Rorug01G0284200

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0012890
Physical Location & Seq
Reverse (-)
39802617 .. 39805770
3154 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0284200.1

Sequence Viewer

Length: 3024 bp
ATGTCCATTTTCTGTTGTAGCTCCATGCAATTGAACCATCCGAGACTCGAAGGGAATGAGACGGATAGGCTGGCATTGCTAGCAATCAAAGCTCAAATACATCACGATCCGAACCGGGTCACAAGCTCATGGAATGAAACCCTTCACTTTTGCCTGTGGCACGGCGTCACCTGCAGTCGACGACACCGCCAAAGGGTGACGAAGCTGGACTTGGGCTCTCTAGCTTTGGGGGGATCCATATCTCCACACATAGGAAATCTAAGCTTCTTAAGAGAGCTAGATCTCAAGAACAATAGCTTCACTCATCAAATCCCTCCTCAAATTGGGCATTTGCATAGATTGCAGGTATTGCTTCTAAACAACAACTCACTTACTGGCACCATTCCTGCCAATATATCCAATTGCTTCAAACTCATCACTCTCAACCTTGGTAAAAACAATCTAGTGGGTAAAATTCCTCCCAAACTAAGTTCCTTGTCAAAGCTTCAGTTTTTTTCTTTGCAAGTCAACAATTTAACAGGAGAGATCCCTCCTTCTTTGGGGAACCTCTCGTCTCTCGAGACATTTTCTGTAGCTATTAATAACTTAGAAGGAAGCCTTCCTAGTTCTCTATGCCAGTGGGAAAGATTAACACGTATCTATTCCTCGTTAAATAGGTTATCTGGTATCATCCCTTCCTGCATTTACAACCTCTCTGGTATAGTTCTTTTCGACGTAGGAGGAAACCAAATTCAAGGAAGTCTTCCATCAAACTTGAGCAATGCTTTTCCCAACCTTCAAATCTTTTCCGTTTTCGACAATCAATTTACTGGGGCCATCCCTTCCTCAATATCCAAGGCAACAAATCTTGTGTCGTTTCAATGTTCGTTAAACAAACTCTCTGGACAGGTGCCAAATTTACGAAATCTTCATAACCTTGGACTATTCATTGTCGGTACTAATAATCTTGGAAGCGGTAAAGATGGTGACTTGAGTTTTGTCTCAGAATTGATCAATGCCACACAGCTTACTGAATTGGACTTAAGACAAAACAATTTTGGAGGCACGTTGCCGACATCAATATCCAATCTCTCAATCAATCTCCAAATCCTTGCAATTGGAGAAAACCACCTACATGGAAGCATCCCAACTGGATTGGGGAATTTTGTCAACCTGCAGTCACTCAGTATGGGAGGAAACTCCTTCACAGGTAGCATCCCCACAGACATTGGGCAGCTTTCAGGGCTTGGGCGGTTGTTTCTTAACAACAACCAATTATCAGGGAGCATTCCATCTTCTCTTGGAAATTTAACACAGTTAACACTTCTCCAATTGCAAGAAAATATTCTAGAGGGTAGCATCCCATCCAGCCTAGGGAAATGCCATTGGTTAAACGAATTGGGTCTTTCCGGAAACAATCTGAATGGCACGATACCGCCCCAAGTTATTGGTCTCTCATCTTTATCAACAATTTTGGGATTGGATAGAAACCAATTGAGTGGTTCCCTTCCCATGGAGATTGGAAATTTGAAGAATCTACGTGGACTACACGTTTTTAATAACTTGTTATCAGGAGAACTTCCCAGTAGCCTCGGCAGTTGCCAGAGCTTAGAAATCCTAGGCTTGCAAGGTAACTTTTTCATGGGACCCATCCCTTCATCTATGAAGGAACTTAGAGGGATTCGAGTTTTAGACCTTTCTCAAAACAATTTGTCAGGAGAAATTCCACAATTTCTTGAGAATCTGAACTTGGAGTACCTGAACCTATCCTTCAACCAGTTCTGGGGAGCAGTACCAACTGGCGGTGTTTTTAAGAATGCAAGTGCGACTTCAGTTGCTGGAAATGCTAGACTCTGCGGAGGTATGGCTATTCTTCGACTTCCTATGTGCAAGCTTAATGAGTCTAAAGGAGAAGGGTTGTCTCGTAGAATGAAACTAATGATCTCTTTAGTCTCCGGGTTCACTCTCTTGGGACTGGTTGTGGTGCTATCTCTTTTTCTTCTTGGTAAGAAAAGGAAAGAAACGAATTTAAGCATTTTGGGGAACTCTGTTTTGCAAGTTTCATATGCTACACTCCTCAGAGCTACTGATGGTTTTTCTTCAGCTAATTTGATCGGTGTGGGTGCTTTTGGGTCTGTGTACAAAGGAACTCTTGGTGATGATAAAGTTGTTGTCGCTGTGAAGGTGCTTAACATGTTACACCGGGGAGCAGCTAAGAGTTTCATGGCCGAATGTGAGGCATTGAGAAATATCAGACATCGAAATTTGGTCAAGATCTTAACTGCATGTTCAAGTATTGATTTTGGCGGCAATGATTTCAAGGCTCTTGTTTACGAGTTCATGGACAACGGGAGCTTGGAAGATTGGTTGCATCCATCTAATGGAACTAGAGAGGTAACAGAAGCACCCAAAAGTTTAAGTCTTATTCAGAGGCTCAACATTGCCATTGATGTTGCTAGTGCATTGGATTATCTTCACAACCATTGTGAAACACCGATCGTTCATTGTGATCTTAAGCCAAGTAATGTTCTCTTGGATGGTGACCTCACTGGGCATGTTTCTGACTTTGGGCTGTCAAGGTTTCTCTCAAAACCAACCATGAGTGTTTCTGGAAATCAATCAAGTTCCATTGGAATTAGAGGATCTGTTGGTTATGCTGCACCAGAGTATGGTATGGGAAGTGAGGTATCCACATACGGGGATGTCTACAGCTTTGGCATTCTCTTGTTAGAAATGTTTACGGGGAAGCGACCCACTGATCACATGTTTAGTGACAGCTTGAATCTTCATAATTATGTGAAGACAGCCCTCCCTGAACTAGTTTCAGAGATTTCAGAATCACTTGTTCTTCAAGAAGGCACCACCAATGTCACTGAAGCTCACAGGCACAGCCGATTGAGTGTGAGAGCACAGAAAATTGAGGAGTGCTTGACTCTGATACTTGGTATAGGAATTGCATGTTCTGTTGAATCCTCCACAAACAGAAAGGATATCAGTGATGTTGCATCTGAATTGCAATCCATTAGGCGCAATCTTCTCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

1007

Amino Acids

108.74

Weight (kDa)

7.59

Isoelectric Point (pI)

35.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 20 - 59 2.1e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 75 - 208 7e-12 Leucine-rich repeat region
LRR_14 PF23598 371 - 462 2.7e-06 Leucine-rich repeat region
Pkinase PF00069 694 - 927 2.3e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 696 - 918 1.2e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61920
fragaria_vesca FvH4_7g17560
malus_domestica MD01G1088400.v1.1 MD07G1158800.v1.1 MD08G1186200.v1.1
prunus_persica Prupe.2G196900_v2.0.a1
pyrus_communis pycom01g11210 pycom07g15530
rosa_chinensis RchiOBHm_Chr1g0361011
rosa_laevigata RLG00000027757
rosa_multiflora Rmu_sc0000677.1_g000009
rosa_roxburghii Rroxscaffold_4G00295530
rosa_rugosa Rorug01G0284200
rosa_samantha Rh1AG295800 Rh1BG260500 Rh1CG277700 Rh1DG290500
rosa_wichuraiana Rw1G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 179
Acc36I ACCTGC 3 cut(s) 179, 334, 1161
AccB1I GGYRCC 3 cut(s) 377, 889, 2837
AccB7I CCANNNNNTGG 2 cut(s) 2360, 2648
AccI GTMKAC 2 cut(s) 178, 2685
AccIII TCCGGA 1 cut(s) 1388
AciI CCGC 7 cut(s) 187, 954, 1231, 1415, 1782, 1836, 2286
AclWI GGATC 5 cut(s) 101, 228, 241, 520, 2629
AcoI YGGCCR 1 cut(s) 2205
