Rh2DG518900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007987
Physical Location & Seq
Forward (+)
74240690 .. 74245140
4451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG518900.1

Sequence Viewer

Length: 1365 bp
ATGGATTTTCTGATGCTTATAACAGCTTATGGAAAGCAAGGCAAATTCAACAGGGCTGAGAAGGTTTTAAGCTTGTTGAATGAAAAGGGCTATGCACCAAGTGTAATATCGCATACTGCTCTTATGGAAGCATATGGAAAAGGAGGCCGATACAATAATGCTGAAGCAATATTTCGAAGAATGCAGTCTTCAGGGCCTGAACCATCTGCTGTGACATATCAAATTATACTAAAAATATTTGTTGAGGGCTGCAAGTTTAAGGAAGCTGAAGAAATCTTTGAGACCCTTTTGGATGAGGAAAAGTCACCTTTAAAGCCAGACCAAAAGATGTTCCACATGATGATCTATATGTTTAAGAAGGCTGGAAGTTATGAAAAAGCTCGTAAGATGTTTGCAGTGATGACTGAGAGAGGGGTTCGGCAATCAACAGTTACTTATAATAGCCTAATGTCGTTTGAAACTAACTACAAGGAAGTATCAAAGATGTACAACCAGATGCAAAGAGCTGGTCTCCGACCTGATGTTGTGAGTTATGCTTTACTCATTAATGCATATGGGAAAGCTAGAAGAGAGGAGGAAGCCTTAGCTGTTTTTGAGGAAATGCTTGATGCTGGTGTCAGACCTACCCGCAAAGCATACAATATTTTGCTCGACGCATTTGCAGTATCAGGAATGGTGGACCAAGCTCGAACTGTTTTTAAAAGCATGAGAAGGGACAGGTATGAACCCGATATATACTCGTATACCACTATGCTGTCAGCTTATGTAAATGCACCCGACATGGAAGGTGCTGAAAAATTCTTCACAAGACTAAAGCAAGATGGATTCAAACCCAATGTCGTCACTTATGGGACCTTAATCAAAGGTTATGCTAAGACAAATGATATTGAGAAGATGATGGAGAAATATGAGGAAATGCAGGCATGTGGTGTCAAACCAAATCAAACGATCTTGACAACCATAATGGATGCGTATGGCAAGAACATTGATTTTGGGAGTGCTGTTGTTTGGTACAAGGAAATGGAATCCTGCAGGCTTACTCCTGATCAGAAAGCAAAGAATATCCTTTTATCTTTGGCAAAAACTGTAGAAGAACAACAGTTAGCAAACCAAATTGTAGGATATTTGGATAATACTAGCAGTGAACAAGGACTTGGTCGTTTATCAATCTCTGTTGAGCAGAATGATGAGGATGAAGACAATTACGAAGATGATGAAGACGATGATGACAGTTATGATGAGGAAGTCGATGGTCCTTCACAGGTCACTTCATCATATGATGATGAACAAGAAAGTGAGCTGGTTTATTTAAATGCTGATAGTGAAAAAAACCTCGATGACTTACTTACAGTGGCTGAATTGTAA

Protein Analysis

454

Amino Acids

51.73

Weight (kDa)

