MD14G1069300.v1.1

Guanylate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004677
Physical Location & Seq
Reverse (-)
7575236 .. 7579015
3780 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1069300.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGGGTGAAGCACCAGCATTCTTTGTTGATGAGCTGCAGAAGGGATTTGCAAATGGTTTCGATGTAAAATCAAAAGGCGGTGAAACTTCCGTTGCCTACTGCAACAGAACATATGTGATTGGTGGAGCCTGTGAGGAATCGGCATTGTCAATTGGAGTTCGTATTCTAGATCAATCCAGTGGTGAATGGATAAATCCTACTGTGTTGGGAACAAAACCCAAGCCATCGAAAGGCCATTCAGCAGTGCTTTTAGGTGAAGACCGAATATTGATTACCAAGAATGGTTCCAGCGCAGACGATTTTGCTTGGTTTCTTGAGGTGGACACCCAATATGTTAGGCAGCAGAAGAAAATTTTGGGCACCGAGGTTGTTGCGTGGAGTAAGGGTGTGAGTGGCTGTTCTGAGAAGCCAGTTGTTATTAGTGGTCCTTCCGGGGTAGGTAAGGGGACACTAATATCCATGCTCATGAAAGAATATCCATCCATGTTTGGATTCTCTGTGAGCCACACAACCCGTGCTGCAAGAGCTATGGAAAAGGATGGGGTCCATTACCATTTCATTGAGAGGAGTATCATGGAGAAAGAATTAGAAGATGGCAAGTTCCTTGAGTTTGCTTCTGTGCATGGAAATCTCTACGGAACTAGTGTTGAGGCAGTAGAACTCGTAGCAGATGATGGGAAGAGATGCATTCTTGACATCGATGTTCAAGGGGCAAGATCCGTGAGAGCCAGTTCACTTGAAGCTATCTTCATCTTCGTATGTCCACCCTCAATGGAAGAGCTTGAGAAGCGCCTTCGTGCAAGGGGAACTGAGACAGAGGAACAGGTCCTAAAGCGACTTCGAAATGCCAAGACAGAGATTGATCTAGGGCAGTCATCAGGCATCTTTGATCATATGTTGTATAATGATAACCTGGAAGACTGTTACCAGAGTCTAAAGGAACTCTTGGGGCTAGATGGAACTGTCACTGCAGCTCCTAAATCAGCAACTAAAGTGGTTGATCTGCCAATGGACCATTCGTTGACCAAGATTGATAACAAAATCATCATAAACTCCAGAACTCCAGAACTAGAAAAAGCATCGACGAACATGATCGTGCTGGATGTGTCATCCCTCAAAGGAGGGGCGCCTGGACGGACTAGAGGACTAGACATTCATACCATGGACTCATTTCTAGATGGTCTGTCCGGGATTACTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004385 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006163 GO:0006164 GO:0006185 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009126 GO:0009132 GO:0009133 GO:0009135 GO:0009136 GO:0009141 GO:0009144 GO:0009150 GO:0009151 GO:0009152 GO:0009153 GO:0009161 GO:0009162 GO:0009163 GO:0009165 GO:0009167 GO:0009170 GO:0009179 GO:0009180 GO:0009182 GO:0009183 GO:0009185 GO:0009186 GO:0009188 GO:0009189 GO:0009199 GO:0009200 GO:0009205 GO:0009215 GO:0009225 GO:0009259 GO:0009260 GO:0009262 GO:0009263 GO:0009265 GO:0009394 GO:0009987 GO:0015031 GO:0015833 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016776 GO:0017144 