FvH4_7g02530

Calcium-dependent channel, 7TM region, putative phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005105
Physical Location & Seq
Forward (+)
2810369 .. 2818386
8018 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g02530.t1

Sequence Viewer

Length: 2202 bp
ATGGACCCTGGAAGCCTGATAGCTTCTGCGGGCATAAACATAGGCCTGGCCATCGTGATCCTCTCACTCTTCTCCATCCTCAAGAAGCAACCCTCGTACGCCCCCGTCTACTACCCTCGCCGCCTCTCTCAGTCGCAGTCGTCGCAGCGCAACCGCAATAACGTTCCCAGTTTCAATGGATCCCTCACTCCCCGCCGCTTCCTCCCTTCCCTCTCCTGGATTAGCCGCGCTTACCGCGTCTCCGAGGATGAGATCCTTGAGACCGGTGGCCTCGACGCCCTCATCATCATCAGGCTCTTCAAGTTTGGTATCAAATTCTTTTTTGTATGCTCTCTTATTGGATTGGCAGTACTTCTACCGGTAAACTACAGTGGCCAGGGTCAGCTTGCTATGAGCCGCCAACGGAGTTATCAAGCTCTGAGCCACCATTCCTTGGATCTCTTCAGTATATCTAACATTGAACCAGGTTCTAATAGGCTTTGGGTTCATTTTTCATGCTTATGGTTTATATCTCTGTATGGAGTATATCTACTGTACAAGGAATATCATGAAATTTTGTCTAAAAGGATTCAACAGCTTCGTAATATTAGGCATCAGCCTGATCAGTTTACTGTTCTAGTCCGGGAAATTCCCTTCTGCAACGAACACAGATCTCGCGGATGTTGTGTTGATAAATTTTTCTCGAAGCATCATCCATATGCTTATCATTCCTATCAGATTTTGTATGATGGAAAAGAATTTCAGGCTTTGCTGGGCCAGGCAAAATCCATAGAAAGAAAAATAGAGGACTTGAGCAAGCGGTCCACAGGAAAGAAACATAATGAGATGCCTTTGCTCTCTAATCCTTCTCACCATGATGCTGCCAAATTAGCTTCACATGAGGAAAAGCTTGAAGAACTTCATCACAAGATTCACCAGTTACAGTCTGAAAATAAGCTTAAAGAAAAGGAGTTACCTGTTGCTTTTGTTACATTCAAGACTCGGTTGGGTGCAGCACTAGTTTCCCATTCTCAGCAGCATGCAGACCCACTTCTTTGGATAACTGACATGGCTCCAGAACCAAGGGATGTATCTTGGAGAAATCTGGTGATACCATACAGACTTTTGCCACTTTGCAGGCTAGGTGTCATTGTTGCAGCGACTCTTCTTACAATTTTTTTCGCTATCCCAGTCACTGCTGTTCAGGGAATAGCCAAATTTGAGAAACTAAAAAAATGGTTTCCACCAGCCAAGGCTTTACAGTTGATACCAGGGTTAAGCTCTATCATAACAGGATATCTTCCAAGTGCTGTTCTGAAGGGGTTCATATACATAGTTCCTTTTGCAATGCTGGGAATGGCTAAACTAGCTGGCTGTATATCCAAAAGCAAGGAGGAAATAAAAGCTTCCAACATGGTTTTTTATTTTCTGGTCGGAAATGTGTTCTTTTGGAGCTTGTTATCTGGGTCTTTACTAGATGAAATTGGGGAATCTTTTACTCATCCAAAAGATATTCCTAGTCACCTTGCTACTGCTGTTTCTGCTCAAGCAGACTTCTTTGTGACATATATTTTGACAGATGGACTGTCAGGGTTTTCTTTGGAGATCCTTCAACCAGGTTTACTTTGTTGGGATGCTATAAAGTCTTGTACATATGCCAGAGGGAAAGAGAAAACTCCTTTTCTCTATTCATTACCTTACTACCGAATCATTCCTACTGTCTCACTGTCTGTGCTCATAGGCATGGTATATGCAGTTGTCACACCACTTCTGCTCCCATTTCTAATTGGCTACTTCTGTCTGGGCTATGTTGTCTATATAAACCAGATTGAAGATGTGTATGAAACAGTTTATGACACATGCGGACAGTATTGGCCTTACATCCACCATTATATTCTCATTGCAATCGTTCTTATGCAAATTACTATGATTGGTCTTTTCGGTCTGAAATCAAAGCCAGCTGCATCCATTTCCACTGTTCCACTTCTCTTTCTTACATTGATGTTTAATGAGTATTGCAAGATTCGTTTTCTTCCTACATTCCACCTTTTATCTATCCAGAATGCCAAGGAATTTGATGAACTTGATGTCAAGTGCAATCAGATGGAGTTTAACTATGAGAGTGCAAGCATTGCTTATTGCCCACCTTGTTTGCGTCCCTTGAACGTGATGAAATCAGAGCCGAGCTATTCACCTTCTATTGTTTCTACATCATCGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

