pycom01g11210

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012890
Physical Location & Seq
Reverse (-)
12167203 .. 12167751
549 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g11210.1

Sequence Viewer

Length: 549 bp
ATGGGAAATTGTGTGTTTAAAGGGTTTGAAGAGGCAGAAGAGATGGTGAAAGTGGTGACATCCAGTGGGGGAATCATGGAGCTCTTTGCTCCCATCACAGCTAACTCCATAACCAATGAGTTCCCAGGCCATGCCATCTTCCGCAGCCCTGACCTCTTCGCCCAACCTCTTCTCCACAACGAAGAGCTTCATCCCAGAGAGCCTTACTATCTCCTACCTCTCAACCCCTACAAAACCCCAAAGAATCATGATATTAATGCAAACCCTAACGGCGCCGTTTGTACACCGTATCGCATGACGTGTGACAGCCAAGGCAAGAGAGCGGCAGACCCTGAAGTTTTTCCGAGGTACAATAGTAGTGGGGTTTGGAAGGTGAAGCTGGTGATAAGTCCAGAGCAGCTTTCTGAGATTTTGTCGCAGGAGGCTCGCACTGAGGCTTTGATTGAAAGCGTGAGGACGGTGGCCAAATGCGGCACCGGAGGGAACTCGTCGGTGGCAAGTTCCGATCAGTGGAGTGTGTCTAGTAGTTGGAAGGAGTTGTCATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

183

Amino Acids

20.01

Weight (kDa)

