Rmu_sc0000677.1_g000009

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000677.1
OG0012890
Physical Location & Seq
Forward (+)
48827 .. 49396
570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000677.1_g000009.1.cds

Sequence Viewer

Length: 570 bp
atgggcaattgtgtgtttaaagggttcggagctgagacagatgatcaagcagagatggtcaaggtgatgacctccagtggcggcatcatggagctctatgcaccgatcaccgccagcacaataaccgacgagttccccggccacgccatcttccgaagcctcgacctcttggcccagcctctcctacacaacgaagaacttcacccccacgagctctaccatctgctacctctaaatccctacaaaacacccatcaaaacaccaaacttatatcatcagaacagcggcctatgcacgccgtatcgcatgtcatcatgcgacagttaccagagaaagaaggctgatccggaagcgtttccggagtacggtaacaagagcagcggcgtgtggaaggtaaacctggtcataagccctgaggagctatcagagatattgtcgcagggagctagaactgaggagttgattgatagcgcgaggacggtggccaagtgtggaagtaccggaggctcgtcggtagctggttccgaacagtggagtactgtttctagtaggtggaaagaagcgccatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

189

Amino Acids

20.75

Weight (kDa)

5.35

Isoelectric Point (pI)

51.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61920
fragaria_vesca FvH4_7g17560
malus_domestica MD01G1088400.v1.1 MD07G1158800.v1.1 MD08G1186200.v1.1
prunus_persica Prupe.2G196900_v2.0.a1
pyrus_communis pycom01g11210 pycom07g15530
rosa_chinensis RchiOBHm_Chr1g0361011
rosa_laevigata RLG00000027757
rosa_multiflora Rmu_sc0000677.1_g000009
rosa_roxburghii Rroxscaffold_4G00295530
rosa_rugosa Rorug01G0284200
rosa_samantha Rh1AG295800 Rh1BG260500 Rh1CG277700 Rh1DG290500
rosa_wichuraiana Rw1G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 473
AccIII TCCGGA 2 cut(s) 346, 358
AciI CCGC 4 cut(s) 81, 111, 285, 381
AclWI GGATC 1 cut(s) 338
AcoI YGGCCR 2 cut(s) 139, 483
AfaI GTAC 3 cut(s) 365, 499, 538
AfiI CCNNNNNNNGG 2 cut(s) 365, 531
AjnI CCWGG 1 cut(s) 399
AluBI AGCT 6 cut(s) 32, 94, 214, 421, 446, 518
AluI AGCT 6 cut(s) 32, 94, 214, 421, 446, 518
Alw21I GWGCWC 2 cut(s) 96, 216
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 1 cut(s) 338
Aor13HI TCCGGA 2 cut(s) 346, 358
AoxI GGCC 4 cut(s) 139, 171, 286, 483
ApeKI GCWGC 1 cut(s) 378
Asp700I GAANNNNTTC 2 cut(s) 198, 354
AspLEI GCGC 2 cut(s) 473, 565
AspS9I GGNCC 1 cut(s) 172
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 3 cut(s) 76, 100, 194
AxyI CCTNAGG 1 cut(s) 414
BalI TGGCCA 1 cut(s) 485
BanII GRGCYC 2 cut(s) 96, 216
BauI CACGAG 1 cut(s) 209
Bbv12I GWGCWC 2 cut(s) 96, 216
BbvI GCAGC 1 cut(s) 390
BccI CCATC 4 cut(s) 49, 155, 228, 260
BceAI ACGGC 1 cut(s) 283
BciT130I CCWGG 1 cut(s) 401
BclI TGATCA 1 cut(s) 43
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 29
BfaI CTAG 2 cut(s) 447, 546
BfoI RGCGCY 1 cut(s) 566
BisI GCNGC 4 cut(s) 82, 286, 379, 382
BlsI GCNGC 4 cut(s) 83, 287, 380, 383
BmcAI AGTACT 1 cut(s) 538
Bme1390I CCNGG 2 cut(s) 138, 401
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 1 cut(s) 523
BmrFI CCNGG 2 cut(s) 138, 401
BmsI GCATC 1 cut(s) 93
BpmI CTGGAG 1 cut(s) 58