AcsI RAATTY 9 cut(s) 453, 729, 895, 1141, 1285, 1504, 1701, 2005, 2242
AcuI CTGAAG 4 cut(s) 470, 1794, 2064, 2874
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 4 cut(s) 937, 1736, 1773, 2120
AflII CTTAAG 3 cut(s) 268, 1021, 2492
AflIII ACRYGT 4 cut(s) 632, 1528, 2172, 2742
AhlI ACTAGT 1 cut(s) 2797
AjuI GAANNNNNNNTTGG 4 cut(s) 194, 226, 1712, 1744
Alw21I GWGCWC 1 cut(s) 2890
Alw26I GTCTC 8 cut(s) 37, 53, 554, 558, 985, 1436, 1905, 1936
AlwI GGATC 5 cut(s) 101, 228, 241, 520, 2629
AlwNI CAGNNNCTG 1 cut(s) 1817
Ama87I CYCGRG 1 cut(s) 557
Aor13HI TCCGGA 1 cut(s) 1388
AoxI GGCC 2 cut(s) 813, 2205
ApeKI GCWGC 3 cut(s) 1213, 2189, 2636
ApoI RAATTY 9 cut(s) 453, 729, 895, 1141, 1285, 1504, 1701, 2005, 2242
ArsI GACNNNNNNTTYG 4 cut(s) 836, 868, 959, 991
AseI ATTAAT 1 cut(s) 579
Asp700I GAANNNNTTC 2 cut(s) 141, 1323
AspA2I CCTAGG 2 cut(s) 1351, 1597
AspLEI GCGC 1 cut(s) 3009
AspS9I GGNCC 2 cut(s) 813, 1625
AsuC2I CCSGG 3 cut(s) 116, 1936, 2183
AsuHPI GGTGA 5 cut(s) 160, 208, 977, 2147, 2531
AsuNHI GCTAGC 1 cut(s) 79
AvaI CYCGRG 1 cut(s) 557
AvaII GGWCC 1 cut(s) 1625
AvrII CCTAGG 2 cut(s) 1351, 1597
BamHI GGATCC 1 cut(s) 233
BanI GGYRCC 3 cut(s) 377, 889, 2837
BanII GRGCYC 1 cut(s) 218
BarI GAAGNNNNNNTAC 2 cut(s) 658, 690
BbsI GAAGAC 2 cut(s) 734, 2786
Bbv12I GWGCWC 1 cut(s) 2890
BbvI GCAGC 3 cut(s) 1225, 2201, 2623
BceAI ACGGC 1 cut(s) 178
BciVI GTATCC 1 cut(s) 2677
BclI TGATCA 2 cut(s) 990, 2737
BcnI CCSGG 3 cut(s) 116, 1936, 2183
BcoDI GTCTC 8 cut(s) 37, 53, 554, 558, 985, 1436, 1905, 1936
BcuI ACTAGT 1 cut(s) 2797
BfmI CTRYAG 4 cut(s) 172, 570, 1154, 2686
BfrI CTTAAG 3 cut(s) 268, 1021, 2492
BfuAI ACCTGC 3 cut(s) 179, 334, 1161
BfuI GTATCC 1 cut(s) 2677
BglII AGATCT 2 cut(s) 280, 2253
BisI GCNGC 4 cut(s) 1214, 2190, 2287, 2637
BlnI CCTAGG 2 cut(s) 1351, 1597
BlsI GCNGC 4 cut(s) 1215, 2191, 2288, 2638
Bme1390I CCNGG 3 cut(s) 116, 1936, 2183
Bme18I GGWCC 1 cut(s) 1625
BmeT110I CYCGRG 1 cut(s) 557
BmgT120I GGNCC 2 cut(s) 813, 1625
BmiI GGNNCC 9 cut(s) 235, 379, 545, 814, 891, 1483, 1626, 1627, 2839
BmrFI CCNGG 3 cut(s) 116, 1936, 2183
BmrI ACTGGG 3 cut(s) 819, 1557, 2538
BmsI GCATC 5 cut(s) 1131, 1203, 1347, 2359, 2993
BmtI GCTAGC 1 cut(s) 83
BmuI ACTGGG 3 cut(s) 819, 1557, 2538
BpiI GAAGAC 2 cut(s) 734, 2786
BpuEI CTTGAG 4 cut(s) 269, 775, 991, 1736
