Rh1AG235200

Xylem serine proteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0014944
Physical Location & Seq
Forward (+)
44581274 .. 44584048
2775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG235200.1

Sequence Viewer

Length: 1191 bp
ATGGGGATTGGAGTGAGCACATTTAATCCAAATAAGACGTTGTACCCTCTTGTTAGTGGGGCTGATGTAGCACAAAGCTCGGAAAGCAAAGAAAATGCAAGGTTCTGTGCTGAGGACTCATTAGACCCTAAAAAAGTAAAGGGAAAGATTGTTTTCTGCCAATTTAGATCTTGGGGTACAGATTCTGTCATTAAAGGAAATGGAGGGGTTGGTACCATTGTTGAAAATGATCAATTTCTCGATGCTGCTCAAATATTCATGGCACCAGGAACCATGGTTAACTCTACAATTGGCGAAAGCATTGATGCCTATATACATTCAGAAAGATCACCATCAGCAGTTATATTCAAGTCACAAGAAGCACAAAAACGTGCTCCATATGTTGCTTCATTTTCATCCAGGGGTCCAAATCCGGGATCAGAACACATTCTAAAGCCTGATATTGCAGCACCTGGGATTGACATTTTGGCATCTTACACTCCATTGAAGTCACTTACTGGGTTGGATGGTGACACACAATACTCAGAATTCACACTCATGTCTGGAACTTCAATGGCATGTCCACATGTTGCTGGTGTAGCAGCATATGTTAAGTCATTTCACCCGAATTGGTCTCAAGCTGCAATAAGATCAGCCATCATGACCACAGCAACACCGATGAGCCATAGAGTGAACACAGAAGCAGAATTCGCCTATGGTGCTGGTCAAGTGAACCCGAGAAAAGCTGTGAATCCAGGCTTAATCTATGACATGGATGAAATGTCATACATTCAGTTCCTATGCCGTGAAGGCTATAGTGGCTCCTCATTAGCCCCATTAGTTGGCTCAAAGTCCATAAACTGCTCCTCGTTGATTCCTGGACTCGGCTACGATGCTCTCAACTATCCCACCATCCAGCTCAGCTTGAAAAGCAGCAAGCAACCGACAGTAGCTGTTTTCAGAAGAACTGTCACTAATGTTGGTCCTGCTAGTTCCACATACAATGCCACCATCAGAGCACCAAAGGGAGTGGAAATCACTGTCAAACCCATGAGTCTGACTTTCTCTCATGCTTTGCAAAAGAGGAGCTTCCAAGTTGTGGTGAAGGCGAATCCGATAGACAGTATGCAAATGGTGTCTGGCTCGCTCGGATGGAAAAGCTCTAGGTACACCGTGAGGAGTCCCATTGTGGTTTACAGTCCAGGAAATTAA

Protein Analysis

396

Amino Acids

42.41

Weight (kDa)

