AT4G00230

Xylem serine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014944
Physical Location & Seq
Forward (+)
93743 .. 97467
3725 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00230.2

Sequence Viewer

Length: 2250 bp
ATGATAAGGTCAAAATGTTCTTGCCATCATCATCTACTTGTTCTTGTTATGGTTGTTCTTTGGATATCACCAAGATATGCTTCAGCCGAAGATGAACATGCAAAGGATTTCTATATCATATATTTGGGAGATCGACCGGATAATACAGAAGAAACTATCAAGACACACATAAATCTTCTATCATCTTTGAATATCAGCCAAGAAGAAGCAAAGGAAAGAAAAGTATATAGTTACACTAAAGCCTTCAACGCTTTTGCTGCCAAGCTTTCTCCACATGAAGCTAAGAAGATGATGGAGATGGAAGAAGTTGTTAGTGTATCCCGAAATCAATATCGCAAACTTCACACGACAAAATCTTGGGATTTTGTTGGACTTCCTTTGACAGCAAAAAGACATCTAAAAGCAGAGAGAGATGTTATTATTGGTGTTCTTGATACAGGAATAACGCCAGACTCAGAGAGTTTCCTAGACCATGGTCTAGGACCTCCTCCGGCTAAATGGAAAGGATCTTGTGGACCTTATAAAAATTTCACCGGATGCAACAACAAAATAATCGGCGCCAAGTACTTCAAGCACGACGGCAATGTGCCTGCCGGCGAAGTCCGATCACCGATCGATATCGACGGACATGGGACGCACACGTCATCCACCGTAGCCGGCGTTTTAGTCGCAAACGCGAGTCTCTATGGCATAGCAAACGGCACCGCCCGCGGCGCGGTTCCGTCGGCGAGGTTAGCGATGTACAAGGTTTGTTGGGCGAGATCCGGCTGTGCTGACATGGACATACTCGCCGGATTCGAAGCAGCGATTCACGACGGCGTAGAAATTATCTCCATCTCTATCGGCGGTCCAATCGCCGATTACTCTTCCGATTCGATATCCGTCGGGTCGTTTCACGCGATGAGGAAAGGAATCCTCACGGTGGCGTCCGCCGGTAACGACGGGCCTAGCTCAGGAACTGTAACGAACCATGAGCCGTGGATATTGACGGTTGCTGCAAGTGGAATCGATCGGACGTTCAAGAGCAAAATAGATCTCGGCAACGGCAAATCCTTCTCTGGGATGGGAATAAGCATGTTTAGTCCAAAAGCCAAATCGTATCCGCTTGTAAGTGGTGTTGATGCTGCTAAGAACACAGACGATAAGTACTTGGCTAGGTATTGTTTCTCTGATTCTTTGGATCGAAAGAAGGTGAAGGGAAAGGTAATGGTGTGTAGAATGGGAGGAGGTGGTGTGGAGTCTACTATCAAAAGCTATGGAGGTGCTGGTGCCATCATTGTAAGTGATCAATATCTTGACAATGCTCAGATTTTCATGGCACCTGCCACCAGTGTTAATAGCTCCGTTGGCGATATTATCTACCGATATATCAACTCCACAAGATCAGCGTCGGCTGTGATTCAGAAAACTCGGCAAGTGACAATCCCTGCTCCATTTGTTGCTTCTTTTTCATCAAGAGGTCCCAATCCGGGATCAATACGTCTTCTCAAGCCTGATATCGCTGCACCCGGGATTGATATATTGGCGGCCTTCACTCTAAAGAGATCACTGACTGGGTTAGATGGTGACACCCAGTTCTCAAAATTCACCATCCTGTCTGGCACCTCAATGGCCTGCCCTCATGTTGCTGGTGTAGCTGCGTACGTCAAGTCTTTTCATCCGGATTGGACCCCTGCTGCCATCAAATCCGCCATCATTACCTCAGCAAAACCGATAAGCCGGAGAGTGAACAAGGACGCAGAGTTTGCTTATGGAGGAGGCCAAATAAACCCACGACGAGCCGCAAGCCCTGGCTTAGTCTACGACATGGACGACATCTCCTATGTTCAGTTCTTGTGCGGCGAAGGCTACAACGCAACCACTCTAGCTCCATTGGTGGGTACACGCTCCGTGAGCTGTTCCTCCATTGTCCCTGGACTCGGCCACGATTCCCTCAACTACCCAACAATCCAACTCACGTTGAGATCCGCCAAAACGTCCACATTGGCTGTGTTCAGGCGGAGAGTCACCAACGTGGGACCACCGTCGTCGGTCTACACCGCCACCGTCCGAGCACCGAAAGGAGTAGAAATCACGGTGGAGCCACAGAGTTTGTCATTTTCAAAGGCTTCACAAAAGAGAAGCTTCAAAGTGGTGGTGAAGGCCAAACAAATGACTCCTGGGAAGATTGTGTCCGGCTTGCTCGTGTGGAAGAGCCCACGTCACTCTGTTCGTAGCCCCATTGTTATTTATAGTCCTACTTCGGATTGA

Protein Analysis

749

Amino Acids

80.33

Weight (kDa)

9.42

Isoelectric Point (pI)

35.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 38 - 115 4.6e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 137 - 569 3e-41 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00230 AT4G00230
fragaria_vesca FvH4_7g12740
malus_domestica MD02G1196900.v1.1
prunus_persica Prupe.2G151900_v2.0.a1
pyrus_communis pycom02g16020
rosa_chinensis RchiOBHm_Chr1g0351761
rosa_laevigata RLG00000028385
rosa_multiflora Rmu_sc0001933.1_g000013
rosa_roxburghii Rroxscaffold_4G00303570
rosa_rugosa Rorug01G0221200
rosa_samantha Rh1AG235200 Rh1BG205700 Rh1CG219800 Rh1DG232700
rosa_wichuraiana Rw1G020470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 522
AarI CACCTGC 1 cut(s) 1330
AasI GACNNNNNNGTC 1 cut(s) 640
Acc36I ACCTGC 1 cut(s) 1330
AccB1I GGYRCC 5 cut(s) 557, 701, 1268, 1318, 1601
AccI GTMKAC 3 cut(s) 1241, 1800, 2034
AccII CGCG 4 cut(s) 677, 711, 716, 899
AccIII TCCGGA 1 cut(s) 1660
AclWI GGATC 5 cut(s) 514, 756, 1188, 1480, 1959
AcoI YGGCCR 1 cut(s) 1921
AcsI RAATTY 2 cut(s) 526, 1583
AcuI CTGAAG 1 cut(s) 66
AcyI GRCGYC 2 cut(s) 558, 926
AfaI GTAC 5 cut(s) 566, 743, 1148, 1643, 1882
AfiI CCNNNNNNNGG 7 cut(s) 715, 922, 953, 1059, 1469, 1877, 1919
AflIII ACRYGT 1 cut(s) 639
AgsI TTSAA 6 cut(s) 190, 247, 571, 1021, 2103, 2128
AjiI CACGTC 2 cut(s) 642, 2201
AjnI CCWGG 3 cut(s) 1789, 1912, 2158
AjuI GAANNNNNNNTTGG 2 cut(s) 64, 96
AleI CACNNNNGTG 1 cut(s) 2012
AluBI AGCT 9 cut(s) 265, 281, 951, 1254, 1341, 1637, 1868, 1896, 2124
AluI AGCT 9 cut(s) 265, 281, 951, 1254, 1341, 1637, 1868, 1896, 2124
Alw21I GWGCWC 1 cut(s) 2056
Alw26I GTCTC 1 cut(s) 686
