pycom02g16020

Xylem serine proteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014944
Physical Location & Seq
Reverse (-)
13078095 .. 13081763
3669 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g16020.7

Sequence Viewer

Length: 1308 bp
ATGTATAAAAACAAGAGGAACCAATTTGCGAGGCAGCTGAGTGCAAACCCAACACTAAATCTTGCAAGCATGTGGAGCAAGAAAATTAGTTCTTCCTTTCTTCTTCTTTTTCTTCTTCATTTGATCTTAGTAGGCGTTGTTTGTGTAAAAGGAGATGACCAAAAGTCCATGGTTTATAGCTATAAAAAGAGCTTCGATGCCTTTGCTGCCAAGCTTTCTAGCAATGAAGCCAAAAAATTAGAAAGAATGGACGAGGTTCTCTCCGTGATTCCTAATAGGTATCACAGGCTACACACAACAAGATCATGGGATTTCATTGGGTTACCACAAAAAGCTAGACGAAATTTGAAGACTGAAAGTGACATAATTGTTGGTCTCCTTGATACAGGTGCCAATTACTTCAAGCTTGATGGCAATCCAGACCCAGCTGACATCTTATCTCCCTTAGATGTGAACGGCCATGGGACTCATACCTCATCAACCTTAGCAGGCAACCAGGTCCCCAACGCCAACCTCTTCGGCCTAGCCAAGGGCACTGCACGAGGTGCAGTGCCCTCGGCTAGGGTGGCCATGTACAAAGTATGCTGGGCTAGCAGTGGCTGTTCAGACATGGACATCCTAGCAGCGTATGATGCTGCCATACATGACGGCGTCGATGTCATATCAGTTTCTATTGGTGGAGGGGGTTCAAGTTATGTGTCGGACATTATAGCAATAGGTGCATTTCATGCCATTAAGAAGGGTATTATTACTGTGGCCTCAGCTGGAAATGATGGACCAAATTTGGGAAGTGTTCTTAATCATGCACCATGGTTGGTAACTGTGGCTGCCAGTGGGATTGATAGGGATTTCATTAGCAAAGTTCATCTGGGCAATGGAAAGGATGTCTCAGGGGTTGGAGTGAGCATGTTCAGTCCAAAGAAAAACTTATACCCTATTGTTAGTGGGGTTGATGTAGCACAAAGCTCTCAGACCAAGGAAGGTGCAAGGTTCTGTGTTCAGGATACATTAGACCCTGGAAAGGTAAAGGGCAAGCTTGTTTTCTGCAAACTCGGATCTTGGGGCGCAGATTCTGTGGTTAAAGGAATTGGAGGGGCTGGCATTATAATTGAAGATGATCAGTTTCTTGATGCTGCTCAAATATTCATGGCTCCGGGAACCATGGTCAATTCTACAATCGGGCATATCATAGATATCACCATCAGCAGTGATATACAAGTCTCAACAAACAAAGAAACGTGCTCCGTTTATTGCTTCGTTCTCCTCCCGGGGTCCAAATCAAGGATCCAACCGCATCCTCAAGCCTGA

Protein Analysis

436

Amino Acids

46.32

Weight (kDa)

8.79

Isoelectric Point (pI)

