AT3G57550

guanylate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004677
Physical Location & Seq
Forward (+)
21306609 .. 21309570
2962 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G57550.1

Sequence Viewer

Length: 1170 bp
ATGGGAGAAGCACCAGCTTTCTTTGTGGATCATCTGGAGAATGGATACACCAATGGCTTTGGTGTCAAATCCGAGCCTAAGAACAGAGATACATCTGTGCTTATTGGGGATCGATCCTTTGTGATTGGTGGAAATCATGAAGGAAACCCATTGTTCCTTGGTGTTCAGATTCATGACAAAGTCACTAACAAGTGGTCCAGTCCTAGTGTTCTTGGAACTGGCCCTAAACCCTGCAAGGGTTACTCTGCCATTGTTCTTCCAAAAGGTCGGATTTTGGTTATCAAAAAAGGCTCAGCTTCTGATGACTCCATTTGGTTCCTTGAGGTGGATACTCCTTTTATCCGGGAACAAAAGAAATTGCTTGGAAGAGAGGTTGTTGCTTGGAGTAAAGGAGTAAGGGGAAATGCAGAGAAGCCTATTGTTATAAGCGGTCCTTCTGGAGTTGGAAAAGGAACACTTATATCGATGCTCATGAAGGAGTTTCCTTCAATGTTTGGGTTCTCTGTGAGCCACACGACTCGAGCTCCAAGGTGTATGGAGAAGAATGGTGTTCACTACCATTTCACTGATAAGACTGTTATGGAGAAAGAAATCAAAGATGGGAAGTTTCTCGAGTTTGCGTCTGTTCACGGCAATCTATACGGAACCAGCATTGAATCCGTTGAGGTTGTAACAGATTCAGGAAAGAGATGCATTTTGGATATTGATGTTCAAGGAGCGAGGTCAGTGAAGGCGAGTTCACTGGATGCGATATTCATATTCGTTTGTCCTCCTTCAATGAAGGAACTTGAAGATCGGCTTCGTGCCCGAGGAACAGAGACAGAGGAGCAAATCCAAAAGCGGCTTAGAAACGCTGACGCAGAGATCAAAGCAGGGAAATCCTCTGGCATTTTTGAACACAAACTGTATAATGACAACCTTGAGGAATGCTACAAGACCCTTAAGAATCTCTTGGGGATTAACGACCTTGCTCCTGTGAATGGTGTAGAAGTAGAAGGGATCAATCTGCCCAAGGAGCACACAGTAACAAAGATGGATGACAAGATTTTGATTCAAGAAACAGGAGAAGGAACCAAGAACAAAATGATCGTGTTGGATTTATCTTCAATCAACGGGGGAGCACCGGGAAGAACAAGAGGGATCGTTCTGGACACGGTTAAGTCCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004385 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006163 GO:0006164 GO:0006185 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009126 GO:0009132 GO:0009133 GO:0009135 GO:0009136 GO:0009141 GO:0009144 GO:0009150 GO:0009151 GO:0009152 GO:0009153 GO:0009161 GO:0009162 GO:0009163 GO:0009165 GO:0009167 GO:0009170 GO:0009179 GO:0009180 GO:0009182 GO:0009183 GO:0009185 GO:0009186 GO:0009188 GO:0009189 GO:0009199 GO:0009200 GO:0009205 GO:0009215 GO:0009225 GO:0009259 GO:0009260 GO:0009262 GO:0009263 GO:0009265 GO:0009394 GO:0009987 GO:0015031 GO:0015833 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016776 GO:0017144 GO:0018130 GO:0019205 GO:0019438 GO:0019637 GO:0019673 GO:0019692 GO:0019693 GO:0032501 GO:0032502 GO:0033036 GO:0034404 GO:0034436 GO:0034641 GO:0034654 GO:0040008 GO:0042278 GO:0042451 GO:0042455 GO:0042886 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046034 GO:0046037 GO:0046054 GO:0046060 GO:0046066 GO:0046128 GO:0046129 GO:0046385 GO:0046390 GO:0046483 GO:0046710 GO:0046711 GO:0046939 GO:0046940 GO:0048229 GO:0048638 GO:0048856 GO:0050145 GO:0050789 GO:0050793 GO:0051179 GO:0051234 GO:0055086 GO:0065007 GO:0071702 GO:0071704 GO:0071705 GO:0072521 GO:0072522 GO:0090407 GO:1901068 GO:1901070 GO:1901135 GO:1901137 GO:1901264 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659
KEGG Pathways
Metabolic & Signaling
ko00230 ko01100 map00230 map01100
Pfam Domains
Protein Families
Guanylate_kin

