 2 cut(s) 917, 949
PstNI CAGNNNCTG 1 cut(s) 266
PsuI RGATCY 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 940
RsaI GTAC 5 cut(s) 44, 181, 308, 862, 1269
RsaNI GTAC 5 cut(s) 43, 180, 307, 861, 1268
RseI CAYNNNNRTG 3 cut(s) 417, 539, 1352
SaqAI TTAA 7 cut(s) 311, 453, 702, 867, 972, 1194, 1224
SatI GCNGC 4 cut(s) 267, 410, 676, 745
Sau3AI GATC 9 cut(s) 54, 204, 226, 481, 694, 828, 873, 981, 1401
Sau96I GGNCC 1 cut(s) 166
ScaI AGTACT 1 cut(s) 308
ScrFI CCNGG 1 cut(s) 1442
SduI GDGCHC 1 cut(s) 1025
SfaNI GCATC 4 cut(s) 253, 419, 731, 1423
Sfr274I CTCGAG 1 cut(s) 1448
SfuI TTCGAA 1 cut(s) 501
SinI GGWCC 1 cut(s) 166
SlaI CTCGAG 1 cut(s) 1448
SmiMI CAYNNNNRTG 3 cut(s) 417, 539, 1352
SmlI CTYRAG 4 cut(s) 383, 605, 716, 1448
SmoI CTYRAG 4 cut(s) 383, 605, 716, 1448
Sse9I AATT 9 cut(s) 193, 239, 295, 466, 1113, 1248, 1255, 1288, 1340
SsiI CCGC 1 cut(s) 39
SspMI CTAG 2 cut(s) 335, 584
StyD4I CCNGG 1 cut(s) 1440
TaaI ACNGT 4 cut(s) 658, 751, 1069, 1281
TaiI ACGT 2 cut(s) 181, 1097
TaqI TCGA 8 cut(s) 76, 158, 367, 501, 831, 872, 1431, 1449
TasI AATT 9 cut(s) 193, 239, 295, 466, 1113, 1248, 1255, 1288, 1340
TatI WGTACW 1 cut(s) 306
TfiI GAWTC 4 cut(s) 250, 364, 842, 1232
Tru1I TTAA 7 cut(s) 311, 453, 702, 867, 972, 1194, 1224
Tru9I TTAA 7 cut(s) 311, 453, 702, 867, 972, 1194, 1224
TseFI GTSAC 2 cut(s) 626, 1069
TseI GCWGC 4 cut(s) 266, 409, 675, 744
Tsp45I GTSAC 2 cut(s) 626, 1069
TspDTI ATGAA 5 cut(s) 206, 435, 629, 1160, 1349
TspGWI ACGGA 1 cut(s) 852
Van91I CCANNNNNTGG 1 cut(s) 943
VpaK11BI GGWCC 1 cut(s) 166
XagI CCTNNNNNAGG 1 cut(s) 24
XapI RAATTY 2 cut(s) 193, 1255
XbaI TCTAGA 2 cut(s) 334, 583
XhoI CTCGAG 1 cut(s) 1448
XmiI GTMKAC 2 cut(s) 124, 545
XmnI GAANNNNTTC 3 cut(s) 34, 813, 1049
XspI CTAG 2 cut(s) 335, 584
ZrmI AGTACT 1 cut(s) 308
Zsp2I ATGCAT 1 cut(s) 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.