AlwI GGATC 5 cut(s) 514, 756, 1188, 1480, 1959
AlwNI CAGNNNCTG 1 cut(s) 959
Ama87I CYCGRG 1 cut(s) 1508
Aor13HI TCCGGA 1 cut(s) 1660
AoxI GGCC 6 cut(s) 944, 1527, 1611, 1759, 1921, 2142
ApeKI GCWGC 7 cut(s) 257, 803, 995, 1124, 1502, 1637, 1676
ApoI RAATTY 2 cut(s) 526, 1583
ArsI GACNNNNNNTTYG 2 cut(s) 1727, 1759
AspLEI GCGC 2 cut(s) 560, 716
AspS9I GGNCC 7 cut(s) 482, 515, 848, 944, 1460, 1668, 2018
AsuC2I CCSGG 3 cut(s) 1470, 1509, 1510
AsuHPI GGTGA 8 cut(s) 60, 523, 600, 1204, 1577, 1579, 1999, 2149
AsuII TTCGAA 1 cut(s) 798
AvaI CYCGRG 1 cut(s) 1508
AvaII GGWCC 6 cut(s) 482, 515, 848, 1460, 1668, 2018
BanI GGYRCC 5 cut(s) 557, 701, 1268, 1318, 1601
BanII GRGCYC 1 cut(s) 2198
BauI CACGAG 1 cut(s) 2183
BbsI GAAGAC 1 cut(s) 1475
Bbv12I GWGCWC 1 cut(s) 2056
BbvCI CCTCAGC 1 cut(s) 1702
BbvI GCAGC 7 cut(s) 244, 815, 982, 1111, 1489, 1624, 1663
BceAI ACGGC 5 cut(s) 595, 715, 832, 961, 1060
BciT130I CCWGG 3 cut(s) 1791, 1914, 2160
BciVI GTATCC 2 cut(s) 328, 1110
BclI TGATCA 1 cut(s) 1285
BcnI CCSGG 3 cut(s) 1470, 1509, 1510
BcoDI GTCTC 1 cut(s) 686
BfaI CTAG 5 cut(s) 467, 479, 948, 1155, 1865
BfoI RGCGCY 1 cut(s) 561
BfuAI ACCTGC 1 cut(s) 1330
BfuI GTATCC 2 cut(s) 328, 1110
BglII AGATCT 1 cut(s) 1033
BmcAI AGTACT 2 cut(s) 566, 1148
Bme1390I CCNGG 6 cut(s) 1470, 1509, 1510, 1791, 1914, 2160
Bme18I GGWCC 6 cut(s) 482, 515, 848, 1460, 1668, 2018
BmeT110I CYCGRG 1 cut(s) 1508
BmgBI CACGTC 2 cut(s) 642, 2201
BmgT120I GGNCC 7 cut(s) 482, 515, 848, 944, 1460, 1668, 2018
BmrFI CCNGG 6 cut(s) 1470, 1509, 1510, 1791, 1914, 2160
BmrI ACTGGG 2 cut(s) 1563, 1567
BmsI GCATC 2 cut(s) 527, 1111
BmuI ACTGGG 2 cut(s) 1563, 1567
BoxI GACNNNNGTC 2 cut(s) 474, 2023
BpiI GAAGAC 1 cut(s) 1475
Bpu10I CCTNAGC 2 cut(s) 952, 1702
Bpu14I TTCGAA 1 cut(s) 798
BpuEI CTTGAG 1 cut(s) 1472
BpuMI CCSGG 3 cut(s) 1470, 1509, 1510
Bsa29I ATCGAT 2 cut(s) 615, 1008
BsaBI GATNNNNATC 2 cut(s) 617, 1290
BsaHI GRCGYC 2 cut(s) 558, 926
BsaJI CCNNGG 7 cut(s) 472, 709, 977, 1508, 1789, 1912, 2159
BsaWI WCCGGW 3 cut(s) 136, 533, 1660
BsaXI ACNNNNNCTCC 6 cut(s) 1218, 1248, 1802, 1832, 1852, 1882
