AT3G54130

Josephin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0016089
Physical Location & Seq
Reverse (-)
20042641 .. 20044580
1940 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G54130.1

Sequence Viewer

Length: 843 bp
ATGGAACGAACAAGCAACGGAGGAATGTTGTATCACGAGGTTCAAGAGTCAAATCTGTGCGCCGTACACTGCGTCAACACGGTGTTGCAAGGGCCTTTCTTCTCTGAATTCGATTTGGCTGCCGTCGCCGCCGATCTAGACGGGAAGGAGCGTCAGGTTATGCTTGAAGGTGCTGCCGTTGGAGGTTTTGCTCCCGGCGATTTCTTAGCTGAAGAGTCGCACAATGTTTCCCTCGGCGGCGATTTTAGTATCCAGGTCTTGCAAAAAGCTTTGGAAGTGTGGGATCTACAAGTAATTCCACTCAATTGTCCAGATGCAGAGCCTGCGCAGATAGACCCTGAGCTTGAAAGCGCCTTTATCTGCCACTTGCATGACCATTGGTTTTGTATAAGGAAAGTGAATGGCGAATGGTATAACTTTGATAGTCTCCTTGCTGCCCCGCAACATTTATCGAAGTTCTACCTTTCTGCGTTCTTAGACTCTCTCAAAGGCGCAGGATGGAGTATCTTCATAGTGAAAGGAAACTTCCCTCAGGAATGTCCCATGTCGTCTTCTTCTGAAGCTTCCAATAGTTTCGGGCAGTGGCTTTCTCCTGAAGACGCAGAGAGAATAAGAAAGAACACGAGCTCAGGTTCTTCTGCCAGAAACAAGAGAAGTAATGACAATGTTAATCAACAAAGACGGAATCAGGCATTGTCACGTGAGGAAGTGCAGGCTTTTTCAGAGATGGAAGATGATGACTTGAAGGCAGCAATTGCAGCAAGTTTGCTTGATGCTTCTGCTGCTGAGGCCAATCTCGGAGCTGTGGGCACTTCTGAAAAGGAAACTGAGAAACAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Josephin

Protein Analysis

280

Amino Acids

30.69

Weight (kDa)

4.64

Isoelectric Point (pI)

