Rh3BG277900

Guanylate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0004677
Physical Location & Seq
Reverse (-)
27274951 .. 27279842
4892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG277900.1

Sequence Viewer

Length: 1035 bp
ATGGGAGAAGCCCCAGCATTCTTTGGTGATGAGCTTCAGGAGGGATATGCAAATGGGTTTGATCTGAAGTCCAAAGGCGGTGAAACCTCCGTTGACGTGGGCAACAGAACATATGTGATTGGTGGAGACTGTGAGGAATCAGCACTATCAATTGGAGTTCGGATTTTTGACAAAACTAGTGGTGAATGGATAAATCCTACTGTGTTGGGGACGCAACCCAAACCAGGTAAAGGCCATTCTGCAGTGCTTATGGGTGGAGATCGAATATTGATTATTAAGAGTGGTTCAAATTCAGATGATTTCGCCTGGTTTCTTGAGGTGGACACCCAATATGTTAGGCAGCAGAAGAAAATTTTAGGCACAGAGGTTGTTGCTTGGAGTAGGGGTGTGAGAGGCAGTGCTGAGAAGCCAGTAGTTATTAGTGGTCCTTCTGGGGTAGGTAAGGGGACACTAATATCAATGCTCACGAACGAATATCCATCCCTGTTTGGATTCTCTGTGAGCCACACGACTCGTGCTCCAAGAGCTATGGAGAAGGATGGGGTTCATTATCATTTCATCGAGCGAAGTGCAATGGAGAAAGAGATAGAAGATGGGAAATTCCTTGAGTTTGCTTCTGTGCACGGAAATCTCTATGGAACTAGTGTTGAAGCAGTAGAAGCGGTAGCAGATGCTGGAAAGATATGCATTCTTGACATTGATGTTCAAGGGGCAAGATCAGTGAGGGCTAGTTCGCTTGAAGCTCTCTTTATCTTTGTGTGTCCTCCCTCAATGGAGGAGCTTGAGAAGCGCCTTCGTGCAAGGGGAACCGAGACAGAGGAACAGGTCCTAAAGAGACTTCAAAATGCCAAGGCAGAGATTGAGCAGGGGCAATCCTCAGACATCTTTGATCATATGTTGTATAATGATAATCTTGAAGATAGTTACAAGAGTCTTAAGGAACTTTTGGGGCTGGATGAAAGTGTGGCTGCTCCTAAATCATGTAAGAGGTTCTTCAATTGTAACTGTTGTTTCTTTGGATTTTATCGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004385 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006163 GO:0006164 GO:0006185 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009126 GO:0009132 GO:0009133 GO:0009135 GO:0009136 GO:0009141 GO:0009144 GO:0009150 GO:0009151 GO:0009152 GO:0009153 GO:0009161 GO:0009162 GO:0009163 GO:0009165 GO:0009167 GO:0009170 GO:0009179 GO:0009180 GO:0009182 GO:0009183 GO:0009185 GO:0009186 GO:0009188 GO:0009189 GO:0009199 GO:0009200 GO:0009205 GO:0009215 GO:0009225 GO:0009259 GO:0009260 GO:0009262 GO:0009263 GO:0009265 GO:0009394 GO:0009987 GO:0015031 GO:0015833 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016776 GO:0017144 GO:0018130 GO:0019205 GO:0019438 GO:0019637 GO:0019673 GO:0019692 GO:0019693 GO:0032501 GO:0032502 GO:0033036 GO:0034404 GO:0034436 GO:0034641 GO:0034654 GO:0040008 GO:0042278 GO:0042451 GO:0042455 GO:0042886 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046034 GO:0046037 GO:0046054 GO:0046060 GO:0046066 GO:0046128 GO:0046129 GO:0046385 GO:0046390 GO:0046483 GO:0046710 GO:0046711 GO:0046939 GO:0046940 GO:0048229 GO:0048638 GO:0048856 GO:0050145 GO:0050789 GO:0050793 GO:0051179 GO:0051234 GO:0055086 GO:0065007 GO:0071702 GO:0071704 GO:0071705 GO:0072521 GO:0072522 GO:0090407 GO:1901068 GO:1901070 GO:1901135 GO:1901137 GO:1901264 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659
KEGG Pathways
Metabolic & Signaling
ko00230 ko01100 map00230 map01100
Pfam Domains
Protein Families
Guanylate_kin

Protein Analysis

344

Amino Acids

37.78

Weight (kDa)