4.73

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 10 - 101 2.5e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 33 - 79 1.8e-08 PPR repeat family
PPR_3 PF13812 34 - 81 5.2e-06 Pentatricopeptide repeat domain
PPR_long PF17177 104 - 227 4.3e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 164 - 220 6.4e-12 Pentatricopeptide repeat domain
TPR_24 PF23276 165 - 269 1.8e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 170 - 201 8.3e-07 PPR repeat
PPR_2 PF13041 173 - 221 3e-12 PPR repeat family
PPR PF01535 176 - 206 1.5e-06 PPR repeat
PPR_2 PF13041 212 - 256 2e-10 PPR repeat family
TPR_24 PF23276 213 - 305 5.3e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 226 - 345 2.1e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 232 - 290 2e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 243 - 292 1.6e-13 PPR repeat family
PPR_3 PF13812 272 - 328 3.1e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 274 - 306 2.4e-06 PPR repeat
PPR_2 PF13041 278 - 315 4.1e-09 PPR repeat family
PPR PF01535 281 - 311 4e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 20, 438
AccI GTMKAC 1 cut(s) 743
AciI CCGC 1 cut(s) 628
AcsI RAATTY 2 cut(s) 44, 797
AcuI CTGAAG 3 cut(s) 174, 183, 288
AdeI CACNNNGTG 1 cut(s) 101
AfaI GTAC 2 cut(s) 488, 1013
AgsI TTSAA 4 cut(s) 49, 79, 458, 829
AjuI GAANNNNNNNTTGG 2 cut(s) 1137, 1169
Alw26I GTCTC 2 cut(s) 275, 515
AlwNI CAGNNNCTG 2 cut(s) 197, 1355
AoxI GGCC 2 cut(s) 145, 194
ApeKI GCWGC 1 cut(s) 249
ApoI RAATTY 2 cut(s) 44, 797
ArsI GACNNNNNNTTYG 4 cut(s) 493, 525, 822, 854
AseI ATTAAT 1 cut(s) 546
AspS9I GGNCC 4 cut(s) 194, 679, 852, 1253
AsuHPI GGTGA 1 cut(s) 297
AsuII TTCGAA 1 cut(s) 175
AvaII GGWCC 3 cut(s) 679, 852, 1253
BbsI GAAGAC 3 cut(s) 180, 1203, 1224
BbvI GCAGC 1 cut(s) 236
BccI CCATC 4 cut(s) 211, 815, 892, 1244
BcgI CGANNNNNNTGC 2 cut(s) 641, 675
BclI TGATCA 1 cut(s) 1045
BcoDI GTCTC 2 cut(s) 275, 515
BfaI CTAG 2 cut(s) 564, 1137
BfmI CTRYAG 2 cut(s) 1030, 1086
BisI GCNGC 1 cut(s) 250
BlsI GCNGC 1 cut(s) 251
Bme18I GGWCC 3 cut(s) 679, 852, 1253
BmgT120I GGNCC 4 cut(s) 194, 679, 852, 1253
BmiI GGNNCC 1 cut(s) 853
BmsI GCATC 4 cut(s) 3, 486, 598, 958
BpiI GAAGAC 3 cut(s) 180, 1203, 1224
BplI GAGNNNNNCTC 2 cut(s) 495, 527
Bpu10I CCTNAGC 1 cut(s) 583
Bpu14I TTCGAA 1 cut(s) 175
BsaI GGTCTC 2 cut(s) 275, 515
BseGI GGATG 3 cut(s) 298, 973, 1198
BseMII CTCAG 2 cut(s) 48, 396
BseRI GAGGAG 1 cut(s) 587
BseXI GCAGC 1 cut(s) 236
BshFI GGCC 2 cut(s) 147, 196
BslFI GGGAC 2 cut(s) 728, 865
BsmAI GTCTC 2 cut(s) 275, 515
BsmFI GGGAC 2 cut(s) 728, 865
BsmI GAATGC 1 cut(s) 186
BsnI GGCC 2 cut(s) 147, 196