BpuMI CCSGG 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 136
BsaWI WCCGGW 3 cut(s) 346, 358, 500
Bsc4I CCNNNNNNNGG 2 cut(s) 365, 531
Bse1I ACTGG 1 cut(s) 75
Bse21I CCTNAGG 1 cut(s) 414
BseAI TCCGGA 2 cut(s) 346, 358
BseBI CCWGG 1 cut(s) 401
BseDI CCNNGG 1 cut(s) 136
BseLI CCNNNNNNNGG 2 cut(s) 365, 531
BseMII CTCAG 3 cut(s) 24, 405, 444
BseNI ACTGG 1 cut(s) 75
BseRI GAGGAG 2 cut(s) 431, 470
BseXI GCAGC 1 cut(s) 390
BseYI CCCAGC 1 cut(s) 174
Bsh1236I CGCG 1 cut(s) 473
BshFI GGCC 4 cut(s) 141, 173, 288, 485
BsiHKAI GWGCWC 2 cut(s) 96, 216
BsiSI CCGG 4 cut(s) 138, 347, 359, 501
BslI CCNNNNNNNGG 2 cut(s) 365, 531
BsmAI GTCTC 1 cut(s) 29
BsnI GGCC 4 cut(s) 141, 173, 288, 485
Bsp1286I GDGCHC 2 cut(s) 96, 216
Bsp13I TCCGGA 2 cut(s) 346, 358
Bsp143I GATC 3 cut(s) 43, 105, 343
BspACI CCGC 4 cut(s) 81, 111, 285, 381
BspANI GGCC 4 cut(s) 141, 173, 288, 485
BspCNI CTCAG 3 cut(s) 25, 406, 445
BspEI TCCGGA 2 cut(s) 346, 358
BspFNI CGCG 1 cut(s) 473
BspLI GGNNCC 1 cut(s) 523
BspPI GGATC 1 cut(s) 338
BsrI ACTGG 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 3 cut(s) 43, 105, 343
BssSI CACGAG 1 cut(s) 209
Bst2BI CACGAG 1 cut(s) 209
Bst2UI CCWGG 1 cut(s) 401
Bst4CI ACNGT 5 cut(s) 323, 368, 481, 531, 541
BstC8I GCNNGC 2 cut(s) 115, 296
BstDEI CTNAG 3 cut(s) 33, 414, 453
BstFNI CGCG 1 cut(s) 473
BstH2I RGCGCY 1 cut(s) 566
BstHHI GCGC 2 cut(s) 473, 565
BstKTI GATC 3 cut(s) 46, 108, 346
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 3 cut(s) 43, 105, 343
BstMWI GCNNNNNNNGC 1 cut(s) 291
BstNI CCWGG 1 cut(s) 401
BstNSI RCATGY 1 cut(s) 310
BstSCI CCNGG 2 cut(s) 136, 399
BstUI CGCG 1 cut(s) 473
BstV1I GCAGC 1 cut(s) 390
Bsu36I CCTNAGG 1 cut(s) 414
BsuRI GGCC 4 cut(s) 141, 173, 288, 485
BtsIMutI CAGTG 2 cut(s) 82, 536
Cac8I GCNNGC 2 cut(s) 115, 296
CfoI GCGC 2 cut(s) 473, 565
Cfr13I GGNCC 1 cut(s) 172
CsiI ACCWGGT 1 cut(s) 399
Csp6I GTAC 3 cut(s) 364, 498, 537
CviAII CATG 4 cut(s) 88, 307, 315, 567
CviQI GTAC 3 cut(s) 364, 498, 537
DdeI CTNAG 3 cut(s) 33, 414, 453
DpnI GATC 3 cut(s) 45, 107, 345
DpnII GATC 3 cut(s) 43, 105, 343
DraI TTTAAA 1 cut(s) 19
EaeI YGGCCR 2 cut(s) 139, 483
Ecl136II GAGCTC 2 cut(s) 94, 214
Eco24I GRGCYC 2 cut(s) 96, 216
Eco53kI GAGCTC 2 cut(s) 94, 214
Eco81I CCTNAGG 1 cut(s) 414
EcoICRI GAGCTC 2 cut(s) 94, 214
EcoRII CCWGG 1 cut(s) 399
EcoT38I GRGCYC 2 cut(s) 96, 216
FaeI CATG 4 cut(s) 91, 310, 318, 570
FaiI YATR 8 cut(s) 89, 99, 271, 292, 308, 316, 407, 568
FatI CATG 4 cut(s) 87, 306, 314, 566
FbaI TGATCA 1 cut(s) 43
Fnu4HI GCNGC 4 cut(s) 82, 286, 379, 382
FriOI GRGCYC 2 cut(s) 96, 216
Fsp4HI GCNGC 4 cut(s) 82, 286, 379, 382
FspBI CTAG 2 cut(s) 447, 546
GlaI GCGC 2 cut(s) 472, 564
GluI GCNGC 4 cut(s) 82, 286, 379, 382
GsaI CCCAGC 1 cut(s) 178
GsuI CTGGAG 1 cut(s) 58
HaeII RGCGCY 1 cut(s) 566
HaeIII GGCC 4 cut(s) 141, 173, 288, 485
HapII CCGG 4 cut(s) 138, 347, 359, 501
HhaI GCGC 2 cut(s) 473, 565
