pycom12g06050

Guanylate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0004677
Physical Location & Seq
Reverse (-)
6115671 .. 6118084
2414 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g06050.6

Sequence Viewer

Length: 1203 bp
ATGGGAGAAGCACCAGCATTCTTTTTTGATGTGCTGCCGAAGGGATTTGCAAATGGTTTTGATGTAAAATCAAAGGGCGGTGAAACGTCCGTTGTTGTGGGCAACAGAACATATGTGATTGGTGGAGCCTGTGAGGAATCGGCATTGTCAATTGGAGTTTGTATTCTTGACCAATCCACTGGTGAATGGATAAATCCTGTCGTGTTGGGAACCAAACCCAGGCCATCTAAAGGCCATTCAGCAGTGCTTTTAAGTGAAGACCGGATATTGATTACCAAGAATGGTTCCAGCACAGACGTTTTTGCCTGGTTTCTTGAGGTGGACACCCAATACGTTAGGCAGCAGAAGAAGATTTTGGGCACTGAGGTGGTTGCGTGGAGTAAGGGAGTGAGGGGCTATGCTGAGAAGCCAATTGTTATTAGTGGTCCTTCCGGGGTAGGAAAGGGGACACTAATATCCATGCTCATGAAGGAGTATCCATCCATGTTTGGATTCTCGGTGAGCCACACAACCCGTGCTCCAAGAGCTATGGAGAAGGATGGGGTCCATTACCATTTCATTGAGAGGAGTGTCATGGACAAAGAAATAAAAGACGGCAAGTTCCTTGAGTTCGCTTCTGTGCATGGAAATCTCTACGGAACTAGTGTTGAAGCAGTAGAAGTCGTAGCAGATGCTGGGAAGAGATGCATTCTTGACATCGATGTTCAAGGGGCACGATCCGTAAGGGCTAGTTCACTTGAAGCTATCTTCATCTTCGTATGTCCACCCTCAATGGAAGAGCTTGAGAAGCGCCTTCGGGCAAGGGGAACTGAGACAGAGGAACAGGTCCTAAAGCGACTTCGTAACGCCAATGCAGAGATTAAGCAAGGGCAATCATCAGGCATTTTTGATCATATGTTGTATAATGATAACCTGGAAGACTGTTACCAGAGTCTAAAGAAACTCTTGGGGCTAGATGGAACTGTTACTGCTGCTCCTAAATCGGCAAGTAAAGTGGTTGATCTGCCAATGGACCATTCGTTGACCAAGATTGATAACAAAATCATCATAAAGTCCAGAACTCCAGAACTGGAAAAAGCATCCACTAACACGATCGTGCTGGATGTGTCAGCCCTCAAAGGAGGGGCGCCTGGGCGGACTAGAGGGCTAGACATATATACCATGGACTCATTTTCAGACGGTCTGACTGGGATTAATCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004385 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006163 GO:0006164 GO:0006185 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009126 GO:0009132 GO:0009133 GO:0009135 GO:0009136 GO:0009141 GO:0009144 GO:0009150 GO:0009151 GO:0009152 GO:0009153 GO:0009161 GO:0009162 GO:0009163 GO:0009165 GO:0009167 GO:0009170 GO:0009179 GO:0009180 GO:0009182 GO:0009183 GO:0009185 GO:0009186 GO:0009188 GO:0009189 GO:0009199 GO:0009200 GO:0009205 GO:0009215 GO:0009225 GO:0009259 GO:0009260 GO:0009262 GO:0009263 GO:0009265 GO:0009394 GO:0009987 GO:0015031 GO:0015833 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016776 GO:0017144 GO:0018130 GO:0019205 GO:0019438 GO:0019637 GO:0019673 GO:0019692 GO:0019693 GO:0032501 GO:0032502 GO:0033036 GO:0034404 GO:0034436 GO:0034641 GO:0034654 GO:0040008 GO:0042278 GO:0042451 GO:0042455 GO:0042886 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046034 GO:0046037 GO:0046054 GO:0046060 GO:0046066 GO:0046128 GO:0046129 GO:0046385 GO:0046390 GO:0046483 GO:0046710 GO:0046711 GO:0046939 GO:0046940 GO:0048229 GO:0048638 GO:0048856 GO:0050145 GO:0050789 GO:0050793 GO:0051179 GO:0051234 GO:0055086 GO:0065007 GO:0071702 GO:0071704 GO:0071705 GO:0072521 GO:0072522 GO:0090407 GO:1901068 GO:1901070 GO:1901135 GO:1901137 GO:1901264 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659
KEGG Pathways
Metabolic & Signaling
ko00230 ko01100 map00230 map01100
Pfam Domains
Protein Families
Guanylate_kin