22.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00230 AT4G00230
fragaria_vesca FvH4_7g12740
malus_domestica MD02G1196900.v1.1
prunus_persica Prupe.2G151900_v2.0.a1
pyrus_communis pycom02g16020
rosa_chinensis RchiOBHm_Chr1g0351761
rosa_laevigata RLG00000028385
rosa_multiflora Rmu_sc0001933.1_g000013
rosa_roxburghii Rroxscaffold_4G00303570
rosa_rugosa Rorug01G0221200
rosa_samantha Rh1AG235200 Rh1BG205700 Rh1CG219800 Rh1DG232700
rosa_wichuraiana Rw1G020470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1106
AccB1I GGYRCC 1 cut(s) 389
AciI CCGC 1 cut(s) 1292
AclWI GGATC 3 cut(s) 1063, 1279, 1292
AcoI YGGCCR 2 cut(s) 457, 567
AcsI RAATTY 2 cut(s) 343, 781
AcyI GRCGYC 1 cut(s) 651
AdeI CACNNNGTG 1 cut(s) 545
AfaI GTAC 1 cut(s) 575
AfiI CCNNNNNNNGG 5 cut(s) 529, 561, 785, 1021, 1281
AgsI TTSAA 4 cut(s) 349, 403, 690, 1112
AhdI GACNNNNNGTC 1 cut(s) 163
AjnI CCWGG 2 cut(s) 495, 1015
Alw21I GWGCWC 1 cut(s) 1244
Alw26I GTCTC 3 cut(s) 380, 892, 1225
AlwI GGATC 3 cut(s) 1063, 1279, 1292
AlwNI CAGNNNCTG 2 cut(s) 600, 1073
Ama87I CYCGRG 1 cut(s) 1267
AoxI GGCC 4 cut(s) 457, 520, 567, 756
ApeKI GCWGC 6 cut(s) 34, 206, 623, 635, 827, 1133
ApoI RAATTY 2 cut(s) 343, 781
AspLEI GCGC 1 cut(s) 1067
AspS9I GGNCC 3 cut(s) 499, 776, 1272
AsuC2I CCSGG 3 cut(s) 1155, 1268, 1269
AsuHPI GGTGA 1 cut(s) 1189
AsuNHI GCTAGC 1 cut(s) 590
AvaI CYCGRG 1 cut(s) 1267
AvaII GGWCC 3 cut(s) 499, 776, 1272
BaeGI GKGCMC 2 cut(s) 536, 555
BalI TGGCCA 1 cut(s) 569
BamHI GGATCC 1 cut(s) 1284
BanI GGYRCC 1 cut(s) 389
BauI CACGAG 1 cut(s) 540
BbsI GAAGAC 1 cut(s) 356
Bbv12I GWGCWC 1 cut(s) 1244
BbvCI CCTCAGC 1 cut(s) 760
BbvI GCAGC 6 cut(s) 46, 193, 622, 635, 814, 1120
BccI CCATC 3 cut(s) 404, 767, 1208
BceAI ACGGC 2 cut(s) 472, 664
BcgI CGANNNNNNTGC 4 cut(s) 185, 219, 537, 571
BciT130I CCWGG 2 cut(s) 497, 1017
BciVI GTATCC 1 cut(s) 997
BclI TGATCA 1 cut(s) 1117
BcnI CCSGG 3 cut(s) 1155, 1268, 1269
BcoDI GTCTC 3 cut(s) 380, 892, 1225
BfaI CTAG 6 cut(s) 219, 336, 524, 561, 591, 620
BfuI GTATCC 1 cut(s) 997
BisI GCNGC 6 cut(s) 35, 207, 624, 636, 828, 1134
BlsI GCNGC 6 cut(s) 36, 208, 625, 637, 829, 1135
Bme1390I CCNGG 5 cut(s) 497, 1017, 1155, 1268, 1269
Bme18I GGWCC 3 cut(s) 499, 776, 1272
BmeRI GACNNNNNGTC 1 cut(s) 163
BmeT110I CYCGRG 1 cut(s) 1267
