AT3G59040

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007987
Physical Location & Seq
Reverse (-)
21821349 .. 21824425
3077 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G59040.1

Sequence Viewer

Length: 1752 bp
ATGTCACAGGCTGTAATTTTCAAGCCGTTTGTTTCAGTTCCATCAAGTAATCACAGCCAGAGGAAACTACAGAACAATATTATCAATGTTGGTGTTAAAATCCAAAACCGATTCAGAGTTGTTTGTATGGGAATGTTGGCTCCAAGGAAATTCCTGCAAAAGAGGAGGAAAATGGAGGTTTTCAAGGATGCAGCTGACGAGACGGATCAGAAGAGATGGAGAGGGCTTATGCTAGAGATTGAATCGACGGGTTCTGCTGTTCCGGTGCTTAGGCAATACAAAACTGACGGTGACCAAGGTCTTCCAAGGGATCTTGTTTTGGGTACTTTGGTTAGATTTAAGCAGCTTAAGAAATGGAACCTTGTCAGTGAGATTCTTGAATGGCTTAGATACCAGAACTGGTGGAATTTCAGTGAAATAGACTTTTTGATGCTCATTACAGCTTATGGGAAACTAGGAAACTTCAACGGAGCTGAAAGGGTTTTGAGTGTATTGAGTAAGATGGGCTCGACTCCAAACGTGATTTCTTATACCGCTCTCATGGAATCTTATGGGAGAGGAGGCAAATGCAACAATGCTGAAGCGATATTTCGAAGGATGCAGTCTTCAGGCCCAGAGCCTTCTGCTATAACTTATCAGATTATACTTAAGACTTTTGTAGAGGGGGACAAATTTAAAGAAGCTGAAGAGGTTTTTGAGACTCTTTTAGATGAGAAGAAATCTCCATTGAAGCCGGATCAAAAGATGTATCACATGATGATATACATGTACAAGAAGGCTGGCAACTATGAGAAAGCTCGAAAAGTATTTTCTTCAATGGTTGGGAAAGGAGTTCCCCAGTCTACAGTCACTTACAATAGCCTTATGTCTTTCGAAACTAGTTATAAAGAAGTTTCTAAGATCTATGACCAGATGCAAAGATCTGATATTCAACCCGACGTTGTAAGTTATGCTTTACTTATCAAAGCATATGGAAGAGCCAGAAGAGAGGAAGAAGCTCTATCTGTTTTTGAAGAGATGCTTGATGCTGGTGTAAGGCCAACACATAAAGCTTATAACATTTTGCTGGATGCATTTGCTATTTCTGGGATGGTGGAGCAAGCAAAGACTGTCTTTAAGAGCATGCGGCGGGACAGAATTTTCCCGGACCTCTGGTCGTACACAACTATGTTATCAGCATATGTGAATGCATCTGACATGGAGGGTGCTGAGAAATTCTTCAAGAGGATAAAAGTAGACGGTTTTGAACCGAATATAGTCACCTACGGGACACTGATAAAAGGGTATGCGAAAGCGAACGATGTTGAGAAGATGATGGAGGTATATGAGAAAATGAGGTTAAGTGGAATCAAAGCTAATCAAACCATCTTAACAACTATCATGGATGCGTCTGGGAGGTGCAAGAATTTCGGAAGTGCTCTTGGTTGGTATAAAGAGATGGAGAGCTGTGGGGTACCACCTGATCAGAAAGCTAAGAATGTGCTTTTATCATTGGCTTCAACTCAAGATGAGCTAGAGGAAGCTAAGGAACTTACTGGTATCAGAAATGAAACAGCAACCATTATTGCTAGAGTTTATGGATCTGATGATGATGAAGAGGGGGTAGAAGATATAAGTAGTGAATCTAGTGACGATGAGGATGAAGGAGATGACGATGATGATGATGCAAGAGAAACTGTGTTGTATGATAAACCACAAGAAGGGTCTTTGGGTTATGGTAGCTTACAGACGGAAGAACTTGTGGGGTTATGA

Protein Analysis

583

Amino Acids

66.16

Weight (kDa)

5.46

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 152 - 272 3.3e-08 Fungal tetratrico peptide repeats
PPR_2 PF13041 172 - 219 1.3e-09 PPR repeat family
PPR_3 PF13812 172 - 220 5.7e-08 Pentatricopeptide repeat domain
