MD02G1305700.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004347
Physical Location & Seq
Forward (+)
35880483 .. 35889445
8963 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1305700.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGGAGCGTCAACTCCTTTATCCCACTAACCTGCGCGTCTCGACCTCAACCTCCTTAACCGGCGCCACATATTGGACGACGTTCTACTCTGAACACACCCACAAACCAAACCCAACAATCCGCTGCCTCCGTTTATCGAAACGGAGCTCCGACCCGTCTCTGACGAAGGAGCAGAAGAATCTGTCTCAACTCCTCCGAACGGACGCCGCCGTGAGAAACATTGAGAGGAAAGCCAACTCCAAGAAGTACAACAACCTTTGGCCCAAGGCCGTTTTGGAGGCTCTGGACGAGGCTATTGCAGCCAATCTCTGGGAAACTGCTCTCAAGATTTTTGGTCTACTTCGTAAGCAGCACTGGTATGAGCCGAGATGCCAAACGTACACCAAGCTGCTGATGATGCTTGGCAAGTGCAGGCAACCTGAGCAGGCCAGCTTGCTTTTTGAGCTCATGTTGAGTGATGGGCTCAAACCTACTGTTGATGTCTACACAGCTCTTGTTAGTGTTTATGGCAAAAGTGGCCTCCTTGACAAGGCATTCTCTACCGTTGATGATATGAAGTCAGTTTCTGACTGCAAACCAGACGTATATACATATTCTATTCTTATTAATTCCTGCACCAAGTTCAATCGTCCTGACCTGATTGAACGCGTTCTTGCTGAGATGTCGTACCTGGGGATTGGATGCAACACAGTCATATACAATACCCTAATTGATGGATATGGTAAGGCTGAAATGTTTGAACTGATGGAGGAAACGTTGACAAATATGATTGAAAGTGGCAGCTGCCTTCCTGATGTTTTCACTTTCAATTCTTTTCTCAGTGCTTATGGGAATTGTGGGCAGATAGAGAGGATGGAGAAGTGGTATGATGAATTTCAGCTGATGGGAATAAGGCCAGACCCTAAGACATTCAATATCCTGATTAAATCATACGGGAAGGCAAGAATGTACGAAAAGATGAGATCTGTTATGGAATTTATGAAGAAAAGGTTTTTCACCCCAACTATTGTTACTTATAATATTGTAATTGAGGTATTCGGTAAAGCTGGAAATATTGAGAAGATGGACGAATACTTCCGAAGAATGAAGCATCAAGGAATGAAGCCCAACTCTATCACCTATTGTTCTCTTGTTAGTGCGTATAGCAAAGCTGGGAGTATAAGTAAAGTTGATTCCATTTTGAGGCAAGTAGAGAATTCGGATGTCATGTTAGATACACCTTTTTTCAACTGTATTATTAGTGCTTATTGTCGCACTGGTGACCTAAGAAAGGTAAGCGAACTGTTTTTAGCAATGAAAGAGAAAAAATGTGTGCCTGATCATATCACATTTGCTACCATGATCCAAGCCTACAATGAACGAGGCATGAATGAGGCTGCTCAAGACTTGAAAAATAGGATGATTACCACCAAGGAAAATTCAGGCACAAGGTTGATTGGATGCTAG

Protein Analysis

482

Amino Acids

55.11

Weight (kDa)

8.89

Isoelectric Point (pI)

34.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 146 - 206 2.5e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 153 - 186 5.4e-06 PPR repeat
PPR_2 PF13041 157 - 207 2.2e-12 PPR repeat family
PPR_3 PF13812 216 - 278 5.6e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 229 - 269 5.1e-06 PPR repeat family
PPR_2 PF13041 264 - 312 9.1e-11 PPR repeat family
PPR_3 PF13812 290 - 348 3e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 303 - 343 2e-07 PPR repeat family
TPR_24 PF23276 322 - 429 1.6e-08 Fungal tetratrico peptide repeats
PPR_2 PF13041 334 - 383 1.2e-12 PPR repeat family
PPR PF01535 337 - 366 2.1e-06 PPR repeat
PPR_3 PF13812 392 - 452 1.1e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 406 - 451 9.4e-12 PPR repeat family
PPR PF01535 409 - 437 4.5e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1017