GO:0018130 GO:0019205 GO:0019438 GO:0019637 GO:0019673 GO:0019692 GO:0019693 GO:0032501 GO:0032502 GO:0033036 GO:0034404 GO:0034436 GO:0034641 GO:0034654 GO:0040008 GO:0042278 GO:0042451 GO:0042455 GO:0042886 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046034 GO:0046037 GO:0046054 GO:0046060 GO:0046066 GO:0046128 GO:0046129 GO:0046385 GO:0046390 GO:0046483 GO:0046710 GO:0046711 GO:0046939 GO:0046940 GO:0048229 GO:0048638 GO:0048856 GO:0050145 GO:0050789 GO:0050793 GO:0051179 GO:0051234 GO:0055086 GO:0065007 GO:0071702 GO:0071704 GO:0071705 GO:0072521 GO:0072522 GO:0090407 GO:1901068 GO:1901070 GO:1901135 GO:1901137 GO:1901264 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659
KEGG Pathways
Metabolic & Signaling
ko00230 ko01100 map00230 map01100
Pfam Domains
Protein Families
Guanylate_kin

Protein Analysis

401

Amino Acids

43.54

Weight (kDa)

5.43

Isoelectric Point (pI)

32.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_FBX42 PF13415 26 - 97 7.7e-09 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 27 - 91 1.3e-06 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Guanylate_kin PF00625 135 - 316 3.1e-57 Guanylate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 359, 1126
AciI CCGC 1 cut(s) 78
AclWI GGATC 1 cut(s) 711
AcsI RAATTY 1 cut(s) 351
AcyI GRCGYC 1 cut(s) 1127
AfiI CCNNNNNNNGG 1 cut(s) 230
AgsI TTSAA 2 cut(s) 707, 740
AhlI ACTAGT 1 cut(s) 641
AjnI CCWGG 2 cut(s) 912, 1129
AjuI GAANNNNNNNTTGG 4 cut(s) 338, 370, 1000, 1032
AluBI AGCT 5 cut(s) 34, 527, 743, 781, 974
AluI AGCT 5 cut(s) 34, 527, 743, 781, 974
Alw26I GTCTC 1 cut(s) 806
AlwI GGATC 1 cut(s) 711
AoxI GGCC 1 cut(s) 232
ApeKI GCWGC 4 cut(s) 34, 340, 518, 971
ApoI RAATTY 1 cut(s) 351
AspLEI GCGC 3 cut(s) 293, 792, 1129
AspS9I GGNCC 4 cut(s) 425, 544, 826, 1012
AsuC2I CCSGG 2 cut(s) 433, 1189
AsuHPI GGTGA 4 cut(s) 17, 92, 194, 266
AsuII TTCGAA 1 cut(s) 841
AvaII GGWCC 4 cut(s) 425, 544, 826, 1012
BaeGI GKGCMC 1 cut(s) 362
BanI GGYRCC 2 cut(s) 359, 1126
BbsI GAAGAC 2 cut(s) 264, 924
BbvI GCAGC 4 cut(s) 21, 352, 505, 983
BccI CCATC 7 cut(s) 232, 487, 533, 587, 668, 950, 1172
BcgI CGANNNNNNTGC 2 cut(s) 353, 387
BciT130I CCWGG 2 cut(s) 914, 1131
