MD12G1159400.v1.1

Ataxin-3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016089
Physical Location & Seq
Reverse (-)
23940451 .. 23943769
3319 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1159400.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGGAAGGGGCGAGCAACGGAGGGATGTTGTACCACGAGGTACAAGAGTCGAAGCTCTGCGCGGTGCATTGCGTGAACACGGTGCTGCAGGGTCCCTTCTTCTCTGAATTCGATTTGGCGGCGCTCGCCTCCGATCTCGATCGGAAGGAGCGGCAGATGATGCTCGTCGAGGGCGGCTTCCACGCCGGTGATTTCCTCTCCGAGGAGTCACATAACGTCTCCTTGGACGGCGATTTCAGTATTCAGGTCCTACAAAAGGCTTTAGAGGTTTGGGATCTGCAGGTCATCCCCCTTGATTCTCCTGTCGCTGAGCCAGCCCAGATTGATCCTGAGCTTGAAAATGCATTTATCTGTCATTTGCAGGACCATTGGTTTTGTATCCGGAAAGTGAGTAGAGAGTGGTATAATTTCGACAGTCTTTATGCGGCACCATTGCACCTCTCAAAGTTTTACCTTTCGGCCTATCTGGACACACTAAAAGGCTCTGGTTGGAGCATTTTCCTGGTGAGAGGAAACTTCCCAAAAGAGTGTCCCATCTCATCCTCTGAAGCTTCTAATGGTTACGGGCAGTGGCTTTCACCTGAAGATGCTGAGAGGATAACTAAATCCTGCAACTCCACAAATGCTGAAGCTCAGGGAACAGATTCAAATCCACAATCTTCGGCACAAGTTCTATCAAATGAGGAAGCAGATTTGCTTTCAGAAATGGAAGATGAGGACTTGAAAGCTGCTATAGCTGCCAGTCTTTTGGATTCTACTCCATCCATTGCCCGTGCTCAAGCCAGAAACCCACAGATCAGCAGCAGAAACCCTCAGGACAGCATCGACAACCCTCAGAACAACACCAGAAACCTTCAAGGCATCACTGACAATCCTCAGAACAGCATTGGAAACCCTCAGGACAGCAACGACAACCCTCAGAACAACACCAGAAACCTTAAAGACATCACTGACAATCCTCAGAACAGCATCGGAAACCCTCAGGACAGCAACGACAACCTTCAGCACAACACCGACCTTCAAGACAGCACTGACAATCCTCAGAACAGCATCGAAAACCCTCAGAACATTAGCGGCAACCCTCAGATCAGTACTGGCAACCCTCAGAACGAAAGTGCAGGCAGCCAAGAGAAAATTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Josephin

Protein Analysis

381

Amino Acids

41.81

Weight (kDa)

4.3

Isoelectric Point (pI)