5.14

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_FBX42 PF13415 27 - 108 1.2e-08 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 29 - 98 9.5e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Guanylate_kin PF00625 135 - 316 2.6e-60 Guanylate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 78, 662
AcsI RAATTY 3 cut(s) 289, 351, 599
AcuI CTGAAG 2 cut(s) 20, 86
AfiI CCNNNNNNNGG 2 cut(s) 224, 230
AflII CTTAAG 1 cut(s) 935
AgsI TTSAA 7 cut(s) 288, 650, 707, 740, 842, 917, 997
AhlI ACTAGT 2 cut(s) 176, 641
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 2 cut(s) 223, 305
AluBI AGCT 4 cut(s) 34, 527, 743, 781
AluI AGCT 4 cut(s) 34, 527, 743, 781
Alw21I GWGCWC 2 cut(s) 520, 624
Alw26I GTCTC 3 cut(s) 120, 806, 829
Alw44I GTGCAC 1 cut(s) 620
AlwNI CAGNNNCTG 1 cut(s) 674
AoxI GGCC 1 cut(s) 232
ApaLI GTGCAC 1 cut(s) 620
ApeKI GCWGC 2 cut(s) 340, 968
ApoI RAATTY 3 cut(s) 289, 351, 599
AspLEI GCGC 1 cut(s) 792
AspS9I GGNCC 2 cut(s) 425, 826
AsuHPI GGTGA 3 cut(s) 38, 92, 194
AvaII GGWCC 2 cut(s) 425, 826
BaeGI GKGCMC 1 cut(s) 624
BauI CACGAG 1 cut(s) 513
Bbv12I GWGCWC 2 cut(s) 520, 624
BbvI GCAGC 2 cut(s) 352, 955
BccI CCATC 3 cut(s) 487, 533, 587
BcgI CGANNNNNNTGC 2 cut(s) 551, 585
BciT130I CCWGG 2 cut(s) 225, 307
BclI TGATCA 1 cut(s) 889
BcoDI GTCTC 3 cut(s) 120, 806, 829
BcuI ACTAGT 2 cut(s) 176, 641
BfaI CTAG 3 cut(s) 177, 642, 729
BfmI CTRYAG 1 cut(s) 240
BfoI RGCGCY 1 cut(s) 793
BfrI CTTAAG 1 cut(s) 935
BisI GCNGC 2 cut(s) 341, 969
BlsI GCNGC 2 cut(s) 342, 970
Bme1390I CCNGG 2 cut(s) 225, 307
Bme18I GGWCC 2 cut(s) 425, 826
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 2 cut(s) 425, 826
BmiI GGNNCC 1 cut(s) 808
BmrFI CCNGG 2 cut(s) 225, 307
BmsI GCATC 1 cut(s) 661
BpuEI CTTGAG 3 cut(s) 335, 626, 803
BsaJI CCNNGG 1 cut(s) 849
BsaXI ACNNNNNCTCC 4 cut(s) 370, 400, 502, 532
Bsc4I CCNNNNNNNGG 2 cut(s) 224, 230
Bse1I ACTGG 1 cut(s) 410
Bse3DI GCAATG 1 cut(s) 579
BseBI CCWGG 2 cut(s) 225, 307
BseDI CCNNGG 1 cut(s) 849
BseGI GGATG 3 cut(s) 479, 544, 961
BseLI CCNNNNNNNGG 2 cut(s) 224, 230
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 2 cut(s) 393, 891
BseNI ACTGG 1 cut(s) 410
BseRI GAGGAG 1 cut(s) 791
BseSI GKGCMC 1 cut(s) 624
BseXI GCAGC 2 cut(s) 352, 955
BseYI CCCAGC 1 cut(s) 13
BshFI GGCC 1 cut(s) 234
BsiHKAI GWGCWC 2 cut(s) 520, 624
BslFI GGGAC 2 cut(s) 223, 460
BslI CCNNNNNNNGG 2 cut(s) 224, 230
BsmAI GTCTC 3 cut(s) 120, 806, 829
BsmFI GGGAC 2 cut(s) 223, 460
BsmI GAATGC 2 cut(s) 17, 687
BsnI GGCC 1 cut(s) 234
Bsp1286I GDGCHC 2 cut(s) 520, 624
Bsp143I GATC 4 cut(s) 61, 259, 716, 889
BspACI CCGC 2 cut(s) 78, 662
BspANI GGCC 1 cut(s) 234
