Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0014944

Physical Location & Seq

Genomic Coordinates Reverse (-)

18933920 .. 18938830

Gene Structure 4911 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1196900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2310 bp

ATGTATAAAAACAAGAGGAACCAATTTGTGAGGCAGCTGAGTGCAAAGCCAACATTAAATCTTGCAAGCATGTGTAGCAAGAAAATTAGTTCTTCCTTTCTTCTTCTTTTTCTTCTTTATTTGATCTTAGTAGGCGTTGTTTGTGTAAAAGGAGATGACAAAAAGAAGTTTTACATTGTGTACTTGGGAGCTCATCCTGTGAATAATGAGGACTCTGCAGCTTCCCATACGCATATGAAAATCCTCTCATCTGTTAAAGGAAGCTATGCTGATGCTCAAGAGTCCATGGTTTATAGCTATAAAAAGAGCTTCGATGCCTTTGCTGCCAAGCTTTCTAGTGATGAAGCCAAAATATTAGAAAGAATGGACGAGGTTCTCTCCGTGATTCCTAATAGGTATCACAAGCTACACACAACAAGATCATGGGATTTCATTGGGTTACCACAAAAAGCTAGACGAAATTTGAAGACTGAAAGTGACATAATTGTTGGTCTCCTTGATACAGGAATTACTCCAGAGTCCAAAAGCTTTAAGGGAGATGGATTAGGTCCTCCACCAGCAAAGTGGAAAGGAACTTGTGGCCACTTTGCCAATTTTTCTGGATGCAACAACAAGATCATAGGTGCCAATTACTTCAAGCTTGATGGCAATCCAGACCCAGTTGACATCTTATCTCCCTTAGATGTGGACGGCCATGGGACTCATACTTCATCAACCTTAGCAGGCAACCAGGTCCCCAATGCCAACCTCTTCGGCCTAGCCAATGGCACTGCGCGAGGTGCAGTGCCCTCGGCTAGGGTGGCCATGTACAAAGTATGCTGGGCTAGCAGTGGCTGTTCAGACATGGACATCCTAGCAGCGTATGATGCTGCCATACATGACGGCGTCGATGTCATATCAATTTCTATTGGTGGGGGGAGTTCAAGTTATGTGTCGGACATTATAGCAATAGGTGCATTTCATGCCATTAAGAAGGGTATTATTACTGTGGCCTCAGCTGGAAATGATGGACCAAATTTGGGAAGTGTTCTTAATCATGCACCATGGTTGGTAACTGTGGCTGCCAGTGGGATTGATAGGGATTTCATAAGCAAAGTTCATCTGGGCAACGGAAAGGATGTCTCAGGGATTGGAGTGAGCATGTTCAGTCCAAAGAAAAACTTATACCCTATTGTTAGTGGGGTTGATGTAGCACAAAGCTCTCAAACCAAGGAAGGTGCAAGGTTCTGTGTTCAGGATACATTAGACCCTGGAAAGGTAAAGGGCAAGCTTGTTTTCTGCAAACTCGGATCTTGGGGCGCAGATTCTGTGGTTAAGGGAATTGGAGGGGCTGGCATTATAATTGAAGATGATCAGTTTCTTGATGCTGCTCAAATATTCATGGCTCCAGGAACCATGGTCAATTCTACAATCGGAGATATCATAGATGAGTACATTCACACAACAAGATCACCATCAGCAGTGATATACAAGTCTCAACAAACAATGAAACGTGCTCCGTTTGTTGCTTCATTCTCCTCCCGGGGTCCAAATCAAGGATCCAATCGCATCCTCAAGCCTGATATTGCAGCACCTGGGGTTGACATCTTGGCGTCTTACACTCTTAGGAAGTCGCTCACTGGATTGGCAGGTGACACACAATTTTCAGAATTTACACTCATGTCTGGAACTTCCATGGCATGCCCGCACGTTGCTGGAGTAGCAGCATACGTAAAGTCATTCCACCCGAATTGGTCACCGGCTGCAATTAGATCTGCCATCTTGACCACTGCAAAACCAATGAGCCAGCGGGTGAACGGCGATGCAGAATTCGCCTATGGTGCCGGTCAAGTGAACCCGGCTAAAGCTGTGAACCCGGGCCTAGTCTATGACATGGATGAAATATTGTCCTACATTCAGTTCCTCTGCCATGAAGGCTACAAAGGGTCCTCATTAGCCCCTTTGGTGGGCTCAAAAACCCTAAACTGCTCCTCATTGCTTCCTGGTCAAGGCTATGATGCTCTCAACTATCCCACCATGCAGCTCAGCTTGAAAAGCAACAAGCAACCAACAGTTGGTGTTTTCAGAAGAACTGTCACCAATGTGGGCCCTGCAAAATCCATCTACAATGCCACTATTAGAGCACCAAAGGGAGTTGAAATCACTGTGAAGCCCATGAGTCTATCTTTCTCCACCACCTTGGAAAGGAAGACCTTCCAAGTTGTGGTGAAGGCGAACCCTATGACCAGTATGCGGATGGTATCCGGCTCACTCGGATGGAAAAGCACTCGATACACCGTGAGGAGCCCCATTGTGATTTACAGTCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005618 GO:0005623 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009505 GO:0016787 GO:0017171 GO:0019538 GO:0030312 GO:0043170 GO:0044238 GO:0044464 GO:0070011 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

770

Amino Acids

82.19

Weight (kDa)

9.28

Isoelectric Point (pI)

28.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	58 - 137	2.1e-16	Peptidase inhibitor I9
Peptidase_S8	PF00082	159 - 609	8.5e-50	Subtilase family
fn3_6	PF17766	668 - 766	3.9e-26	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014944)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00230 AT4G00230
fragaria_vesca	FvH4_7g12740
malus_domestica	MD02G1196900.v1.1
prunus_persica	Prupe.2G151900_v2.0.a1
pyrus_communis	pycom02g16020
rosa_chinensis	RchiOBHm_Chr1g0351761
rosa_laevigata	RLG00000028385
rosa_multiflora	Rmu_sc0001933.1_g000013
rosa_roxburghii	Rroxscaffold_4G00303570
rosa_rugosa	Rorug01G0221200
rosa_samantha	Rh1AG235200 Rh1BG205700 Rh1CG219800 Rh1DG232700
rosa_wichuraiana	Rw1G020470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1340
AarI	CACCTGC	1 cut(s)	1619
Acc36I	ACCTGC	1 cut(s)	1619
AccB1I	GGYRCC	2 cut(s)	623, 1820
AccB7I	CCANNNNNTGG	2 cut(s)	2054, 2203
AccII	CGCG	1 cut(s)	775
AciI	CCGC	3 cut(s)	1685, 1789, 2233
AclWI	GGATC	3 cut(s)	1297, 1533, 1546
AcoI	YGGCCR	3 cut(s)	580, 691, 801
AcsI	RAATTY	4 cut(s)	460, 1015, 1649, 1808
AcyI	GRCGYC	2 cut(s)	885, 1592
AfaI	GTAC	3 cut(s)	182, 809, 1433
AgsI	TTSAA	6 cut(s)	466, 637, 924, 1346, 2032, 2138
AjnI	CCWGG	5 cut(s)	729, 1249, 1387, 1573, 1981
AleI	CACNNNNGTG	1 cut(s)	2081
Alw21I	GWGCWC	3 cut(s)	193, 1498, 2125
Alw26I	GTCTC	3 cut(s)	497, 1126, 1479
AlwI	GGATC	3 cut(s)	1297, 1533, 1546
AlwNI	CAGNNNCTG	3 cut(s)	834, 1307, 1574