Protein Analysis

401

Amino Acids

43.49

Weight (kDa)

6.42

Isoelectric Point (pI)

30.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1126
AciI CCGC 2 cut(s) 78, 1135
AclWI GGATC 1 cut(s) 711
AcyI GRCGYC 1 cut(s) 1127
AfiI CCNNNNNNNGG 2 cut(s) 219, 230
AgsI TTSAA 3 cut(s) 650, 707, 740
AhlI ACTAGT 1 cut(s) 641
AjnI CCWGG 4 cut(s) 218, 305, 912, 1129
AjuI GAANNNNNNNTTGG 4 cut(s) 338, 370, 1000, 1032
AleI CACNNNNGTG 2 cut(s) 365, 1094
AluBI AGCT 3 cut(s) 527, 743, 781
AluI AGCT 3 cut(s) 527, 743, 781
Alw21I GWGCWC 1 cut(s) 520
Alw26I GTCTC 1 cut(s) 806
AlwI GGATC 1 cut(s) 711
AlwNI CAGNNNCTG 1 cut(s) 674
AoxI GGCC 2 cut(s) 221, 232
ApeKI GCWGC 3 cut(s) 34, 340, 971
AseI ATTAAT 1 cut(s) 1194
AspLEI GCGC 2 cut(s) 792, 1129
AspS9I GGNCC 4 cut(s) 425, 544, 826, 1012
AsuC2I CCSGG 1 cut(s) 433
AsuHPI GGTGA 3 cut(s) 92, 194, 511
AvaII GGWCC 4 cut(s) 425, 544, 826, 1012
BaeGI GKGCMC 2 cut(s) 362, 715
BanI GGYRCC 1 cut(s) 1126
BbsI GAAGAC 2 cut(s) 264, 924
Bbv12I GWGCWC 1 cut(s) 520
BbvI GCAGC 3 cut(s) 21, 352, 958
BccI CCATC 4 cut(s) 232, 487, 533, 950
BceAI ACGGC 1 cut(s) 610
BciT130I CCWGG 4 cut(s) 220, 307, 914, 1131
BciVI GTATCC 1 cut(s) 486
BclI TGATCA 1 cut(s) 889
BcnI CCSGG 1 cut(s) 433
BcoDI GTCTC 1 cut(s) 806
BcuI ACTAGT 1 cut(s) 641
BfaI CTAG 5 cut(s) 642, 729, 953, 1140, 1148
BfoI RGCGCY 2 cut(s) 793, 1130
BfuI GTATCC 1 cut(s) 486
BisI GCNGC 3 cut(s) 35, 341, 972
BlsI GCNGC 3 cut(s) 36, 342, 973
Bme1390I CCNGG 5 cut(s) 220, 307, 433, 914, 1131
Bme18I GGWCC 4 cut(s) 425, 544, 826, 1012
BmgT120I GGNCC 4 cut(s) 425, 544, 826, 1012
BmiI GGNNCC 5 cut(s) 127, 211, 286, 545, 1128
BmrFI CCNGG 5 cut(s) 220, 307, 433, 914, 1131
BmrI ACTGGG 1 cut(s) 1197
BmsI GCATC 3 cut(s) 661, 674, 1088
BmuI ACTGGG 1 cut(s) 1197
BpiI GAAGAC 2 cut(s) 264, 924
BpmI CTGGAG 1 cut(s) 1047
BpuEI CTTGAG 3 cut(s) 335, 626, 803
BpuMI CCSGG 1 cut(s) 433
Bsa29I ATCGAT 1 cut(s) 699
BsaHI GRCGYC 1 cut(s) 1127
BsaJI CCNNGG 4 cut(s) 218, 432, 1130, 1161
