RchiOBHm_Chr3g0481461

Guanylate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0004677
Physical Location & Seq
Reverse (-)
27501473 .. 27505259
3787 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44641

Sequence Viewer

Length: 1200 bp
ATGGGAGAAGCCCCAGCATTCTTTGGTGATGAGCTTCAGAAGGGATATGCAAATGGGTTTGATCTGAAGTCCAAAGGCGGTGAAACATCCATTGTCGTGGGCAACAGAACATATGTGATTGGTGGAGACTGTGAGGAATCAGCATTATCAATTGGAGTTCGGATTTTTGACAAAACTAGTGGTGAATGGATAAATCCTACTGTGTTGGGGACTCAACCCAAACCAGGTAAAGGCCATTCTGCAGTGCTTATGAGTGGAGATCGAATATTGATTATTAAGAGTGGTTCAAATTCAGATGATTTCGCCTGGTTTCTTGAGGTGGACACCCAATATGTTAGGCAGCAGAAGAAAATTTTAGGCACAGAGGTTGTTGCTTGGAGTAGGGGTGTGAGAGGCAGTGCTGAGAAGCCAGTAGTTATTAGTGGTCCTTCTGGGGTAGGTAAGGGGACACTAATATCAATGCTCATGAAAGAATATCCATCCCTGTTTGGATTCTCTGTGAGCCACACAACTCGTGCTCCAAGAGCTATGGAGAAGGATGGGGTTCATTATCATTTCATCGAGCGAAGTGCAATGGAGAAAGAGATAGAAGATGGGAAATTCCTTGAGTTTGCTTCTGTGCACGGAAATCTCTATGGAACTAGTGTTGAAGCAGTAGAAGCGGTAGCAGATGCTGGAAAGATATGCATTCTTGACATTGATGTTCAAGGGGCAAAATCAGTGAGGGCTACTTCGCTTGAAGCTCTCTTTATCTTTGTGTGTCCTCCCTCGATGGAGGAGCTTGAGAAGCGCCTTCGTGCAAGGGGAACCGAGACAGAGGAACAGGTCCTAAAGAGACTTCGAAATGCCAAGTCAGAGATTGAGCAGGGGCAATCATCAGGCATCTTTGGTCATATGTTGTATAATGATAATCTTGAAGATTGTTACAAGAGTCTTAAGGAACTTTTGGGGCTGGATGAAAGTATGGCTGCTCCTAAATCATCATCTAAAGTGGTTGATCTGTTTATCGACCATTCCGTTTCCAAGATTGACAACAAAATCATCATAAACTCCACAAACCCAGAACTGGAAAAGTCATCGACCAACATGATTGTGCTTGATGTGTCATCCCTCAAGGGAGGATCGCCTGGACGGACAAGAGGACTAAACATCTATACAATGGACTCAATTTTGGATGGTTGGGCTGGGATCCCACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004385 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006163 GO:0006164 GO:0006185 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009126 GO:0009132 GO:0009133 GO:0009135 GO:0009136 GO:0009141 GO:0009144 GO:0009150 GO:0009151 GO:0009152 GO:0009153 GO:0009161 GO:0009162 GO:0009163 GO:0009165 GO:0009167 GO:0009170 GO:0009179 GO:0009180 GO:0009182 GO:0009183 GO:0009185 GO:0009186 GO:0009188 GO:0009189 GO:0009199 GO:0009200 GO:0009205 GO:0009215 GO:0009225 GO:0009259 GO:0009260 GO:0009262 GO:0009263 GO:0009265 GO:0009394 GO:0009987 GO:0015031 GO:0015833 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016776 GO:0017144 GO:0018130 GO:0019205 GO:0019438 GO:0019637 GO:0019673 GO:0019692 GO:0019693 GO:0032501 GO:0032502 GO:0033036 GO:0034404 GO:0034436 GO:0034641 GO:0034654 GO:0040008 GO:0042278 GO:0042451 GO:0042455 GO:0042886 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046034 GO:0046037 GO:0046054 GO:0046060 GO:0046066 GO:0046128 GO:0046129 GO:0046385 GO:0046390 GO:0046483 GO:0046710 GO:0046711 GO:0046939 GO:0046940 GO:0048229 GO:0048638 GO:0048856 GO:0050145 GO:0050789 GO:0050793 GO:0051179 GO:0051234 GO:0055086 GO:0065007 GO:0071702 GO:0071704 GO:0071705 GO:0072521 GO:0072522 GO:0090407 GO:1901068 GO:1901070 GO:1901135 GO:1901137 GO:1901264 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659
KEGG Pathways
Metabolic & Signaling
ko00230 ko01100 map00230 map01100
Pfam Domains
Protein Families
Guanylate_kin