BpuMI CCSGG 3 cut(s) 116, 1936, 2183
BsaAI YACGTR 2 cut(s) 635, 1520
BsaBI GATNNNNATC 1 cut(s) 238
BsaHI GRCGYC 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 1436
BsaJI CCNNGG 8 cut(s) 427, 834, 916, 1351, 1491, 1570, 1597, 2182
BsaWI WCCGGW 1 cut(s) 1388
Bse1I ACTGG 9 cut(s) 379, 616, 814, 1135, 1563, 1756, 1783, 1959, 2533
Bse3DI GCAATG 4 cut(s) 74, 766, 2296, 2418
Bse8I GATNNNNATC 1 cut(s) 238
BseAI TCCGGA 1 cut(s) 1388
BseDI CCNNGG 8 cut(s) 427, 834, 916, 1351, 1491, 1570, 1597, 2182
BseJI GATNNNNATC 1 cut(s) 238
BseMI GCAATG 4 cut(s) 74, 766, 2296, 2418
BseMII CTCAG 3 cut(s) 996, 1177, 2071
BseNI ACTGG 9 cut(s) 379, 616, 814, 1135, 1563, 1756, 1783, 1959, 2533
BseRI GAGGAG 3 cut(s) 306, 2045, 2915
BseXI GCAGC 3 cut(s) 1225, 2201, 2623
BsgI GTGCAG 1 cut(s) 2622
Bsh1285I CGRYCG 1 cut(s) 2478
BshFI GGCC 2 cut(s) 815, 2207
BshNI GGYRCC 3 cut(s) 377, 889, 2837
BsiEI CGRYCG 1 cut(s) 2478
BsiHKAI GWGCWC 1 cut(s) 2890
BsiHKCI CYCGRG 1 cut(s) 557
BsiSI CCGG 4 cut(s) 115, 1389, 1935, 2182
BslFI GGGAC 2 cut(s) 1638, 1965
BsmAI GTCTC 8 cut(s) 37, 53, 554, 558, 985, 1436, 1905, 1936
BsmBI CGTCTC 2 cut(s) 53, 558
BsmFI GGGAC 2 cut(s) 1638, 1965
BsmI GAATGC 3 cut(s) 1266, 1801, 2697
BsnI GGCC 2 cut(s) 815, 2207
Bso31I GGTCTC 1 cut(s) 1436
BsoBI CYCGRG 1 cut(s) 557
Bsp1286I GDGCHC 2 cut(s) 218, 2890
Bsp13I TCCGGA 1 cut(s) 1388
Bsp1407I TGTACA 1 cut(s) 2118
Bsp19I CCATGG 1 cut(s) 1491
BspACI CCGC 7 cut(s) 187, 954, 1231, 1415, 1782, 1836, 2286
BspANI GGCC 2 cut(s) 815, 2207
BspCNI CTCAG 3 cut(s) 995, 1176, 2070
BspEI TCCGGA 1 cut(s) 1388
BspLI GGNNCC 9 cut(s) 235, 379, 545, 814, 891, 1483, 1626, 1627, 2839
BspMAI CTGCAG 2 cut(s) 176, 1158
BspMI ACCTGC 3 cut(s) 179, 334, 1161
BspOI GCTAGC 1 cut(s) 83
BspPI GGATC 5 cut(s) 101, 228, 241, 520, 2629
BspT107I GGYRCC 3 cut(s) 377, 889, 2837
BspTI CTTAAG 3 cut(s) 268, 1021, 2492
BspTNI GGTCTC 1 cut(s) 1436
BsrDI GCAATG 4 cut(s) 74, 766, 2296, 2418
BsrGI TGTACA 1 cut(s) 2118
BsrI ACTGG 9 cut(s) 379, 616, 814, 1135, 1563, 1756, 1783, 1959, 2533
BssECI CCNNGG 8 cut(s) 427, 834, 916, 1351, 1491, 1570, 1597, 2182
BssNI GRCGYC 1 cut(s) 165
BssT1I CCWWGG 6 cut(s) 427, 834, 916, 1351, 1491, 1597
Bst4CI ACNGT 1 cut(s) 1296
BstACI GRCGYC 1 cut(s) 165
BstAFI CTTAAG 3 cut(s) 268, 1021, 2492
BstAPI GCANNNNNTGC 2 cut(s) 340, 349