Protein Analysis

389

Amino Acids

42.55

Weight (kDa)

8.13

Isoelectric Point (pI)

25.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 24 - 98 5.5e-06 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 26 - 110 4.5e-06 FBX42, beta-propeller domain
Guanylate_kin PF00625 137 - 318 2e-56 Guanylate kinase
AAA_18 PF13238 140 - 289 9.7e-06 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 425
AciI CCGC 2 cut(s) 429, 841
AclWI GGATC 5 cut(s) 36, 108, 117, 1007, 1148
AfiI CCNNNNNNNGG 3 cut(s) 236, 325, 980
AflII CTTAAG 1 cut(s) 941
AgsI TTSAA 8 cut(s) 489, 656, 713, 777, 791, 896, 1055, 1107
AluBI AGCT 3 cut(s) 17, 296, 524
AluI AGCT 3 cut(s) 17, 296, 524
Alw21I GWGCWC 3 cut(s) 526, 1020, 1123
Alw26I GTCTC 1 cut(s) 812
AlwI GGATC 5 cut(s) 36, 108, 117, 1007, 1148
AlwNI CAGNNNCTG 1 cut(s) 299
Ama87I CYCGRG 3 cut(s) 519, 611, 807
AoxI GGCC 1 cut(s) 220
AspS9I GGNCC 3 cut(s) 195, 221, 431
AsuC2I CCSGG 2 cut(s) 344, 1125
AvaI CYCGRG 3 cut(s) 519, 611, 807
AvaII GGWCC 2 cut(s) 195, 431
BaeGI GKGCMC 1 cut(s) 808
BaeI ACNNNNGTAYC 2 cut(s) 81, 114
BanII GRGCYC 1 cut(s) 526
Bbv12I GWGCWC 3 cut(s) 526, 1020, 1123
BccI CCATC 2 cut(s) 593, 1027
BceAI ACGGC 1 cut(s) 646
BciVI GTATCC 2 cut(s) 38, 322
BcnI CCSGG 2 cut(s) 344, 1125
BcoDI GTCTC 1 cut(s) 812
BfaI CTAG 1 cut(s) 204
BfrI CTTAAG 1 cut(s) 941
BfuI GTATCC 2 cut(s) 38, 322
BisI GCNGC 1 cut(s) 842
BlpI GCTNAGC 1 cut(s) 292
BlsI GCNGC 1 cut(s) 843
Bme1390I CCNGG 2 cut(s) 344, 1125
Bme18I GGWCC 2 cut(s) 195, 431
BmeT110I CYCGRG 3 cut(s) 519, 611, 807
BmgT120I GGNCC 3 cut(s) 195, 221, 431
BmiI GGNNCC 3 cut(s) 317, 646, 1072
BmrFI CCNGG 2 cut(s) 344, 1125
BmsI GCATC 3 cut(s) 456, 680, 736
BpmI CTGGAG 2 cut(s) 56, 459
Bpu1102I GCTNAGC 1 cut(s) 292
BpuEI CTTGAG 2 cut(s) 341, 941
BpuMI CCSGG 2 cut(s) 344, 1125
Bsa29I ATCGAT 2 cut(s) 112, 464
BsaJI CCNNGG 4 cut(s) 157, 527, 808, 1011
BsaXI ACNNNNNCTCC 2 cut(s) 508, 538
Bsc4I CCNNNNNNNGG 3 cut(s) 236, 325, 980
Bse1I ACTGG 4 cut(s) 198, 223, 747, 1164
BseCI ATCGAT 2 cut(s) 112, 464
BseDI CCNNGG 4 cut(s) 157, 527, 808, 1011