46.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Josephin PF02099 15 - 173 2.6e-54 Josephin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54130
fragaria_vesca FvH4_6g12170
malus_domestica MD04G1146200.v1.1 MD12G1159400.v1.1
prunus_persica Prupe.6G269800_v2.0.a1
pyrus_communis pycom04g13180
rosa_chinensis RchiOBHm_Chr3g0463971
rosa_laevigata RLG00000024731
rosa_multiflora Rmu_sc0006492.1_g000007
rosa_roxburghii Rroxscaffold_6G00416100
rosa_rugosa Rorug03G0069700
rosa_samantha Rh3AG126600 Rh3BG130800 Rh3DG132000
rosa_wichuraiana Rw3G010690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 327
AciI CCGC 3 cut(s) 129, 237, 440
AclWI GGATC 1 cut(s) 291
AcsI RAATTY 1 cut(s) 107
AcuI CTGAAG 3 cut(s) 231, 579, 615
AcvI CACGTG 1 cut(s) 701
AfaI GTAC 1 cut(s) 66
AgsI TTSAA 4 cut(s) 44, 167, 347, 745
AjnI CCWGG 1 cut(s) 252
AluBI AGCT 6 cut(s) 209, 269, 343, 563, 627, 803
AluI AGCT 6 cut(s) 209, 269, 343, 563, 627, 803
Alw21I GWGCWC 1 cut(s) 629
Alw26I GTCTC 1 cut(s) 431
AlwI GGATC 1 cut(s) 291
AlwNI CAGNNNCTG 1 cut(s) 323
AoxI GGCC 2 cut(s) 92, 789
ApeKI GCWGC 6 cut(s) 119, 173, 434, 749, 758, 782
ApoI RAATTY 1 cut(s) 107
AspLEI GCGC 4 cut(s) 62, 328, 353, 494
AspS9I GGNCC 1 cut(s) 92
AsuC2I CCSGG 1 cut(s) 195
AxyI CCTNAGG 1 cut(s) 531
BaeGI GKGCMC 1 cut(s) 812
BanII GRGCYC 1 cut(s) 629
BauI CACGAG 2 cut(s) 35, 622
BbrPI CACGTG 1 cut(s) 701
BbsI GAAGAC 2 cut(s) 543, 603
Bbv12I GWGCWC 1 cut(s) 629
BbvCI CCTCAGC 1 cut(s) 786
BbvI GCAGC 6 cut(s) 106, 160, 421, 761, 769, 770
BccI CCATC 2 cut(s) 492, 721
BceAI ACGGC 3 cut(s) 47, 107, 161
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 1 cut(s) 254
BciVI GTATCC 1 cut(s) 260
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 431
BfaI CTAG 1 cut(s) 137
BfoI RGCGCY 1 cut(s) 354
BfuI GTATCC 1 cut(s) 260
BisI GCNGC 8 cut(s) 120, 129, 174, 238, 435, 750, 759, 783
BlsI GCNGC 8 cut(s) 121, 130, 175, 239, 436, 751, 760, 784
Bme1390I CCNGG 2 cut(s) 195, 254
BmgT120I GGNCC 1 cut(s) 92
BmrFI CCNGG 2 cut(s) 195, 254
BmsI GCATC 2 cut(s) 304, 763
BpiI GAAGAC 2 cut(s) 543, 603
Bpu10I CCTNAGC 3 cut(s) 339, 628, 786
BpuMI CCSGG 1 cut(s) 195
BsaAI YACGTR 1 cut(s) 701
BsaJI CCNNGG 1 cut(s) 232
Bse21I CCTNAGG 1 cut(s) 531
BseBI CCWGG 1 cut(s) 254
BseDI CCNNGG 1 cut(s) 232
BseGI GGATG 1 cut(s) 503
BseMII CTCAG 5 cut(s) 330, 545, 642, 777, 819
BseSI GKGCMC 1 cut(s) 812
BseXI GCAGC 6 cut(s) 106, 160, 421, 761, 769, 770
BsgI GTGCAG 1 cut(s) 731
BshFI GGCC 2 cut(s) 94, 791
BsiHKAI GWGCWC 1 cut(s) 629
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 1 cut(s) 525
BsmAI GTCTC 1 cut(s) 431
BsmFI GGGAC 1 cut(s) 525
BsnI GGCC 2 cut(s) 94, 791
Bsp1286I GDGCHC 2 cut(s) 629, 812
Bsp143I GATC 2 cut(s) 133, 283
BspACI CCGC 3 cut(s) 129, 237, 440
BspANI GGCC 2 cut(s) 94, 791
BspCNI CTCAG 5 cut(s) 331, 544, 641, 778, 820
BspPI GGATC 1 cut(s) 291
BssECI CCNNGG 1 cut(s) 232
