Rw1G020470

Xylem serine proteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0014944
Physical Location & Seq
Forward (+)
44135493 .. 44140489
4997 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G020470.1

Sequence Viewer

Length: 2244 bp
ATGGGGAGCAAGAGACTCAGCTTCCATTCCCTTATTCTTCTTTTCCATATGATCCTAATAGGCCTTGTTTGTGTCAAGGGAGATGAGCAACACCAAAAGAAGTTTTACATTGTATATTTGGGGGATCACCCTGCTGATCATGAAGACTCTGCAGCCCAAACACATATGAATGTCCTCTCATCTGTAACAGGAAGCTATTCTGGTGCTAGAGAGTCCATGGTTTATAGCTATACAAAGAGCTTCAATGCATTTGCTGCTAAGCTTTCCAGTGACGAAGCTGAAAAGCTATCAAGAATGGATCAAGTTCTCTCTGTGATTCCAAATAGGTATCACAAGCTGCACACAACAAGGTCATGGAACTTCATTGGGTTACCATTAAAAGCTAGAAGAGATTTAAAAAGTGAAAGTGACATTATAGTTGGTCTCTTTGATACAGGAATTACTCCAGAGTCCCAAAGCTTTAAGGATGAAGGCTTTACTCCTCCACCAGCAAAATGGAAAGGAACTTGTGGCCAGTTTGCTAATTTCTCAGGATGCAACAACAAAATCATAGGAGCTAGCTACTTCAAGCTTGATGGCACTCCAGACCCAGCTGACATCTTATCTCCAGTGGATGTGGATGGACATGGGACTCACACATCATCGACCTTAGCTGGAAACCTAGTGCCGAATGCCAACCTCTATGGCCTTGCTAAGGGCACTGCACGAGGGGCAGTGCCATCGGCTAGGGTGTCCATGTACAAAGTATGTTGGGCTAGTTTCGGCTGCTCTGACATGGACATTCTTGCTGCGTATGAAGCTGCCATACACGATGGCGTGGATGTCATATCAATTTCCATTGGTGGAGGGGGAGCCTCTTATACTTTGGATAGTATAGCAGTTGGTTCATTTCATGCCATGAAGAATGGTATTATTACAGTGGCCTCAGCTGGAAATGATGGACCAAGTTTGGGGAGTATTGTCAACCATGCACCATGGTTGGTAACTGTGGCAGCTAGTGGCATTGATAGGCAGTTCATTAGCAAAGTTCATTTGGGCAATGGGAAGAATTTTGCAGGGATTGGAGTGAGCACATTTAATCCAAATAAGACGTTGTACCCTCTTGTTAGTGGGGCTGATGTAGCACAAAGCTCGGAAAGCAAAGAAAATGCAAGGTTCTGTGCTGAGGACTCATTAGACCCTAAAAAAGTAGAGGGAAAGATTGTTTTCTGCCAATTTAGATCTTGGGGTACAGATTCTGTCATTAAAGGAAATGGAGGAGTTGGTACCATTGTTGAAAATGATCAATTTCTCGATGCTGCTCAAATATTCATGGCACCAGGAACCATGGTTAACTCTACAATTGGCGAAAGCATTGATGCCTATATACATTCAGCAAGATCACCATCAGCAGTTATATTCAAGTCACAAGAAGCACAAAAACGTGCTCCATATGTTGCTTCATTTTCATCCAGGGGTCCAAATCCGGGATCAGAACACATTCTCAAGCCTGATATTGCAGCACCTGGGATTGACATTTTGGCATCTTACACTCCATTGAAGTCACTTACTGGGTTGGATGGTGACACACAATACTCAGAATTCACACTCATGTCTGGAACTTCAATGGCATGTCCACATGTTGCTGGTGTAGCAGCATATGTTAAGTCATTTCACCCGAATTGGTCTCAAGCTGCAATAAGATCTGCCATCATGACCACAGCAACACCGATGAGCCATAGAGTGAACACAGAAGCAGAATTCGCCTATGGTGCTGGTCAAGTGAACCCGAGAAAAGCTGTGAATCCAGGCTTAATCTATGACATGGATGAAATGTCATACATTCAGTTCCTATGCCATGAAGGCTATAGTGGCTCCTCATTAGCCCCATTAGTTGGCTCAAAGTCCATAAACTGCTCCTCGTTGATTCCTGGACTCGGCTACGATGCTCTCAACTATCCCACCATCCAGCTCAGCTTGAAAAGTAGCAAGCAACCGACAGTAGCTGTTTTCAGAAGAACTGTCACTAATGTTGGTCCTGCTAGTTCCACATACAATGCCACCATCAGAGCACCAAAGGGAGTGGAAATCACTGTCAAACCCATGAGTCTGACTTTCTCTCATGCTTTGCAAAAGAGGAGCTTCCAAGTTGTGGTGAAGGCGAATCCGATGGACAGTATGCAAATGGTGTCTGGCTCGCTCGGATGGAAAAGCTCTAGGTACACCGTGAGGAGTCCCATTGTGGTTTACAGTCCAGGAAATTAA