5.4

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 171 7.5e-22 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61920
fragaria_vesca FvH4_7g17560
malus_domestica MD01G1088400.v1.1 MD07G1158800.v1.1 MD08G1186200.v1.1
prunus_persica Prupe.2G196900_v2.0.a1
pyrus_communis pycom01g11210 pycom07g15530
rosa_chinensis RchiOBHm_Chr1g0361011
rosa_laevigata RLG00000027757
rosa_multiflora Rmu_sc0000677.1_g000009
rosa_roxburghii Rroxscaffold_4G00295530
rosa_rugosa Rorug01G0284200
rosa_samantha Rh1AG295800 Rh1BG260500 Rh1CG277700 Rh1DG290500
rosa_wichuraiana Rw1G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 272, 473
AccBSI CCGCTC 1 cut(s) 323
AciI CCGC 3 cut(s) 142, 323, 471
AcoI YGGCCR 1 cut(s) 462
AcuI CTGAAG 1 cut(s) 354
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 2 cut(s) 283, 350
AfiI CCNNNNNNNGG 1 cut(s) 510
AflIII ACRYGT 1 cut(s) 299
AgsI TTSAA 2 cut(s) 29, 446
AjiI CACGTC 1 cut(s) 300
AjnI CCWGG 1 cut(s) 124
AluBI AGCT 5 cut(s) 82, 101, 187, 379, 400
AluI AGCT 5 cut(s) 82, 101, 187, 379, 400
Alw21I GWGCWC 1 cut(s) 84
AlwNI CAGNNNCTG 1 cut(s) 332
AoxI GGCC 2 cut(s) 127, 462
ApeKI GCWGC 2 cut(s) 144, 397
AseI ATTAAT 1 cut(s) 255
Asp700I GAANNNNTTC 2 cut(s) 186, 339
AspLEI GCGC 1 cut(s) 275
AsuHPI GGTGA 4 cut(s) 58, 67, 385, 394
BaeI ACNNNNGTAYC 2 cut(s) 340, 373
BalI TGGCCA 1 cut(s) 464
BanI GGYRCC 2 cut(s) 272, 473
BanII GRGCYC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 156, 409
BccI CCATC 3 cut(s) 37, 101, 143
BceAI ACGGC 2 cut(s) 260, 286
BcgI CGANNNNNNTGC 2 cut(s) 407, 441
BciT130I CCWGG 1 cut(s) 126
BfaI CTAG 1 cut(s) 522
BfoI RGCGCY 1 cut(s) 276
BisI GCNGC 4 cut(s) 145, 324, 398, 472
BlsI GCNGC 4 cut(s) 146, 325, 399, 473
Bme1390I CCNGG 1 cut(s) 126
BmgBI CACGTC 1 cut(s) 300
BmiI GGNNCC 2 cut(s) 274, 475
BmrFI CCNGG 1 cut(s) 126
BsaHI GRCGYC 1 cut(s) 273
BsaJI CCNNGG 3 cut(s) 124, 310, 344
BsaWI WCCGGW 1 cut(s) 476
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 1 cut(s) 510
Bse1I ACTGG 1 cut(s) 63
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 3 cut(s) 124, 310, 344
BseGI GGATG 2 cut(s) 59, 190
BseLI CCNNNNNNNGG 1 cut(s) 510
BseMII CTCAG 2 cut(s) 396, 423
BseNI ACTGG 1 cut(s) 63
BseXI GCAGC 2 cut(s) 156, 409
BshFI GGCC 2 cut(s) 129, 464
BshNI GGYRCC 2 cut(s) 272, 473
BsiHKAI GWGCWC 1 cut(s) 84
BsiSI CCGG 1 cut(s) 477
BslI CCNNNNNNNGG 1 cut(s) 510
BsnI GGCC 2 cut(s) 129, 464
Bsp1286I GDGCHC 1 cut(s) 84
Bsp1407I TGTACA 1 cut(s) 281
Bsp143I GATC 1 cut(s) 505
BspACI CCGC 3 cut(s) 142, 323, 471
BspANI GGCC 2 cut(s) 129, 464
BspCNI CTCAG 2 cut(s) 397, 424
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 274, 475
BspQI GCTCTTC 1 cut(s) 177
BspT107I GGYRCC 2 cut(s) 272, 473
BsrBI CCGCTC 1 cut(s) 323
BsrGI TGTACA 1 cut(s) 281
BsrI ACTGG 1 cut(s) 63
BssECI CCNNGG 3 cut(s) 124, 310, 344
BssMI GATC 1 cut(s) 505
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 1 cut(s) 310
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 2 cut(s) 288, 460
Bst6I CTCTTC 5 cut(s) 24, 33, 161, 174, 177
BstACI GRCGYC 1 cut(s) 273
BstAUI TGTACA 1 cut(s) 281
BstC8I GCNNGC 1 cut(s) 427
BstDEI CTNAG 2 cut(s) 405, 432
BstF5I GGATG 2 cut(s) 59, 190
BstH2I RGCGCY 1 cut(s) 276
BstHHI GCGC 1 cut(s) 275
BstKTI GATC 1 cut(s) 508
BstMBI GATC 1 cut(s) 505
BstNI CCWGG 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 124
BstV1I GCAGC 2 cut(s) 156, 409
BsuRI GGCC 2 cut(s) 129, 464
BtrI CACGTC 1 cut(s) 300
BtsCI GGATG 2 cut(s) 59, 190
BtsIMutI CAGTG 3 cut(s) 70, 429, 515
Cac8I GCNNGC 1 cut(s) 427
CaiI CAGNNNCTG 1 cut(s) 332
CciI TCATGA 1 cut(s) 247
CfoI GCGC 1 cut(s) 275
Csp6I GTAC 2 cut(s) 282, 349
CspCI CAANNNNNGTGG 2 cut(s) 340, 375
CviAII CATG 4 cut(s) 76, 131, 248, 295
CviQI GTAC 2 cut(s) 282, 349
DdeI CTNAG 2 cut(s) 405, 432
DinI GGCGCC 1 cut(s) 274
DpnI GATC 1 cut(s) 507
DpnII GATC 1 cut(s) 505
DraI TTTAAA 1 cut(s) 19
