MD02G1306000.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004347
Physical Location & Seq
Forward (+)
35900856 .. 35902699
1844 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1306000.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGGAGCGTCAACTCCTTTATCCCACTAACCTGCGCGTCTCGACCTCAACCTCCTTAACCGGCGCCACATATTGGACGACGTTCTACTCTGAACACACCCACAAACCAAACCCAACAATCCGCTGCCTCCGTTTATCGAAACGGAGCTCCGACCCGTCTCTGACGAAGGAGCAGAAGAATCTGTCTCAACTCCTCCGAACGGACGCCGCCGTGAGAAACATTGAGAGGAAAGCCAACTCCAAGAAGTACAACAACCTTTGGCCCAAAGCCGTTTTGGAGGCTCTGGACGAGGCTATTGCAGCCAATCTCTGGGAAACTGCTCTCAAGCACTGGTATGAGCCGAGATGCCAAACGTACACCAAGCTGCTGATGATGCTTGGCAAGTGCAGGCAACCTGAGCAGGCCAGCTTGCTTTTTGAGCTCATGTTGAGTGATGGGCTCAAACCTACTGTTGATGTCTACACAGCTCTTGTTAGTGTTTATGGCAAAAGTGGCCTCCTTGACAAGGCATTCTCTACCGTCGATGATATGAAGTCAGTTTCTGACTGCAAACCAGACGTATATACATATTCTATTCTTATTAATTCCTGCACCAAGTTCAATCGTCCTGACCTGATTGAACGAGTTCTTGCTGAGATGTCGTACCTGGGGATTGGATGCAACACAGTCATATACAATACCCTAATTGATGGATATGGTAAGGCTGAAATGTTTGAACTGATGGAGGAAACGTTGACAAATATGATTGAAAGTGGCAGCTGCCTTCCTGATGTTTTCACTTTCAATTCTTTTCTCAGTGCTTATGGGAATTGTGGGCAGACAGAGAAGATGGAGAAGTGGTATGATGAATTTCAGCTGATGGGAATAAGGCCAGACCCTAAGACATTCAATATCCTGATTAAATCATACGGGAAGGCAAGAATGTACGAAAAGATGAGATCTGTTATGGAATTTATGGAGAAAAGGTTTTTCACCCCAACTATTGTTACTTATAATATTGTAATTGAGGTATTCGGTAAAGCTGGAAATATTGAGAAGATGGACGAATACTTCCGAAGAATGAAGCATCAAGGAATGAAGCCCAACTCTATCACCTATTGTTCTCTTGGTAGTGCGTATAGCAAAGCTGGGAGTATAAGTAAAGTTGATTCCATTTTGAGGCAAGTAGAGAATTCGGATGTCATGTTAGATACACCTTTTTTTCAACTGTATTATTAG

Protein Analysis

406

Amino Acids

46.52

Weight (kDa)