9.02

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 123 - 235 1.3e-22 Subtilase family
fn3_6 PF17766 293 - 391 1.6e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00230 AT4G00230
fragaria_vesca FvH4_7g12740
malus_domestica MD02G1196900.v1.1
prunus_persica Prupe.2G151900_v2.0.a1
pyrus_communis pycom02g16020
rosa_chinensis RchiOBHm_Chr1g0351761
rosa_laevigata RLG00000028385
rosa_multiflora Rmu_sc0001933.1_g000013
rosa_roxburghii Rroxscaffold_4G00303570
rosa_rugosa Rorug01G0221200
rosa_samantha Rh1AG235200 Rh1BG205700 Rh1CG219800 Rh1DG232700
rosa_wichuraiana Rw1G020470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 212
AccB1I GGYRCC 2 cut(s) 212, 262
AccB7I CCANNNNNTGG 2 cut(s) 821, 1078
AclWI GGATC 1 cut(s) 424
AcsI RAATTY 2 cut(s) 527, 686
AfaI GTAC 4 cut(s) 44, 178, 214, 1148
AfiI CCNNNNNNNGG 4 cut(s) 413, 821, 863, 1078
AflIII ACRYGT 1 cut(s) 565
AgsI TTSAA 5 cut(s) 224, 349, 487, 552, 907
AjnI CCWGG 6 cut(s) 265, 398, 451, 733, 856, 1180
AluBI AGCT 8 cut(s) 78, 620, 725, 898, 903, 932, 1068, 1140
AluI AGCT 8 cut(s) 78, 620, 725, 898, 903, 932, 1068, 1140
Alw21I GWGCWC 3 cut(s) 20, 376, 1000
Alw26I GTCTC 1 cut(s) 618
AlwI GGATC 1 cut(s) 424
AlwNI CAGNNNCTG 3 cut(s) 185, 452, 932
Ama87I CYCGRG 1 cut(s) 715
ApeKI GCWGC 5 cut(s) 245, 446, 581, 620, 912
ApoI RAATTY 2 cut(s) 527, 686
Asp700I GAANNNNTTC 1 cut(s) 426
Asp718I GGTACC 1 cut(s) 212
AspS9I GGNCC 2 cut(s) 404, 962
AsuC2I CCSGG 1 cut(s) 414
AsuHPI GGTGA 4 cut(s) 321, 521, 593, 1093
AvaI CYCGRG 1 cut(s) 715
AvaII GGWCC 2 cut(s) 404, 962
BaeI ACNNNNGTAYC 2 cut(s) 204, 237
BanI GGYRCC 2 cut(s) 212, 262
Bbv12I GWGCWC 3 cut(s) 20, 376, 1000
BbvCI CCTCAGC 1 cut(s) 111
BbvI GCAGC 5 cut(s) 232, 458, 593, 607, 924
BccI CCATC 6 cut(s) 340, 500, 644, 899, 998, 1125
BceAI ACGGC 1 cut(s) 768
BcgI CGANNNNNNTGC 2 cut(s) 60, 94
BciT130I CCWGG 6 cut(s) 267, 400, 453, 735, 858, 1182
BclI TGATCA 1 cut(s) 229
BcnI CCSGG 1 cut(s) 414
BcoDI GTCTC 1 cut(s) 618
BfaI CTAG 2 cut(s) 969, 1143
BfmI CTRYAG 1 cut(s) 793
BglI GCCNNNNNGGC 1 cut(s) 789
BglII AGATCT 1 cut(s) 167
BisI GCNGC 5 cut(s) 246, 447, 582, 621, 913
BlpI GCTNAGC 1 cut(s) 899
BlsI GCNGC 5 cut(s) 247, 448, 583, 622, 914
Bme1390I CCNGG 7 cut(s) 267, 400, 414, 453, 735, 858, 1182
Bme18I GGWCC 2 cut(s) 404, 962
BmeT110I CYCGRG 1 cut(s) 715
BmgT120I GGNCC 2 cut(s) 404, 962
BmiI GGNNCC 5 cut(s) 214, 264, 271, 405, 802
BmrFI CCNGG 7 cut(s) 267, 400, 414, 453, 735, 858, 1182
BmrI ACTGGG 1 cut(s) 507
BmsI GCATC 4 cut(s) 232, 295, 479, 862
BmuI ACTGGG 1 cut(s) 507
Bpu10I CCTNAGC 1 cut(s) 111
Bpu1102I GCTNAGC 1 cut(s) 899