Bsc4I CCNNNNNNNGG 7 cut(s) 715, 922, 953, 1059, 1469, 1877, 1919
Bse118I RCCGGY 3 cut(s) 593, 656, 932
Bse1I ACTGG 3 cut(s) 1329, 1558, 1573
Bse3DI GCAATG 1 cut(s) 589
Bse8I GATNNNNATC 2 cut(s) 617, 1290
BseAI TCCGGA 1 cut(s) 1660
BseBI CCWGG 3 cut(s) 1791, 1914, 2160
BseCI ATCGAT 2 cut(s) 615, 1008
BseDI CCNNGG 7 cut(s) 472, 709, 977, 1508, 1789, 1912, 2159
BseGI GGATG 5 cut(s) 542, 644, 1068, 1590, 1657
BseJI GATNNNNATC 2 cut(s) 617, 1290
BseLI CCNNNNNNNGG 7 cut(s) 715, 922, 953, 1059, 1469, 1877, 1919
BseMI GCAATG 1 cut(s) 589
BseMII CTCAG 4 cut(s) 468, 966, 1319, 1716
BseNI ACTGG 3 cut(s) 1329, 1558, 1573
BseRI GAGGAG 3 cut(s) 477, 1239, 1770
BseXI GCAGC 7 cut(s) 244, 815, 982, 1111, 1489, 1624, 1663
BsgI GTGCAG 1 cut(s) 1488
Bsh1236I CGCG 4 cut(s) 677, 711, 716, 899
Bsh1285I CGRYCG 3 cut(s) 137, 615, 1012
BshFI GGCC 6 cut(s) 946, 1529, 1613, 1761, 1923, 2144
BshNI GGYRCC 5 cut(s) 557, 701, 1268, 1318, 1601
BshVI ATCGAT 2 cut(s) 615, 1008
BsiEI CGRYCG 3 cut(s) 137, 615, 1012
BsiHKAI GWGCWC 1 cut(s) 2056
BsiHKCI CYCGRG 1 cut(s) 1508
BsiWI CGTACG 1 cut(s) 1641
BslFI GGGAC 4 cut(s) 646, 1446, 1895, 2031
BslI CCNNNNNNNGG 7 cut(s) 715, 922, 953, 1059, 1469, 1877, 1919
BsmAI GTCTC 1 cut(s) 686
BsmFI GGGAC 4 cut(s) 646, 1446, 1895, 2031
BsnI GGCC 6 cut(s) 946, 1529, 1613, 1761, 1923, 2144
BsoBI CYCGRG 1 cut(s) 1508
Bsp119I TTCGAA 1 cut(s) 798
Bsp1286I GDGCHC 2 cut(s) 2056, 2198
Bsp13I TCCGGA 1 cut(s) 1660
Bsp1407I TGTACA 1 cut(s) 741
Bsp19I CCATGG 1 cut(s) 472
BspANI GGCC 6 cut(s) 946, 1529, 1613, 1761, 1923, 2144
BspCNI CTCAG 4 cut(s) 467, 965, 1318, 1715
BspDI ATCGAT 2 cut(s) 615, 1008
BspEI TCCGGA 1 cut(s) 1660
BspFNI CGCG 4 cut(s) 677, 711, 716, 899
BspMI ACCTGC 1 cut(s) 1330
BspPI GGATC 5 cut(s) 514, 756, 1188, 1480, 1959
BspQI GCTCTTC 1 cut(s) 2186
BspT104I TTCGAA 1 cut(s) 798
BspT107I GGYRCC 5 cut(s) 557, 701, 1268, 1318, 1601
BsrDI GCAATG 1 cut(s) 589
BsrFI RCCGGY 3 cut(s) 593, 656, 932
BsrGI TGTACA 1 cut(s) 741
BsrI ACTGG 3 cut(s) 1329, 1558, 1573
BssAI RCCGGY 3 cut(s) 593, 656, 932
BssECI CCNNGG 7 cut(s) 472, 709, 977, 1508, 1789, 1912, 2159
