Rroxscaffold_160G00433760

Calcium-dependent channel, 7TM region, putative phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000160
OG0005105
Physical Location & Seq
Forward (+)
251533 .. 259640
8108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_160G00433760.1

Sequence Viewer

Length: 2211 bp
ATGAACCCTGAAAGTCTGATAGCGTCGGCGGCCATAAACATAGGCCTGGCCATCGTGATTCTCTCACTCTTCTCCATCCTGAAGAAGCAACCCTCGTACGCCCCTATCTACTACCCTCGCCGCCTCTCTCAGTCGCAGTCATTGCAGCCGTCGCAGCGCCACCGCCAAAACATCCCGGATTTCGATGAAGCCTTCACTGTCCGCCGCTTCCTCCCTTCCCTCTCCTGGATTCGCCGCGCCTACCGCGTCTCGGAGGATGAGATCCTCGAGACCAGTGGCCTCGACGCCCTCATCATCATCAGGCTCTTCAAGTTTGGAATCAAATTCTTTTTGGTTTGCTCTCTTATTGGATTGGCAGTACTTGTTCCAGTAAACTACTGTGGCCAGGGTCATCTCATAGCCCACCAGAGTCATCTAGCTGTGAGCCACCATTCCTTGGATCTCTTCAGTATATCTAACATCAGTCCAGGTTCTAACAGACTTGTAGAGTTGGAATATAATGAGATTTTGTCTAAAAGGATTCAACAACTTCGTAACATTAGGCATCAGCCTGATCAGTTCACTGTTCTAGTCCGGGAAATTCCCTTCTGCAATGATCACAGATCTCGCGGATGTTGTGTTGATAAATTCTTCTCTAAGCATCATCCATATGCTTATCATTCTTATCAGATTTTGTACGATGGAAAAGAATTTCAGGAATTGCTGGGCCAGGCAAAATCCATTGAAAGAAAGATAGAGGACTTGAGCAAGCGGTCCATGGGAAAGAAACAAAACGAGACCATGGGAAATAAGAAACATAATGAGACGCCTTTGCTCTCTAATCATTCTCATCAAGATGCTGCTAAATTAGCTTCACATGAGGAAAAGCTTGAAGAGCTTCATCAAAAGATACACCAGTTACAGTCTGAAAATATGCTTAAAGAAAAGGAGTTACCTGTTGCTTTTGTTACATTCAAGTCTCGGTTGGGTGCAGCACTAGTTTCCCAATCTCAGCAGCATGCAGATCCACTTCTTTGGATAACTGACATGGCTCCAGAACCGAGGGATGTATCTTGGAGAAATCTGGTGATACCATACAGAGTTTTGCCACTTTGCAGGCTAGGTGTCATTGTTGCAGCCTCTCTTCTTACAATTTTCTTTGCAATCCCAGTCACTGCTGTCCAGGGTATAGCCAAGTTTGAGAAACTAAAAAAATGGTTTCCTCCAGCCATGGCTTTACAGTTGATACCAGGGTTAAGCTCTATCATAACAGGATATCTTCCAAGTGCTGTTCTGAAAGGGTTCATATACATTGTTCCTTTTGCAATGCTTGGAATGGCTAAACTAGCTGGCTGTATATCCAAAAGCAAGGAGGAAATAAAAGCTTCCAACATGGTTTTTTATTTTTTGGTTGGAAATGTGTTCTTTTGGAGCTTGTTATCTGGGTCTTTACTAGATGAAATTGGGCAATCTTTTACTCATCCGAAAGATATTCCTGGTCACCTTGCTACTGCTGTTTCTGCTCAAGCAGACTTCTTTGTGACATATATTTTGACAGATGGACTGTCAGGGTTTTCTTTGGAGATCCTTCAACCAGGTTTACTTTGTTGGGATGCTATAAAGTCTTGTACATATGGCCGGGGGAAAGAGAAAACTCCTTATCTTTACTCATTACCTTACTACAGAATCATTCCTTCAGTCTCACTGTCTGTGCTGATTGGCATGGTTGATATTCTAACTTTGCTTTTAAAATCCAAGTTTGTTATTGTTATTATTCTCATCGTTGTTGGTGTTGGTATTCGTATCGGGATTGAGATTGAGATTGAAGTAGCAGTAATTGAAGATGTGTATGAAACAGTTTATGACACATGTGGACAGTACTGGCCATACATCCACCATTATATTCTCATCGCACTCGTTCTTATGCAAATTACAATGATTGGTCTTTTCGGTCTGAAATCAAAGCCAGCTGCATCCATTTCCACAATTCCACTTCTCTTGCTGACATTGATGTTTAATGAATATTGCAAGATTCATTTTCTTCCTACATTCCACCTTTTATCCATTCAGAATGCCAAGGAATTTGATGAACTTGATGTGAAGTGCAATGAGATGGAGTTTAACCATGAGAGTGCCAGCATTGCTTATTGCCCACCTTGTTTGCGTCCCTTGAACGTGATGGAATCAGAGCCAAGCTATTCACCTTCCATAGTTTCTACATCATCGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

736

Amino Acids

82.95

Weight (kDa)

8.07

Isoelectric Point (pI)

48.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 6 - 161 1.8e-30 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 186 - 354 4.2e-26 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 367 - 643 8.1e-57 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 436
AccII CGCG 3 cut(s) 237, 246, 609
AciI CCGC 9 cut(s) 29, 121, 163, 202, 205, 235, 244, 609, 751
AclWI GGATC 4 cut(s) 256, 447, 998, 1558
AcoI YGGCCR 5 cut(s) 30, 48, 382, 1615, 1862
AcsI RAATTY 5 cut(s) 323, 579, 626, 689, 2060
AcuI CTGAAG 3 cut(s) 101, 430, 1659
AcyI GRCGYC 2 cut(s) 285, 806
AfaI GTAC 5 cut(s) 98, 360, 677, 1609, 1859
AfiI CCNNNNNNNGG 3 cut(s) 225, 250, 436
AflIII ACRYGT 1 cut(s) 1847
AgsI TTSAA 9 cut(s) 310, 524, 725, 872, 955, 1571, 1805, 1820, 2152
AhlI ACTAGT 1 cut(s) 976
AjnI CCWGG 9 cut(s) 45, 224, 384, 466, 708, 1161, 1228, 1474, 1573
Alw26I GTCTC 6 cut(s) 253, 263, 770, 797, 963, 1684
AlwI GGATC 4 cut(s) 256, 447, 998, 1558
AlwNI CAGNNNCTG 1 cut(s) 1154
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 8 cut(s) 30, 43, 48, 277, 382, 706, 1615, 1862
ApeKI GCWGC 7 cut(s) 145, 154, 839, 971, 994, 1115, 1949
ApoI RAATTY 5 cut(s) 323, 579, 626, 689, 2060
ArsI GACNNNNNNTTYG 2 cut(s) 1513, 1545
Asp700I GAANNNNTTC 2 cut(s) 876, 1280
AspLEI GCGC 2 cut(s) 159, 239
AspS9I GGNCC 2 cut(s) 706, 753
AsuC2I CCSGG 3 cut(s) 176, 575, 1619
AsuHPI GGTGA 3 cut(s) 1078, 1472, 2172
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 1 cut(s) 753
BaeI ACNNNNGTAYC 4 cut(s) 881, 914, 1217, 1250
BalI TGGCCA 3 cut(s) 50, 384, 1864
BbvI GCAGC 7 cut(s) 157, 166, 826, 983, 1006, 1127, 1936
