AT3G61920

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012890
Physical Location & Seq
Reverse (-)
22932454 .. 22933362
909 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G61920.1

Sequence Viewer

Length: 564 bp
ATGGGTAACTGCGTATTCAAAGGCAATGGTGGTTCAAGAAAATTATACGATAAAGATGATTCGTTGATAAAAGTGGTGACTCCAAACGGTGGCGTGATGGAGCTTCATCCTCCCATCTTCGCCGAATTCATCACCAACGAATTTCCCGGCCATGTCATCCACGACTCTTTAAGCCTCCGCCACTCATCTCCACCGCTTCTCCACGGCGAAGAACTCTTTCCCGGTAACATCTACTACCTCCTTCCTCTTTCTTCTTCCGCAGCCGCGACCGCTCAACTGGATTCCTCCGACCAACTATCAACGCCGTACAGAATGTCTTTCGGGAAGACGCCGATAATGGCGGCTTTGAGTGGCGGTGGTTGTGGAGTGTGGAAGGTGAGGCTTGTGATAAGTCCGGAGCAGTTGGCGGAAATTCTTGCGGAGGATGTGGAAACGGAAGCGTTGGTGGAAAGTGTGAGGACGGTGGCGAAGTGTGGCGGTTACGGCTGCGGCGGAGGAGTTCATTCGAGAGCGAATTCAGACCAGCTAAGCGTTACGAGTAGCTTTAAAGGGAAATTGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

187

Amino Acids

19.94

Weight (kDa)

