Prupe.2G196900_v2.0.a1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0012890
Physical Location & Seq
Reverse (-)
23423747 .. 23424314
568 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G196900.1

Sequence Viewer

Length: 561 bp
ATGGGAAATTGTGTGTTCAAAGGGTTTGGAGAGGCAGAGGAGATGGTGAAGGTGGTGACTTCCAGCGGGGGAATAATGGAGCTCTATGCCCCAATCACAGCCAACGCCATAACCACCGAGTTCCCAGGCCACGCCATCTTCAAATCCCGCGACCTCTTCGCCCAACCTCTCCTCCACAACGAAGAGCTTCATCCCAAAGAGCTCTACTATCTCCTCCCTCTCAACCCCTACAAGTCCACCCCGAAAGACCACGAAAACATCAACGTAAACCCCAACGGCGCCGTTTCTACGCCGTACCGCATGTCGTGCGACAGCCAAGGGTTGCTTAGGAAGAGGGCAGACCCCGAAGTGTTTCCGAGGTACAATAGTAGCGGGGTTTGGAAGGTGAAGCTTGTAATAAGCCCGGAGCAGCTGTCGGAGATTTTGTCGCAGGAGGCTCGCACTGAGGCTTTGATTGAGAGTGTGAGGACGGTGGCCAAGTGCGGCACCGGAGGGAACTCGTCGGCGGCGGGTTCGGATCAGTGGAGTGTGTCTAGCAGTTGGAAGGAGTCGGCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

187

Amino Acids

20.37

Weight (kDa)

