FvH4_7g12740

Peptidase inhibitor I9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014944
Physical Location & Seq
Forward (+)
11555647 .. 11564344
8698 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g12740.t1

Sequence Viewer

Length: 4656 bp
ATGGGGAGCATGAGACTCAGCTTCAATTCCCTTCTTCTAATCCATTTGATCCTAATATGCCTTGTTTGTGTCAAAGGAGATGAGCAAGACCGAAAGAAGTTCTACATTGTATACTTGGGAGATCATCCTGTGAATCATGAACACTCTGCAGCCCAAACACATATGAATGTCCTCTCATCTGTAACCGGAAGCTATTCTGATGCTGCAGAGTCCATGGTTTATAGCTACACCAAGAGCTTCAATGCATTTGCTGCTAAGCTTTCTAGTCACGAAGCCGAAAAGCTATCAAGAATGGATCAAGTTCTCTCTGTGACTCCAAATAGGTATCACAAGCTGCACACCACAAGGTCATGGAACTTCATTGGGTTACCATTAACAGCTAGAAGAAATTTAAAAAGTGAAAGTGATATTGTTGTTGGACTCTTTGATACAGATGTTAAAGAACTTATGTATGCTTCTATGTTGGTGGAAAATCTTGTATCAGGAATTACTCCAGAGTCCCAGAGCTTTAAGGATGAAGGATTTGGTCCTCCACCAGCAAAATGGAAAGGAACTTGTGGCCAGTTTGCTAATTTCTCTGGATGCAACAACAAAATCATAGGAGCTAGATATTTCAAGCTTGATGGCACTCCAGACCCAGCTGACATCTTATCTCCAGTGGACGTGGATGGACATGGAACTCACACCTCATCGACCTTAGCTGGGAACCTAGTCCCGAATGCCAACCTCTATGGCCTCGCTAAGGGCACTGCACGAGGGGCAGTGCCCTCAGCTAGGGTGTCCATGTACAAAGTTTGTTGGGCTAGTTTCGGCTGCTCTGACATGGACATTCTTGCTGCGTATGAAGCTGCCATACATGATGGCGTGGATGTCATATCGATTTCCATTGGTGGAGGGGGACCTAGTTATGGTTCAGATAGTATAGCAGTTGGTGCATTTCATGCCATGAAGAAGGGTATCATTACAGTGGCCTCAGCTGGAAATGATGGACCAAGTTTGGGGAGTATTGTTAACCATGCACCATGGTTGGTAACTGTGGCAGCTAGTGGCATTGATAGGCAGTTCATTAACAAAGTTCATTTGGGCAATGGAAAGGATATATTAGGGGTTGGAGTGAGCACATTCAATCCAAAGAAGAAGTTGTACCCTCTTGTTAGTGGGGTTGATGTAGCACAAAGCTCTGAAACCAAAGAAAATGCAAGGTTCTGTCTTGGAGGCTCATTAGACCCTAAAAGAGTAAAGGGAAAGATTGTTTTCTGCCAATTTGGATCTTGGGGTACAGATTCGGTTATTAAAGGAAATGGAGGAGTTGGTACCATTGTTGAAAATGATCAATTTCTCGATGCTGCTCAAATATTTATGGCACCAGGAACCATGGTTAACTCTACAGTTGGAGAAAGCATTGATGCCTATATACATTCAGCAAAATCACCATCAGCAGTTATATTCAAGTCACAAGAAGCACAAAGACGCGCTCCGTATGTTGCTTCATTTTCATCCCGGGGTCCAAATCCGGGATCAGAACACCTTCTCAAGCCTGACATTGCAGCACCTGGGATTAACATTTTGGCATCTTACACTCTATTGAAGACGCTTACTGGGTTGGATGGTGACACACAATACTCAGAATTCACACTAATGTCTGGAACTTCAATGGCATGTCCACATGTTGCTGGTGTAGCAGCATATGTCAAGTCATTTCACCCGAATTGGTCTCAAGCTGCAATAAGATCTGCTATCCTGACCACAGCAACACCGATGAGCCAAAGAGTGAACGCAGAAGCAGAATTCGCCTATGGTGTTGGTCAAGTGAACCCGAGAAAAGCTGTGAATCCAGGCTTAATTTATGACATGGATGAAATGTCATACATTCAGTTCCTATGCCATGAAGGCTATAGTGGTTCCTCATTAGCTCCATTAGTTGGCTCAAAGTCCATAAACTGCTCCTCATTGATTCCTGGACTCGGTTACGATGCTATCAACTATCCCACTATGCAGCTCAGCTTGAAAAGCAGCAAGCAACCAACAGTAGCTGTTTTCAGAAGAACTGTCACTAATGTTGGTCCTGCTAGTTCCACATACAATGCCACTATCAGAGCACCAAAGGGAGTGGAAATCACTGTCAAACCCATGAGCCTGACTTTCTCTCATGCTTTGCAAAAGAGGAGCTTCCAAGTTGAGGTGAAGGCGAATCCGATGGACAGTATGACAATGGTGTCTGGCTCACTTGGATGGAAAAGCTCTAGGTACACTGTGAGGAGTCCCATTGTGGTCTTTAAGCTGGTAGTTTTCATCCCAAATATACTATTTCATTCTCTTCCTTGTGGAGGTAGCATGATGATGATTAGAGCATTCTTGTTGTTCTTGGCTTTCATGGCTGCAACTAGTACTGCTCTGATGGAAACACAGACCTACATTGTTCACATGGACAAGGCGAAAATCCCTGCTGCTGATCACAAAGACCAACAGTACTACTACTACTACAAAGCAGTGTTGGATTCCATTGCCGAACTTTCATCTCAAGAAGAAGAGGAAGAAGTAAGAGGCAAAGCATCAACAACTCCTCAGCTTCTTTACACCTACAACACTGCTATTTCGGGTTTCGCAGCAAGACTCTCAGCCAATCAGCTCAAAGCCTTGAGCCAAATCCATGGTTTCTTATATGCCACACCCGATGAGCTACTAACTCTCCACACCACACACTCTCCTCAGTTTCTTGGCTTACAACAAGGCAAAGGACTTTGGAGTGCTCCCAGCTTGGCCTCGGATGTGATCATTGGTGTTGTTGACACTGGAGTCTGGCCAGAACATGTTAGCTTCAAGGACAAAGGAATGCCCCCCGTACCCTCTCACTGGAAAGGCACTTGTGAAAAAGGCACCAAGTTCTCACAGTCAAATTGTAACAATAAGCTTATTGGTGCAAGATCCTTTTTGCAAGGTTATGAGGCTATTGTTGGAACAGTCAATGAAACAGTGGATTATAGATCCCCTCGTGACTCCGAAGGACATGGAACTCACACTGCTTCAACTGCAGGCGGTAATGTTGTCAAACAAGCAAGCTTGTTTGGCTTAGCAAAGGGCTCGGCAAGTGGAATGAGATATTCTTCAAGAATTGCAGCCTATAAGGCCTGTTGGCCATTGGGGTGTTCTAACTCTGATGTAATGGCTGCTATTGACAGTGCTGTTTCTGATGGTGTAGACATTTTGTCACTCTCATTGGGAGGTCTTTCGAGACCTTATTTTCGTGACAGTATTGCTATAGCTTCATTTGGTGCTGTACAACATGGAGTTTCCGTGTCATTTTCTGCTGGAAATTCAGGTCCATTTCGCTCAAGCGTTAGCAATACCGCGCCTTGGATCCTGACTGTTGCTGCTAGCTACACTGACCGAAGCTTCCCAGTGCTAGTGAAGCTTGGGAATGGACAAACTTTTGAGGGGTCATCATTATATTCAGGTAAGAAAACTAAGCAGCTGCCACTTGTGTATAACAGAACAGCAGGTGGCCAAGGAGCTGAGCATTGTGTCGATGGCTCACTTGACATAAACCTTGTGAAGGGAAAGATTGTGGCATGTGAGCGAGGAACACACAGCCGGACTGAAATTGGAGAGGAAGTGAAGAAAGCAGGAGGAGCTGGAATGCTTCTACTCAATAATGAAGCTGAAGGGGAGGAACTTCTTGCTGATGCTCACATTTTACCAGCCACTTCTCTTGGAGCTATAGCTTCTAAGGCTGTGAGAGAATATGCAGGTGCAGCAAAGAAACCTACAGCTATGCTTGTGTTCCAAGGCACGGTATATGGTAGAACTGCTCCGGTGATGGCAGCATTTTCATCTAGAGGACCAAGCTCAGTTGGACCGGACGTGATCAAGCCTGATGTGACTGCTCCTGGTGTCAACATATTGGCTGCTTGGCCAGCCAAGACTAGCCCAACCAGGCTTAAAAGTGACAAGAGAAAAGTGGTGTTTAACATGGTCTCAGGCACTTCAATGTCTTGCCCTCATGTTAGTGGCTTAGCTGCTCTTATCAAATCAGTGCACAAAGATTGGTCACCTGCAGCAATCAAATCTGCACTCATGACTACAGCTTATACACTAAACAACAAAGGCTCTCCAATTTCTGATTTTGGCTCAAACAATTCTCAACCAGCTAACCCATTTGCGTTTGGTTCGGGGCATGCAGACCCTGAAAGTGCTGCGGATCCGGGACTAATTTACGATATCACCACCAACGACTACCTGCTCTATTTGTGTAGCTTGAATTACACATCTCCCCAAATAGCTCTCTTCTCCAGTGGGATTAGTAACTTCACTTGTCCTAGCAATAGCACAGTTCTTCAGCCCGGCAACTTGAACTACCCTTCACTCTCAGTGGTTTTCAGAAGAGATGGCAGGAAAATGAGTGCGACATACACGAGGACAGTGACAAATGTTGGTGCTATTAACCCTAGTACTTATGCAGTGCAAGTAGAAGCACCCATTGGAGTATCAGTTACTGTTGAGCCTAGGAAATTGGTTTTTAAGAAGATGGGTGAGAAATTGAGCTACAAGGTAAGTTTTGTTGGAATGAGTGCCACAACCAATTCATCCTTTGGATCCTTGGTTTGGGTGAGTGAGAAATATAGGGTTAGAAGTCCCATTGCAGTGATATGGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