Ama87I	CYCGRG	2 cut(s)	1521, 1855
AoxI	GGCC	7 cut(s)	580, 691, 754, 801, 990, 1858, 2086
ApaI	GGGCCC	1 cut(s)	2090
ApoI	RAATTY	4 cut(s)	460, 1015, 1649, 1808
Asp700I	GAANNNNTTC	1 cut(s)	2192
AspLEI	GCGC	2 cut(s)	775, 1301
AspS9I	GGNCC	8 cut(s)	548, 733, 1010, 1526, 1858, 1926, 2086, 2087
AsuC2I	CCSGG	5 cut(s)	1522, 1523, 1838, 1856, 1857
AsuHPI	GGTGA	6 cut(s)	1443, 1643, 1728, 1804, 2068, 2218
AsuNHI	GCTAGC	1 cut(s)	824
AvaI	CYCGRG	2 cut(s)	1521, 1855
AvaII	GGWCC	5 cut(s)	548, 733, 1010, 1526, 1926
BaeGI	GKGCMC	2 cut(s)	789, 2090
BalI	TGGCCA	2 cut(s)	582, 803
BamHI	GGATCC	1 cut(s)	1538
BanI	GGYRCC	2 cut(s)	623, 1820
BanII	GRGCYC	4 cut(s)	193, 1952, 2090, 2288
BbsI	GAAGAC	2 cut(s)	473, 2195
Bbv12I	GWGCWC	3 cut(s)	193, 1498, 2125
BbvCI	CCTCAGC	1 cut(s)	994
BccI	CCATC	8 cut(s)	533, 638, 1001, 1462, 1766, 2108, 2230, 2250
BceAI	ACGGC	3 cut(s)	706, 898, 1813
BcgI	CGANNNNNNTGC	4 cut(s)	302, 336, 771, 805
BciT130I	CCWGG	5 cut(s)	731, 1251, 1389, 1575, 1983
BciVI	GTATCC	2 cut(s)	1231, 2251
BclI	TGATCA	1 cut(s)	1351
BcnI	CCSGG	5 cut(s)	1522, 1523, 1838, 1856, 1857
BcoDI	GTCTC	3 cut(s)	497, 1126, 1479
BfaI	CTAG	7 cut(s)	336, 453, 758, 795, 825, 854, 1862
BfmI	CTRYAG	1 cut(s)	216
BfuAI	ACCTGC	1 cut(s)	1619
BfuI	GTATCC	2 cut(s)	1231, 2251
BglI	GCCNNNNNGGC	1 cut(s)	1914
BglII	AGATCT	1 cut(s)	1751
BlpI	GCTNAGC	1 cut(s)	2024
Bme18I	GGWCC	5 cut(s)	548, 733, 1010, 1526, 1926
BmeT110I	CYCGRG	2 cut(s)	1521, 1855
BmgT120I	GGNCC	8 cut(s)	548, 733, 1010, 1526, 1858, 1926, 2086, 2087
BmrI	ACTGGG	1 cut(s)	653
BmsI	GCATC	8 cut(s)	262, 304, 593, 856, 1354, 1557, 1792, 1987
BmtI	GCTAGC	1 cut(s)	828
BmuI	ACTGGG	1 cut(s)	653
BpiI	GAAGAC	2 cut(s)	473, 2195
BplI	GAGNNNNNCTC	2 cut(s)	362, 394
BpmI	CTGGAG	3 cut(s)	498, 1371, 1716
Bpu10I	CCTNAGC	2 cut(s)	718, 994
Bpu1102I	GCTNAGC	1 cut(s)	2024
BpuEI	CTTGAG	2 cut(s)	261, 1538
BpuMI	CCSGG	5 cut(s)	1522, 1523, 1838, 1856, 1857
BsaAI	YACGTR	1 cut(s)	1711
BsaBI	GATNNNNATC	2 cut(s)	648, 1453
BsaHI	GRCGYC	2 cut(s)	885, 1592
BsaI	GGTCTC	1 cut(s)	497
BsaXI	ACNNNNNCTCC	2 cut(s)	910, 940
Bse118I	RCCGGY	2 cut(s)	1738, 1823
Bse1I	ACTGG	4 cut(s)	659, 1065, 1624, 2226
Bse3DI	GCAATG	1 cut(s)	1973
Bse8I	GATNNNNATC	2 cut(s)	648, 1453