Protein Analysis

747

Amino Acids

79.79

Weight (kDa)

8.33

Isoelectric Point (pI)

30.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 36 - 114 2.1e-18 Peptidase inhibitor I9
MORF_dom PF21864 46 - 113 5.7e-06 Multiple organellar RNA editing factor 1, MORF domain
Peptidase_S8 PF00082 136 - 586 3.2e-46 Subtilase family
fn3_6 PF17766 644 - 742 2.1e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00230 AT4G00230
fragaria_vesca FvH4_7g12740
malus_domestica MD02G1196900.v1.1
prunus_persica Prupe.2G151900_v2.0.a1
pyrus_communis pycom02g16020
rosa_chinensis RchiOBHm_Chr1g0351761
rosa_laevigata RLG00000028385
rosa_multiflora Rmu_sc0001933.1_g000013
rosa_roxburghii Rroxscaffold_4G00303570
rosa_rugosa Rorug01G0221200
rosa_samantha Rh1AG235200 Rh1BG205700 Rh1CG219800 Rh1DG232700
rosa_wichuraiana Rw1G020470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1265
AccB1I GGYRCC 2 cut(s) 1265, 1315
AccB7I CCANNNNNTGG 2 cut(s) 1874, 2131
AclWI GGATC 4 cut(s) 46, 132, 306, 1477
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 3 cut(s) 1048, 1580, 1739
AfaI GTAC 5 cut(s) 740, 1097, 1231, 1267, 2201
AfiI CCNNNNNNNGG 6 cut(s) 694, 950, 1466, 1874, 1916, 2131
AflIII ACRYGT 1 cut(s) 1618
AgsI TTSAA 7 cut(s) 244, 568, 1277, 1402, 1540, 1605, 1960
AjnI CCWGG 6 cut(s) 1318, 1451, 1504, 1786, 1909, 2233
Alw21I GWGCWC 3 cut(s) 1073, 1429, 2053
Alw26I GTCTC 3 cut(s) 7, 428, 1671
AlwI GGATC 4 cut(s) 46, 132, 306, 1477
AlwNI CAGNNNCTG 3 cut(s) 1238, 1505, 1985
Ama87I CYCGRG 1 cut(s) 1768
AoxI GGCC 4 cut(s) 61, 511, 685, 921
ApoI RAATTY 3 cut(s) 1048, 1580, 1739
Asp700I GAANNNNTTC 2 cut(s) 196, 1479
Asp718I GGTACC 1 cut(s) 1265
AspS9I GGNCC 3 cut(s) 941, 1457, 2015
AsuC2I CCSGG 1 cut(s) 1467
AsuHPI GGTGA 5 cut(s) 119, 1374, 1574, 1646, 2146
AsuNHI GCTAGC 1 cut(s) 557
AvaI CYCGRG 1 cut(s) 1768
AvaII GGWCC 3 cut(s) 941, 1457, 2015
BaeGI GKGCMC 1 cut(s) 701