734

Amino Acids

82.97

Weight (kDa)

8.93

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 6 - 181 1.3e-39 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 202 - 361 1.8e-23 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 374 - 640 6.7e-76 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 433
AccI GTMKAC 1 cut(s) 108
AccII CGCG 3 cut(s) 228, 237, 657
AclI AACGTT 1 cut(s) 162
AclWI GGATC 6 cut(s) 52, 174, 187, 247, 444, 1579
AcoI YGGCCR 2 cut(s) 48, 373
AcsI RAATTY 7 cut(s) 314, 552, 627, 674, 737, 1196, 2051
AcuI CTGAAG 2 cut(s) 427, 1316
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 4 cut(s) 98, 351, 536, 1630
AfiI CCNNNNNNNGG 2 cut(s) 216, 433
AgeI ACCGGT 2 cut(s) 263, 358
AgsI TTSAA 9 cut(s) 175, 301, 461, 572, 893, 976, 1592, 1811, 2143
AhlI ACTAGT 1 cut(s) 997
AjnI CCWGG 8 cut(s) 7, 45, 215, 375, 463, 756, 1249, 1594
Alw21I GWGCWC 1 cut(s) 1716
Alw26I GTCTC 3 cut(s) 244, 254, 1705
AlwI GGATC 6 cut(s) 52, 174, 187, 247, 444, 1579
AlwNI CAGNNNCTG 1 cut(s) 1175
AoxI GGCC 6 cut(s) 43, 48, 268, 373, 754, 1853
ApeKI GCWGC 6 cut(s) 145, 860, 992, 1015, 1136, 1940
ApoI RAATTY 7 cut(s) 314, 552, 627, 674, 737, 1196, 2051
ArsI GACNNNNNNTTYG 2 cut(s) 1534, 1566
AsiGI ACCGGT 2 cut(s) 263, 358
Asp700I GAANNNNTTC 2 cut(s) 897, 1301
AspLEI GCGC 2 cut(s) 150, 230
AspS9I GGNCC 3 cut(s) 4, 754, 801
AsuC2I CCSGG 1 cut(s) 623
AsuHPI GGTGA 5 cut(s) 842, 905, 1099, 1493, 2163
AvaII GGWCC 2 cut(s) 4, 801
BaeI ACNNNNGTAYC 2 cut(s) 1238, 1271
BalI TGGCCA 2 cut(s) 50, 375
BamHI GGATCC 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 1716
BbvI GCAGC 6 cut(s) 157, 847, 1004, 1027, 1148, 1927
BccI CCATC 5 cut(s) 59, 83, 722, 1553, 2077
BciT130I CCWGG 8 cut(s) 9, 47, 217, 377, 465, 758, 1251, 1596
BclI TGATCA 1 cut(s) 601
BcnI CCSGG 1 cut(s) 623
BcoDI GTCTC 3 cut(s) 244, 254, 1705
BcuI ACTAGT 1 cut(s) 997
BfaI CTAG 6 cut(s) 617, 998, 1121, 1346, 1454, 1497
