Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0004347

Physical Location & Seq

Genomic Coordinates Reverse (-)

64449968 .. 64455927

Gene Structure 5960 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000030614

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2259 bp

ATGTCCCTTCCTCTGACCTACTTCGACTCGCTCTTTCTAAGGTTTCCACCCGTACAACGCCTTTACTTCTATGAATTAACATCTTCTTCCTCTTCTTCTTCCACTAGTACTGATTTTGCAGTGCTTGCCTATCTCAAAACCTTGCTCTCTCTCACTCTCAAACACTTTGCACCTCTCGCCGGAAACCTAACTTGGCCTCAAGACTCCCACACACCCATTCTCAGCTATGTCCAAGGCGACGCCGTTTCACTCACAGTAGCTGAGTCCGATGCTGATAATTTCGACCACCTTTCGAGCAACAAGTCTTTCTCAAATCCAAAGCTTACCATCCACTTGTTCCCCAATTGGAGTCGTCTCATGAACGAACTGCAGCCATTGCATTCAAATAACCCTATTTCCTGGCCGTGGCTTCGCCATTGGAACAGCCATGCACCATGCGATCCTCGATGGCAAAACATCAATCTCCTCAAGCCATTATATGATAGAACGGTCATCTATGATCTGACTGGGCTCGGACTCGAATCCATCTACTTAAACGATTGGCAAAACTTGGATGGCCCCAACAATAGAAGCGTAAAGTCACAGCAATTAGCGAAACTATATATAAAGGGTTTAGACAAGAATGGGGTTTTGAAAGGAGCCCACGAGCTTTACTTTTCGAAAACCAAGGACTTGTTTCAGGTTAAGGGGATATATTCTACTGCTGGTTCGCACCGATTTCAGATTTACAATACGAACTTTGGCTGGGGTAGGCCGAAAAAAGTTGAGGTGGTCTCTATAGATAGGACCGGAGCGATTTCAGTCTCCGATTCCAAGAATGGTGGTGGAGGTATAGAGATTGGGGTGGTTTTGAAAACAGATTGTATGGAAGCATTTGCTTCTCTATTTGCTGTCAGGGCTCCTCAACTCCACCTCCTGCGCTCCATTGTTTCGACCCCAACTCTGTACCCTGACCGCACCCACAAGCCAAACCCACCAATCCAGTGTCTCCGTTCGTCAAAACGGAGCTCCGGCCCGAAGGAACACTCTCGGATTCTGAGAACCGAGGCCGCTGTTAAAAACATTGAGAGGAAAGCAAATTCCAACAAGTACAATAACCTTTGGCCTAAGGCTGTCTTGGAGGCCTTGGATGAGGCGGTTGCCAACAATCTCTGGGAGACTGCTCTTAAGATATTTGGACTACTGCACAAGCAACAATGGTATGAGCCAAGATGCCAAACTTACGCTAAATTGTTCATGATGCTTGGAAAGTGCAAGCAACCTGAGCAGGCTGGCTTACTTTTCGAGCTCATGTTAACTGATGGGCTCAAACCAACTGTTGACGTCTACACAGCTCTTGTTAGTGTTTATGGCAAAAGTGGCCTCTTTGATAAGGCGTTCTCTACCGTTGATGATATGAAGTCAATATCCGATTGCAAACCAGATGTGTATACATATTCAATTCTTATAAATTGCTGCACTAAATTCCGTCATTATGATCTGATTGAGCAAGTTCTGGCTGAGATGTCATATCTGGGAATTGGATGTAACACAGTCATATACAATACTCTTATCGATGGTTATGGTAAAGCTGAAATGTTTGAACTGATGGAGGACTCATTGACAGATATGATTGAAAGTGGCAGCTGCCTTCCTGACGTTTTCACATTCAATTCTTTCCTTGGCGCTTATGGGAAAAGTGGGCAGATTGACAAGATGGAGAAGTGGTATGATGAATTTCAGCTAATGGGAATAAAGCCAGACCTTAAGACATTTAATGTCCTGATTAAATCATATGGGAAAGCAAGAATGTATGAAAAGATGGGGTCTGTTATGGAGTTTATGGAGAAAAGGCATTTCACCCCAACTGTTGTTACTTATAACATAGTAATTGAGGTATTTGGGAAAGCTGGAAATATTGTGAAGATGGAGGAATACTTCAGAAAAATGAAGCACCAAGGAATGAAGCCCAGCTCTATCGTTAGTGCGTACAGCAAAGCTGGGAATATAAATAAAGTTGATTCCATTTTGAGGCAAGTAGAGAATTCAGATGTTATACTTGATGCAGCTTTTTTCAACTGTATTATTAGTGCTTATGGTCGGGCTGGTGATATAAATAAGATGGGTGAGATATTTTTGACCATGGAAGAGAAAAAATGTGTTCCTGATCATATCACCTTTGCTACCATGATCCAAGCCTGCAATGCACTAGGCATGACTGCGGCTGTCGAAGATATAAAAAACAGGATGATTACCACCAATGACAGTTCAGGTATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