50.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Josephin PF02099 15 - 170 4.2e-53 Josephin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54130
fragaria_vesca FvH4_6g12170
malus_domestica MD04G1146200.v1.1 MD12G1159400.v1.1
prunus_persica Prupe.6G269800_v2.0.a1
pyrus_communis pycom04g13180
rosa_chinensis RchiOBHm_Chr3g0463971
rosa_laevigata RLG00000024731
rosa_multiflora Rmu_sc0006492.1_g000007
rosa_roxburghii Rroxscaffold_6G00416100
rosa_rugosa Rorug03G0069700
rosa_samantha Rh3AG126600 Rh3BG130800 Rh3DG132000
rosa_wichuraiana Rw3G010690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 271
AccB1I GGYRCC 1 cut(s) 427
AccBSI CCGCTC 1 cut(s) 151
AccII CGCG 1 cut(s) 62
AccIII TCCGGA 1 cut(s) 381
AciI CCGC 6 cut(s) 62, 119, 151, 174, 425, 1074
AclWI GGATC 2 cut(s) 282, 320
AcsI RAATTY 1 cut(s) 107
AcuI CTGAAG 4 cut(s) 567, 603, 648, 986
AfaI GTAC 3 cut(s) 32, 42, 1093
AfiI CCNNNNNNNGG 2 cut(s) 202, 256
AgsI TTSAA 5 cut(s) 338, 648, 724, 857, 1022
AjnI CCWGG 1 cut(s) 501
AleI CACNNNNGTG 1 cut(s) 186
AluBI AGCT 6 cut(s) 55, 334, 551, 632, 728, 737
AluI AGCT 6 cut(s) 55, 334, 551, 632, 728, 737
Alw21I GWGCWC 1 cut(s) 778
Alw26I GTCTC 1 cut(s) 223
AlwI GGATC 2 cut(s) 282, 320
Aor13HI TCCGGA 1 cut(s) 381
AoxI GGCC 1 cut(s) 459
ApeKI GCWGC 5 cut(s) 85, 728, 737, 801, 1122
ApoI RAATTY 1 cut(s) 107
Asp700I GAANNNNTTC 1 cut(s) 643
AspLEI GCGC 2 cut(s) 62, 124
AspS9I GGNCC 3 cut(s) 92, 247, 364
AsuHPI GGTGA 3 cut(s) 200, 517, 570
AvaII GGWCC 3 cut(s) 92, 247, 364
AxyI CCTNAGG 3 cut(s) 813, 897, 981
BaeI ACNNNNGTAYC 2 cut(s) 32, 65
BanI GGYRCC 1 cut(s) 427
BauI CACGAG 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 778
BbvI GCAGC 5 cut(s) 72, 715, 724, 813, 1134
BccI CCATC 2 cut(s) 542, 769
BceAI ACGGC 1 cut(s) 244
BciT130I CCWGG 1 cut(s) 503
BciVI GTATCC 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 223
BfmI CTRYAG 3 cut(s) 86, 278, 732
BfoI RGCGCY 1 cut(s) 125
BfuAI ACCTGC 1 cut(s) 271
BfuI GTATCC 1 cut(s) 389
BlpI GCTNAGC 1 cut(s) 309
BmcAI AGTACT 1 cut(s) 1093
Bme1390I CCNGG 1 cut(s) 503
Bme18I GGWCC 3 cut(s) 92, 247, 364
BmgT120I GGNCC 3 cut(s) 92, 247, 364
BmiI GGNNCC 3 cut(s) 93, 94, 429
BmrFI CCNGG 1 cut(s) 503
BmsI GCATC 6 cut(s) 150, 577, 831, 870, 978, 1059
Bpu10I CCTNAGC 2 cut(s) 330, 633
Bpu1102I GCTNAGC 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 762
BsaBI GATNNNNATC 2 cut(s) 138, 648
BsaJI CCNNGG 2 cut(s) 201, 222
BsaWI WCCGGW 1 cut(s) 381
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 256
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 741, 1099
Bse21I CCTNAGG 3 cut(s) 813, 897, 981
Bse3DI GCAATG 3 cut(s) 67, 431, 765
Bse8I GATNNNNATC 2 cut(s) 138, 648
BseAI TCCGGA 1 cut(s) 381
BseBI CCWGG 1 cut(s) 503
BseDI CCNNGG 2 cut(s) 201, 222
BseGI GGATG 4 cut(s) 30, 285, 539, 761
BseJI GATNNNNATC 2 cut(s) 138, 648
BseLI CCNNNNNNNGG 2 cut(s) 202, 256
BseMI GCAATG 3 cut(s) 67, 431, 765
BseNI ACTGG 2 cut(s) 741, 1099
BseRI GAGGAG 1 cut(s) 218
BseXI GCAGC 5 cut(s) 72, 715, 724, 813, 1134
BsgI GTGCAG 1 cut(s) 1137
Bsh1236I CGCG 1 cut(s) 62
Bsh1285I CGRYCG 1 cut(s) 142
BshFI GGCC 1 cut(s) 461
BshNI GGYRCC 1 cut(s) 427
BsiEI CGRYCG 1 cut(s) 142
BsiHKAI GWGCWC 1 cut(s) 778
BsiSI CCGG 2 cut(s) 186, 382
BslFI GGGAC 2 cut(s) 78, 516
