MD08G1186200.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012890
Physical Location & Seq
Forward (+)
23446862 .. 23447410
549 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1186200.v1.1.491

Sequence Viewer

Length: 549 bp
ATGGGAAATTGTGTGTTTAAAGAGCTTCGAGAGGCAGAAGAGATGGTGAAAGTCGTGACATACAGTGGGGGAATAATAGAGCTCTTTGCCCCCATCACAACTAACTCTATAACCAATGAGTTCCCAGGCCATGCCATCTTCCACAGCCCTGACCTCTTCGCGCAACTTCCTCTCCACAACGAAGAGCTTCATCCTAAAGAGCTTTACTATCTCCTACCTCTCAACCCCTACAAAACCCCAAAAAATCATGATATTAATGCAAACCCTAACGACATTGTTTGTACACCGTATCGCATGTCATGTGACAATCAAGGCAAGAGGCCGGCAGATCCTGAAGTTTTTCCGAGGTACAACAGTAGTGGGGTTTGGAAGGTGAAGCTGGTGATAAGTCCAGAGCAGCTCTCTGACATTTTGTCGCAGGAAGCTCGCACTGAGGCTTTGATTAAAAGCATGAGGATAGTGGCCAAGTGTGGCACCGGAAGGAACTCATCGGTGGCAAGTTCCGATCAGTGGAGTCTGTCTAGTAGGTGGAATGAGCTATCATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

183

Amino Acids

20.47

Weight (kDa)