753

Amino Acids

84.8

Weight (kDa)

8.33

Isoelectric Point (pI)

39.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Transferase	PF02458	230 - 294	7e-07	Transferase family
PPR_2	PF13041	438 - 487	2.2e-12	PPR repeat family
PPR_3	PF13812	464 - 523	2.4e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	545 - 593	3.8e-11	PPR repeat family
PPR_3	PF13812	571 - 629	1.1e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	584 - 627	2.1e-08	PPR repeat family
PPR_2	PF13041	615 - 652	8e-07	PPR repeat family
PPR	PF01535	618 - 647	1e-05	PPR repeat
PPR_3	PF13812	668 - 727	8.6e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	681 - 727	1.7e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0004347)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53170 AT3G53170 AT3G53170 AT3G53170
fragaria_vesca	FvH4_7g01420
malus_domestica	MD02G1305700.v1.1 MD02G1306000.v1.1
prunus_persica	Prupe.2G019900_v2.0.a1 Prupe.2G019900_v2.0.a1 Prupe.2G019900_v2.0.a1 Prupe.2G019900_v2.0.a1 Prupe.2G019900_v2.0.a1 Prupe.2G019900_v2.0.a1
pyrus_communis	pycom02g25620
rosa_chinensis	RchiOBHm_Chr1g0317271
rosa_laevigata	RLG00000029578 RLG00000030614 RLG00000030617
rosa_multiflora	Rmu_sc0005698.1_g000002 Rmu_sc0007593.1_g000003
rosa_roxburghii	Rroxscaffold_4G00327510 Rroxscaffold_4G00330560
rosa_rugosa	Rorug01G0015300 Rorug01G0015400 Rorug01G0015400
rosa_samantha	Rh1AG026400 Rh1BG022700 Rh1CG024600 Rh1CG049800 Rh1DG047300 Rh1DG054300
rosa_wichuraiana	Rw1G001840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1448, 1860
AatII	GACGTC	1 cut(s)	1326
AccI	GTMKAC	2 cut(s)	1326, 1430
AciI	CCGC	4 cut(s)	955, 1050, 1136, 2201
AclWI	GGATC	2 cut(s)	434, 2164
AcoI	YGGCCR	1 cut(s)	401
AcsI	RAATTY	4 cut(s)	1078, 1463, 1715, 2023
AcuI	CTGAAG	1 cut(s)	1903
AcyI	GRCGYC	2 cut(s)	240, 1323
AfaI	GTAC	5 cut(s)	54, 109, 947, 1091, 1970
AfiI	CCNNNNNNNGG	3 cut(s)	179, 405, 2010
AflII	CTTAAG	2 cut(s)	1166, 1745
AgsI	TTSAA	8 cut(s)	384, 634, 853, 1440, 1583, 1616, 1651, 2056
AhlI	ACTAGT	1 cut(s)	104
AjnI	CCWGG	1 cut(s)	398
AjuI	GAANNNNNNNTTGG	2 cut(s)	175, 207
Alw21I	GWGCWC	2 cut(s)	1010, 1290
Alw26I	GTCTC	5 cut(s)	359, 778, 808, 992, 1151
AlwI	GGATC	2 cut(s)	434, 2164
AlwNI	CAGNNNCTG	1 cut(s)	260
AoxI	GGCC	9 cut(s)	194, 401, 556, 752, 1012, 1047, 1103, 1122, 1360