BclI TGATCA 1 cut(s) 889
BcnI CCSGG 2 cut(s) 433, 1189
BcoDI GTCTC 1 cut(s) 806
BcuI ACTAGT 1 cut(s) 641
BfaI CTAG 8 cut(s) 167, 642, 866, 953, 1070, 1140, 1148, 1175
BfmI CTRYAG 2 cut(s) 35, 969
BfoI RGCGCY 2 cut(s) 793, 1130
BisI GCNGC 4 cut(s) 35, 341, 519, 972
BlsI GCNGC 4 cut(s) 36, 342, 520, 973
Bme1390I CCNGG 4 cut(s) 433, 914, 1131, 1189
Bme18I GGWCC 4 cut(s) 425, 544, 826, 1012
BmgT120I GGNCC 4 cut(s) 425, 544, 826, 1012
BmiI GGNNCC 5 cut(s) 127, 286, 361, 545, 1128
BmrFI CCNGG 4 cut(s) 433, 914, 1131, 1189
BmsI GCATC 3 cut(s) 674, 891, 1088
BpiI GAAGAC 2 cut(s) 264, 924
BpmI CTGGAG 2 cut(s) 1039, 1047
Bpu14I TTCGAA 1 cut(s) 841
BpuEI CTTGAG 3 cut(s) 335, 626, 803
BpuMI CCSGG 2 cut(s) 433, 1189
Bsa29I ATCGAT 1 cut(s) 699
BsaHI GRCGYC 1 cut(s) 1127
BsaJI CCNNGG 3 cut(s) 363, 432, 1161
BsaXI ACNNNNNCTCC 4 cut(s) 370, 400, 958, 988
Bsc4I CCNNNNNNNGG 1 cut(s) 230
Bse1I ACTGG 3 cut(s) 177, 410, 729
BseBI CCWGG 2 cut(s) 914, 1131
BseCI ATCGAT 1 cut(s) 699
BseDI CCNNGG 3 cut(s) 363, 432, 1161
BseGI GGATG 4 cut(s) 479, 544, 1108, 1109
BseLI CCNNNNNNNGG 1 cut(s) 230
BseMII CTCAG 2 cut(s) 393, 801
BseNI ACTGG 3 cut(s) 177, 410, 729
BseRI GAGGAG 1 cut(s) 580
BseSI GKGCMC 1 cut(s) 362
BseXI GCAGC 4 cut(s) 21, 352, 505, 983
BshFI GGCC 1 cut(s) 234
BshNI GGYRCC 2 cut(s) 359, 1126
BshVI ATCGAT 1 cut(s) 699
BsiSI CCGG 2 cut(s) 432, 1188
BslFI GGGAC 1 cut(s) 460
BslI CCNNNNNNNGG 1 cut(s) 230
BsmAI GTCTC 1 cut(s) 806
BsmFI GGGAC 1 cut(s) 460
BsmI GAATGC 2 cut(s) 17, 687
BsnI GGCC 1 cut(s) 234
Bsp119I TTCGAA 1 cut(s) 841
Bsp1286I GDGCHC 1 cut(s) 362
Bsp143I GATC 6 cut(s) 169, 716, 862, 889, 1000, 1092
Bsp19I CCATGG 1 cut(s) 1161
BspACI CCGC 1 cut(s) 78
BspANI GGCC 1 cut(s) 234
BspCNI CTCAG 2 cut(s) 394, 802
BspDI ATCGAT 1 cut(s) 699
BspHI TCATGA 1 cut(s) 465
BspLI GGNNCC 5 cut(s) 127, 286, 361, 545, 1128
BspMAI CTGCAG 2 cut(s) 39, 973
BspPI GGATC 1 cut(s) 711
BspQI GCTCTTC 1 cut(s) 771
BspT104I TTCGAA 1 cut(s) 841
BspT107I GGYRCC 2 cut(s) 359, 1126
BsrI ACTGG 3 cut(s) 177, 410, 729
BssECI CCNNGG 3 cut(s) 363, 432, 1161
BssMI GATC 6 cut(s) 169, 716, 862, 889, 1000, 1092
BssNI GRCGYC 1 cut(s) 1127