BccI CCATC 6 cut(s) 59, 83, 674, 1532, 2086, 2152
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 9 cut(s) 47, 226, 386, 468, 710, 1163, 1230, 1476, 1575
BclI TGATCA 2 cut(s) 553, 595
BcnI CCSGG 3 cut(s) 176, 575, 1619
BcoDI GTCTC 6 cut(s) 253, 263, 770, 797, 963, 1684
BcuI ACTAGT 1 cut(s) 976
BfaI CTAG 6 cut(s) 416, 569, 977, 1100, 1325, 1433
BfmI CTRYAG 1 cut(s) 1660
BfoI RGCGCY 1 cut(s) 160
BglII AGATCT 1 cut(s) 602
BmcAI AGTACT 2 cut(s) 360, 1859
Bme18I GGWCC 1 cut(s) 753
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 2 cut(s) 706, 753
BmiI GGNNCC 1 cut(s) 1032
BmrI ACTGGG 1 cut(s) 1142
BmsI GCATC 5 cut(s) 553, 649, 826, 1582, 1961
BmuI ACTGGG 1 cut(s) 1142
BpmI CTGGAG 2 cut(s) 1017, 1188
BpuEI CTTGAG 2 cut(s) 763, 1488
BpuMI CCSGG 3 cut(s) 176, 575, 1619
Bsa29I ATCGAT 1 cut(s) 2204
BsaHI GRCGYC 2 cut(s) 285, 806
BsaI GGTCTC 2 cut(s) 263, 770
Bsc4I CCNNNNNNNGG 3 cut(s) 225, 250, 436
Bse1I ACTGG 5 cut(s) 273, 368, 895, 1148, 1865
Bse3DI GCAATG 5 cut(s) 140, 598, 1311, 2092, 2118
BseBI CCWGG 9 cut(s) 47, 226, 386, 468, 710, 1163, 1230, 1476, 1575
BseCI ATCGAT 1 cut(s) 2204
BseLI CCNNNNNNNGG 3 cut(s) 225, 250, 436
BseMI GCAATG 5 cut(s) 140, 598, 1311, 2092, 2118
BseMII CTCAG 2 cut(s) 143, 1004
BseNI ACTGG 5 cut(s) 273, 368, 895, 1148, 1865
BseXI GCAGC 7 cut(s) 157, 166, 826, 983, 1006, 1127, 1936
BseYI CCCAGC 1 cut(s) 703
BsgI GTGCAG 1 cut(s) 990
Bsh1236I CGCG 3 cut(s) 237, 246, 609
BshFI GGCC 8 cut(s) 32, 45, 50, 279, 384, 708, 1617, 1864
BshVI ATCGAT 1 cut(s) 2204
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 3 cut(s) 176, 574, 1618
BsiWI CGTACG 1 cut(s) 96
BslFI GGGAC 1 cut(s) 2130
BslI CCNNNNNNNGG 3 cut(s) 225, 250, 436
BsmAI GTCTC 6 cut(s) 253, 263, 770, 797, 963, 1684
BsmBI CGTCTC 2 cut(s) 253, 797
BsmFI GGGAC 1 cut(s) 2130
BsmI GAATGC 1 cut(s) 2056
BsnI GGCC 8 cut(s) 32, 45, 50, 279, 384, 708, 1617, 1864
Bso31I GGTCTC 2 cut(s) 263, 770
BsoBI CYCGRG 1 cut(s) 266
Bsp1407I TGTACA 1 cut(s) 1607
Bsp143I GATC 7 cut(s) 261, 439, 553, 595, 602, 1003, 1563
Bsp19I CCATGG 3 cut(s) 756, 780, 1209
BspACI CCGC 9 cut(s) 29, 121, 163, 202, 205, 235, 244, 609, 751
