RLG00000028385

Peptidase inhibitor I9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0014944
Physical Location & Seq
Reverse (-)
22056850 .. 22067521
10672 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028385

Sequence Viewer

Length: 4746 bp
ATGGGGAGCAAGAGACTCAGCTTCCATTCCCTTATTCTTCTTTTCCATATGATCCTAATAGGCCTTGTTTGTGTCAAGGGAGATGAGCAACACCAAAAGAAGTTTTACATTGTATATTTGGGGGATCACCCTGCTGATCATGAAGACTCTGCAGCCCAAACACATATGAATGTCCTCTCATCTGTAACAGGAAGCTATTCTGGTGCTACAGAGTCCATGGTTTATAGCTATACCAAGAGCTTCAATGCATTTGCTGCTAAGCTGTCCAGTGACGAAGCGGAAAAGCTATCAGGAATGGATCAAGTTCTCTCTGTGACTCCAAATAGGTATCACAAGCTGCACACGACAAGGTCATGGAACTTCATTGGGTTACCATTAAAAGCTAGAAGAGATTTAAAAAGTGAAAGTGACATTATAGTTGGTCTCTTTGATACAGGAATTACTCCAGAGTCCCAAAGCTTTACGGATGAAGGCTTTAGTCCTCCACCAGCAAAATGGAAAGGAACTTGTGGCCGGTTTGCTAATTTCTCAGGATGCAACAACAAAATCATAGGAGCTAGATACTTCAAGCTTGATGGCACTCCAGACCCAGCTGACATCTTATCTCCGGTGGATGTGGATGGACATGGGACTCACACATCATCGACCTTAGCTGGCAACCTAGTGCCGAATGCCAACCTCTATGGCCTTGCTAAGGGCACTGCACGAGGGGCAGTGCCATCGGCTAGGGTGTCCATGTACAAAGTATGTTGGGCTAGTTTTGGCTGCTCTGACATGGACATTCTTGCTGCGTATGAAGCTGCCATACACGATGGCGTGGATGTCATATCGATTTCCATTGGTGGAGGGGGAGCCTCTTATACTTTGGATAGTATAGCAGTTGGTGCATTTCATGCCATGAAGAATGGTATTATTACAGTGGCCTCAGCTGGAAATGATGGACCAAGTTTGGGGAGTATTGTCAACCATGCACCATGGTTGGTAACTGTGGCAGCTAGTGGCATTGATAGGCAGTTCATTAGCAAAGTTCATTTGGGCAATGGGAAGAATTTTGCAGGGATTGGAGTGAGCACATTTAATCCAAATAAGACGTTGTACCCTCTTGTTAGTGGGGCTGATGTAGCACAAAGCTCGGAAAGCAAAGAAAATGCAAGGTTCTGTGTTGAGGACTCATTAGACCCTAAAAAAGTGAAGGGAAAGATTGTTTTCTGCCAATTTAGATCTTGGGGTACAGATTCTGTCATTAAAGGAAATGGAGGAGTTGGTACCATTGTTGAAAATGATCAATTTCTCGATGCTGCTCAAATATTCATGGCACCAGGAACCATGGTTAACTCTACAATTGGCAAAAGCATTGATGCCTATATACATTCAGCAAGATCACCATCAGCAGTTATATTCAAGTCACAAGAAGCACAAAAACGTGCTCCATATGTTGCTTCATTTTCATCCAGGGGTCCAAATCCGGGATCAGAACACATTCTAAAGCCTGATATTGCAGCACCTGGGATTGACATTTTGGCATCTTACACTCCATTGAAGTCACTTACTGGGTTGGATGGTGATACGCAATACTCAGAATTCACACTCATGTCTGGAACTTCAATGGCATGTCCACATGTTGCTGGTGTAGCGGCATATGTTAAGTCATTTCACCCGAATTGGTCTCAAGCTGCAATAAGATCTGCCATCATGACCACAGCAACACCGATGAGCCATAGAGTGAACACAGAAGCAGAATTCGCCTATGGTGCTGGTCAAGTGAACCCGAGAAAAGCTGTGAATCCAGGCTTAATCTATGACATGGATGAAATGTCATACATTCAGTTCCTATGCCATGAAGGCTATAGTGGCTCCTCATTAGCCCCATTAGTTGGCTCAAAGTCCATAAACTGCTCCTCGTTGATTCCTGGACTCGGCTACGATGCTCTCAACTATCCCACCATGCAGCTCAGCTTGAAAAGCAGTAAGCAACCGACAGTAGCTGTTTTCAGAAGAACTGTCACTAATGTTGGTCCTGCTAGTTCCACATACAATGCCACCATCAGAGCACCAAAGGGAGTGGAAATCACTGTCAAACCCATGAGTCTGACTTTCTCTCGTGCTTTGCAAAAGAGGAGCTTCCAAGTTGTGGTGAAGGCGAATCCAATGGACAGTATGCAAATGGTGTCCGGCTCGCTCGGATGGAAAAGCTCTAGCTCTTCAAGTTGGAAATTTTCATCCCAAATATACCATTCCATTCTTTGTTGTAGAGGTAACATGATGGTTAGAGCATTCTTGTTGTTCTTGGCTTTCATGGCTACAACTAGAACAGCACTGATGGAAACACAGACGTACATTGTTCACATGGACAAGGCGAAAATCCCTGCCTGGGGTCACAAAGATCAACACTACTACTACAAAGCAGTGATGGATTCCATTACCGAACTCTCATCTCAAGAAGAAGAGGAAGAAGAAGGTGGCAAAGCATCAGCTTCTCAGCTTCTTTACATCTACAACACTGCTATTTCCGGTTTTGCAGCAAAGCTCTCAAGCAATCAACTCAAATCCTTGAGCCAAATCCATGGTTTCCTAGATGCCACACCTGATGAGCTTCTAACTCTCCACACCACACACACTCCTCACTTTCTTGGCTTACAAGAAGGAAAGGGACTTTGGAGTGCTCCCAACTTGGCTACGGATGTGATCATTGGGGTTGTTGACACAGGAGTCTGGCCAGAACATATTAGCTTCAAGGACAAAGGAATGTCCTCTGTACCCTCTCACTGGAATGGCACTTGTGAACAAGGCACAAGGTTCTCACACTCAAATTGTAACAAAAAGCTTATTGGTGCAAGAGCCTTTTTCCAAGGGTATGAGGCCATTGTCGGAAGAGTCAATGAAACAGTGGATTATAGATCCCCTCGTGACTCCGAAGGACATGGAACTCACACTGCTTCAACCGCAGGCGGTAATGTTGTCAAACAAGCAAGCTTATTTGGCTTAGCAAAGGGATCGGCCAGTGGAATGAGACATTCTTCAAGAATTGCAGCCTATAAGGCCTGTTGGCCATTGGGGTGTGCTAACTCTGATGTAATGGCTGCTATTGACAGTGCTGTTTCTGATGGGGTAGACATTTTGTCACTCTCATTGGGAGGTCTTCCGAGACCTTATTTTAGTGACAATATTGCTATAGCTTCATTTGGTGCAGTCCAACACGGAGTTTCCGTGTCATCTTCAGCTGGAAATTCAGGTCCCTTTCGCTCAAGCGTTAGCAATACCGCACCTTGGATCATGACTGTTGCTGCTAGCTACACTGACCGAAGCTTCCCAGTGATAGTGAAGCTTGGGAATGGACAAACTTATGAAGGGTCATCACTATATTCAGGAAAGAAAACTAAGCAGTTGCCACTAGTGTATAACAGAACAGCGGGCGGCCAAGGAGCTGAGCATTGTGTCGATGGCTCGCTTGACAGAAAGCTTGTGAAGGGAAAGATTGTAGCATGTGAGCGAGGAACACACAGCCGGACTGAAATTGGAGAGGAAGTGAAGAAAGCAGGAGGAGCCGGAATGCTTCTACTCAATAATGAAGCTCAAGGAGAGGAACTACTTGCTGATGCTCACATTTTGCCAGCCACTTCTCTTGGAGCTTCGGCTTCTAATGCCGTTAGAAAATACGCAGGTGCAGCAAAGAAACCTACAGCTTTGCTTCTGTTCCAAGGCACGGTCTTTGGTAAAACTGCTCCTGTGATGGCAGCATTTTCATCTAGGGGGCCAAGCTCAGTTGGACCGGGCGTGATCAAGCCCGATGTGACTGCTCCTGGTGTCAACATTTTGGCTGCTTGGCCAGCCAAGACTAGCCCAACCAGGCTTAAAAGTGACAAGAGAAAGGTGTTGTTTAATATGGTCTCAGGCACTTCAATGTCTTGCCCTCATGTTAGTGGCTTAGCTGCTCTTATCAAATCAGTGCACAGAGATTGGTCATCTGCAGCAATCAAATCTGCACTTATGACTACAGCTTATACACTAAACAACAAAGGCTCTCCGATTTCTGATTTTGGCTCAAAAAATCAATCAGCTAACCCTTTTGCATTTGGTTCGGGGCATGTCGACCCAGAAAGTGCTGCCGATCCAGGACTAATCTATGATATCACCAGCAAAGATTACCTGCTCTATTTATGTAGCTTGAATTACACATCATCCCAAATAGCTCTTTTCTCCAGTGGGATTAGTAACTTCACTTGTCCTAGCAATAGTGCAGTTCTTCTTCAGCCGGGCGACTTGAACTACCCTTCATTCTCAGTGGTTTTCCAAAAAGGTGGAAGGAAAATGAGTGTGACATACGAGAGGACAGTGACAAATGTCGGTGCCATTAACCCTAGTACTTATGCACTGCAAGTGGAAGCACCCATTGGAGTATCAGTTACTGTTGAGCCTAAGAAATTGGTTTTTAATAAGATGGGTGAGAAATTGAGCTACAAGGTGAGTTTTGCTGGATTGAGTACTATAACCGATTCCTCATTTGGATCCTTAGTTTGGGTTCTGATCAAAGCTCATTTTCTGAGAATTATAAAAATCTGCAATGTTCTATTGTTTGCCATAGAACTATTTGATCCTGCTGCTATGGCAATTGATAACTTGACGGACTCTTGGCCAAACTTGTGGAAAGTAAAAACCCTCTCCGCTCCCGCTACCGCAATTCCGCCGCCGTCGCCTGCCGCACCGGAGATTCTGCTCCGCCTCCATTTAGAGTGTTTGAATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

1582

Amino Acids

168.61

Weight (kDa)

8.79

Isoelectric Point (pI)

34.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 36 - 114 1.9e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 586 6.4e-45 Subtilase family
fn3_6 PF17766 644 - 733 1.1e-22 Fibronectin type-III domain
Inhibitor_I9 PF05922 778 - 869 1.3e-10 Peptidase inhibitor I9
Peptidase_S8 PF00082 894 - 1347 2.6e-47 Subtilase family
PA PF02225 1128 - 1214 3.2e-10 PA domain
fn3_6 PF17766 1420 - 1512 1.3e-23 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00230 AT4G00230
fragaria_vesca FvH4_7g12740
malus_domestica MD02G1196900.v1.1
prunus_persica Prupe.2G151900_v2.0.a1
pyrus_communis pycom02g16020
rosa_chinensis RchiOBHm_Chr1g0351761
rosa_laevigata RLG00000028385
rosa_multiflora Rmu_sc0001933.1_g000013
rosa_roxburghii Rroxscaffold_4G00303570
rosa_rugosa Rorug01G0221200
rosa_samantha Rh1AG235200 Rh1BG205700 Rh1CG219800 Rh1DG232700
rosa_wichuraiana Rw1G020470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4550
AarI CACCTGC 1 cut(s) 3650
AasI GACNNNNNNGTC 1 cut(s) 2708
Acc36I ACCTGC 2 cut(s) 3650, 4155
Acc65I GGTACC 1 cut(s) 1265
AccB1I GGYRCC 3 cut(s) 1265, 1315, 4346
AccB7I CCANNNNNTGG 2 cut(s) 1874, 2131
AccBSI CCGCTC 1 cut(s) 4664
AccI GTMKAC 2 cut(s) 3111, 4089
AcoI YGGCCR 7 cut(s) 511, 2714, 2997, 3047, 3415, 3824, 4631
AcsI RAATTY 5 cut(s) 1048, 1580, 1739, 2213, 3226
AcuI CTGAAG 2 cut(s) 3201, 4232
AfaI GTAC 8 cut(s) 740, 1097, 1231, 1267, 2336, 2757, 4363, 4483
AflIII ACRYGT 1 cut(s) 1618