EaeI YGGCCR 1 cut(s) 462
Eam1104I CTCTTC 5 cut(s) 24, 33, 161, 174, 177
EarI CTCTTC 5 cut(s) 24, 33, 161, 174, 177
Ecl136II GAGCTC 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 310
Eco24I GRGCYC 1 cut(s) 84
Eco53kI GAGCTC 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 354
EcoICRI GAGCTC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 84
EgeI GGCGCC 1 cut(s) 274
EheI GGCGCC 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 4 cut(s) 79, 134, 251, 298
FaiI YATR 5 cut(s) 77, 110, 132, 249, 296
FatI CATG 4 cut(s) 75, 130, 247, 294
Fnu4HI GCNGC 4 cut(s) 145, 324, 398, 472
FokI GGATG 2 cut(s) 46, 177
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 4 cut(s) 145, 324, 398, 472
FspBI CTAG 1 cut(s) 522
GlaI GCGC 1 cut(s) 274
GluI GCNGC 4 cut(s) 145, 324, 398, 472
HaeII RGCGCY 1 cut(s) 276
HaeIII GGCC 2 cut(s) 129, 464
HapII CCGG 1 cut(s) 477
HhaI GCGC 1 cut(s) 275
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 4 cut(s) 79, 134, 251, 298
Hin6I GCGC 1 cut(s) 273
HinP1I GCGC 1 cut(s) 273
HinfI GANTC 2 cut(s) 72, 244
HpaII CCGG 1 cut(s) 477
HphI GGTGA 4 cut(s) 58, 67, 385, 394
Hpy166II GTNNAC 1 cut(s) 284
Hpy188I TCNGA 3 cut(s) 345, 406, 505
Hpy188III TCNNGA 3 cut(s) 248, 392, 546
Hpy8I GTNNAC 1 cut(s) 284
Hpy99I CGWCG 1 cut(s) 493
HpyAV CCTTC 2 cut(s) 364, 526
HpyCH4III ACNGT 2 cut(s) 288, 460
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 1 cut(s) 260
HpyF3I CTNAG 2 cut(s) 405, 432
HpySE526I ACGT 1 cut(s) 299
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 4 cut(s) 79, 134, 251, 298
HspAI GCGC 1 cut(s) 273
KasI GGCGCC 1 cut(s) 272
Kzo9I GATC 1 cut(s) 505
LguI GCTCTTC 1 cut(s) 177
LmnI GCTCC 2 cut(s) 79, 94
Lsp1109I GCAGC 2 cut(s) 156, 409
MaeI CTAG 1 cut(s) 522
MaeII ACGT 1 cut(s) 299
MaeIII GTNAC 2 cut(s) 55, 302
MalI GATC 1 cut(s) 507
MbiI CCGCTC 1 cut(s) 323
MboI GATC 1 cut(s) 505
MboII GAAGA 6 cut(s) 41, 50, 130, 148, 161, 194
MhlI GDGCHC 1 cut(s) 84
MlsI TGGCCA 1 cut(s) 464
MluCI AATT 1 cut(s) 7
MluNI TGGCCA 1 cut(s) 464
Mly113I GGCGCC 1 cut(s) 273
MmeI TCCRAC 1 cut(s) 509
MnlI CCTC 9 cut(s) 25, 164, 177, 228, 339, 415, 427, 447, 473
Mox20I TGGCCA 1 cut(s) 464
MroXI GAANNNNTTC 2 cut(s) 186, 339
MscI TGGCCA 1 cut(s) 464
MseI TTAA 2 cut(s) 18, 255
Msp20I TGGCCA 1 cut(s) 464
MspI CCGG 1 cut(s) 477
MspR9I CCNGG 1 cut(s) 126
MvaI CCWGG 1 cut(s) 126
NarI GGCGCC 1 cut(s) 273
NdeII GATC 1 cut(s) 505
NlaIII CATG 4 cut(s) 79, 134, 251, 298
NlaIV GGNNCC 2 cut(s) 274, 475
NmuCI GTSAC 2 cut(s) 55, 302
PagI TCATGA 1 cut(s) 247
PciSI GCTCTTC 1 cut(s) 177
PdmI GAANNNNTTC 2 cut(s) 186, 339
PfeI GAWTC 2 cut(s) 72, 244
PkrI GCNGC 4 cut(s) 146, 325, 399, 473
PluTI GGCGCC 1 cut(s) 276
PshBI ATTAAT 1 cut(s) 255
Psp124BI GAGCTC 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 2 cut(s) 274, 475
PstNI CAGNNNCTG 1 cut(s) 332
RsaI GTAC 2 cut(s) 283, 350
RsaNI GTAC 2 cut(s) 282, 349
SacI GAGCTC 1 cut(s) 84
SapI GCTCTTC 1 cut(s) 177
SaqAI TTAA 2 cut(s) 18, 255
SatI GCNGC 4 cut(s) 145, 324, 398, 472
Sau3AI GATC 1 cut(s) 505
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 1 cut(s) 84
SfoI GGCGCC 1 cut(s) 274
Sse9I AATT 1 cut(s) 7
SsiI CCGC 3 cut(s) 142, 323, 471
SspDI GGCGCC 1 cut(s) 272
SspMI CTAG 1 cut(s) 522
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 124
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 2 cut(s) 288, 460
TaiI ACGT 1 cut(s) 302
TasI AATT 1 cut(s) 7
TatI WGTACW 1 cut(s) 281
TauI GCSGC 2 cut(s) 326, 474
TfiI GAWTC 2 cut(s) 72, 244
Tru1I TTAA 2 cut(s) 18, 255
Tru9I TTAA 2 cut(s) 18, 255
TscAI CASTG 3 cut(s) 70, 436, 515
TseFI GTSAC 2 cut(s) 55, 302
TseI GCWGC 2 cut(s) 144, 397
Tsp45I GTSAC 2 cut(s) 55, 302
TspDTI ATGAA 1 cut(s) 179
TspRI CASTG 3 cut(s) 70, 436, 515
VspI ATTAAT 1 cut(s) 255
XmnI GAANNNNTTC 2 cut(s) 186, 339
XspI CTAG 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.