8.39

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 145 - 178 4.3e-06 PPR repeat
PPR_2 PF13041 149 - 199 1.7e-12 PPR repeat family
PPR_3 PF13812 208 - 270 4.3e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 221 - 261 4e-06 PPR repeat family
PPR_2 PF13041 256 - 304 4.9e-11 PPR repeat family
PPR_3 PF13812 282 - 340 1.1e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 295 - 335 6e-07 PPR repeat family
PPR_2 PF13041 326 - 373 1.3e-11 PPR repeat family
PPR PF01535 329 - 358 1.7e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 993
Acc36I ACCTGC 1 cut(s) 39
AccB1I GGYRCC 1 cut(s) 62
AccB7I CCANNNNNTGG 2 cut(s) 72, 309
AccI GTMKAC 1 cut(s) 459
AccII CGCG 1 cut(s) 36
AciI CCGC 2 cut(s) 121, 207
AclI AACGTT 1 cut(s) 731
AcsI RAATTY 3 cut(s) 848, 950, 1171
AcyI GRCGYC 2 cut(s) 63, 204
AfaI GTAC 4 cut(s) 248, 356, 644, 926
AfiI CCNNNNNNNGG 5 cut(s) 72, 199, 309, 505, 1158
AgsI TTSAA 7 cut(s) 601, 620, 716, 749, 784, 889, 1205
AjnI CCWGG 1 cut(s) 645
AluBI AGCT 9 cut(s) 147, 364, 408, 421, 467, 759, 856, 1022, 1127
AluI AGCT 9 cut(s) 147, 364, 408, 421, 467, 759, 856, 1022, 1127
Alw21I GWGCWC 2 cut(s) 149, 423
Alw26I GTCTC 3 cut(s) 43, 162, 189
AlwNI CAGNNNCTG 1 cut(s) 542
AoxI GGCC 4 cut(s) 260, 402, 493, 869
ApeKI GCWGC 5 cut(s) 123, 299, 364, 756, 759
ApoI RAATTY 3 cut(s) 848, 950, 1171
AseI ATTAAT 1 cut(s) 582
Asp700I GAANNNNTTC 1 cut(s) 624
AspLEI GCGC 2 cut(s) 36, 65
AspS9I GGNCC 1 cut(s) 261
AsuHPI GGTGA 2 cut(s) 964, 1084
BanI GGYRCC 1 cut(s) 62
BanII GRGCYC 3 cut(s) 149, 423, 441
Bbv12I GWGCWC 2 cut(s) 149, 423
BbvI GCAGC 5 cut(s) 110, 311, 351, 746, 768
BccI CCATC 6 cut(s) 428, 683, 715, 823, 853, 1033
BceAI ACGGC 2 cut(s) 194, 254
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 1 cut(s) 647
BcoDI GTCTC 3 cut(s) 43, 162, 189
BfoI RGCGCY 1 cut(s) 66
BfuAI ACCTGC 1 cut(s) 39
BglII AGATCT 1 cut(s) 938
BisI GCNGC 6 cut(s) 124, 207, 300, 365, 757, 760
BlsI GCNGC 6 cut(s) 125, 208, 301, 366, 758, 761
Bme1390I CCNGG 1 cut(s) 647
BmgT120I GGNCC 1 cut(s) 261
BmiI GGNNCC 1 cut(s) 64
BmrFI CCNGG 1 cut(s) 647
BmsI GCATC 4 cut(s) 335, 363, 647, 1075
Bpu10I CCTNAGC 1 cut(s) 396
BpuEI CTTGAG 1 cut(s) 308
BsaHI GRCGYC 2 cut(s) 63, 204
BsaJI CCNNGG 1 cut(s) 646
BsaXI ACNNNNNCTCC 8 cut(s) 716, 746, 824, 854, 950, 980, 1123, 1153
Bsc4I CCNNNNNNNGG 5 cut(s) 72, 199, 309, 505, 1158
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 1 cut(s) 335
BseBI CCWGG 1 cut(s) 647
BseDI CCNNGG 1 cut(s) 646
BseGI GGATG 2 cut(s) 662, 1183
BseLI CCNNNNNNNGG 5 cut(s) 72, 199, 309, 505, 1158
BseMII CTCAG 3 cut(s) 387, 624, 808
BseNI ACTGG 1 cut(s) 335
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 5 cut(s) 110, 311, 351, 746, 768
BseYI CCCAGC 1 cut(s) 1127
BsgI GTGCAG 2 cut(s) 406, 574
Bsh1236I CGCG 1 cut(s) 36
BshFI GGCC 4 cut(s) 262, 404, 495, 871
BshNI GGYRCC 1 cut(s) 62
BsiHKAI GWGCWC 2 cut(s) 149, 423
BsiSI CCGG 1 cut(s) 60
BslI CCNNNNNNNGG 5 cut(s) 72, 199, 309, 505, 1158
BsmAI GTCTC 3 cut(s) 43, 162, 189
BsmBI CGTCTC 2 cut(s) 43, 162
BsmI GAATGC 1 cut(s) 509
BsnI GGCC 4 cut(s) 262, 404, 495, 871
Bsp1286I GDGCHC 3 cut(s) 149, 423, 441