BstAUI TGTACA 1 cut(s) 2118
BstBAI YACGTR 2 cut(s) 635, 1520
BstC8I GCNNGC 4 cut(s) 72, 81, 1604, 1871
BstDEI CTNAG 9 cut(s) 260, 467, 586, 982, 1163, 1588, 1652, 2057, 2193
BstDSI CCRYGG 1 cut(s) 1491
BstEII GGTNACC 1 cut(s) 2519
BstHHI GCGC 1 cut(s) 3009
BstMAI GTCTC 8 cut(s) 37, 53, 554, 558, 985, 1436, 1905, 1936
BstMCI CGRYCG 1 cut(s) 2478
BstMWI GCNNNNNNNGC 8 cut(s) 76, 80, 89, 171, 340, 349, 1222, 1823
BstNSI RCATGY 5 cut(s) 2176, 2268, 2537, 2746, 2940
BstPI GGTNACC 1 cut(s) 2519
BstSCI CCNGG 3 cut(s) 114, 1934, 2181
BstSFI CTRYAG 4 cut(s) 172, 570, 1154, 2686
BstV1I GCAGC 3 cut(s) 1225, 2201, 2623
BstV2I GAAGAC 2 cut(s) 734, 2786
BstX2I RGATCY 5 cut(s) 233, 280, 525, 2253, 2621
BstXI CCANNNNNNTGG 3 cut(s) 1115, 1427, 1478
BstYI RGATCY 5 cut(s) 233, 280, 525, 2253, 2621
BsuI GTATCC 1 cut(s) 2677
BsuRI GGCC 2 cut(s) 815, 2207
BtgI CCRYGG 1 cut(s) 1491
BtsIMutI CAGTG 5 cut(s) 623, 2526, 2733, 2850, 2980
BveI ACCTGC 3 cut(s) 179, 334, 1161
Cac8I GCNNGC 4 cut(s) 72, 81, 1604, 1871
CaiI CAGNNNCTG 1 cut(s) 1817
CfoI GCGC 1 cut(s) 3009
Cfr13I GGNCC 2 cut(s) 813, 1625
CseI GACGC 1 cut(s) 154
Csp6I GTAC 4 cut(s) 936, 1735, 1772, 2119
CspCI CAANNNNNGTGG 2 cut(s) 1189, 1224
CviQI GTAC 4 cut(s) 936, 1735, 1772, 2119
DdeI CTNAG 9 cut(s) 260, 467, 586, 982, 1163, 1588, 1652, 2057, 2193
EaeI YGGCCR 1 cut(s) 2205
Eco130I CCWWGG 6 cut(s) 427, 834, 916, 1351, 1491, 1597
Eco24I GRGCYC 1 cut(s) 218
Eco31I GGTCTC 1 cut(s) 1436
Eco32I GATATC 1 cut(s) 2971
Eco47I GGWCC 1 cut(s) 1625
Eco57I CTGAAG 4 cut(s) 470, 1794, 2064, 2874
Eco88I CYCGRG 1 cut(s) 557
Eco91I GGTNACC 1 cut(s) 2519
EcoO109I RGGNCCY 1 cut(s) 1625
EcoO65I GGTNACC 1 cut(s) 2519
EcoRV GATATC 1 cut(s) 2971
EcoT14I CCWWGG 6 cut(s) 427, 834, 916, 1351, 1491, 1597
EcoT38I GRGCYC 1 cut(s) 218
ErhI CCWWGG 6 cut(s) 427, 834, 916, 1351, 1491, 1597
Esp3I CGTCTC 2 cut(s) 53, 558
FaqI GGGAC 2 cut(s) 1638, 1965
FauNDI CATATG 1 cut(s) 2044
FbaI TGATCA 2 cut(s) 990, 2737
FblI GTMKAC 2 cut(s) 178, 2685
Fnu4HI GCNGC 4 cut(s) 1214, 2190, 2287, 2637
FriOI GRGCYC 1 cut(s) 218
Fsp4HI GCNGC 4 cut(s) 1214, 2190, 2287, 2637
GlaI GCGC 1 cut(s) 3008
GluI GCNGC 4 cut(s) 1214, 2190, 2287, 2637
HaeIII GGCC 2 cut(s) 815, 2207
HapII CCGG 4 cut(s) 115, 1389, 1935, 2182
HgaI GACGC 1 cut(s) 154
HhaI GCGC 1 cut(s) 3009