BaeI ACNNNNGTAYC 2 cut(s) 1257, 1290
BalI TGGCCA 1 cut(s) 513
BanI GGYRCC 2 cut(s) 1265, 1315
BarI GAAGNNNNNNTAC 2 cut(s) 789, 821
BauI CACGAG 1 cut(s) 705
BbsI GAAGAC 1 cut(s) 150
Bbv12I GWGCWC 3 cut(s) 1073, 1429, 2053
BbvCI CCTCAGC 2 cut(s) 925, 1164
BcgI CGANNNNNNTGC 4 cut(s) 702, 736, 1113, 1147
BciT130I CCWGG 6 cut(s) 1320, 1453, 1506, 1788, 1911, 2235
BclI TGATCA 2 cut(s) 136, 1282
BcnI CCSGG 1 cut(s) 1467
BcoDI GTCTC 3 cut(s) 7, 428, 1671
BfaI CTAG 9 cut(s) 207, 384, 558, 662, 726, 756, 996, 2022, 2196
BfmI CTRYAG 2 cut(s) 150, 1846
BglI GCCNNNNNGGC 1 cut(s) 1842
BglII AGATCT 2 cut(s) 1220, 1682
BlpI GCTNAGC 2 cut(s) 258, 1952
Bme1390I CCNGG 7 cut(s) 1320, 1453, 1467, 1506, 1788, 1911, 2235
Bme18I GGWCC 3 cut(s) 941, 1457, 2015
BmeT110I CYCGRG 1 cut(s) 1768
BmgT120I GGNCC 3 cut(s) 941, 1457, 2015
BmiI GGNNCC 6 cut(s) 853, 1267, 1317, 1324, 1458, 1855
BmrFI CCNGG 7 cut(s) 1320, 1453, 1467, 1506, 1788, 1911, 2235
BmrI ACTGGG 1 cut(s) 1560
BmsI GCATC 5 cut(s) 524, 1285, 1348, 1532, 1915
BmtI GCTAGC 1 cut(s) 561
BmuI ACTGGG 1 cut(s) 1560
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 3 cut(s) 429, 567, 591
Bpu10I CCTNAGC 4 cut(s) 649, 693, 925, 1164
Bpu1102I GCTNAGC 2 cut(s) 258, 1952
BpuEI CTTGAG 2 cut(s) 1469, 1653
BpuMI CCSGG 1 cut(s) 1467
BsaBI GATNNNNATC 1 cut(s) 1384
BsaI GGTCTC 2 cut(s) 428, 1671
BsaJI CCNNGG 5 cut(s) 216, 974, 1326, 1452, 1505
Bsc4I CCNNNNNNNGG 6 cut(s) 694, 950, 1466, 1874, 1916, 2131
Bse1I ACTGG 4 cut(s) 267, 514, 608, 1555
Bse3DI GCAATG 1 cut(s) 1045
Bse8I GATNNNNATC 1 cut(s) 1384
BseBI CCWGG 6 cut(s) 1320, 1453, 1506, 1788, 1911, 2235
BseDI CCNNGG 5 cut(s) 216, 974, 1326, 1452, 1505
BseJI GATNNNNATC 1 cut(s) 1384
BseLI CCNNNNNNNGG 6 cut(s) 694, 950, 1466, 1874, 1916, 2131
BseMI GCAATG 1 cut(s) 1045
BseMII CTCAG 6 cut(s) 31, 543, 939, 1155, 1590, 1966
BseNI ACTGG 4 cut(s) 267, 514, 608, 1555
BseRI GAGGAG 6 cut(s) 471, 1272, 1846, 1888, 2131, 2224
BseSI GKGCMC 1 cut(s) 701
BseYI CCCAGC 1 cut(s) 589
BsgI GTGCAG 2 cut(s) 323, 687
BshFI GGCC 4 cut(s) 63, 513, 687, 923
BshNI GGYRCC 2 cut(s) 1265, 1315
BsiHKAI GWGCWC 3 cut(s) 1073, 1429, 2053
BsiHKCI CYCGRG 1 cut(s) 1768
BsiSI CCGG 1 cut(s) 1466
BslFI GGGAC 3 cut(s) 436, 643, 2199