1552

Amino Acids

165.48

Weight (kDa)

8.91

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 35 - 113 5.3e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 602 7.4e-45 Subtilase family
fn3_6 PF17766 660 - 758 3.6e-27 Fibronectin type-III domain
Inhibitor_I9 PF05922 804 - 898 2.1e-12 Peptidase inhibitor I9
Peptidase_S8 PF00082 922 - 1390 1.6e-49 Subtilase family
PA PF02225 1157 - 1239 1.4e-09 PA domain
fn3_6 PF17766 1449 - 1548 7.2e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00230 AT4G00230
fragaria_vesca FvH4_7g12740
malus_domestica MD02G1196900.v1.1
prunus_persica Prupe.2G151900_v2.0.a1
pyrus_communis pycom02g16020
rosa_chinensis RchiOBHm_Chr1g0351761
rosa_laevigata RLG00000028385
rosa_multiflora Rmu_sc0001933.1_g000013
rosa_roxburghii Rroxscaffold_4G00303570
rosa_rugosa Rorug01G0221200
rosa_samantha Rh1AG235200 Rh1BG205700 Rh1CG219800 Rh1DG232700
rosa_wichuraiana Rw1G020470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 3488, 3737, 4060
AasI GACNNNNNNGTC 2 cut(s) 2215, 2795
Acc36I ACCTGC 4 cut(s) 3488, 3737, 4060, 4245
Acc65I GGTACC 1 cut(s) 1313
AccB1I GGYRCC 3 cut(s) 1313, 1363, 2876
AccB7I CCANNNNNTGG 1 cut(s) 1922
AccI GTMKAC 2 cut(s) 111, 3198
AccII CGCG 2 cut(s) 1473, 3350
AciI CCGC 3 cut(s) 3036, 3348, 4196
AcoI YGGCCR 5 cut(s) 559, 2801, 3134, 3502, 3911
AcsI RAATTY 4 cut(s) 388, 1628, 1787, 3313
AcuI CTGAAG 2 cut(s) 3681, 4319
AflIII ACRYGT 2 cut(s) 1666, 2809
AhdI GACNNNNNGTC 1 cut(s) 3205
AhlI ACTAGT 1 cut(s) 2384
AjiI CACGTC 2 cut(s) 664, 3862
AjnI CCWGG 6 cut(s) 1366, 1552, 1834, 1957, 3886, 3932
AjuI GAANNNNNNNTTGG 2 cut(s) 4461, 4493
Alw21I GWGCWC 4 cut(s) 1121, 2101, 2752, 4038
Alw26I GTCTC 4 cut(s) 7, 1719, 3226, 3979
Alw44I GTGCAC 1 cut(s) 4034
AlwNI CAGNNNCTG 4 cut(s) 1553, 2033, 4184, 4493
Ama87I CYCGRG 2 cut(s) 1500, 1816
AoxI GGCC 9 cut(s) 559, 733, 969, 2760, 2801, 3126, 3134, 3502, 3911
ApaLI GTGCAC 1 cut(s) 4034
ApoI RAATTY 4 cut(s) 388, 1628, 1787, 3313
ArsI GACNNNNNNTTYG 2 cut(s) 3225, 3257
Asp700I GAANNNNTTC 2 cut(s) 193, 1527
Asp718I GGTACC 1 cut(s) 1313