Protein Analysis

399

Amino Acids

43.39

Weight (kDa)

5.46

Isoelectric Point (pI)

33.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_FBX42 PF13415 26 - 108 1.9e-09 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 28 - 97 4.6e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Guanylate_kin PF00625 135 - 316 5.4e-60 Guanylate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 78, 662
AclWI GGATC 3 cut(s) 1129, 1183, 1196
AcsI RAATTY 3 cut(s) 289, 351, 599
AcuI CTGAAG 2 cut(s) 20, 86
AfiI CCNNNNNNNGG 3 cut(s) 224, 230, 1066
AflII CTTAAG 1 cut(s) 935
AgsI TTSAA 5 cut(s) 288, 650, 707, 740, 917
AhlI ACTAGT 2 cut(s) 176, 641
AjnI CCWGG 3 cut(s) 223, 305, 1126
AluBI AGCT 4 cut(s) 34, 527, 743, 781
AluI AGCT 4 cut(s) 34, 527, 743, 781
Alw21I GWGCWC 2 cut(s) 520, 624
Alw26I GTCTC 3 cut(s) 120, 806, 829
Alw44I GTGCAC 1 cut(s) 620
AlwI GGATC 3 cut(s) 1129, 1183, 1196
AlwNI CAGNNNCTG 1 cut(s) 674
AoxI GGCC 1 cut(s) 232
ApaLI GTGCAC 1 cut(s) 620
ApeKI GCWGC 2 cut(s) 340, 968
ApoI RAATTY 3 cut(s) 289, 351, 599
AspLEI GCGC 1 cut(s) 792
AspS9I GGNCC 2 cut(s) 425, 826
AsuHPI GGTGA 3 cut(s) 38, 92, 194
AsuII TTCGAA 1 cut(s) 841
AvaII GGWCC 2 cut(s) 425, 826
BaeGI GKGCMC 1 cut(s) 624
BamHI GGATCC 1 cut(s) 1188
BauI CACGAG 1 cut(s) 513
Bbv12I GWGCWC 2 cut(s) 520, 624
BbvI GCAGC 2 cut(s) 352, 955
BccI CCATC 5 cut(s) 487, 533, 587, 766, 1169
BcgI CGANNNNNNTGC 2 cut(s) 551, 585
BciT130I CCWGG 3 cut(s) 225, 307, 1128
BcoDI GTCTC 3 cut(s) 120, 806, 829
BcuI ACTAGT 2 cut(s) 176, 641
BfaI CTAG 2 cut(s) 177, 642
BfmI CTRYAG 1 cut(s) 240
BfoI RGCGCY 1 cut(s) 793
BfrI CTTAAG 1 cut(s) 935
BisI GCNGC 2 cut(s) 341, 969
BlsI GCNGC 2 cut(s) 342, 970
Bme1390I CCNGG 3 cut(s) 225, 307, 1128
Bme18I GGWCC 2 cut(s) 425, 826
BmgT120I GGNCC 2 cut(s) 425, 826
BmiI GGNNCC 2 cut(s) 808, 1190
BmrFI CCNGG 3 cut(s) 225, 307, 1128
BmsI GCATC 2 cut(s) 661, 891
Bpu14I TTCGAA 1 cut(s) 841
BpuEI CTTGAG 4 cut(s) 335, 626, 803, 1097
BsaXI ACNNNNNCTCC 4 cut(s) 370, 400, 502, 532
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 230, 1066
Bse1I ACTGG 2 cut(s) 410, 1071
Bse3DI GCAATG 1 cut(s) 579
