MD02G1292300.v1.1

Calcium-dependent channel, 7TM region, putative phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005105
Physical Location & Seq
Reverse (-)
34826024 .. 34830311
4288 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1292300.v1.1.491

Sequence Viewer

Length: 1269 bp
ATGATTTTCTCCCTAGTTATCCACTCCCAGCAGCACTCAAATCCACTTCTTTGGATCACTGACGATGCTTCAGAACCCAGGGATGTATCATGGAGAAATTTGGCAATCCCAGTCCGAGTTTTGCCACTCTGCAATTTTGGAGTTATTGTTGCAGCCTCCCTTCTTACAATTTTCTTTGCTATCCCAGTCACTGCCGTCCAGGGAATTGCCAAATTTAAAAAGTTAAAGAAATGGTTTCCCCCTGCCATGGCTTTACAGTTGATATCAGGGTTAAGCTCTGTCATAACAGGGTATCTTCCGAGTGCCGTTCTGAATTTGTTTATCTACATTGTTCCATTTACAATGCTCGGAATGGCTAAACTATCAGGCTGTATCTCAAAGAGCAGGGAGGAGATAAAAGCGTGCAACATGGTTTTATATTTCCTAGTTGGAAACGTCTTCTTTTGGAGCTTGTTATCTGGCTCTTTAATAGATGAAATTGGCGAATCTTTTACTCATCCGAAAGATATCCCCAATCACTTTGCTAGAGCTGTCTCTGCTCAAGCAGATTTCTTTGTGACATATACTTTGACAGATGGGCTGTCGGGGTTTTCTTTGGAGATCCTCCAACCAGGATTACTTTGCTGGGATGCTATAAGGTCCTGTACATATGGCCGTGGAAAAGAGAAGACTCCTTATCTTTATTCATTACCTTACTTCAGAATCATTCCTGTGGTCTCGCTGTCCGTGCTGATTGGTACAGTATATGCCGTTGTGGCACCACTTATGCTCCCATTTTTGATTGGTTACTTCTTGCTCGGCTATGCGGTGTATATAAACCAGATTGAAAATGTTTATGAGACTGTTTATGATACGTGTGGACAGTATTGGCCTTACATCAATCATTATATTCTCGTTGCAATCATTCTCATGCAAATAACTATGATTGGTCTCTATGGACTGAAATCAAAGCCGGCCGCATCCTTTTCCACAATTCCACTTCTAGTGCTGACATTGATGTTTAATGATTACTGCAAGATGCGTTTTCTTCCTGCATTCCACCTTTTATCTATTCAGAATGCCAAGGAATTTGATGAACTTGATGAGAAGTGCAATCAGGTGGAGTTTAACTGTGAGAATGCTAGCACTGCTTATTGCCCCCCTTGTTTGCGTCCTTTGAATTGCATGGAATCAGGGTCAAGCCACACGCCGCCTTTAGTTATTCTTCGTCATTATAAACTCAAGCTCCCCCAGTACTCGTCTTTTTTTGATGTGGAGAATCAGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

423

Amino Acids

47.43

Weight (kDa)