BfmI CTRYAG 1 cut(s) 367
BglII AGATCT 1 cut(s) 650
BmcAI AGTACT 1 cut(s) 351
Bme1390I CCNGG 9 cut(s) 9, 47, 217, 377, 465, 623, 758, 1251, 1596
Bme18I GGWCC 2 cut(s) 4, 801
BmgT120I GGNCC 3 cut(s) 4, 754, 801
BmiI GGNNCC 3 cut(s) 6, 181, 1053
BmrFI CCNGG 9 cut(s) 9, 47, 217, 377, 465, 623, 758, 1251, 1596
BmrI ACTGGG 2 cut(s) 162, 1163
BmsI GCATC 6 cut(s) 601, 697, 816, 847, 1603, 1952
BmuI ACTGGG 2 cut(s) 162, 1163
BpmI CTGGAG 1 cut(s) 1038
BpuEI CTTGAG 4 cut(s) 65, 278, 811, 1509
BpuMI CCSGG 1 cut(s) 623
Bsa29I ATCGAT 1 cut(s) 2195
BsaHI GRCGYC 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 8 cut(s) 7, 243, 376, 432, 1061, 1230, 1250, 2046
BsaWI WCCGGW 2 cut(s) 263, 358
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 1737, 1767
Bsc4I CCNNNNNNNGG 2 cut(s) 216, 433
Bse118I RCCGGY 2 cut(s) 263, 358
Bse1I ACTGG 3 cut(s) 168, 916, 1169
Bse3DI GCAATG 3 cut(s) 1332, 1878, 2109
BseBI CCWGG 8 cut(s) 9, 47, 217, 377, 465, 758, 1251, 1596
BseCI ATCGAT 1 cut(s) 2195
BseDI CCNNGG 8 cut(s) 7, 243, 376, 432, 1061, 1230, 1250, 2046
BseGI GGATG 9 cut(s) 75, 253, 665, 691, 1072, 1480, 1618, 1860, 1943
BseLI CCNNNNNNNGG 2 cut(s) 216, 433
BseMI GCAATG 3 cut(s) 1332, 1878, 2109
BseMII CTCAG 3 cut(s) 143, 410, 1025
BseNI ACTGG 3 cut(s) 168, 916, 1169
BseXI GCAGC 6 cut(s) 157, 847, 1004, 1027, 1148, 1927
BseYI CCCAGC 2 cut(s) 751, 1330
BsgI GTGCAG 1 cut(s) 1011
Bsh1236I CGCG 3 cut(s) 228, 237, 657
BshFI GGCC 6 cut(s) 45, 50, 270, 375, 756, 1855
BshTI ACCGGT 2 cut(s) 263, 358
BshVI ATCGAT 1 cut(s) 2195
BsiHKAI GWGCWC 1 cut(s) 1716
BsiSI CCGG 3 cut(s) 264, 359, 622
BsiWI CGTACG 1 cut(s) 96
BslFI GGGAC 1 cut(s) 2121
BslI CCNNNNNNNGG 2 cut(s) 216, 433
BsmAI GTCTC 3 cut(s) 244, 254, 1705
BsmBI CGTCTC 1 cut(s) 244
BsmFI GGGAC 1 cut(s) 2121
BsmI GAATGC 1 cut(s) 2047
BsnI GGCC 6 cut(s) 45, 50, 270, 375, 756, 1855
Bso31I GGTCTC 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 1716
Bsp1407I TGTACA 2 cut(s) 534, 1628
Bsp143I GATC 7 cut(s) 57, 179, 252, 436, 601, 650, 1584
BspANI GGCC 6 cut(s) 45, 50, 270, 375, 756, 1855
BspCNI CTCAG 3 cut(s) 142, 411, 1024
BspDI ATCGAT 1 cut(s) 2195
BspFNI CGCG 3 cut(s) 228, 237, 657
BspHI TCATGA 1 cut(s) 547
BspLI GGNNCC 3 cut(s) 6, 181, 1053