PPR_long PF17177 192 - 307 7.4e-06 Pentacotripeptide-repeat region of PRORP
PPR_long PF17177 241 - 365 2e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 245 - 289 2.5e-07 PPR repeat family
PPR_3 PF13812 300 - 359 7.5e-13 Pentatricopeptide repeat domain
PPR_2 PF13041 312 - 360 6.6e-12 PPR repeat family
PPR PF01535 315 - 345 2.3e-06 PPR repeat
PPR_2 PF13041 351 - 395 3e-09 PPR repeat family
TPR_24 PF23276 352 - 444 6.9e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 366 - 466 2.6e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 371 - 429 9.5e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 386 - 431 1.9e-11 PPR repeat family
PPR_3 PF13812 412 - 461 1.5e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 413 - 446 3.8e-08 PPR repeat
PPR_2 PF13041 417 - 454 1.3e-08 PPR repeat family
PPR PF01535 420 - 450 1.5e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 885, 1056
Acc65I GGTACC 1 cut(s) 1453
AccB1I GGYRCC 1 cut(s) 1453
AccBSI CCGCTC 1 cut(s) 536
AccI GTMKAC 2 cut(s) 842, 1236
AciI CCGC 3 cut(s) 534, 1126, 1129
AclWI GGATC 4 cut(s) 213, 318, 744, 1588
AcsI RAATTY 6 cut(s) 149, 406, 671, 1137, 1214, 1405
AcuI CTGAAG 3 cut(s) 591, 600, 705
AfaI GTAC 4 cut(s) 325, 770, 1160, 1455
AfiI CCNNNNNNNGG 1 cut(s) 1700
AflII CTTAAG 2 cut(s) 347, 647
AflIII ACRYGT 1 cut(s) 765
AhdI GACNNNNNGTC 1 cut(s) 1153
AhlI ACTAGT 1 cut(s) 878
AjuI GAANNNNNNNTTGG 2 cut(s) 508, 540
Alw21I GWGCWC 1 cut(s) 1420
Alw26I GTCTC 2 cut(s) 194, 692
AlwI GGATC 4 cut(s) 213, 318, 744, 1588
AoxI GGCC 2 cut(s) 610, 1037
ApeKI GCWGC 2 cut(s) 191, 343
ApoI RAATTY 6 cut(s) 149, 406, 671, 1137, 1214, 1405
Asp700I GAANNNNTTC 1 cut(s) 1217
Asp718I GGTACC 1 cut(s) 1453
AspS9I GGNCC 2 cut(s) 611, 1147
AsuC2I CCSGG 1 cut(s) 1145
AsuHPI GGTGA 2 cut(s) 302, 1252
AsuII TTCGAA 2 cut(s) 592, 873
AvaII GGWCC 1 cut(s) 1147
BaeI ACNNNNGTAYC 2 cut(s) 315, 348
BanI GGYRCC 1 cut(s) 1453
BanII GRGCYC 1 cut(s) 509
BbsI GAAGAC 2 cut(s) 293, 597
Bbv12I GWGCWC 1 cut(s) 1420
BbvI GCAGC 2 cut(s) 203, 355
BccI CCATC 7 cut(s) 49, 210, 496, 1084, 1309, 1373, 1432
BceAI ACGGC 1 cut(s) 10
BcgI CGANNNNNNTGC 2 cut(s) 1390, 1424
BclI TGATCA 1 cut(s) 1462
BcnI CCSGG 1 cut(s) 1145
BcoDI GTCTC 2 cut(s) 194, 692
BcuI ACTAGT 1 cut(s) 878