BspCNI CTCAG 2 cut(s) 394, 890
BspLI GGNNCC 1 cut(s) 808
BspMAI CTGCAG 1 cut(s) 244
BspTI CTTAAG 1 cut(s) 935
BsrDI GCAATG 1 cut(s) 579
BsrI ACTGG 1 cut(s) 410
BssECI CCNNGG 1 cut(s) 849
BssMI GATC 4 cut(s) 61, 259, 716, 889
BssSI CACGAG 1 cut(s) 513
BssT1I CCWWGG 1 cut(s) 849
Bst2BI CACGAG 1 cut(s) 513
Bst2UI CCWGG 2 cut(s) 225, 307
Bst4CI ACNGT 3 cut(s) 131, 202, 1007
BstAFI CTTAAG 1 cut(s) 935
BstDEI CTNAG 2 cut(s) 402, 877
BstF5I GGATG 3 cut(s) 479, 544, 961
BstH2I RGCGCY 1 cut(s) 793
BstHHI GCGC 1 cut(s) 792
BstKTI GATC 4 cut(s) 64, 262, 719, 892
BstMAI GTCTC 3 cut(s) 120, 806, 829
BstMBI GATC 4 cut(s) 61, 259, 716, 889
BstMWI GCNNNNNNNGC 3 cut(s) 524, 659, 787
BstNI CCWGG 2 cut(s) 225, 307
BstSCI CCNGG 2 cut(s) 223, 305
BstSFI CTRYAG 1 cut(s) 240
BstSLI GKGCMC 1 cut(s) 624
BstV1I GCAGC 2 cut(s) 352, 955
BsuRI GGCC 1 cut(s) 234
BtrI CACGTC 1 cut(s) 97
BtsCI GGATG 3 cut(s) 479, 544, 961
BtsI GCAGTG 2 cut(s) 249, 403
BtsIMutI CAGTG 3 cut(s) 249, 403, 726
CaiI CAGNNNCTG 1 cut(s) 674
CfoI GCGC 1 cut(s) 792
Cfr13I GGNCC 2 cut(s) 425, 826
CseI GACGC 1 cut(s) 220
CsiI ACCWGGT 1 cut(s) 223
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviAII CATG 1 cut(s) 981
DdeI CTNAG 2 cut(s) 402, 877
DpnI GATC 4 cut(s) 63, 261, 718, 891
DpnII GATC 4 cut(s) 61, 259, 716, 889
Eco130I CCWWGG 1 cut(s) 849
Eco47I GGWCC 2 cut(s) 425, 826
Eco57I CTGAAG 2 cut(s) 20, 86
EcoO109I RGGNCCY 1 cut(s) 826
EcoRII CCWGG 2 cut(s) 223, 305
EcoT14I CCWWGG 1 cut(s) 849
EcoT22I ATGCAT 1 cut(s) 689
ErhI CCWWGG 1 cut(s) 849
FaeI CATG 1 cut(s) 984
FalI AAGNNNNNCTT 2 cut(s) 977, 1009
FaqI GGGAC 2 cut(s) 223, 460
FatI CATG 1 cut(s) 980
FauNDI CATATG 2 cut(s) 112, 894
FbaI TGATCA 1 cut(s) 889
Fnu4HI GCNGC 2 cut(s) 341, 969
FokI GGATG 3 cut(s) 466, 551, 968
Fsp4HI GCNGC 2 cut(s) 341, 969
FspBI CTAG 3 cut(s) 177, 642, 729
GlaI GCGC 1 cut(s) 791
GluI GCNGC 2 cut(s) 341, 969
GsaI CCCAGC 1 cut(s) 17
HaeII RGCGCY 1 cut(s) 793
HaeIII GGCC 1 cut(s) 234
HgaI GACGC 1 cut(s) 220
HhaI GCGC 1 cut(s) 792
Hin1II CATG 1 cut(s) 984
Hin6I GCGC 1 cut(s) 790
HinP1I GCGC 1 cut(s) 790
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 4 cut(s) 137, 492, 511, 931
HphI GGTGA 3 cut(s) 38, 92, 194
Hpy166II GTNNAC 3 cut(s) 94, 322, 622
Hpy188I TCNGA 4 cut(s) 66, 162, 295, 880
Hpy188III TCNNGA 5 cut(s) 38, 314, 466, 692, 914
Hpy8I GTNNAC 3 cut(s) 94, 322, 622
HpyAV CCTTC 3 cut(s) 438, 529, 803
HpyCH4III ACNGT 3 cut(s) 131, 202, 1007
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 6 cut(s) 50, 242, 572, 622, 687, 800
HpyF10VI GCNNNNNNNGC 3 cut(s) 524, 659, 787
HpyF3I CTNAG 2 cut(s) 402, 877
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 1 cut(s) 984
