pycom04g13180

Ataxin-3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0016089
Physical Location & Seq
Reverse (-)
16177892 .. 16181013
3122 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g13180.1

Sequence Viewer

Length: 1209 bp
ATGGAAGGGGCGAGCAACGGAGGGATGTTGTACCACGAGGTACAAGAGTCGAAGCTCTGCGCCGTGCATTGCGTGAACACGGTGCTGCAGGGTCCCTTCTTCTCCGAATTCGATTTGGCGGCGCTCGCCTCCGATCTCGATCGGAAGGAGCGGCAGATGATGCTCGTCGAGGGCGGCTCACACGCCGGCGATTTCCTCTCCGAGGAGTCTCATAACGTCTCCTTGGACGGCGATTTCAGTATTCAGGTTCTACAAAAGGCTTTAGAGGTTTGGGATCTGCAGATCATCCCCCTTGATTCTCCGGTTGCTGAGCCCGCCCAGATAGATCCTGAGCTTGAAAATGCATTTATCTGTCATTTGCAGGATCATTGGTTTTGTATCCGGAAAGTGAGTGGAGAGTGGTATAATTTTGACAGTCTTTATGCGGCTCCATTGCATCTCTCAAAGTTTTACCTATCAGCCTATCTGGACACACTAAAAGGCTCGGGTTGGAGCATTTTCCTGGTGAGAGGCAACTTCCCAAAAGAGTGTCCCATCTCATCCTCCGAAGCTTCTAATGGTTATGGGCAGTGGCTTTCACCTGAAGATGCTGAGAGGATAACTAAATCCTGCAACTCCACAAATGCCCAACCTGAAAGAACAAATTTGAATCCACAATCTTCCGCACAATTTCTATCAAATGAGGAAGCAGATTTGCTTTCAGAAATGGAAGACGAGGACTTGAAAGCTGCTATAGCTGCCAGTCTTATGGATTCTACCCCAGCCATTGCCTGTGCTCAAGCCGGAAGCCCACATTATAGCAGCAGAAACCCTCAGGACAGCAACGACAACCCTCAGAACAGCACCCAAAACCCTCAAAACAGCACCGACAACTCTCAGAACACCACCCAAAACCCTCAGGACAGCAACGACAACCCTCAGAACAGCACCCAAAACTCTCAAGACAGCACCGACAACTCTCAGAACAGCACCCAAAACCCTCAAGACAGCACCGACAACTCTCAGAACATTACCGGAAACCCCCAGAAAATTACCGGAAACCCTCAGAACATTACCGGAACCTCTCAGAACATTACCGGAACCCCTCAGAACATTACTGGAAATCCTCAGAACATTACTGGCAGCCCTCAGATCAGCACTGGCAATCCTCGGATCAGCACTAGCAACCCTCAGATCGAAAGTTCAGAGAGCCTTGAGAAAATTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Josephin

Protein Analysis

403

Amino Acids

43.84

Weight (kDa)

4.28

Isoelectric Point (pI)

