Rroxscaffold_6G00416100

Ataxin-3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0016089
Physical Location & Seq
Reverse (-)
38163034 .. 38166090
3057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00416100.1

Sequence Viewer

Length: 1206 bp
ATGGAAGGGTCGAGCAACGGAGGGATGCTGTACCACGAGGTACAGGAGTCGAAGCTGTGCGCCGTGCATTGCGTCAACACGGCGCTGCAGGGTCCTTTCTTCTCCGAATTCGATTTGGCCGCCCTCGCCTCCGATCTCGATCGCAAGGAGCGCCAGATGATGCAGCTCAACCTCCAACCCGGACCCAACGCCGCCGCCGCCGCCGCCGGCGATTTCCTCTCCGAGGAGTCCCACAACGTCTCCCTCGACGGCGATTTCAGTATCCAGTTTCATAGATGCTTTAAAGTTGCGGTATCAGAGTTCAGGCTCTCTATGAATGATGCTTTGAGTTCTATTACAAGATTGCTTAGCTCTCTGGTGGAAAAATGTGCTCTCTGTGGGTATTTTCTTAGTTTCCATTCAGTGCATTGCATGTCAGCTGATCAGGTACTACAAAAGGCTTTAGAGGTTTGGGATCTGCAGGTCATCCCCCTTGATTCTCCAGTTGCAGAGCCAGCTCAGATCGATCCTGAGCTGGAAAATGGGTTTATATGTCATTTACATGATCACTGGTTCTGTATCAGGAAAGTGAATGGGGAGTGGTATAATTTTGACAGTCTGTATGCAGCCCCACTTCACCTTTCAAAATTTTACCTCTCGGCCTATTTAGACACGCTGAAGGGCTCTGGTTGGAGCATTTTCCTGTGGCTTTCACCTGAAGATGCCGACAGAATAACTAAATCCTGCAACTCCACAAATGCTCCACGCCAGACTCAGAGAACAAATCCTCGACAACCTTCAGACCAATTCCTATCTACCGAGGAAGCAGAGCTGCTATCAGAGATGGAAGACGAGGACATGAAGGCTGCAATAGCTGCCAGTCTTATGGATTCTTCCCCACCTGTGGCCAATGCCGGAGCTAGCAACGTGGTCAATGCCGGTGCTAGCAATGTGGCCAATGTCGGAGCTAGCAACGTGGTCAATGCCGGAGCTAGCAATGTGGCCAATGTCGGAGCTAGCAACGTGGTCAATGCCGGAGCTAGCAATGTGGCCAATGTCGGAGCTAGCAACGTGGTCAGTGCTGGAGCTAGCAACGTGGTCAGTGCCGGAGCTAGCAATGTGGCCAATGTCGGAGCTAGCAACGTGGCCAATGCTGGAGCTAGCAACCCGCAGAATAGCTCAAACAGCCAAGAGAACAAGATCAGCCAAGAGAATAAAATTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Josephin

Protein Analysis

401

Amino Acids

42.6

Weight (kDa)

4.72

Isoelectric Point (pI)