HspAI GCGC 1 cut(s) 790
Ksp22I TGATCA 1 cut(s) 889
Kzo9I GATC 4 cut(s) 61, 259, 716, 889
LmnI GCTCC 3 cut(s) 523, 778, 976
Lsp1109I GCAGC 2 cut(s) 352, 955
LweI GCATC 1 cut(s) 661
MabI ACCWGGT 1 cut(s) 223
MaeI CTAG 3 cut(s) 177, 642, 729
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 2 cut(s) 923, 1001
MalI GATC 4 cut(s) 63, 261, 718, 891
MboI GATC 4 cut(s) 61, 259, 716, 889
MboII GAAGA 4 cut(s) 358, 602, 929, 985
MfeI CAATTG 2 cut(s) 150, 997
MhlI GDGCHC 2 cut(s) 520, 624
MluCI AATT 5 cut(s) 150, 289, 351, 599, 997
MlyI GAGTC 2 cut(s) 505, 940
Mph1103I ATGCAT 1 cut(s) 689
MseI TTAA 2 cut(s) 276, 936
MspCI CTTAAG 1 cut(s) 935
MspR9I CCNGG 2 cut(s) 225, 307
MunI CAATTG 2 cut(s) 150, 997
Mva1269I GAATGC 2 cut(s) 17, 687
MvaI CCWGG 2 cut(s) 225, 307
MwoI GCNNNNNNNGC 3 cut(s) 524, 659, 787
NdeI CATATG 2 cut(s) 112, 894
NdeII GATC 4 cut(s) 61, 259, 716, 889
NlaIII CATG 1 cut(s) 984
NlaIV GGNNCC 1 cut(s) 808
NsiI ATGCAT 1 cut(s) 689
PctI GAATGC 2 cut(s) 17, 687
PfeI GAWTC 2 cut(s) 137, 492
PkrI GCNGC 2 cut(s) 342, 970
PleI GAGTC 2 cut(s) 505, 939
PpsI GAGTC 2 cut(s) 505, 939
PpuMI RGGWCCY 1 cut(s) 826
Psp5II RGGWCCY 1 cut(s) 826
Psp6I CCWGG 2 cut(s) 223, 305
PspFI CCCAGC 1 cut(s) 13
PspGI CCWGG 2 cut(s) 223, 305
PspN4I GGNNCC 1 cut(s) 808
PspPI GGNCC 2 cut(s) 425, 826
PspPPI RGGWCCY 1 cut(s) 826
PstI CTGCAG 1 cut(s) 244
PstNI CAGNNNCTG 1 cut(s) 674
SaqAI TTAA 2 cut(s) 276, 936
SatI GCNGC 2 cut(s) 341, 969
Sau3AI GATC 4 cut(s) 61, 259, 716, 889
Sau96I GGNCC 2 cut(s) 425, 826
SchI GAGTC 2 cut(s) 505, 940
ScrFI CCNGG 2 cut(s) 225, 307
SduI GDGCHC 2 cut(s) 520, 624
SexAI ACCWGGT 1 cut(s) 223
SfaNI GCATC 1 cut(s) 661
SfcI CTRYAG 1 cut(s) 240
SinI GGWCC 2 cut(s) 425, 826
SmlI CTYRAG 4 cut(s) 314, 605, 782, 935
SmoI CTYRAG 4 cut(s) 314, 605, 782, 935
SpeI ACTAGT 2 cut(s) 176, 641
Sse9I AATT 5 cut(s) 150, 289, 351, 599, 997
SsiI CCGC 2 cut(s) 78, 662
SspI AATATT 1 cut(s) 267
SspMI CTAG 3 cut(s) 177, 642, 729
StyD4I CCNGG 2 cut(s) 223, 305
StyI CCWWGG 1 cut(s) 849
TaaI ACNGT 3 cut(s) 131, 202, 1007
TaiI ACGT 1 cut(s) 99
TaqI TCGA 2 cut(s) 262, 561
TasI AATT 5 cut(s) 150, 289, 351, 599, 997
TfiI GAWTC 2 cut(s) 137, 492
Tru1I TTAA 2 cut(s) 276, 936
Tru9I TTAA 2 cut(s) 276, 936
TscAI CASTG 3 cut(s) 249, 403, 726
TseI GCWGC 2 cut(s) 340, 968
TspDTI ATGAA 3 cut(s) 536, 547, 972
TspGWI ACGGA 2 cut(s) 79, 639
TspRI CASTG 3 cut(s) 249, 403, 726
Vha464I CTTAAG 1 cut(s) 935
VneI GTGCAC 1 cut(s) 620
VpaK11BI GGWCC 2 cut(s) 425, 826
XapI RAATTY 3 cut(s) 289, 351, 599
XspI CTAG 3 cut(s) 177, 642, 729
Zsp2I ATGCAT 1 cut(s) 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.