BfaI CTAG 6 cut(s) 233, 455, 879, 1514, 1569, 1626
BfmI CTRYAG 2 cut(s) 68, 843
BfrI CTTAAG 2 cut(s) 347, 647
BglII AGATCT 2 cut(s) 900, 920
BisI GCNGC 3 cut(s) 192, 344, 1127
BlsI GCNGC 3 cut(s) 193, 345, 1128
Bme1390I CCNGG 1 cut(s) 1145
Bme18I GGWCC 1 cut(s) 1147
BmeRI GACNNNNNGTC 1 cut(s) 1153
BmgT120I GGNCC 2 cut(s) 611, 1147
BmiI GGNNCC 3 cut(s) 141, 359, 1455
BmrFI CCNGG 1 cut(s) 1145
BmrI ACTGGG 1 cut(s) 832
BmuI ACTGGG 1 cut(s) 832
BoxI GACNNNNGTC 1 cut(s) 297
BpiI GAAGAC 2 cut(s) 293, 597
Bpu10I CCTNAGC 2 cut(s) 269, 1524
Bpu14I TTCGAA 2 cut(s) 592, 873
BpuEI CTTGAG 1 cut(s) 1488
BpuMI CCSGG 1 cut(s) 1145
BsaJI CCNNGG 3 cut(s) 143, 295, 305
BsaWI WCCGGW 1 cut(s) 262
Bsc4I CCNNNNNNNGG 1 cut(s) 1700
Bse1I ACTGG 3 cut(s) 404, 838, 1540
BseDI CCNNGG 3 cut(s) 143, 295, 305
BseGI GGATG 6 cut(s) 193, 603, 1075, 1095, 1390, 1645
BseLI CCNNNNNNNGG 1 cut(s) 1700
BseMII CTCAG 1 cut(s) 1200
BseNI ACTGG 3 cut(s) 404, 838, 1540
BseRI GAGGAG 2 cut(s) 178, 573
BseXI GCAGC 2 cut(s) 203, 355
BshFI GGCC 2 cut(s) 612, 1039
BshNI GGYRCC 1 cut(s) 1453
BsiHKAI GWGCWC 1 cut(s) 1420
BsiSI CCGG 3 cut(s) 263, 734, 1145
BslFI GGGAC 3 cut(s) 680, 1145, 1282
BslI CCNNNNNNNGG 1 cut(s) 1700
BsmAI GTCTC 2 cut(s) 194, 692
BsmBI CGTCTC 1 cut(s) 194
BsmFI GGGAC 3 cut(s) 680, 1145, 1282
BsmI GAATGC 1 cut(s) 1192
BsnI GGCC 2 cut(s) 612, 1039
Bsp119I TTCGAA 2 cut(s) 592, 873
Bsp1286I GDGCHC 2 cut(s) 509, 1420
Bsp1407I TGTACA 1 cut(s) 768
Bsp143I GATC 7 cut(s) 205, 310, 736, 900, 920, 1462, 1580
BspACI CCGC 3 cut(s) 534, 1126, 1129
BspANI GGCC 2 cut(s) 612, 1039
BspCNI CTCAG 1 cut(s) 1201
BspLI GGNNCC 3 cut(s) 141, 359, 1455
BspPI GGATC 4 cut(s) 213, 318, 744, 1588
BspQI GCTCTTC 1 cut(s) 970
BspT104I TTCGAA 2 cut(s) 592, 873
BspT107I GGYRCC 1 cut(s) 1453
BspTI CTTAAG 2 cut(s) 347, 647
BsrBI CCGCTC 1 cut(s) 536
BsrGI TGTACA 1 cut(s) 768
BsrI ACTGG 3 cut(s) 404, 838, 1540
BssECI CCNNGG 3 cut(s) 143, 295, 305
BssMI GATC 7 cut(s) 205, 310, 736, 900, 920, 1462, 1580
BssT1I CCWWGG 3 cut(s) 143, 295, 305
Bst4CI ACNGT 5 cut(s) 290, 847, 1111, 1241, 1678
Bst6I CTCTTC 6 cut(s) 206, 681, 970, 979, 1008, 1590
BstAFI CTTAAG 2 cut(s) 347, 647
BstAUI TGTACA 1 cut(s) 768
BstBI TTCGAA 2 cut(s) 592, 873
BstC8I GCNNGC 3 cut(s) 781, 1101, 1124
BstDEI CTNAG 6 cut(s) 269, 386, 897, 1209, 1473, 1524