BslI CCNNNNNNNGG 6 cut(s) 694, 950, 1466, 1874, 1916, 2131
BsmAI GTCTC 3 cut(s) 7, 428, 1671
BsmFI GGGAC 3 cut(s) 436, 643, 2199
BsmI GAATGC 1 cut(s) 676
BsnI GGCC 4 cut(s) 63, 513, 687, 923
Bso31I GGTCTC 2 cut(s) 428, 1671
BsoBI CYCGRG 1 cut(s) 1768
Bsp1286I GDGCHC 4 cut(s) 701, 1073, 1429, 2053
Bsp1407I TGTACA 1 cut(s) 738
Bsp143I GATC 9 cut(s) 51, 124, 136, 298, 1220, 1282, 1379, 1469, 1682
Bsp1720I GCTNAGC 2 cut(s) 258, 1952
Bsp19I CCATGG 3 cut(s) 216, 974, 1326
BspANI GGCC 4 cut(s) 63, 513, 687, 923
BspCNI CTCAG 6 cut(s) 30, 542, 938, 1156, 1589, 1965
BspHI TCATGA 2 cut(s) 139, 1692
BspLI GGNNCC 6 cut(s) 853, 1267, 1317, 1324, 1458, 1855
BspMAI CTGCAG 1 cut(s) 154
BspOI GCTAGC 1 cut(s) 561
BspPI GGATC 4 cut(s) 46, 132, 306, 1477
BspT107I GGYRCC 2 cut(s) 1265, 1315
BspTNI GGTCTC 2 cut(s) 428, 1671
BsrDI GCAATG 1 cut(s) 1045
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 4 cut(s) 267, 514, 608, 1555
BssECI CCNNGG 5 cut(s) 216, 974, 1326, 1452, 1505
BssMI GATC 9 cut(s) 51, 124, 136, 298, 1220, 1282, 1379, 1469, 1682
BssSI CACGAG 1 cut(s) 705
BssT1I CCWWGG 3 cut(s) 216, 974, 1326
Bst2BI CACGAG 1 cut(s) 705
Bst2UI CCWGG 6 cut(s) 1320, 1453, 1506, 1788, 1911, 2235
Bst4CI ACNGT 8 cut(s) 919, 988, 1981, 2002, 2074, 2156, 2206, 2231
Bst6I CTCTTC 1 cut(s) 382
BstAPI GCANNNNNTGC 1 cut(s) 254
BstAUI TGTACA 1 cut(s) 738
BstC8I GCNNGC 3 cut(s) 559, 1970, 2177
BstDEI CTNAG 9 cut(s) 17, 258, 529, 649, 693, 925, 1164, 1576, 1952
BstDSI CCRYGG 3 cut(s) 216, 974, 1326
BstEII GGTNACC 1 cut(s) 369
BstENI CCTNNNNNAGG 1 cut(s) 692
BstKTI GATC 9 cut(s) 54, 127, 139, 301, 1223, 1285, 1382, 1472, 1685
BstMAI GTCTC 3 cut(s) 7, 428, 1671
BstMBI GATC 9 cut(s) 51, 124, 136, 298, 1220, 1282, 1379, 1469, 1682
BstNI CCWGG 6 cut(s) 1320, 1453, 1506, 1788, 1911, 2235
BstNSI RCATGY 2 cut(s) 1614, 1622
BstPI GGTNACC 1 cut(s) 369
BstSCI CCNGG 7 cut(s) 1318, 1451, 1465, 1504, 1786, 1909, 2233
BstSFI CTRYAG 2 cut(s) 150, 1846
BstSLI GKGCMC 1 cut(s) 701
BstV2I GAAGAC 1 cut(s) 150
BstX2I RGATCY 2 cut(s) 1220, 1682
BstXI CCANNNNNNTGG 1 cut(s) 495
BstYI RGATCY 2 cut(s) 1220, 1682
BsuRI GGCC 4 cut(s) 63, 513, 687, 923
BtgI CCRYGG 3 cut(s) 216, 974, 1326