BssNI GRCGYC 2 cut(s) 558, 926
BssSI CACGAG 1 cut(s) 2183
BssT1I CCWWGG 1 cut(s) 472
Bst2BI CACGAG 1 cut(s) 2183
Bst2UI CCWGG 3 cut(s) 1791, 1914, 2160
Bst4CI ACNGT 7 cut(s) 652, 922, 961, 991, 2025, 2047, 2077
Bst6I CTCTTC 2 cut(s) 871, 2186
BstACI GRCGYC 2 cut(s) 558, 926
BstAPI GCANNNNNTGC 1 cut(s) 1745
BstAUI TGTACA 1 cut(s) 741
BstBI TTCGAA 1 cut(s) 798
BstC8I GCNNGC 7 cut(s) 591, 595, 658, 709, 1615, 1786, 2180
BstDEI CTNAG 7 cut(s) 282, 454, 952, 1128, 1305, 1702, 1795
BstDSI CCRYGG 3 cut(s) 472, 709, 977
BstENI CCTNNNNNAGG 1 cut(s) 951
BstF5I GGATG 5 cut(s) 542, 644, 1068, 1590, 1657
BstFNI CGCG 4 cut(s) 677, 711, 716, 899
BstH2I RGCGCY 1 cut(s) 561
BstHHI GCGC 2 cut(s) 560, 716
BstMAI GTCTC 1 cut(s) 686
BstMCI CGRYCG 3 cut(s) 137, 615, 1012
BstNI CCWGG 3 cut(s) 1791, 1914, 2160
BstNSI RCATGY 2 cut(s) 101, 1078
BstPAI GACNNNNGTC 2 cut(s) 474, 2023
BstSCI CCNGG 6 cut(s) 1468, 1507, 1508, 1789, 1912, 2158
BstUI CGCG 4 cut(s) 677, 711, 716, 899
BstV1I GCAGC 7 cut(s) 244, 815, 982, 1111, 1489, 1624, 1663
BstV2I GAAGAC 1 cut(s) 1475
BstX2I RGATCY 4 cut(s) 506, 761, 1033, 1964
BstYI RGATCY 4 cut(s) 506, 761, 1033, 1964
Bsu15I ATCGAT 2 cut(s) 615, 1008
BsuI GTATCC 2 cut(s) 328, 1110
BsuRI GGCC 6 cut(s) 946, 1529, 1613, 1761, 1923, 2144
BsuTUI ATCGAT 2 cut(s) 615, 1008
BtgI CCRYGG 3 cut(s) 472, 709, 977
BtgZI GCGATG 2 cut(s) 752, 914
BtrI CACGTC 2 cut(s) 642, 2201
BtsCI GGATG 5 cut(s) 542, 644, 1068, 1590, 1657
BtsIMutI CAGTG 2 cut(s) 1336, 1547
BveI ACCTGC 1 cut(s) 1330
Cac8I GCNNGC 7 cut(s) 591, 595, 658, 709, 1615, 1786, 2180
CaiI CAGNNNCTG 1 cut(s) 959
CfoI GCGC 2 cut(s) 560, 716
Cfr10I RCCGGY 3 cut(s) 593, 656, 932
Cfr13I GGNCC 7 cut(s) 482, 515, 848, 944, 1460, 1668, 2018
Cfr42I CCGCGG 1 cut(s) 712
Cfr9I CCCGGG 1 cut(s) 1508
ClaI ATCGAT 2 cut(s) 615, 1008
CseI GACGC 4 cut(s) 643, 915, 1377, 1745
Csp6I GTAC 5 cut(s) 565, 742, 1147, 1642, 1881
CspCI CAANNNNNGTGG 2 cut(s) 2073, 2108
CviQI GTAC 5 cut(s) 565, 742, 1147, 1642, 1881
DdeI CTNAG 7 cut(s) 282, 454, 952, 1128, 1305, 1702, 1795
DinI GGCGCC 1 cut(s) 559
