pycom07g15530

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012890
Physical Location & Seq
Reverse (-)
17989348 .. 17989896
549 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g15530.1

Sequence Viewer

Length: 549 bp
ATGGGAAATTGTGTATTTAAAGGGTTTCGAGAGGCAGAAGAGATGGTGAAAGTCGTGACATGCAGTGGGGGAATAATGGAGCTCTTTGCCCCCATCACAGCTAACTCCATAACCAAAGAGTTCCCAGGCCATGCCATCTTCCGCAGCCCTGACCTCTTCGCACAACCTCTTCTCCACAACGACGAGCTCCATCCCAAAGAGCTTTACTATCTCCTACCTCTCAACCCCTACAAAACCCCCAAAAATCATGATATTAATGCAAACCCTAACGGCACCGTTTGTACACCGTATCGCATGTCATGTGACAATCAAGGCAAGAGGCCGGCAGATCCTGAAGTTTTCCCGAGGTACAACAGTAGTGGGGTTTGGAAGGTGAAGCTGGTGATAAGTCCAGAGCAGCTCTCTGATATTTTGTCGCAGGAAGCTCGCACTGAGGCTTTGATTGAAAGCGTGAGGACTGTGGCCAAGTGTGGCACCGGAGGAAACTCATCGGTGGCAAGTTCCGACCAGTGGAGTGTGTCTAGTAGTTGGAAGGAGCTGTCATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

183

Amino Acids

20.09

Weight (kDa)