AspA2I CCTAGG 1 cut(s) 4502
AspLEI GCGC 2 cut(s) 1475, 3352
AspS9I GGNCC 8 cut(s) 527, 899, 989, 1505, 2063, 3320, 3839, 3854
AsuC2I CCSGG 5 cut(s) 1501, 1502, 1515, 4203, 4341
AsuHPI GGTGA 9 cut(s) 1422, 1622, 1694, 2194, 3826, 4041, 4213, 4541, 4618
AsuNHI GCTAGC 1 cut(s) 3374
AvaI CYCGRG 2 cut(s) 1500, 1816
AvaII GGWCC 8 cut(s) 527, 899, 989, 1505, 2063, 3320, 3839, 3854
AvrII CCTAGG 1 cut(s) 4502
BaeGI GKGCMC 3 cut(s) 749, 768, 4038
BaeI ACNNNNGTAYC 4 cut(s) 420, 453, 1305, 1338
BalI TGGCCA 5 cut(s) 561, 2803, 3136, 3504, 3913
BamHI GGATCC 3 cut(s) 3357, 4198, 4592
BanI GGYRCC 3 cut(s) 1313, 1363, 2876
BanII GRGCYC 1 cut(s) 3083
BarI GAAGNNNNNNTAC 4 cut(s) 837, 869, 1127, 1159
BauI CACGAG 3 cut(s) 753, 2991, 4411
BbsI GAAGAC 1 cut(s) 1595
Bbv12I GWGCWC 4 cut(s) 1121, 2101, 2752, 4038
BbvCI CCTCAGC 3 cut(s) 769, 973, 2565
BcgI CGANNNNNNTGC 2 cut(s) 3063, 3097
BciT130I CCWGG 6 cut(s) 1368, 1554, 1836, 1959, 3888, 3934
BclI TGATCA 4 cut(s) 1330, 2452, 2772, 3864
BcnI CCSGG 5 cut(s) 1501, 1502, 1515, 4203, 4341
BcoDI GTCTC 4 cut(s) 7, 1719, 3226, 3979
BcuI ACTAGT 1 cut(s) 2384
BfuAI ACCTGC 4 cut(s) 3488, 3737, 4060, 4245
BglI GCCNNNNNGGC 2 cut(s) 1890, 3125
BglII AGATCT 1 cut(s) 1730
BlnI CCTAGG 1 cut(s) 4502
BlpI GCTNAGC 5 cut(s) 255, 2000, 3070, 3513, 4012
BmcAI AGTACT 3 cut(s) 2389, 2471, 4450
Bme18I GGWCC 8 cut(s) 527, 899, 989, 1505, 2063, 3320, 3839, 3854
BmeRI GACNNNNNGTC 1 cut(s) 3205
BmeT110I CYCGRG 2 cut(s) 1500, 1816
BmgBI CACGTC 2 cut(s) 664, 3862
BmgT120I GGNCC 8 cut(s) 527, 899, 989, 1505, 2063, 3320, 3839, 3854
BmrI ACTGGG 2 cut(s) 1608, 3392
BmsI GCATC 8 cut(s) 190, 572, 1333, 1396, 1580, 1963, 2561, 3673
BmtI GCTAGC 1 cut(s) 3378
BmuI ACTGGG 2 cut(s) 1608, 3392
BpiI GAAGAC 1 cut(s) 1595
BpmI CTGGAG 5 cut(s) 477, 615, 639, 2814, 4273
Bpu10I CCTNAGC 5 cut(s) 697, 741, 769, 973, 2565
Bpu1102I GCTNAGC 5 cut(s) 255, 2000, 3070, 3513, 4012
BpuEI CTTGAG 5 cut(s) 1517, 1701, 2505, 2659, 3316
BpuMI CCSGG 5 cut(s) 1501, 1502, 1515, 4203, 4341
Bsa29I ATCGAT 1 cut(s) 878
BsaI GGTCTC 3 cut(s) 1719, 3226, 3979
BsaWI WCCGGW 3 cut(s) 185, 3811, 3856
Bse1I ACTGG 7 cut(s) 562, 656, 1603, 2797, 2858, 3398, 4290
Bse3DI GCAATG 4 cut(s) 1093, 1542, 2502, 4635
BseBI CCWGG 6 cut(s) 1368, 1554, 1836, 1959, 3888, 3934
BseCI ATCGAT 1 cut(s) 878
BseMI GCAATG 4 cut(s) 1093, 1542, 2502, 4635