53.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Josephin PF02099 15 - 170 9.4e-54 Josephin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54130
fragaria_vesca FvH4_6g12170
malus_domestica MD04G1146200.v1.1 MD12G1159400.v1.1
prunus_persica Prupe.6G269800_v2.0.a1
pyrus_communis pycom04g13180
rosa_chinensis RchiOBHm_Chr3g0463971
rosa_laevigata RLG00000024731
rosa_multiflora Rmu_sc0006492.1_g000007
rosa_roxburghii Rroxscaffold_6G00416100
rosa_rugosa Rorug03G0069700
rosa_samantha Rh3AG126600 Rh3BG130800 Rh3DG132000
rosa_wichuraiana Rw3G010690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 151
AccIII TCCGGA 1 cut(s) 381
AciI CCGC 6 cut(s) 119, 151, 174, 315, 425, 663
AclWI GGATC 4 cut(s) 282, 320, 372, 1160
AcsI RAATTY 2 cut(s) 107, 643
AcuI CTGAAG 1 cut(s) 603
AfaI GTAC 2 cut(s) 32, 42
AfiI CCNNNNNNNGG 1 cut(s) 202
AgsI TTSAA 3 cut(s) 338, 649, 724
AjnI CCWGG 1 cut(s) 501
AluBI AGCT 5 cut(s) 55, 334, 551, 728, 737
AluI AGCT 5 cut(s) 55, 334, 551, 728, 737
Alw21I GWGCWC 1 cut(s) 778
Alw26I GTCTC 2 cut(s) 213, 223
AlwI GGATC 4 cut(s) 282, 320, 372, 1160
Ama87I CYCGRG 1 cut(s) 484
Aor13HI TCCGGA 1 cut(s) 381
ApeKI GCWGC 5 cut(s) 85, 728, 737, 801, 1122
ApoI RAATTY 2 cut(s) 107, 643
AspLEI GCGC 2 cut(s) 62, 124
AspS9I GGNCC 1 cut(s) 92
AsuHPI GGTGA 2 cut(s) 517, 570
AvaI CYCGRG 1 cut(s) 484
AvaII GGWCC 1 cut(s) 92
AxyI CCTNAGG 2 cut(s) 813, 897
BaeI ACNNNNGTAYC 2 cut(s) 32, 65
BanII GRGCYC 1 cut(s) 315
BauI CACGAG 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 717
Bbv12I GWGCWC 1 cut(s) 778
BbvI GCAGC 5 cut(s) 72, 715, 724, 813, 1134
BccI CCATC 1 cut(s) 542
BceAI ACGGC 2 cut(s) 47, 244
BciT130I CCWGG 1 cut(s) 503
BciVI GTATCC 1 cut(s) 389
BcoDI GTCTC 2 cut(s) 213, 223
BfaI CTAG 1 cut(s) 1161
BfmI CTRYAG 3 cut(s) 86, 278, 732
BfoI RGCGCY 1 cut(s) 125
BfuI GTATCC 1 cut(s) 389
BisI GCNGC 9 cut(s) 86, 120, 152, 175, 426, 729, 738, 802, 1123
BlpI GCTNAGC 1 cut(s) 309
BlsI GCNGC 9 cut(s) 87, 121, 153, 176, 427, 730, 739, 803, 1124
Bme1390I CCNGG 1 cut(s) 503
Bme18I GGWCC 1 cut(s) 92
BmeT110I CYCGRG 1 cut(s) 484
BmgT120I GGNCC 1 cut(s) 92
BmiI GGNNCC 5 cut(s) 93, 94, 429, 1060, 1081
BmrFI CCNGG 1 cut(s) 503
BmsI GCATC 3 cut(s) 150, 445, 577
BpiI GAAGAC 1 cut(s) 717
BplI GAGNNNNNCTC 2 cut(s) 161, 193
Bpu10I CCTNAGC 1 cut(s) 330
Bpu1102I GCTNAGC 1 cut(s) 309
BpuEI CTTGAG 3 cut(s) 762, 924, 966
BsaBI GATNNNNATC 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 201, 222, 1148
BsaWI WCCGGW 6 cut(s) 301, 381, 1013, 1034, 1055, 1076