BspANI GGCC 8 cut(s) 32, 45, 50, 279, 384, 708, 1617, 1864
BspCNI CTCAG 2 cut(s) 142, 1003
BspDI ATCGAT 1 cut(s) 2204
BspFNI CGCG 3 cut(s) 237, 246, 609
BspLI GGNNCC 1 cut(s) 1032
BspPI GGATC 4 cut(s) 256, 447, 998, 1558
BspQI GCTCTTC 2 cut(s) 311, 867
BspTNI GGTCTC 2 cut(s) 263, 770
BsrDI GCAATG 5 cut(s) 140, 598, 1311, 2092, 2118
BsrGI TGTACA 1 cut(s) 1607
BsrI ACTGG 5 cut(s) 273, 368, 895, 1148, 1865
BssMI GATC 7 cut(s) 261, 439, 553, 595, 602, 1003, 1563
BssNI GRCGYC 2 cut(s) 285, 806
BssT1I CCWWGG 5 cut(s) 435, 756, 780, 1209, 2055
Bst2UI CCWGG 9 cut(s) 47, 226, 386, 468, 710, 1163, 1230, 1476, 1575
Bst4CI ACNGT 9 cut(s) 199, 380, 565, 903, 1221, 1545, 1686, 1837, 1857
Bst6I CTCTTC 5 cut(s) 74, 311, 449, 867, 1128
BstACI GRCGYC 2 cut(s) 285, 806
BstAPI GCANNNNNTGC 1 cut(s) 142
BstAUI TGTACA 1 cut(s) 1607
BstC8I GCNNGC 6 cut(s) 749, 999, 1097, 1330, 1947, 2116
BstDEI CTNAG 3 cut(s) 129, 636, 990
BstDSI CCRYGG 3 cut(s) 756, 780, 1209
BstEII GGTNACC 1 cut(s) 1478
BstFNI CGCG 3 cut(s) 237, 246, 609
BstH2I RGCGCY 1 cut(s) 160
BstHHI GCGC 2 cut(s) 159, 239
BstKTI GATC 7 cut(s) 264, 442, 556, 598, 605, 1006, 1566
BstMAI GTCTC 6 cut(s) 253, 263, 770, 797, 963, 1684
BstMBI GATC 7 cut(s) 261, 439, 553, 595, 602, 1003, 1563
BstNI CCWGG 9 cut(s) 47, 226, 386, 468, 710, 1163, 1230, 1476, 1575
BstNSI RCATGY 2 cut(s) 1001, 1851
BstPI GGTNACC 1 cut(s) 1478
BstSFI CTRYAG 1 cut(s) 1660
BstUI CGCG 3 cut(s) 237, 246, 609
BstV1I GCAGC 7 cut(s) 157, 166, 826, 983, 1006, 1127, 1936
BstX2I RGATCY 5 cut(s) 261, 439, 602, 1003, 1563
BstXI CCANNNNNNTGG 1 cut(s) 1014
BstYI RGATCY 5 cut(s) 261, 439, 602, 1003, 1563
Bsu15I ATCGAT 1 cut(s) 2204
BsuRI GGCC 8 cut(s) 32, 45, 50, 279, 384, 708, 1617, 1864
BsuTUI ATCGAT 1 cut(s) 2204
BtgI CCRYGG 3 cut(s) 756, 780, 1209
BtgZI GCGATG 1 cut(s) 1873
BtsI GCAGTG 1 cut(s) 1152
BtsIMutI CAGTG 5 cut(s) 195, 280, 561, 1152, 1682
Cac8I GCNNGC 6 cut(s) 749, 999, 1097, 1330, 1947, 2116
CaiI CAGNNNCTG 1 cut(s) 1154
CfoI GCGC 2 cut(s) 159, 239
Cfr13I GGNCC 2 cut(s) 706, 753
ClaI ATCGAT 1 cut(s) 2204
CseI GACGC 5 cut(s) 12, 235, 293, 814, 2132
CsiI ACCWGGT 1 cut(s) 1573