BseNI ACTGG 7 cut(s) 562, 656, 1603, 2797, 2858, 3398, 4290
BseRI GAGGAG 7 cut(s) 1320, 1936, 2179, 2272, 2553, 2697, 3642
BseSI GKGCMC 3 cut(s) 749, 768, 4038
BseYI CCCAGC 3 cut(s) 637, 701, 2753
BsgI GTGCAG 4 cut(s) 320, 735, 3771, 4053
Bsh1236I CGCG 2 cut(s) 1473, 3350
BshFI GGCC 9 cut(s) 561, 735, 971, 2762, 2803, 3128, 3136, 3504, 3913
BshNI GGYRCC 3 cut(s) 1313, 1363, 2876
BshVI ATCGAT 1 cut(s) 878
BsiHKAI GWGCWC 4 cut(s) 1121, 2101, 2752, 4038
BsiHKCI CYCGRG 2 cut(s) 1500, 1816
BsiSI CCGG 8 cut(s) 186, 1501, 1514, 3592, 3812, 3857, 4202, 4341
BslFI GGGAC 6 cut(s) 484, 698, 912, 2247, 4218, 4617
BsmAI GTCTC 4 cut(s) 7, 1719, 3226, 3979
BsmFI GGGAC 6 cut(s) 484, 698, 912, 2247, 4218, 4617
BsmI GAATGC 4 cut(s) 724, 2351, 2838, 3642
BsnI GGCC 9 cut(s) 561, 735, 971, 2762, 2803, 3128, 3136, 3504, 3913
Bso31I GGTCTC 3 cut(s) 1719, 3226, 3979
BsoBI CYCGRG 2 cut(s) 1500, 1816
Bsp1286I GDGCHC 7 cut(s) 749, 768, 1121, 2101, 2752, 3083, 4038
Bsp1407I TGTACA 2 cut(s) 786, 3277
Bsp1720I GCTNAGC 5 cut(s) 255, 2000, 3070, 3513, 4012
Bsp19I CCATGG 4 cut(s) 213, 1022, 1374, 2650
BspACI CCGC 3 cut(s) 3036, 3348, 4196
BspANI GGCC 9 cut(s) 561, 735, 971, 2762, 2803, 3128, 3136, 3504, 3913
BspDI ATCGAT 1 cut(s) 878
BspFNI CGCG 2 cut(s) 1473, 3350
BspHI TCATGA 2 cut(s) 136, 4074
BspMAI CTGCAG 4 cut(s) 151, 208, 3034, 4057
BspMI ACCTGC 4 cut(s) 3488, 3737, 4060, 4245
BspOI GCTAGC 1 cut(s) 3378
BspT107I GGYRCC 3 cut(s) 1313, 1363, 2876
BspTNI GGTCTC 3 cut(s) 1719, 3226, 3979
BsrDI GCAATG 4 cut(s) 1093, 1542, 2502, 4635
BsrGI TGTACA 2 cut(s) 786, 3277
BsrI ACTGG 7 cut(s) 562, 656, 1603, 2797, 2858, 3398, 4290
BssNAI GTATAC 1 cut(s) 112
BssSI CACGAG 3 cut(s) 753, 2991, 4411
BssT1I CCWWGG 9 cut(s) 213, 1022, 1374, 2650, 3353, 3505, 3784, 4502, 4596
Bst1107I GTATAC 1 cut(s) 112
Bst2BI CACGAG 3 cut(s) 753, 2991, 4411
Bst2UI CCWGG 6 cut(s) 1368, 1554, 1836, 1959, 3888, 3934
Bst6I CTCTTC 4 cut(s) 2322, 2523, 4289, 4375
BstAPI GCANNNNNTGC 3 cut(s) 251, 932, 941
BstAUI TGTACA 2 cut(s) 786, 3277
BstC8I GCNNGC 6 cut(s) 2018, 3034, 3058, 3376, 3915, 4176
BstDSI CCRYGG 4 cut(s) 213, 1022, 1374, 2650
BstEII GGTNACC 2 cut(s) 366, 4047
BstENI CCTNNNNNAGG 4 cut(s) 740, 772, 2325, 3551
BstFNI CGCG 2 cut(s) 1473, 3350
BstHHI GCGC 2 cut(s) 1475, 3352
BstMAI GTCTC 4 cut(s) 7, 1719, 3226, 3979
BstNI CCWGG 6 cut(s) 1368, 1554, 1836, 1959, 3888, 3934