ApeKI	GCWGC	5 cut(s)	370, 1455, 1623, 1626, 2045
ApoI	RAATTY	4 cut(s)	1078, 1463, 1715, 2023
AspLEI	GCGC	2 cut(s)	921, 1667
AspS9I	GGNCC	3 cut(s)	557, 786, 1013
AsuHPI	GGTGA	4 cut(s)	1831, 2099, 2117, 2146
AsuII	TTCGAA	1 cut(s)	659
AvaII	GGWCC	1 cut(s)	786
AxyI	CCTNAGG	1 cut(s)	1107
BanII	GRGCYC	6 cut(s)	513, 643, 901, 1010, 1290, 1308
BauI	CACGAG	1 cut(s)	644
Bbv12I	GWGCWC	2 cut(s)	1010, 1290
BbvI	GCAGC	5 cut(s)	382, 1442, 1613, 1635, 2057
BceAI	ACGGC	2 cut(s)	227, 388
BciT130I	CCWGG	1 cut(s)	400
BclI	TGATCA	1 cut(s)	2146
BcoDI	GTCTC	5 cut(s)	359, 778, 808, 992, 1151
BcuI	ACTAGT	1 cut(s)	104
BfaI	CTAG	2 cut(s)	105, 2189
BfmI	CTRYAG	2 cut(s)	368, 777
BfoI	RGCGCY	1 cut(s)	1668
BfrI	CTTAAG	2 cut(s)	1166, 1745
BisI	GCNGC	7 cut(s)	371, 1050, 1456, 1624, 1627, 2046, 2202
BlsI	GCNGC	7 cut(s)	372, 1051, 1457, 1625, 1628, 2047, 2203
BmcAI	AGTACT	1 cut(s)	109
Bme1390I	CCNGG	1 cut(s)	400
Bme18I	GGWCC	1 cut(s)	786
BmgT120I	GGNCC	3 cut(s)	557, 786, 1013
BmiI	GGNNCC	3 cut(s)	559, 640, 900
BmrFI	CCNGG	1 cut(s)	400
BmrI	ACTGGG	1 cut(s)	516
BmsI	GCATC	4 cut(s)	259, 1202, 1230, 2032
BmuI	ACTGGG	1 cut(s)	516
BplI	GAGNNNNNCTC	2 cut(s)	758, 790
Bpu10I	CCTNAGC	1 cut(s)	1263
Bpu14I	TTCGAA	1 cut(s)	659
BpuEI	CTTGAG	2 cut(s)	183, 452
Bsa29I	ATCGAT	1 cut(s)	1554
BsaHI	GRCGYC	2 cut(s)	240, 1323
BsaI	GGTCTC	1 cut(s)	778
BsaJI	CCNNGG	8 cut(s)	232, 404, 666, 1044, 1125, 1660, 1936, 2121
BsaWI	WCCGGW	1 cut(s)	788
BsaXI	ACNNNNNCTCC	4 cut(s)	897, 927, 1691, 1721
Bsc4I	CCNNNNNNNGG	3 cut(s)	179, 405, 2010
Bse1I	ACTGG	2 cut(s)	511, 982
Bse21I	CCTNAGG	1 cut(s)	1107
Bse3DI	GCAATG	2 cut(s)	374, 2188
BseBI	CCWGG	1 cut(s)	400
BseCI	ATCGAT	1 cut(s)	1554
BseDI	CCNNGG	8 cut(s)	232, 404, 666, 1044, 1125, 1660, 1936, 2121
BseGI	GGATG	5 cut(s)	327, 559, 1135, 1529, 2232
BseLI	CCNNNNNNNGG	3 cut(s)	179, 405, 2010
BseMI	GCAATG	2 cut(s)	374, 2188
BseMII	CTCAG	5 cut(s)	235, 252, 1028, 1254, 1491
BseNI	ACTGG	2 cut(s)	511, 982
BseRI	GAGGAG	2 cut(s)	455, 891
BseXI	GCAGC	5 cut(s)	382, 1442, 1613, 1635, 2057
BseYI	CCCAGC	3 cut(s)	744, 1949, 1979
BsgI	GTGCAG	2 cut(s)	1169, 1441
BshFI	GGCC	9 cut(s)	196, 403, 558, 754, 1014, 1049, 1105, 1124, 1362
BshVI	ATCGAT	1 cut(s)	1554
BsiHKAI	GWGCWC	2 cut(s)	1010, 1290
BsiSI	CCGG	3 cut(s)	180, 789, 1011
BslI	CCNNNNNNNGG	3 cut(s)	179, 405, 2010