BsaWI WCCGGW 1 cut(s) 261
BsaXI ACNNNNNCTCC 4 cut(s) 502, 532, 958, 988
Bsc4I CCNNNNNNNGG 2 cut(s) 219, 230
Bse1I ACTGG 3 cut(s) 184, 1074, 1192
BseBI CCWGG 4 cut(s) 220, 307, 914, 1131
BseCI ATCGAT 1 cut(s) 699
BseDI CCNNGG 4 cut(s) 218, 432, 1130, 1161
BseGI GGATG 4 cut(s) 479, 544, 1079, 1108
BseLI CCNNNNNNNGG 2 cut(s) 219, 230
BseMII CTCAG 3 cut(s) 354, 393, 801
BseNI ACTGG 3 cut(s) 184, 1074, 1192
BseRI GAGGAG 1 cut(s) 580
BseSI GKGCMC 2 cut(s) 362, 715
BseXI GCAGC 3 cut(s) 21, 352, 958
BseYI CCCAGC 1 cut(s) 674
Bsh1285I CGRYCG 1 cut(s) 1095
BshFI GGCC 2 cut(s) 223, 234
BshNI GGYRCC 1 cut(s) 1126
BshVI ATCGAT 1 cut(s) 699
BsiEI CGRYCG 1 cut(s) 1095
BsiHKAI GWGCWC 1 cut(s) 520
BsiSI CCGG 2 cut(s) 262, 432
BslFI GGGAC 1 cut(s) 460
BslI CCNNNNNNNGG 2 cut(s) 219, 230
BsmAI GTCTC 1 cut(s) 806
BsmFI GGGAC 1 cut(s) 460
BsmI GAATGC 2 cut(s) 17, 687
BsnI GGCC 2 cut(s) 223, 234
Bsp1286I GDGCHC 3 cut(s) 362, 520, 715
Bsp143I GATC 4 cut(s) 716, 889, 1000, 1092
Bsp19I CCATGG 1 cut(s) 1161
BspACI CCGC 2 cut(s) 78, 1135
BspANI GGCC 2 cut(s) 223, 234
BspCNI CTCAG 3 cut(s) 355, 394, 802
BspDI ATCGAT 1 cut(s) 699
BspHI TCATGA 1 cut(s) 465
BspLI GGNNCC 5 cut(s) 127, 211, 286, 545, 1128
BspPI GGATC 1 cut(s) 711
BspQI GCTCTTC 1 cut(s) 771
BspT107I GGYRCC 1 cut(s) 1126
BsrI ACTGG 3 cut(s) 184, 1074, 1192
BssECI CCNNGG 4 cut(s) 218, 432, 1130, 1161
BssMI GATC 4 cut(s) 716, 889, 1000, 1092
BssNI GRCGYC 1 cut(s) 1127
BssT1I CCWWGG 1 cut(s) 1161
Bst2UI CCWGG 4 cut(s) 220, 307, 914, 1131
Bst4CI ACNGT 3 cut(s) 923, 964, 1181
Bst6I CTCTTC 2 cut(s) 674, 771
BstACI GRCGYC 1 cut(s) 1127
BstDEI CTNAG 3 cut(s) 363, 402, 810
BstDSI CCRYGG 1 cut(s) 1161
BstF5I GGATG 4 cut(s) 479, 544, 1079, 1108
BstH2I RGCGCY 2 cut(s) 793, 1130
BstHHI GCGC 2 cut(s) 792, 1129
BstKTI GATC 4 cut(s) 719, 892, 1003, 1095
BstMAI GTCTC 1 cut(s) 806
BstMBI GATC 4 cut(s) 716, 889, 1000, 1092
BstMCI CGRYCG 1 cut(s) 1095
BstMWI GCNNNNNNNGC 2 cut(s) 524, 787
BstNI CCWGG 4 cut(s) 220, 307, 914, 1131