Acc36I ACCTGC 1 cut(s) 39
AccB1I GGYRCC 1 cut(s) 62
AccB7I CCANNNNNTGG 2 cut(s) 72, 309
AccI GTMKAC 2 cut(s) 337, 483
AccII CGCG 2 cut(s) 36, 648
AciI CCGC 2 cut(s) 121, 207
AclI AACGTT 1 cut(s) 755
AclWI GGATC 1 cut(s) 1336
AcsI RAATTY 4 cut(s) 872, 974, 1195, 1417
AcyI GRCGYC 2 cut(s) 63, 204
AfaI GTAC 4 cut(s) 248, 380, 668, 950
AfiI CCNNNNNNNGG 6 cut(s) 72, 199, 309, 529, 1182, 1270
AflIII ACRYGT 1 cut(s) 646
AgsI TTSAA 8 cut(s) 625, 644, 740, 773, 808, 913, 1228, 1390
AjnI CCWGG 1 cut(s) 669
AluBI AGCT 9 cut(s) 147, 388, 432, 445, 491, 783, 880, 1046, 1151
AluI AGCT 9 cut(s) 147, 388, 432, 445, 491, 783, 880, 1046, 1151
Alw21I GWGCWC 2 cut(s) 149, 447
Alw26I GTCTC 3 cut(s) 43, 162, 189
AlwI GGATC 1 cut(s) 1336
AlwNI CAGNNNCTG 1 cut(s) 566
AoxI GGCC 5 cut(s) 260, 267, 426, 517, 893
ApeKI GCWGC 7 cut(s) 123, 299, 349, 388, 780, 783, 1376
ApoI RAATTY 4 cut(s) 872, 974, 1195, 1417
AseI ATTAAT 1 cut(s) 606
Asp700I GAANNNNTTC 1 cut(s) 648
AspLEI GCGC 2 cut(s) 36, 65
AspS9I GGNCC 1 cut(s) 261
AsuHPI GGTGA 3 cut(s) 988, 1108, 1271
BanI GGYRCC 1 cut(s) 62
BanII GRGCYC 3 cut(s) 149, 447, 465
Bbv12I GWGCWC 2 cut(s) 149, 447
BbvI GCAGC 7 cut(s) 110, 311, 361, 375, 770, 792, 1363
BccI CCATC 6 cut(s) 452, 707, 739, 847, 877, 1057
BceAI ACGGC 2 cut(s) 194, 254
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 1 cut(s) 671
BclI TGATCA 1 cut(s) 1318
BcoDI GTCTC 3 cut(s) 43, 162, 189
BfaI CTAG 1 cut(s) 1444
BfoI RGCGCY 1 cut(s) 66
BfuAI ACCTGC 1 cut(s) 39
BglII AGATCT 1 cut(s) 962
BisI GCNGC 8 cut(s) 124, 207, 300, 350, 389, 781, 784, 1377
BlsI GCNGC 8 cut(s) 125, 208, 301, 351, 390, 782, 785, 1378
Bme1390I CCNGG 1 cut(s) 671
BmgT120I GGNCC 1 cut(s) 261
BmiI GGNNCC 1 cut(s) 64
BmrFI CCNGG 1 cut(s) 671
BmsI GCATC 5 cut(s) 359, 387, 671, 1099, 1430
Bpu10I CCTNAGC 1 cut(s) 420
BpuEI CTTGAG 2 cut(s) 308, 1365
BsaHI GRCGYC 2 cut(s) 63, 204
BsaJI CCNNGG 3 cut(s) 264, 670, 1410
BsaXI ACNNNNNCTCC 6 cut(s) 740, 770, 848, 878, 1147, 1177
Bsc4I CCNNNNNNNGG 6 cut(s) 72, 199, 309, 529, 1182, 1270
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 2 cut(s) 359, 1261
Bse3DI GCAATG 1 cut(s) 1299
BseBI CCWGG 1 cut(s) 671
BseDI CCNNGG 3 cut(s) 264, 670, 1410
BseGI GGATG 5 cut(s) 686, 858, 1207, 1404, 1445
BseLI CCNNNNNNNGG 6 cut(s) 72, 199, 309, 529, 1182, 1270
BseMI GCAATG 1 cut(s) 1299
BseMII CTCAG 3 cut(s) 411, 648, 832
BseNI ACTGG 2 cut(s) 359, 1261
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 7 cut(s) 110, 311, 361, 375, 770, 792, 1363
BseYI CCCAGC 1 cut(s) 1151
BsgI GTGCAG 2 cut(s) 430, 598
Bsh1236I CGCG 2 cut(s) 36, 648