BsmAI	GTCTC	5 cut(s)	359, 778, 808, 992, 1151
BsmBI	CGTCTC	1 cut(s)	359
BsmI	GAATGC	1 cut(s)	379
BsnI	GGCC	9 cut(s)	196, 403, 558, 754, 1014, 1049, 1105, 1124, 1362
Bso31I	GGTCTC	1 cut(s)	778
Bsp119I	TTCGAA	1 cut(s)	659
Bsp1286I	GDGCHC	6 cut(s)	513, 643, 901, 1010, 1290, 1308
Bsp143I	GATC	5 cut(s)	439, 499, 1477, 2146, 2169
Bsp19I	CCATGG	1 cut(s)	2121
BspACI	CCGC	4 cut(s)	955, 1050, 1136, 2201
BspANI	GGCC	9 cut(s)	196, 403, 558, 754, 1014, 1049, 1105, 1124, 1362
BspCNI	CTCAG	5 cut(s)	234, 253, 1029, 1255, 1492
BspDI	ATCGAT	1 cut(s)	1554
BspHI	TCATGA	2 cut(s)	357, 1236
BspLI	GGNNCC	3 cut(s)	559, 640, 900
BspMAI	CTGCAG	1 cut(s)	372
BspPI	GGATC	2 cut(s)	434, 2164
BspT104I	TTCGAA	1 cut(s)	659
BspTI	CTTAAG	2 cut(s)	1166, 1745
BspTNI	GGTCTC	1 cut(s)	778
BsrDI	GCAATG	2 cut(s)	374, 2188
BsrI	ACTGG	2 cut(s)	511, 982
BssECI	CCNNGG	8 cut(s)	232, 404, 666, 1044, 1125, 1660, 1936, 2121
BssMI	GATC	5 cut(s)	439, 499, 1477, 2146, 2169
BssNAI	GTATAC	1 cut(s)	1431
BssNI	GRCGYC	2 cut(s)	240, 1323
BssSI	CACGAG	1 cut(s)	644
BssT1I	CCWWGG	6 cut(s)	232, 666, 1125, 1660, 1936, 2121
Bst1107I	GTATAC	1 cut(s)	1431
Bst2BI	CACGAG	1 cut(s)	644
Bst2UI	CCWGG	1 cut(s)	400
Bst4CI	ACNGT	8 cut(s)	256, 490, 1318, 1387, 1534, 1849, 2060, 2246
Bst6I	CTCTTC	2 cut(s)	97, 2121
BstACI	GRCGYC	2 cut(s)	240, 1323
BstAFI	CTTAAG	2 cut(s)	1166, 1745
BstAPI	GCANNNNNTGC	2 cut(s)	125, 376
BstBI	TTCGAA	1 cut(s)	659
BstC8I	GCNNGC	5 cut(s)	126, 1256, 1269, 1273, 2179
BstDEI	CTNAG	7 cut(s)	38, 221, 261, 1037, 1107, 1263, 1500
BstDSI	CCRYGG	2 cut(s)	404, 2121
BstF5I	GGATG	5 cut(s)	327, 559, 1135, 1529, 2232
BstH2I	RGCGCY	1 cut(s)	1668
BstHHI	GCGC	2 cut(s)	921, 1667
BstKTI	GATC	5 cut(s)	442, 502, 1480, 2149, 2172
BstMAI	GTCTC	5 cut(s)	359, 778, 808, 992, 1151
BstMBI	GATC	5 cut(s)	439, 499, 1477, 2146, 2169
BstMWI	GCNNNNNNNGC	7 cut(s)	125, 176, 376, 896, 1264, 1359, 2183
BstNI	CCWGG	1 cut(s)	400
BstSCI	CCNGG	1 cut(s)	398
BstSFI	CTRYAG	2 cut(s)	368, 777
BstV1I	GCAGC	5 cut(s)	382, 1442, 1613, 1635, 2057
BstZ17I	GTATAC	1 cut(s)	1431
Bsu15I	ATCGAT	1 cut(s)	1554
Bsu36I	CCTNAGG	1 cut(s)	1107
BsuRI	GGCC	9 cut(s)	196, 403, 558, 754, 1014, 1049, 1105, 1124, 1362
BsuTUI	ATCGAT	1 cut(s)	1554
BtgI	CCRYGG	2 cut(s)	404, 2121
BtsCI	GGATG	5 cut(s)	327, 559, 1135, 1529, 2232