BseGI GGATG 2 cut(s) 751, 1042
BseLI CCNNNNNNNGG 3 cut(s) 236, 325, 980
BseMII CTCAG 1 cut(s) 306
BseNI ACTGG 4 cut(s) 198, 223, 747, 1164
BseRI GAGGAG 1 cut(s) 839
BseSI GKGCMC 1 cut(s) 808
BshFI GGCC 1 cut(s) 222
BshVI ATCGAT 2 cut(s) 112, 464
BsiHKAI GWGCWC 3 cut(s) 526, 1020, 1123
BsiHKCI CYCGRG 3 cut(s) 519, 611, 807
BsiSI CCGG 2 cut(s) 343, 1124
BslI CCNNNNNNNGG 3 cut(s) 236, 325, 980
BsmAI GTCTC 1 cut(s) 812
BsmI GAATGC 1 cut(s) 932
BsnI GGCC 1 cut(s) 222
BsoBI CYCGRG 3 cut(s) 519, 611, 807
Bsp1286I GDGCHC 4 cut(s) 526, 808, 1020, 1123
Bsp143I GATC 8 cut(s) 28, 109, 113, 793, 864, 999, 1086, 1140
Bsp1720I GCTNAGC 1 cut(s) 292
BspACI CCGC 2 cut(s) 429, 841
BspANI GGCC 1 cut(s) 222
BspCNI CTCAG 1 cut(s) 305
BspDI ATCGAT 2 cut(s) 112, 464
BspHI TCATGA 3 cut(s) 136, 172, 471
BspLI GGNNCC 3 cut(s) 317, 646, 1072
BspPI GGATC 5 cut(s) 36, 108, 117, 1007, 1148
BspTI CTTAAG 1 cut(s) 941
BsrI ACTGG 4 cut(s) 198, 223, 747, 1164
BssECI CCNNGG 4 cut(s) 157, 527, 808, 1011
BssMI GATC 8 cut(s) 28, 109, 113, 793, 864, 999, 1086, 1140
BssT1I CCWWGG 3 cut(s) 157, 527, 1011
Bst4CI ACNGT 4 cut(s) 577, 906, 1024, 1156
Bst6I CTCTTC 1 cut(s) 361
BstAFI CTTAAG 1 cut(s) 941
BstDEI CTNAG 3 cut(s) 78, 292, 845
BstF5I GGATG 2 cut(s) 751, 1042
BstKTI GATC 8 cut(s) 31, 112, 116, 796, 867, 1002, 1089, 1143
BstMAI GTCTC 1 cut(s) 812
BstMBI GATC 8 cut(s) 28, 109, 113, 793, 864, 999, 1086, 1140
BstMWI GCNNNNNNNGC 1 cut(s) 1015
BstSCI CCNGG 2 cut(s) 342, 1123
BstSLI GKGCMC 1 cut(s) 808
Bsu15I ATCGAT 2 cut(s) 112, 464
BsuI GTATCC 2 cut(s) 38, 322
BsuRI GGCC 1 cut(s) 222
BsuTUI ATCGAT 2 cut(s) 112, 464
BtsCI GGATG 2 cut(s) 751, 1042
BtsIMutI CAGTG 3 cut(s) 564, 732, 740
CaiI CAGNNNCTG 1 cut(s) 299
CciI TCATGA 3 cut(s) 136, 172, 471
Cfr13I GGNCC 3 cut(s) 195, 221, 431
ClaI ATCGAT 2 cut(s) 112, 464
CseI GACGC 2 cut(s) 609, 866
CviAII CATG 3 cut(s) 137, 173, 472
DdeI CTNAG 3 cut(s) 78, 292, 845
DpnI GATC 8 cut(s) 30, 111, 115, 795, 866, 1001, 1088, 1142
DpnII GATC 8 cut(s) 28, 109, 113, 793, 864, 999, 1086, 1140
Eam1104I CTCTTC 1 cut(s) 361