BseBI	CCWGG	5 cut(s)	731, 1251, 1389, 1575, 1983
BseGI	GGATG	8 cut(s)	193, 608, 849, 1123, 1548, 1882, 2241, 2261
BseJI	GATNNNNATC	2 cut(s)	648, 1453
BseMI	GCAATG	1 cut(s)	1973
BseMII	CTCAG	4 cut(s)	29, 1008, 1137, 2038
BseNI	ACTGG	4 cut(s)	659, 1065, 1624, 2226
BseRI	GAGGAG	3 cut(s)	1507, 1960, 2296
BseSI	GKGCMC	2 cut(s)	789, 2090
BseYI	CCCAGC	1 cut(s)	819
BsgI	GTGCAG	1 cut(s)	801
Bsh1236I	CGCG	1 cut(s)	775
BshFI	GGCC	7 cut(s)	582, 693, 756, 803, 992, 1860, 2088
BshNI	GGYRCC	2 cut(s)	623, 1820
BsiHKAI	GWGCWC	3 cut(s)	193, 1498, 2125
BsiHKCI	CYCGRG	2 cut(s)	1521, 1855
BsiSI	CCGG	6 cut(s)	1522, 1739, 1824, 1838, 1856, 2244
BslFI	GGGAC	2 cut(s)	712, 719
BsmAI	GTCTC	3 cut(s)	497, 1126, 1479
BsmFI	GGGAC	2 cut(s)	712, 719
BsnI	GGCC	7 cut(s)	582, 693, 756, 803, 992, 1860, 2088
Bso31I	GGTCTC	1 cut(s)	497
BsoBI	CYCGRG	2 cut(s)	1521, 1855
Bsp120I	GGGCCC	1 cut(s)	2086
Bsp1286I	GDGCHC	7 cut(s)	193, 789, 1498, 1952, 2090, 2125, 2288
Bsp1407I	TGTACA	1 cut(s)	807
Bsp143I	GATC	8 cut(s)	123, 419, 615, 1289, 1351, 1448, 1538, 1751
Bsp1720I	GCTNAGC	1 cut(s)	2024
Bsp19I	CCATGG	5 cut(s)	285, 694, 1043, 1395, 1674
BspACI	CCGC	3 cut(s)	1685, 1789, 2233
BspANI	GGCC	7 cut(s)	582, 693, 756, 803, 992, 1860, 2088
BspCNI	CTCAG	4 cut(s)	30, 1007, 1136, 2037
BspFNI	CGCG	1 cut(s)	775
BspMAI	CTGCAG	1 cut(s)	220
BspMI	ACCTGC	1 cut(s)	1619
BspOI	GCTAGC	1 cut(s)	828
BspPI	GGATC	3 cut(s)	1297, 1533, 1546
BspT107I	GGYRCC	2 cut(s)	623, 1820
BspTNI	GGTCTC	1 cut(s)	497
BsrDI	GCAATG	1 cut(s)	1973
BsrFI	RCCGGY	2 cut(s)	1738, 1823
BsrGI	TGTACA	1 cut(s)	807
BsrI	ACTGG	4 cut(s)	659, 1065, 1624, 2226
BssAI	RCCGGY	2 cut(s)	1738, 1823
BssMI	GATC	8 cut(s)	123, 419, 615, 1289, 1351, 1448, 1538, 1751
BssNI	GRCGYC	2 cut(s)	885, 1592
BssT1I	CCWWGG	7 cut(s)	285, 694, 1043, 1209, 1395, 1674, 2178
Bst2UI	CCWGG	5 cut(s)	731, 1251, 1389, 1575, 1983
Bst4CI	ACNGT	7 cut(s)	988, 1057, 2053, 2074, 2146, 2278, 2303
Bst6I	CTCTTC	1 cut(s)	755
BstACI	GRCGYC	2 cut(s)	885, 1592
BstAPI	GCANNNNNTGC	2 cut(s)	953, 962
BstAUI	TGTACA	1 cut(s)	807
BstBAI	YACGTR	1 cut(s)	1711
BstC8I	GCNNGC	8 cut(s)	67, 724, 826, 1268, 1333, 1681, 1685, 1787
BstDEI	CTNAG	8 cut(s)	38, 127, 679, 718, 994, 1123, 1604, 2024