5.95

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 172 1.5e-18 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61920
fragaria_vesca FvH4_7g17560
malus_domestica MD01G1088400.v1.1 MD07G1158800.v1.1 MD08G1186200.v1.1
prunus_persica Prupe.2G196900_v2.0.a1
pyrus_communis pycom01g11210 pycom07g15530
rosa_chinensis RchiOBHm_Chr1g0361011
rosa_laevigata RLG00000027757
rosa_multiflora Rmu_sc0000677.1_g000009
rosa_roxburghii Rroxscaffold_4G00295530
rosa_rugosa Rorug01G0284200
rosa_samantha Rh1AG295800 Rh1BG260500 Rh1CG277700 Rh1DG290500
rosa_wichuraiana Rw1G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 473
AccII CGCG 1 cut(s) 161
AclWI GGATC 1 cut(s) 323
AcoI YGGCCR 1 cut(s) 462
AcuI CTGAAG 1 cut(s) 354
AfaI GTAC 2 cut(s) 283, 350
AfiI CCNNNNNNNGG 1 cut(s) 510
AhdI GACNNNNNGTC 1 cut(s) 412
AjnI CCWGG 1 cut(s) 124
AluBI AGCT 8 cut(s) 25, 82, 187, 202, 379, 400, 425, 538
AluI AGCT 8 cut(s) 25, 82, 187, 202, 379, 400, 425, 538
Alw21I GWGCWC 1 cut(s) 84
AlwI GGATC 1 cut(s) 323
AlwNI CAGNNNCTG 1 cut(s) 332
AoxI GGCC 3 cut(s) 127, 320, 462
ApeKI GCWGC 1 cut(s) 397
AseI ATTAAT 1 cut(s) 255
Asp700I GAANNNNTTC 2 cut(s) 186, 339
AspLEI GCGC 1 cut(s) 163
AsuHPI GGTGA 3 cut(s) 58, 385, 394
BaeI ACNNNNGTAYC 2 cut(s) 340, 373
BalI TGGCCA 1 cut(s) 464
BanI GGYRCC 1 cut(s) 473
BanII GRGCYC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 409
BccI CCATC 3 cut(s) 37, 101, 143
BcgI CGANNNNNNTGC 2 cut(s) 407, 441
BciT130I CCWGG 1 cut(s) 126
BfaI CTAG 1 cut(s) 522
BisI GCNGC 1 cut(s) 398
BlsI GCNGC 1 cut(s) 399
Bme1390I CCNGG 1 cut(s) 126
BmeRI GACNNNNNGTC 1 cut(s) 412
BmiI GGNNCC 1 cut(s) 475
BmrFI CCNGG 1 cut(s) 126
BplI GAGNNNNNCTC 2 cut(s) 386, 418
BsaJI CCNNGG 2 cut(s) 124, 344
BsaWI WCCGGW 1 cut(s) 476
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 1 cut(s) 510
Bse118I RCCGGY 1 cut(s) 322
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 2 cut(s) 124, 344
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 1 cut(s) 510
BseMII CTCAG 1 cut(s) 423
BseXI GCAGC 1 cut(s) 409
Bsh1236I CGCG 1 cut(s) 161
BshFI GGCC 3 cut(s) 129, 322, 464
BshNI GGYRCC 1 cut(s) 473
BsiHKAI GWGCWC 1 cut(s) 84
BsiSI CCGG 2 cut(s) 323, 477
BslI CCNNNNNNNGG 1 cut(s) 510
BsnI GGCC 3 cut(s) 129, 322, 464
Bsp1286I GDGCHC 1 cut(s) 84
Bsp1407I TGTACA 1 cut(s) 281
Bsp143I GATC 2 cut(s) 328, 505
BspANI GGCC 3 cut(s) 129, 322, 464
BspCNI CTCAG 1 cut(s) 424
BspFNI CGCG 1 cut(s) 161
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 1 cut(s) 475
BspPI GGATC 1 cut(s) 323
BspQI GCTCTTC 1 cut(s) 177
BspT107I GGYRCC 1 cut(s) 473
BsrFI RCCGGY 1 cut(s) 322
BsrGI TGTACA 1 cut(s) 281
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 2 cut(s) 124, 344
BssMI GATC 2 cut(s) 328, 505
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 3 cut(s) 65, 288, 356
Bst6I CTCTTC 3 cut(s) 33, 161, 177
BstAUI TGTACA 1 cut(s) 281
BstC8I GCNNGC 2 cut(s) 324, 427
BstDEI CTNAG 1 cut(s) 432
BstF5I GGATG 1 cut(s) 190
BstFNI CGCG 1 cut(s) 161
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 2 cut(s) 331, 508
BstMBI GATC 2 cut(s) 328, 505
BstNI CCWGG 1 cut(s) 126
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 124
BstUI CGCG 1 cut(s) 161
BstV1I GCAGC 1 cut(s) 409
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BsuRI GGCC 3 cut(s) 129, 322, 464
BtsCI GGATG 1 cut(s) 190
BtsIMutI CAGTG 3 cut(s) 70, 429, 515
Cac8I GCNNGC 2 cut(s) 324, 427
CaiI CAGNNNCTG 1 cut(s) 332
CciI TCATGA 1 cut(s) 247
CfoI GCGC 1 cut(s) 163
Cfr10I RCCGGY 1 cut(s) 322
Csp6I GTAC 2 cut(s) 282, 349
CspCI CAANNNNNGTGG 2 cut(s) 340, 375
CviAII CATG 5 cut(s) 131, 248, 295, 300, 451
CviQI GTAC 2 cut(s) 282, 349
DdeI CTNAG 1 cut(s) 432
DpnI GATC 2 cut(s) 330, 507