AhdI GACNNNNNGTC 1 cut(s) 3118
AhlI ACTAGT 1 cut(s) 3391
AjnI CCWGG 9 cut(s) 1318, 1451, 1504, 1786, 1909, 2369, 3799, 3845, 4111
AjuI GAANNNNNNNTTGG 4 cut(s) 2638, 2670, 4374, 4406
Alw21I GWGCWC 5 cut(s) 1073, 1429, 2053, 2665, 3951
Alw26I GTCTC 6 cut(s) 7, 428, 1671, 3004, 3139, 3892
Alw44I GTGCAC 1 cut(s) 3947
AlwNI CAGNNNCTG 4 cut(s) 1238, 1505, 1985, 4406
Ama87I CYCGRG 1 cut(s) 1768
ApaLI GTGCAC 1 cut(s) 3947
ApoI RAATTY 5 cut(s) 1048, 1580, 1739, 2213, 3226
Asp700I GAANNNNTTC 2 cut(s) 196, 1479
Asp718I GGTACC 1 cut(s) 1265
AspS9I GGNCC 6 cut(s) 941, 1457, 2015, 3233, 3752, 3767
AsuC2I CCSGG 3 cut(s) 1467, 3771, 4254
AsuHPI GGTGA 8 cut(s) 119, 1374, 1574, 1646, 2146, 4123, 4454, 4474
AsuNHI GCTAGC 1 cut(s) 3287
AvaI CYCGRG 1 cut(s) 1768
AvaII GGWCC 5 cut(s) 941, 1457, 2015, 3233, 3767
BaeGI GKGCMC 2 cut(s) 701, 3951
BaeI ACNNNNGTAYC 2 cut(s) 1257, 1290
BalI TGGCCA 4 cut(s) 2716, 3049, 3826, 4633
BamHI GGATCC 1 cut(s) 4505
BanI GGYRCC 3 cut(s) 1265, 1315, 4346
BarI GAAGNNNNNNTAC 2 cut(s) 789, 821
BauI CACGAG 3 cut(s) 705, 2100, 2904
BbsI GAAGAC 2 cut(s) 150, 3131
Bbv12I GWGCWC 5 cut(s) 1073, 1429, 2053, 2665, 3951
BbvCI CCTCAGC 1 cut(s) 925
BceAI ACGGC 2 cut(s) 3629, 4672
BciT130I CCWGG 9 cut(s) 1320, 1453, 1506, 1788, 1911, 2371, 3801, 3847, 4113
BclI TGATCA 5 cut(s) 136, 1282, 2685, 3777, 4524
BcnI CCSGG 3 cut(s) 1467, 3771, 4254
BcoDI GTCTC 6 cut(s) 7, 428, 1671, 3004, 3139, 3892
BcuI ACTAGT 1 cut(s) 3391
BfmI CTRYAG 7 cut(s) 150, 207, 1846, 3171, 3678, 3966, 3993
BfuAI ACCTGC 2 cut(s) 3650, 4155
BglI GCCNNNNNGGC 2 cut(s) 1842, 3038
BglII AGATCT 2 cut(s) 1220, 1682
BlpI GCTNAGC 5 cut(s) 258, 1952, 2983, 3426, 3925
BmcAI AGTACT 2 cut(s) 4363, 4483
Bme18I GGWCC 5 cut(s) 941, 1457, 2015, 3233, 3767
BmeRI GACNNNNNGTC 1 cut(s) 3118
BmeT110I CYCGRG 1 cut(s) 1768
BmgT120I GGNCC 6 cut(s) 941, 1457, 2015, 3233, 3752, 3767
BmrI ACTGGG 2 cut(s) 1560, 3305
BmsI GCATC 8 cut(s) 524, 1285, 1348, 1532, 1915, 2477, 2566, 3586
BmtI GCTAGC 1 cut(s) 3291
BmuI ACTGGG 2 cut(s) 1560, 3305
BoxI GACNNNNGTC 1 cut(s) 4340
BpiI GAAGAC 2 cut(s) 150, 3131
BpmI CTGGAG 3 cut(s) 429, 567, 4183
Bpu10I CCTNAGC 3 cut(s) 649, 693, 925