BstDSI	CCRYGG	5 cut(s)	285, 694, 1043, 1395, 1674
BstEII	GGTNACC	2 cut(s)	438, 1734
BstENI	CCTNNNNNAGG	3 cut(s)	793, 1986, 2182
BstF5I	GGATG	8 cut(s)	193, 608, 849, 1123, 1548, 1882, 2241, 2261
BstFNI	CGCG	1 cut(s)	775
BstHHI	GCGC	2 cut(s)	775, 1301
BstKTI	GATC	8 cut(s)	126, 422, 618, 1292, 1354, 1451, 1541, 1754
BstMAI	GTCTC	3 cut(s)	497, 1126, 1479
BstMBI	GATC	8 cut(s)	123, 419, 615, 1289, 1351, 1448, 1538, 1751
BstNI	CCWGG	5 cut(s)	731, 1251, 1389, 1575, 1983
BstNSI	RCATGY	3 cut(s)	73, 1144, 1683
BstPI	GGTNACC	2 cut(s)	438, 1734
BstSFI	CTRYAG	1 cut(s)	216
BstSLI	GKGCMC	2 cut(s)	789, 2090
BstSNI	TACGTA	1 cut(s)	1711
BstUI	CGCG	1 cut(s)	775
BstV2I	GAAGAC	2 cut(s)	473, 2195
BstX2I	RGATCY	3 cut(s)	1289, 1538, 1751
BstXI	CCANNNNNNTGG	2 cut(s)	564, 2179
BstYI	RGATCY	3 cut(s)	1289, 1538, 1751
BsuI	GTATCC	2 cut(s)	1231, 2251
BsuRI	GGCC	7 cut(s)	582, 693, 756, 803, 992, 1860, 2088
BtgI	CCRYGG	5 cut(s)	285, 694, 1043, 1395, 1674
BtgZI	GCGATG	1 cut(s)	1815
BtsCI	GGATG	8 cut(s)	193, 608, 849, 1123, 1548, 1882, 2241, 2261
BtsI	GCAGTG	5 cut(s)	768, 789, 835, 1467, 1767
BtsIMutI	CAGTG	8 cut(s)	768, 789, 835, 1072, 1467, 1617, 1767, 2142
BveI	ACCTGC	1 cut(s)	1619
Cac8I	GCNNGC	8 cut(s)	67, 724, 826, 1268, 1333, 1681, 1685, 1787
CaiI	CAGNNNCTG	3 cut(s)	834, 1307, 1574
CfoI	GCGC	2 cut(s)	775, 1301
Cfr10I	RCCGGY	2 cut(s)	1738, 1823
Cfr13I	GGNCC	8 cut(s)	548, 733, 1010, 1526, 1858, 1926, 2086, 2087
Cfr9I	CCCGGG	2 cut(s)	1521, 1855
CseI	GACGC	2 cut(s)	874, 1581
CsiI	ACCWGGT	1 cut(s)	729
Csp6I	GTAC	3 cut(s)	181, 808, 1432
CspCI	CAANNNNNGTGG	2 cut(s)	1712, 1747
CviQI	GTAC	3 cut(s)	181, 808, 1432
DdeI	CTNAG	8 cut(s)	38, 127, 679, 718, 994, 1123, 1604, 2024
DpnI	GATC	8 cut(s)	125, 421, 617, 1291, 1353, 1450, 1540, 1753
DpnII	GATC	8 cut(s)	123, 419, 615, 1289, 1351, 1448, 1538, 1751
EaeI	YGGCCR	3 cut(s)	580, 691, 801
Eam1104I	CTCTTC	1 cut(s)	755
EarI	CTCTTC	1 cut(s)	755
Ecl136II	GAGCTC	1 cut(s)	191
Eco105I	TACGTA	1 cut(s)	1711
Eco130I	CCWWGG	7 cut(s)	285, 694, 1043, 1209, 1395, 1674, 2178
Eco24I	GRGCYC	4 cut(s)	193, 1952, 2090, 2288
Eco31I	GGTCTC	1 cut(s)	497
Eco32I	GATATC	1 cut(s)	1420
Eco47I	GGWCC	5 cut(s)	548, 733, 1010, 1526, 1926