BspPI GGATC 6 cut(s) 52, 174, 187, 247, 444, 1579
BspQI GCTCTTC 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 254
BsrDI GCAATG 3 cut(s) 1332, 1878, 2109
BsrFI RCCGGY 2 cut(s) 263, 358
BsrGI TGTACA 2 cut(s) 534, 1628
BsrI ACTGG 3 cut(s) 168, 916, 1169
BssAI RCCGGY 2 cut(s) 263, 358
BssECI CCNNGG 8 cut(s) 7, 243, 376, 432, 1061, 1230, 1250, 2046
BssMI GATC 7 cut(s) 57, 179, 252, 436, 601, 650, 1584
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 4 cut(s) 432, 1061, 1230, 2046
Bst2UI CCWGG 8 cut(s) 9, 47, 217, 377, 465, 758, 1251, 1596
Bst6I CTCTTC 4 cut(s) 74, 302, 446, 1149
BstACI GRCGYC 1 cut(s) 276
BstAPI GCANNNNNTGC 1 cut(s) 2111
BstAUI TGTACA 2 cut(s) 534, 1628
BstC8I GCNNGC 8 cut(s) 31, 387, 797, 1020, 1118, 1351, 1938, 2107
BstDEI CTNAG 3 cut(s) 129, 419, 1011
BstF5I GGATG 9 cut(s) 75, 253, 665, 691, 1072, 1480, 1618, 1860, 1943
BstFNI CGCG 3 cut(s) 228, 237, 657
BstHHI GCGC 2 cut(s) 150, 230
BstKTI GATC 7 cut(s) 60, 182, 255, 439, 604, 653, 1587
BstMAI GTCTC 3 cut(s) 244, 254, 1705
BstMBI GATC 7 cut(s) 57, 179, 252, 436, 601, 650, 1584
BstMWI GCNNNNNNNGC 6 cut(s) 142, 234, 869, 1346, 1520, 2111
BstNI CCWGG 8 cut(s) 9, 47, 217, 377, 465, 758, 1251, 1596
BstNSI RCATGY 2 cut(s) 1022, 1842
BstSCI CCNGG 9 cut(s) 7, 45, 215, 375, 463, 621, 756, 1249, 1594
BstSFI CTRYAG 1 cut(s) 367
BstUI CGCG 3 cut(s) 228, 237, 657
BstV1I GCAGC 6 cut(s) 157, 847, 1004, 1027, 1148, 1927
BstX2I RGATCY 5 cut(s) 179, 252, 436, 650, 1584
BstXI CCANNNNNNTGG 1 cut(s) 1035
BstYI RGATCY 5 cut(s) 179, 252, 436, 650, 1584
Bsu15I ATCGAT 1 cut(s) 2195
BsuRI GGCC 6 cut(s) 45, 50, 270, 375, 756, 1855
BsuTUI ATCGAT 1 cut(s) 2195
BtsCI GGATG 9 cut(s) 75, 253, 665, 691, 1072, 1480, 1618, 1860, 1943
BtsI GCAGTG 1 cut(s) 1173
BtsIMutI CAGTG 4 cut(s) 376, 1173, 1703, 1953
Cac8I GCNNGC 8 cut(s) 31, 387, 797, 1020, 1118, 1351, 1938, 2107
CaiI CAGNNNCTG 1 cut(s) 1175
CciI TCATGA 1 cut(s) 547
CfoI GCGC 2 cut(s) 150, 230
Cfr10I RCCGGY 2 cut(s) 263, 358
Cfr13I GGNCC 3 cut(s) 4, 754, 801
ClaI ATCGAT 1 cut(s) 2195
CseI GACGC 3 cut(s) 226, 284, 2123
CsiI ACCWGGT 2 cut(s) 463, 1594
Csp6I GTAC 4 cut(s) 97, 350, 535, 1629
CspAI ACCGGT 2 cut(s) 263, 358
CspCI CAANNNNNGTGG 2 cut(s) 2112, 2147