BmgT120I GGNCC 3 cut(s) 499, 776, 1272
BmiI GGNNCC 7 cut(s) 20, 391, 501, 1152, 1159, 1273, 1286
BmrFI CCNGG 5 cut(s) 497, 1017, 1155, 1268, 1269
BmsI GCATC 4 cut(s) 187, 622, 1120, 1303
BmtI GCTAGC 1 cut(s) 594
BpiI GAAGAC 1 cut(s) 356
BplI GAGNNNNNCTC 2 cut(s) 245, 277
Bpu10I CCTNAGC 2 cut(s) 484, 760
BpuEI CTTGAG 1 cut(s) 1284
BpuMI CCSGG 3 cut(s) 1155, 1268, 1269
BsaBI GATNNNNATC 1 cut(s) 414
BsaHI GRCGYC 1 cut(s) 651
BsaI GGTCTC 1 cut(s) 380
Bsc4I CCNNNNNNNGG 5 cut(s) 529, 561, 785, 1021, 1281
Bse1I ACTGG 1 cut(s) 831
Bse3DI GCAATG 2 cut(s) 229, 880
Bse8I GATNNNNATC 1 cut(s) 414
BseBI CCWGG 2 cut(s) 497, 1017
BseGI GGATG 3 cut(s) 615, 889, 1294
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 5 cut(s) 529, 561, 785, 1021, 1281
BseMI GCAATG 2 cut(s) 229, 880
BseMII CTCAG 4 cut(s) 29, 774, 903, 983
BseNI ACTGG 1 cut(s) 831
BseRI GAGGAG 1 cut(s) 1253
BseSI GKGCMC 2 cut(s) 536, 555
BseXI GCAGC 6 cut(s) 46, 193, 622, 635, 814, 1120
BseYI CCCAGC 2 cut(s) 424, 585
BsgI GTGCAG 2 cut(s) 522, 567
BshFI GGCC 4 cut(s) 459, 522, 569, 758
BshNI GGYRCC 1 cut(s) 389
BsiHKAI GWGCWC 1 cut(s) 1244
BsiHKCI CYCGRG 1 cut(s) 1267
BsiSI CCGG 2 cut(s) 1154, 1268
BslFI GGGAC 2 cut(s) 478, 485
BslI CCNNNNNNNGG 5 cut(s) 529, 561, 785, 1021, 1281
BsmAI GTCTC 3 cut(s) 380, 892, 1225
BsmFI GGGAC 2 cut(s) 478, 485
BsnI GGCC 4 cut(s) 459, 522, 569, 758
Bso31I GGTCTC 1 cut(s) 380
BsoBI CYCGRG 1 cut(s) 1267
Bsp1286I GDGCHC 3 cut(s) 536, 555, 1244
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 5 cut(s) 123, 302, 1055, 1117, 1284
Bsp19I CCATGG 4 cut(s) 168, 460, 809, 1161
BspACI CCGC 1 cut(s) 1292
BspANI GGCC 4 cut(s) 459, 522, 569, 758
BspCNI CTCAG 4 cut(s) 30, 773, 902, 982
BspLI GGNNCC 7 cut(s) 20, 391, 501, 1152, 1159, 1273, 1286
BspOI GCTAGC 1 cut(s) 594
BspPI GGATC 3 cut(s) 1063, 1279, 1292
BspT107I GGYRCC 1 cut(s) 389
BspTNI GGTCTC 1 cut(s) 380
BsrDI GCAATG 2 cut(s) 229, 880
BsrGI TGTACA 1 cut(s) 573
BsrI ACTGG 1 cut(s) 831
BssMI GATC 5 cut(s) 123, 302, 1055, 1117, 1284
BssNI GRCGYC 1 cut(s) 651
BssSI CACGAG 1 cut(s) 540
BssT1I CCWWGG 6 cut(s) 168, 460, 528, 809, 975, 1161
Bst2BI CACGAG 1 cut(s) 540
Bst2UI CCWGG 2 cut(s) 497, 1017
Bst4CI ACNGT 2 cut(s) 754, 823
Bst6I CTCTTC 1 cut(s) 521
BstACI GRCGYC 1 cut(s) 651
BstAPI GCANNNNNTGC 3 cut(s) 545, 719, 728