Eco53kI	GAGCTC	1 cut(s)	191
Eco88I	CYCGRG	2 cut(s)	1521, 1855
Eco91I	GGTNACC	2 cut(s)	438, 1734
EcoICRI	GAGCTC	1 cut(s)	191
EcoNI	CCTNNNNNAGG	3 cut(s)	793, 1986, 2182
EcoO109I	RGGNCCY	4 cut(s)	548, 733, 1926, 2087
EcoO65I	GGTNACC	2 cut(s)	438, 1734
EcoRI	GAATTC	1 cut(s)	1808
EcoRII	CCWGG	5 cut(s)	729, 1249, 1387, 1573, 1981
EcoRV	GATATC	1 cut(s)	1420
EcoT14I	CCWWGG	7 cut(s)	285, 694, 1043, 1209, 1395, 1674, 2178
EcoT38I	GRGCYC	4 cut(s)	193, 1952, 2090, 2288
ErhI	CCWWGG	7 cut(s)	285, 694, 1043, 1209, 1395, 1674, 2178
FalI	AAGNNNNNCTT	2 cut(s)	1145, 1177
FaqI	GGGAC	2 cut(s)	712, 719
FauI	CCCGC	2 cut(s)	1692, 1782
FauNDI	CATATG	1 cut(s)	234
FbaI	TGATCA	1 cut(s)	1351
FokI	GGATG	8 cut(s)	180, 615, 836, 1130, 1535, 1889, 2248, 2268
FriOI	GRGCYC	4 cut(s)	193, 1952, 2090, 2288
FspBI	CTAG	7 cut(s)	336, 453, 758, 795, 825, 854, 1862
GlaI	GCGC	2 cut(s)	774, 1300
GsaI	CCCAGC	1 cut(s)	823
GsuI	CTGGAG	3 cut(s)	498, 1371, 1716
HaeIII	GGCC	7 cut(s)	582, 693, 756, 803, 992, 1860, 2088
HapII	CCGG	6 cut(s)	1522, 1739, 1824, 1838, 1856, 2244
HgaI	GACGC	2 cut(s)	874, 1581
HhaI	GCGC	2 cut(s)	775, 1301
Hin1I	GRCGYC	2 cut(s)	885, 1592
Hin6I	GCGC	2 cut(s)	773, 1299
HinP1I	GCGC	2 cut(s)	773, 1299
HincII	GTYRAC	2 cut(s)	664, 1582
HindII	GTYRAC	2 cut(s)	664, 1582
HindIII	AAGCTT	4 cut(s)	329, 526, 638, 1268
HinfI	GANTC	7 cut(s)	212, 281, 385, 518, 700, 1304, 2158
HpaII	CCGG	6 cut(s)	1522, 1739, 1824, 1838, 1856, 2244
HphI	GGTGA	6 cut(s)	1443, 1643, 1728, 1804, 2068, 2218
Hpy166II	GTNNAC	7 cut(s)	181, 664, 688, 1582, 1795, 1834, 1852
Hpy188I	TCNGA	7 cut(s)	841, 937, 1289, 1415, 1648, 2066, 2255
Hpy188III	TCNNGA	8 cut(s)	278, 515, 600, 653, 1235, 1361, 1665, 1762
Hpy8I	GTNNAC	7 cut(s)	181, 664, 688, 1582, 1795, 1834, 1852
Hpy99I	CGWCG	1 cut(s)	890
HpyAV	CCTTC	5 cut(s)	967, 1208, 1907, 2203, 2203
HpyCH4III	ACNGT	7 cut(s)	988, 1057, 2053, 2074, 2146, 2278, 2303
HpyCH4IV	ACGT	3 cut(s)	1492, 1689, 1710
HpyF3I	CTNAG	8 cut(s)	38, 127, 679, 718, 994, 1123, 1604, 2024
HpySE526I	ACGT	3 cut(s)	1492, 1689, 1710
Hsp92I	GRCGYC	2 cut(s)	885, 1592
HspAI	GCGC	2 cut(s)	773, 1299
Ksp22I	TGATCA	1 cut(s)	1351
Kzo9I	GATC	8 cut(s)	123, 419, 615, 1289, 1351, 1448, 1538, 1751