DrdI GACNNNNNNGTC 1 cut(s) 640
DseDI GACNNNNNNGTC 1 cut(s) 640
EaeI YGGCCR 1 cut(s) 1921
Eam1104I CTCTTC 2 cut(s) 871, 2186
EarI CTCTTC 2 cut(s) 871, 2186
EciI GGCGGA 4 cut(s) 919, 1678, 1957, 2014
Eco130I CCWWGG 1 cut(s) 472
Eco24I GRGCYC 1 cut(s) 2198
Eco32I GATATC 4 cut(s) 66, 619, 879, 1498
Eco47I GGWCC 6 cut(s) 482, 515, 848, 1460, 1668, 2018
Eco57I CTGAAG 1 cut(s) 66
Eco88I CYCGRG 1 cut(s) 1508
EcoNI CCTNNNNNAGG 1 cut(s) 951
EcoO109I RGGNCCY 2 cut(s) 482, 1460
EcoRII CCWGG 3 cut(s) 1789, 1912, 2158
EcoRV GATATC 4 cut(s) 66, 619, 879, 1498
EcoT14I CCWWGG 1 cut(s) 472
EcoT38I GRGCYC 1 cut(s) 2198
EgeI GGCGCC 1 cut(s) 559
EheI GGCGCC 1 cut(s) 559
ErhI CCWWGG 1 cut(s) 472
FalI AAGNNNNNCTT 4 cut(s) 64, 96, 2108, 2140
FaqI GGGAC 4 cut(s) 646, 1446, 1895, 2031
FauI CCCGC 1 cut(s) 716
FbaI TGATCA 1 cut(s) 1285
FblI GTMKAC 3 cut(s) 1241, 1800, 2034
FokI GGATG 5 cut(s) 549, 631, 1075, 1577, 1644
FriOI GRGCYC 1 cut(s) 2198
FspBI CTAG 5 cut(s) 467, 479, 948, 1155, 1865
GlaI GCGC 2 cut(s) 559, 715
HaeII RGCGCY 1 cut(s) 561
HaeIII GGCC 6 cut(s) 946, 1529, 1613, 1761, 1923, 2144
HgaI GACGC 4 cut(s) 643, 915, 1377, 1745
HhaI GCGC 2 cut(s) 560, 716
Hin1I GRCGYC 2 cut(s) 558, 926
Hin6I GCGC 2 cut(s) 558, 714
HinP1I GCGC 2 cut(s) 558, 714
HindIII AAGCTT 2 cut(s) 263, 2122
HphI GGTGA 8 cut(s) 60, 523, 600, 1204, 1577, 1579, 1999, 2149
Hpy166II GTNNAC 7 cut(s) 515, 1242, 1729, 1801, 1883, 1980, 2035
Hpy188I TCNGA 9 cut(s) 457, 605, 871, 1014, 1171, 1308, 1404, 2051, 2245
Hpy188III TCNNGA 9 cut(s) 160, 321, 431, 812, 954, 1021, 1295, 1455, 1661
Hpy8I GTNNAC 7 cut(s) 515, 1242, 1729, 1801, 1883, 1980, 2035
HpyAV CCTTC 7 cut(s) 253, 1063, 1183, 1189, 1540, 1838, 2134
HpyCH4III ACNGT 7 cut(s) 652, 922, 961, 991, 2025, 2047, 2077
HpyCH4IV ACGT 8 cut(s) 641, 1016, 1480, 1644, 1958, 1976, 2013, 2200
HpyCH4V TGCA 4 cut(s) 101, 540, 998, 1505
HpyF3I CTNAG 7 cut(s) 282, 454, 952, 1128, 1305, 1702, 1795
HpySE526I ACGT 8 cut(s) 641, 1016, 1480, 1644, 1958, 1976, 2013, 2200
Hsp92I GRCGYC 2 cut(s) 558, 926
HspAI GCGC 2 cut(s) 558, 714
KasI GGCGCC 1 cut(s) 557
Kpn2I TCCGGA 1 cut(s) 1660