BstAUI TGTACA 1 cut(s) 573
BstC8I GCNNGC 5 cut(s) 67, 490, 592, 1034, 1099
BstDEI CTNAG 7 cut(s) 38, 127, 445, 484, 760, 889, 969
BstDSI CCRYGG 4 cut(s) 168, 460, 809, 1161
BstEII GGTNACC 1 cut(s) 321
BstENI CCTNNNNNAGG 2 cut(s) 527, 559
BstF5I GGATG 3 cut(s) 615, 889, 1294
BstHHI GCGC 1 cut(s) 1067
BstKTI GATC 5 cut(s) 126, 305, 1058, 1120, 1287
BstMAI GTCTC 3 cut(s) 380, 892, 1225
BstMBI GATC 5 cut(s) 123, 302, 1055, 1117, 1284
BstMWI GCNNNNNNNGC 8 cut(s) 75, 206, 545, 566, 591, 632, 719, 728
BstNI CCWGG 2 cut(s) 497, 1017
BstNSI RCATGY 2 cut(s) 73, 910
BstPI GGTNACC 1 cut(s) 321
BstSCI CCNGG 5 cut(s) 495, 1015, 1153, 1266, 1267
BstSLI GKGCMC 2 cut(s) 536, 555
BstV1I GCAGC 6 cut(s) 46, 193, 622, 635, 814, 1120
BstV2I GAAGAC 1 cut(s) 356
BstX2I RGATCY 2 cut(s) 1055, 1284
BstYI RGATCY 2 cut(s) 1055, 1284
BsuI GTATCC 1 cut(s) 997
BsuRI GGCC 4 cut(s) 459, 522, 569, 758
BtgI CCRYGG 4 cut(s) 168, 460, 809, 1161
BtsCI GGATG 3 cut(s) 615, 889, 1294
BtsI GCAGTG 4 cut(s) 534, 555, 601, 1213
BtsIMutI CAGTG 5 cut(s) 534, 555, 601, 838, 1213
Cac8I GCNNGC 5 cut(s) 67, 490, 592, 1034, 1099
CaiI CAGNNNCTG 2 cut(s) 600, 1073
CfoI GCGC 1 cut(s) 1067
Cfr13I GGNCC 3 cut(s) 499, 776, 1272
Cfr9I CCCGGG 1 cut(s) 1267
CseI GACGC 1 cut(s) 640
CsiI ACCWGGT 1 cut(s) 495
Csp6I GTAC 1 cut(s) 574
CviQI GTAC 1 cut(s) 574
DdeI CTNAG 7 cut(s) 38, 127, 445, 484, 760, 889, 969
DpnI GATC 5 cut(s) 125, 304, 1057, 1119, 1286
DpnII GATC 5 cut(s) 123, 302, 1055, 1117, 1284
DraIII CACNNNGTG 1 cut(s) 545
DriI GACNNNNNGTC 1 cut(s) 163
EaeI YGGCCR 2 cut(s) 457, 567
Eam1104I CTCTTC 1 cut(s) 521
Eam1105I GACNNNNNGTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 521
Eco130I CCWWGG 6 cut(s) 168, 460, 528, 809, 975, 1161
Eco31I GGTCTC 1 cut(s) 380
Eco32I GATATC 1 cut(s) 1195
Eco47I GGWCC 3 cut(s) 499, 776, 1272
Eco88I CYCGRG 1 cut(s) 1267
Eco91I GGTNACC 1 cut(s) 321
EcoNI CCTNNNNNAGG 2 cut(s) 527, 559
EcoO109I RGGNCCY 1 cut(s) 499
EcoO65I GGTNACC 1 cut(s) 321
EcoRII CCWGG 2 cut(s) 495, 1015
EcoRV GATATC 1 cut(s) 1195
EcoT14I CCWWGG 6 cut(s) 168, 460, 528, 809, 975, 1161
ErhI CCWWGG 6 cut(s) 168, 460, 528, 809, 975, 1161
FalI AAGNNNNNCTT 2 cut(s) 911, 943
FaqI GGGAC 2 cut(s) 478, 485
FbaI TGATCA 1 cut(s) 1117
Fnu4HI GCNGC 6 cut(s) 35, 207, 624, 636, 828, 1134