Bsp143I GATC 1 cut(s) 938
BspACI CCGC 2 cut(s) 121, 207
BspANI GGCC 4 cut(s) 262, 404, 495, 871
BspCNI CTCAG 3 cut(s) 388, 625, 807
BspFNI CGCG 1 cut(s) 36
BspLI GGNNCC 1 cut(s) 64
BspMI ACCTGC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 62
BsrFI RCCGGY 1 cut(s) 59
BsrI ACTGG 1 cut(s) 335
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 1 cut(s) 646
BssMI GATC 1 cut(s) 938
BssNI GRCGYC 2 cut(s) 63, 204
Bst2UI CCWGG 1 cut(s) 647
Bst4CI ACNGT 4 cut(s) 451, 520, 667, 1209
BstACI GRCGYC 2 cut(s) 63, 204
BstC8I GCNNGC 4 cut(s) 389, 402, 406, 410
BstDEI CTNAG 4 cut(s) 396, 633, 794, 879
BstENI CCTNNNNNAGG 1 cut(s) 503
BstF5I GGATG 2 cut(s) 662, 1183
BstFNI CGCG 1 cut(s) 36
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 2 cut(s) 36, 65
BstKTI GATC 1 cut(s) 941
BstMAI GTCTC 3 cut(s) 43, 162, 189
BstMBI GATC 1 cut(s) 938
BstMWI GCNNNNNNNGC 5 cut(s) 299, 373, 397, 418, 492
BstNI CCWGG 1 cut(s) 647
BstSCI CCNGG 1 cut(s) 645
BstUI CGCG 1 cut(s) 36
BstV1I GCAGC 5 cut(s) 110, 311, 351, 746, 768
BstX2I RGATCY 1 cut(s) 938
BstYI RGATCY 1 cut(s) 938
BsuRI GGCC 4 cut(s) 262, 404, 495, 871
BtsCI GGATG 2 cut(s) 662, 1183
BtsIMutI CAGTG 2 cut(s) 328, 802
BveI ACCTGC 1 cut(s) 39
Cac8I GCNNGC 4 cut(s) 389, 402, 406, 410
CaiI CAGNNNCTG 1 cut(s) 542
CfoI GCGC 2 cut(s) 36, 65
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 1 cut(s) 261
CseI GACGC 2 cut(s) 25, 212
Csp6I GTAC 4 cut(s) 247, 355, 643, 925
CviAII CATG 2 cut(s) 424, 1183
CviQI GTAC 4 cut(s) 247, 355, 643, 925
DdeI CTNAG 4 cut(s) 396, 633, 794, 879
DinI GGCGCC 1 cut(s) 64
DpnI GATC 1 cut(s) 940
DpnII GATC 1 cut(s) 938
Ecl136II GAGCTC 2 cut(s) 147, 421
Eco24I GRGCYC 3 cut(s) 149, 423, 441
Eco53kI GAGCTC 2 cut(s) 147, 421
EcoICRI GAGCTC 2 cut(s) 147, 421
EcoNI CCTNNNNNAGG 1 cut(s) 503
EcoRI GAATTC 1 cut(s) 1171
EcoRII CCWGG 1 cut(s) 645
EcoT38I GRGCYC 3 cut(s) 149, 423, 441
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
Esp3I CGTCTC 2 cut(s) 43, 162
FaeI CATG 2 cut(s) 427, 1186
FatI CATG 2 cut(s) 423, 1182
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 6 cut(s) 124, 207, 300, 365, 757, 760
FokI GGATG 2 cut(s) 669, 1190
FriOI GRGCYC 3 cut(s) 149, 423, 441
Fsp4HI GCNGC 6 cut(s) 124, 207, 300, 365, 757, 760
GlaI GCGC 2 cut(s) 35, 64
GluI GCNGC 6 cut(s) 124, 207, 300, 365, 757, 760
GsaI CCCAGC 1 cut(s) 1131
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 4 cut(s) 262, 404, 495, 871
HapII CCGG 1 cut(s) 60
HgaI GACGC 2 cut(s) 25, 212
HhaI GCGC 2 cut(s) 36, 65
Hin1I GRCGYC 2 cut(s) 63, 204
Hin1II CATG 2 cut(s) 427, 1186
Hin6I GCGC 2 cut(s) 34, 63
HinP1I GCGC 2 cut(s) 34, 63
HincII GTYRAC 2 cut(s) 11, 735
HindII GTYRAC 2 cut(s) 11, 735
HinfI GANTC 2 cut(s) 178, 1148
HpaII CCGG 1 cut(s) 60
HphI GGTGA 2 cut(s) 964, 1084
Hpy166II GTNNAC 4 cut(s) 11, 357, 460, 735
Hpy188I TCNGA 7 cut(s) 91, 151, 162, 197, 544, 1055, 1177
Hpy188III TCNNGA 5 cut(s) 40, 284, 608, 767, 895
Hpy8I GTNNAC 4 cut(s) 11, 357, 460, 735
Hpy99I CGWCG 2 cut(s) 82, 524
HpyAV CCTTC 3 cut(s) 160, 773, 907
HpyCH4III ACNGT 4 cut(s) 451, 520, 667, 1209
HpyCH4IV ACGT 4 cut(s) 80, 353, 558, 731
HpyCH4V TGCA 5 cut(s) 299, 387, 549, 591, 660