EarI CTCTTC 1 cut(s) 361
Ecl136II GAGCTC 1 cut(s) 524
Eco130I CCWWGG 3 cut(s) 157, 527, 1011
Eco24I GRGCYC 1 cut(s) 526
Eco47I GGWCC 2 cut(s) 195, 431
Eco53kI GAGCTC 1 cut(s) 524
Eco88I CYCGRG 3 cut(s) 519, 611, 807
EcoICRI GAGCTC 1 cut(s) 524
EcoT14I CCWWGG 3 cut(s) 157, 527, 1011
EcoT22I ATGCAT 1 cut(s) 695
EcoT38I GRGCYC 1 cut(s) 526
ErhI CCWWGG 3 cut(s) 157, 527, 1011
FaeI CATG 3 cut(s) 140, 176, 475
FalI AAGNNNNNCTT 8 cut(s) 418, 450, 441, 473, 783, 815, 935, 967
FatI CATG 3 cut(s) 136, 172, 471
Fnu4HI GCNGC 1 cut(s) 842
FokI GGATG 2 cut(s) 758, 1049
FriOI GRGCYC 1 cut(s) 526
Fsp4HI GCNGC 1 cut(s) 842
FspBI CTAG 1 cut(s) 204
GluI GCNGC 1 cut(s) 842
GsuI CTGGAG 2 cut(s) 56, 459
HaeIII GGCC 1 cut(s) 222
HapII CCGG 2 cut(s) 343, 1124
HgaI GACGC 2 cut(s) 609, 866
Hin1II CATG 3 cut(s) 140, 176, 475
HinfI GANTC 7 cut(s) 169, 305, 517, 656, 677, 946, 1051
HpaII CCGG 2 cut(s) 343, 1124
Hpy166II GTNNAC 3 cut(s) 553, 628, 740
Hpy188I TCNGA 4 cut(s) 73, 168, 270, 301
Hpy188III TCNNGA 9 cut(s) 35, 137, 173, 438, 472, 611, 681, 1055, 1148
Hpy8I GTNNAC 3 cut(s) 553, 628, 740
HpyAV CCTTC 9 cut(s) 134, 444, 469, 495, 724, 775, 783, 989, 1061
HpyCH4III ACNGT 4 cut(s) 577, 906, 1024, 1156
HpyCH4V TGCA 3 cut(s) 234, 407, 693
HpyF10VI GCNNNNNNNGC 1 cut(s) 1015
HpyF3I CTNAG 3 cut(s) 78, 292, 845
Hsp92II CATG 3 cut(s) 140, 176, 475
Kzo9I GATC 8 cut(s) 28, 109, 113, 793, 864, 999, 1086, 1140
LmnI GCTCC 6 cut(s) 529, 716, 826, 976, 1015, 1118
LweI GCATC 3 cut(s) 456, 680, 736
MaeI CTAG 1 cut(s) 204
MaeIII GTNAC 4 cut(s) 181, 239, 670, 1024
MalI GATC 8 cut(s) 30, 111, 115, 795, 866, 1001, 1088, 1142
MboI GATC 8 cut(s) 28, 109, 113, 793, 864, 999, 1086, 1140
MboII GAAGA 6 cut(s) 248, 378, 553, 803, 1095, 1140
MhlI GDGCHC 4 cut(s) 526, 808, 1020, 1123
MluCI AATT 1 cut(s) 356
MlyI GAGTC 2 cut(s) 299, 511
MmeI TCCRAC 3 cut(s) 248, 424, 1074
Mph1103I ATGCAT 1 cut(s) 695
MseI TTAA 3 cut(s) 942, 960, 1158
MspCI CTTAAG 1 cut(s) 941
MspI CCGG 2 cut(s) 343, 1124
MspR9I CCNGG 2 cut(s) 344, 1125
Mva1269I GAATGC 1 cut(s) 932
MwoI GCNNNNNNNGC 1 cut(s) 1015