BpuEI CTTGAG 1 cut(s) 600
BpuMI CCSGG 1 cut(s) 414
BsaBI GATNNNNATC 1 cut(s) 331
BsaI GGTCTC 1 cut(s) 618
BsaJI CCNNGG 3 cut(s) 273, 399, 452
Bsc4I CCNNNNNNNGG 4 cut(s) 413, 821, 863, 1078
Bse1I ACTGG 1 cut(s) 502
Bse8I GATNNNNATC 1 cut(s) 331
BseBI CCWGG 6 cut(s) 267, 400, 453, 735, 858, 1182
BseDI CCNNGG 3 cut(s) 273, 399, 452
BseGI GGATG 5 cut(s) 395, 511, 760, 891, 1136
BseJI GATNNNNATC 1 cut(s) 331
BseLI CCNNNNNNNGG 4 cut(s) 413, 821, 863, 1078
BseMII CTCAG 3 cut(s) 102, 537, 913
BseNI ACTGG 1 cut(s) 502
BseRI GAGGAG 4 cut(s) 793, 835, 1078, 1171
BseXI GCAGC 5 cut(s) 232, 458, 593, 607, 924
BshNI GGYRCC 2 cut(s) 212, 262
BsiHKAI GWGCWC 3 cut(s) 20, 376, 1000
BsiHKCI CYCGRG 1 cut(s) 715
BsiSI CCGG 1 cut(s) 413
BslFI GGGAC 1 cut(s) 1146
BslI CCNNNNNNNGG 4 cut(s) 413, 821, 863, 1078
BsmAI GTCTC 1 cut(s) 618
BsmFI GGGAC 1 cut(s) 1146
Bso31I GGTCTC 1 cut(s) 618
BsoBI CYCGRG 1 cut(s) 715
Bsp1286I GDGCHC 3 cut(s) 20, 376, 1000
Bsp143I GATC 5 cut(s) 167, 229, 326, 416, 629
Bsp1720I GCTNAGC 1 cut(s) 899
Bsp19I CCATGG 1 cut(s) 273
BspCNI CTCAG 3 cut(s) 103, 536, 912
BspHI TCATGA 1 cut(s) 639
BspLI GGNNCC 5 cut(s) 214, 264, 271, 405, 802
BspPI GGATC 1 cut(s) 424
BspT107I GGYRCC 2 cut(s) 212, 262
BspTNI GGTCTC 1 cut(s) 618
BsrI ACTGG 1 cut(s) 502
BssECI CCNNGG 3 cut(s) 273, 399, 452
BssMI GATC 5 cut(s) 167, 229, 326, 416, 629
BssT1I CCWWGG 1 cut(s) 273
Bst2UI CCWGG 6 cut(s) 267, 400, 453, 735, 858, 1182
Bst4CI ACNGT 6 cut(s) 928, 949, 1021, 1103, 1153, 1178
BstC8I GCNNGC 2 cut(s) 917, 1124
BstDEI CTNAG 3 cut(s) 111, 523, 899
BstDSI CCRYGG 1 cut(s) 273
BstF5I GGATG 5 cut(s) 395, 511, 760, 891, 1136
BstKTI GATC 5 cut(s) 170, 232, 329, 419, 632
BstMAI GTCTC 1 cut(s) 618
BstMBI GATC 5 cut(s) 167, 229, 326, 416, 629
BstMWI GCNNNNNNNGC 8 cut(s) 68, 84, 578, 689, 698, 789, 798, 909
BstNI CCWGG 6 cut(s) 267, 400, 453, 735, 858, 1182
BstNSI RCATGY 2 cut(s) 561, 569
BstSCI CCNGG 7 cut(s) 265, 398, 412, 451, 733, 856, 1180
BstSFI CTRYAG 1 cut(s) 793
BstV1I GCAGC 5 cut(s) 232, 458, 593, 607, 924
BstX2I RGATCY 1 cut(s) 167
BstYI RGATCY 1 cut(s) 167
BtgI CCRYGG 1 cut(s) 273
BtsCI GGATG 5 cut(s) 395, 511, 760, 891, 1136
BtsIMutI CAGTG 1 cut(s) 1017
Cac8I GCNNGC 2 cut(s) 917, 1124
CaiI CAGNNNCTG 3 cut(s) 185, 452, 932
CciI TCATGA 1 cut(s) 639
Cfr13I GGNCC 2 cut(s) 404, 962
Csp6I GTAC 4 cut(s) 43, 177, 213, 1147
CspCI CAANNNNNGTGG 2 cut(s) 990, 1025
CviAII CATG 9 cut(s) 259, 274, 538, 558, 566, 640, 751, 1030, 1049
CviQI GTAC 4 cut(s) 43, 177, 213, 1147
DdeI CTNAG 3 cut(s) 111, 523, 899
DpnI GATC 5 cut(s) 169, 231, 328, 418, 631
DpnII GATC 5 cut(s) 167, 229, 326, 416, 629