BshFI GGCC 5 cut(s) 262, 269, 428, 519, 895
BshNI GGYRCC 1 cut(s) 62
BsiHKAI GWGCWC 2 cut(s) 149, 447
BsiSI CCGG 1 cut(s) 60
BslI CCNNNNNNNGG 6 cut(s) 72, 199, 309, 529, 1182, 1270
BsmAI GTCTC 3 cut(s) 43, 162, 189
BsmBI CGTCTC 2 cut(s) 43, 162
BsmI GAATGC 1 cut(s) 533
BsnI GGCC 5 cut(s) 262, 269, 428, 519, 895
Bsp1286I GDGCHC 3 cut(s) 149, 447, 465
Bsp143I GATC 3 cut(s) 962, 1318, 1341
BspACI CCGC 2 cut(s) 121, 207
BspANI GGCC 5 cut(s) 262, 269, 428, 519, 895
BspCNI CTCAG 3 cut(s) 412, 649, 831
BspFNI CGCG 2 cut(s) 36, 648
BspLI GGNNCC 1 cut(s) 64
BspMI ACCTGC 1 cut(s) 39
BspPI GGATC 1 cut(s) 1336
BspT107I GGYRCC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 1299
BsrFI RCCGGY 1 cut(s) 59
BsrI ACTGG 2 cut(s) 359, 1261
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 3 cut(s) 264, 670, 1410
BssMI GATC 3 cut(s) 962, 1318, 1341
BssNI GRCGYC 2 cut(s) 63, 204
BssT1I CCWWGG 2 cut(s) 264, 1410
Bst2UI CCWGG 1 cut(s) 671
Bst4CI ACNGT 5 cut(s) 475, 544, 691, 1232, 1284
BstACI GRCGYC 2 cut(s) 63, 204
BstC8I GCNNGC 4 cut(s) 413, 426, 430, 434
BstDEI CTNAG 5 cut(s) 420, 657, 818, 903, 1265
BstEII GGTNACC 1 cut(s) 1259
BstENI CCTNNNNNAGG 2 cut(s) 527, 1268
BstF5I GGATG 5 cut(s) 686, 858, 1207, 1404, 1445
BstFNI CGCG 2 cut(s) 36, 648
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 2 cut(s) 36, 65
BstKTI GATC 3 cut(s) 965, 1321, 1344
BstMAI GTCTC 3 cut(s) 43, 162, 189
BstMBI GATC 3 cut(s) 962, 1318, 1341
BstMWI GCNNNNNNNGC 5 cut(s) 299, 397, 421, 442, 516
BstNI CCWGG 1 cut(s) 671
BstPI GGTNACC 1 cut(s) 1259
BstSCI CCNGG 1 cut(s) 669
BstUI CGCG 2 cut(s) 36, 648
BstV1I GCAGC 7 cut(s) 110, 311, 361, 375, 770, 792, 1363
BstX2I RGATCY 1 cut(s) 962
BstYI RGATCY 1 cut(s) 962
BsuRI GGCC 5 cut(s) 262, 269, 428, 519, 895
BtsCI GGATG 5 cut(s) 686, 858, 1207, 1404, 1445
BtsIMutI CAGTG 3 cut(s) 352, 826, 1254
BveI ACCTGC 1 cut(s) 39
Cac8I GCNNGC 4 cut(s) 413, 426, 430, 434
CaiI CAGNNNCTG 1 cut(s) 566
CfoI GCGC 2 cut(s) 36, 65
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 1 cut(s) 261
CseI GACGC 2 cut(s) 25, 212
Csp6I GTAC 4 cut(s) 247, 379, 667, 949
CviAII CATG 4 cut(s) 448, 1207, 1339, 1366
CviQI GTAC 4 cut(s) 247, 379, 667, 949
DdeI CTNAG 5 cut(s) 420, 657, 818, 903, 1265
DinI GGCGCC 1 cut(s) 64
DpnI GATC 3 cut(s) 964, 1320, 1343
DpnII GATC 3 cut(s) 962, 1318, 1341
Ecl136II GAGCTC 2 cut(s) 147, 445
Eco130I CCWWGG 2 cut(s) 264, 1410
Eco24I GRGCYC 3 cut(s) 149, 447, 465
Eco53kI GAGCTC 2 cut(s) 147, 445
Eco91I GGTNACC 1 cut(s) 1259
EcoICRI GAGCTC 2 cut(s) 147, 445
EcoNI CCTNNNNNAGG 2 cut(s) 527, 1268
EcoO65I GGTNACC 1 cut(s) 1259
EcoRI GAATTC 1 cut(s) 1195
