FvH4_7g17560

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0012890
Physical Location & Seq
Reverse (-)
14886646 .. 14887572
927 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g17560.t1

Sequence Viewer

Length: 600 bp
ATGGGCAACTGTGTTTTCAAAGGGTTTGGAGCTGAGGCAGATGATCAAACAGAGATTGTGAAGGTGGTGACCTCTGGTGGCATCATGGAGATCTATGCACCGATCGCCGCCAGCACCATAACCGACGAGTTTCCCGGCCAAGCCATCTTCCGCAGCCACGACCTCTCGGGTCAGCCTCTCCTACACAACGAAAAACTTCACCCCCATGAGGTCTACCATCTCCTACCTCTAAACCCCTACAGAACACCCATCAAAACACCAAGCTTATATCATCAGAACAGCGGCATATGCACCCCATATCGCATATCATCATGCGATAGTTACCGGAGAAAGAACGCTGATCCGGAACCATTTACGAGATATAGTAAAAAGAGCAGCGGCGAGTGGAAGGTGAAGCTGGTCATAAGCCCTGAGGAGCTATCGGAGATATTGTCGCAGGGGGATAAAACTAAGGAGTTGATAGATAGCGCGAGGACGGTGGCCAAGTGTGGAAGTGCCGGAGGCTCGTCGGTAGCTGGATCCGAACATTCGAGTACTGTGTCTAGTATTAGGTGGAAGGAAGCTAGCATGATCAATAGTATGCTCAGATACAATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

200

Amino Acids

21.92

Weight (kDa)