Csp6I GTAC 5 cut(s) 97, 359, 676, 1608, 1858
CspCI CAANNNNNGTGG 4 cut(s) 1959, 1994, 2121, 2156
CviQI GTAC 5 cut(s) 97, 359, 676, 1608, 1858
DdeI CTNAG 3 cut(s) 129, 636, 990
DpnI GATC 7 cut(s) 263, 441, 555, 597, 604, 1005, 1565
DpnII GATC 7 cut(s) 261, 439, 553, 595, 602, 1003, 1563
DraI TTTAAA 1 cut(s) 1728
EaeI YGGCCR 5 cut(s) 30, 48, 382, 1615, 1862
Eam1104I CTCTTC 5 cut(s) 74, 311, 449, 867, 1128
EarI CTCTTC 5 cut(s) 74, 311, 449, 867, 1128
EciI GGCGGA 1 cut(s) 191
Eco130I CCWWGG 5 cut(s) 435, 756, 780, 1209, 2055
Eco147I AGGCCT 1 cut(s) 45
Eco31I GGTCTC 2 cut(s) 263, 770
Eco32I GATATC 1 cut(s) 1256
Eco47I GGWCC 1 cut(s) 753
Eco57I CTGAAG 3 cut(s) 101, 430, 1659
Eco88I CYCGRG 1 cut(s) 266
Eco91I GGTNACC 1 cut(s) 1478
EcoO65I GGTNACC 1 cut(s) 1478
EcoRII CCWGG 9 cut(s) 45, 224, 384, 466, 708, 1161, 1228, 1474, 1573
EcoRV GATATC 1 cut(s) 1256
EcoT14I CCWWGG 5 cut(s) 435, 756, 780, 1209, 2055
ErhI CCWWGG 5 cut(s) 435, 756, 780, 1209, 2055
Esp3I CGTCTC 2 cut(s) 253, 797
FaqI GGGAC 1 cut(s) 2130
FauNDI CATATG 2 cut(s) 649, 1612
FbaI TGATCA 2 cut(s) 553, 595
FspBI CTAG 6 cut(s) 416, 569, 977, 1100, 1325, 1433
GlaI GCGC 2 cut(s) 158, 238
GsaI CCCAGC 1 cut(s) 707
GsuI CTGGAG 2 cut(s) 1017, 1188
HaeII RGCGCY 1 cut(s) 160
HaeIII GGCC 8 cut(s) 32, 45, 50, 279, 384, 708, 1617, 1864
HapII CCGG 3 cut(s) 176, 574, 1618
HgaI GACGC 5 cut(s) 12, 235, 293, 814, 2132
HhaI GCGC 2 cut(s) 159, 239
Hin1I GRCGYC 2 cut(s) 285, 806
Hin6I GCGC 2 cut(s) 157, 237
HinP1I GCGC 2 cut(s) 157, 237
HindIII AAGCTT 2 cut(s) 866, 1362
HinfI GANTC 8 cut(s) 58, 229, 318, 409, 520, 1665, 2011, 2162
HpaII CCGG 3 cut(s) 176, 574, 1618
HphI GGTGA 3 cut(s) 1078, 1472, 2172
Hpy166II GTNNAC 4 cut(s) 373, 561, 1580, 1853
Hpy188I TCNGA 9 cut(s) 18, 253, 669, 907, 1275, 1464, 1935, 2049, 2167
Hpy188III TCNNGA 7 cut(s) 55, 79, 268, 695, 833, 1034, 1786
Hpy8I GTNNAC 4 cut(s) 373, 561, 1580, 1853
Hpy99I CGWCG 3 cut(s) 28, 154, 287
HpyAV CCTTC 6 cut(s) 202, 225, 595, 1577, 1683, 2193
HpyCH4III ACNGT 9 cut(s) 199, 380, 565, 903, 1221, 1545, 1686, 1837, 1857
HpyCH4IV ACGT 1 cut(s) 2154
HpyF3I CTNAG 3 cut(s) 129, 636, 990
HpySE526I ACGT 1 cut(s) 2154