EcoRII CCWGG 1 cut(s) 669
EcoT14I CCWWGG 2 cut(s) 264, 1410
EcoT38I GRGCYC 3 cut(s) 149, 447, 465
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 2 cut(s) 264, 1410
Esp3I CGTCTC 2 cut(s) 43, 162
FaeI CATG 4 cut(s) 451, 1210, 1342, 1369
FatI CATG 4 cut(s) 447, 1206, 1338, 1365
FbaI TGATCA 1 cut(s) 1318
FblI GTMKAC 2 cut(s) 337, 483
Fnu4HI GCNGC 8 cut(s) 124, 207, 300, 350, 389, 781, 784, 1377
FokI GGATG 4 cut(s) 693, 865, 1214, 1411
FriOI GRGCYC 3 cut(s) 149, 447, 465
Fsp4HI GCNGC 8 cut(s) 124, 207, 300, 350, 389, 781, 784, 1377
FspBI CTAG 1 cut(s) 1444
GlaI GCGC 2 cut(s) 35, 64
GluI GCNGC 8 cut(s) 124, 207, 300, 350, 389, 781, 784, 1377
GsaI CCCAGC 1 cut(s) 1155
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 5 cut(s) 262, 269, 428, 519, 895
HapII CCGG 1 cut(s) 60
HgaI GACGC 2 cut(s) 25, 212
HhaI GCGC 2 cut(s) 36, 65
Hin1I GRCGYC 2 cut(s) 63, 204
Hin1II CATG 4 cut(s) 451, 1210, 1342, 1369
Hin6I GCGC 2 cut(s) 34, 63
HinP1I GCGC 2 cut(s) 34, 63
HincII GTYRAC 2 cut(s) 11, 759
HindII GTYRAC 2 cut(s) 11, 759
HinfI GANTC 2 cut(s) 178, 1172
HpaII CCGG 1 cut(s) 60
HphI GGTGA 3 cut(s) 988, 1108, 1271
Hpy166II GTNNAC 5 cut(s) 11, 338, 381, 484, 759
Hpy188I TCNGA 7 cut(s) 91, 151, 162, 197, 568, 1079, 1201
Hpy188III TCNNGA 7 cut(s) 40, 284, 325, 632, 791, 919, 1382
Hpy8I GTNNAC 5 cut(s) 11, 338, 381, 484, 759
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 3 cut(s) 160, 797, 931
HpyCH4III ACNGT 5 cut(s) 475, 544, 691, 1232, 1284
HpyCH4IV ACGT 4 cut(s) 80, 377, 582, 755
HpyCH4V TGCA 5 cut(s) 299, 411, 573, 615, 684
HpyF10VI GCNNNNNNNGC 5 cut(s) 299, 397, 421, 442, 516
HpyF3I CTNAG 5 cut(s) 420, 657, 818, 903, 1265
HpySE526I ACGT 4 cut(s) 80, 377, 582, 755
Hsp92I GRCGYC 2 cut(s) 63, 204
Hsp92II CATG 4 cut(s) 451, 1210, 1342, 1369
HspAI GCGC 2 cut(s) 34, 63
KasI GGCGCC 1 cut(s) 62
Ksp22I TGATCA 1 cut(s) 1318
Kzo9I GATC 3 cut(s) 962, 1318, 1341
LmnI GCTCC 4 cut(s) 4, 144, 152, 169
Lsp1109I GCAGC 7 cut(s) 110, 311, 361, 375, 770, 792, 1363
LweI GCATC 5 cut(s) 359, 387, 671, 1099, 1430
MaeI CTAG 1 cut(s) 1444
MaeII ACGT 4 cut(s) 80, 377, 582, 755
MaeIII GTNAC 2 cut(s) 1009, 1259
MalI GATC 3 cut(s) 964, 1320, 1343
MboI GATC 3 cut(s) 962, 1318, 1341
MboII GAAGA 4 cut(s) 187, 994, 1072, 1092
MflI RGATCY 1 cut(s) 962
MhlI GDGCHC 3 cut(s) 149, 447, 465
MluCI AATT 9 cut(s) 607, 708, 808, 832, 872, 974, 1026, 1195, 1417
MluI ACGCGT 1 cut(s) 646
Mly113I GGCGCC 1 cut(s) 63
MmeI TCCRAC 1 cut(s) 174
MroXI GAANNNNTTC 1 cut(s) 648
MseI TTAA 3 cut(s) 56, 606, 924
MslI CAYNNNNRTG 1 cut(s) 357
MspA1I CMGCKG 3 cut(s) 123, 783, 880
MspI CCGG 1 cut(s) 60