HpyF10VI GCNNNNNNNGC 5 cut(s) 299, 373, 397, 418, 492
HpyF3I CTNAG 4 cut(s) 396, 633, 794, 879
HpySE526I ACGT 4 cut(s) 80, 353, 558, 731
Hsp92I GRCGYC 2 cut(s) 63, 204
Hsp92II CATG 2 cut(s) 427, 1186
HspAI GCGC 2 cut(s) 34, 63
KasI GGCGCC 1 cut(s) 62
Kzo9I GATC 1 cut(s) 938
LmnI GCTCC 4 cut(s) 4, 144, 152, 169
Lsp1109I GCAGC 5 cut(s) 110, 311, 351, 746, 768
LweI GCATC 4 cut(s) 335, 363, 647, 1075
MaeII ACGT 4 cut(s) 80, 353, 558, 731
MaeIII GTNAC 1 cut(s) 985
MalI GATC 1 cut(s) 940
MboI GATC 1 cut(s) 938
MboII GAAGA 4 cut(s) 187, 838, 1048, 1068
MflI RGATCY 1 cut(s) 938
MhlI GDGCHC 3 cut(s) 149, 423, 441
MluCI AATT 8 cut(s) 583, 684, 784, 808, 848, 950, 1002, 1171
Mly113I GGCGCC 1 cut(s) 63
MmeI TCCRAC 1 cut(s) 174
MroXI GAANNNNTTC 1 cut(s) 624
MseI TTAA 3 cut(s) 56, 582, 900
MslI CAYNNNNRTG 1 cut(s) 333
MspA1I CMGCKG 3 cut(s) 123, 759, 856
MspI CCGG 1 cut(s) 60
MspR9I CCNGG 1 cut(s) 647
Mva1269I GAATGC 1 cut(s) 509
MvaI CCWGG 1 cut(s) 647
MvnI CGCG 1 cut(s) 36
MwoI GCNNNNNNNGC 5 cut(s) 299, 373, 397, 418, 492
NarI GGCGCC 1 cut(s) 63
NdeII GATC 1 cut(s) 938
NlaIII CATG 2 cut(s) 427, 1186
NlaIV GGNNCC 1 cut(s) 64
NmeAIII GCCGAG 1 cut(s) 366
PctI GAATGC 1 cut(s) 509
PdmI GAANNNNTTC 1 cut(s) 624
PfeI GAWTC 2 cut(s) 178, 1148
PflMI CCANNNNNTGG 2 cut(s) 72, 309
PkrI GCNGC 6 cut(s) 125, 208, 301, 366, 758, 761
PluTI GGCGCC 1 cut(s) 66
PshBI ATTAAT 1 cut(s) 582
PsiI TTATAA 1 cut(s) 993
Psp124BI GAGCTC 2 cut(s) 149, 423
Psp1406I AACGTT 1 cut(s) 731
Psp6I CCWGG 1 cut(s) 645
PspFI CCCAGC 1 cut(s) 1127
PspGI CCWGG 1 cut(s) 645
PspN4I GGNNCC 1 cut(s) 64
PspPI GGNCC 1 cut(s) 261
PstNI CAGNNNCTG 1 cut(s) 542
PsuI RGATCY 1 cut(s) 938
PvuII CAGCTG 2 cut(s) 759, 856
RsaI GTAC 4 cut(s) 248, 356, 644, 926
RsaNI GTAC 4 cut(s) 247, 355, 643, 925
RseI CAYNNNNRTG 1 cut(s) 333
SacI GAGCTC 2 cut(s) 149, 423
SaqAI TTAA 3 cut(s) 56, 582, 900
SatI GCNGC 6 cut(s) 124, 207, 300, 365, 757, 760
Sau3AI GATC 1 cut(s) 938
Sau96I GGNCC 1 cut(s) 261
ScrFI CCNGG 1 cut(s) 647
SduI GDGCHC 3 cut(s) 149, 423, 441
SfaNI GCATC 4 cut(s) 335, 363, 647, 1075
SfoI GGCGCC 1 cut(s) 64
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
Sse9I AATT 8 cut(s) 583, 684, 784, 808, 848, 950, 1002, 1171
SsiI CCGC 2 cut(s) 121, 207
SspDI GGCGCC 1 cut(s) 62
SspI AATATT 2 cut(s) 997, 1030
SstI GAGCTC 2 cut(s) 149, 423
StyD4I CCNGG 1 cut(s) 645
TaaI ACNGT 4 cut(s) 451, 520, 667, 1209
TaiI ACGT 4 cut(s) 83, 356, 561, 734
TaqI TCGA 3 cut(s) 41, 137, 522
TasI AATT 8 cut(s) 583, 684, 784, 808, 848, 950, 1002, 1171
TatI WGTACW 1 cut(s) 246
TauI GCSGC 1 cut(s) 209
TfiI GAWTC 2 cut(s) 178, 1148
Tru1I TTAA 3 cut(s) 56, 582, 900
Tru9I TTAA 3 cut(s) 56, 582, 900
TscAI CASTG 2 cut(s) 335, 802
TseI GCWGC 5 cut(s) 123, 299, 364, 756, 759
TspDTI ATGAA 4 cut(s) 545, 861, 1076, 1091
TspGWI ACGGA 3 cut(s) 119, 157, 215
TspRI CASTG 2 cut(s) 335, 802
Van91I CCANNNNNTGG 2 cut(s) 72, 309
VspI ATTAAT 1 cut(s) 582
XagI CCTNNNNNAGG 1 cut(s) 503
XapI RAATTY 3 cut(s) 848, 950, 1171
XcmI CCANNNNNNNNNTGG 1 cut(s) 271
XmiI GTMKAC 1 cut(s) 459
XmnI GAANNNNTTC 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.