BtsI GCAGTG 2 cut(s) 699, 720
BtsIMutI CAGTG 6 cut(s) 274, 615, 699, 720, 924, 2070
Cac8I GCNNGC 3 cut(s) 559, 1970, 2177
CaiI CAGNNNCTG 3 cut(s) 1238, 1505, 1985
CciI TCATGA 2 cut(s) 139, 1692
Cfr13I GGNCC 3 cut(s) 941, 1457, 2015
Csp6I GTAC 5 cut(s) 739, 1096, 1230, 1266, 2200
CspCI CAANNNNNGTGG 2 cut(s) 2043, 2078
CviQI GTAC 5 cut(s) 739, 1096, 1230, 1266, 2200
DdeI CTNAG 9 cut(s) 17, 258, 529, 649, 693, 925, 1164, 1576, 1952
DpnI GATC 9 cut(s) 53, 126, 138, 300, 1222, 1284, 1381, 1471, 1684
DpnII GATC 9 cut(s) 51, 124, 136, 298, 1220, 1282, 1379, 1469, 1682
DraI TTTAAA 1 cut(s) 396
EaeI YGGCCR 1 cut(s) 511
Eam1104I CTCTTC 1 cut(s) 382
EarI CTCTTC 1 cut(s) 382
Eco130I CCWWGG 3 cut(s) 216, 974, 1326
Eco147I AGGCCT 1 cut(s) 63
Eco31I GGTCTC 2 cut(s) 428, 1671
Eco47I GGWCC 3 cut(s) 941, 1457, 2015
Eco88I CYCGRG 1 cut(s) 1768
Eco91I GGTNACC 1 cut(s) 369
EcoNI CCTNNNNNAGG 1 cut(s) 692
EcoO65I GGTNACC 1 cut(s) 369
EcoRI GAATTC 2 cut(s) 1580, 1739
EcoRII CCWGG 6 cut(s) 1318, 1451, 1504, 1786, 1909, 2233
EcoT14I CCWWGG 3 cut(s) 216, 974, 1326
EcoT22I ATGCAT 1 cut(s) 250
ErhI CCWWGG 3 cut(s) 216, 974, 1326
FalI AAGNNNNNCTT 2 cut(s) 2105, 2137
FaqI GGGAC 3 cut(s) 436, 643, 2199
FauNDI CATATG 4 cut(s) 48, 165, 1432, 1639
FbaI TGATCA 2 cut(s) 136, 1282
FspBI CTAG 9 cut(s) 207, 384, 558, 662, 726, 756, 996, 2022, 2196
GsaI CCCAGC 1 cut(s) 593
GsuI CTGGAG 3 cut(s) 429, 567, 591
HaeIII GGCC 4 cut(s) 63, 513, 687, 923
HapII CCGG 1 cut(s) 1466
HincII GTYRAC 2 cut(s) 964, 1333
HindII GTYRAC 2 cut(s) 964, 1333
HindIII AAGCTT 3 cut(s) 260, 457, 569
HpaI GTTAAC 1 cut(s) 1333
HpaII CCGG 1 cut(s) 1466
HphI GGTGA 5 cut(s) 119, 1374, 1574, 1646, 2146
Hpy166II GTNNAC 7 cut(s) 964, 1333, 1616, 1726, 1765, 2202, 2227
Hpy188I TCNGA 9 cut(s) 772, 1135, 1474, 1579, 1994, 2048, 2091, 2148, 2183
Hpy188III TCNNGA 8 cut(s) 140, 291, 446, 531, 584, 1292, 1596, 1693
Hpy8I GTNNAC 7 cut(s) 964, 1333, 1616, 1726, 1765, 2202, 2227
HpyAV CCTTC 3 cut(s) 464, 1835, 2131
HpyCH4III ACNGT 8 cut(s) 919, 988, 1981, 2002, 2074, 2156, 2206, 2231
HpyCH4IV ACGT 2 cut(s) 1091, 1423
HpyF3I CTNAG 9 cut(s) 17, 258, 529, 649, 693, 925, 1164, 1576, 1952
HpySE526I ACGT 2 cut(s) 1091, 1423
KpnI GGTACC 1 cut(s) 1269