Bso31I GGTCTC 2 cut(s) 275, 515
Bsp119I TTCGAA 1 cut(s) 175
Bsp1407I TGTACA 1 cut(s) 486
Bsp143I GATC 3 cut(s) 342, 948, 1045
BspACI CCGC 1 cut(s) 628
BspANI GGCC 2 cut(s) 147, 196
BspCNI CTCAG 2 cut(s) 49, 397
BspLI GGNNCC 1 cut(s) 853
BspMAI CTGCAG 1 cut(s) 1034
BspT104I TTCGAA 1 cut(s) 175
BspTNI GGTCTC 2 cut(s) 275, 515
BsrGI TGTACA 1 cut(s) 486
BssMI GATC 3 cut(s) 342, 948, 1045
BssNAI GTATAC 1 cut(s) 744
Bst1107I GTATAC 1 cut(s) 744
Bst4CI ACNGT 6 cut(s) 430, 694, 1087, 1101, 1232, 1351
Bst6I CTCTTC 1 cut(s) 562
BstAUI TGTACA 1 cut(s) 486
BstBI TTCGAA 1 cut(s) 175
BstC8I GCNNGC 2 cut(s) 921, 1034
BstDEI CTNAG 4 cut(s) 57, 405, 583, 873
BstF5I GGATG 3 cut(s) 298, 973, 1198
BstKTI GATC 3 cut(s) 345, 951, 1048
BstMAI GTCTC 2 cut(s) 275, 515
BstMBI GATC 3 cut(s) 342, 948, 1045
BstNSI RCATGY 1 cut(s) 927
BstSFI CTRYAG 2 cut(s) 1030, 1086
BstV1I GCAGC 1 cut(s) 236
BstV2I GAAGAC 3 cut(s) 180, 1203, 1224
BstZ17I GTATAC 1 cut(s) 744
BsuRI GGCC 2 cut(s) 147, 196
BtsCI GGATG 3 cut(s) 298, 973, 1198
BtsI GCAGTG 2 cut(s) 402, 1147
BtsIMutI CAGTG 3 cut(s) 402, 1147, 1356
Cac8I GCNNGC 2 cut(s) 921, 1034
CaiI CAGNNNCTG 2 cut(s) 197, 1355
Cfr13I GGNCC 4 cut(s) 194, 679, 852, 1253
CseI GACGC 1 cut(s) 662
Csp6I GTAC 2 cut(s) 487, 1012
CviAII CATG 4 cut(s) 337, 706, 781, 924
CviQI GTAC 2 cut(s) 487, 1012
DdeI CTNAG 4 cut(s) 57, 405, 583, 873
DpnI GATC 3 cut(s) 344, 950, 1047
DpnII GATC 3 cut(s) 342, 948, 1045
DraI TTTAAA 3 cut(s) 312, 700, 1311
DraIII CACNNNGTG 1 cut(s) 101
Eam1104I CTCTTC 1 cut(s) 562
EarI CTCTTC 1 cut(s) 562
Eco31I GGTCTC 2 cut(s) 275, 515
Eco47I GGWCC 3 cut(s) 679, 852, 1253
Eco57I CTGAAG 3 cut(s) 174, 183, 288
EcoO109I RGGNCCY 2 cut(s) 194, 852
EcoT22I ATGCAT 1 cut(s) 553
FaeI CATG 4 cut(s) 340, 709, 784, 927
FaqI GGGAC 2 cut(s) 728, 865
FatI CATG 4 cut(s) 336, 705, 780, 923
FauI CCCGC 1 cut(s) 635
FauNDI CATATG 3 cut(s) 133, 553, 1276
FbaI TGATCA 1 cut(s) 1045
FblI GTMKAC 1 cut(s) 743
Fnu4HI GCNGC 1 cut(s) 250
FokI GGATG 3 cut(s) 305, 980, 1205
Fsp4HI GCNGC 1 cut(s) 250
FspBI CTAG 2 cut(s) 564, 1137
GluI GCNGC 1 cut(s) 250
HaeIII GGCC 2 cut(s) 147, 196
HgaI GACGC 1 cut(s) 662
Hin1II CATG 4 cut(s) 340, 709, 784, 927
HindIII AAGCTT 1 cut(s) 70
HinfI GANTC 2 cut(s) 825, 1025
HphI GGTGA 1 cut(s) 297
Hpy166II GTNNAC 3 cut(s) 679, 744, 1145
Hpy188I TCNGA 4 cut(s) 12, 515, 620, 1050
Hpy188III TCNNGA 3 cut(s) 669, 952, 1043
Hpy8I GTNNAC 3 cut(s) 679, 744, 1145
Hpy99I CGWCG 1 cut(s) 656
HpyAV CCTTC 5 cut(s) 55, 352, 705, 779, 1266
HpyCH4III ACNGT 6 cut(s) 430, 694, 1087, 1101, 1232, 1351
HpyF3I CTNAG 4 cut(s) 57, 405, 583, 873
Hsp92II CATG 4 cut(s) 340, 709, 784, 927