BseBI CCWGG 3 cut(s) 225, 307, 1128
BseGI GGATG 6 cut(s) 86, 479, 544, 961, 1106, 1180
BseLI CCNNNNNNNGG 3 cut(s) 224, 230, 1066
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 1 cut(s) 393
BseNI ACTGG 2 cut(s) 410, 1071
BseRI GAGGAG 1 cut(s) 791
BseSI GKGCMC 1 cut(s) 624
BseXI GCAGC 2 cut(s) 352, 955
BseYI CCCAGC 2 cut(s) 13, 1184
BshFI GGCC 1 cut(s) 234
BsiHKAI GWGCWC 2 cut(s) 520, 624
BslFI GGGAC 2 cut(s) 223, 460
BslI CCNNNNNNNGG 3 cut(s) 224, 230, 1066
BsmAI GTCTC 3 cut(s) 120, 806, 829
BsmFI GGGAC 2 cut(s) 223, 460
BsmI GAATGC 2 cut(s) 17, 687
BsnI GGCC 1 cut(s) 234
Bsp119I TTCGAA 1 cut(s) 841
Bsp1286I GDGCHC 2 cut(s) 520, 624
Bsp143I GATC 5 cut(s) 61, 259, 997, 1121, 1188
BspACI CCGC 2 cut(s) 78, 662
BspANI GGCC 1 cut(s) 234
BspCNI CTCAG 1 cut(s) 394
BspHI TCATGA 1 cut(s) 465
BspLI GGNNCC 2 cut(s) 808, 1190
BspMAI CTGCAG 1 cut(s) 244
BspPI GGATC 3 cut(s) 1129, 1183, 1196
BspT104I TTCGAA 1 cut(s) 841
BspTI CTTAAG 1 cut(s) 935
BsrDI GCAATG 1 cut(s) 579
BsrI ACTGG 2 cut(s) 410, 1071
BssMI GATC 5 cut(s) 61, 259, 997, 1121, 1188
BssSI CACGAG 1 cut(s) 513
Bst2BI CACGAG 1 cut(s) 513
Bst2UI CCWGG 3 cut(s) 225, 307, 1128
Bst4CI ACNGT 2 cut(s) 131, 202
BstAFI CTTAAG 1 cut(s) 935
BstBI TTCGAA 1 cut(s) 841
BstDEI CTNAG 1 cut(s) 402
BstF5I GGATG 6 cut(s) 86, 479, 544, 961, 1106, 1180
BstH2I RGCGCY 1 cut(s) 793
BstHHI GCGC 1 cut(s) 792
BstKTI GATC 5 cut(s) 64, 262, 1000, 1124, 1191
BstMAI GTCTC 3 cut(s) 120, 806, 829
BstMBI GATC 5 cut(s) 61, 259, 997, 1121, 1188
BstMWI GCNNNNNNNGC 3 cut(s) 524, 659, 787
BstNI CCWGG 3 cut(s) 225, 307, 1128
BstSCI CCNGG 3 cut(s) 223, 305, 1126
BstSFI CTRYAG 1 cut(s) 240
BstSLI GKGCMC 1 cut(s) 624
BstV1I GCAGC 2 cut(s) 352, 955
BstX2I RGATCY 1 cut(s) 1188
BstXI CCANNNNNNTGG 1 cut(s) 97
BstYI RGATCY 1 cut(s) 1188
BsuRI GGCC 1 cut(s) 234
BtsCI GGATG 6 cut(s) 86, 479, 544, 961, 1106, 1180
BtsI GCAGTG 2 cut(s) 249, 403
BtsIMutI CAGTG 3 cut(s) 249, 403, 726
CaiI CAGNNNCTG 1 cut(s) 674
CciI TCATGA 1 cut(s) 465
CfoI GCGC 1 cut(s) 792
Cfr13I GGNCC 2 cut(s) 425, 826
CsiI ACCWGGT 1 cut(s) 223
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviAII CATG 2 cut(s) 466, 1087
