AT3G54510

Cytosolic domain of 10TM putative phosphate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005105
Physical Location & Seq
Forward (+)
20180007 .. 20183588
3582 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G54510.2

Sequence Viewer

Length: 1854 bp
ATGTGCAGAATTAGATTCTTCCTGATGTGTTCGTTACTTGGAGCATCACTACTTCTTCCGGTTGATTATTACAATGAGTCAGATTTACCGACACGAAGAGAGTATTCAATGGATGCTTTCACAATATCAAATATCACACGAGGTTCTAACAAGTTATGGGTGCATTTCTCATGCTTGTGGTGCATATCATTCTATGCATTGTTTTTGTTGCATAAGGAATATAAGGAGATTCTTGTGATAAGGCTTCAACAAATGAAAGAACTTAGACATCGTGCTGATCAGTTCACTGTTCTTGTCCGCCAAGTTCCTCTCTGCCCTGAGCATAACACTCGTGGCTGCGCCGTTGATCACTTTTTCTCTAAGCATCATCGCTTTAGTTATCATTCACATCAGATGTTGTATGACGGGAGAGATCTTGAATACCTTTTGGGGAAGCAGAAGAAGCTTAAGAAAGAGCTAGAAGACAAGAGACACACAGAAATACTTTCAAATGGATCACAAGAACACAAGCAAATATCCACATCTGAAGAAAAACTTCGAGAAATTACTCATATGATTTACCACCTTCAAAGTGAAACTATGCTCAGAGAGAAGGAGTTACCTGTTGCGTTTGTCACCTTCAAATCCCGAAGAAATGCCGCGTTGGCAGCTCAAACACAGCAGCACTCAAATCCTCTAGAACTGATAACTGAAATGGCTCCTGAACCAAGAGATGTATCCTGGAGAAATCTTGCTATTCCACAAAAGATTTTGCCTCTCAACAAGATCGGAGTCATCCTTGCAGCAGCACTTCTTACAATCTTCTTTGCTATTCCGGTCACAGCTGTCCAGGGAATTGCAAAGTATGAGAAGCTTAAGAAATGGTTTCCTCCAGCCATGGCTATTGAATTCATACCAGGGCTTAGCTCAGTTGTGACAGGTTACCTCCCAAGTGCTATACTCAAAGGTTTCATGTACATTATTCCTTTCGCTATGCTTGGACTGGCCTATCTCGGCGGCTCTATTTCCAACAGTAAAGAGGAGATAAAAGCTTGCAACATGGTCTTCTATTTTCTAATGGGAAATGTTTTCTTCTTGAGTCTTATCTCTGGTTCCTTGCTTGATGAAATAGGAGAATATCTCACTCATCCTAGAGACATTCCTAGTCACCTTGCTGCTGCTGTTTCAGCCCAGGCAGAGTTTTTTATGACATACATCTTGACAGATGGGCTGTCTGGATTTTCTTTGGAGATTCTTCAATTGGGACTTATACTATTTGATATCATAAGATCATACACTTATGGAAGAGGAAAAGAGAGAACTCCTTACCTCTTTTCATTTCCATATTTTAGAGTTATCCCCACAGTTTCCTTATCGATAATGATCGGTATGATTTATGCGGTGGTTGCGCCATTGATGCTCCCTTTCCTAGTTGGTTATTTCTGCCTTGGATACATTGTCTACTTCAATCAGATGGAGGATGTTTACGAGACTACATATGATACTTGCGGTCGATTCTGGCCATTCATTCATCATTATATATTTGTCTCAATAATACTAATGCAAATCACAATGGTGGGTCTTTTTGGACTTAAATCAAAGCCATCTGCAGCAATTGCTACAGTACCTCTCATATTGATCACTATAGCTTATAACGAATACTGCAAGATCCGCTTCCTCCCATCATTTAAACATTTCCCTATACAGACAGCAGTTGAAATAGACGAAGAAGATGAAAAGAATGGGGAGATGGAAACACATTATGTTGATGCTGCAACGGCGTATAACCGGCATCAACCTTGCTTAGAGCGTGTAAGCTCAGCGGAATCACCAACAAACTTAAGCCAACCATTGCTTGGGACAGACTCCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

617

Amino Acids