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 4 cut(s) 741, 1102, 1123, 1144
Bse21I CCTNAGG 2 cut(s) 813, 897
Bse3DI GCAATG 3 cut(s) 67, 431, 765
Bse8I GATNNNNATC 1 cut(s) 138
BseAI TCCGGA 1 cut(s) 381
BseBI CCWGG 1 cut(s) 503
BseDI CCNNGG 3 cut(s) 201, 222, 1148
BseGI GGATG 3 cut(s) 30, 285, 539
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 1 cut(s) 202
BseMI GCAATG 3 cut(s) 67, 431, 765
BseNI ACTGG 4 cut(s) 741, 1102, 1123, 1144
BseRI GAGGAG 1 cut(s) 218
BseXI GCAGC 5 cut(s) 72, 715, 724, 813, 1134
BseYI CCCAGC 1 cut(s) 760
Bsh1285I CGRYCG 1 cut(s) 142
BsiEI CGRYCG 1 cut(s) 142
BsiHKAI GWGCWC 1 cut(s) 778
BsiHKCI CYCGRG 1 cut(s) 484
BsiSI CCGG 8 cut(s) 186, 302, 382, 783, 1014, 1035, 1056, 1077
BslFI GGGAC 2 cut(s) 78, 516
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 2 cut(s) 213, 223
BsmBI CGTCTC 1 cut(s) 223
BsmFI GGGAC 2 cut(s) 78, 516
BsoBI CYCGRG 1 cut(s) 484
Bsp1286I GDGCHC 2 cut(s) 315, 778
Bsp13I TCCGGA 1 cut(s) 381
Bsp143I GATC 9 cut(s) 133, 139, 274, 282, 325, 364, 1131, 1152, 1173
Bsp1720I GCTNAGC 1 cut(s) 309
BspACI CCGC 6 cut(s) 119, 151, 174, 315, 425, 663
BspEI TCCGGA 1 cut(s) 381
BspLI GGNNCC 5 cut(s) 93, 94, 429, 1060, 1081
BspMAI CTGCAG 2 cut(s) 90, 282
BspPI GGATC 4 cut(s) 282, 320, 372, 1160
BsrBI CCGCTC 1 cut(s) 151
BsrDI GCAATG 3 cut(s) 67, 431, 765
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 4 cut(s) 741, 1102, 1123, 1144
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 3 cut(s) 201, 222, 1148
BssMI GATC 9 cut(s) 133, 139, 274, 282, 325, 364, 1131, 1152, 1173
BssSI CACGAG 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 222
Bst2BI CACGAG 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 503
Bst4CI ACNGT 2 cut(s) 82, 416
BstAPI GCANNNNNTGC 1 cut(s) 160
BstC8I GCNNGC 4 cut(s) 13, 126, 187, 315
BstENI CCTNNNNNAGG 1 cut(s) 200
BstF5I GGATG 3 cut(s) 30, 285, 539
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 2 cut(s) 62, 124
BstKTI GATC 9 cut(s) 136, 142, 277, 285, 328, 367, 1134, 1155, 1176
BstMAI GTCTC 2 cut(s) 213, 223
BstMBI GATC 9 cut(s) 133, 139, 274, 282, 325, 364, 1131, 1152, 1173
BstMCI CGRYCG 1 cut(s) 142
BstMWI GCNNNNNNNGC 5 cut(s) 125, 160, 314, 734, 737
BstNI CCWGG 1 cut(s) 503
BstSCI CCNGG 1 cut(s) 501
BstSFI CTRYAG 3 cut(s) 86, 278, 732
BstV1I GCAGC 5 cut(s) 72, 715, 724, 813, 1134
BstV2I GAAGAC 1 cut(s) 717
BstX2I RGATCY 2 cut(s) 274, 325
BstXI CCANNNNNNTGG 1 cut(s) 748
BstYI RGATCY 2 cut(s) 274, 325
Bsu36I CCTNAGG 2 cut(s) 813, 897
BsuI GTATCC 1 cut(s) 389