DdeI CTNAG 1 cut(s) 402
DpnI GATC 5 cut(s) 63, 261, 999, 1123, 1190
DpnII GATC 5 cut(s) 61, 259, 997, 1121, 1188
Eco47I GGWCC 2 cut(s) 425, 826
Eco57I CTGAAG 2 cut(s) 20, 86
EcoO109I RGGNCCY 1 cut(s) 826
EcoRII CCWGG 3 cut(s) 223, 305, 1126
EcoT22I ATGCAT 1 cut(s) 689
FaeI CATG 2 cut(s) 469, 1090
FaqI GGGAC 2 cut(s) 223, 460
FatI CATG 2 cut(s) 465, 1086
FauNDI CATATG 2 cut(s) 112, 894
Fnu4HI GCNGC 2 cut(s) 341, 969
FokI GGATG 6 cut(s) 73, 466, 551, 968, 1093, 1187
Fsp4HI GCNGC 2 cut(s) 341, 969
FspBI CTAG 2 cut(s) 177, 642
GlaI GCGC 1 cut(s) 791
GluI GCNGC 2 cut(s) 341, 969
GsaI CCCAGC 2 cut(s) 17, 1188
HaeII RGCGCY 1 cut(s) 793
HaeIII GGCC 1 cut(s) 234
HhaI GCGC 1 cut(s) 792
Hin1II CATG 2 cut(s) 469, 1090
Hin6I GCGC 1 cut(s) 790
HinP1I GCGC 1 cut(s) 790
HinfI GANTC 5 cut(s) 137, 211, 492, 931, 1163
HphI GGTGA 3 cut(s) 38, 92, 194
Hpy166II GTNNAC 2 cut(s) 322, 622
Hpy188I TCNGA 5 cut(s) 39, 66, 162, 295, 856
Hpy188III TCNNGA 4 cut(s) 314, 466, 692, 914
Hpy8I GTNNAC 2 cut(s) 322, 622
HpyAV CCTTC 4 cut(s) 34, 438, 529, 803
HpyCH4III ACNGT 2 cut(s) 131, 202
HpyCH4V TGCA 6 cut(s) 50, 242, 572, 622, 687, 800
HpyF10VI GCNNNNNNNGC 3 cut(s) 524, 659, 787
HpyF3I CTNAG 1 cut(s) 402
Hsp92II CATG 2 cut(s) 469, 1090
HspAI GCGC 1 cut(s) 790
Kzo9I GATC 5 cut(s) 61, 259, 997, 1121, 1188
LmnI GCTCC 3 cut(s) 523, 778, 976
Lsp1109I GCAGC 2 cut(s) 352, 955
LweI GCATC 2 cut(s) 661, 891
MabI ACCWGGT 1 cut(s) 223
MaeI CTAG 2 cut(s) 177, 642
MaeIII GTNAC 1 cut(s) 923
MalI GATC 5 cut(s) 63, 261, 999, 1123, 1190
MboI GATC 5 cut(s) 61, 259, 997, 1121, 1188
MboII GAAGA 3 cut(s) 358, 602, 929
MfeI CAATTG 1 cut(s) 150
MflI RGATCY 1 cut(s) 1188
MhlI GDGCHC 2 cut(s) 520, 624
MluCI AATT 5 cut(s) 150, 289, 351, 599, 1167
MlyI GAGTC 3 cut(s) 205, 940, 1157
Mph1103I ATGCAT 1 cut(s) 689
MseI TTAA 2 cut(s) 276, 936
MslI CAYNNNNRTG 2 cut(s) 95, 1091
MspCI CTTAAG 1 cut(s) 935
MspR9I CCNGG 3 cut(s) 225, 307, 1128
MunI CAATTG 1 cut(s) 150
Mva1269I GAATGC 2 cut(s) 17, 687
MvaI CCWGG 3 cut(s) 225, 307, 1128
MwoI GCNNNNNNNGC 3 cut(s) 524, 659, 787
NdeI CATATG 2 cut(s) 112, 894