6.09

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 171 1.4e-22 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61920
fragaria_vesca FvH4_7g17560
malus_domestica MD01G1088400.v1.1 MD07G1158800.v1.1 MD08G1186200.v1.1
prunus_persica Prupe.2G196900_v2.0.a1
pyrus_communis pycom01g11210 pycom07g15530
rosa_chinensis RchiOBHm_Chr1g0361011
rosa_laevigata RLG00000027757
rosa_multiflora Rmu_sc0000677.1_g000009
rosa_roxburghii Rroxscaffold_4G00295530
rosa_rugosa Rorug01G0284200
rosa_samantha Rh1AG295800 Rh1BG260500 Rh1CG277700 Rh1DG290500
rosa_wichuraiana Rw1G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 272, 473
AciI CCGC 1 cut(s) 142
AclWI GGATC 1 cut(s) 323
AcoI YGGCCR 1 cut(s) 462
AcuI CTGAAG 1 cut(s) 354
AfaI GTAC 2 cut(s) 283, 350
AfiI CCNNNNNNNGG 1 cut(s) 510
AgsI TTSAA 1 cut(s) 446
AjnI CCWGG 1 cut(s) 124
AluBI AGCT 8 cut(s) 82, 101, 187, 202, 379, 400, 425, 538
AluI AGCT 8 cut(s) 82, 101, 187, 202, 379, 400, 425, 538
Alw21I GWGCWC 2 cut(s) 84, 189
AlwI GGATC 1 cut(s) 323
AlwNI CAGNNNCTG 1 cut(s) 332
Ama87I CYCGRG 1 cut(s) 343
AoxI GGCC 3 cut(s) 127, 320, 462
ApeKI GCWGC 2 cut(s) 144, 397
AseI ATTAAT 1 cut(s) 255
AsuHPI GGTGA 3 cut(s) 58, 385, 394
AvaI CYCGRG 1 cut(s) 343
BaeI ACNNNNGTAYC 2 cut(s) 340, 373
BalI TGGCCA 1 cut(s) 464
BanI GGYRCC 2 cut(s) 272, 473
BanII GRGCYC 2 cut(s) 84, 189
Bbv12I GWGCWC 2 cut(s) 84, 189
BbvI GCAGC 2 cut(s) 156, 409
BccI CCATC 4 cut(s) 37, 101, 143, 198
BceAI ACGGC 1 cut(s) 286
BcgI CGANNNNNNTGC 2 cut(s) 407, 441
BciT130I CCWGG 1 cut(s) 126
BfaI CTAG 1 cut(s) 522
BisI GCNGC 2 cut(s) 145, 398
BlsI GCNGC 2 cut(s) 146, 399
Bme1390I CCNGG 1 cut(s) 126
BmeT110I CYCGRG 1 cut(s) 343
BmiI GGNNCC 2 cut(s) 274, 475
BmrFI CCNGG 1 cut(s) 126
BplI GAGNNNNNCTC 2 cut(s) 386, 418
BsaJI CCNNGG 2 cut(s) 124, 344
BsaWI WCCGGW 1 cut(s) 476
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 1 cut(s) 510
Bse118I RCCGGY 1 cut(s) 322
Bse1I ACTGG 1 cut(s) 508
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 2 cut(s) 124, 344
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 1 cut(s) 510
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 1 cut(s) 508
BseXI GCAGC 2 cut(s) 156, 409
BshFI GGCC 3 cut(s) 129, 322, 464
BshNI GGYRCC 2 cut(s) 272, 473
BsiHKAI GWGCWC 2 cut(s) 84, 189
BsiHKCI CYCGRG 1 cut(s) 343
BsiSI CCGG 2 cut(s) 323, 477
BslI CCNNNNNNNGG 1 cut(s) 510
BsnI GGCC 3 cut(s) 129, 322, 464
BsoBI CYCGRG 1 cut(s) 343
Bsp1286I GDGCHC 2 cut(s) 84, 189
Bsp1407I TGTACA 1 cut(s) 281
Bsp143I GATC 1 cut(s) 328
BspACI CCGC 1 cut(s) 142
BspANI GGCC 3 cut(s) 129, 322, 464
BspCNI CTCAG 1 cut(s) 424
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 274, 475
BspPI GGATC 1 cut(s) 323
BspT107I GGYRCC 2 cut(s) 272, 473
BsrFI RCCGGY 1 cut(s) 322
BsrGI TGTACA 1 cut(s) 281
BsrI ACTGG 1 cut(s) 508
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 2 cut(s) 124, 344
BssMI GATC 1 cut(s) 328
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 4 cut(s) 277, 288, 356, 460
Bst6I CTCTTC 3 cut(s) 33, 161, 174
BstAUI TGTACA 1 cut(s) 281
BstC8I GCNNGC 2 cut(s) 324, 427
BstDEI CTNAG 1 cut(s) 432
BstF5I GGATG 1 cut(s) 190
BstKTI GATC 1 cut(s) 331
BstMBI GATC 1 cut(s) 328
BstNI CCWGG 1 cut(s) 126
BstNSI RCATGY 2 cut(s) 63, 298
BstSCI CCNGG 1 cut(s) 124
BstV1I GCAGC 2 cut(s) 156, 409
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BsuRI GGCC 3 cut(s) 129, 322, 464
BtsCI GGATG 1 cut(s) 190
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 3 cut(s) 70, 429, 515
Cac8I GCNNGC 2 cut(s) 324, 427