BtsCI GGATG 3 cut(s) 30, 285, 539
BtsI GCAGTG 1 cut(s) 575
BtsIMutI CAGTG 2 cut(s) 575, 1137
Cac8I GCNNGC 4 cut(s) 13, 126, 187, 315
CfoI GCGC 2 cut(s) 62, 124
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 1 cut(s) 92
Csp6I GTAC 2 cut(s) 31, 41
CviQI GTAC 2 cut(s) 31, 41
DpnI GATC 9 cut(s) 135, 141, 276, 284, 327, 366, 1133, 1154, 1175
DpnII GATC 9 cut(s) 133, 139, 274, 282, 325, 364, 1131, 1152, 1173
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 315
Eco47I GGWCC 1 cut(s) 92
Eco57I CTGAAG 1 cut(s) 603
Eco81I CCTNAGG 2 cut(s) 813, 897
Eco88I CYCGRG 1 cut(s) 484
EcoNI CCTNNNNNAGG 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 92
EcoRI GAATTC 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 501
EcoT14I CCWWGG 1 cut(s) 222
EcoT22I ATGCAT 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 222
Esp3I CGTCTC 1 cut(s) 223
FaiI YATR 7 cut(s) 213, 405, 423, 564, 734, 749, 798
FaqI GGGAC 2 cut(s) 78, 516
FauI CCCGC 1 cut(s) 322
Fnu4HI GCNGC 9 cut(s) 86, 120, 152, 175, 426, 729, 738, 802, 1123
FokI GGATG 3 cut(s) 37, 272, 526
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 9 cut(s) 86, 120, 152, 175, 426, 729, 738, 802, 1123
FspBI CTAG 1 cut(s) 1161
GlaI GCGC 2 cut(s) 61, 123
GluI GCNGC 9 cut(s) 86, 120, 152, 175, 426, 729, 738, 802, 1123
GsaI CCCAGC 1 cut(s) 764
HaeII RGCGCY 1 cut(s) 125
HapII CCGG 8 cut(s) 186, 302, 382, 783, 1014, 1035, 1056, 1077
HhaI GCGC 2 cut(s) 62, 124
Hin6I GCGC 2 cut(s) 60, 122
HinP1I GCGC 2 cut(s) 60, 122
HindIII AAGCTT 1 cut(s) 549
HinfI GANTC 5 cut(s) 47, 206, 296, 649, 752
HpaII CCGG 8 cut(s) 186, 302, 382, 783, 1014, 1035, 1056, 1077
HphI GGTGA 2 cut(s) 517, 570
Hpy166II GTNNAC 1 cut(s) 76
Hpy188III TCNNGA 8 cut(s) 137, 329, 382, 467, 815, 899, 941, 983
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 2 cut(s) 106, 139
HpyCH4III ACNGT 2 cut(s) 82, 416
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 7 cut(s) 67, 88, 280, 344, 361, 436, 612
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 160, 314, 734, 737
HpySE526I ACGT 1 cut(s) 216
HspAI GCGC 2 cut(s) 60, 122
KflI GGGWCCC 1 cut(s) 92
Kpn2I TCCGGA 1 cut(s) 381
KroI GCCGGC 1 cut(s) 185
KroNI GCCGGC 1 cut(s) 187
Kzo9I GATC 9 cut(s) 133, 139, 274, 282, 325, 364, 1131, 1152, 1173
LmnI GCTCC 3 cut(s) 148, 433, 492
Lsp1109I GCAGC 5 cut(s) 72, 715, 724, 813, 1134
LweI GCATC 3 cut(s) 150, 445, 577
MaeI CTAG 1 cut(s) 1161
MaeII ACGT 1 cut(s) 216
MalI GATC 9 cut(s) 135, 141, 276, 284, 327, 366, 1133, 1154, 1175