5.83

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 153 2.1e-22 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61920
fragaria_vesca FvH4_7g17560
malus_domestica MD01G1088400.v1.1 MD07G1158800.v1.1 MD08G1186200.v1.1
prunus_persica Prupe.2G196900_v2.0.a1
pyrus_communis pycom01g11210 pycom07g15530
rosa_chinensis RchiOBHm_Chr1g0361011
rosa_laevigata RLG00000027757
rosa_multiflora Rmu_sc0000677.1_g000009
rosa_roxburghii Rroxscaffold_4G00295530
rosa_rugosa Rorug01G0284200
rosa_samantha Rh1AG295800 Rh1BG260500 Rh1CG277700 Rh1DG290500
rosa_wichuraiana Rw1G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 89
AccBSI CCGCTC 1 cut(s) 272
AccII CGCG 1 cut(s) 266
AccIII TCCGGA 1 cut(s) 394
AcoI YGGCCR 1 cut(s) 148
AcsI RAATTY 4 cut(s) 125, 140, 411, 514
AcyI GRCGYC 1 cut(s) 329
AfaI GTAC 1 cut(s) 308
AfiI CCNNNNNNNGG 1 cut(s) 89
AgsI TTSAA 2 cut(s) 19, 36
AluBI AGCT 3 cut(s) 103, 526, 543
AluI AGCT 3 cut(s) 103, 526, 543
Aor13HI TCCGGA 1 cut(s) 394
AoxI GGCC 1 cut(s) 148
ApeKI GCWGC 2 cut(s) 260, 486
ApoI RAATTY 4 cut(s) 125, 140, 411, 514
Asp700I GAANNNNTTC 1 cut(s) 216
AsuC2I CCSGG 2 cut(s) 147, 222
AsuHPI GGTGA 3 cut(s) 88, 124, 388
BbsI GAAGAC 1 cut(s) 332
BbvI GCAGC 2 cut(s) 272, 473
BccI CCATC 2 cut(s) 91, 122
BceAI ACGGC 3 cut(s) 220, 289, 499
BcnI CCSGG 2 cut(s) 147, 222
BisI GCNGC 5 cut(s) 261, 264, 342, 487, 490
BlpI GCTNAGC 1 cut(s) 527
BlsI GCNGC 5 cut(s) 262, 265, 343, 488, 491
Bme1390I CCNGG 2 cut(s) 147, 222
BmrFI CCNGG 2 cut(s) 147, 222
BpiI GAAGAC 1 cut(s) 332
Bpu1102I GCTNAGC 1 cut(s) 527
BpuMI CCSGG 2 cut(s) 147, 222
BsaHI GRCGYC 1 cut(s) 329
BsaJI CCNNGG 1 cut(s) 202
BsaWI WCCGGW 1 cut(s) 394
BsaXI ACNNNNNCTCC 2 cut(s) 183, 213
Bsc4I CCNNNNNNNGG 1 cut(s) 89
Bse1I ACTGG 1 cut(s) 282
Bse3DI GCAATG 1 cut(s) 31
BseAI TCCGGA 1 cut(s) 394
BseDI CCNNGG 1 cut(s) 202
BseGI GGATG 3 cut(s) 106, 156, 430
BseLI CCNNNNNNNGG 1 cut(s) 89
BseMI GCAATG 1 cut(s) 31
BseNI ACTGG 1 cut(s) 282
BseRI GAGGAG 1 cut(s) 510
BseXI GCAGC 2 cut(s) 272, 473
Bsh1236I CGCG 1 cut(s) 266
Bsh1285I CGRYCG 1 cut(s) 270
BshFI GGCC 1 cut(s) 150
BsiEI CGRYCG 1 cut(s) 270
BsiSI CCGG 3 cut(s) 147, 222, 395
BslI CCNNNNNNNGG 1 cut(s) 89
BsnI GGCC 1 cut(s) 150
Bsp13I TCCGGA 1 cut(s) 394
Bsp1720I GCTNAGC 1 cut(s) 527
BspANI GGCC 1 cut(s) 150
BspEI TCCGGA 1 cut(s) 394
BspFNI CGCG 1 cut(s) 266
BsrBI CCGCTC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 31
BsrI ACTGG 1 cut(s) 282
BssECI CCNNGG 1 cut(s) 202
BssNI GRCGYC 1 cut(s) 329
Bst4CI ACNGT 2 cut(s) 89, 463
BstACI GRCGYC 1 cut(s) 329
BstDEI CTNAG 1 cut(s) 527
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 3 cut(s) 106, 156, 430
BstFNI CGCG 1 cut(s) 266
BstMCI CGRYCG 1 cut(s) 270
BstMWI GCNNNNNNNGC 2 cut(s) 269, 483
BstSCI CCNGG 2 cut(s) 145, 220
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 2 cut(s) 272, 473
BstV2I GAAGAC 1 cut(s) 332
BsuRI GGCC 1 cut(s) 150
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 3 cut(s) 106, 156, 430
CseI GACGC 1 cut(s) 337
Csp6I GTAC 1 cut(s) 307
CviAII CATG 1 cut(s) 152
CviJI RGCY 9 cut(s) 103, 150, 174, 263, 344, 382, 486, 526, 543
CviKI_1 RGCY 9 cut(s) 103, 150, 174, 263, 344, 382, 486, 526, 543
CviQI GTAC 1 cut(s) 307
DdeI CTNAG 1 cut(s) 527
DraI TTTAAA 1 cut(s) 547
EaeI YGGCCR 1 cut(s) 148
EciI GGCGGA 3 cut(s) 167, 422, 507
EcoRI GAATTC 2 cut(s) 125, 514
FaeI CATG 1 cut(s) 155
FaiI YATR 2 cut(s) 46, 153
FatI CATG 1 cut(s) 151
Fnu4HI GCNGC 5 cut(s) 261, 264, 342, 487, 490
FokI GGATG 3 cut(s) 93, 143, 437
Fsp4HI GCNGC 5 cut(s) 261, 264, 342, 487, 490
GluI GCNGC 5 cut(s) 261, 264, 342, 487, 490
HaeIII GGCC 1 cut(s) 150
HapII CCGG 3 cut(s) 147, 222, 395
HgaI GACGC 1 cut(s) 337
Hin1I GRCGYC 1 cut(s) 329
Hin1II CATG 1 cut(s) 155
HinfI GANTC 4 cut(s) 59, 79, 164, 281
HpaII CCGG 3 cut(s) 147, 222, 395
HphI GGTGA 3 cut(s) 88, 124, 388
Hpy188I TCNGA 2 cut(s) 289, 520
Hpy188III TCNNGA 4 cut(s) 36, 322, 395, 507
HpyAV CCTTC 2 cut(s) 251, 367
HpyCH4III ACNGT 2 cut(s) 89, 463
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 483
HpyF3I CTNAG 1 cut(s) 527
Hsp92I GRCGYC 1 cut(s) 329
Hsp92II CATG 1 cut(s) 155
Kpn2I TCCGGA 1 cut(s) 394
LmnI GCTCC 2 cut(s) 100, 397
LpnPI CCDG 5 cut(s) 160, 235, 263, 408, 536
Lsp1109I GCAGC 2 cut(s) 272, 473
MaeIII GTNAC 5 cut(s) 5, 76, 224, 479, 532
MbiI CCGCTC 1 cut(s) 272
MboII GAAGA 5 cut(s) 109, 221, 243, 246, 337
MluCI AATT 6 cut(s) 41, 125, 140, 411, 514, 554
MlyI GAGTC 2 cut(s) 73, 158
MmeI TCCRAC 1 cut(s) 312
MnlI CCTC 9 cut(s) 120, 185, 248, 255, 295, 372, 415, 450, 488
MroI TCCGGA 1 cut(s) 394
MroXI GAANNNNTTC 1 cut(s) 216
MseI TTAA 2 cut(s) 170, 546
MspI CCGG 3 cut(s) 147, 222, 395
MspR9I CCNGG 2 cut(s) 147, 222
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 2 cut(s) 269, 483
NciI CCSGG 2 cut(s) 147, 222
NlaIII CATG 1 cut(s) 155
NmuCI GTSAC 1 cut(s) 76
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 2 cut(s) 59, 281
PflMI CCANNNNNTGG 1 cut(s) 89
PkrI GCNGC 5 cut(s) 262, 265, 343, 488, 491
PleI GAGTC 2 cut(s) 73, 158
PpsI GAGTC 2 cut(s) 73, 158
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
SaqAI TTAA 2 cut(s) 170, 546
SatI GCNGC 5 cut(s) 261, 264, 342, 487, 490
SchI GAGTC 2 cut(s) 73, 158
ScrFI CCNGG 2 cut(s) 147, 222
SetI ASST 5 cut(s) 105, 240, 378, 528, 545
Sse9I AATT 6 cut(s) 41, 125, 140, 411, 514, 554
StyD4I CCNGG 2 cut(s) 145, 220
TaaI ACNGT 2 cut(s) 89, 463
TaqI TCGA 1 cut(s) 506
TasI AATT 6 cut(s) 41, 125, 140, 411, 514, 554
TauI GCSGC 3 cut(s) 266, 344, 492
TfiI GAWTC 2 cut(s) 59, 281
Tru1I TTAA 2 cut(s) 170, 546
Tru9I TTAA 2 cut(s) 170, 546
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 2 cut(s) 260, 486
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 3 cut(s) 95, 118, 491
TspGWI ACGGA 1 cut(s) 449
Van91I CCANNNNNTGG 1 cut(s) 89
XapI RAATTY 4 cut(s) 125, 140, 411, 514
XmnI GAANNNNTTC 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.