Eco130I CCWWGG 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 618
Eco47I GGWCC 2 cut(s) 404, 962
Eco88I CYCGRG 1 cut(s) 715
EcoRI GAATTC 2 cut(s) 527, 686
EcoRII CCWGG 6 cut(s) 265, 398, 451, 733, 856, 1180
EcoT14I CCWWGG 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 9 cut(s) 262, 277, 541, 561, 569, 643, 754, 1033, 1052
FalI AAGNNNNNCTT 2 cut(s) 1052, 1084
FaqI GGGAC 1 cut(s) 1146
FatI CATG 9 cut(s) 258, 273, 537, 557, 565, 639, 750, 1029, 1048
FauNDI CATATG 2 cut(s) 379, 586
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 5 cut(s) 246, 447, 582, 621, 913
FokI GGATG 5 cut(s) 382, 518, 767, 878, 1143
Fsp4HI GCNGC 5 cut(s) 246, 447, 582, 621, 913
FspBI CTAG 2 cut(s) 969, 1143
GluI GCNGC 5 cut(s) 246, 447, 582, 621, 913
HapII CCGG 1 cut(s) 413
Hin1II CATG 9 cut(s) 262, 277, 541, 561, 569, 643, 754, 1033, 1052
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 8 cut(s) 116, 182, 730, 853, 861, 1033, 1090, 1159
HpaI GTTAAC 1 cut(s) 280
HpaII CCGG 1 cut(s) 413
HphI GGTGA 4 cut(s) 321, 521, 593, 1093
Hpy166II GTNNAC 6 cut(s) 280, 563, 673, 712, 1149, 1174
Hpy188I TCNGA 9 cut(s) 82, 322, 421, 526, 941, 995, 1038, 1095, 1130
Hpy188III TCNNGA 3 cut(s) 239, 543, 640
Hpy8I GTNNAC 6 cut(s) 280, 563, 673, 712, 1149, 1174
HpyAV CCTTC 2 cut(s) 782, 1078
HpyCH4III ACNGT 6 cut(s) 928, 949, 1021, 1103, 1153, 1178
HpyCH4IV ACGT 2 cut(s) 38, 370
HpyCH4V TGCA 5 cut(s) 98, 446, 623, 1057, 1108
HpyF10VI GCNNNNNNNGC 8 cut(s) 68, 84, 578, 689, 698, 789, 798, 909
HpyF3I CTNAG 3 cut(s) 111, 523, 899
HpySE526I ACGT 2 cut(s) 38, 370
Hsp92II CATG 9 cut(s) 262, 277, 541, 561, 569, 643, 754, 1033, 1052
KpnI GGTACC 1 cut(s) 216
Ksp22I TGATCA 1 cut(s) 229
KspAI GTTAAC 1 cut(s) 280
Kzo9I GATC 5 cut(s) 167, 229, 326, 416, 629
LmnI GCTCC 4 cut(s) 379, 806, 848, 1065
Lsp1109I GCAGC 5 cut(s) 232, 458, 593, 607, 924
LweI GCATC 4 cut(s) 232, 295, 479, 862
MaeI CTAG 2 cut(s) 969, 1143
MaeII ACGT 2 cut(s) 38, 370
MaeIII GTNAC 4 cut(s) 351, 489, 509, 949
MalI GATC 5 cut(s) 169, 231, 328, 418, 631
MboI GATC 5 cut(s) 167, 229, 326, 416, 629
MboII GAAGA 1 cut(s) 954
MfeI CAATTG 1 cut(s) 288
MflI RGATCY 1 cut(s) 167
MhlI GDGCHC 3 cut(s) 20, 376, 1000
MluCI AATT 7 cut(s) 161, 233, 288, 527, 607, 686, 1186
MlyI GAGTC 4 cut(s) 110, 855, 1042, 1168
MmeI TCCRAC 1 cut(s) 483
MnlI CCTC 7 cut(s) 57, 106, 197, 814, 856, 1056, 1149
MroXI GAANNNNTTC 1 cut(s) 426
MseI TTAA 6 cut(s) 24, 192, 279, 591, 740, 1189
MslI CAYNNNNRTG 1 cut(s) 536
MspI CCGG 1 cut(s) 413
MspR9I CCNGG 7 cut(s) 267, 400, 414, 453, 735, 858, 1182
MunI CAATTG 1 cut(s) 288