BstSCI CCNGG 5 cut(s) 218, 305, 431, 912, 1129
BstSLI GKGCMC 2 cut(s) 362, 715
BstV1I GCAGC 3 cut(s) 21, 352, 958
BstV2I GAAGAC 2 cut(s) 264, 924
BstXI CCANNNNNNTGG 1 cut(s) 179
Bsu15I ATCGAT 1 cut(s) 699
BsuI GTATCC 1 cut(s) 486
BsuRI GGCC 2 cut(s) 223, 234
BsuTUI ATCGAT 1 cut(s) 699
BtgI CCRYGG 1 cut(s) 1161
BtsCI GGATG 4 cut(s) 479, 544, 1079, 1108
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 3 cut(s) 177, 249, 360
CaiI CAGNNNCTG 1 cut(s) 674
CciI TCATGA 1 cut(s) 465
CfoI GCGC 2 cut(s) 792, 1129
Cfr13I GGNCC 4 cut(s) 425, 544, 826, 1012
ClaI ATCGAT 1 cut(s) 699
CspCI CAANNNNNGTGG 2 cut(s) 975, 1010
CviAII CATG 6 cut(s) 460, 466, 484, 574, 623, 1162
DdeI CTNAG 3 cut(s) 363, 402, 810
DinI GGCGCC 1 cut(s) 1128
DpnI GATC 4 cut(s) 718, 891, 1002, 1094
DpnII GATC 4 cut(s) 716, 889, 1000, 1092
Eam1104I CTCTTC 2 cut(s) 674, 771
EarI CTCTTC 2 cut(s) 674, 771
EciI GGCGGA 1 cut(s) 1150
Eco130I CCWWGG 1 cut(s) 1161
Eco47I GGWCC 4 cut(s) 425, 544, 826, 1012
EcoO109I RGGNCCY 1 cut(s) 826
EcoRII CCWGG 4 cut(s) 218, 305, 912, 1129
EcoT14I CCWWGG 1 cut(s) 1161
EcoT22I ATGCAT 1 cut(s) 689
EgeI GGCGCC 1 cut(s) 1128
EheI GGCGCC 1 cut(s) 1128
ErhI CCWWGG 1 cut(s) 1161
FaeI CATG 6 cut(s) 463, 469, 487, 577, 626, 1165
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FaqI GGGAC 1 cut(s) 460
FatI CATG 6 cut(s) 459, 465, 483, 573, 622, 1161
FauNDI CATATG 2 cut(s) 112, 894
FbaI TGATCA 1 cut(s) 889
Fnu4HI GCNGC 3 cut(s) 35, 341, 972
FokI GGATG 4 cut(s) 466, 551, 1066, 1115
Fsp4HI GCNGC 3 cut(s) 35, 341, 972
FspBI CTAG 5 cut(s) 642, 729, 953, 1140, 1148
GlaI GCGC 2 cut(s) 791, 1128
GluI GCNGC 3 cut(s) 35, 341, 972
GsaI CCCAGC 1 cut(s) 678
GsuI CTGGAG 1 cut(s) 1047
HaeII RGCGCY 2 cut(s) 793, 1130
HaeIII GGCC 2 cut(s) 223, 234
HapII CCGG 2 cut(s) 262, 432
HhaI GCGC 2 cut(s) 792, 1129
Hin1I GRCGYC 1 cut(s) 1127
Hin1II CATG 6 cut(s) 463, 469, 487, 577, 626, 1165
Hin6I GCGC 2 cut(s) 790, 1127
HinP1I GCGC 2 cut(s) 790, 1127
HincII GTYRAC 1 cut(s) 1023