BstEII GGTNACC 1 cut(s) 290
BstF5I GGATG 6 cut(s) 193, 603, 1075, 1095, 1390, 1645
BstKTI GATC 7 cut(s) 208, 313, 739, 903, 923, 1465, 1583
BstMAI GTCTC 2 cut(s) 194, 692
BstMBI GATC 7 cut(s) 205, 310, 736, 900, 920, 1462, 1580
BstNSI RCATGY 2 cut(s) 769, 1126
BstPAI GACNNNNGTC 1 cut(s) 297
BstPI GGTNACC 1 cut(s) 290
BstSCI CCNGG 1 cut(s) 1143
BstSFI CTRYAG 2 cut(s) 68, 843
BstV1I GCAGC 2 cut(s) 203, 355
BstV2I GAAGAC 2 cut(s) 293, 597
BstX2I RGATCY 4 cut(s) 310, 900, 920, 1580
BstYI RGATCY 4 cut(s) 310, 900, 920, 1580
BsuRI GGCC 2 cut(s) 612, 1039
BtsCI GGATG 6 cut(s) 193, 603, 1075, 1095, 1390, 1645
BtsIMutI CAGTG 3 cut(s) 373, 418, 1271
Cac8I GCNNGC 3 cut(s) 781, 1101, 1124
Cfr13I GGNCC 2 cut(s) 611, 1147
CseI GACGC 1 cut(s) 1377
Csp6I GTAC 4 cut(s) 324, 769, 1159, 1454
CviAII CATG 6 cut(s) 541, 754, 766, 1123, 1198, 1381
CviQI GTAC 4 cut(s) 324, 769, 1159, 1454
DdeI CTNAG 6 cut(s) 269, 386, 897, 1209, 1473, 1524
DpnI GATC 7 cut(s) 207, 312, 738, 902, 922, 1464, 1582
DpnII GATC 7 cut(s) 205, 310, 736, 900, 920, 1462, 1580
DraI TTTAAA 1 cut(s) 676
DriI GACNNNNNGTC 1 cut(s) 1153
Eam1104I CTCTTC 6 cut(s) 206, 681, 970, 979, 1008, 1590
Eam1105I GACNNNNNGTC 1 cut(s) 1153
EarI CTCTTC 6 cut(s) 206, 681, 970, 979, 1008, 1590
Eco130I CCWWGG 3 cut(s) 143, 295, 305
Eco24I GRGCYC 1 cut(s) 509
Eco47I GGWCC 1 cut(s) 1147
Eco57I CTGAAG 3 cut(s) 591, 600, 705
Eco91I GGTNACC 1 cut(s) 290
EcoO65I GGTNACC 1 cut(s) 290
EcoT14I CCWWGG 3 cut(s) 143, 295, 305
EcoT22I ATGCAT 2 cut(s) 1075, 1192
EcoT38I GRGCYC 1 cut(s) 509
ErhI CCWWGG 3 cut(s) 143, 295, 305
Esp3I CGTCTC 1 cut(s) 194
FaeI CATG 6 cut(s) 544, 757, 769, 1126, 1201, 1384
FalI AAGNNNNNCTT 6 cut(s) 937, 969, 1005, 1037, 1097, 1129
FaqI GGGAC 3 cut(s) 680, 1145, 1282
FatI CATG 6 cut(s) 540, 753, 765, 1122, 1197, 1380
FauI CCCGC 1 cut(s) 1122
FauNDI CATATG 2 cut(s) 970, 1180
FbaI TGATCA 1 cut(s) 1462
FblI GTMKAC 2 cut(s) 842, 1236
Fnu4HI GCNGC 3 cut(s) 192, 344, 1127
FokI GGATG 6 cut(s) 200, 610, 1082, 1102, 1397, 1652
FriOI GRGCYC 1 cut(s) 509
Fsp4HI GCNGC 3 cut(s) 192, 344, 1127
FspBI CTAG 6 cut(s) 233, 455, 879, 1514, 1569, 1626
GluI GCNGC 3 cut(s) 192, 344, 1127
HaeIII GGCC 2 cut(s) 612, 1039
HapII CCGG 3 cut(s) 263, 734, 1145
HgaI GACGC 1 cut(s) 1377