MbiI CCGCTC 1 cut(s) 151
MboI GATC 9 cut(s) 133, 139, 274, 282, 325, 364, 1131, 1152, 1173
MboII GAAGA 4 cut(s) 91, 596, 651, 722
MflI RGATCY 2 cut(s) 274, 325
MhlI GDGCHC 2 cut(s) 315, 778
MluCI AATT 6 cut(s) 107, 406, 643, 668, 1029, 1200
MlyI GAGTC 2 cut(s) 56, 215
MmeI TCCRAC 1 cut(s) 470
Mph1103I ATGCAT 1 cut(s) 346
MreI CGCCGGCG 1 cut(s) 185
MroI TCCGGA 1 cut(s) 381
MroNI GCCGGC 1 cut(s) 185
MspI CCGG 8 cut(s) 186, 302, 382, 783, 1014, 1035, 1056, 1077
MspR9I CCNGG 1 cut(s) 503
MvaI CCWGG 1 cut(s) 503
MwoI GCNNNNNNNGC 5 cut(s) 125, 160, 314, 734, 737
NaeI GCCGGC 1 cut(s) 187
NdeII GATC 9 cut(s) 133, 139, 274, 282, 325, 364, 1131, 1152, 1173
NgoMIV GCCGGC 1 cut(s) 185
NlaIV GGNNCC 5 cut(s) 93, 94, 429, 1060, 1081
NsiI ATGCAT 1 cut(s) 346
PdiI GCCGGC 1 cut(s) 187
PfeI GAWTC 3 cut(s) 296, 649, 752
PkrI GCNGC 9 cut(s) 87, 121, 153, 176, 427, 730, 739, 803, 1124
Ple19I CGATCG 1 cut(s) 142
PleI GAGTC 2 cut(s) 55, 214
PpsI GAGTC 2 cut(s) 55, 214
PpuMI RGGWCCY 1 cut(s) 92
Psp5II RGGWCCY 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 501
PspFI CCCAGC 1 cut(s) 760
PspGI CCWGG 1 cut(s) 501
PspN4I GGNNCC 5 cut(s) 93, 94, 429, 1060, 1081
PspPI GGNCC 1 cut(s) 92
PspPPI RGGWCCY 1 cut(s) 92
PstI CTGCAG 2 cut(s) 90, 282
PsuI RGATCY 2 cut(s) 274, 325
PvuI CGATCG 1 cut(s) 142
RsaI GTAC 2 cut(s) 32, 42
RsaNI GTAC 2 cut(s) 31, 41
SatI GCNGC 9 cut(s) 86, 120, 152, 175, 426, 729, 738, 802, 1123
Sau3AI GATC 9 cut(s) 133, 139, 274, 282, 325, 364, 1131, 1152, 1173
Sau96I GGNCC 1 cut(s) 92
SchI GAGTC 2 cut(s) 56, 215
ScrFI CCNGG 1 cut(s) 503
SduI GDGCHC 2 cut(s) 315, 778
SfaNI GCATC 3 cut(s) 150, 445, 577
SfcI CTRYAG 3 cut(s) 86, 278, 732
SgrAI CRCCGGYG 1 cut(s) 185
SinI GGWCC 1 cut(s) 92
SmlI CTYRAG 4 cut(s) 777, 939, 981, 1193
SmoI CTYRAG 4 cut(s) 777, 939, 981, 1193
Sse9I AATT 6 cut(s) 107, 406, 643, 668, 1029, 1200
SsiI CCGC 6 cut(s) 119, 151, 174, 315, 425, 663
SspMI CTAG 1 cut(s) 1161
StyD4I CCNGG 1 cut(s) 501
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 2 cut(s) 82, 416
TaiI ACGT 1 cut(s) 219
TaqI TCGA 5 cut(s) 50, 111, 138, 168, 1176
TasI AATT 6 cut(s) 107, 406, 643, 668, 1029, 1200
TauI GCSGC 4 cut(s) 122, 154, 177, 428
TfiI GAWTC 3 cut(s) 296, 649, 752
TscAI CASTG 2 cut(s) 575, 1144
TseI GCWGC 5 cut(s) 85, 728, 737, 801, 1122
TspGWI ACGGA 1 cut(s) 33
TspRI CASTG 2 cut(s) 575, 1144
VpaK11BI GGWCC 1 cut(s) 92
XagI CCTNNNNNAGG 1 cut(s) 200
XapI RAATTY 2 cut(s) 107, 643
XspI CTAG 1 cut(s) 1161
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.