FokI GGATG 3 cut(s) 602, 896, 1281
Fsp4HI GCNGC 6 cut(s) 35, 207, 624, 636, 828, 1134
FspBI CTAG 6 cut(s) 219, 336, 524, 561, 591, 620
GlaI GCGC 1 cut(s) 1066
GluI GCNGC 6 cut(s) 35, 207, 624, 636, 828, 1134
GsaI CCCAGC 2 cut(s) 428, 589
HaeIII GGCC 4 cut(s) 459, 522, 569, 758
HapII CCGG 2 cut(s) 1154, 1268
HgaI GACGC 1 cut(s) 640
HhaI GCGC 1 cut(s) 1067
Hin1I GRCGYC 1 cut(s) 651
Hin6I GCGC 1 cut(s) 1065
HinP1I GCGC 1 cut(s) 1065
HindIII AAGCTT 3 cut(s) 212, 404, 1034
HinfI GANTC 3 cut(s) 268, 466, 1070
HpaII CCGG 2 cut(s) 1154, 1268
HphI GGTGA 1 cut(s) 1189
Hpy166II GTNNAC 1 cut(s) 454
Hpy188I TCNGA 4 cut(s) 607, 703, 972, 1055
Hpy188III TCNNGA 3 cut(s) 419, 1001, 1127
Hpy8I GTNNAC 1 cut(s) 454
Hpy99I CGWCG 1 cut(s) 656
HpyAV CCTTC 2 cut(s) 733, 974
HpyCH4III ACNGT 2 cut(s) 754, 823
HpyCH4IV ACGT 1 cut(s) 1238
HpyCH4V TGCA 8 cut(s) 44, 65, 539, 548, 722, 806, 986, 1047
HpyF10VI GCNNNNNNNGC 8 cut(s) 75, 206, 545, 566, 591, 632, 719, 728
HpyF3I CTNAG 7 cut(s) 38, 127, 445, 484, 760, 889, 969
HpySE526I ACGT 1 cut(s) 1238
Hsp92I GRCGYC 1 cut(s) 651
HspAI GCGC 1 cut(s) 1065
Ksp22I TGATCA 1 cut(s) 1117
Kzo9I GATC 5 cut(s) 123, 302, 1055, 1117, 1284
LmnI GCTCC 3 cut(s) 75, 1156, 1247
Lsp1109I GCAGC 6 cut(s) 46, 193, 622, 635, 814, 1120
LweI GCATC 4 cut(s) 187, 622, 1120, 1303
MabI ACCWGGT 1 cut(s) 495
MaeI CTAG 6 cut(s) 219, 336, 524, 561, 591, 620
MaeII ACGT 1 cut(s) 1238
MaeIII GTNAC 3 cut(s) 321, 359, 817
MalI GATC 5 cut(s) 125, 304, 1057, 1119, 1286
MboI GATC 5 cut(s) 123, 302, 1055, 1117, 1284
MboII GAAGA 8 cut(s) 84, 92, 95, 104, 107, 361, 508, 1124
MflI RGATCY 2 cut(s) 1055, 1284
MhlI GDGCHC 3 cut(s) 536, 555, 1244
MlsI TGGCCA 1 cut(s) 569
MluNI TGGCCA 1 cut(s) 569
MlyI GAGTC 1 cut(s) 460
MmeI TCCRAC 2 cut(s) 681, 877
Mox20I TGGCCA 1 cut(s) 569
MscI TGGCCA 1 cut(s) 569
MseI TTAA 3 cut(s) 735, 798, 1080
Msp20I TGGCCA 1 cut(s) 569
MspA1I CMGCKG 3 cut(s) 37, 428, 764
MspI CCGG 2 cut(s) 1154, 1268
MspR9I CCNGG 5 cut(s) 497, 1017, 1155, 1268, 1269
MvaI CCWGG 2 cut(s) 497, 1017
MwoI GCNNNNNNNGC 8 cut(s) 75, 206, 545, 566, 591, 632, 719, 728
NciI CCSGG 3 cut(s) 1155, 1268, 1269
NcoI CCATGG 4 cut(s) 168, 460, 809, 1161
NdeII GATC 5 cut(s) 123, 302, 1055, 1117, 1284
NheI GCTAGC 1 cut(s) 590
NlaIV GGNNCC 7 cut(s) 20, 391, 501, 1152, 1159, 1273, 1286