DpnII GATC 2 cut(s) 328, 505
DraI TTTAAA 1 cut(s) 19
DriI GACNNNNNGTC 1 cut(s) 412
EaeI YGGCCR 1 cut(s) 462
Eam1104I CTCTTC 3 cut(s) 33, 161, 177
Eam1105I GACNNNNNGTC 1 cut(s) 412
EarI CTCTTC 3 cut(s) 33, 161, 177
Ecl136II GAGCTC 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 84
Eco53kI GAGCTC 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 354
EcoICRI GAGCTC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 124
EcoT38I GRGCYC 1 cut(s) 84
FaeI CATG 5 cut(s) 134, 251, 298, 303, 454
FaiI YATR 7 cut(s) 61, 110, 132, 249, 296, 301, 452
FatI CATG 5 cut(s) 130, 247, 294, 299, 450
Fnu4HI GCNGC 1 cut(s) 398
FokI GGATG 1 cut(s) 177
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 1 cut(s) 398
FspBI CTAG 1 cut(s) 522
GlaI GCGC 1 cut(s) 162
GluI GCNGC 1 cut(s) 398
HaeIII GGCC 3 cut(s) 129, 322, 464
HapII CCGG 2 cut(s) 323, 477
HhaI GCGC 1 cut(s) 163
Hin1II CATG 5 cut(s) 134, 251, 298, 303, 454
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HinfI GANTC 1 cut(s) 514
HpaII CCGG 2 cut(s) 323, 477
HphI GGTGA 3 cut(s) 58, 385, 394
Hpy166II GTNNAC 1 cut(s) 284
Hpy188I TCNGA 3 cut(s) 345, 406, 505
Hpy188III TCNNGA 6 cut(s) 29, 55, 248, 332, 392, 546
Hpy8I GTNNAC 1 cut(s) 284
HpyAV CCTTC 2 cut(s) 364, 474
HpyCH4III ACNGT 3 cut(s) 65, 288, 356
HpyCH4V TGCA 1 cut(s) 260
HpyF3I CTNAG 1 cut(s) 432
Hsp92II CATG 5 cut(s) 134, 251, 298, 303, 454
HspAI GCGC 1 cut(s) 161
KroI GCCGGC 1 cut(s) 322
KroNI GCCGGC 1 cut(s) 324
Kzo9I GATC 2 cut(s) 328, 505
LguI GCTCTTC 1 cut(s) 177
LpnPI CCDG 9 cut(s) 111, 138, 162, 336, 345, 365, 404, 405, 490
Lsp1109I GCAGC 1 cut(s) 409
MaeI CTAG 1 cut(s) 522
MaeIII GTNAC 2 cut(s) 55, 302
MalI GATC 2 cut(s) 330, 507
MboI GATC 2 cut(s) 328, 505
MboII GAAGA 4 cut(s) 50, 130, 148, 194
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 1 cut(s) 84
MlsI TGGCCA 1 cut(s) 464
MluCI AATT 1 cut(s) 7
MluNI TGGCCA 1 cut(s) 464
MlyI GAGTC 1 cut(s) 523
MnlI CCTC 8 cut(s) 25, 164, 180, 228, 312, 339, 427, 447
Mox20I TGGCCA 1 cut(s) 464
MroNI GCCGGC 1 cut(s) 322
MroXI GAANNNNTTC 2 cut(s) 186, 339
MscI TGGCCA 1 cut(s) 464
MseI TTAA 3 cut(s) 18, 255, 444
Msp20I TGGCCA 1 cut(s) 464
MspI CCGG 2 cut(s) 323, 477
MspR9I CCNGG 1 cut(s) 126
MvaI CCWGG 1 cut(s) 126
MvnI CGCG 1 cut(s) 161
NaeI GCCGGC 1 cut(s) 324
NdeII GATC 2 cut(s) 328, 505
NgoMIV GCCGGC 1 cut(s) 322
NlaIII CATG 5 cut(s) 134, 251, 298, 303, 454
NlaIV GGNNCC 1 cut(s) 475
NmuCI GTSAC 2 cut(s) 55, 302
NspI RCATGY 1 cut(s) 298
PagI TCATGA 1 cut(s) 247
PciSI GCTCTTC 1 cut(s) 177
PdiI GCCGGC 1 cut(s) 324
PdmI GAANNNNTTC 2 cut(s) 186, 339
PkrI GCNGC 1 cut(s) 399
PleI GAGTC 1 cut(s) 522
PpsI GAGTC 1 cut(s) 522
PshBI ATTAAT 1 cut(s) 255
Psp124BI GAGCTC 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 475
PstNI CAGNNNCTG 1 cut(s) 332
PsuI RGATCY 1 cut(s) 328
RsaI GTAC 2 cut(s) 283, 350
RsaNI GTAC 2 cut(s) 282, 349
SacI GAGCTC 1 cut(s) 84
SapI GCTCTTC 1 cut(s) 177
SaqAI TTAA 3 cut(s) 18, 255, 444
SatI GCNGC 1 cut(s) 398
Sau3AI GATC 2 cut(s) 328, 505
SchI GAGTC 1 cut(s) 523
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 1 cut(s) 84
Sse9I AATT 1 cut(s) 7
SspMI CTAG 1 cut(s) 522
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 124
TaaI ACNGT 3 cut(s) 65, 288, 356
TaqI TCGA 1 cut(s) 28
TasI AATT 1 cut(s) 7
TatI WGTACW 1 cut(s) 281
Tru1I TTAA 3 cut(s) 18, 255, 444
Tru9I TTAA 3 cut(s) 18, 255, 444
TscAI CASTG 3 cut(s) 70, 436, 515
TseFI GTSAC 2 cut(s) 55, 302
TseI GCWGC 1 cut(s) 397
Tsp45I GTSAC 2 cut(s) 55, 302
TspDTI ATGAA 1 cut(s) 179
TspRI CASTG 3 cut(s) 70, 436, 515
VspI ATTAAT 1 cut(s) 255
XceI RCATGY 1 cut(s) 298
XmnI GAANNNNTTC 2 cut(s) 186, 339
XspI CTAG 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.