Ksp22I TGATCA 2 cut(s) 136, 1282
KspAI GTTAAC 1 cut(s) 1333
Kzo9I GATC 9 cut(s) 51, 124, 136, 298, 1220, 1282, 1379, 1469, 1682
LmnI GCTCC 7 cut(s) 6, 554, 851, 1432, 1859, 1901, 2118
LweI GCATC 5 cut(s) 524, 1285, 1348, 1532, 1915
MaeI CTAG 9 cut(s) 207, 384, 558, 662, 726, 756, 996, 2022, 2196
MaeII ACGT 2 cut(s) 1091, 1423
MaeIII GTNAC 9 cut(s) 184, 269, 369, 407, 982, 1404, 1542, 1562, 2002
MalI GATC 9 cut(s) 53, 126, 138, 300, 1222, 1284, 1381, 1471, 1684
MboI GATC 9 cut(s) 51, 124, 136, 298, 1220, 1282, 1379, 1469, 1682
MboII GAAGA 6 cut(s) 29, 155, 399, 913, 1057, 2007
MfeI CAATTG 1 cut(s) 1341
MflI RGATCY 2 cut(s) 1220, 1682
MhlI GDGCHC 4 cut(s) 701, 1073, 1429, 2053
MlsI TGGCCA 1 cut(s) 513
MluNI TGGCCA 1 cut(s) 513
MlyI GAGTC 9 cut(s) 9, 140, 221, 458, 625, 1163, 1908, 2095, 2221
MmeI TCCRAC 1 cut(s) 1536
Mox20I TGGCCA 1 cut(s) 513
Mph1103I ATGCAT 1 cut(s) 250
MroXI GAANNNNTTC 2 cut(s) 196, 1479
MscI TGGCCA 1 cut(s) 513
MseI TTAA 9 cut(s) 377, 395, 462, 1077, 1245, 1332, 1644, 1793, 2242
MslI CAYNNNNRTG 2 cut(s) 168, 1589
Msp20I TGGCCA 1 cut(s) 513
MspA1I CMGCKG 2 cut(s) 593, 929
MspI CCGG 1 cut(s) 1466
MspR9I CCNGG 7 cut(s) 1320, 1453, 1467, 1506, 1788, 1911, 2235
MunI CAATTG 1 cut(s) 1341
Mva1269I GAATGC 1 cut(s) 676
MvaI CCWGG 6 cut(s) 1320, 1453, 1506, 1788, 1911, 2235
NciI CCSGG 1 cut(s) 1467
NcoI CCATGG 3 cut(s) 216, 974, 1326
NdeI CATATG 4 cut(s) 48, 165, 1432, 1639
NdeII GATC 9 cut(s) 51, 124, 136, 298, 1220, 1282, 1379, 1469, 1682
NheI GCTAGC 1 cut(s) 557
NlaIV GGNNCC 6 cut(s) 853, 1267, 1317, 1324, 1458, 1855
NmeAIII GCCGAG 1 cut(s) 1896
NmuCI GTSAC 6 cut(s) 269, 407, 1404, 1542, 1562, 2002
NsiI ATGCAT 1 cut(s) 250
NspI RCATGY 2 cut(s) 1614, 1622
PagI TCATGA 2 cut(s) 139, 1692
PceI AGGCCT 1 cut(s) 63
PciI ACATGT 1 cut(s) 1618
PctI GAATGC 1 cut(s) 676
PdmI GAANNNNTTC 2 cut(s) 196, 1479
PfeI GAWTC 5 cut(s) 316, 1235, 1783, 1906, 2143
PflMI CCANNNNNTGG 2 cut(s) 1874, 2131
PfoI TCCNGGA 3 cut(s) 1465, 1909, 2233
PleI GAGTC 9 cut(s) 9, 140, 220, 457, 625, 1163, 1908, 2094, 2220
PpsI GAGTC 9 cut(s) 9, 140, 220, 457, 625, 1163, 1908, 2094, 2220
PscI ACATGT 1 cut(s) 1618
Psp6I CCWGG 6 cut(s) 1318, 1451, 1504, 1786, 1909, 2233