BslI CCNNNNNNNGG 2 cut(s) 202, 256
BsmAI GTCTC 1 cut(s) 223
BsmBI CGTCTC 1 cut(s) 223
BsmFI GGGAC 2 cut(s) 78, 516
BsnI GGCC 1 cut(s) 461
Bsp1286I GDGCHC 1 cut(s) 778
Bsp13I TCCGGA 1 cut(s) 381
Bsp143I GATC 6 cut(s) 133, 139, 274, 325, 795, 1086
Bsp1720I GCTNAGC 1 cut(s) 309
BspACI CCGC 6 cut(s) 62, 119, 151, 174, 425, 1074
BspANI GGCC 1 cut(s) 461
BspEI TCCGGA 1 cut(s) 381
BspFNI CGCG 1 cut(s) 62
BspLI GGNNCC 3 cut(s) 93, 94, 429
BspMAI CTGCAG 2 cut(s) 90, 282
BspMI ACCTGC 1 cut(s) 271
BspPI GGATC 2 cut(s) 282, 320
BspT107I GGYRCC 1 cut(s) 427
BsrBI CCGCTC 1 cut(s) 151
BsrDI GCAATG 3 cut(s) 67, 431, 765
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 2 cut(s) 741, 1099
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 2 cut(s) 201, 222
BssMI GATC 6 cut(s) 133, 139, 274, 325, 795, 1086
BssSI CACGAG 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 222
Bst2BI CACGAG 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 503
Bst4CI ACNGT 2 cut(s) 82, 416
BstAPI GCANNNNNTGC 1 cut(s) 160
BstC8I GCNNGC 4 cut(s) 13, 126, 315, 1120
BstENI CCTNNNNNAGG 2 cut(s) 200, 254
BstF5I GGATG 4 cut(s) 30, 285, 539, 761
BstFNI CGCG 1 cut(s) 62
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 2 cut(s) 62, 124
BstKTI GATC 6 cut(s) 136, 142, 277, 328, 798, 1089
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 6 cut(s) 133, 139, 274, 325, 795, 1086
BstMCI CGRYCG 1 cut(s) 142
BstMWI GCNNNNNNNGC 5 cut(s) 125, 160, 314, 734, 737
BstNI CCWGG 1 cut(s) 503
BstSCI CCNGG 1 cut(s) 501
BstSFI CTRYAG 3 cut(s) 86, 278, 732
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 5 cut(s) 72, 715, 724, 813, 1134
BstX2I RGATCY 1 cut(s) 274
BstXI CCANNNNNNTGG 1 cut(s) 748
BstYI RGATCY 1 cut(s) 274
Bsu36I CCTNAGG 3 cut(s) 813, 897, 981
BsuI GTATCC 1 cut(s) 389
BsuRI GGCC 1 cut(s) 461
BtsCI GGATG 4 cut(s) 30, 285, 539, 761
BtsI GCAGTG 1 cut(s) 575
BtsIMutI CAGTG 4 cut(s) 575, 864, 948, 1029
BveI ACCTGC 1 cut(s) 271
Cac8I GCNNGC 4 cut(s) 13, 126, 315, 1120
CfoI GCGC 2 cut(s) 62, 124
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 3 cut(s) 92, 247, 364
Csp6I GTAC 3 cut(s) 31, 41, 1092
CviQI GTAC 3 cut(s) 31, 41, 1092
DpnI GATC 6 cut(s) 135, 141, 276, 327, 797, 1088
DpnII GATC 6 cut(s) 133, 139, 274, 325, 795, 1086
Eco130I CCWWGG 1 cut(s) 222
Eco47I GGWCC 3 cut(s) 92, 247, 364
Eco57I CTGAAG 4 cut(s) 567, 603, 648, 986
Eco81I CCTNAGG 3 cut(s) 813, 897, 981
EcoNI CCTNNNNNAGG 2 cut(s) 200, 254
EcoO109I RGGNCCY 2 cut(s) 92, 247
EcoRI GAATTC 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 501
EcoT14I CCWWGG 1 cut(s) 222
EcoT22I ATGCAT 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 222
Esp3I CGTCTC 1 cut(s) 223
FaiI YATR 4 cut(s) 213, 405, 423, 734
FaqI GGGAC 2 cut(s) 78, 516
FokI GGATG 4 cut(s) 37, 272, 526, 748
GlaI GCGC 2 cut(s) 61, 123
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 1 cut(s) 461
HapII CCGG 2 cut(s) 186, 382
HhaI GCGC 2 cut(s) 62, 124
Hin6I GCGC 2 cut(s) 60, 122
HinP1I GCGC 2 cut(s) 60, 122
HindIII AAGCTT 1 cut(s) 549
HinfI GANTC 5 cut(s) 47, 206, 296, 644, 752
HpaII CCGG 2 cut(s) 186, 382
HphI GGTGA 3 cut(s) 200, 517, 570
Hpy166II GTNNAC 1 cut(s) 76
Hpy188III TCNNGA 8 cut(s) 137, 329, 382, 467, 815, 899, 983, 1022