70.65

Weight (kDa)

7.06

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 6 - 168 3.9e-38 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 189 - 338 8.6e-23 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 350 - 617 8e-77 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1632
AccB7I CCANNNNNTGG 1 cut(s) 1835
AccI GTMKAC 1 cut(s) 1440
AccII CGCG 1 cut(s) 641
AciI CCGC 7 cut(s) 298, 639, 996, 1379, 1488, 1651, 1802
AclWI GGATC 2 cut(s) 502, 1642
AcoI YGGCCR 1 cut(s) 1499
AcsI RAATTY 1 cut(s) 887
AcuI CTGAAG 1 cut(s) 546
AfaI GTAC 2 cut(s) 956, 1605
AfiI CCNNNNNNNGG 1 cut(s) 1835
AflII CTTAAG 3 cut(s) 446, 854, 1819
AjnI CCWGG 4 cut(s) 719, 828, 895, 1170
AleI CACNNNNGTG 1 cut(s) 1553
AluBI AGCT 9 cut(s) 445, 457, 650, 824, 853, 906, 1031, 1628, 1797
AluI AGCT 9 cut(s) 445, 457, 650, 824, 853, 906, 1031, 1628, 1797
Alw26I GTCTC 4 cut(s) 463, 1128, 1463, 1531
AlwI GGATC 2 cut(s) 502, 1642
AoxI GGCC 2 cut(s) 984, 1499
ApeKI GCWGC 9 cut(s) 336, 647, 661, 782, 785, 1154, 1157, 1589, 1751
ApoI RAATTY 1 cut(s) 887
Asp700I GAANNNNTTC 1 cut(s) 534
AspLEI GCGC 2 cut(s) 341, 1390
AsuHPI GGTGA 3 cut(s) 607, 1139, 1800
BalI TGGCCA 1 cut(s) 1501
BauI CACGAG 2 cut(s) 138, 330
BbsI GAAGAC 2 cut(s) 468, 1036
BbvI GCAGC 9 cut(s) 323, 659, 673, 794, 797, 1141, 1144, 1601, 1738
BccI CCATC 5 cut(s) 1199, 1447, 1591, 1669, 1723
BceAI ACGGC 2 cut(s) 326, 1773
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 4 cut(s) 721, 830, 897, 1172
BciVI GTATCC 2 cut(s) 727, 1424
BclI TGATCA 3 cut(s) 277, 346, 1617
BcoDI GTCTC 4 cut(s) 463, 1128, 1463, 1531
BfaI CTAG 5 cut(s) 458, 677, 1131, 1143, 1409
BfmI CTRYAG 3 cut(s) 1587, 1599, 1623
BfrI CTTAAG 3 cut(s) 446, 854, 1819
BfuI GTATCC 2 cut(s) 727, 1424
BglI GCCNNNNNGGC 1 cut(s) 644
BglII AGATCT 1 cut(s) 412
BlpI GCTNAGC 2 cut(s) 902, 1798
Bme1390I CCNGG 4 cut(s) 721, 830, 897, 1172
BmiI GGNNCC 2 cut(s) 699, 1093
BmrFI CCNGG 4 cut(s) 721, 830, 897, 1172
BmsI GCATC 6 cut(s) 53, 103, 373, 1386, 1738, 1780
BpiI GAAGAC 2 cut(s) 468, 1036
BplI GAGNNNNNCTC 2 cut(s) 1104, 1136
BpmI CTGGAG 2 cut(s) 742, 855
Bpu10I CCTNAGC 1 cut(s) 318
Bpu1102I GCTNAGC 2 cut(s) 902, 1798
BpuEI CTTGAG 1 cut(s) 1096
Bsa29I ATCGAT 1 cut(s) 1355