CviAII CATG 9 cut(s) 495, 548, 854, 878, 1019, 1048, 1393, 1723, 1839
CviQI GTAC 4 cut(s) 97, 350, 535, 1629
DdeI CTNAG 3 cut(s) 129, 419, 1011
DpnI GATC 7 cut(s) 59, 181, 254, 438, 603, 652, 1586
DpnII GATC 7 cut(s) 57, 179, 252, 436, 601, 650, 1584
EaeI YGGCCR 2 cut(s) 48, 373
Eam1104I CTCTTC 4 cut(s) 74, 302, 446, 1149
EarI CTCTTC 4 cut(s) 74, 302, 446, 1149
Eco130I CCWWGG 4 cut(s) 432, 1061, 1230, 2046
Eco147I AGGCCT 1 cut(s) 45
Eco31I GGTCTC 1 cut(s) 254
Eco32I GATATC 1 cut(s) 1277
Eco47I GGWCC 2 cut(s) 4, 801
Eco57I CTGAAG 2 cut(s) 427, 1316
EcoRII CCWGG 8 cut(s) 7, 45, 215, 375, 463, 756, 1249, 1594
EcoRV GATATC 1 cut(s) 1277
EcoT14I CCWWGG 4 cut(s) 432, 1061, 1230, 2046
ErhI CCWWGG 4 cut(s) 432, 1061, 1230, 2046
Esp3I CGTCTC 1 cut(s) 244
FaeI CATG 9 cut(s) 498, 551, 857, 881, 1022, 1051, 1396, 1726, 1842
FalI AAGNNNNNCTT 2 cut(s) 2098, 2130
FaqI GGGAC 1 cut(s) 2121
FatI CATG 9 cut(s) 494, 547, 853, 877, 1018, 1047, 1392, 1722, 1838
FauI CCCGC 2 cut(s) 22, 200
FauNDI CATATG 2 cut(s) 697, 1633
FbaI TGATCA 1 cut(s) 601
FblI GTMKAC 1 cut(s) 108
FokI GGATG 9 cut(s) 62, 260, 672, 678, 1079, 1467, 1625, 1847, 1930
FspBI CTAG 6 cut(s) 617, 998, 1121, 1346, 1454, 1497
GlaI GCGC 2 cut(s) 149, 229
GsaI CCCAGC 2 cut(s) 755, 1334
GsuI CTGGAG 1 cut(s) 1038
HaeIII GGCC 6 cut(s) 45, 50, 270, 375, 756, 1855
HapII CCGG 3 cut(s) 264, 359, 622
HgaI GACGC 3 cut(s) 226, 284, 2123
HhaI GCGC 2 cut(s) 150, 230
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 9 cut(s) 498, 551, 857, 881, 1022, 1051, 1396, 1726, 1842
Hin6I GCGC 2 cut(s) 148, 228
HinP1I GCGC 2 cut(s) 148, 228
HindIII AAGCTT 3 cut(s) 887, 935, 1383
HinfI GANTC 7 cut(s) 568, 910, 979, 1141, 1469, 1686, 2002
HpaII CCGG 3 cut(s) 264, 359, 622
HphI GGTGA 5 cut(s) 842, 905, 1099, 1493, 2163
Hpy166II GTNNAC 5 cut(s) 109, 364, 609, 804, 1601
Hpy188I TCNGA 9 cut(s) 244, 420, 717, 928, 1296, 1415, 1926, 2082, 2158
Hpy188III TCNNGA 7 cut(s) 55, 82, 548, 682, 976, 1055, 2038
Hpy8I GTNNAC 5 cut(s) 109, 364, 609, 804, 1601
Hpy99I CGWCG 2 cut(s) 145, 278
HpyAV CCTTC 6 cut(s) 216, 643, 855, 1291, 1598, 2184
HpyCH4IV ACGT 2 cut(s) 162, 2145
HpyF10VI GCNNNNNNNGC 6 cut(s) 142, 234, 869, 1346, 1520, 2111
HpyF3I CTNAG 3 cut(s) 129, 419, 1011
HpySE526I ACGT 2 cut(s) 162, 2145