Hin1II CATG 6 cut(s) 544, 757, 769, 1126, 1201, 1384
HindIII AAGCTT 1 cut(s) 1050
HinfI GANTC 8 cut(s) 111, 242, 373, 511, 545, 700, 1347, 1622
HpaII CCGG 3 cut(s) 263, 734, 1145
HphI GGTGA 2 cut(s) 302, 1252
Hpy166II GTNNAC 3 cut(s) 843, 1161, 1237
Hpy188I TCNGA 9 cut(s) 116, 210, 639, 925, 1195, 1412, 1467, 1544, 1585
Hpy188III TCNNGA 3 cut(s) 377, 1222, 1505
Hpy8I GTNNAC 3 cut(s) 843, 1161, 1237
Hpy99I CGWCG 2 cut(s) 250, 941
HpyAV CCTTC 5 cut(s) 588, 630, 769, 1637, 1694
HpyCH4III ACNGT 5 cut(s) 290, 847, 1111, 1241, 1678
HpyCH4IV ACGT 2 cut(s) 519, 939
HpyCH4V TGCA 9 cut(s) 157, 191, 570, 601, 916, 1073, 1190, 1401, 1667
HpyF3I CTNAG 6 cut(s) 269, 386, 897, 1209, 1473, 1524
HpySE526I ACGT 2 cut(s) 519, 939
Hsp92II CATG 6 cut(s) 544, 757, 769, 1126, 1201, 1384
KpnI GGTACC 1 cut(s) 1457
Ksp22I TGATCA 1 cut(s) 1462
Kzo9I GATC 7 cut(s) 205, 310, 736, 900, 920, 1462, 1580
LguI GCTCTTC 1 cut(s) 970
LmnI GCTCC 3 cut(s) 145, 470, 1096
Lsp1109I GCAGC 2 cut(s) 203, 355
MaeI CTAG 6 cut(s) 233, 455, 879, 1514, 1569, 1626
MaeII ACGT 2 cut(s) 519, 939
MaeIII GTNAC 5 cut(s) 3, 290, 847, 1258, 1628
MalI GATC 7 cut(s) 207, 312, 738, 902, 922, 1464, 1582
MbiI CCGCTC 1 cut(s) 536
MboI GATC 7 cut(s) 205, 310, 736, 900, 920, 1462, 1580
MflI RGATCY 4 cut(s) 310, 900, 920, 1580
MhlI GDGCHC 2 cut(s) 509, 1420
MluCI AATT 7 cut(s) 15, 149, 406, 671, 1137, 1214, 1405
MlyI GAGTC 2 cut(s) 505, 694
Mph1103I ATGCAT 2 cut(s) 1075, 1192
MroXI GAANNNNTTC 1 cut(s) 1217
MseI TTAA 8 cut(s) 96, 339, 348, 648, 675, 1116, 1340, 1370
MslI CAYNNNNRTG 1 cut(s) 1166
MspA1I CMGCKG 1 cut(s) 194
MspCI CTTAAG 2 cut(s) 347, 647
MspI CCGG 3 cut(s) 263, 734, 1145
MspR9I CCNGG 1 cut(s) 1145
Mva1269I GAATGC 1 cut(s) 1192
NciI CCSGG 1 cut(s) 1145
NdeI CATATG 2 cut(s) 970, 1180
NdeII GATC 7 cut(s) 205, 310, 736, 900, 920, 1462, 1580
NlaIII CATG 6 cut(s) 544, 757, 769, 1126, 1201, 1384
NlaIV GGNNCC 3 cut(s) 141, 359, 1455
NmuCI GTSAC 5 cut(s) 3, 290, 847, 1258, 1628
NsiI ATGCAT 2 cut(s) 1075, 1192
NspI RCATGY 2 cut(s) 769, 1126
NspV TTCGAA 2 cut(s) 592, 873
PaeI GCATGC 1 cut(s) 1126
PciI ACATGT 1 cut(s) 765
PciSI GCTCTTC 1 cut(s) 970
PctI GAATGC 1 cut(s) 1192
PdmI GAANNNNTTC 1 cut(s) 1217
PfeI GAWTC 6 cut(s) 111, 242, 373, 545, 1347, 1622
PfoI TCCNGGA 1 cut(s) 1143