MspR9I CCNGG 1 cut(s) 671
Mva1269I GAATGC 1 cut(s) 533
MvaI CCWGG 1 cut(s) 671
MvnI CGCG 2 cut(s) 36, 648
MwoI GCNNNNNNNGC 5 cut(s) 299, 397, 421, 442, 516
NarI GGCGCC 1 cut(s) 63
NdeII GATC 3 cut(s) 962, 1318, 1341
NlaIII CATG 4 cut(s) 451, 1210, 1342, 1369
NlaIV GGNNCC 1 cut(s) 64
NmeAIII GCCGAG 1 cut(s) 390
NmuCI GTSAC 1 cut(s) 1259
PctI GAATGC 1 cut(s) 533
PdmI GAANNNNTTC 1 cut(s) 648
PfeI GAWTC 2 cut(s) 178, 1172
PflMI CCANNNNNTGG 2 cut(s) 72, 309
PkrI GCNGC 8 cut(s) 125, 208, 301, 351, 390, 782, 785, 1378
PluTI GGCGCC 1 cut(s) 66
PshBI ATTAAT 1 cut(s) 606
PsiI TTATAA 1 cut(s) 1017
Psp124BI GAGCTC 2 cut(s) 149, 447
Psp1406I AACGTT 1 cut(s) 755
Psp6I CCWGG 1 cut(s) 669
PspEI GGTNACC 1 cut(s) 1259
PspFI CCCAGC 1 cut(s) 1151
PspGI CCWGG 1 cut(s) 669
PspN4I GGNNCC 1 cut(s) 64
PspPI GGNCC 1 cut(s) 261
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 962
PvuII CAGCTG 2 cut(s) 783, 880
RsaI GTAC 4 cut(s) 248, 380, 668, 950
RsaNI GTAC 4 cut(s) 247, 379, 667, 949
RseI CAYNNNNRTG 1 cut(s) 357
SacI GAGCTC 2 cut(s) 149, 447
SaqAI TTAA 3 cut(s) 56, 606, 924
SatI GCNGC 8 cut(s) 124, 207, 300, 350, 389, 781, 784, 1377
Sau3AI GATC 3 cut(s) 962, 1318, 1341
Sau96I GGNCC 1 cut(s) 261
ScrFI CCNGG 1 cut(s) 671
SduI GDGCHC 3 cut(s) 149, 447, 465
SfaNI GCATC 5 cut(s) 359, 387, 671, 1099, 1430
SfoI GGCGCC 1 cut(s) 64
SmiMI CAYNNNNRTG 1 cut(s) 357
SmlI CTYRAG 2 cut(s) 323, 1380
SmoI CTYRAG 2 cut(s) 323, 1380
Sse9I AATT 9 cut(s) 607, 708, 808, 832, 872, 974, 1026, 1195, 1417
SsiI CCGC 2 cut(s) 121, 207
SspDI GGCGCC 1 cut(s) 62
SspI AATATT 2 cut(s) 1021, 1054
SspMI CTAG 1 cut(s) 1444
SstI GAGCTC 2 cut(s) 149, 447
StyD4I CCNGG 1 cut(s) 669
StyI CCWWGG 2 cut(s) 264, 1410
TaaI ACNGT 5 cut(s) 475, 544, 691, 1232, 1284
TaiI ACGT 4 cut(s) 83, 380, 585, 758
TaqI TCGA 2 cut(s) 41, 137
TasI AATT 9 cut(s) 607, 708, 808, 832, 872, 974, 1026, 1195, 1417
TatI WGTACW 1 cut(s) 246
TauI GCSGC 1 cut(s) 209
TfiI GAWTC 2 cut(s) 178, 1172
Tru1I TTAA 3 cut(s) 56, 606, 924
Tru9I TTAA 3 cut(s) 56, 606, 924
TscAI CASTG 3 cut(s) 359, 826, 1261
TseFI GTSAC 1 cut(s) 1259
TseI GCWGC 7 cut(s) 123, 299, 349, 388, 780, 783, 1376
Tsp45I GTSAC 1 cut(s) 1259
TspDTI ATGAA 8 cut(s) 569, 885, 995, 1100, 1115, 1310, 1371, 1382
TspGWI ACGGA 3 cut(s) 119, 157, 215
TspRI CASTG 3 cut(s) 359, 826, 1261
Van91I CCANNNNNTGG 2 cut(s) 72, 309
VspI ATTAAT 1 cut(s) 606
XagI CCTNNNNNAGG 2 cut(s) 527, 1268
XapI RAATTY 4 cut(s) 872, 974, 1195, 1417
XcmI CCANNNNNNNNNTGG 1 cut(s) 271
XmiI GTMKAC 2 cut(s) 337, 483
XmnI GAANNNNTTC 1 cut(s) 648
XspI CTAG 1 cut(s) 1444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.