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 9 cut(s) 498, 551, 857, 881, 1022, 1051, 1396, 1726, 1842
HspAI GCGC 2 cut(s) 148, 228
Ksp22I TGATCA 1 cut(s) 601
Kzo9I GATC 7 cut(s) 57, 179, 252, 436, 601, 650, 1584
LguI GCTCTTC 1 cut(s) 302
LmnI GCTCC 3 cut(s) 1057, 1431, 1758
Lsp1109I GCAGC 6 cut(s) 157, 847, 1004, 1027, 1148, 1927
LweI GCATC 6 cut(s) 601, 697, 816, 847, 1603, 1952
MabI ACCWGGT 2 cut(s) 463, 1594
MaeI CTAG 6 cut(s) 617, 998, 1121, 1346, 1454, 1497
MaeII ACGT 2 cut(s) 162, 2145
MaeIII GTNAC 7 cut(s) 918, 951, 967, 1171, 1499, 1540, 1738
MalI GATC 7 cut(s) 59, 181, 254, 438, 603, 652, 1586
MboI GATC 7 cut(s) 57, 179, 252, 436, 601, 650, 1584
MboII GAAGA 8 cut(s) 61, 289, 433, 905, 1136, 1271, 1823, 2003
MflI RGATCY 5 cut(s) 179, 252, 436, 650, 1584
MhlI GDGCHC 1 cut(s) 1716
MlsI TGGCCA 2 cut(s) 50, 375
MluNI TGGCCA 2 cut(s) 50, 375
MlyI GAGTC 2 cut(s) 973, 1135
MmeI TCCRAC 2 cut(s) 1393, 1413
Mox20I TGGCCA 2 cut(s) 50, 375
MroXI GAANNNNTTC 2 cut(s) 897, 1301
MscI TGGCCA 2 cut(s) 50, 375
MseI TTAA 5 cut(s) 939, 1256, 1986, 2091, 2200
MslI CAYNNNNRTG 4 cut(s) 499, 696, 855, 1721
Msp20I TGGCCA 2 cut(s) 50, 375
MspA1I CMGCKG 1 cut(s) 1940
MspI CCGG 3 cut(s) 264, 359, 622
MspR9I CCNGG 9 cut(s) 9, 47, 217, 377, 465, 623, 758, 1251, 1596
Mva1269I GAATGC 1 cut(s) 2047
MvaI CCWGG 8 cut(s) 9, 47, 217, 377, 465, 758, 1251, 1596
MvnI CGCG 3 cut(s) 228, 237, 657
MwoI GCNNNNNNNGC 6 cut(s) 142, 234, 869, 1346, 1520, 2111
NciI CCSGG 1 cut(s) 623
NdeI CATATG 2 cut(s) 697, 1633
NdeII GATC 7 cut(s) 57, 179, 252, 436, 601, 650, 1584
NlaIII CATG 9 cut(s) 498, 551, 857, 881, 1022, 1051, 1396, 1726, 1842
NlaIV GGNNCC 3 cut(s) 6, 181, 1053
NmeAIII GCCGAG 1 cut(s) 2187
NmuCI GTSAC 4 cut(s) 1171, 1499, 1540, 1738
NspI RCATGY 2 cut(s) 1022, 1842
PaeI GCATGC 1 cut(s) 1022
PagI TCATGA 1 cut(s) 547
PceI AGGCCT 1 cut(s) 45
PciSI GCTCTTC 1 cut(s) 302
PctI GAATGC 1 cut(s) 2047
PdmI GAANNNNTTC 2 cut(s) 897, 1301
PfeI GAWTC 5 cut(s) 568, 910, 1469, 1686, 2002
Pfl23II CGTACG 1 cut(s) 96
PflMI CCANNNNNTGG 1 cut(s) 433
PfoI TCCNGGA 2 cut(s) 215, 621
PinAI ACCGGT 2 cut(s) 263, 358
PleI GAGTC 2 cut(s) 973, 1135
PpsI GAGTC 2 cut(s) 973, 1135
Psp1406I AACGTT 1 cut(s) 162