KroI GCCGGC 2 cut(s) 593, 656
KroNI GCCGGC 2 cut(s) 595, 658
Ksp22I TGATCA 1 cut(s) 1285
KspI CCGCGG 1 cut(s) 712
LguI GCTCTTC 1 cut(s) 2186
LmnI GCTCC 5 cut(s) 1346, 1435, 1873, 1892, 2080
Lsp1109I GCAGC 7 cut(s) 244, 815, 982, 1111, 1489, 1624, 1663
LweI GCATC 2 cut(s) 527, 1111
MaeI CTAG 5 cut(s) 467, 479, 948, 1155, 1865
MaeII ACGT 8 cut(s) 641, 1016, 1480, 1644, 1958, 1976, 2013, 2200
MaeIII GTNAC 7 cut(s) 230, 935, 961, 1417, 1565, 2005, 2201
MflI RGATCY 4 cut(s) 506, 761, 1033, 1964
MhlI GDGCHC 2 cut(s) 2056, 2198
MluCI AATT 3 cut(s) 526, 825, 1583
Mly113I GGCGCC 1 cut(s) 558
MlyI GAGTC 6 cut(s) 446, 688, 1247, 1911, 2013, 2149
MmeI TCCRAC 2 cut(s) 349, 1975
MroI TCCGGA 1 cut(s) 1660
MroNI GCCGGC 2 cut(s) 593, 656
MseI TTAA 1 cut(s) 1335
MslI CAYNNNNRTG 2 cut(s) 1607, 2012
MspA1I CMGCKG 1 cut(s) 711
MspR9I CCNGG 6 cut(s) 1470, 1509, 1510, 1791, 1914, 2160
MvaI CCWGG 3 cut(s) 1791, 1914, 2160
MvnI CGCG 4 cut(s) 677, 711, 716, 899
NaeI GCCGGC 2 cut(s) 595, 658
NarI GGCGCC 1 cut(s) 558
NciI CCSGG 3 cut(s) 1470, 1509, 1510
NcoI CCATGG 1 cut(s) 472
NgoMIV GCCGGC 2 cut(s) 593, 656
NmeAIII GCCGAG 3 cut(s) 1017, 1390, 1899
NmuCI GTSAC 4 cut(s) 1417, 1565, 2005, 2201
NspI RCATGY 2 cut(s) 101, 1078
NspV TTCGAA 1 cut(s) 798
OliI CACNNNNGTG 1 cut(s) 2012
PaqCI CACCTGC 1 cut(s) 1330
PciSI GCTCTTC 1 cut(s) 2186
PcsI WCGNNNNNNNCGW 3 cut(s) 795, 896, 1809
PdiI GCCGGC 2 cut(s) 595, 658
PfeI GAWTC 8 cut(s) 795, 808, 872, 912, 1005, 1172, 1399, 1928
Pfl23II CGTACG 1 cut(s) 1641
PfoI TCCNGGA 1 cut(s) 1468
Ple19I CGATCG 2 cut(s) 615, 1012
PleI GAGTC 6 cut(s) 446, 687, 1246, 1911, 2012, 2149
PluTI GGCGCC 1 cut(s) 561
PpsI GAGTC 6 cut(s) 446, 687, 1246, 1911, 2012, 2149
PpuMI RGGWCCY 2 cut(s) 482, 1460
PshAI GACNNNNGTC 2 cut(s) 474, 2023
PsiI TTATAA 1 cut(s) 522
Psp5II RGGWCCY 2 cut(s) 482, 1460
Psp6I CCWGG 3 cut(s) 1789, 1912, 2158
PspGI CCWGG 3 cut(s) 1789, 1912, 2158
PspLI CGTACG 1 cut(s) 1641
PspPI GGNCC 7 cut(s) 482, 515, 848, 944, 1460, 1668, 2018
PspPPI RGGWCCY 2 cut(s) 482, 1460
PstNI CAGNNNCTG 1 cut(s) 959
PsuI RGATCY 4 cut(s) 506, 761, 1033, 1964