CaiI CAGNNNCTG 1 cut(s) 332
CciI TCATGA 1 cut(s) 247
Cfr10I RCCGGY 1 cut(s) 322
Csp6I GTAC 2 cut(s) 282, 349
CspCI CAANNNNNGTGG 2 cut(s) 340, 375
CviAII CATG 5 cut(s) 60, 131, 248, 295, 300
CviQI GTAC 2 cut(s) 282, 349
DdeI CTNAG 1 cut(s) 432
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
DraI TTTAAA 1 cut(s) 19
EaeI YGGCCR 1 cut(s) 462
Eam1104I CTCTTC 3 cut(s) 33, 161, 174
EarI CTCTTC 3 cut(s) 33, 161, 174
Ecl136II GAGCTC 2 cut(s) 82, 187
Eco24I GRGCYC 2 cut(s) 84, 189
Eco53kI GAGCTC 2 cut(s) 82, 187
Eco57I CTGAAG 1 cut(s) 354
Eco88I CYCGRG 1 cut(s) 343
EcoICRI GAGCTC 2 cut(s) 82, 187
EcoRII CCWGG 1 cut(s) 124
EcoT38I GRGCYC 2 cut(s) 84, 189
FaeI CATG 5 cut(s) 63, 134, 251, 298, 303
FaiI YATR 6 cut(s) 61, 110, 132, 249, 296, 301
FatI CATG 5 cut(s) 59, 130, 247, 294, 299
Fnu4HI GCNGC 2 cut(s) 145, 398
FokI GGATG 1 cut(s) 177
FriOI GRGCYC 2 cut(s) 84, 189
Fsp4HI GCNGC 2 cut(s) 145, 398
FspBI CTAG 1 cut(s) 522
GluI GCNGC 2 cut(s) 145, 398
HaeIII GGCC 3 cut(s) 129, 322, 464
HapII CCGG 2 cut(s) 323, 477
Hin1II CATG 5 cut(s) 63, 134, 251, 298, 303
HpaII CCGG 2 cut(s) 323, 477
HphI GGTGA 3 cut(s) 58, 385, 394
Hpy166II GTNNAC 1 cut(s) 284
Hpy188I TCNGA 2 cut(s) 406, 505
Hpy188III TCNNGA 7 cut(s) 29, 55, 248, 332, 343, 392, 546
Hpy8I GTNNAC 1 cut(s) 284
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 2 cut(s) 364, 526
HpyCH4III ACNGT 4 cut(s) 277, 288, 356, 460
HpyCH4V TGCA 2 cut(s) 63, 260
HpyF3I CTNAG 1 cut(s) 432
Hsp92II CATG 5 cut(s) 63, 134, 251, 298, 303
KroI GCCGGC 1 cut(s) 322
KroNI GCCGGC 1 cut(s) 324
Kzo9I GATC 1 cut(s) 328
LmnI GCTCC 3 cut(s) 79, 192, 535
Lsp1109I GCAGC 2 cut(s) 156, 409
MaeI CTAG 1 cut(s) 522
MaeIII GTNAC 2 cut(s) 55, 302
MalI GATC 1 cut(s) 330
MboI GATC 1 cut(s) 328
MboII GAAGA 4 cut(s) 50, 130, 148, 161
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 2 cut(s) 84, 189
MlsI TGGCCA 1 cut(s) 464
MluCI AATT 1 cut(s) 7
MluNI TGGCCA 1 cut(s) 464
MmeI TCCRAC 2 cut(s) 509, 528
MnlI CCTC 9 cut(s) 25, 164, 177, 228, 312, 339, 427, 447, 473
Mox20I TGGCCA 1 cut(s) 464
MroNI GCCGGC 1 cut(s) 322
MscI TGGCCA 1 cut(s) 464
MseI TTAA 2 cut(s) 18, 255
Msp20I TGGCCA 1 cut(s) 464
MspI CCGG 2 cut(s) 323, 477
MspR9I CCNGG 1 cut(s) 126
MvaI CCWGG 1 cut(s) 126
NaeI GCCGGC 1 cut(s) 324
NdeII GATC 1 cut(s) 328
NgoMIV GCCGGC 1 cut(s) 322
NlaIII CATG 5 cut(s) 63, 134, 251, 298, 303
NlaIV GGNNCC 2 cut(s) 274, 475
NmuCI GTSAC 2 cut(s) 55, 302
NspI RCATGY 2 cut(s) 63, 298
PagI TCATGA 1 cut(s) 247
PdiI GCCGGC 1 cut(s) 324
PkrI GCNGC 2 cut(s) 146, 399
PshBI ATTAAT 1 cut(s) 255
Psp124BI GAGCTC 2 cut(s) 84, 189
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 2 cut(s) 274, 475
PstNI CAGNNNCTG 1 cut(s) 332
PsuI RGATCY 1 cut(s) 328
RsaI GTAC 2 cut(s) 283, 350
RsaNI GTAC 2 cut(s) 282, 349
SacI GAGCTC 2 cut(s) 84, 189
SaqAI TTAA 2 cut(s) 18, 255
SatI GCNGC 2 cut(s) 145, 398
Sau3AI GATC 1 cut(s) 328
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 2 cut(s) 84, 189
Sse9I AATT 1 cut(s) 7
SsiI CCGC 1 cut(s) 142
SspMI CTAG 1 cut(s) 522
SstI GAGCTC 2 cut(s) 84, 189
StyD4I CCNGG 1 cut(s) 124
TaaI ACNGT 4 cut(s) 277, 288, 356, 460
TaqI TCGA 1 cut(s) 28
TasI AATT 1 cut(s) 7
TatI WGTACW 1 cut(s) 281
Tru1I TTAA 2 cut(s) 18, 255
Tru9I TTAA 2 cut(s) 18, 255
TscAI CASTG 3 cut(s) 70, 436, 515
TseFI GTSAC 2 cut(s) 55, 302
TseI GCWGC 2 cut(s) 144, 397
Tsp45I GTSAC 2 cut(s) 55, 302
TspRI CASTG 3 cut(s) 70, 436, 515
VspI ATTAAT 1 cut(s) 255
XceI RCATGY 2 cut(s) 63, 298
XspI CTAG 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.