34.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Josephin PF02099 15 - 90 2.1e-16 Josephin
Josephin PF02099 142 - 229 1.2e-28 Josephin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54130
fragaria_vesca FvH4_6g12170
malus_domestica MD04G1146200.v1.1 MD12G1159400.v1.1
prunus_persica Prupe.6G269800_v2.0.a1
pyrus_communis pycom04g13180
rosa_chinensis RchiOBHm_Chr3g0463971
rosa_laevigata RLG00000024731
rosa_multiflora Rmu_sc0006492.1_g000007
rosa_roxburghii Rroxscaffold_6G00416100
rosa_rugosa Rorug03G0069700
rosa_samantha Rh3AG126600 Rh3BG130800 Rh3DG132000
rosa_wichuraiana Rw3G010690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 451
AciI CCGC 8 cut(s) 120, 192, 195, 198, 201, 204, 290, 1148
AclWI GGATC 2 cut(s) 462, 500
AcoI YGGCCR 7 cut(s) 117, 885, 933, 981, 1029, 1101, 1125
AcsI RAATTY 2 cut(s) 107, 626
AcuI CTGAAG 3 cut(s) 677, 717, 762
AfaI GTAC 3 cut(s) 32, 42, 429
AfiI CCNNNNNNNGG 2 cut(s) 223, 883
AgsI TTSAA 1 cut(s) 624
Alw21I GWGCWC 1 cut(s) 373
Alw26I GTCTC 1 cut(s) 244
AlwI GGATC 2 cut(s) 462, 500
AoxI GGCC 8 cut(s) 117, 639, 885, 933, 981, 1029, 1101, 1125
ApeKI GCWGC 6 cut(s) 85, 163, 605, 811, 845, 854
ApoI RAATTY 2 cut(s) 107, 626
AspLEI GCGC 3 cut(s) 62, 85, 153
AspS9I GGNCC 2 cut(s) 92, 182
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 2 cut(s) 608, 684
AvaII GGWCC 2 cut(s) 92, 182
BaeI ACNNNNGTAYC 2 cut(s) 32, 65
BalI TGGCCA 6 cut(s) 887, 935, 983, 1031, 1103, 1127
BanII GRGCYC 1 cut(s) 665
BauI CACGAG 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 834
Bbv12I GWGCWC 1 cut(s) 373
BbvI GCAGC 6 cut(s) 72, 175, 617, 798, 832, 841
BccI CCATC 1 cut(s) 817
BceAI ACGGC 3 cut(s) 47, 96, 265
BciVI GTATCC 1 cut(s) 272
BclI TGATCA 2 cut(s) 421, 544
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 244
BfmI CTRYAG 2 cut(s) 86, 458
BfoI RGCGCY 2 cut(s) 86, 154
BfuAI ACCTGC 1 cut(s) 451
BfuI GTATCC 1 cut(s) 272
BlpI GCTNAGC 1 cut(s) 347
Bme1390I CCNGG 1 cut(s) 180
Bme18I GGWCC 2 cut(s) 92, 182
BmgT120I GGNCC 2 cut(s) 92, 182
BmiI GGNNCC 2 cut(s) 93, 184
BmrFI CCNGG 1 cut(s) 180
BmsI GCATC 5 cut(s) 15, 150, 266, 310, 691
BpiI GAAGAC 1 cut(s) 834
BpmI CTGGAG 3 cut(s) 465, 1083, 1155
Bpu10I CCTNAGC 1 cut(s) 510
Bpu1102I GCTNAGC 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 180
Bsa29I ATCGAT 1 cut(s) 504
BsaBI GATNNNNATC 1 cut(s) 138
BsaJI CCNNGG 2 cut(s) 222, 798
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 724, 754
Bsc4I CCNNNNNNNGG 2 cut(s) 223, 883
Bse118I RCCGGY 2 cut(s) 206, 917
Bse1I ACTGG 4 cut(s) 265, 482, 554, 858
Bse3DI GCAATG 6 cut(s) 67, 406, 934, 982, 1030, 1102