BsaBI GATNNNNATC 1 cut(s) 1361
BsaJI CCNNGG 5 cut(s) 829, 876, 896, 1170, 1426
BsaWI WCCGGW 2 cut(s) 58, 814
Bsc4I CCNNNNNNNGG 1 cut(s) 1835
Bse118I RCCGGY 1 cut(s) 1767
Bse1I ACTGG 1 cut(s) 987
Bse3DI GCAATG 1 cut(s) 1829
Bse8I GATNNNNATC 1 cut(s) 1361
BseBI CCWGG 4 cut(s) 721, 830, 897, 1172
BseCI ATCGAT 1 cut(s) 1355
BseDI CCNNGG 5 cut(s) 829, 876, 896, 1170, 1426
BseGI GGATG 4 cut(s) 118, 774, 1126, 1465
BseJI GATNNNNATC 1 cut(s) 1361
BseLI CCNNNNNNNGG 1 cut(s) 1835
BseMI GCAATG 1 cut(s) 1829
BseMII CTCAG 4 cut(s) 309, 598, 921, 1812
BseNI ACTGG 1 cut(s) 987
BseRI GAGGAG 1 cut(s) 1034
BseXI GCAGC 9 cut(s) 323, 659, 673, 794, 797, 1141, 1144, 1601, 1738
BsgI GTGCAG 1 cut(s) 25
Bsh1236I CGCG 1 cut(s) 641
Bsh1285I CGRYCG 1 cut(s) 1492
BshFI GGCC 2 cut(s) 986, 1501
BshVI ATCGAT 1 cut(s) 1355
BsiEI CGRYCG 1 cut(s) 1492
BsiSI CCGG 3 cut(s) 59, 815, 1768
BslFI GGGAC 1 cut(s) 1257
BslI CCNNNNNNNGG 1 cut(s) 1835
BsmAI GTCTC 4 cut(s) 463, 1128, 1463, 1531
BsmFI GGGAC 1 cut(s) 1257
BsnI GGCC 2 cut(s) 986, 1501
Bsp1407I TGTACA 1 cut(s) 954
Bsp143I GATC 9 cut(s) 277, 346, 412, 494, 765, 1268, 1362, 1617, 1647
Bsp1720I GCTNAGC 2 cut(s) 902, 1798
Bsp19I CCATGG 1 cut(s) 876
BspACI CCGC 7 cut(s) 298, 639, 996, 1379, 1488, 1651, 1802
BspANI GGCC 2 cut(s) 986, 1501
BspCNI CTCAG 4 cut(s) 310, 597, 920, 1811
BspDI ATCGAT 1 cut(s) 1355
BspFNI CGCG 1 cut(s) 641
BspLI GGNNCC 2 cut(s) 699, 1093
BspMAI CTGCAG 1 cut(s) 1591
BspPI GGATC 2 cut(s) 502, 1642
BspTI CTTAAG 3 cut(s) 446, 854, 1819
BsrDI GCAATG 1 cut(s) 1829
BsrFI RCCGGY 1 cut(s) 1767
BsrGI TGTACA 1 cut(s) 954
BsrI ACTGG 1 cut(s) 987
BssAI RCCGGY 1 cut(s) 1767
BssECI CCNNGG 5 cut(s) 829, 876, 896, 1170, 1426
BssMI GATC 9 cut(s) 277, 346, 412, 494, 765, 1268, 1362, 1617, 1647
BssSI CACGAG 2 cut(s) 138, 330
BssT1I CCWWGG 2 cut(s) 876, 1426
Bst2BI CACGAG 2 cut(s) 138, 330
Bst2UI CCWGG 4 cut(s) 721, 830, 897, 1172
Bst4CI ACNGT 4 cut(s) 289, 1013, 1345, 1603
Bst6I CTCTTC 2 cut(s) 91, 1279
BstAFI CTTAAG 3 cut(s) 446, 854, 1819
BstAPI GCANNNNNTGC 1 cut(s) 1595
BstAUI TGTACA 1 cut(s) 954
BstC8I GCNNGC 1 cut(s) 1033
BstDEI CTNAG 8 cut(s) 263, 318, 360, 584, 902, 907, 1783, 1798
BstDSI CCRYGG 1 cut(s) 876
BstEII GGTNACC 1 cut(s) 920