Bpu1102I GCTNAGC 5 cut(s) 258, 1952, 2983, 3426, 3925
BpuEI CTTGAG 6 cut(s) 1653, 2421, 2515, 2572, 3229, 3558
BpuMI CCSGG 3 cut(s) 1467, 3771, 4254
Bsa29I ATCGAT 1 cut(s) 830
BsaBI GATNNNNATC 1 cut(s) 1384
BsaI GGTCTC 4 cut(s) 428, 1671, 3139, 3892
BsaWI WCCGGW 3 cut(s) 607, 2510, 4702
BsaXI ACNNNNNCTCC 8 cut(s) 2602, 2632, 3192, 3222, 3510, 3540, 4386, 4416
Bse118I RCCGGY 1 cut(s) 513
Bse1I ACTGG 6 cut(s) 267, 1555, 2771, 3000, 3311, 4200
Bse3DI GCAATG 2 cut(s) 1045, 4567
Bse8I GATNNNNATC 1 cut(s) 1384
BseBI CCWGG 9 cut(s) 1320, 1453, 1506, 1788, 1911, 2371, 3801, 3847, 4113
BseCI ATCGAT 1 cut(s) 830
BseJI GATNNNNATC 1 cut(s) 1384
BseMI GCAATG 2 cut(s) 1045, 4567
BseNI ACTGG 6 cut(s) 267, 1555, 2771, 3000, 3311, 4200
BseRI GAGGAG 6 cut(s) 1272, 1846, 1888, 2131, 2610, 3555
BseSI GKGCMC 2 cut(s) 701, 3951
BseYI CCCAGC 1 cut(s) 589
BsgI GTGCAG 6 cut(s) 323, 687, 3207, 3684, 3966, 4257
BshNI GGYRCC 3 cut(s) 1265, 1315, 4346
BshVI ATCGAT 1 cut(s) 830
BsiHKAI GWGCWC 5 cut(s) 1073, 1429, 2053, 2665, 3951
BsiHKCI CYCGRG 1 cut(s) 1768
BslFI GGGAC 4 cut(s) 436, 643, 2664, 3219
BsmAI GTCTC 6 cut(s) 7, 428, 1671, 3004, 3139, 3892
BsmFI GGGAC 4 cut(s) 436, 643, 2664, 3219
BsmI GAATGC 3 cut(s) 676, 2273, 3555
Bso31I GGTCTC 4 cut(s) 428, 1671, 3139, 3892
BsoBI CYCGRG 1 cut(s) 1768
Bsp1286I GDGCHC 6 cut(s) 701, 1073, 1429, 2053, 2665, 3951
Bsp1407I TGTACA 1 cut(s) 738
Bsp1720I GCTNAGC 5 cut(s) 258, 1952, 2983, 3426, 3925
Bsp19I CCATGG 4 cut(s) 216, 974, 1326, 2563
BspDI ATCGAT 1 cut(s) 830
BspHI TCATGA 3 cut(s) 139, 1692, 3273
BspMAI CTGCAG 2 cut(s) 154, 3970
BspMI ACCTGC 2 cut(s) 3650, 4155
BspOI GCTAGC 1 cut(s) 3291
BspQI GCTCTTC 1 cut(s) 2206
BspT107I GGYRCC 3 cut(s) 1265, 1315, 4346
BspTNI GGTCTC 4 cut(s) 428, 1671, 3139, 3892
BsrBI CCGCTC 1 cut(s) 4664
BsrDI GCAATG 2 cut(s) 1045, 4567
BsrFI RCCGGY 1 cut(s) 513
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 6 cut(s) 267, 1555, 2771, 3000, 3311, 4200
BssAI RCCGGY 1 cut(s) 513
BssSI CACGAG 3 cut(s) 705, 2100, 2904
BssT1I CCWWGG 8 cut(s) 216, 974, 1326, 2563, 2848, 3266, 3418, 3697
Bst2BI CACGAG 3 cut(s) 705, 2100, 2904
Bst2UI CCWGG 9 cut(s) 1320, 1453, 1506, 1788, 1911, 2371, 3801, 3847, 4113
Bst6I CTCTTC 4 cut(s) 382, 2206, 2439, 2866