Hin1I GRCGYC 1 cut(s) 165
Hin6I GCGC 1 cut(s) 3007
HinP1I GCGC 1 cut(s) 3007
HincII GTYRAC 4 cut(s) 179, 508, 1150, 1299
HindII GTYRAC 4 cut(s) 179, 508, 1150, 1299
HindIII AAGCTT 3 cut(s) 262, 482, 1871
HpaI GTTAAC 1 cut(s) 1299
HpaII CCGG 4 cut(s) 115, 1389, 1935, 2182
HphI GGTGA 5 cut(s) 160, 208, 977, 2147, 2531
Hpy99I CGWCG 2 cut(s) 183, 716
HpyCH4III ACNGT 1 cut(s) 1296
HpyCH4IV ACGT 5 cut(s) 634, 714, 1046, 1519, 1530
HpyF10VI GCNNNNNNNGC 8 cut(s) 76, 80, 89, 171, 340, 349, 1222, 1823
HpyF3I CTNAG 9 cut(s) 260, 467, 586, 982, 1163, 1588, 1652, 2057, 2193
HpySE526I ACGT 5 cut(s) 634, 714, 1046, 1519, 1530
Hsp92I GRCGYC 1 cut(s) 165
HspAI GCGC 1 cut(s) 3007
KflI GGGWCCC 1 cut(s) 1625
Kpn2I TCCGGA 1 cut(s) 1388
Ksp22I TGATCA 2 cut(s) 990, 2737
KspAI GTTAAC 1 cut(s) 1299
LmnI GCTCC 5 cut(s) 26, 1263, 1766, 2186, 2331
Lsp1109I GCAGC 3 cut(s) 1225, 2201, 2623
LweI GCATC 5 cut(s) 1131, 1203, 1347, 2359, 2993
MaeII ACGT 5 cut(s) 634, 714, 1046, 1519, 1530
MfeI CAATTG 5 cut(s) 29, 400, 1095, 1310, 1472
MflI RGATCY 5 cut(s) 233, 280, 525, 2253, 2621
MhlI GDGCHC 2 cut(s) 218, 2890
MlyI GAGTC 4 cut(s) 39, 1824, 1889, 2905
MroI TCCGGA 1 cut(s) 1388
MroXI GAANNNNTTC 2 cut(s) 141, 1323
MslI CAYNNNNRTG 2 cut(s) 1113, 2772
MspCI CTTAAG 3 cut(s) 268, 1021, 2492
MspI CCGG 4 cut(s) 115, 1389, 1935, 2182
MspR9I CCNGG 3 cut(s) 116, 1936, 2183
MunI CAATTG 5 cut(s) 29, 400, 1095, 1310, 1472
Mva1269I GAATGC 3 cut(s) 1266, 1801, 2697
MwoI GCNNNNNNNGC 8 cut(s) 76, 80, 89, 171, 340, 349, 1222, 1823
NciI CCSGG 3 cut(s) 116, 1936, 2183
NcoI CCATGG 1 cut(s) 1491
NdeI CATATG 1 cut(s) 2044
NheI GCTAGC 1 cut(s) 79
NlaIV GGNNCC 9 cut(s) 235, 379, 545, 814, 891, 1483, 1626, 1627, 2839
NmeAIII GCCGAG 1 cut(s) 1551
NmuCI GTSAC 8 cut(s) 118, 166, 196, 965, 1158, 2519, 2750, 2848
NspI RCATGY 5 cut(s) 2176, 2268, 2537, 2746, 2940
PaeR7I CTCGAG 1 cut(s) 557
PaqCI CACCTGC 1 cut(s) 179
PciI ACATGT 2 cut(s) 2172, 2742
PctI GAATGC 3 cut(s) 1266, 1801, 2697
PdmI GAANNNNTTC 2 cut(s) 141, 1323
PfeI GAWTC 6 cut(s) 1513, 1660, 1720, 2761, 2816, 2948
PflMI CCANNNNNTGG 2 cut(s) 2360, 2648
PkrI GCNGC 4 cut(s) 1215, 2191, 2288, 2638
Ple19I CGATCG 1 cut(s) 2478
PleI GAGTC 4 cut(s) 39, 1824, 1888, 2905
PpsI GAGTC 4 cut(s) 39, 1824, 1888, 2905
Ppu21I YACGTR 2 cut(s) 635, 1520