BssT1I CCWWGG 1 cut(s) 1161
Bst2UI CCWGG 2 cut(s) 914, 1131
Bst4CI ACNGT 3 cut(s) 202, 923, 964
Bst6I CTCTTC 2 cut(s) 674, 771
BstACI GRCGYC 1 cut(s) 1127
BstBI TTCGAA 1 cut(s) 841
BstDEI CTNAG 2 cut(s) 402, 810
BstDSI CCRYGG 1 cut(s) 1161
BstF5I GGATG 4 cut(s) 479, 544, 1108, 1109
BstH2I RGCGCY 2 cut(s) 793, 1130
BstHHI GCGC 3 cut(s) 293, 792, 1129
BstKTI GATC 6 cut(s) 172, 719, 865, 892, 1003, 1095
BstMAI GTCTC 1 cut(s) 806
BstMBI GATC 6 cut(s) 169, 716, 862, 889, 1000, 1092
BstMWI GCNNNNNNNGC 2 cut(s) 524, 787
BstNI CCWGG 2 cut(s) 914, 1131
BstSCI CCNGG 4 cut(s) 431, 912, 1129, 1187
BstSFI CTRYAG 2 cut(s) 35, 969
BstSLI GKGCMC 1 cut(s) 362
BstV1I GCAGC 4 cut(s) 21, 352, 505, 983
BstV2I GAAGAC 2 cut(s) 264, 924
BstX2I RGATCY 1 cut(s) 716
BstYI RGATCY 1 cut(s) 716
Bsu15I ATCGAT 1 cut(s) 699
BsuRI GGCC 1 cut(s) 234
BsuTUI ATCGAT 1 cut(s) 699
BtgI CCRYGG 1 cut(s) 1161
BtsCI GGATG 4 cut(s) 479, 544, 1108, 1109
BtsI GCAGTG 2 cut(s) 249, 966
BtsIMutI CAGTG 4 cut(s) 184, 249, 966, 1198
CciI TCATGA 1 cut(s) 465
CfoI GCGC 3 cut(s) 293, 792, 1129
Cfr13I GGNCC 4 cut(s) 425, 544, 826, 1012
ClaI ATCGAT 1 cut(s) 699
CspCI CAANNNNNGTGG 2 cut(s) 975, 1010
CviAII CATG 7 cut(s) 460, 466, 484, 574, 623, 1090, 1162
DdeI CTNAG 2 cut(s) 402, 810
DinI GGCGCC 1 cut(s) 1128
DpnI GATC 6 cut(s) 171, 718, 864, 891, 1002, 1094
DpnII GATC 6 cut(s) 169, 716, 862, 889, 1000, 1092
Eam1104I CTCTTC 2 cut(s) 674, 771
EarI CTCTTC 2 cut(s) 674, 771
Eco130I CCWWGG 1 cut(s) 1161
Eco47I GGWCC 4 cut(s) 425, 544, 826, 1012
EcoO109I RGGNCCY 1 cut(s) 826
EcoRII CCWGG 2 cut(s) 912, 1129
EcoT14I CCWWGG 1 cut(s) 1161
EcoT22I ATGCAT 1 cut(s) 689
EgeI GGCGCC 1 cut(s) 1128
EheI GGCGCC 1 cut(s) 1128
ErhI CCWWGG 1 cut(s) 1161
FaeI CATG 7 cut(s) 463, 469, 487, 577, 626, 1093, 1165
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FaqI GGGAC 1 cut(s) 460
FatI CATG 7 cut(s) 459, 465, 483, 573, 622, 1089, 1161
FauNDI CATATG 2 cut(s) 112, 894
FbaI TGATCA 1 cut(s) 889
Fnu4HI GCNGC 4 cut(s) 35, 341, 519, 972
FokI GGATG 4 cut(s) 466, 551, 1096, 1115
Fsp4HI GCNGC 4 cut(s) 35, 341, 519, 972
FspBI CTAG 8 cut(s) 167, 642, 866, 953, 1070, 1140, 1148, 1175
GlaI GCGC 3 cut(s) 292, 791, 1128