Psp6I CCWGG 8 cut(s) 7, 45, 215, 375, 463, 756, 1249, 1594
PspFI CCCAGC 2 cut(s) 751, 1330
PspGI CCWGG 8 cut(s) 7, 45, 215, 375, 463, 756, 1249, 1594
PspLI CGTACG 1 cut(s) 96
PspN4I GGNNCC 3 cut(s) 6, 181, 1053
PspPI GGNCC 3 cut(s) 4, 754, 801
PstNI CAGNNNCTG 1 cut(s) 1175
PsuI RGATCY 5 cut(s) 179, 252, 436, 650, 1584
PvuII CAGCTG 1 cut(s) 1940
RsaI GTAC 4 cut(s) 98, 351, 536, 1630
RsaNI GTAC 4 cut(s) 97, 350, 535, 1629
RseI CAYNNNNRTG 4 cut(s) 499, 696, 855, 1721
SapI GCTCTTC 1 cut(s) 302
SaqAI TTAA 5 cut(s) 939, 1256, 1986, 2091, 2200
Sau3AI GATC 7 cut(s) 57, 179, 252, 436, 601, 650, 1584
Sau96I GGNCC 3 cut(s) 4, 754, 801
ScaI AGTACT 1 cut(s) 351
SchI GAGTC 2 cut(s) 973, 1135
ScrFI CCNGG 9 cut(s) 9, 47, 217, 377, 465, 623, 758, 1251, 1596
SduI GDGCHC 1 cut(s) 1716
SexAI ACCWGGT 2 cut(s) 463, 1594
SfaNI GCATC 6 cut(s) 601, 697, 816, 847, 1603, 1952
SfcI CTRYAG 1 cut(s) 367
SinI GGWCC 2 cut(s) 4, 801
SmiMI CAYNNNNRTG 4 cut(s) 499, 696, 855, 1721
SmlI CTYRAG 4 cut(s) 80, 257, 790, 1524
SmoI CTYRAG 4 cut(s) 80, 257, 790, 1524
SpeI ACTAGT 1 cut(s) 997
SphI GCATGC 1 cut(s) 1022
SseBI AGGCCT 1 cut(s) 45
SspI AATATT 1 cut(s) 586
SspMI CTAG 6 cut(s) 617, 998, 1121, 1346, 1454, 1497
StuI AGGCCT 1 cut(s) 45
StyD4I CCNGG 9 cut(s) 7, 45, 215, 375, 463, 621, 756, 1249, 1594
StyI CCWWGG 4 cut(s) 432, 1061, 1230, 2046
TaiI ACGT 2 cut(s) 165, 2148
TaqI TCGA 3 cut(s) 273, 683, 2195
TaqII GACCGA 1 cut(s) 1910
TatI WGTACW 3 cut(s) 349, 534, 1628
TauI GCSGC 4 cut(s) 123, 198, 228, 399
TfiI GAWTC 5 cut(s) 568, 910, 1469, 1686, 2002
Tru1I TTAA 5 cut(s) 939, 1256, 1986, 2091, 2200
Tru9I TTAA 5 cut(s) 939, 1256, 1986, 2091, 2200
TscAI CASTG 4 cut(s) 376, 1180, 1710, 1960
TseFI GTSAC 4 cut(s) 1171, 1499, 1540, 1738
TseI GCWGC 6 cut(s) 145, 860, 992, 1015, 1136, 1940
Tsp45I GTSAC 4 cut(s) 1171, 1499, 1540, 1738
TspGWI ACGGA 1 cut(s) 418
TspRI CASTG 4 cut(s) 376, 1180, 1710, 1960
Van91I CCANNNNNTGG 1 cut(s) 433
VpaK11BI GGWCC 2 cut(s) 4, 801
XapI RAATTY 7 cut(s) 314, 552, 627, 674, 737, 1196, 2051
XceI RCATGY 2 cut(s) 1022, 1842
XmiI GTMKAC 1 cut(s) 108
XmnI GAANNNNTTC 2 cut(s) 897, 1301
XspI CTAG 6 cut(s) 617, 998, 1121, 1346, 1454, 1497
ZrmI AGTACT 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.