BstNSI RCATGY 5 cut(s) 1662, 1670, 2813, 3573, 4178
BstPI GGTNACC 2 cut(s) 366, 4047
BstSLI GKGCMC 3 cut(s) 749, 768, 4038
BstUI CGCG 2 cut(s) 1473, 3350
BstV2I GAAGAC 1 cut(s) 1595
BstX2I RGATCY 7 cut(s) 1268, 1730, 2924, 2984, 3357, 4198, 4592
BstXI CCANNNNNNTGG 2 cut(s) 543, 2651
BstYI RGATCY 7 cut(s) 1268, 1730, 2924, 2984, 3357, 4198, 4592
BstZ17I GTATAC 1 cut(s) 112
Bsu15I ATCGAT 1 cut(s) 878
BsuRI GGCC 9 cut(s) 561, 735, 971, 2762, 2803, 3128, 3136, 3504, 3913
BsuTUI ATCGAT 1 cut(s) 878
BtgI CCRYGG 4 cut(s) 213, 1022, 1374, 2650
BtrI CACGTC 2 cut(s) 664, 3862
BtsI GCAGTG 7 cut(s) 747, 768, 2496, 2586, 3018, 4464, 4647
BveI ACCTGC 4 cut(s) 3488, 3737, 4060, 4245
Cac8I GCNNGC 6 cut(s) 2018, 3034, 3058, 3376, 3915, 4176
CaiI CAGNNNCTG 4 cut(s) 1553, 2033, 4184, 4493
CciI TCATGA 2 cut(s) 136, 4074
CfoI GCGC 2 cut(s) 1475, 3352
Cfr13I GGNCC 8 cut(s) 527, 899, 989, 1505, 2063, 3320, 3839, 3854
Cfr9I CCCGGG 1 cut(s) 1500
ClaI ATCGAT 1 cut(s) 878
CseI GACGC 2 cut(s) 1479, 1600
CspCI CAANNNNNGTGG 4 cut(s) 2091, 2126, 3691, 3726
DraI TTTAAA 1 cut(s) 393
DrdI GACNNNNNNGTC 2 cut(s) 2215, 2795
DriI GACNNNNNGTC 1 cut(s) 3205
DseDI GACNNNNNNGTC 2 cut(s) 2215, 2795
EaeI YGGCCR 5 cut(s) 559, 2801, 3134, 3502, 3911
Eam1104I CTCTTC 4 cut(s) 2322, 2523, 4289, 4375
Eam1105I GACNNNNNGTC 1 cut(s) 3205
EarI CTCTTC 4 cut(s) 2322, 2523, 4289, 4375
Eco130I CCWWGG 9 cut(s) 213, 1022, 1374, 2650, 3353, 3505, 3784, 4502, 4596
Eco147I AGGCCT 1 cut(s) 3128
Eco24I GRGCYC 1 cut(s) 3083
Eco31I GGTCTC 3 cut(s) 1719, 3226, 3979
Eco32I GATATC 1 cut(s) 4219
Eco47I GGWCC 8 cut(s) 527, 899, 989, 1505, 2063, 3320, 3839, 3854
Eco57I CTGAAG 2 cut(s) 3681, 4319
Eco88I CYCGRG 2 cut(s) 1500, 1816
Eco91I GGTNACC 2 cut(s) 366, 4047
EcoNI CCTNNNNNAGG 4 cut(s) 740, 772, 2325, 3551
EcoO109I RGGNCCY 1 cut(s) 899
EcoO65I GGTNACC 2 cut(s) 366, 4047
EcoRI GAATTC 2 cut(s) 1628, 1787
EcoRII CCWGG 6 cut(s) 1366, 1552, 1834, 1957, 3886, 3932
EcoRV GATATC 1 cut(s) 4219
EcoT14I CCWWGG 9 cut(s) 213, 1022, 1374, 2650, 3353, 3505, 3784, 4502, 4596
EcoT22I ATGCAT 1 cut(s) 247
EcoT38I GRGCYC 1 cut(s) 3083
ErhI CCWWGG 9 cut(s) 213, 1022, 1374, 2650, 3353, 3505, 3784, 4502, 4596
FalI AAGNNNNNCTT 2 cut(s) 2153, 2185
FaqI GGGAC 6 cut(s) 484, 698, 912, 2247, 4218, 4617
FauNDI CATATG 2 cut(s) 162, 1687
FbaI TGATCA 4 cut(s) 1330, 2452, 2772, 3864