PpuMI RGGWCCY 1 cut(s) 1625
PscI ACATGT 2 cut(s) 2172, 2742
PshBI ATTAAT 1 cut(s) 579
Psp5II RGGWCCY 1 cut(s) 1625
PspEI GGTNACC 1 cut(s) 2519
PspN4I GGNNCC 9 cut(s) 235, 379, 545, 814, 891, 1483, 1626, 1627, 2839
PspPI GGNCC 2 cut(s) 813, 1625
PspPPI RGGWCCY 1 cut(s) 1625
PsrI GAACNNNNNNTAC 2 cut(s) 1718, 1750
PstI CTGCAG 2 cut(s) 176, 1158
PstNI CAGNNNCTG 1 cut(s) 1817
PsuI RGATCY 5 cut(s) 233, 280, 525, 2253, 2621
PvuI CGATCG 1 cut(s) 2478
RsaI GTAC 4 cut(s) 937, 1736, 1773, 2120
RsaNI GTAC 4 cut(s) 936, 1735, 1772, 2119
RseI CAYNNNNRTG 2 cut(s) 1113, 2772
SalI GTCGAC 1 cut(s) 177
SatI GCNGC 4 cut(s) 1214, 2190, 2287, 2637
Sau96I GGNCC 2 cut(s) 813, 1625
SchI GAGTC 4 cut(s) 39, 1824, 1889, 2905
ScrFI CCNGG 3 cut(s) 116, 1936, 2183
SduI GDGCHC 2 cut(s) 218, 2890
SfaNI GCATC 5 cut(s) 1131, 1203, 1347, 2359, 2993
SfcI CTRYAG 4 cut(s) 172, 570, 1154, 2686
Sfr274I CTCGAG 1 cut(s) 557
SinI GGWCC 1 cut(s) 1625
SlaI CTCGAG 1 cut(s) 557
SmiMI CAYNNNNRTG 2 cut(s) 1113, 2772
SmlI CTYRAG 8 cut(s) 268, 284, 557, 754, 970, 1021, 1715, 2492
SmoI CTYRAG 8 cut(s) 268, 284, 557, 754, 970, 1021, 1715, 2492
SpeI ACTAGT 1 cut(s) 2797
SsiI CCGC 7 cut(s) 187, 954, 1231, 1415, 1782, 1836, 2286
SspI AATATT 1 cut(s) 1324
StyD4I CCNGG 3 cut(s) 114, 1934, 2181
StyI CCWWGG 6 cut(s) 427, 834, 916, 1351, 1491, 1597
TaaI ACNGT 1 cut(s) 1296
TaiI ACGT 5 cut(s) 637, 717, 1049, 1522, 1533
TaqI TCGA 8 cut(s) 48, 178, 558, 711, 795, 1663, 1855, 2239
TatI WGTACW 1 cut(s) 2118
TauI GCSGC 1 cut(s) 2289
TfiI GAWTC 6 cut(s) 1513, 1660, 1720, 2761, 2816, 2948
TscAI CASTG 5 cut(s) 623, 2533, 2740, 2857, 2980
TseFI GTSAC 8 cut(s) 118, 166, 196, 965, 1158, 2519, 2750, 2848
TseI GCWGC 3 cut(s) 1213, 2189, 2636
Tsp45I GTSAC 8 cut(s) 118, 166, 196, 965, 1158, 2519, 2750, 2848
TspGWI ACGGA 2 cut(s) 77, 778
TspRI CASTG 5 cut(s) 623, 2533, 2740, 2857, 2980
Van91I CCANNNNNTGG 2 cut(s) 2360, 2648
Vha464I CTTAAG 3 cut(s) 268, 1021, 2492
VpaK11BI GGWCC 1 cut(s) 1625
VspI ATTAAT 1 cut(s) 579
XapI RAATTY 9 cut(s) 453, 729, 895, 1141, 1285, 1504, 1701, 2005, 2242
XbaI TCTAGA 1 cut(s) 1327
XceI RCATGY 5 cut(s) 2176, 2268, 2537, 2746, 2940
XhoI CTCGAG 1 cut(s) 557
XmaJI CCTAGG 2 cut(s) 1351, 1597
XmiI GTMKAC 2 cut(s) 178, 2685
XmnI GAANNNNTTC 2 cut(s) 141, 1323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.