NdeII GATC 5 cut(s) 61, 259, 997, 1121, 1188
NlaIII CATG 2 cut(s) 469, 1090
NlaIV GGNNCC 2 cut(s) 808, 1190
NsiI ATGCAT 1 cut(s) 689
NspV TTCGAA 1 cut(s) 841
PagI TCATGA 1 cut(s) 465
PcsI WCGNNNNNNNCGW 1 cut(s) 1014
PctI GAATGC 2 cut(s) 17, 687
PfeI GAWTC 2 cut(s) 137, 492
PkrI GCNGC 2 cut(s) 342, 970
PleI GAGTC 3 cut(s) 205, 939, 1157
PpsI GAGTC 3 cut(s) 205, 939, 1157
PpuMI RGGWCCY 1 cut(s) 826
Psp5II RGGWCCY 1 cut(s) 826
Psp6I CCWGG 3 cut(s) 223, 305, 1126
PspFI CCCAGC 2 cut(s) 13, 1184
PspGI CCWGG 3 cut(s) 223, 305, 1126
PspN4I GGNNCC 2 cut(s) 808, 1190
PspPI GGNCC 2 cut(s) 425, 826
PspPPI RGGWCCY 1 cut(s) 826
PstI CTGCAG 1 cut(s) 244
PstNI CAGNNNCTG 1 cut(s) 674
PsuI RGATCY 1 cut(s) 1188
RseI CAYNNNNRTG 2 cut(s) 95, 1091
SaqAI TTAA 2 cut(s) 276, 936
SatI GCNGC 2 cut(s) 341, 969
Sau3AI GATC 5 cut(s) 61, 259, 997, 1121, 1188
Sau96I GGNCC 2 cut(s) 425, 826
SchI GAGTC 3 cut(s) 205, 940, 1157
ScrFI CCNGG 3 cut(s) 225, 307, 1128
SduI GDGCHC 2 cut(s) 520, 624
SetI ASST 9 cut(s) 36, 229, 321, 369, 442, 529, 745, 783, 828
SexAI ACCWGGT 1 cut(s) 223
SfaNI GCATC 2 cut(s) 661, 891
SfcI CTRYAG 1 cut(s) 240
SfuI TTCGAA 1 cut(s) 841
SinI GGWCC 2 cut(s) 425, 826
SmiMI CAYNNNNRTG 2 cut(s) 95, 1091
SmlI CTYRAG 5 cut(s) 314, 605, 782, 935, 1112
SmoI CTYRAG 5 cut(s) 314, 605, 782, 935, 1112
SpeI ACTAGT 2 cut(s) 176, 641
Sse9I AATT 5 cut(s) 150, 289, 351, 599, 1167
SsiI CCGC 2 cut(s) 78, 662
SspI AATATT 1 cut(s) 267
SspMI CTAG 2 cut(s) 177, 642
StyD4I CCNGG 3 cut(s) 223, 305, 1126
TaaI ACNGT 2 cut(s) 131, 202
TaqI TCGA 6 cut(s) 262, 561, 770, 841, 1008, 1079
TasI AATT 5 cut(s) 150, 289, 351, 599, 1167
TfiI GAWTC 2 cut(s) 137, 492
Tru1I TTAA 2 cut(s) 276, 936
Tru9I TTAA 2 cut(s) 276, 936
TscAI CASTG 3 cut(s) 249, 403, 726
TseI GCWGC 2 cut(s) 340, 968
TspDTI ATGAA 4 cut(s) 482, 536, 547, 972
TspGWI ACGGA 3 cut(s) 639, 1006, 1147
TspRI CASTG 3 cut(s) 249, 403, 726
Vha464I CTTAAG 1 cut(s) 935
VneI GTGCAC 1 cut(s) 620
VpaK11BI GGWCC 2 cut(s) 425, 826
XapI RAATTY 3 cut(s) 289, 351, 599
XspI CTAG 2 cut(s) 177, 642
Zsp2I ATGCAT 1 cut(s) 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.