BtsI	GCAGTG	1 cut(s)	126
BtsIMutI	CAGTG	2 cut(s)	126, 989
Cac8I	GCNNGC	5 cut(s)	126, 1256, 1269, 1273, 2179
CaiI	CAGNNNCTG	1 cut(s)	260
CciI	TCATGA	2 cut(s)	357, 1236
CfoI	GCGC	2 cut(s)	921, 1667
Cfr13I	GGNCC	3 cut(s)	557, 786, 1013
ClaI	ATCGAT	1 cut(s)	1554
CseI	GACGC	1 cut(s)	248
Csp6I	GTAC	5 cut(s)	53, 108, 946, 1090, 1969
CspCI	CAANNNNNGTGG	2 cut(s)	802, 837
CviAII	CATG	8 cut(s)	358, 428, 435, 1237, 1291, 2122, 2167, 2194
CviQI	GTAC	5 cut(s)	53, 108, 946, 1090, 1969
DdeI	CTNAG	7 cut(s)	38, 221, 261, 1037, 1107, 1263, 1500
DpnI	GATC	5 cut(s)	441, 501, 1479, 2148, 2171
DpnII	GATC	5 cut(s)	439, 499, 1477, 2146, 2169
EaeI	YGGCCR	1 cut(s)	401
Eam1104I	CTCTTC	2 cut(s)	97, 2121
EarI	CTCTTC	2 cut(s)	97, 2121
Ecl136II	GAGCTC	2 cut(s)	1008, 1288
Eco130I	CCWWGG	6 cut(s)	232, 666, 1125, 1660, 1936, 2121
Eco147I	AGGCCT	1 cut(s)	1124
Eco24I	GRGCYC	6 cut(s)	513, 643, 901, 1010, 1290, 1308
Eco31I	GGTCTC	1 cut(s)	778
Eco47I	GGWCC	1 cut(s)	786
Eco53kI	GAGCTC	2 cut(s)	1008, 1288
Eco57I	CTGAAG	1 cut(s)	1903
Eco81I	CCTNAGG	1 cut(s)	1107
EcoICRI	GAGCTC	2 cut(s)	1008, 1288
EcoRI	GAATTC	1 cut(s)	2023
EcoRII	CCWGG	1 cut(s)	398
EcoT14I	CCWWGG	6 cut(s)	232, 666, 1125, 1660, 1936, 2121
EcoT38I	GRGCYC	6 cut(s)	513, 643, 901, 1010, 1290, 1308
ErhI	CCWWGG	6 cut(s)	232, 666, 1125, 1660, 1936, 2121
Esp3I	CGTCTC	1 cut(s)	359
FaeI	CATG	8 cut(s)	361, 431, 438, 1240, 1294, 2125, 2170, 2197
FalI	AAGNNNNNCTT	2 cut(s)	1100, 1132
FatI	CATG	8 cut(s)	357, 427, 434, 1236, 1290, 2121, 2166, 2193
FauNDI	CATATG	1 cut(s)	1774
FbaI	TGATCA	1 cut(s)	2146
FblI	GTMKAC	2 cut(s)	1326, 1430
Fnu4HI	GCNGC	7 cut(s)	371, 1050, 1456, 1624, 1627, 2046, 2202
FokI	GGATG	5 cut(s)	314, 566, 1142, 1536, 2239
FriOI	GRGCYC	6 cut(s)	513, 643, 901, 1010, 1290, 1308
Fsp4HI	GCNGC	7 cut(s)	371, 1050, 1456, 1624, 1627, 2046, 2202
FspBI	CTAG	2 cut(s)	105, 2189
GlaI	GCGC	2 cut(s)	920, 1666
GluI	GCNGC	7 cut(s)	371, 1050, 1456, 1624, 1627, 2046, 2202
GsaI	CCCAGC	3 cut(s)	748, 1953, 1983
HaeII	RGCGCY	1 cut(s)	1668
HaeIII	GGCC	9 cut(s)	196, 403, 558, 754, 1014, 1049, 1105, 1124, 1362
HapII	CCGG	3 cut(s)	180, 789, 1011
HgaI	GACGC	1 cut(s)	248
HhaI	GCGC	2 cut(s)	921, 1667
Hin1I	GRCGYC	2 cut(s)	240, 1323
Hin1II	CATG	8 cut(s)	361, 431, 438, 1240, 1294, 2125, 2170, 2197
Hin6I	GCGC	2 cut(s)	919, 1665