BstF5I GGATG 4 cut(s) 118, 774, 1126, 1465
BstFNI CGCG 1 cut(s) 641
BstHHI GCGC 2 cut(s) 341, 1390
BstKTI GATC 9 cut(s) 280, 349, 415, 497, 768, 1271, 1365, 1620, 1650
BstMAI GTCTC 4 cut(s) 463, 1128, 1463, 1531
BstMBI GATC 9 cut(s) 277, 346, 412, 494, 765, 1268, 1362, 1617, 1647
BstMCI CGRYCG 1 cut(s) 1492
BstNI CCWGG 4 cut(s) 721, 830, 897, 1172
BstPI GGTNACC 1 cut(s) 920
BstSCI CCNGG 4 cut(s) 719, 828, 895, 1170
BstSFI CTRYAG 3 cut(s) 1587, 1599, 1623
BstUI CGCG 1 cut(s) 641
BstV1I GCAGC 9 cut(s) 323, 659, 673, 794, 797, 1141, 1144, 1601, 1738
BstV2I GAAGAC 2 cut(s) 468, 1036
BstX2I RGATCY 2 cut(s) 412, 1647
BstYI RGATCY 2 cut(s) 412, 1647
Bsu15I ATCGAT 1 cut(s) 1355
BsuI GTATCC 2 cut(s) 727, 1424
BsuRI GGCC 2 cut(s) 986, 1501
BsuTUI ATCGAT 1 cut(s) 1355
BtgI CCRYGG 1 cut(s) 876
BtgZI GCGATG 1 cut(s) 353
BtsCI GGATG 4 cut(s) 118, 774, 1126, 1465
BtsIMutI CAGTG 1 cut(s) 285
Cac8I GCNNGC 1 cut(s) 1033
CfoI GCGC 2 cut(s) 341, 1390
Cfr10I RCCGGY 1 cut(s) 1767
ClaI ATCGAT 1 cut(s) 1355
Csp6I GTAC 2 cut(s) 955, 1604
CviAII CATG 4 cut(s) 171, 877, 952, 1039
CviQI GTAC 2 cut(s) 955, 1604
DdeI CTNAG 8 cut(s) 263, 318, 360, 584, 902, 907, 1783, 1798
DpnI GATC 9 cut(s) 279, 348, 414, 496, 767, 1270, 1364, 1619, 1649
DpnII GATC 9 cut(s) 277, 346, 412, 494, 765, 1268, 1362, 1617, 1647
DraI TTTAAA 1 cut(s) 1669
EaeI YGGCCR 1 cut(s) 1499
Eam1104I CTCTTC 2 cut(s) 91, 1279
EarI CTCTTC 2 cut(s) 91, 1279
EciI GGCGGA 1 cut(s) 287
Eco130I CCWWGG 2 cut(s) 876, 1426
Eco32I GATATC 1 cut(s) 1261
Eco57I CTGAAG 1 cut(s) 546
Eco91I GGTNACC 1 cut(s) 920
EcoO65I GGTNACC 1 cut(s) 920
EcoRI GAATTC 1 cut(s) 887
EcoRII CCWGG 4 cut(s) 719, 828, 895, 1170
EcoRV GATATC 1 cut(s) 1261
EcoT14I CCWWGG 2 cut(s) 876, 1426
EcoT22I ATGCAT 1 cut(s) 199
ErhI CCWWGG 2 cut(s) 876, 1426
FaeI CATG 4 cut(s) 174, 880, 955, 1042
FalI AAGNNNNNCTT 4 cut(s) 519, 551, 1637, 1669
FaqI GGGAC 1 cut(s) 1257
FatI CATG 4 cut(s) 170, 876, 951, 1038
FauNDI CATATG 2 cut(s) 552, 1477
FbaI TGATCA 3 cut(s) 277, 346, 1617
FblI GTMKAC 1 cut(s) 1440
FokI GGATG 4 cut(s) 125, 761, 1113, 1472
FspBI CTAG 5 cut(s) 458, 677, 1131, 1143, 1409
GlaI GCGC 2 cut(s) 340, 1389
GsuI CTGGAG 2 cut(s) 742, 855
HaeIII GGCC 2 cut(s) 986, 1501