NmeAIII GCCGAG 1 cut(s) 536
NmuCI GTSAC 1 cut(s) 359
NspI RCATGY 2 cut(s) 73, 910
PfeI GAWTC 2 cut(s) 268, 1070
PflFI GACNNNGTC 1 cut(s) 650
PfoI TCCNGGA 1 cut(s) 1153
PkrI GCNGC 6 cut(s) 36, 208, 625, 637, 829, 1135
PleI GAGTC 1 cut(s) 460
PpsI GAGTC 1 cut(s) 460
PpuMI RGGWCCY 1 cut(s) 499
PsiI TTATAA 1 cut(s) 1106
Psp5II RGGWCCY 1 cut(s) 499
Psp6I CCWGG 2 cut(s) 495, 1015
PspEI GGTNACC 1 cut(s) 321
PspFI CCCAGC 2 cut(s) 424, 585
PspGI CCWGG 2 cut(s) 495, 1015
PspN4I GGNNCC 7 cut(s) 20, 391, 501, 1152, 1159, 1273, 1286
PspPI GGNCC 3 cut(s) 499, 776, 1272
PspPPI RGGWCCY 1 cut(s) 499
PstNI CAGNNNCTG 2 cut(s) 600, 1073
PsuI RGATCY 2 cut(s) 1055, 1284
PsyI GACNNNGTC 1 cut(s) 650
PvuII CAGCTG 3 cut(s) 37, 428, 764
RsaI GTAC 1 cut(s) 575
RsaNI GTAC 1 cut(s) 574
SaqAI TTAA 3 cut(s) 735, 798, 1080
SatI GCNGC 6 cut(s) 35, 207, 624, 636, 828, 1134
Sau3AI GATC 5 cut(s) 123, 302, 1055, 1117, 1284
Sau96I GGNCC 3 cut(s) 499, 776, 1272
SchI GAGTC 1 cut(s) 460
ScrFI CCNGG 5 cut(s) 497, 1017, 1155, 1268, 1269
SduI GDGCHC 3 cut(s) 536, 555, 1244
SexAI ACCWGGT 1 cut(s) 495
SfaNI GCATC 4 cut(s) 187, 622, 1120, 1303
SinI GGWCC 3 cut(s) 499, 776, 1272
SmaI CCCGGG 1 cut(s) 1269
SmlI CTYRAG 1 cut(s) 1299
SmoI CTYRAG 1 cut(s) 1299
SsiI CCGC 1 cut(s) 1292
SspI AATATT 1 cut(s) 1143
SspMI CTAG 6 cut(s) 219, 336, 524, 561, 591, 620
StyD4I CCNGG 5 cut(s) 495, 1015, 1153, 1266, 1267
StyI CCWWGG 6 cut(s) 168, 460, 528, 809, 975, 1161
TaaI ACNGT 2 cut(s) 754, 823
TaiI ACGT 1 cut(s) 1241
TaqI TCGA 2 cut(s) 195, 654
TatI WGTACW 1 cut(s) 573
TfiI GAWTC 2 cut(s) 268, 1070
Tru1I TTAA 3 cut(s) 735, 798, 1080
Tru9I TTAA 3 cut(s) 735, 798, 1080
TscAI CASTG 5 cut(s) 541, 555, 601, 838, 1213
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 6 cut(s) 34, 206, 623, 635, 827, 1133
Tsp45I GTSAC 1 cut(s) 359
TspDTI ATGAA 7 cut(s) 107, 240, 304, 716, 841, 854, 1135
TspGWI ACGGA 2 cut(s) 253, 1234
TspMI CCCGGG 1 cut(s) 1267
TspRI CASTG 5 cut(s) 541, 555, 601, 838, 1213
Tth111I GACNNNGTC 1 cut(s) 650
VpaK11BI GGWCC 3 cut(s) 499, 776, 1272
XagI CCTNNNNNAGG 2 cut(s) 527, 559
XapI RAATTY 2 cut(s) 343, 781
XceI RCATGY 2 cut(s) 73, 910
XmaI CCCGGG 1 cut(s) 1267
XspI CTAG 6 cut(s) 219, 336, 524, 561, 591, 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.