7.04

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_7TM PF02714 46 - 311 2.4e-71 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1215
AccB1I GGYRCC 1 cut(s) 757
AciI CCGC 3 cut(s) 806, 957, 1190
AclWI GGATC 2 cut(s) 62, 595
AcoI YGGCCR 2 cut(s) 652, 954
AcsI RAATTY 4 cut(s) 97, 212, 313, 1067
AcuI CTGAAG 2 cut(s) 54, 682
AfaI GTAC 3 cut(s) 646, 739, 1235
AfiI CCNNNNNNNGG 1 cut(s) 247
AflIII ACRYGT 1 cut(s) 854
AgsI TTSAA 2 cut(s) 827, 1159
AjnI CCWGG 3 cut(s) 77, 198, 610
AluBI AGCT 4 cut(s) 276, 450, 530, 1225
AluI AGCT 4 cut(s) 276, 450, 530, 1225
Alw26I GTCTC 4 cut(s) 538, 721, 833, 935
AlwI GGATC 2 cut(s) 62, 595
AlwNI CAGNNNCTG 1 cut(s) 191
AoxI GGCC 3 cut(s) 652, 869, 954
ApeKI GCWGC 2 cut(s) 31, 152
ApoI RAATTY 4 cut(s) 97, 212, 313, 1067
ArsI GACNNNNNNTTYG 2 cut(s) 550, 582
AspS9I GGNCC 1 cut(s) 639
AsuNHI GCTAGC 1 cut(s) 1121
AvaII GGWCC 1 cut(s) 639
BanI GGYRCC 1 cut(s) 757
BbsI GAAGAC 2 cut(s) 430, 674
BbvI GCAGC 2 cut(s) 43, 164
BccI CCATC 1 cut(s) 569
BceAI ACGGC 4 cut(s) 179, 290, 639, 734
BciT130I CCWGG 3 cut(s) 79, 200, 612
BcoDI GTCTC 4 cut(s) 538, 721, 833, 935
BfaI CTAG 5 cut(s) 14, 425, 525, 983, 1122
BglI GCCNNNNNGGC 1 cut(s) 755
BisI GCNGC 4 cut(s) 32, 153, 957, 1190
BlsI GCNGC 4 cut(s) 33, 154, 958, 1191
BmcAI AGTACT 1 cut(s) 1235
Bme1390I CCNGG 3 cut(s) 79, 200, 612
Bme18I GGWCC 1 cut(s) 639
BmgT120I GGNCC 1 cut(s) 639
BmiI GGNNCC 1 cut(s) 759
BmrFI CCNGG 3 cut(s) 79, 200, 612
BmrI ACTGGG 3 cut(s) 104, 179, 1225
BmsI GCATC 4 cut(s) 55, 619, 968, 1008
BmtI GCTAGC 1 cut(s) 1125
BmuI ACTGGG 3 cut(s) 104, 179, 1225
BpiI GAAGAC 2 cut(s) 430, 674
BpuEI CTTGAG 2 cut(s) 525, 1205
BsaAI YACGTR 1 cut(s) 855
BsaI GGTCTC 2 cut(s) 721, 935
BsaJI CCNNGG 6 cut(s) 77, 78, 199, 246, 655, 1062
BsaXI ACNNNNNCTCC 6 cut(s) 132, 162, 753, 783, 1209, 1239
Bsc4I CCNNNNNNNGG 1 cut(s) 247
Bse118I RCCGGY 1 cut(s) 952
Bse1I ACTGG 3 cut(s) 110, 185, 1231
BseBI CCWGG 3 cut(s) 79, 200, 612
BseDI CCNNGG 6 cut(s) 77, 78, 199, 246, 655, 1062
BseGI GGATG 4 cut(s) 88, 496, 634, 959
BseLI CCNNNNNNNGG 1 cut(s) 247
BseNI ACTGG 3 cut(s) 110, 185, 1231
BseRI GAGGAG 1 cut(s) 404
BseX3I CGGCCG 1 cut(s) 954
BseXI GCAGC 2 cut(s) 43, 164
BseYI CCCAGC 2 cut(s) 27, 624
Bsh1285I CGRYCG 1 cut(s) 957
BshFI GGCC 3 cut(s) 654, 871, 956
BshNI GGYRCC 1 cut(s) 757
BsiEI CGRYCG 1 cut(s) 957
BsiSI CCGG 1 cut(s) 953
BslI CCNNNNNNNGG 1 cut(s) 247
BsmAI GTCTC 4 cut(s) 538, 721, 833, 935
BsmI GAATGC 3 cut(s) 1034, 1063, 1123
BsnI GGCC 3 cut(s) 654, 871, 956
Bso31I GGTCTC 2 cut(s) 721, 935
Bsp1407I TGTACA 1 cut(s) 644
Bsp143I GATC 2 cut(s) 54, 600
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 3 cut(s) 806, 957, 1190
BspANI GGCC 3 cut(s) 654, 871, 956
BspLI GGNNCC 1 cut(s) 759
BspOI GCTAGC 1 cut(s) 1125
BspPI GGATC 2 cut(s) 62, 595
BspT107I GGYRCC 1 cut(s) 757
BspTNI GGTCTC 2 cut(s) 721, 935
BsrFI RCCGGY 1 cut(s) 952