FblI GTMKAC 2 cut(s) 111, 3198
FriOI GRGCYC 1 cut(s) 3083
GlaI GCGC 2 cut(s) 1474, 3351
GsaI CCCAGC 3 cut(s) 641, 705, 2757
GsuI CTGGAG 5 cut(s) 477, 615, 639, 2814, 4273
HaeIII GGCC 9 cut(s) 561, 735, 971, 2762, 2803, 3128, 3136, 3504, 3913
HapII CCGG 8 cut(s) 186, 1501, 1514, 3592, 3812, 3857, 4202, 4341
HgaI GACGC 2 cut(s) 1479, 1600
HhaI GCGC 2 cut(s) 1475, 3352
Hin6I GCGC 2 cut(s) 1473, 3350
HinP1I GCGC 2 cut(s) 1473, 3350
HincII GTYRAC 4 cut(s) 1012, 1381, 2788, 3895
HindII GTYRAC 4 cut(s) 1012, 1381, 2788, 3895
HindIII AAGCTT 6 cut(s) 257, 617, 2909, 3058, 3391, 3410
HpaI GTTAAC 2 cut(s) 1012, 1381
HpaII CCGG 8 cut(s) 186, 1501, 1514, 3592, 3812, 3857, 4202, 4341
HphI GGTGA 9 cut(s) 1422, 1622, 1694, 2194, 3826, 4041, 4213, 4541, 4618
HpyCH4IV ACGT 2 cut(s) 663, 3861
HpySE526I ACGT 2 cut(s) 663, 3861
HspAI GCGC 2 cut(s) 1473, 3350
KpnI GGTACC 1 cut(s) 1317
Ksp22I TGATCA 4 cut(s) 1330, 2452, 2772, 3864
KspAI GTTAAC 2 cut(s) 1012, 1381
LweI GCATC 8 cut(s) 190, 572, 1333, 1396, 1580, 1963, 2561, 3673
MaeII ACGT 2 cut(s) 663, 3861
MflI RGATCY 7 cut(s) 1268, 1730, 2924, 2984, 3357, 4198, 4592
MhlI GDGCHC 7 cut(s) 749, 768, 1121, 2101, 2752, 3083, 4038
MlsI TGGCCA 5 cut(s) 561, 2803, 3136, 3504, 3913
MluNI TGGCCA 5 cut(s) 561, 2803, 3136, 3504, 3913
MmeI TCCRAC 8 cut(s) 397, 1090, 1372, 1584, 2475, 2935, 3832, 4540
Mox20I TGGCCA 5 cut(s) 561, 2803, 3136, 3504, 3913
Mph1103I ATGCAT 1 cut(s) 247
MroXI GAANNNNTTC 2 cut(s) 193, 1527
MscI TGGCCA 5 cut(s) 561, 2803, 3136, 3504, 3913
MslI CAYNNNNRTG 9 cut(s) 165, 965, 1637, 2230, 2339, 3142, 3986, 4005, 4640
Msp20I TGGCCA 5 cut(s) 561, 2803, 3136, 3504, 3913
MspA1I CMGCKG 3 cut(s) 641, 977, 3472
MspI CCGG 8 cut(s) 186, 1501, 1514, 3592, 3812, 3857, 4202, 4341
Mva1269I GAATGC 4 cut(s) 724, 2351, 2838, 3642
MvaI CCWGG 6 cut(s) 1368, 1554, 1836, 1959, 3888, 3934
MvnI CGCG 2 cut(s) 1473, 3350
NciI CCSGG 5 cut(s) 1501, 1502, 1515, 4203, 4341
NcoI CCATGG 4 cut(s) 213, 1022, 1374, 2650
NdeI CATATG 2 cut(s) 162, 1687
NheI GCTAGC 1 cut(s) 3374
NmeAIII GCCGAG 1 cut(s) 3062
NsiI ATGCAT 1 cut(s) 247
NspI RCATGY 5 cut(s) 1662, 1670, 2813, 3573, 4178
PaeI GCATGC 1 cut(s) 4178
PagI TCATGA 2 cut(s) 136, 4074
PaqCI CACCTGC 3 cut(s) 3488, 3737, 4060
PceI AGGCCT 1 cut(s) 3128
PciI ACATGT 2 cut(s) 1666, 2809
PctI GAATGC 4 cut(s) 724, 2351, 2838, 3642