BssMI GATC 2 cut(s) 133, 283
BssSI CACGAG 2 cut(s) 35, 622
Bst2BI CACGAG 2 cut(s) 35, 622
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 1 cut(s) 82
Bst6I CTCTTC 1 cut(s) 207
BstAPI GCANNNNNTGC 2 cut(s) 323, 755
BstBAI YACGTR 1 cut(s) 701
BstC8I GCNNGC 2 cut(s) 324, 714
BstDEI CTNAG 7 cut(s) 205, 339, 475, 531, 628, 786, 828
BstF5I GGATG 1 cut(s) 503
BstH2I RGCGCY 1 cut(s) 354
BstHHI GCGC 4 cut(s) 62, 328, 353, 494
BstKTI GATC 2 cut(s) 136, 286
BstMAI GTCTC 1 cut(s) 431
BstMBI GATC 2 cut(s) 133, 283
BstMWI GCNNNNNNNGC 7 cut(s) 125, 128, 323, 755, 758, 782, 788
BstNI CCWGG 1 cut(s) 254
BstSCI CCNGG 2 cut(s) 193, 252
BstSLI GKGCMC 1 cut(s) 812
BstV1I GCAGC 6 cut(s) 106, 160, 421, 761, 769, 770
BstV2I GAAGAC 2 cut(s) 543, 603
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
Bsu36I CCTNAGG 1 cut(s) 531
BsuI GTATCC 1 cut(s) 260
BsuRI GGCC 2 cut(s) 94, 791
BtsCI GGATG 1 cut(s) 503
BtsI GCAGTG 2 cut(s) 67, 587
BtsIMutI CAGTG 2 cut(s) 67, 587
Cac8I GCNNGC 2 cut(s) 324, 714
CaiI CAGNNNCTG 1 cut(s) 323
CfoI GCGC 4 cut(s) 62, 328, 353, 494
Cfr13I GGNCC 1 cut(s) 92
CseI GACGC 3 cut(s) 61, 140, 608
Csp6I GTAC 1 cut(s) 65
CviAII CATG 2 cut(s) 371, 544
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 7 cut(s) 205, 339, 475, 531, 628, 786, 828
DpnI GATC 2 cut(s) 135, 285
DpnII GATC 2 cut(s) 133, 283
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Ecl136II GAGCTC 1 cut(s) 627
Eco24I GRGCYC 1 cut(s) 629
Eco53kI GAGCTC 1 cut(s) 627
Eco57I CTGAAG 3 cut(s) 231, 579, 615
Eco72I CACGTG 1 cut(s) 701
Eco81I CCTNAGG 1 cut(s) 531
EcoICRI GAGCTC 1 cut(s) 627
EcoO109I RGGNCCY 1 cut(s) 92
EcoRI GAATTC 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 252
EcoT38I GRGCYC 1 cut(s) 629
FaeI CATG 2 cut(s) 374, 547
FaiI YATR 6 cut(s) 161, 372, 389, 414, 512, 545
FaqI GGGAC 1 cut(s) 525
FatI CATG 2 cut(s) 370, 543
FauI CCCGC 1 cut(s) 447
Fnu4HI GCNGC 8 cut(s) 120, 129, 174, 238, 435, 750, 759, 783
FokI GGATG 1 cut(s) 510
FriOI GRGCYC 1 cut(s) 629
Fsp4HI GCNGC 8 cut(s) 120, 129, 174, 238, 435, 750, 759, 783
FspBI CTAG 1 cut(s) 137
FspI TGCGCA 1 cut(s) 327
GlaI GCGC 4 cut(s) 61, 327, 352, 493
GluI GCNGC 8 cut(s) 120, 129, 174, 238, 435, 750, 759, 783
HaeII RGCGCY 1 cut(s) 354
HaeIII GGCC 2 cut(s) 94, 791
HapII CCGG 1 cut(s) 195
HgaI GACGC 3 cut(s) 61, 140, 608
HhaI GCGC 4 cut(s) 62, 328, 353, 494
Hin1II CATG 2 cut(s) 374, 547
Hin6I GCGC 4 cut(s) 60, 326, 351, 492
HinP1I GCGC 4 cut(s) 60, 326, 351, 492
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HindIII AAGCTT 2 cut(s) 267, 561
HinfI GANTC 4 cut(s) 47, 215, 479, 685
HpaII CCGG 1 cut(s) 195
Hpy166II GTNNAC 2 cut(s) 67, 76
Hpy188I TCNGA 5 cut(s) 106, 559, 724, 800, 817
Hpy188III TCNNGA 6 cut(s) 35, 44, 137, 311, 533, 593
Hpy8I GTNNAC 2 cut(s) 67, 76
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 3 cut(s) 139, 161, 739