HinP1I	GCGC	2 cut(s)	919, 1665
HincII	GTYRAC	2 cut(s)	1296, 1321
HindII	GTYRAC	2 cut(s)	1296, 1321
HindIII	AAGCTT	1 cut(s)	320
HpaI	GTTAAC	1 cut(s)	1296
HpaII	CCGG	3 cut(s)	180, 789, 1011
HphI	GGTGA	4 cut(s)	1831, 2099, 2117, 2146
Hpy166II	GTNNAC	4 cut(s)	1296, 1321, 1327, 1431
Hpy188III	TCNNGA	6 cut(s)	200, 358, 1237, 1634, 1762, 2144
Hpy8I	GTNNAC	4 cut(s)	1296, 1321, 1327, 1431
Hpy99I	CGWCG	1 cut(s)	242
HpyAV	CCTTC	3 cut(s)	17, 1012, 1640
HpyCH4III	ACNGT	8 cut(s)	256, 490, 1318, 1387, 1534, 1849, 2060, 2246
HpyCH4IV	ACGT	2 cut(s)	1323, 1638
HpyF10VI	GCNNNNNNNGC	7 cut(s)	125, 176, 376, 896, 1264, 1359, 2183
HpyF3I	CTNAG	7 cut(s)	38, 221, 261, 1037, 1107, 1263, 1500
HpySE526I	ACGT	2 cut(s)	1323, 1638
Hsp92I	GRCGYC	2 cut(s)	240, 1323
Hsp92II	CATG	8 cut(s)	361, 431, 438, 1240, 1294, 2125, 2170, 2197
HspAI	GCGC	2 cut(s)	919, 1665
Ksp22I	TGATCA	1 cut(s)	2146
KspAI	GTTAAC	1 cut(s)	1296
Kzo9I	GATC	5 cut(s)	439, 499, 1477, 2146, 2169
LmnI	GCTCC	6 cut(s)	638, 791, 904, 926, 1005, 1013
Lsp1109I	GCAGC	5 cut(s)	382, 1442, 1613, 1635, 2057
LweI	GCATC	4 cut(s)	259, 1202, 1230, 2032
MaeI	CTAG	2 cut(s)	105, 2189
MaeII	ACGT	2 cut(s)	1323, 1638
MaeIII	GTNAC	3 cut(s)	579, 1526, 1852
MalI	GATC	5 cut(s)	441, 501, 1479, 2148, 2171
MboI	GATC	5 cut(s)	439, 499, 1477, 2146, 2169
MboII	GAAGA	8 cut(s)	75, 78, 84, 87, 90, 1915, 2138, 2222
MfeI	CAATTG	1 cut(s)	343
MhlI	GDGCHC	6 cut(s)	513, 643, 901, 1010, 1290, 1308
MlyI	GAGTC	6 cut(s)	20, 197, 272, 358, 510, 1589
MmeI	TCCRAC	1 cut(s)	1107
MseI	TTAA	9 cut(s)	77, 533, 684, 1056, 1167, 1295, 1746, 1755, 1767
MspA1I	CMGCKG	2 cut(s)	1052, 1626
MspCI	CTTAAG	2 cut(s)	1166, 1745
MspI	CCGG	3 cut(s)	180, 789, 1011
MspR9I	CCNGG	1 cut(s)	400
MunI	CAATTG	1 cut(s)	343
Mva1269I	GAATGC	1 cut(s)	379
MvaI	CCWGG	1 cut(s)	400
MwoI	GCNNNNNNNGC	7 cut(s)	125, 176, 376, 896, 1264, 1359, 2183
NcoI	CCATGG	1 cut(s)	2121
NdeI	CATATG	1 cut(s)	1774
NdeII	GATC	5 cut(s)	439, 499, 1477, 2146, 2169
NlaIII	CATG	8 cut(s)	361, 431, 438, 1240, 1294, 2125, 2170, 2197
NlaIV	GGNNCC	3 cut(s)	559, 640, 900
NmuCI	GTSAC	1 cut(s)	579
NspV	TTCGAA	1 cut(s)	659
PagI	TCATGA	2 cut(s)	357, 1236
PceI	AGGCCT	1 cut(s)	1124
PctI	GAATGC	1 cut(s)	379
PfeI	GAWTC	4 cut(s)	521, 809, 1033, 2000
PkrI	GCNGC	7 cut(s)	372, 1051, 1457, 1625, 1628, 2047, 2203