GluI GCNGC 4 cut(s) 35, 341, 519, 972
GsuI CTGGAG 2 cut(s) 1039, 1047
HaeII RGCGCY 2 cut(s) 793, 1130
HaeIII GGCC 1 cut(s) 234
HapII CCGG 2 cut(s) 432, 1188
HhaI GCGC 3 cut(s) 293, 792, 1129
Hin1I GRCGYC 1 cut(s) 1127
Hin1II CATG 7 cut(s) 463, 469, 487, 577, 626, 1093, 1165
Hin6I GCGC 3 cut(s) 291, 790, 1127
HinP1I GCGC 3 cut(s) 291, 790, 1127
HincII GTYRAC 1 cut(s) 1023
HindII GTYRAC 1 cut(s) 1023
HinfI GANTC 4 cut(s) 137, 492, 931, 1166
HpaII CCGG 2 cut(s) 432, 1188
HphI GGTGA 4 cut(s) 17, 92, 194, 266
Hpy166II GTNNAC 4 cut(s) 322, 734, 764, 1023
Hpy188I TCNGA 1 cut(s) 403
Hpy188III TCNNGA 7 cut(s) 167, 314, 466, 692, 1056, 1064, 1175
Hpy8I GTNNAC 4 cut(s) 322, 734, 764, 1023
Hpy99I CGWCG 1 cut(s) 1087
HpyAV CCTTC 3 cut(s) 34, 438, 803
HpyCH4III ACNGT 3 cut(s) 202, 923, 964
HpyCH4V TGCA 8 cut(s) 37, 50, 102, 521, 622, 687, 800, 971
HpyF10VI GCNNNNNNNGC 2 cut(s) 524, 787
HpyF3I CTNAG 2 cut(s) 402, 810
Hsp92I GRCGYC 1 cut(s) 1127
Hsp92II CATG 7 cut(s) 463, 469, 487, 577, 626, 1093, 1165
HspAI GCGC 3 cut(s) 291, 790, 1127
KasI GGCGCC 1 cut(s) 1126
Ksp22I TGATCA 1 cut(s) 889
Kzo9I GATC 6 cut(s) 169, 716, 862, 889, 1000, 1092
LguI GCTCTTC 1 cut(s) 771
LmnI GCTCC 2 cut(s) 125, 979
Lsp1109I GCAGC 4 cut(s) 21, 352, 505, 983
LweI GCATC 3 cut(s) 674, 891, 1088
MaeI CTAG 8 cut(s) 167, 642, 866, 953, 1070, 1140, 1148, 1175
MaeIII GTNAC 2 cut(s) 923, 964
MalI GATC 6 cut(s) 171, 718, 864, 891, 1002, 1094
MboI GATC 6 cut(s) 169, 716, 862, 889, 1000, 1092
MboII GAAGA 8 cut(s) 269, 358, 602, 691, 739, 745, 788, 929
MfeI CAATTG 1 cut(s) 150
MflI RGATCY 1 cut(s) 716
MhlI GDGCHC 1 cut(s) 362
MluCI AATT 3 cut(s) 150, 351, 584
Mly113I GGCGCC 1 cut(s) 1127
MlyI GAGTC 2 cut(s) 940, 1160
Mph1103I ATGCAT 1 cut(s) 689
MslI CAYNNNNRTG 2 cut(s) 464, 1094
MspI CCGG 2 cut(s) 432, 1188
MspR9I CCNGG 4 cut(s) 433, 914, 1131, 1189
MunI CAATTG 1 cut(s) 150
Mva1269I GAATGC 2 cut(s) 17, 687
MvaI CCWGG 2 cut(s) 914, 1131
MwoI GCNNNNNNNGC 2 cut(s) 524, 787
NarI GGCGCC 1 cut(s) 1127
NciI CCSGG 2 cut(s) 433, 1189
NcoI CCATGG 1 cut(s) 1161
NdeI CATATG 2 cut(s) 112, 894
NdeII GATC 6 cut(s) 169, 716, 862, 889, 1000, 1092
NlaIII CATG 7 cut(s) 463, 469, 487, 577, 626, 1093, 1165