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 700
HpyCH4V TGCA 6 cut(s) 88, 262, 317, 370, 712, 758
HpyF10VI GCNNNNNNNGC 7 cut(s) 125, 128, 323, 755, 758, 782, 788
HpyF3I CTNAG 7 cut(s) 205, 339, 475, 531, 628, 786, 828
HpySE526I ACGT 1 cut(s) 700
Hsp92II CATG 2 cut(s) 374, 547
HspAI GCGC 4 cut(s) 60, 326, 351, 492
Kzo9I GATC 2 cut(s) 133, 283
LmnI GCTCC 3 cut(s) 148, 196, 800
Lsp1109I GCAGC 6 cut(s) 106, 160, 421, 761, 769, 770
LweI GCATC 2 cut(s) 304, 763
MaeI CTAG 1 cut(s) 137
MaeII ACGT 1 cut(s) 700
MaeIII GTNAC 1 cut(s) 696
MalI GATC 2 cut(s) 135, 285
MboI GATC 2 cut(s) 133, 283
MboII GAAGA 8 cut(s) 91, 224, 499, 543, 546, 608, 627, 743
MfeI CAATTG 2 cut(s) 304, 753
MflI RGATCY 1 cut(s) 283
MhlI GDGCHC 2 cut(s) 629, 812
MluCI AATT 4 cut(s) 107, 294, 304, 753
MlyI GAGTC 3 cut(s) 56, 224, 473
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 7 cut(s) 14, 31, 176, 242, 540, 697, 781
MseI TTAA 1 cut(s) 669
MslI CAYNNNNRTG 1 cut(s) 369
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 2 cut(s) 195, 254
MunI CAATTG 2 cut(s) 304, 753
MvaI CCWGG 1 cut(s) 254
MwoI GCNNNNNNNGC 7 cut(s) 125, 128, 323, 755, 758, 782, 788
NciI CCSGG 1 cut(s) 195
NdeII GATC 2 cut(s) 133, 283
NlaIII CATG 2 cut(s) 374, 547
NmeAIII GCCGAG 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 696
NsbI TGCGCA 1 cut(s) 327
PfeI GAWTC 1 cut(s) 685
PkrI GCNGC 8 cut(s) 121, 130, 175, 239, 436, 751, 760, 784
PleI GAGTC 3 cut(s) 55, 223, 473
PmaCI CACGTG 1 cut(s) 701
PmlI CACGTG 1 cut(s) 701
PpsI GAGTC 3 cut(s) 55, 223, 473
Ppu21I YACGTR 1 cut(s) 701
Psp124BI GAGCTC 1 cut(s) 629
Psp6I CCWGG 1 cut(s) 252
PspCI CACGTG 1 cut(s) 701
PspGI CCWGG 1 cut(s) 252
PspPI GGNCC 1 cut(s) 92
PstNI CAGNNNCTG 1 cut(s) 323
PsuI RGATCY 1 cut(s) 283
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
RseI CAYNNNNRTG 1 cut(s) 369
SacI GAGCTC 1 cut(s) 629
SaqAI TTAA 1 cut(s) 669
SatI GCNGC 8 cut(s) 120, 129, 174, 238, 435, 750, 759, 783
Sau3AI GATC 2 cut(s) 133, 283
Sau96I GGNCC 1 cut(s) 92
SchI GAGTC 3 cut(s) 56, 224, 473
ScrFI CCNGG 2 cut(s) 195, 254
SduI GDGCHC 2 cut(s) 629, 812
SfaNI GCATC 2 cut(s) 304, 763
SmiMI CAYNNNNRTG 1 cut(s) 369
Sse9I AATT 4 cut(s) 107, 294, 304, 753
SsiI CCGC 3 cut(s) 129, 237, 440
SspMI CTAG 1 cut(s) 137
SstI GAGCTC 1 cut(s) 629
StyD4I CCNGG 2 cut(s) 193, 252
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 1 cut(s) 703
TaqI TCGA 2 cut(s) 111, 452
TasI AATT 4 cut(s) 107, 294, 304, 753
TauI GCSGC 2 cut(s) 131, 240
TfiI GAWTC 1 cut(s) 685
Tru1I TTAA 1 cut(s) 669
Tru9I TTAA 1 cut(s) 669
TscAI CASTG 2 cut(s) 74, 587
TseFI GTSAC 1 cut(s) 696
TseI GCWGC 6 cut(s) 119, 173, 434, 749, 758, 782
Tsp45I GTSAC 1 cut(s) 696
TspDTI ATGAA 1 cut(s) 499
TspGWI ACGGA 2 cut(s) 33, 697
TspRI CASTG 2 cut(s) 74, 587
XapI RAATTY 1 cut(s) 107
XbaI TCTAGA 1 cut(s) 136
XspI CTAG 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.