PleI	GAGTC	6 cut(s)	20, 197, 271, 357, 510, 1589
PpsI	GAGTC	6 cut(s)	20, 197, 271, 357, 510, 1589
PsiI	TTATAA	2 cut(s)	1448, 1860
Psp124BI	GAGCTC	2 cut(s)	1010, 1290
Psp6I	CCWGG	1 cut(s)	398
PspFI	CCCAGC	3 cut(s)	744, 1949, 1979
PspGI	CCWGG	1 cut(s)	398
PspN4I	GGNNCC	3 cut(s)	559, 640, 900
PspPI	GGNCC	3 cut(s)	557, 786, 1013
PstI	CTGCAG	1 cut(s)	372
PstNI	CAGNNNCTG	1 cut(s)	260
PvuII	CAGCTG	1 cut(s)	1626
RsaI	GTAC	5 cut(s)	54, 109, 947, 1091, 1970
RsaNI	GTAC	5 cut(s)	53, 108, 946, 1090, 1969
SacI	GAGCTC	2 cut(s)	1010, 1290
SaqAI	TTAA	9 cut(s)	77, 533, 684, 1056, 1167, 1295, 1746, 1755, 1767
SatI	GCNGC	7 cut(s)	371, 1050, 1456, 1624, 1627, 2046, 2202
Sau3AI	GATC	5 cut(s)	439, 499, 1477, 2146, 2169
Sau96I	GGNCC	3 cut(s)	557, 786, 1013
ScaI	AGTACT	1 cut(s)	109
SchI	GAGTC	6 cut(s)	20, 197, 272, 358, 510, 1589
ScrFI	CCNGG	1 cut(s)	400
SduI	GDGCHC	6 cut(s)	513, 643, 901, 1010, 1290, 1308
SfaNI	GCATC	4 cut(s)	259, 1202, 1230, 2032
SfcI	CTRYAG	2 cut(s)	368, 777
SfuI	TTCGAA	1 cut(s)	659
SinI	GGWCC	1 cut(s)	786
SmlI	CTYRAG	4 cut(s)	198, 467, 1166, 1745
SmoI	CTYRAG	4 cut(s)	198, 467, 1166, 1745
SpeI	ACTAGT	1 cut(s)	104
SseBI	AGGCCT	1 cut(s)	1124
SsiI	CCGC	4 cut(s)	955, 1050, 1136, 2201
SspI	AATATT	1 cut(s)	1897
SspMI	CTAG	2 cut(s)	105, 2189
SstI	GAGCTC	2 cut(s)	1010, 1290
StuI	AGGCCT	1 cut(s)	1124
StyD4I	CCNGG	1 cut(s)	398
StyI	CCWWGG	6 cut(s)	232, 666, 1125, 1660, 1936, 2121
TaaI	ACNGT	8 cut(s)	256, 490, 1318, 1387, 1534, 1849, 2060, 2246
TaiI	ACGT	2 cut(s)	1326, 1641
TatI	WGTACW	2 cut(s)	107, 1089
TauI	GCSGC	2 cut(s)	1052, 2204
TfiI	GAWTC	4 cut(s)	521, 809, 1033, 2000
Tru1I	TTAA	9 cut(s)	77, 533, 684, 1056, 1167, 1295, 1746, 1755, 1767
Tru9I	TTAA	9 cut(s)	77, 533, 684, 1056, 1167, 1295, 1746, 1755, 1767
TscAI	CASTG	2 cut(s)	126, 989
TseFI	GTSAC	1 cut(s)	579
TseI	GCWGC	5 cut(s)	370, 1455, 1623, 1626, 2045
Tsp45I	GTSAC	1 cut(s)	579
TspDTI	ATGAA	8 cut(s)	87, 374, 1225, 1412, 1728, 1809, 1943, 1958
TspGWI	ACGGA	3 cut(s)	980, 1018, 1457
TspRI	CASTG	2 cut(s)	126, 989
Vha464I	CTTAAG	2 cut(s)	1166, 1745
VpaK11BI	GGWCC	1 cut(s)	786
XapI	RAATTY	4 cut(s)	1078, 1463, 1715, 2023
XmiI	GTMKAC	2 cut(s)	1326, 1430
XspI	CTAG	2 cut(s)	105, 2189
ZraI	GACGTC	1 cut(s)	1324
ZrmI	AGTACT	1 cut(s)	109

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.