BsrGI TGTACA 1 cut(s) 644
BsrI ACTGG 3 cut(s) 110, 185, 1231
BssAI RCCGGY 1 cut(s) 952
BssECI CCNNGG 6 cut(s) 77, 78, 199, 246, 655, 1062
BssMI GATC 2 cut(s) 54, 600
BssT1I CCWWGG 2 cut(s) 246, 1062
Bst2UI CCWGG 3 cut(s) 79, 200, 612
Bst4CI ACNGT 5 cut(s) 258, 742, 844, 864, 1112
BstAUI TGTACA 1 cut(s) 644
BstBAI YACGTR 1 cut(s) 855
BstC8I GCNNGC 3 cut(s) 403, 954, 1123
BstDSI CCRYGG 2 cut(s) 246, 655
BstF5I GGATG 4 cut(s) 88, 496, 634, 959
BstKTI GATC 2 cut(s) 57, 603
BstMAI GTCTC 4 cut(s) 538, 721, 833, 935
BstMBI GATC 2 cut(s) 54, 600
BstMCI CGRYCG 1 cut(s) 957
BstMWI GCNNNNNNNGC 4 cut(s) 536, 727, 755, 1127
BstNI CCWGG 3 cut(s) 79, 200, 612
BstSCI CCNGG 3 cut(s) 77, 198, 610
BstV1I GCAGC 2 cut(s) 43, 164
BstV2I GAAGAC 2 cut(s) 430, 674
BstX2I RGATCY 1 cut(s) 600
BstXI CCANNNNNNTGG 1 cut(s) 51
BstYI RGATCY 1 cut(s) 600
BstZI CGGCCG 1 cut(s) 954
BsuRI GGCC 3 cut(s) 654, 871, 956
BtgI CCRYGG 2 cut(s) 246, 655
BtsCI GGATG 4 cut(s) 88, 496, 634, 959
BtsI GCAGTG 2 cut(s) 189, 1125
BtsIMutI CAGTG 3 cut(s) 57, 189, 1125
Cac8I GCNNGC 3 cut(s) 403, 954, 1123
CaiI CAGNNNCTG 1 cut(s) 191
Cfr10I RCCGGY 1 cut(s) 952
Cfr13I GGNCC 1 cut(s) 639
CseI GACGC 1 cut(s) 1139
Csp6I GTAC 3 cut(s) 645, 738, 1234
CviAII CATG 5 cut(s) 90, 247, 409, 910, 1165
CviQI GTAC 3 cut(s) 645, 738, 1234
DpnI GATC 2 cut(s) 56, 602
DpnII GATC 2 cut(s) 54, 600
DraI TTTAAA 1 cut(s) 217
EaeI YGGCCR 2 cut(s) 652, 954
EagI CGGCCG 1 cut(s) 954
EclXI CGGCCG 1 cut(s) 954
Eco130I CCWWGG 2 cut(s) 246, 1062
Eco31I GGTCTC 2 cut(s) 721, 935
Eco32I GATATC 2 cut(s) 264, 508
Eco47I GGWCC 1 cut(s) 639
Eco52I CGGCCG 1 cut(s) 954
Eco57I CTGAAG 2 cut(s) 54, 682
EcoO109I RGGNCCY 1 cut(s) 639
EcoRII CCWGG 3 cut(s) 77, 198, 610
EcoRV GATATC 2 cut(s) 264, 508
EcoT14I CCWWGG 2 cut(s) 246, 1062
ErhI CCWWGG 2 cut(s) 246, 1062
FaeI CATG 5 cut(s) 93, 250, 412, 913, 1168
FatI CATG 5 cut(s) 89, 246, 408, 909, 1164
FauNDI CATATG 1 cut(s) 649
Fnu4HI GCNGC 4 cut(s) 32, 153, 957, 1190
FokI GGATG 4 cut(s) 95, 483, 641, 946
Fsp4HI GCNGC 4 cut(s) 32, 153, 957, 1190
FspBI CTAG 5 cut(s) 14, 425, 525, 983, 1122
GluI GCNGC 4 cut(s) 32, 153, 957, 1190
GsaI CCCAGC 2 cut(s) 31, 628
HaeIII GGCC 3 cut(s) 654, 871, 956
HapII CCGG 1 cut(s) 953
HgaI GACGC 1 cut(s) 1139
Hin1II CATG 5 cut(s) 93, 250, 412, 913, 1168
HinfI GANTC 5 cut(s) 485, 670, 702, 1169, 1258
HpaII CCGG 1 cut(s) 953
Hpy166II GTNNAC 1 cut(s) 860
Hpy188I TCNGA 8 cut(s) 73, 116, 300, 312, 350, 501, 701, 1056
Hpy8I GTNNAC 1 cut(s) 860
HpyAV CCTTC 1 cut(s) 170
HpyCH4III ACNGT 5 cut(s) 258, 742, 844, 864, 1112
HpyCH4IV ACGT 2 cut(s) 435, 854
HpyCH4V TGCA 9 cut(s) 132, 152, 405, 899, 913, 1014, 1034, 1092, 1164
HpyF10VI GCNNNNNNNGC 4 cut(s) 536, 727, 755, 1127
HpySE526I ACGT 2 cut(s) 435, 854
Hsp92II CATG 5 cut(s) 93, 250, 412, 913, 1168
KroI GCCGGC 1 cut(s) 952
KroNI GCCGGC 1 cut(s) 954
Kzo9I GATC 2 cut(s) 54, 600
LmnI GCTCC 3 cut(s) 447, 774, 1230