Hin1II CATG 4 cut(s) 91, 310, 318, 570
Hin6I GCGC 2 cut(s) 471, 563
HinP1I GCGC 2 cut(s) 471, 563
HpaII CCGG 4 cut(s) 138, 347, 359, 501
HphI GGTGA 3 cut(s) 76, 100, 194
Hpy166II GTNNAC 1 cut(s) 397
Hpy188I TCNGA 5 cut(s) 29, 155, 279, 427, 526
Hpy188III TCNNGA 2 cut(s) 347, 359
Hpy8I GTNNAC 1 cut(s) 397
Hpy99I CGWCG 2 cut(s) 131, 514
HpyAV CCTTC 2 cut(s) 331, 385
HpyCH4III ACNGT 5 cut(s) 323, 368, 481, 531, 541
HpyCH4V TGCA 2 cut(s) 101, 294
HpyF10VI GCNNNNNNNGC 1 cut(s) 291
HpyF3I CTNAG 3 cut(s) 33, 414, 453
Hsp92II CATG 4 cut(s) 91, 310, 318, 570
HspAI GCGC 2 cut(s) 471, 563
Kpn2I TCCGGA 2 cut(s) 346, 358
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 3 cut(s) 43, 105, 343
LmnI GCTCC 4 cut(s) 29, 91, 418, 443
Lsp1109I GCAGC 1 cut(s) 390
LweI GCATC 1 cut(s) 93
MabI ACCWGGT 1 cut(s) 399
MaeI CTAG 2 cut(s) 447, 546
MaeIII GTNAC 2 cut(s) 323, 368
MalI GATC 3 cut(s) 45, 107, 345
MboI GATC 3 cut(s) 43, 105, 343
MboII GAAGA 2 cut(s) 142, 206
MfeI CAATTG 1 cut(s) 7
MhlI GDGCHC 2 cut(s) 96, 216
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 1 cut(s) 7
MluNI TGGCCA 1 cut(s) 485
MnlI CCTC 9 cut(s) 82, 170, 176, 189, 240, 409, 448, 468, 497
Mox20I TGGCCA 1 cut(s) 485
MroI TCCGGA 2 cut(s) 346, 358
MroXI GAANNNNTTC 2 cut(s) 198, 354
MscI TGGCCA 1 cut(s) 485
MseI TTAA 1 cut(s) 18
Msp20I TGGCCA 1 cut(s) 485
MspA1I CMGCKG 2 cut(s) 285, 381
MspI CCGG 4 cut(s) 138, 347, 359, 501
MspR9I CCNGG 2 cut(s) 138, 401
MunI CAATTG 1 cut(s) 7
MvaI CCWGG 1 cut(s) 401
MvnI CGCG 1 cut(s) 473
MwoI GCNNNNNNNGC 1 cut(s) 291
NciI CCSGG 1 cut(s) 138
NdeII GATC 3 cut(s) 43, 105, 343
NlaIII CATG 4 cut(s) 91, 310, 318, 570
NlaIV GGNNCC 1 cut(s) 523
NspI RCATGY 1 cut(s) 310
PdmI GAANNNNTTC 2 cut(s) 198, 354
PkrI GCNGC 4 cut(s) 83, 287, 380, 383
Psp124BI GAGCTC 2 cut(s) 96, 216
Psp6I CCWGG 1 cut(s) 399
PspFI CCCAGC 1 cut(s) 174
PspGI CCWGG 1 cut(s) 399
PspN4I GGNNCC 1 cut(s) 523
PspPI GGNCC 1 cut(s) 172
RsaI GTAC 3 cut(s) 365, 499, 538
RsaNI GTAC 3 cut(s) 364, 498, 537
SacI GAGCTC 2 cut(s) 96, 216
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 4 cut(s) 82, 286, 379, 382
Sau3AI GATC 3 cut(s) 43, 105, 343
Sau96I GGNCC 1 cut(s) 172
ScaI AGTACT 1 cut(s) 538
ScrFI CCNGG 2 cut(s) 138, 401
SduI GDGCHC 2 cut(s) 96, 216
SexAI ACCWGGT 1 cut(s) 399
SfaNI GCATC 1 cut(s) 93
Sse9I AATT 1 cut(s) 7
SsiI CCGC 4 cut(s) 81, 111, 285, 381
SspMI CTAG 2 cut(s) 447, 546
SstI GAGCTC 2 cut(s) 96, 216
StyD4I CCNGG 2 cut(s) 136, 399
TaaI ACNGT 5 cut(s) 323, 368, 481, 531, 541
TaqI TCGA 1 cut(s) 162
TasI AATT 1 cut(s) 7
TatI WGTACW 1 cut(s) 536
TauI GCSGC 3 cut(s) 84, 288, 384
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 2 cut(s) 82, 536
TseI GCWGC 1 cut(s) 378
TspRI CASTG 2 cut(s) 82, 536
XceI RCATGY 1 cut(s) 310
XmnI GAANNNNTTC 2 cut(s) 198, 354
XspI CTAG 2 cut(s) 447, 546
ZrmI AGTACT 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.