BstAPI GCANNNNNTGC 3 cut(s) 254, 884, 893
BstAUI TGTACA 1 cut(s) 738
BstDSI CCRYGG 4 cut(s) 216, 974, 1326, 2563
BstEII GGTNACC 1 cut(s) 369
BstENI CCTNNNNNAGG 1 cut(s) 692
BstMAI GTCTC 6 cut(s) 7, 428, 1671, 3004, 3139, 3892
BstNI CCWGG 9 cut(s) 1320, 1453, 1506, 1788, 1911, 2371, 3801, 3847, 4113
BstNSI RCATGY 4 cut(s) 1614, 1622, 3486, 4088
BstPAI GACNNNNGTC 1 cut(s) 4340
BstPI GGTNACC 1 cut(s) 369
BstSFI CTRYAG 7 cut(s) 150, 207, 1846, 3171, 3678, 3966, 3993
BstSLI GKGCMC 2 cut(s) 701, 3951
BstV2I GAAGAC 2 cut(s) 150, 3131
BstX2I RGATCY 4 cut(s) 1220, 1682, 2897, 4505
BstXI CCANNNNNNTGG 4 cut(s) 495, 2564, 4298, 4641
BstYI RGATCY 4 cut(s) 1220, 1682, 2897, 4505
Bsu15I ATCGAT 1 cut(s) 830
BsuTUI ATCGAT 1 cut(s) 830
BtgI CCRYGG 4 cut(s) 216, 974, 1326, 2563
BtsI GCAGTG 6 cut(s) 699, 720, 2412, 2499, 2931, 4370
BveI ACCTGC 2 cut(s) 3650, 4155
CaiI CAGNNNCTG 4 cut(s) 1238, 1505, 1985, 4406
CciI TCATGA 3 cut(s) 139, 1692, 3273
Cfr10I RCCGGY 1 cut(s) 513
Cfr13I GGNCC 6 cut(s) 941, 1457, 2015, 3233, 3752, 3767
ClaI ATCGAT 1 cut(s) 830
Csp6I GTAC 8 cut(s) 739, 1096, 1230, 1266, 2335, 2756, 4362, 4482
CspCI CAANNNNNGTGG 4 cut(s) 2043, 2078, 3604, 3639
CviQI GTAC 8 cut(s) 739, 1096, 1230, 1266, 2335, 2756, 4362, 4482
DraI TTTAAA 1 cut(s) 396
DrdI GACNNNNNNGTC 1 cut(s) 2708
DriI GACNNNNNGTC 1 cut(s) 3118
DseDI GACNNNNNNGTC 1 cut(s) 2708
EaeI YGGCCR 7 cut(s) 511, 2714, 2997, 3047, 3415, 3824, 4631
Eam1104I CTCTTC 4 cut(s) 382, 2206, 2439, 2866
Eam1105I GACNNNNNGTC 1 cut(s) 3118
EarI CTCTTC 4 cut(s) 382, 2206, 2439, 2866
EciI GGCGGA 2 cut(s) 4671, 4706
Eco130I CCWWGG 8 cut(s) 216, 974, 1326, 2563, 2848, 3266, 3418, 3697
Eco147I AGGCCT 2 cut(s) 63, 3041
Eco31I GGTCTC 4 cut(s) 428, 1671, 3139, 3892
Eco32I GATATC 1 cut(s) 4129
Eco47I GGWCC 5 cut(s) 941, 1457, 2015, 3233, 3767
Eco57I CTGAAG 2 cut(s) 3201, 4232
Eco88I CYCGRG 1 cut(s) 1768
Eco91I GGTNACC 1 cut(s) 369
EcoNI CCTNNNNNAGG 1 cut(s) 692
EcoO109I RGGNCCY 1 cut(s) 3233
EcoO65I GGTNACC 1 cut(s) 369
EcoRI GAATTC 2 cut(s) 1580, 1739
EcoRII CCWGG 9 cut(s) 1318, 1451, 1504, 1786, 1909, 2369, 3799, 3845, 4111
EcoRV GATATC 1 cut(s) 4129
EcoT14I CCWWGG 8 cut(s) 216, 974, 1326, 2563, 2848, 3266, 3418, 3697