Lsp1109I GCAGC 2 cut(s) 43, 164
LweI GCATC 4 cut(s) 55, 619, 968, 1008
MaeI CTAG 5 cut(s) 14, 425, 525, 983, 1122
MaeII ACGT 2 cut(s) 435, 854
MaeIII GTNAC 3 cut(s) 187, 556, 785
MalI GATC 2 cut(s) 56, 602
MboI GATC 2 cut(s) 54, 600
MboII GAAGA 5 cut(s) 287, 430, 679, 1019, 1196
MflI RGATCY 1 cut(s) 600
MlyI GAGTC 1 cut(s) 664
MmeI TCCRAC 2 cut(s) 409, 631
MnlI CCTC 3 cut(s) 166, 382, 614
MroNI GCCGGC 1 cut(s) 952
MseI TTAA 6 cut(s) 216, 224, 272, 467, 1002, 1107
MslI CAYNNNNRTG 2 cut(s) 710, 908
MspI CCGG 1 cut(s) 953
MspR9I CCNGG 3 cut(s) 79, 200, 612
Mva1269I GAATGC 3 cut(s) 1034, 1063, 1123
MvaI CCWGG 3 cut(s) 79, 200, 612
MwoI GCNNNNNNNGC 4 cut(s) 536, 727, 755, 1127
NaeI GCCGGC 1 cut(s) 954
NcoI CCATGG 1 cut(s) 246
NdeI CATATG 1 cut(s) 649
NdeII GATC 2 cut(s) 54, 600
NgoMIV GCCGGC 1 cut(s) 952
NheI GCTAGC 1 cut(s) 1121
NlaIII CATG 5 cut(s) 93, 250, 412, 913, 1168
NlaIV GGNNCC 1 cut(s) 759
NmeAIII GCCGAG 1 cut(s) 777
NmuCI GTSAC 2 cut(s) 187, 556
PasI CCCWGGG 1 cut(s) 78
PctI GAATGC 3 cut(s) 1034, 1063, 1123
PdiI GCCGGC 1 cut(s) 954
PfeI GAWTC 4 cut(s) 485, 702, 1169, 1258
PkrI GCNGC 4 cut(s) 33, 154, 958, 1191
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
Ppu21I YACGTR 1 cut(s) 855
PpuMI RGGWCCY 1 cut(s) 639
PsiI TTATAA 1 cut(s) 1215
Psp5II RGGWCCY 1 cut(s) 639
Psp6I CCWGG 3 cut(s) 77, 198, 610
PspFI CCCAGC 2 cut(s) 27, 624
PspGI CCWGG 3 cut(s) 77, 198, 610
PspN4I GGNNCC 1 cut(s) 759
PspPI GGNCC 1 cut(s) 639
PspPPI RGGWCCY 1 cut(s) 639
PstNI CAGNNNCTG 1 cut(s) 191
PsuI RGATCY 1 cut(s) 600
RsaI GTAC 3 cut(s) 646, 739, 1235
RsaNI GTAC 3 cut(s) 645, 738, 1234
RseI CAYNNNNRTG 2 cut(s) 710, 908
SaqAI TTAA 6 cut(s) 216, 224, 272, 467, 1002, 1107
SatI GCNGC 4 cut(s) 32, 153, 957, 1190
Sau3AI GATC 2 cut(s) 54, 600
Sau96I GGNCC 1 cut(s) 639
ScaI AGTACT 1 cut(s) 1235
SchI GAGTC 1 cut(s) 664
ScrFI CCNGG 3 cut(s) 79, 200, 612
SfaNI GCATC 4 cut(s) 55, 619, 968, 1008
SinI GGWCC 1 cut(s) 639
SmiMI CAYNNNNRTG 2 cut(s) 710, 908
SmlI CTYRAG 2 cut(s) 540, 1220
SmoI CTYRAG 2 cut(s) 540, 1220
SsiI CCGC 3 cut(s) 806, 957, 1190
SspMI CTAG 5 cut(s) 14, 425, 525, 983, 1122
StyD4I CCNGG 3 cut(s) 77, 198, 610
StyI CCWWGG 2 cut(s) 246, 1062
TaaI ACNGT 5 cut(s) 258, 742, 844, 864, 1112
TaiI ACGT 2 cut(s) 438, 857
TatI WGTACW 2 cut(s) 644, 1233
TauI GCSGC 2 cut(s) 959, 1192
TfiI GAWTC 4 cut(s) 485, 702, 1169, 1258
Tru1I TTAA 6 cut(s) 216, 224, 272, 467, 1002, 1107
Tru9I TTAA 6 cut(s) 216, 224, 272, 467, 1002, 1107
TscAI CASTG 3 cut(s) 64, 196, 1132
TseFI GTSAC 2 cut(s) 187, 556
TseI GCWGC 2 cut(s) 31, 152
Tsp45I GTSAC 2 cut(s) 187, 556
TspDTI ATGAA 3 cut(s) 489, 675, 1089
TspGWI ACGGA 1 cut(s) 715
TspRI CASTG 3 cut(s) 64, 196, 1132
VpaK11BI GGWCC 1 cut(s) 639
XapI RAATTY 4 cut(s) 97, 212, 313, 1067
XspI CTAG 5 cut(s) 14, 425, 525, 983, 1122
ZrmI AGTACT 1 cut(s) 1235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.