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 5 cut(s) 106, 139, 863, 1010, 1028
HpyCH4III ACNGT 2 cut(s) 82, 416
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 8 cut(s) 67, 88, 280, 344, 361, 436, 612, 1118
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 160, 314, 734, 737
HpySE526I ACGT 1 cut(s) 216
HspAI GCGC 2 cut(s) 60, 122
KflI GGGWCCC 1 cut(s) 92
Kpn2I TCCGGA 1 cut(s) 381
Kzo9I GATC 6 cut(s) 133, 139, 274, 325, 795, 1086
LmnI GCTCC 2 cut(s) 148, 492
Lsp1109I GCAGC 5 cut(s) 72, 715, 724, 813, 1134
LweI GCATC 6 cut(s) 150, 577, 831, 870, 978, 1059
MaeII ACGT 1 cut(s) 216
MaeIII GTNAC 2 cut(s) 207, 560
MalI GATC 6 cut(s) 135, 141, 276, 327, 797, 1088
MbiI CCGCTC 1 cut(s) 151
MboI GATC 6 cut(s) 133, 139, 274, 325, 795, 1086
MboII GAAGA 4 cut(s) 91, 596, 651, 722
MflI RGATCY 1 cut(s) 274
MhlI GDGCHC 1 cut(s) 778
MluCI AATT 3 cut(s) 107, 406, 1134
MlyI GAGTC 2 cut(s) 56, 215
MmeI TCCRAC 1 cut(s) 470
Mph1103I ATGCAT 1 cut(s) 346
MroI TCCGGA 1 cut(s) 381
MroXI GAANNNNTTC 1 cut(s) 643
MseI TTAA 1 cut(s) 939
MslI CAYNNNNRTG 1 cut(s) 186
MspI CCGG 2 cut(s) 186, 382
MspR9I CCNGG 1 cut(s) 503
MvaI CCWGG 1 cut(s) 503
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 5 cut(s) 125, 160, 314, 734, 737
NdeII GATC 6 cut(s) 133, 139, 274, 325, 795, 1086
NlaIV GGNNCC 3 cut(s) 93, 94, 429
NmuCI GTSAC 1 cut(s) 207
NsiI ATGCAT 1 cut(s) 346
OliI CACNNNNGTG 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 643
PfeI GAWTC 3 cut(s) 296, 644, 752
Ple19I CGATCG 1 cut(s) 142
PleI GAGTC 2 cut(s) 55, 214
PpsI GAGTC 2 cut(s) 55, 214
PpuMI RGGWCCY 2 cut(s) 92, 247
Psp5II RGGWCCY 2 cut(s) 92, 247
Psp6I CCWGG 1 cut(s) 501
PspGI CCWGG 1 cut(s) 501
PspN4I GGNNCC 3 cut(s) 93, 94, 429
PspPI GGNCC 3 cut(s) 92, 247, 364
PspPPI RGGWCCY 2 cut(s) 92, 247
PstI CTGCAG 2 cut(s) 90, 282
PsuI RGATCY 1 cut(s) 274
PvuI CGATCG 1 cut(s) 142
RsaI GTAC 3 cut(s) 32, 42, 1093
RsaNI GTAC 3 cut(s) 31, 41, 1092
RseI CAYNNNNRTG 1 cut(s) 186
SaqAI TTAA 1 cut(s) 939
Sau3AI GATC 6 cut(s) 133, 139, 274, 325, 795, 1086
Sau96I GGNCC 3 cut(s) 92, 247, 364
ScaI AGTACT 1 cut(s) 1093
SchI GAGTC 2 cut(s) 56, 215
ScrFI CCNGG 1 cut(s) 503
SduI GDGCHC 1 cut(s) 778
SfaNI GCATC 6 cut(s) 150, 577, 831, 870, 978, 1059
SfcI CTRYAG 3 cut(s) 86, 278, 732
SgrAI CRCCGGYG 1 cut(s) 185
SinI GGWCC 3 cut(s) 92, 247, 364
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 1 cut(s) 777
SmoI CTYRAG 1 cut(s) 777
Sse9I AATT 3 cut(s) 107, 406, 1134
SsiI CCGC 6 cut(s) 62, 119, 151, 174, 425, 1074
StyD4I CCNGG 1 cut(s) 501
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 2 cut(s) 82, 416
TaiI ACGT 1 cut(s) 219
TaqI TCGA 7 cut(s) 50, 111, 138, 168, 411, 825, 1053
TasI AATT 3 cut(s) 107, 406, 1134
TatI WGTACW 1 cut(s) 1091
TauI GCSGC 5 cut(s) 122, 154, 177, 428, 1077
TfiI GAWTC 3 cut(s) 296, 644, 752
Tru1I TTAA 1 cut(s) 939
Tru9I TTAA 1 cut(s) 939
TscAI CASTG 4 cut(s) 575, 871, 955, 1036
TseFI GTSAC 1 cut(s) 207
TseI GCWGC 5 cut(s) 85, 728, 737, 801, 1122
Tsp45I GTSAC 1 cut(s) 207
TspGWI ACGGA 1 cut(s) 33
TspRI CASTG 4 cut(s) 575, 871, 955, 1036
VpaK11BI GGWCC 3 cut(s) 92, 247, 364
XagI CCTNNNNNAGG 2 cut(s) 200, 254
XapI RAATTY 1 cut(s) 107
XmnI GAANNNNTTC 1 cut(s) 643
ZrmI AGTACT 1 cut(s) 1093
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.