Bse8I GATNNNNATC 1 cut(s) 138
BseCI ATCGAT 1 cut(s) 504
BseDI CCNNGG 2 cut(s) 222, 798
BseGI GGATG 2 cut(s) 30, 465
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 2 cut(s) 223, 883
BseMI GCAATG 6 cut(s) 67, 406, 934, 982, 1030, 1102
BseMII CTCAG 3 cut(s) 501, 512, 767
BseNI ACTGG 4 cut(s) 265, 482, 554, 858
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 6 cut(s) 72, 175, 617, 798, 832, 841
Bsh1285I CGRYCG 1 cut(s) 142
BshFI GGCC 8 cut(s) 119, 641, 887, 935, 983, 1031, 1103, 1127
BshVI ATCGAT 1 cut(s) 504
BsiEI CGRYCG 1 cut(s) 142
BsiHKAI GWGCWC 1 cut(s) 373
BsiSI CCGG 7 cut(s) 180, 207, 894, 918, 966, 1014, 1086
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 2 cut(s) 223, 883
BsmAI GTCTC 1 cut(s) 244
BsmBI CGTCTC 1 cut(s) 244
BsmFI GGGAC 1 cut(s) 214
BsnI GGCC 8 cut(s) 119, 641, 887, 935, 983, 1031, 1103, 1127
Bsp1286I GDGCHC 2 cut(s) 373, 665
Bsp143I GATC 8 cut(s) 133, 139, 421, 454, 501, 505, 544, 1179
Bsp1720I GCTNAGC 1 cut(s) 347
BspACI CCGC 8 cut(s) 120, 192, 195, 198, 201, 204, 290, 1148
BspANI GGCC 8 cut(s) 119, 641, 887, 935, 983, 1031, 1103, 1127
BspCNI CTCAG 3 cut(s) 502, 511, 766
BspDI ATCGAT 1 cut(s) 504
BspLI GGNNCC 2 cut(s) 93, 184
BspMAI CTGCAG 2 cut(s) 90, 462
BspMI ACCTGC 1 cut(s) 451
BspPI GGATC 2 cut(s) 462, 500
BsrDI GCAATG 6 cut(s) 67, 406, 934, 982, 1030, 1102
BsrFI RCCGGY 2 cut(s) 206, 917
BsrI ACTGG 4 cut(s) 265, 482, 554, 858
BssAI RCCGGY 2 cut(s) 206, 917
BssECI CCNNGG 2 cut(s) 222, 798
BssMI GATC 8 cut(s) 133, 139, 421, 454, 501, 505, 544, 1179
BssSI CACGAG 1 cut(s) 35
Bst2BI CACGAG 1 cut(s) 35
Bst4CI ACNGT 1 cut(s) 596
BstAPI GCANNNNNTGC 1 cut(s) 854
BstDEI CTNAG 5 cut(s) 347, 389, 498, 510, 753
BstENI CCTNNNNNAGG 1 cut(s) 221
BstF5I GGATG 2 cut(s) 30, 465
BstH2I RGCGCY 2 cut(s) 86, 154
BstHHI GCGC 3 cut(s) 62, 85, 153
BstKTI GATC 8 cut(s) 136, 142, 424, 457, 504, 508, 547, 1182
BstMAI GTCTC 1 cut(s) 244
BstMBI GATC 8 cut(s) 133, 139, 421, 454, 501, 505, 544, 1179
BstMCI CGRYCG 1 cut(s) 142
BstMWI GCNNNNNNNGC 9 cut(s) 125, 150, 197, 200, 203, 494, 851, 854, 1164
BstNSI RCATGY 1 cut(s) 415
BstSCI CCNGG 1 cut(s) 178
BstSFI CTRYAG 2 cut(s) 86, 458
BstV1I GCAGC 6 cut(s) 72, 175, 617, 798, 832, 841
BstV2I GAAGAC 1 cut(s) 834
BstX2I RGATCY 1 cut(s) 454
BstXI CCANNNNNNTGG 1 cut(s) 865
BstYI RGATCY 1 cut(s) 454
Bsu15I ATCGAT 1 cut(s) 504
BsuI GTATCC 1 cut(s) 272
BsuRI GGCC 8 cut(s) 119, 641, 887, 935, 983, 1031, 1103, 1127
BsuTUI ATCGAT 1 cut(s) 504
BtsCI GGATG 2 cut(s) 30, 465
BtsIMutI CAGTG 4 cut(s) 408, 547, 1063, 1087
BveI ACCTGC 1 cut(s) 451