HindII GTYRAC 1 cut(s) 1023
HinfI GANTC 4 cut(s) 137, 492, 931, 1166
HpaII CCGG 2 cut(s) 262, 432
HphI GGTGA 3 cut(s) 92, 194, 511
Hpy166II GTNNAC 4 cut(s) 322, 734, 764, 1023
Hpy188I TCNGA 2 cut(s) 1177, 1185
Hpy188III TCNNGA 6 cut(s) 167, 314, 466, 692, 1056, 1064
Hpy8I GTNNAC 4 cut(s) 322, 734, 764, 1023
HpyAV CCTTC 5 cut(s) 34, 438, 463, 529, 803
HpyCH4III ACNGT 3 cut(s) 923, 964, 1181
HpyCH4IV ACGT 3 cut(s) 86, 297, 333
HpyCH4V TGCA 4 cut(s) 50, 622, 687, 854
HpyF10VI GCNNNNNNNGC 2 cut(s) 524, 787
HpyF3I CTNAG 3 cut(s) 363, 402, 810
HpySE526I ACGT 3 cut(s) 86, 297, 333
Hsp92I GRCGYC 1 cut(s) 1127
Hsp92II CATG 6 cut(s) 463, 469, 487, 577, 626, 1165
HspAI GCGC 2 cut(s) 790, 1127
KasI GGCGCC 1 cut(s) 1126
Ksp22I TGATCA 1 cut(s) 889
Kzo9I GATC 4 cut(s) 716, 889, 1000, 1092
LguI GCTCTTC 1 cut(s) 771
LmnI GCTCC 3 cut(s) 125, 523, 979
Lsp1109I GCAGC 3 cut(s) 21, 352, 958
LweI GCATC 3 cut(s) 661, 674, 1088
MaeI CTAG 5 cut(s) 642, 729, 953, 1140, 1148
MaeII ACGT 3 cut(s) 86, 297, 333
MaeIII GTNAC 3 cut(s) 842, 923, 964
MalI GATC 4 cut(s) 718, 891, 1002, 1094
MboI GATC 4 cut(s) 716, 889, 1000, 1092
MboII GAAGA 8 cut(s) 269, 358, 361, 691, 739, 745, 788, 929
MfeI CAATTG 2 cut(s) 150, 411
MhlI GDGCHC 3 cut(s) 362, 520, 715
MluCI AATT 2 cut(s) 150, 411
Mly113I GGCGCC 1 cut(s) 1127
MlyI GAGTC 2 cut(s) 940, 1160
Mph1103I ATGCAT 1 cut(s) 689
MseI TTAA 3 cut(s) 251, 861, 1194
MslI CAYNNNNRTG 3 cut(s) 365, 464, 1094
MspI CCGG 2 cut(s) 262, 432
MspR9I CCNGG 5 cut(s) 220, 307, 433, 914, 1131
MunI CAATTG 2 cut(s) 150, 411
Mva1269I GAATGC 2 cut(s) 17, 687
MvaI CCWGG 4 cut(s) 220, 307, 914, 1131
MwoI GCNNNNNNNGC 2 cut(s) 524, 787
NarI GGCGCC 1 cut(s) 1127
NciI CCSGG 1 cut(s) 433
NcoI CCATGG 1 cut(s) 1161
NdeI CATATG 2 cut(s) 112, 894
NdeII GATC 4 cut(s) 716, 889, 1000, 1092
NlaIII CATG 6 cut(s) 463, 469, 487, 577, 626, 1165
NlaIV GGNNCC 5 cut(s) 127, 211, 286, 545, 1128
NsiI ATGCAT 1 cut(s) 689
OliI CACNNNNGTG 2 cut(s) 365, 1094