7.05

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 162 1.9e-16 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61920
fragaria_vesca FvH4_7g17560
malus_domestica MD01G1088400.v1.1 MD07G1158800.v1.1 MD08G1186200.v1.1
prunus_persica Prupe.2G196900_v2.0.a1
pyrus_communis pycom01g11210 pycom07g15530
rosa_chinensis RchiOBHm_Chr1g0361011
rosa_laevigata RLG00000027757
rosa_multiflora Rmu_sc0000677.1_g000009
rosa_roxburghii Rroxscaffold_4G00295530
rosa_rugosa Rorug01G0284200
rosa_samantha Rh1AG295800 Rh1BG260500 Rh1CG277700 Rh1DG290500
rosa_wichuraiana Rw1G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 213
AccII CGCG 1 cut(s) 470
AccIII TCCGGA 1 cut(s) 343
AciI CCGC 4 cut(s) 108, 151, 282, 378
AclWI GGATC 3 cut(s) 335, 513, 526
AcoI YGGCCR 2 cut(s) 136, 480
AfaI GTAC 1 cut(s) 535
AfiI CCNNNNNNNGG 1 cut(s) 208
AgsI TTSAA 1 cut(s) 19
AluBI AGCT 6 cut(s) 32, 264, 397, 418, 515, 563
AluI AGCT 6 cut(s) 32, 264, 397, 418, 515, 563
AlwI GGATC 3 cut(s) 335, 513, 526
Ama87I CYCGRG 1 cut(s) 166
Aor13HI TCCGGA 1 cut(s) 343
AoxI GGCC 2 cut(s) 136, 480
ApeKI GCWGC 2 cut(s) 153, 375
Asp700I GAANNNNTTC 1 cut(s) 195
AspLEI GCGC 1 cut(s) 470
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 3 cut(s) 79, 191, 403
AsuNHI GCTAGC 1 cut(s) 563
AvaI CYCGRG 1 cut(s) 166
AxyI CCTNAGG 1 cut(s) 411
BalI TGGCCA 1 cut(s) 482
BamHI GGATCC 1 cut(s) 518
BbvCI CCTCAGC 1 cut(s) 33
BbvI GCAGC 2 cut(s) 165, 387
BccI CCATC 3 cut(s) 152, 225, 257
BclI TGATCA 2 cut(s) 43, 570
BcnI CCSGG 1 cut(s) 135
BfaI CTAG 2 cut(s) 543, 564
BfmI CTRYAG 1 cut(s) 238
BglII AGATCT 1 cut(s) 90
BisI GCNGC 5 cut(s) 108, 154, 283, 376, 379
BlsI GCNGC 5 cut(s) 109, 155, 284, 377, 380
BmcAI AGTACT 1 cut(s) 535
Bme1390I CCNGG 1 cut(s) 135
BmeT110I CYCGRG 1 cut(s) 166
BmiI GGNNCC 2 cut(s) 348, 520
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 90
BmtI GCTAGC 1 cut(s) 567
Bpu10I CCTNAGC 1 cut(s) 33
BpuMI CCSGG 1 cut(s) 135
BsaWI WCCGGW 2 cut(s) 324, 343
BsaXI ACNNNNNCTCC 2 cut(s) 416, 446
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse21I CCTNAGG 1 cut(s) 411
BseAI TCCGGA 1 cut(s) 343
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMII CTCAG 3 cut(s) 24, 402, 598
BseRI GAGGAG 1 cut(s) 428
BseXI GCAGC 2 cut(s) 165, 387
Bsh1236I CGCG 1 cut(s) 470
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 2 cut(s) 138, 482
BsiEI CGRYCG 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 166
BsiSI CCGG 4 cut(s) 135, 325, 344, 498
BslI CCNNNNNNNGG 1 cut(s) 208
BsnI GGCC 2 cut(s) 138, 482
BsoBI CYCGRG 1 cut(s) 166
Bsp13I TCCGGA 1 cut(s) 343
Bsp143I GATC 6 cut(s) 43, 90, 102, 340, 518, 570
BspACI CCGC 4 cut(s) 108, 151, 282, 378
BspANI GGCC 2 cut(s) 138, 482
BspCNI CTCAG 3 cut(s) 25, 403, 597
BspEI TCCGGA 1 cut(s) 343
BspFNI CGCG 1 cut(s) 470
BspLI GGNNCC 2 cut(s) 348, 520
BspOI GCTAGC 1 cut(s) 567
BspPI GGATC 3 cut(s) 335, 513, 526
BssMI GATC 6 cut(s) 43, 90, 102, 340, 518, 570
Bst4CI ACNGT 3 cut(s) 11, 478, 538
BstC8I GCNNGC 2 cut(s) 112, 565
BstDEI CTNAG 4 cut(s) 33, 411, 450, 584
BstEII GGTNACC 1 cut(s) 67
BstFNI CGCG 1 cut(s) 470
BstHHI GCGC 1 cut(s) 470
BstKTI GATC 6 cut(s) 46, 93, 105, 343, 521, 573
BstMBI GATC 6 cut(s) 43, 90, 102, 340, 518, 570
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 2 cut(s) 104, 288
BstPI GGTNACC 1 cut(s) 67
BstSCI CCNGG 1 cut(s) 133
BstSFI CTRYAG 1 cut(s) 238
BstUI CGCG 1 cut(s) 470
BstV1I GCAGC 2 cut(s) 165, 387
BstX2I RGATCY 2 cut(s) 90, 518
BstYI RGATCY 2 cut(s) 90, 518
Bsu36I CCTNAGG 1 cut(s) 411
BsuRI GGCC 2 cut(s) 138, 482