MvaI CCWGG 6 cut(s) 267, 400, 453, 735, 858, 1182
MwoI GCNNNNNNNGC 8 cut(s) 68, 84, 578, 689, 698, 789, 798, 909
NciI CCSGG 1 cut(s) 414
NcoI CCATGG 1 cut(s) 273
NdeI CATATG 2 cut(s) 379, 586
NdeII GATC 5 cut(s) 167, 229, 326, 416, 629
NlaIII CATG 9 cut(s) 262, 277, 541, 561, 569, 643, 754, 1033, 1052
NlaIV GGNNCC 5 cut(s) 214, 264, 271, 405, 802
NmeAIII GCCGAG 1 cut(s) 843
NmuCI GTSAC 4 cut(s) 351, 489, 509, 949
NspI RCATGY 2 cut(s) 561, 569
PagI TCATGA 1 cut(s) 639
PciI ACATGT 1 cut(s) 565
PdmI GAANNNNTTC 1 cut(s) 426
PfeI GAWTC 4 cut(s) 182, 730, 853, 1090
PflMI CCANNNNNTGG 2 cut(s) 821, 1078
PfoI TCCNGGA 3 cut(s) 412, 856, 1180
PkrI GCNGC 5 cut(s) 247, 448, 583, 622, 914
PleI GAGTC 4 cut(s) 110, 855, 1041, 1167
PpsI GAGTC 4 cut(s) 110, 855, 1041, 1167
PscI ACATGT 1 cut(s) 565
Psp6I CCWGG 6 cut(s) 265, 398, 451, 733, 856, 1180
PspGI CCWGG 6 cut(s) 265, 398, 451, 733, 856, 1180
PspN4I GGNNCC 5 cut(s) 214, 264, 271, 405, 802
PspPI GGNCC 2 cut(s) 404, 962
PstNI CAGNNNCTG 3 cut(s) 185, 452, 932
PsuI RGATCY 1 cut(s) 167
RsaI GTAC 4 cut(s) 44, 178, 214, 1148
RsaNI GTAC 4 cut(s) 43, 177, 213, 1147
RseI CAYNNNNRTG 1 cut(s) 536
SaqAI TTAA 6 cut(s) 24, 192, 279, 591, 740, 1189
SatI GCNGC 5 cut(s) 246, 447, 582, 621, 913
Sau3AI GATC 5 cut(s) 167, 229, 326, 416, 629
Sau96I GGNCC 2 cut(s) 404, 962
SchI GAGTC 4 cut(s) 110, 855, 1042, 1168
ScrFI CCNGG 7 cut(s) 267, 400, 414, 453, 735, 858, 1182
SduI GDGCHC 3 cut(s) 20, 376, 1000
SfaNI GCATC 4 cut(s) 232, 295, 479, 862
SfcI CTRYAG 1 cut(s) 793
SinI GGWCC 2 cut(s) 404, 962
SmiMI CAYNNNNRTG 1 cut(s) 536
SmlI CTYRAG 1 cut(s) 615
SmoI CTYRAG 1 cut(s) 615
Sse9I AATT 7 cut(s) 161, 233, 288, 527, 607, 686, 1186
SspI AATATT 1 cut(s) 255
SspMI CTAG 2 cut(s) 969, 1143
StyD4I CCNGG 7 cut(s) 265, 398, 412, 451, 733, 856, 1180
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 6 cut(s) 928, 949, 1021, 1103, 1153, 1178
TaiI ACGT 2 cut(s) 41, 373
TaqI TCGA 1 cut(s) 240
TasI AATT 7 cut(s) 161, 233, 288, 527, 607, 686, 1186
TfiI GAWTC 4 cut(s) 182, 730, 853, 1090
Tru1I TTAA 6 cut(s) 24, 192, 279, 591, 740, 1189
Tru9I TTAA 6 cut(s) 24, 192, 279, 591, 740, 1189
TscAI CASTG 1 cut(s) 1024
TseFI GTSAC 4 cut(s) 351, 489, 509, 949
TseI GCWGC 5 cut(s) 245, 446, 581, 620, 912
Tsp45I GTSAC 4 cut(s) 351, 489, 509, 949
TspDTI ATGAA 4 cut(s) 247, 378, 384, 771
TspRI CASTG 1 cut(s) 1024
Van91I CCANNNNNTGG 2 cut(s) 821, 1078
VpaK11BI GGWCC 2 cut(s) 404, 962
XapI RAATTY 2 cut(s) 527, 686
XceI RCATGY 2 cut(s) 561, 569
XmnI GAANNNNTTC 1 cut(s) 426
XspI CTAG 2 cut(s) 969, 1143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.