PagI TCATGA 1 cut(s) 465
PciSI GCTCTTC 1 cut(s) 771
PctI GAATGC 2 cut(s) 17, 687
PfeI GAWTC 2 cut(s) 137, 492
PkrI GCNGC 3 cut(s) 36, 342, 973
Ple19I CGATCG 1 cut(s) 1095
PleI GAGTC 2 cut(s) 939, 1160
PluTI GGCGCC 1 cut(s) 1130
PpsI GAGTC 2 cut(s) 939, 1160
PpuMI RGGWCCY 1 cut(s) 826
PshBI ATTAAT 1 cut(s) 1194
Psp5II RGGWCCY 1 cut(s) 826
Psp6I CCWGG 4 cut(s) 218, 305, 912, 1129
PspFI CCCAGC 1 cut(s) 674
PspGI CCWGG 4 cut(s) 218, 305, 912, 1129
PspN4I GGNNCC 5 cut(s) 127, 211, 286, 545, 1128
PspPI GGNCC 4 cut(s) 425, 544, 826, 1012
PspPPI RGGWCCY 1 cut(s) 826
PstNI CAGNNNCTG 1 cut(s) 674
PvuI CGATCG 1 cut(s) 1095
RseI CAYNNNNRTG 3 cut(s) 365, 464, 1094
SapI GCTCTTC 1 cut(s) 771
SaqAI TTAA 3 cut(s) 251, 861, 1194
SatI GCNGC 3 cut(s) 35, 341, 972
Sau3AI GATC 4 cut(s) 716, 889, 1000, 1092
Sau96I GGNCC 4 cut(s) 425, 544, 826, 1012
SchI GAGTC 2 cut(s) 940, 1160
ScrFI CCNGG 5 cut(s) 220, 307, 433, 914, 1131
SduI GDGCHC 3 cut(s) 362, 520, 715
SfaNI GCATC 3 cut(s) 661, 674, 1088
SfoI GGCGCC 1 cut(s) 1128
SinI GGWCC 4 cut(s) 425, 544, 826, 1012
SmiMI CAYNNNNRTG 3 cut(s) 365, 464, 1094
SmlI CTYRAG 3 cut(s) 314, 605, 782
SmoI CTYRAG 3 cut(s) 314, 605, 782
SpeI ACTAGT 1 cut(s) 641
Sse9I AATT 2 cut(s) 150, 411
SsiI CCGC 2 cut(s) 78, 1135
SspDI GGCGCC 1 cut(s) 1126
SspMI CTAG 5 cut(s) 642, 729, 953, 1140, 1148
StyD4I CCNGG 5 cut(s) 218, 305, 431, 912, 1129
StyI CCWWGG 1 cut(s) 1161
TaaI ACNGT 3 cut(s) 923, 964, 1181
TaiI ACGT 3 cut(s) 89, 300, 336
TaqI TCGA 1 cut(s) 699
TasI AATT 2 cut(s) 150, 411
TfiI GAWTC 2 cut(s) 137, 492
Tru1I TTAA 3 cut(s) 251, 861, 1194
Tru9I TTAA 3 cut(s) 251, 861, 1194
TscAI CASTG 3 cut(s) 184, 249, 367
TseI GCWGC 3 cut(s) 34, 340, 971
TspDTI ATGAA 3 cut(s) 482, 547, 739
TspGWI ACGGA 3 cut(s) 79, 651, 709
TspRI CASTG 3 cut(s) 184, 249, 367
VpaK11BI GGWCC 4 cut(s) 425, 544, 826, 1012
VspI ATTAAT 1 cut(s) 1194
XspI CTAG 5 cut(s) 642, 729, 953, 1140, 1148
Zsp2I ATGCAT 1 cut(s) 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.