CfoI GCGC 3 cut(s) 62, 85, 153
Cfr10I RCCGGY 2 cut(s) 206, 917
Cfr13I GGNCC 2 cut(s) 92, 182
ClaI ATCGAT 1 cut(s) 504
CseI GACGC 1 cut(s) 61
Csp6I GTAC 3 cut(s) 31, 41, 428
CviAII CATG 3 cut(s) 412, 542, 838
CviQI GTAC 3 cut(s) 31, 41, 428
DdeI CTNAG 5 cut(s) 347, 389, 498, 510, 753
DpnI GATC 8 cut(s) 135, 141, 423, 456, 503, 507, 546, 1181
DpnII GATC 8 cut(s) 133, 139, 421, 454, 501, 505, 544, 1179
DraI TTTAAA 1 cut(s) 283
EaeI YGGCCR 7 cut(s) 117, 885, 933, 981, 1029, 1101, 1125
Eco24I GRGCYC 1 cut(s) 665
Eco47I GGWCC 2 cut(s) 92, 182
Eco57I CTGAAG 3 cut(s) 677, 717, 762
EcoNI CCTNNNNNAGG 1 cut(s) 221
EcoO109I RGGNCCY 1 cut(s) 92
EcoRI GAATTC 1 cut(s) 107
EcoT38I GRGCYC 1 cut(s) 665
Esp3I CGTCTC 1 cut(s) 244
FaeI CATG 3 cut(s) 415, 545, 841
FaqI GGGAC 1 cut(s) 214
FatI CATG 3 cut(s) 411, 541, 837
FauI CCCGC 1 cut(s) 1155
FbaI TGATCA 2 cut(s) 421, 544
FokI GGATG 2 cut(s) 37, 452
FriOI GRGCYC 1 cut(s) 665
GlaI GCGC 3 cut(s) 61, 84, 152
GsuI CTGGAG 3 cut(s) 465, 1083, 1155
HaeII RGCGCY 2 cut(s) 86, 154
HaeIII GGCC 8 cut(s) 119, 641, 887, 935, 983, 1031, 1103, 1127
HapII CCGG 7 cut(s) 180, 207, 894, 918, 966, 1014, 1086
HgaI GACGC 1 cut(s) 61
HhaI GCGC 3 cut(s) 62, 85, 153
Hin1II CATG 3 cut(s) 415, 545, 841
Hin6I GCGC 3 cut(s) 60, 83, 151
HinP1I GCGC 3 cut(s) 60, 83, 151
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 5 cut(s) 47, 227, 476, 751, 869
HpaII CCGG 7 cut(s) 180, 207, 894, 918, 966, 1014, 1086
HphI GGTGA 2 cut(s) 608, 684
Hpy166II GTNNAC 1 cut(s) 76
Hpy188III TCNNGA 3 cut(s) 137, 509, 562
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 3 cut(s) 652, 786, 835
HpyCH4III ACNGT 1 cut(s) 596
HpyCH4IV ACGT 7 cut(s) 237, 906, 954, 1002, 1050, 1074, 1122
HpyF10VI GCNNNNNNNGC 9 cut(s) 125, 150, 197, 200, 203, 494, 851, 854, 1164
HpyF3I CTNAG 5 cut(s) 347, 389, 498, 510, 753
HpySE526I ACGT 7 cut(s) 237, 906, 954, 1002, 1050, 1074, 1122
Hsp92II CATG 3 cut(s) 415, 545, 841
HspAI GCGC 3 cut(s) 60, 83, 151
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Ksp22I TGATCA 2 cut(s) 421, 544
Kzo9I GATC 8 cut(s) 133, 139, 421, 454, 501, 505, 544, 1179
Lsp1109I GCAGC 6 cut(s) 72, 175, 617, 798, 832, 841
LweI GCATC 5 cut(s) 15, 150, 266, 310, 691
MaeII ACGT 7 cut(s) 237, 906, 954, 1002, 1050, 1074, 1122
MalI GATC 8 cut(s) 135, 141, 423, 456, 503, 507, 546, 1181
MboI GATC 8 cut(s) 133, 139, 421, 454, 501, 505, 544, 1179
MboII GAAGA 4 cut(s) 91, 710, 839, 864
MflI RGATCY 1 cut(s) 454
MhlI GDGCHC 2 cut(s) 373, 665
MlsI TGGCCA 6 cut(s) 887, 935, 983, 1031, 1103, 1127
MluCI AATT 5 cut(s) 107, 586, 626, 785, 1197