5.83

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61920
fragaria_vesca FvH4_7g17560
malus_domestica MD01G1088400.v1.1 MD07G1158800.v1.1 MD08G1186200.v1.1
prunus_persica Prupe.2G196900_v2.0.a1
pyrus_communis pycom01g11210 pycom07g15530
rosa_chinensis RchiOBHm_Chr1g0361011
rosa_laevigata RLG00000027757
rosa_multiflora Rmu_sc0000677.1_g000009
rosa_roxburghii Rroxscaffold_4G00295530
rosa_rugosa Rorug01G0284200
rosa_samantha Rh1AG295800 Rh1BG260500 Rh1CG277700 Rh1DG290500
rosa_wichuraiana Rw1G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 278, 485
AccII CGCG 1 cut(s) 150
AciI CCGC 7 cut(s) 66, 148, 298, 372, 483, 506, 509
AclWI GGATC 1 cut(s) 525
AcoI YGGCCR 1 cut(s) 474
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 2 cut(s) 296, 362
AgsI TTSAA 2 cut(s) 19, 142
AjnI CCWGG 1 cut(s) 124
AluBI AGCT 5 cut(s) 82, 187, 202, 391, 412
AluI AGCT 5 cut(s) 82, 187, 202, 391, 412
Alw21I GWGCWC 2 cut(s) 84, 204
AlwI GGATC 1 cut(s) 525
AoxI GGCC 2 cut(s) 127, 474
ApeKI GCWGC 1 cut(s) 409
Asp700I GAANNNNTTC 2 cut(s) 186, 351
AspLEI GCGC 1 cut(s) 281
AsuC2I CCSGG 1 cut(s) 404
AsuHPI GGTGA 3 cut(s) 58, 67, 397
BaeI ACNNNNGTAYC 2 cut(s) 352, 385
BalI TGGCCA 1 cut(s) 476
BanI GGYRCC 2 cut(s) 278, 485
BanII GRGCYC 2 cut(s) 84, 204
Bbv12I GWGCWC 2 cut(s) 84, 204
BbvI GCAGC 1 cut(s) 421
BccI CCATC 2 cut(s) 37, 143
BceAI ACGGC 3 cut(s) 266, 277, 292
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 1 cut(s) 126
BcnI CCSGG 1 cut(s) 404
BfaI CTAG 1 cut(s) 534
BfoI RGCGCY 1 cut(s) 282
BisI GCNGC 3 cut(s) 410, 484, 507
BlsI GCNGC 3 cut(s) 411, 485, 508
Bme1390I CCNGG 2 cut(s) 126, 404
BmiI GGNNCC 2 cut(s) 280, 487
BmrFI CCNGG 2 cut(s) 126, 404
Bpu10I CCTNAGC 1 cut(s) 326
BpuMI CCSGG 1 cut(s) 404
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 3 cut(s) 124, 316, 356
BsaWI WCCGGW 1 cut(s) 488
BsaXI ACNNNNNCTCC 6 cut(s) 156, 186, 398, 410, 428, 440
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 3 cut(s) 124, 316, 356
BseGI GGATG 1 cut(s) 190
BseMII CTCAG 1 cut(s) 435
BseRI GAGGAG 3 cut(s) 53, 161, 203
BseXI GCAGC 1 cut(s) 421
Bsh1236I CGCG 1 cut(s) 150
BshFI GGCC 2 cut(s) 129, 476
BshNI GGYRCC 2 cut(s) 278, 485
BsiHKAI GWGCWC 2 cut(s) 84, 204
BsiSI CCGG 2 cut(s) 404, 489
BsnI GGCC 2 cut(s) 129, 476
Bsp1286I GDGCHC 2 cut(s) 84, 204
Bsp143I GATC 1 cut(s) 517
BspACI CCGC 7 cut(s) 66, 148, 298, 372, 483, 506, 509
BspANI GGCC 2 cut(s) 129, 476
BspCNI CTCAG 1 cut(s) 436
BspFNI CGCG 1 cut(s) 150
BspHI TCATGA 1 cut(s) 557
BspLI GGNNCC 2 cut(s) 280, 487
BspPI GGATC 1 cut(s) 525
BspQI GCTCTTC 1 cut(s) 177
BspT107I GGYRCC 2 cut(s) 278, 485
BssECI CCNNGG 3 cut(s) 124, 316, 356
BssMI GATC 1 cut(s) 517
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 1 cut(s) 316
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 472
Bst6I CTCTTC 3 cut(s) 161, 177, 326
BstACI GRCGYC 1 cut(s) 279
BstAPI GCANNNNNTGC 1 cut(s) 306
BstC8I GCNNGC 1 cut(s) 439
BstDEI CTNAG 2 cut(s) 326, 444
BstF5I GGATG 1 cut(s) 190
BstFNI CGCG 1 cut(s) 150
BstH2I RGCGCY 1 cut(s) 282
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 1 cut(s) 520
BstMBI GATC 1 cut(s) 517
BstMWI GCNNNNNNNGC 1 cut(s) 306
BstNI CCWGG 1 cut(s) 126
BstNSI RCATGY 1 cut(s) 304
BstSCI CCNGG 2 cut(s) 124, 402
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 1 cut(s) 421
BsuRI GGCC 2 cut(s) 129, 476
BtsCI GGATG 1 cut(s) 190
BtsIMutI CAGTG 2 cut(s) 441, 527
Cac8I GCNNGC 1 cut(s) 439
CciI TCATGA 1 cut(s) 557
CfoI GCGC 1 cut(s) 281
Csp6I GTAC 2 cut(s) 295, 361
CviAII CATG 2 cut(s) 301, 558
CviQI GTAC 2 cut(s) 295, 361
DdeI CTNAG 2 cut(s) 326, 444
DinI GGCGCC 1 cut(s) 280
DpnI GATC 1 cut(s) 519
DpnII GATC 1 cut(s) 517
EaeI YGGCCR 1 cut(s) 474
Eam1104I CTCTTC 3 cut(s) 161, 177, 326
EarI CTCTTC 3 cut(s) 161, 177, 326
Ecl136II GAGCTC 2 cut(s) 82, 202
Eco130I CCWWGG 1 cut(s) 316
Eco24I GRGCYC 2 cut(s) 84, 204
Eco53kI GAGCTC 2 cut(s) 82, 202
EcoICRI GAGCTC 2 cut(s) 82, 202
EcoRII CCWGG 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 316
EcoT38I GRGCYC 2 cut(s) 84, 204
EgeI GGCGCC 1 cut(s) 280
EheI GGCGCC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 2 cut(s) 304, 561
FaiI YATR 4 cut(s) 87, 110, 302, 559
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FatI CATG 2 cut(s) 300, 557
FauI CCCGC 4 cut(s) 59, 155, 365, 502
Fnu4HI GCNGC 3 cut(s) 410, 484, 507
FokI GGATG 1 cut(s) 177
FriOI GRGCYC 2 cut(s) 84, 204
Fsp4HI GCNGC 3 cut(s) 410, 484, 507
FspBI CTAG 1 cut(s) 534
GlaI GCGC 1 cut(s) 280
GluI GCNGC 3 cut(s) 410, 484, 507
HaeII RGCGCY 1 cut(s) 282
HaeIII GGCC 2 cut(s) 129, 476
HapII CCGG 2 cut(s) 404, 489
HhaI GCGC 1 cut(s) 281
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 2 cut(s) 304, 561
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 1 cut(s) 548
HpaII CCGG 2 cut(s) 404, 489
HphI GGTGA 3 cut(s) 58, 67, 397
Hpy166II GTNNAC 2 cut(s) 237, 268
Hpy188I TCNGA 3 cut(s) 357, 418, 517
Hpy188III TCNNGA 1 cut(s) 558
Hpy8I GTNNAC 2 cut(s) 237, 268
Hpy99I CGWCG 1 cut(s) 505
HpyAV CCTTC 3 cut(s) 43, 376, 538
HpyCH4III ACNGT 1 cut(s) 472
HpyCH4IV ACGT 1 cut(s) 264
HpyF10VI GCNNNNNNNGC 1 cut(s) 306
HpyF3I CTNAG 2 cut(s) 326, 444
HpySE526I ACGT 1 cut(s) 264
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 2 cut(s) 304, 561
HspAI GCGC 1 cut(s) 279
KasI GGCGCC 1 cut(s) 278
Kzo9I GATC 1 cut(s) 517
LguI GCTCTTC 1 cut(s) 177
LmnI GCTCC 2 cut(s) 79, 406
LpnPI CCDG 6 cut(s) 76, 111, 138, 416, 417, 502
Lsp1109I GCAGC 1 cut(s) 421
MaeI CTAG 1 cut(s) 534
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 1 cut(s) 55
MalI GATC 1 cut(s) 519
MboI GATC 1 cut(s) 517
MboII GAAGA 4 cut(s) 130, 148, 194, 343
MhlI GDGCHC 2 cut(s) 84, 204
MlsI TGGCCA 1 cut(s) 476
MluCI AATT 1 cut(s) 7
MluNI TGGCCA 1 cut(s) 476
Mly113I GGCGCC 1 cut(s) 279
MlyI GAGTC 1 cut(s) 557
MmeI TCCRAC 2 cut(s) 396, 521
Mox20I TGGCCA 1 cut(s) 476
MroXI GAANNNNTTC 2 cut(s) 186, 351
MscI TGGCCA 1 cut(s) 476
Msp20I TGGCCA 1 cut(s) 476
MspA1I CMGCKG 2 cut(s) 66, 412
MspI CCGG 2 cut(s) 404, 489
MspR9I CCNGG 2 cut(s) 126, 404
MvaI CCWGG 1 cut(s) 126
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 1 cut(s) 306
NarI GGCGCC 1 cut(s) 279
NciI CCSGG 1 cut(s) 404
NdeII GATC 1 cut(s) 517
NlaIII CATG 2 cut(s) 304, 561
NlaIV GGNNCC 2 cut(s) 280, 487
NmuCI GTSAC 1 cut(s) 55
NspI RCATGY 1 cut(s) 304
PagI TCATGA 1 cut(s) 557
PciSI GCTCTTC 1 cut(s) 177
PdmI GAANNNNTTC 2 cut(s) 186, 351
PkrI GCNGC 3 cut(s) 411, 485, 508
PleI GAGTC 1 cut(s) 556
PluTI GGCGCC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 556
Psp124BI GAGCTC 2 cut(s) 84, 204
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 2 cut(s) 280, 487
PvuII CAGCTG 1 cut(s) 412
RsaI GTAC 2 cut(s) 296, 362
RsaNI GTAC 2 cut(s) 295, 361
SacI GAGCTC 2 cut(s) 84, 204
SapI GCTCTTC 1 cut(s) 177
SatI GCNGC 3 cut(s) 410, 484, 507
Sau3AI GATC 1 cut(s) 517
SchI GAGTC 1 cut(s) 557
ScrFI CCNGG 2 cut(s) 126, 404
SduI GDGCHC 2 cut(s) 84, 204
SfoI GGCGCC 1 cut(s) 280
Sse9I AATT 1 cut(s) 7
SsiI CCGC 7 cut(s) 66, 148, 298, 372, 483, 506, 509
SspDI GGCGCC 1 cut(s) 278
SspMI CTAG 1 cut(s) 534
SstI GAGCTC 2 cut(s) 84, 204
StyD4I CCNGG 2 cut(s) 124, 402
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 1 cut(s) 472
TaiI ACGT 1 cut(s) 267
TasI AATT 1 cut(s) 7
TauI GCSGC 2 cut(s) 486, 509
TscAI CASTG 2 cut(s) 448, 527
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 1 cut(s) 179
TspRI CASTG 2 cut(s) 448, 527
XceI RCATGY 1 cut(s) 304
XmnI GAANNNNTTC 2 cut(s) 186, 351
XspI CTAG 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.