Hsp92I GRCGYC 2 cut(s) 285, 806
HspAI GCGC 2 cut(s) 157, 237
Ksp22I TGATCA 2 cut(s) 553, 595
Kzo9I GATC 7 cut(s) 261, 439, 553, 595, 602, 1003, 1563
LguI GCTCTTC 2 cut(s) 311, 867
LmnI GCTCC 2 cut(s) 1036, 1410
Lsp1109I GCAGC 7 cut(s) 157, 166, 826, 983, 1006, 1127, 1936
LweI GCATC 5 cut(s) 553, 649, 826, 1582, 1961
MabI ACCWGGT 1 cut(s) 1573
MaeI CTAG 6 cut(s) 416, 569, 977, 1100, 1325, 1433
MaeII ACGT 1 cut(s) 2154
MaeIII GTNAC 7 cut(s) 533, 897, 930, 946, 1150, 1478, 1519
MalI GATC 7 cut(s) 263, 441, 555, 597, 604, 1005, 1565
MboI GATC 7 cut(s) 261, 439, 553, 595, 602, 1003, 1563
MflI RGATCY 5 cut(s) 261, 439, 602, 1003, 1563
MlsI TGGCCA 3 cut(s) 50, 384, 1864
MluNI TGGCCA 3 cut(s) 50, 384, 1864
MlyI GAGTC 1 cut(s) 418
MmeI TCCRAC 3 cut(s) 471, 1372, 1392
Mox20I TGGCCA 3 cut(s) 50, 384, 1864
MroXI GAANNNNTTC 2 cut(s) 876, 1280
MscI TGGCCA 3 cut(s) 50, 384, 1864
MseI TTAA 6 cut(s) 918, 1235, 1727, 1995, 2100, 2209
MslI CAYNNNNRTG 3 cut(s) 648, 834, 2109
Msp20I TGGCCA 3 cut(s) 50, 384, 1864
MspA1I CMGCKG 1 cut(s) 1949
MspI CCGG 3 cut(s) 176, 574, 1618
Mva1269I GAATGC 1 cut(s) 2056
MvaI CCWGG 9 cut(s) 47, 226, 386, 468, 710, 1163, 1230, 1476, 1575
MvnI CGCG 3 cut(s) 237, 246, 609
NciI CCSGG 3 cut(s) 176, 575, 1619
NcoI CCATGG 3 cut(s) 756, 780, 1209
NdeI CATATG 2 cut(s) 649, 1612
NdeII GATC 7 cut(s) 261, 439, 553, 595, 602, 1003, 1563
NlaIV GGNNCC 1 cut(s) 1032
NmuCI GTSAC 3 cut(s) 1150, 1478, 1519
NspI RCATGY 2 cut(s) 1001, 1851
PaeI GCATGC 1 cut(s) 1001
PaeR7I CTCGAG 1 cut(s) 266
PceI AGGCCT 1 cut(s) 45
PciI ACATGT 1 cut(s) 1847
PciSI GCTCTTC 2 cut(s) 311, 867
PctI GAATGC 1 cut(s) 2056
PdmI GAANNNNTTC 2 cut(s) 876, 1280
PfeI GAWTC 7 cut(s) 58, 229, 318, 520, 1665, 2011, 2162
Pfl23II CGTACG 1 cut(s) 96
PflMI CCANNNNNTGG 1 cut(s) 436
PfoI TCCNGGA 3 cut(s) 174, 224, 573
PleI GAGTC 1 cut(s) 417
PpsI GAGTC 1 cut(s) 417
PscI ACATGT 1 cut(s) 1847
Psp6I CCWGG 9 cut(s) 45, 224, 384, 466, 708, 1161, 1228, 1474, 1573
PspEI GGTNACC 1 cut(s) 1478
PspFI CCCAGC 1 cut(s) 703
PspGI CCWGG 9 cut(s) 45, 224, 384, 466, 708, 1161, 1228, 1474, 1573
PspLI CGTACG 1 cut(s) 96