Ksp22I TGATCA 1 cut(s) 1045
Kzo9I GATC 3 cut(s) 342, 948, 1045
Lsp1109I GCAGC 1 cut(s) 236
LweI GCATC 4 cut(s) 3, 486, 598, 958
MaeI CTAG 2 cut(s) 564, 1137
MaeIII GTNAC 5 cut(s) 211, 303, 430, 841, 1264
MalI GATC 3 cut(s) 344, 950, 1047
MboI GATC 3 cut(s) 342, 948, 1045
MluCI AATT 6 cut(s) 44, 222, 797, 1113, 1201, 1358
MmeI TCCRAC 1 cut(s) 538
Mph1103I ATGCAT 1 cut(s) 553
MseI TTAA 8 cut(s) 68, 258, 311, 354, 546, 699, 857, 1310
Mva1269I GAATGC 1 cut(s) 186
NdeI CATATG 3 cut(s) 133, 553, 1276
NdeII GATC 3 cut(s) 342, 948, 1045
NlaIII CATG 4 cut(s) 340, 709, 784, 927
NlaIV GGNNCC 1 cut(s) 853
NmuCI GTSAC 4 cut(s) 211, 303, 841, 1264
NsiI ATGCAT 1 cut(s) 553
NspI RCATGY 1 cut(s) 927
NspV TTCGAA 1 cut(s) 175
PctI GAATGC 1 cut(s) 186
PfeI GAWTC 2 cut(s) 825, 1025
PflFI GACNNNGTC 1 cut(s) 1155
PkrI GCNGC 1 cut(s) 251
PpuMI RGGWCCY 1 cut(s) 852
PshBI ATTAAT 1 cut(s) 546
PsiI TTATAA 2 cut(s) 20, 438
Psp5II RGGWCCY 1 cut(s) 852
PspN4I GGNNCC 1 cut(s) 853
PspPI GGNCC 4 cut(s) 194, 679, 852, 1253
PspPPI RGGWCCY 1 cut(s) 852
PstI CTGCAG 1 cut(s) 1034
PstNI CAGNNNCTG 2 cut(s) 197, 1355
PsyI GACNNNGTC 1 cut(s) 1155
RsaI GTAC 2 cut(s) 488, 1013
RsaNI GTAC 2 cut(s) 487, 1012
SaqAI TTAA 8 cut(s) 68, 258, 311, 354, 546, 699, 857, 1310
SatI GCNGC 1 cut(s) 250
Sau3AI GATC 3 cut(s) 342, 948, 1045
Sau96I GGNCC 4 cut(s) 194, 679, 852, 1253
SbfI CCTGCAGG 1 cut(s) 1034
SdaI CCTGCAGG 1 cut(s) 1034
SfaNI GCATC 4 cut(s) 3, 486, 598, 958
SfcI CTRYAG 2 cut(s) 1030, 1086
SfuI TTCGAA 1 cut(s) 175
SinI GGWCC 3 cut(s) 679, 852, 1253
SmiI ATTTAAAT 1 cut(s) 1311
Sse8387I CCTGCAGG 1 cut(s) 1034
Sse9I AATT 6 cut(s) 44, 222, 797, 1113, 1201, 1358
SsiI CCGC 1 cut(s) 628
SspI AATATT 3 cut(s) 171, 237, 643
SspMI CTAG 2 cut(s) 564, 1137
SwaI ATTTAAAT 1 cut(s) 1311
TaaI ACNGT 6 cut(s) 430, 694, 1087, 1101, 1232, 1351
TaqI TCGA 5 cut(s) 175, 651, 688, 1248, 1335
TasI AATT 6 cut(s) 44, 222, 797, 1113, 1201, 1358
TatI WGTACW 1 cut(s) 486
TfiI GAWTC 2 cut(s) 825, 1025
Tru1I TTAA 8 cut(s) 68, 258, 311, 354, 546, 699, 857, 1310
Tru9I TTAA 8 cut(s) 68, 258, 311, 354, 546, 699, 857, 1310
TscAI CASTG 3 cut(s) 402, 1147, 1356
TseFI GTSAC 4 cut(s) 211, 303, 841, 1264
TseI GCWGC 1 cut(s) 249
Tsp45I GTSAC 4 cut(s) 211, 303, 841, 1264
TspDTI ATGAA 7 cut(s) 96, 387, 738, 1209, 1230, 1260, 1299
TspRI CASTG 3 cut(s) 402, 1147, 1356
Tth111I GACNNNGTC 1 cut(s) 1155
VpaK11BI GGWCC 3 cut(s) 679, 852, 1253
VspI ATTAAT 1 cut(s) 546
XapI RAATTY 2 cut(s) 44, 797
XceI RCATGY 1 cut(s) 927
XmiI GTMKAC 1 cut(s) 743
XspI CTAG 2 cut(s) 564, 1137
Zsp2I ATGCAT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.