NciI CCSGG 2 cut(s) 344, 1125
NdeII GATC 8 cut(s) 28, 109, 113, 793, 864, 999, 1086, 1140
NlaIII CATG 3 cut(s) 140, 176, 475
NlaIV GGNNCC 3 cut(s) 317, 646, 1072
NmuCI GTSAC 1 cut(s) 181
NsiI ATGCAT 1 cut(s) 695
PaeR7I CTCGAG 2 cut(s) 519, 611
PagI TCATGA 3 cut(s) 136, 172, 471
PctI GAATGC 1 cut(s) 932
PfeI GAWTC 5 cut(s) 169, 656, 677, 946, 1051
PflFI GACNNNGTC 1 cut(s) 179
PfoI TCCNGGA 1 cut(s) 342
PkrI GCNGC 1 cut(s) 843
PleI GAGTC 2 cut(s) 299, 511
PpsI GAGTC 2 cut(s) 299, 511
PsiI TTATAA 1 cut(s) 425
Psp124BI GAGCTC 1 cut(s) 526
PspN4I GGNNCC 3 cut(s) 317, 646, 1072
PspPI GGNCC 3 cut(s) 195, 221, 431
PspXI VCTCGAGB 1 cut(s) 519
PstNI CAGNNNCTG 1 cut(s) 299
PsyI GACNNNGTC 1 cut(s) 179
SacI GAGCTC 1 cut(s) 526
SaqAI TTAA 3 cut(s) 942, 960, 1158
SatI GCNGC 1 cut(s) 842
Sau3AI GATC 8 cut(s) 28, 109, 113, 793, 864, 999, 1086, 1140
Sau96I GGNCC 3 cut(s) 195, 221, 431
SchI GAGTC 2 cut(s) 299, 511
ScrFI CCNGG 2 cut(s) 344, 1125
SduI GDGCHC 4 cut(s) 526, 808, 1020, 1123
SfaNI GCATC 3 cut(s) 456, 680, 736
Sfr274I CTCGAG 2 cut(s) 519, 611
SinI GGWCC 2 cut(s) 195, 431
SlaI CTCGAG 2 cut(s) 519, 611
SmlI CTYRAG 5 cut(s) 320, 519, 611, 920, 941
SmoI CTYRAG 5 cut(s) 320, 519, 611, 920, 941
Sse9I AATT 1 cut(s) 356
SsiI CCGC 2 cut(s) 429, 841
SspMI CTAG 1 cut(s) 204
SstI GAGCTC 1 cut(s) 526
StyD4I CCNGG 2 cut(s) 342, 1123
StyI CCWWGG 3 cut(s) 157, 527, 1011
TaaI ACNGT 4 cut(s) 577, 906, 1024, 1156
TaqI TCGA 4 cut(s) 112, 464, 520, 612
TasI AATT 1 cut(s) 356
TauI GCSGC 1 cut(s) 844
TfiI GAWTC 5 cut(s) 169, 656, 677, 946, 1051
Tru1I TTAA 3 cut(s) 942, 960, 1158
Tru9I TTAA 3 cut(s) 942, 960, 1158
TscAI CASTG 3 cut(s) 571, 732, 747
TseFI GTSAC 1 cut(s) 181
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 5 cut(s) 153, 161, 488, 745, 794
TspGWI ACGGA 2 cut(s) 649, 657
TspRI CASTG 3 cut(s) 571, 732, 747
Tth111I GACNNNGTC 1 cut(s) 179
Vha464I CTTAAG 1 cut(s) 941
VpaK11BI GGWCC 2 cut(s) 195, 431
XhoI CTCGAG 2 cut(s) 519, 611
XspI CTAG 1 cut(s) 204
Zsp2I ATGCAT 1 cut(s) 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.