LmnI	GCTCC	5 cut(s)	188, 1390, 1501, 1973, 2283
LweI	GCATC	8 cut(s)	262, 304, 593, 856, 1354, 1557, 1792, 1987
MabI	ACCWGGT	1 cut(s)	729
MaeI	CTAG	7 cut(s)	336, 453, 758, 795, 825, 854, 1862
MaeII	ACGT	3 cut(s)	1492, 1689, 1710
MaeIII	GTNAC	6 cut(s)	438, 476, 1051, 1631, 1734, 2074
MalI	GATC	8 cut(s)	125, 421, 617, 1291, 1353, 1450, 1540, 1753
MboI	GATC	8 cut(s)	123, 419, 615, 1289, 1351, 1448, 1538, 1751
MboII	GAAGA	9 cut(s)	84, 92, 95, 104, 478, 742, 1358, 2079, 2200
MflI	RGATCY	3 cut(s)	1289, 1538, 1751
MhlI	GDGCHC	7 cut(s)	193, 789, 1498, 1952, 2090, 2125, 2288
MlsI	TGGCCA	2 cut(s)	582, 803
MluNI	TGGCCA	2 cut(s)	582, 803
MlyI	GAGTC	5 cut(s)	206, 290, 527, 694, 2167
MmeI	TCCRAC	1 cut(s)	915
Mox20I	TGGCCA	2 cut(s)	582, 803
MroXI	GAANNNNTTC	1 cut(s)	2192
MscI	TGGCCA	2 cut(s)	582, 803
MseI	TTAA	6 cut(s)	56, 255, 531, 969, 1032, 1314
MslI	CAYNNNNRTG	2 cut(s)	2081, 2254
Msp20I	TGGCCA	2 cut(s)	582, 803
MspA1I	CMGCKG	3 cut(s)	37, 998, 1789
MspI	CCGG	6 cut(s)	1522, 1739, 1824, 1838, 1856, 2244
MvaI	CCWGG	5 cut(s)	731, 1251, 1389, 1575, 1983
MvnI	CGCG	1 cut(s)	775
NciI	CCSGG	5 cut(s)	1522, 1523, 1838, 1856, 1857
NcoI	CCATGG	5 cut(s)	285, 694, 1043, 1395, 1674
NdeI	CATATG	1 cut(s)	234
NdeII	GATC	8 cut(s)	123, 419, 615, 1289, 1351, 1448, 1538, 1751
NheI	GCTAGC	1 cut(s)	824
NmeAIII	GCCGAG	1 cut(s)	770
NmuCI	GTSAC	4 cut(s)	476, 1631, 1734, 2074
NspI	RCATGY	3 cut(s)	73, 1144, 1683
OliI	CACNNNNGTG	1 cut(s)	2081
PaeI	GCATGC	1 cut(s)	1683
PaqCI	CACCTGC	1 cut(s)	1619
PdmI	GAANNNNTTC	1 cut(s)	2192
PfeI	GAWTC	2 cut(s)	385, 1304
PflFI	GACNNNGTC	1 cut(s)	884
PflMI	CCANNNNNTGG	2 cut(s)	2054, 2203
PfoI	TCCNGGA	1 cut(s)	1387
PleI	GAGTC	5 cut(s)	206, 289, 526, 694, 2166
PpsI	GAGTC	5 cut(s)	206, 289, 526, 694, 2166
Ppu21I	YACGTR	1 cut(s)	1711
PpuMI	RGGWCCY	3 cut(s)	548, 733, 1926
PsiI	TTATAA	1 cut(s)	1340
Psp124BI	GAGCTC	1 cut(s)	193
Psp5II	RGGWCCY	3 cut(s)	548, 733, 1926
Psp6I	CCWGG	5 cut(s)	729, 1249, 1387, 1573, 1981
PspEI	GGTNACC	2 cut(s)	438, 1734
PspFI	CCCAGC	1 cut(s)	819
PspGI	CCWGG	5 cut(s)	729, 1249, 1387, 1573, 1981
PspOMI	GGGCCC	1 cut(s)	2086
PspPI	GGNCC	8 cut(s)	548, 733, 1010, 1526, 1858, 1926, 2086, 2087
PspPPI	RGGWCCY	3 cut(s)	548, 733, 1926