PkrI GCNGC 3 cut(s) 193, 345, 1128
PleI GAGTC 2 cut(s) 505, 694
PpsI GAGTC 2 cut(s) 505, 694
PscI ACATGT 1 cut(s) 765
PshAI GACNNNNGTC 1 cut(s) 297
PsiI TTATAA 2 cut(s) 885, 1056
PspEI GGTNACC 1 cut(s) 290
PspN4I GGNNCC 3 cut(s) 141, 359, 1455
PspPI GGNCC 2 cut(s) 611, 1147
PsuI RGATCY 4 cut(s) 310, 900, 920, 1580
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 4 cut(s) 325, 770, 1160, 1455
RsaNI GTAC 4 cut(s) 324, 769, 1159, 1454
RseI CAYNNNNRTG 1 cut(s) 1166
SapI GCTCTTC 1 cut(s) 970
SaqAI TTAA 8 cut(s) 96, 339, 348, 648, 675, 1116, 1340, 1370
SatI GCNGC 3 cut(s) 192, 344, 1127
Sau3AI GATC 7 cut(s) 205, 310, 736, 900, 920, 1462, 1580
Sau96I GGNCC 2 cut(s) 611, 1147
SchI GAGTC 2 cut(s) 505, 694
ScrFI CCNGG 1 cut(s) 1145
SduI GDGCHC 2 cut(s) 509, 1420
SfcI CTRYAG 2 cut(s) 68, 843
SfuI TTCGAA 2 cut(s) 592, 873
SinI GGWCC 1 cut(s) 1147
SmiMI CAYNNNNRTG 1 cut(s) 1166
SmlI CTYRAG 3 cut(s) 347, 647, 1503
SmoI CTYRAG 3 cut(s) 347, 647, 1503
SpeI ACTAGT 1 cut(s) 878
SphI GCATGC 1 cut(s) 1126
Sse9I AATT 7 cut(s) 15, 149, 406, 671, 1137, 1214, 1405
SsiI CCGC 3 cut(s) 534, 1126, 1129
SspI AATATT 1 cut(s) 79
SspMI CTAG 6 cut(s) 233, 455, 879, 1514, 1569, 1626
StyD4I CCNGG 1 cut(s) 1143
StyI CCWWGG 3 cut(s) 143, 295, 305
TaaI ACNGT 5 cut(s) 290, 847, 1111, 1241, 1678
TaiI ACGT 2 cut(s) 522, 942
TaqI TCGA 5 cut(s) 245, 509, 592, 799, 873
TasI AATT 7 cut(s) 15, 149, 406, 671, 1137, 1214, 1405
TatI WGTACW 1 cut(s) 768
TauI GCSGC 1 cut(s) 1129
TfiI GAWTC 6 cut(s) 111, 242, 373, 545, 1347, 1622
Tru1I TTAA 8 cut(s) 96, 339, 348, 648, 675, 1116, 1340, 1370
Tru9I TTAA 8 cut(s) 96, 339, 348, 648, 675, 1116, 1340, 1370
TscAI CASTG 3 cut(s) 373, 418, 1278
TseFI GTSAC 5 cut(s) 3, 290, 847, 1258, 1628
TseI GCWGC 2 cut(s) 191, 343
Tsp45I GTSAC 5 cut(s) 3, 290, 847, 1258, 1628
TspDTI ATGAA 3 cut(s) 1563, 1608, 1656
TspGWI ACGGA 3 cut(s) 218, 483, 1745
TspRI CASTG 3 cut(s) 373, 418, 1278
Vha464I CTTAAG 2 cut(s) 347, 647
VpaK11BI GGWCC 1 cut(s) 1147
XapI RAATTY 6 cut(s) 149, 406, 671, 1137, 1214, 1405
XceI RCATGY 2 cut(s) 769, 1126
XmiI GTMKAC 2 cut(s) 842, 1236
XmnI GAANNNNTTC 1 cut(s) 1217
XspI CTAG 6 cut(s) 233, 455, 879, 1514, 1569, 1626
Zsp2I ATGCAT 2 cut(s) 1075, 1192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.