Cac8I GCNNGC 2 cut(s) 112, 565
CfoI GCGC 1 cut(s) 470
Csp6I GTAC 1 cut(s) 534
CviAII CATG 4 cut(s) 85, 206, 312, 568
CviQI GTAC 1 cut(s) 534
DdeI CTNAG 4 cut(s) 33, 411, 450, 584
DpnI GATC 6 cut(s) 45, 92, 104, 342, 520, 572
DpnII GATC 6 cut(s) 43, 90, 102, 340, 518, 570
EaeI YGGCCR 2 cut(s) 136, 480
Eco81I CCTNAGG 1 cut(s) 411
Eco88I CYCGRG 1 cut(s) 166
Eco91I GGTNACC 1 cut(s) 67
EcoO65I GGTNACC 1 cut(s) 67
FaeI CATG 4 cut(s) 88, 209, 315, 571
FatI CATG 4 cut(s) 84, 205, 311, 567
FauNDI CATATG 1 cut(s) 287
FbaI TGATCA 2 cut(s) 43, 570
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 5 cut(s) 108, 154, 283, 376, 379
Fsp4HI GCNGC 5 cut(s) 108, 154, 283, 376, 379
FspBI CTAG 2 cut(s) 543, 564
GlaI GCGC 1 cut(s) 469
GluI GCNGC 5 cut(s) 108, 154, 283, 376, 379
HaeIII GGCC 2 cut(s) 138, 482
HapII CCGG 4 cut(s) 135, 325, 344, 498
HhaI GCGC 1 cut(s) 470
Hin1II CATG 4 cut(s) 88, 209, 315, 571
Hin6I GCGC 1 cut(s) 468
HinP1I GCGC 1 cut(s) 468
HindIII AAGCTT 1 cut(s) 262
HpaII CCGG 4 cut(s) 135, 325, 344, 498
HphI GGTGA 3 cut(s) 79, 191, 403
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 4 cut(s) 276, 424, 523, 587
Hpy188III TCNNGA 1 cut(s) 344
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 2 cut(s) 128, 511
HpyAV CCTTC 3 cut(s) 55, 382, 550
HpyCH4III ACNGT 3 cut(s) 11, 478, 538
HpyCH4V TGCA 2 cut(s) 98, 291
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 288
HpyF3I CTNAG 4 cut(s) 33, 411, 450, 584
Hsp92II CATG 4 cut(s) 88, 209, 315, 571
HspAI GCGC 1 cut(s) 468
Kpn2I TCCGGA 1 cut(s) 343
Ksp22I TGATCA 2 cut(s) 43, 570
Kzo9I GATC 6 cut(s) 43, 90, 102, 340, 518, 570
LmnI GCTCC 2 cut(s) 29, 415
Lsp1109I GCAGC 2 cut(s) 165, 387
LweI GCATC 1 cut(s) 90
MaeI CTAG 2 cut(s) 543, 564
MaeIII GTNAC 2 cut(s) 67, 320
MalI GATC 6 cut(s) 45, 92, 104, 342, 520, 572
MboI GATC 6 cut(s) 43, 90, 102, 340, 518, 570
MboII GAAGA 1 cut(s) 139
MflI RGATCY 2 cut(s) 90, 518
MlsI TGGCCA 1 cut(s) 482
MluNI TGGCCA 1 cut(s) 482
MnlI CCTC 9 cut(s) 28, 82, 173, 186, 202, 237, 406, 465, 494
Mox20I TGGCCA 1 cut(s) 482
MroI TCCGGA 1 cut(s) 343
MroXI GAANNNNTTC 1 cut(s) 195
MscI TGGCCA 1 cut(s) 482
MslI CAYNNNNRTG 1 cut(s) 204
Msp20I TGGCCA 1 cut(s) 482
MspA1I CMGCKG 2 cut(s) 282, 378
MspI CCGG 4 cut(s) 135, 325, 344, 498
MspR9I CCNGG 1 cut(s) 135
MvnI CGCG 1 cut(s) 470
MwoI GCNNNNNNNGC 2 cut(s) 104, 288
NciI CCSGG 1 cut(s) 135
NdeI CATATG 1 cut(s) 287
NdeII GATC 6 cut(s) 43, 90, 102, 340, 518, 570
NheI GCTAGC 1 cut(s) 563
NlaIII CATG 4 cut(s) 88, 209, 315, 571
NlaIV GGNNCC 2 cut(s) 348, 520
NmuCI GTSAC 1 cut(s) 67
PdmI GAANNNNTTC 1 cut(s) 195
PkrI GCNGC 5 cut(s) 109, 155, 284, 377, 380
Ple19I CGATCG 1 cut(s) 105
PspEI GGTNACC 1 cut(s) 67
PspN4I GGNNCC 2 cut(s) 348, 520
PsuI RGATCY 2 cut(s) 90, 518
PvuI CGATCG 1 cut(s) 105
RsaI GTAC 1 cut(s) 535
RsaNI GTAC 1 cut(s) 534
RseI CAYNNNNRTG 1 cut(s) 204
SatI GCNGC 5 cut(s) 108, 154, 283, 376, 379
Sau3AI GATC 6 cut(s) 43, 90, 102, 340, 518, 570
ScaI AGTACT 1 cut(s) 535
ScrFI CCNGG 1 cut(s) 135
SfaNI GCATC 1 cut(s) 90
SfcI CTRYAG 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 204
SsiI CCGC 4 cut(s) 108, 151, 282, 378
SspMI CTAG 2 cut(s) 543, 564
StyD4I CCNGG 1 cut(s) 133
TaaI ACNGT 3 cut(s) 11, 478, 538
TaqI TCGA 1 cut(s) 530
TatI WGTACW 1 cut(s) 533
TauI GCSGC 3 cut(s) 110, 285, 381
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 2 cut(s) 153, 375
Tsp45I GTSAC 1 cut(s) 67
XmiI GTMKAC 1 cut(s) 213
XmnI GAANNNNTTC 1 cut(s) 195
XspI CTAG 2 cut(s) 543, 564
ZrmI AGTACT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.