HapII CCGG 3 cut(s) 59, 815, 1768
HhaI GCGC 2 cut(s) 341, 1390
Hin1II CATG 4 cut(s) 174, 880, 955, 1042
Hin6I GCGC 2 cut(s) 339, 1388
HinP1I GCGC 2 cut(s) 339, 1388
HindIII AAGCTT 3 cut(s) 443, 851, 1029
HinfI GANTC 9 cut(s) 15, 77, 229, 771, 1078, 1231, 1494, 1805, 1844
HpaII CCGG 3 cut(s) 59, 815, 1768
HphI GGTGA 3 cut(s) 607, 1139, 1800
Hpy166II GTNNAC 3 cut(s) 285, 1441, 1465
Hpy188I TCNGA 6 cut(s) 82, 393, 526, 587, 770, 1452
Hpy188III TCNNGA 9 cut(s) 22, 416, 539, 627, 677, 701, 1075, 1198, 1215
Hpy8I GTNNAC 3 cut(s) 285, 1441, 1465
HpyAV CCTTC 3 cut(s) 575, 586, 628
HpyCH4III ACNGT 4 cut(s) 289, 1013, 1345, 1603
HpyF3I CTNAG 8 cut(s) 263, 318, 360, 584, 902, 907, 1783, 1798
Hsp92II CATG 4 cut(s) 174, 880, 955, 1042
HspAI GCGC 2 cut(s) 339, 1388
Ksp22I TGATCA 3 cut(s) 277, 346, 1617
Kzo9I GATC 9 cut(s) 277, 346, 412, 494, 765, 1268, 1362, 1617, 1647
LmnI GCTCC 3 cut(s) 41, 703, 1404
Lsp1109I GCAGC 9 cut(s) 323, 659, 673, 794, 797, 1141, 1144, 1601, 1738
LweI GCATC 6 cut(s) 53, 103, 373, 1386, 1738, 1780
MaeI CTAG 5 cut(s) 458, 677, 1131, 1143, 1409
MaeIII GTNAC 7 cut(s) 33, 597, 613, 817, 913, 920, 1145
MalI GATC 9 cut(s) 279, 348, 414, 496, 767, 1270, 1364, 1619, 1649
MboI GATC 9 cut(s) 277, 346, 412, 494, 765, 1268, 1362, 1617, 1647
MfeI CAATTG 2 cut(s) 1238, 1593
MflI RGATCY 2 cut(s) 412, 1647
MlsI TGGCCA 1 cut(s) 1501
MluCI AATT 6 cut(s) 9, 543, 834, 887, 1238, 1593
MluNI TGGCCA 1 cut(s) 1501
MlyI GAGTC 4 cut(s) 86, 780, 1087, 1838
MmeI TCCRAC 1 cut(s) 1032
Mox20I TGGCCA 1 cut(s) 1501
Mph1103I ATGCAT 1 cut(s) 199
MroXI GAANNNNTTC 1 cut(s) 534
MscI TGGCCA 1 cut(s) 1501
MseI TTAA 5 cut(s) 447, 855, 1572, 1668, 1820
MslI CAYNNNNRTG 2 cut(s) 175, 1553
Msp20I TGGCCA 1 cut(s) 1501
MspA1I CMGCKG 2 cut(s) 824, 1802
MspCI CTTAAG 3 cut(s) 446, 854, 1819
MspI CCGG 3 cut(s) 59, 815, 1768
MspR9I CCNGG 4 cut(s) 721, 830, 897, 1172
MunI CAATTG 2 cut(s) 1238, 1593
MvaI CCWGG 4 cut(s) 721, 830, 897, 1172
MvnI CGCG 1 cut(s) 641
NcoI CCATGG 1 cut(s) 876
NdeI CATATG 2 cut(s) 552, 1477
NdeII GATC 9 cut(s) 277, 346, 412, 494, 765, 1268, 1362, 1617, 1647
NlaIII CATG 4 cut(s) 174, 880, 955, 1042
NlaIV GGNNCC 2 cut(s) 699, 1093
NmeAIII GCCGAG 1 cut(s) 972