EcoT22I ATGCAT 1 cut(s) 250
ErhI CCWWGG 8 cut(s) 216, 974, 1326, 2563, 2848, 3266, 3418, 3697
FalI AAGNNNNNCTT 2 cut(s) 2105, 2137
FaqI GGGAC 4 cut(s) 436, 643, 2664, 3219
FauI CCCGC 2 cut(s) 3403, 4675
FauNDI CATATG 4 cut(s) 48, 165, 1432, 1639
FbaI TGATCA 5 cut(s) 136, 1282, 2685, 3777, 4524
FblI GTMKAC 2 cut(s) 3111, 4089
GsaI CCCAGC 1 cut(s) 593
GsuI CTGGAG 3 cut(s) 429, 567, 4183
HincII GTYRAC 5 cut(s) 964, 1333, 2701, 3808, 4090
HindII GTYRAC 5 cut(s) 964, 1333, 2701, 3808, 4090
HindIII AAGCTT 7 cut(s) 457, 569, 2822, 2971, 3304, 3323, 3458
HpaI GTTAAC 1 cut(s) 1333
HphI GGTGA 8 cut(s) 119, 1374, 1574, 1646, 2146, 4123, 4454, 4474
Hpy99I CGWCG 1 cut(s) 4693
HpyCH4IV ACGT 3 cut(s) 1091, 1423, 2333
HpySE526I ACGT 3 cut(s) 1091, 1423, 2333
KpnI GGTACC 1 cut(s) 1269
Ksp22I TGATCA 5 cut(s) 136, 1282, 2685, 3777, 4524
KspAI GTTAAC 1 cut(s) 1333
LguI GCTCTTC 1 cut(s) 2206
LweI GCATC 8 cut(s) 524, 1285, 1348, 1532, 1915, 2477, 2566, 3586
MaeII ACGT 3 cut(s) 1091, 1423, 2333
MbiI CCGCTC 1 cut(s) 4664
MfeI CAATTG 2 cut(s) 1341, 4608
MflI RGATCY 4 cut(s) 1220, 1682, 2897, 4505
MhlI GDGCHC 6 cut(s) 701, 1073, 1429, 2053, 2665, 3951
MlsI TGGCCA 4 cut(s) 2716, 3049, 3826, 4633
MluNI TGGCCA 4 cut(s) 2716, 3049, 3826, 4633
MmeI TCCRAC 5 cut(s) 1536, 2189, 2848, 3217, 3745
Mox20I TGGCCA 4 cut(s) 2716, 3049, 3826, 4633
Mph1103I ATGCAT 1 cut(s) 250
MroXI GAANNNNTTC 2 cut(s) 196, 1479
MscI TGGCCA 4 cut(s) 2716, 3049, 3826, 4633
MslI CAYNNNNRTG 6 cut(s) 168, 1589, 2769, 3055, 3899, 3918
Msp20I TGGCCA 4 cut(s) 2716, 3049, 3826, 4633
MspA1I CMGCKG 4 cut(s) 593, 929, 3221, 3410
MunI CAATTG 2 cut(s) 1341, 4608
Mva1269I GAATGC 3 cut(s) 676, 2273, 3555
MvaI CCWGG 9 cut(s) 1320, 1453, 1506, 1788, 1911, 2371, 3801, 3847, 4113
NciI CCSGG 3 cut(s) 1467, 3771, 4254
NcoI CCATGG 4 cut(s) 216, 974, 1326, 2563
NdeI CATATG 4 cut(s) 48, 165, 1432, 1639
NheI GCTAGC 1 cut(s) 3287
NmeAIII GCCGAG 1 cut(s) 1896
NsiI ATGCAT 1 cut(s) 250
NspI RCATGY 4 cut(s) 1614, 1622, 3486, 4088
PagI TCATGA 3 cut(s) 139, 1692, 3273
PaqCI CACCTGC 1 cut(s) 3650
PceI AGGCCT 2 cut(s) 63, 3041
PciI ACATGT 1 cut(s) 1618
PciSI GCTCTTC 1 cut(s) 2206
PcsI WCGNNNNNNNCGW 1 cut(s) 3204