PspN4I GGNNCC 1 cut(s) 1032
PspPI GGNCC 2 cut(s) 706, 753
PstNI CAGNNNCTG 1 cut(s) 1154
PsuI RGATCY 5 cut(s) 261, 439, 602, 1003, 1563
PvuII CAGCTG 1 cut(s) 1949
RsaI GTAC 5 cut(s) 98, 360, 677, 1609, 1859
RsaNI GTAC 5 cut(s) 97, 359, 676, 1608, 1858
RseI CAYNNNNRTG 3 cut(s) 648, 834, 2109
SapI GCTCTTC 2 cut(s) 311, 867
SaqAI TTAA 6 cut(s) 918, 1235, 1727, 1995, 2100, 2209
Sau3AI GATC 7 cut(s) 261, 439, 553, 595, 602, 1003, 1563
Sau96I GGNCC 2 cut(s) 706, 753
ScaI AGTACT 2 cut(s) 360, 1859
SchI GAGTC 1 cut(s) 418
SexAI ACCWGGT 1 cut(s) 1573
SfaNI GCATC 5 cut(s) 553, 649, 826, 1582, 1961
SfcI CTRYAG 1 cut(s) 1660
Sfr274I CTCGAG 1 cut(s) 266
SinI GGWCC 1 cut(s) 753
SlaI CTCGAG 1 cut(s) 266
SmiMI CAYNNNNRTG 3 cut(s) 648, 834, 2109
SmlI CTYRAG 3 cut(s) 266, 742, 1503
SmoI CTYRAG 3 cut(s) 266, 742, 1503
SpeI ACTAGT 1 cut(s) 976
SphI GCATGC 1 cut(s) 1001
SseBI AGGCCT 1 cut(s) 45
SsiI CCGC 9 cut(s) 29, 121, 163, 202, 205, 235, 244, 609, 751
SspI AATATT 1 cut(s) 2003
SspMI CTAG 6 cut(s) 416, 569, 977, 1100, 1325, 1433
StuI AGGCCT 1 cut(s) 45
StyI CCWWGG 5 cut(s) 435, 756, 780, 1209, 2055
TaaI ACNGT 9 cut(s) 199, 380, 565, 903, 1221, 1545, 1686, 1837, 1857
TaiI ACGT 1 cut(s) 2157
TaqI TCGA 4 cut(s) 183, 267, 282, 2204
TaqII GACCGA 1 cut(s) 1919
TatI WGTACW 3 cut(s) 358, 1607, 1857
TauI GCSGC 4 cut(s) 32, 123, 207, 237
TfiI GAWTC 7 cut(s) 58, 229, 318, 520, 1665, 2011, 2162
Tru1I TTAA 6 cut(s) 918, 1235, 1727, 1995, 2100, 2209
Tru9I TTAA 6 cut(s) 918, 1235, 1727, 1995, 2100, 2209
TscAI CASTG 5 cut(s) 202, 280, 568, 1159, 1689
TseFI GTSAC 3 cut(s) 1150, 1478, 1519
TseI GCWGC 7 cut(s) 145, 154, 839, 971, 994, 1115, 1949
Tsp45I GTSAC 3 cut(s) 1150, 1478, 1519
TspDTI ATGAA 9 cut(s) 17, 201, 869, 1273, 1452, 1845, 2003, 2013, 2082
TspRI CASTG 5 cut(s) 202, 280, 568, 1159, 1689
Van91I CCANNNNNTGG 1 cut(s) 436
VpaK11BI GGWCC 1 cut(s) 753
XapI RAATTY 5 cut(s) 323, 579, 626, 689, 2060
XceI RCATGY 2 cut(s) 1001, 1851
XhoI CTCGAG 1 cut(s) 266
XmnI GAANNNNTTC 2 cut(s) 876, 1280
XspI CTAG 6 cut(s) 416, 569, 977, 1100, 1325, 1433
ZrmI AGTACT 2 cut(s) 360, 1859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.