MluNI TGGCCA 6 cut(s) 887, 935, 983, 1031, 1103, 1127
MlyI GAGTC 3 cut(s) 56, 236, 745
MmeI TCCRAC 6 cut(s) 199, 650, 922, 970, 1018, 1090
Mox20I TGGCCA 6 cut(s) 887, 935, 983, 1031, 1103, 1127
MreI CGCCGGCG 1 cut(s) 206
MroNI GCCGGC 1 cut(s) 206
MscI TGGCCA 6 cut(s) 887, 935, 983, 1031, 1103, 1127
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 1 cut(s) 540
Msp20I TGGCCA 6 cut(s) 887, 935, 983, 1031, 1103, 1127
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 7 cut(s) 180, 207, 894, 918, 966, 1014, 1086
MspR9I CCNGG 1 cut(s) 180
MwoI GCNNNNNNNGC 9 cut(s) 125, 150, 197, 200, 203, 494, 851, 854, 1164
NaeI GCCGGC 1 cut(s) 208
NciI CCSGG 1 cut(s) 180
NdeII GATC 8 cut(s) 133, 139, 421, 454, 501, 505, 544, 1179
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 3 cut(s) 415, 545, 841
NlaIV GGNNCC 2 cut(s) 93, 184
NmeAIII GCCGAG 1 cut(s) 617
NspI RCATGY 1 cut(s) 415
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 2 cut(s) 476, 869
Ple19I CGATCG 1 cut(s) 142
PleI GAGTC 3 cut(s) 55, 235, 745
PpsI GAGTC 3 cut(s) 55, 235, 745
PpuMI RGGWCCY 1 cut(s) 92
Psp5II RGGWCCY 1 cut(s) 92
PspN4I GGNNCC 2 cut(s) 93, 184
PspPI GGNCC 2 cut(s) 92, 182
PspPPI RGGWCCY 1 cut(s) 92
PstI CTGCAG 2 cut(s) 90, 462
PsuI RGATCY 1 cut(s) 454
PvuI CGATCG 1 cut(s) 142
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 3 cut(s) 32, 42, 429
RsaNI GTAC 3 cut(s) 31, 41, 428
RseI CAYNNNNRTG 1 cut(s) 540
SaqAI TTAA 1 cut(s) 282
Sau3AI GATC 8 cut(s) 133, 139, 421, 454, 501, 505, 544, 1179
Sau96I GGNCC 2 cut(s) 92, 182
SchI GAGTC 3 cut(s) 56, 236, 745
ScrFI CCNGG 1 cut(s) 180
SduI GDGCHC 2 cut(s) 373, 665
SfaNI GCATC 5 cut(s) 15, 150, 266, 310, 691
SfcI CTRYAG 2 cut(s) 86, 458
SgrAI CRCCGGYG 1 cut(s) 206
SinI GGWCC 2 cut(s) 92, 182
SmiMI CAYNNNNRTG 1 cut(s) 540
Sse9I AATT 5 cut(s) 107, 586, 626, 785, 1197
SsiI CCGC 8 cut(s) 120, 192, 195, 198, 201, 204, 290, 1148
StyD4I CCNGG 1 cut(s) 178
TaaI ACNGT 1 cut(s) 596
TaiI ACGT 7 cut(s) 240, 909, 957, 1005, 1053, 1077, 1125
TaqI TCGA 7 cut(s) 11, 50, 111, 138, 246, 504, 769
TasI AATT 5 cut(s) 107, 586, 626, 785, 1197
TauI GCSGC 6 cut(s) 122, 194, 197, 200, 203, 206
TfiI GAWTC 2 cut(s) 476, 869
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 4 cut(s) 408, 554, 1063, 1087
TseI GCWGC 6 cut(s) 85, 163, 605, 811, 845, 854
TspDTI ATGAA 3 cut(s) 260, 329, 854
TspGWI ACGGA 1 cut(s) 33
TspRI CASTG 4 cut(s) 408, 554, 1063, 1087
VpaK11BI GGWCC 2 cut(s) 92, 182
XagI CCTNNNNNAGG 1 cut(s) 221
XapI RAATTY 2 cut(s) 107, 626
XceI RCATGY 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.