PspEI GGTNACC 1 cut(s) 369
PspFI CCCAGC 1 cut(s) 589
PspGI CCWGG 6 cut(s) 1318, 1451, 1504, 1786, 1909, 2233
PspN4I GGNNCC 6 cut(s) 853, 1267, 1317, 1324, 1458, 1855
PspPI GGNCC 3 cut(s) 941, 1457, 2015
PstI CTGCAG 1 cut(s) 154
PstNI CAGNNNCTG 3 cut(s) 1238, 1505, 1985
PsuI RGATCY 2 cut(s) 1220, 1682
PvuII CAGCTG 2 cut(s) 593, 929
RsaI GTAC 5 cut(s) 740, 1097, 1231, 1267, 2201
RsaNI GTAC 5 cut(s) 739, 1096, 1230, 1266, 2200
RseI CAYNNNNRTG 2 cut(s) 168, 1589
SaqAI TTAA 9 cut(s) 377, 395, 462, 1077, 1245, 1332, 1644, 1793, 2242
Sau3AI GATC 9 cut(s) 51, 124, 136, 298, 1220, 1282, 1379, 1469, 1682
Sau96I GGNCC 3 cut(s) 941, 1457, 2015
SchI GAGTC 9 cut(s) 9, 140, 221, 458, 625, 1163, 1908, 2095, 2221
ScrFI CCNGG 7 cut(s) 1320, 1453, 1467, 1506, 1788, 1911, 2235
SduI GDGCHC 4 cut(s) 701, 1073, 1429, 2053
SfaNI GCATC 5 cut(s) 524, 1285, 1348, 1532, 1915
SfcI CTRYAG 2 cut(s) 150, 1846
SinI GGWCC 3 cut(s) 941, 1457, 2015
SmiMI CAYNNNNRTG 2 cut(s) 168, 1589
SmlI CTYRAG 2 cut(s) 1484, 1668
SmoI CTYRAG 2 cut(s) 1484, 1668
SseBI AGGCCT 1 cut(s) 63
SspI AATATT 1 cut(s) 1308
SspMI CTAG 9 cut(s) 207, 384, 558, 662, 726, 756, 996, 2022, 2196
StuI AGGCCT 1 cut(s) 63
StyD4I CCNGG 7 cut(s) 1318, 1451, 1465, 1504, 1786, 1909, 2233
StyI CCWWGG 3 cut(s) 216, 974, 1326
TaaI ACNGT 8 cut(s) 919, 988, 1981, 2002, 2074, 2156, 2206, 2231
TaiI ACGT 2 cut(s) 1094, 1426
TaqI TCGA 2 cut(s) 644, 1293
TatI WGTACW 1 cut(s) 738
TfiI GAWTC 5 cut(s) 316, 1235, 1783, 1906, 2143
Tru1I TTAA 9 cut(s) 377, 395, 462, 1077, 1245, 1332, 1644, 1793, 2242
Tru9I TTAA 9 cut(s) 377, 395, 462, 1077, 1245, 1332, 1644, 1793, 2242
TscAI CASTG 6 cut(s) 274, 615, 706, 720, 924, 2077
TseFI GTSAC 6 cut(s) 269, 407, 1404, 1542, 1562, 2002
Tsp45I GTSAC 6 cut(s) 269, 407, 1404, 1542, 1562, 2002
TspRI CASTG 6 cut(s) 274, 615, 706, 720, 924, 2077
Van91I CCANNNNNTGG 2 cut(s) 1874, 2131
VpaK11BI GGWCC 3 cut(s) 941, 1457, 2015
XagI CCTNNNNNAGG 1 cut(s) 692
XapI RAATTY 3 cut(s) 1048, 1580, 1739
XceI RCATGY 2 cut(s) 1614, 1622
XcmI CCANNNNNNNNNTGG 1 cut(s) 492
XmnI GAANNNNTTC 2 cut(s) 196, 1479
XspI CTAG 9 cut(s) 207, 384, 558, 662, 726, 756, 996, 2022, 2196
Zsp2I ATGCAT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.