PdmI GAANNNNTTC 2 cut(s) 193, 1527
PfeI GAWTC 6 cut(s) 133, 1283, 1831, 1954, 2191, 2498
PflMI CCANNNNNTGG 1 cut(s) 1922
PfoI TCCNGGA 3 cut(s) 1513, 1957, 4201
PpuMI RGGWCCY 1 cut(s) 899
PscI ACATGT 2 cut(s) 1666, 2809
Psp5II RGGWCCY 1 cut(s) 899
Psp6I CCWGG 6 cut(s) 1366, 1552, 1834, 1957, 3886, 3932
PspEI GGTNACC 2 cut(s) 366, 4047
PspFI CCCAGC 3 cut(s) 637, 701, 2753
PspGI CCWGG 6 cut(s) 1366, 1552, 1834, 1957, 3886, 3932
PspPI GGNCC 8 cut(s) 527, 899, 989, 1505, 2063, 3320, 3839, 3854
PspPPI RGGWCCY 1 cut(s) 899
PstI CTGCAG 4 cut(s) 151, 208, 3034, 4057
PstNI CAGNNNCTG 4 cut(s) 1553, 2033, 4184, 4493
PsuI RGATCY 7 cut(s) 1268, 1730, 2924, 2984, 3357, 4198, 4592
PvuII CAGCTG 3 cut(s) 641, 977, 3472
RseI CAYNNNNRTG 9 cut(s) 165, 965, 1637, 2230, 2339, 3142, 3986, 4005, 4640
Sau96I GGNCC 8 cut(s) 527, 899, 989, 1505, 2063, 3320, 3839, 3854
ScaI AGTACT 3 cut(s) 2389, 2471, 4450
SduI GDGCHC 7 cut(s) 749, 768, 1121, 2101, 2752, 3083, 4038
SfaNI GCATC 8 cut(s) 190, 572, 1333, 1396, 1580, 1963, 2561, 3673
SinI GGWCC 8 cut(s) 527, 899, 989, 1505, 2063, 3320, 3839, 3854
SmaI CCCGGG 1 cut(s) 1502
SmiMI CAYNNNNRTG 9 cut(s) 165, 965, 1637, 2230, 2339, 3142, 3986, 4005, 4640
SmlI CTYRAG 5 cut(s) 1532, 1716, 2520, 2638, 3331
SmoI CTYRAG 5 cut(s) 1532, 1716, 2520, 2638, 3331
SpeI ACTAGT 1 cut(s) 2384
SphI GCATGC 1 cut(s) 4178
SseBI AGGCCT 1 cut(s) 3128
SsiI CCGC 3 cut(s) 3036, 3348, 4196
SspI AATATT 1 cut(s) 1356
StuI AGGCCT 1 cut(s) 3128
StyI CCWWGG 9 cut(s) 213, 1022, 1374, 2650, 3353, 3505, 3784, 4502, 4596
TaiI ACGT 2 cut(s) 666, 3864
TaqI TCGA 5 cut(s) 692, 878, 1341, 3230, 3525
TaqII GACCGA 2 cut(s) 105, 3402
TatI WGTACW 5 cut(s) 786, 2387, 2469, 3277, 4448
TfiI GAWTC 6 cut(s) 133, 1283, 1831, 1954, 2191, 2498
TspGWI ACGGA 2 cut(s) 1467, 3283
TspMI CCCGGG 1 cut(s) 1500
Van91I CCANNNNNTGG 1 cut(s) 1922
VneI GTGCAC 1 cut(s) 4034
VpaK11BI GGWCC 8 cut(s) 527, 899, 989, 1505, 2063, 3320, 3839, 3854
XagI CCTNNNNNAGG 4 cut(s) 740, 772, 2325, 3551
XapI RAATTY 4 cut(s) 388, 1628, 1787, 3313
XbaI TCTAGA 1 cut(s) 3833
XceI RCATGY 5 cut(s) 1662, 1670, 2813, 3573, 4178
XcmI CCANNNNNNNNNTGG 1 cut(s) 540
XmaI CCCGGG 1 cut(s) 1500
XmaJI CCTAGG 1 cut(s) 4502
XmiI GTMKAC 2 cut(s) 111, 3198
XmnI GAANNNNTTC 2 cut(s) 193, 1527
ZrmI AGTACT 3 cut(s) 2389, 2471, 4450
Zsp2I ATGCAT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.