NmuCI GTSAC 4 cut(s) 613, 817, 913, 1145
NsiI ATGCAT 1 cut(s) 199
OliI CACNNNNGTG 1 cut(s) 1553
PdmI GAANNNNTTC 1 cut(s) 534
PfeI GAWTC 5 cut(s) 15, 229, 1231, 1494, 1805
PflMI CCANNNNNTGG 1 cut(s) 1835
PfoI TCCNGGA 1 cut(s) 719
PleI GAGTC 4 cut(s) 85, 779, 1086, 1838
PpsI GAGTC 4 cut(s) 85, 779, 1086, 1838
PsiI TTATAA 1 cut(s) 1632
Psp6I CCWGG 4 cut(s) 719, 828, 895, 1170
PspEI GGTNACC 1 cut(s) 920
PspGI CCWGG 4 cut(s) 719, 828, 895, 1170
PspN4I GGNNCC 2 cut(s) 699, 1093
PstI CTGCAG 1 cut(s) 1591
PsuI RGATCY 2 cut(s) 412, 1647
PvuII CAGCTG 1 cut(s) 824
RsaI GTAC 2 cut(s) 956, 1605
RsaNI GTAC 2 cut(s) 955, 1604
RseI CAYNNNNRTG 2 cut(s) 175, 1553
SaqAI TTAA 5 cut(s) 447, 855, 1572, 1668, 1820
Sau3AI GATC 9 cut(s) 277, 346, 412, 494, 765, 1268, 1362, 1617, 1647
SchI GAGTC 4 cut(s) 86, 780, 1087, 1838
ScrFI CCNGG 4 cut(s) 721, 830, 897, 1172
SfaNI GCATC 6 cut(s) 53, 103, 373, 1386, 1738, 1780
SfcI CTRYAG 3 cut(s) 1587, 1599, 1623
SmiMI CAYNNNNRTG 2 cut(s) 175, 1553
SmlI CTYRAG 4 cut(s) 446, 854, 1075, 1819
SmoI CTYRAG 4 cut(s) 446, 854, 1075, 1819
Sse9I AATT 6 cut(s) 9, 543, 834, 887, 1238, 1593
SsiI CCGC 7 cut(s) 298, 639, 996, 1379, 1488, 1651, 1802
SspMI CTAG 5 cut(s) 458, 677, 1131, 1143, 1409
StyD4I CCNGG 4 cut(s) 719, 828, 895, 1170
StyI CCWWGG 2 cut(s) 876, 1426
TaaI ACNGT 4 cut(s) 289, 1013, 1345, 1603
TaqI TCGA 3 cut(s) 538, 1355, 1492
TasI AATT 6 cut(s) 9, 543, 834, 887, 1238, 1593
TatI WGTACW 1 cut(s) 954
TauI GCSGC 2 cut(s) 641, 999
TfiI GAWTC 5 cut(s) 15, 229, 1231, 1494, 1805
Tru1I TTAA 5 cut(s) 447, 855, 1572, 1668, 1820
Tru9I TTAA 5 cut(s) 447, 855, 1572, 1668, 1820
TscAI CASTG 1 cut(s) 292
TseFI GTSAC 4 cut(s) 613, 817, 913, 1145
TseI GCWGC 9 cut(s) 336, 647, 661, 782, 785, 1154, 1157, 1589, 1751
Tsp45I GTSAC 4 cut(s) 613, 817, 913, 1145
TspDTI ATGAA 8 cut(s) 269, 880, 940, 1119, 1305, 1495, 1499, 1728
TspRI CASTG 1 cut(s) 292
Van91I CCANNNNNTGG 1 cut(s) 1835
Vha464I CTTAAG 3 cut(s) 446, 854, 1819
XapI RAATTY 1 cut(s) 887
XbaI TCTAGA 1 cut(s) 676
XcmI CCANNNNNNNNNTGG 1 cut(s) 1832
XmiI GTMKAC 1 cut(s) 1440
XmnI GAANNNNTTC 1 cut(s) 534
XspI CTAG 5 cut(s) 458, 677, 1131, 1143, 1409
Zsp2I ATGCAT 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.