NlaIV GGNNCC 5 cut(s) 127, 286, 361, 545, 1128
NmuCI GTSAC 1 cut(s) 964
NsiI ATGCAT 1 cut(s) 689
NspV TTCGAA 1 cut(s) 841
PagI TCATGA 1 cut(s) 465
PciSI GCTCTTC 1 cut(s) 771
PctI GAATGC 2 cut(s) 17, 687
PfeI GAWTC 2 cut(s) 137, 492
PfoI TCCNGGA 1 cut(s) 1187
PkrI GCNGC 4 cut(s) 36, 342, 520, 973
PleI GAGTC 2 cut(s) 939, 1160
PluTI GGCGCC 1 cut(s) 1130
PpsI GAGTC 2 cut(s) 939, 1160
PpuMI RGGWCCY 1 cut(s) 826
Psp5II RGGWCCY 1 cut(s) 826
Psp6I CCWGG 2 cut(s) 912, 1129
PspGI CCWGG 2 cut(s) 912, 1129
PspN4I GGNNCC 5 cut(s) 127, 286, 361, 545, 1128
PspPI GGNCC 4 cut(s) 425, 544, 826, 1012
PspPPI RGGWCCY 1 cut(s) 826
PstI CTGCAG 2 cut(s) 39, 973
PsuI RGATCY 1 cut(s) 716
RseI CAYNNNNRTG 2 cut(s) 464, 1094
SapI GCTCTTC 1 cut(s) 771
SatI GCNGC 4 cut(s) 35, 341, 519, 972
Sau3AI GATC 6 cut(s) 169, 716, 862, 889, 1000, 1092
Sau96I GGNCC 4 cut(s) 425, 544, 826, 1012
SchI GAGTC 2 cut(s) 940, 1160
ScrFI CCNGG 4 cut(s) 433, 914, 1131, 1189
SduI GDGCHC 1 cut(s) 362
SfaNI GCATC 3 cut(s) 674, 891, 1088
SfcI CTRYAG 2 cut(s) 35, 969
SfoI GGCGCC 1 cut(s) 1128
SfuI TTCGAA 1 cut(s) 841
SinI GGWCC 4 cut(s) 425, 544, 826, 1012
SmiMI CAYNNNNRTG 2 cut(s) 464, 1094
SmlI CTYRAG 3 cut(s) 314, 605, 782
SmoI CTYRAG 3 cut(s) 314, 605, 782
SpeI ACTAGT 1 cut(s) 641
Sse9I AATT 3 cut(s) 150, 351, 584
SsiI CCGC 1 cut(s) 78
SspDI GGCGCC 1 cut(s) 1126
SspI AATATT 1 cut(s) 267
SspMI CTAG 8 cut(s) 167, 642, 866, 953, 1070, 1140, 1148, 1175
StyD4I CCNGG 4 cut(s) 431, 912, 1129, 1187
StyI CCWWGG 1 cut(s) 1161
TaaI ACNGT 3 cut(s) 202, 923, 964
TaqI TCGA 5 cut(s) 60, 227, 699, 841, 1082
TaqII GACCGA 1 cut(s) 276
TasI AATT 3 cut(s) 150, 351, 584
TfiI GAWTC 2 cut(s) 137, 492
TscAI CASTG 3 cut(s) 184, 249, 973
TseFI GTSAC 1 cut(s) 964
TseI GCWGC 4 cut(s) 34, 340, 518, 971
Tsp45I GTSAC 1 cut(s) 964
TspDTI ATGAA 4 cut(s) 482, 547, 739, 1145
TspGWI ACGGA 4 cut(s) 79, 651, 709, 1150
TspRI CASTG 3 cut(s) 184, 249, 973
VpaK11BI GGWCC 4 cut(s) 425, 544, 826, 1012
XapI RAATTY 1 cut(s) 351
XbaI TCTAGA 2 cut(s) 166, 1174
XspI CTAG 8 cut(s) 167, 642, 866, 953, 1070, 1140, 1148, 1175
Zsp2I ATGCAT 1 cut(s) 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.