PvuI CGATCG 2 cut(s) 615, 1012
RsaI GTAC 5 cut(s) 566, 743, 1148, 1643, 1882
RsaNI GTAC 5 cut(s) 565, 742, 1147, 1642, 1881
RseI CAYNNNNRTG 2 cut(s) 1607, 2012
SacII CCGCGG 1 cut(s) 712
SapI GCTCTTC 1 cut(s) 2186
SaqAI TTAA 1 cut(s) 1335
Sau96I GGNCC 7 cut(s) 482, 515, 848, 944, 1460, 1668, 2018
ScaI AGTACT 2 cut(s) 566, 1148
SchI GAGTC 6 cut(s) 446, 688, 1247, 1911, 2013, 2149
ScrFI CCNGG 6 cut(s) 1470, 1509, 1510, 1791, 1914, 2160
SduI GDGCHC 2 cut(s) 2056, 2198
SfaNI GCATC 2 cut(s) 527, 1111
SfoI GGCGCC 1 cut(s) 559
Sfr303I CCGCGG 1 cut(s) 712
SfuI TTCGAA 1 cut(s) 798
SgrBI CCGCGG 1 cut(s) 712
SinI GGWCC 6 cut(s) 482, 515, 848, 1460, 1668, 2018
SmaI CCCGGG 1 cut(s) 1510
SmiMI CAYNNNNRTG 2 cut(s) 1607, 2012
SmlI CTYRAG 1 cut(s) 1487
SmoI CTYRAG 1 cut(s) 1487
Sse9I AATT 3 cut(s) 526, 825, 1583
SspDI GGCGCC 1 cut(s) 557
SspMI CTAG 5 cut(s) 467, 479, 948, 1155, 1865
StyD4I CCNGG 6 cut(s) 1468, 1507, 1508, 1789, 1912, 2158
StyI CCWWGG 1 cut(s) 472
TaaI ACNGT 7 cut(s) 652, 922, 961, 991, 2025, 2047, 2077
TaiI ACGT 8 cut(s) 644, 1019, 1483, 1647, 1961, 1979, 2016, 2203
TaqI TCGA 7 cut(s) 133, 615, 621, 798, 875, 1008, 1183
TaqII GACCGA 1 cut(s) 2020
TasI AATT 3 cut(s) 526, 825, 1583
TatI WGTACW 3 cut(s) 564, 741, 1146
TauI GCSGC 4 cut(s) 714, 1529, 1784, 1842
TfiI GAWTC 8 cut(s) 795, 808, 872, 912, 1005, 1172, 1399, 1928
Tru1I TTAA 1 cut(s) 1335
Tru9I TTAA 1 cut(s) 1335
TscAI CASTG 2 cut(s) 1336, 1554
TseFI GTSAC 4 cut(s) 1417, 1565, 2005, 2201
TseI GCWGC 7 cut(s) 257, 803, 995, 1124, 1502, 1637, 1676
Tsp45I GTSAC 4 cut(s) 1417, 1565, 2005, 2201
TspDTI ATGAA 5 cut(s) 108, 291, 1303, 1440, 1646
TspGWI ACGGA 5 cut(s) 639, 711, 871, 1333, 1879
TspMI CCCGGG 1 cut(s) 1508
TspRI CASTG 2 cut(s) 1336, 1554
VpaK11BI GGWCC 6 cut(s) 482, 515, 848, 1460, 1668, 2018
XagI CCTNNNNNAGG 1 cut(s) 951
XapI RAATTY 2 cut(s) 526, 1583
XceI RCATGY 2 cut(s) 101, 1078
XmaI CCCGGG 1 cut(s) 1508
XmiI GTMKAC 3 cut(s) 1241, 1800, 2034
XspI CTAG 5 cut(s) 467, 479, 948, 1155, 1865
ZrmI AGTACT 2 cut(s) 566, 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.