PstI	CTGCAG	1 cut(s)	220
PstNI	CAGNNNCTG	3 cut(s)	834, 1307, 1574
PsuI	RGATCY	3 cut(s)	1289, 1538, 1751
PsyI	GACNNNGTC	1 cut(s)	884
PvuII	CAGCTG	2 cut(s)	37, 998
RsaI	GTAC	3 cut(s)	182, 809, 1433
RsaNI	GTAC	3 cut(s)	181, 808, 1432
RseI	CAYNNNNRTG	2 cut(s)	2081, 2254
SacI	GAGCTC	1 cut(s)	193
SaqAI	TTAA	6 cut(s)	56, 255, 531, 969, 1032, 1314
Sau3AI	GATC	8 cut(s)	123, 419, 615, 1289, 1351, 1448, 1538, 1751
Sau96I	GGNCC	8 cut(s)	548, 733, 1010, 1526, 1858, 1926, 2086, 2087
SchI	GAGTC	5 cut(s)	206, 290, 527, 694, 2167
SduI	GDGCHC	7 cut(s)	193, 789, 1498, 1952, 2090, 2125, 2288
SexAI	ACCWGGT	1 cut(s)	729
SfaNI	GCATC	8 cut(s)	262, 304, 593, 856, 1354, 1557, 1792, 1987
SfcI	CTRYAG	1 cut(s)	216
SinI	GGWCC	5 cut(s)	548, 733, 1010, 1526, 1926
SmaI	CCCGGG	2 cut(s)	1523, 1857
SmiMI	CAYNNNNRTG	2 cut(s)	2081, 2254
SmlI	CTYRAG	2 cut(s)	276, 1553
SmoI	CTYRAG	2 cut(s)	276, 1553
SnaBI	TACGTA	1 cut(s)	1711
SphI	GCATGC	1 cut(s)	1683
SsiI	CCGC	3 cut(s)	1685, 1789, 2233
SspI	AATATT	3 cut(s)	354, 1377, 1884
SspMI	CTAG	7 cut(s)	336, 453, 758, 795, 825, 854, 1862
SstI	GAGCTC	1 cut(s)	193
StyI	CCWWGG	7 cut(s)	285, 694, 1043, 1209, 1395, 1674, 2178
TaaI	ACNGT	7 cut(s)	988, 1057, 2053, 2074, 2146, 2278, 2303
TaiI	ACGT	3 cut(s)	1495, 1692, 1713
TaqI	TCGA	3 cut(s)	312, 888, 2269
TatI	WGTACW	3 cut(s)	180, 807, 1431
TfiI	GAWTC	2 cut(s)	385, 1304
Tru1I	TTAA	6 cut(s)	56, 255, 531, 969, 1032, 1314
Tru9I	TTAA	6 cut(s)	56, 255, 531, 969, 1032, 1314
TscAI	CASTG	8 cut(s)	775, 789, 835, 1072, 1467, 1624, 1774, 2149
TseFI	GTSAC	4 cut(s)	476, 1631, 1734, 2074
Tsp45I	GTSAC	4 cut(s)	476, 1631, 1734, 2074
TspGWI	ACGGA	3 cut(s)	370, 1125, 1488
TspMI	CCCGGG	2 cut(s)	1521, 1855
TspRI	CASTG	8 cut(s)	775, 789, 835, 1072, 1467, 1624, 1774, 2149
Tth111I	GACNNNGTC	1 cut(s)	884
Van91I	CCANNNNNTGG	2 cut(s)	2054, 2203
VpaK11BI	GGWCC	5 cut(s)	548, 733, 1010, 1526, 1926
XagI	CCTNNNNNAGG	3 cut(s)	793, 1986, 2182
XapI	RAATTY	4 cut(s)	460, 1015, 1649, 1808
XceI	RCATGY	3 cut(s)	73, 1144, 1683
XcmI	CCANNNNNNNNNTGG	2 cut(s)	561, 2233
XmaI	CCCGGG	2 cut(s)	1521, 1855
XmnI	GAANNNNTTC	1 cut(s)	2192
XspI	CTAG	7 cut(s)	336, 453, 758, 795, 825, 854, 1862

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.