PctI GAATGC 3 cut(s) 676, 2273, 3555
PdmI GAANNNNTTC 2 cut(s) 196, 1479
PfeI GAWTC 7 cut(s) 1235, 1783, 1906, 2143, 2414, 4493, 4708
PflFI GACNNNGTC 1 cut(s) 349
PflMI CCANNNNNTGG 2 cut(s) 1874, 2131
PfoI TCCNGGA 3 cut(s) 1465, 1909, 4111
PpuMI RGGWCCY 1 cut(s) 3233
PscI ACATGT 1 cut(s) 1618
PshAI GACNNNNGTC 1 cut(s) 4340
PsiI TTATAA 1 cut(s) 4550
Psp5II RGGWCCY 1 cut(s) 3233
Psp6I CCWGG 9 cut(s) 1318, 1451, 1504, 1786, 1909, 2369, 3799, 3845, 4111
PspEI GGTNACC 1 cut(s) 369
PspFI CCCAGC 1 cut(s) 589
PspGI CCWGG 9 cut(s) 1318, 1451, 1504, 1786, 1909, 2369, 3799, 3845, 4111
PspPI GGNCC 6 cut(s) 941, 1457, 2015, 3233, 3752, 3767
PspPPI RGGWCCY 1 cut(s) 3233
PstI CTGCAG 2 cut(s) 154, 3970
PstNI CAGNNNCTG 4 cut(s) 1238, 1505, 1985, 4406
PsuI RGATCY 4 cut(s) 1220, 1682, 2897, 4505
PsyI GACNNNGTC 1 cut(s) 349
PvuII CAGCTG 3 cut(s) 593, 929, 3221
RsaI GTAC 8 cut(s) 740, 1097, 1231, 1267, 2336, 2757, 4363, 4483
RsaNI GTAC 8 cut(s) 739, 1096, 1230, 1266, 2335, 2756, 4362, 4482
RseI CAYNNNNRTG 6 cut(s) 168, 1589, 2769, 3055, 3899, 3918
SalI GTCGAC 1 cut(s) 4088
SapI GCTCTTC 1 cut(s) 2206
Sau96I GGNCC 6 cut(s) 941, 1457, 2015, 3233, 3752, 3767
ScaI AGTACT 2 cut(s) 4363, 4483
SduI GDGCHC 6 cut(s) 701, 1073, 1429, 2053, 2665, 3951
SfaNI GCATC 8 cut(s) 524, 1285, 1348, 1532, 1915, 2477, 2566, 3586
SfcI CTRYAG 7 cut(s) 150, 207, 1846, 3171, 3678, 3966, 3993
SinI GGWCC 5 cut(s) 941, 1457, 2015, 3233, 3767
SmiMI CAYNNNNRTG 6 cut(s) 168, 1589, 2769, 3055, 3899, 3918
SmlI CTYRAG 6 cut(s) 1668, 2436, 2530, 2551, 3244, 3573
SmoI CTYRAG 6 cut(s) 1668, 2436, 2530, 2551, 3244, 3573
SpeI ACTAGT 1 cut(s) 3391
SseBI AGGCCT 2 cut(s) 63, 3041
SspI AATATT 2 cut(s) 1308, 3166
StuI AGGCCT 2 cut(s) 63, 3041
StyI CCWWGG 8 cut(s) 216, 974, 1326, 2563, 2848, 3266, 3418, 3697
TaiI ACGT 3 cut(s) 1094, 1426, 2336
TaqI TCGA 5 cut(s) 644, 830, 1293, 3438, 4089
TaqII GACCGA 1 cut(s) 3315
TatI WGTACW 3 cut(s) 738, 4361, 4481
TauI GCSGC 4 cut(s) 1637, 3417, 4687, 4700
TfiI GAWTC 7 cut(s) 1235, 1783, 1906, 2143, 2414, 4493, 4708
TspGWI ACGGA 5 cut(s) 479, 2693, 3196, 3213, 4637
Tth111I GACNNNGTC 1 cut(s) 349
Van91I CCANNNNNTGG 2 cut(s) 1874, 2131
VneI GTGCAC 1 cut(s) 3947
VpaK11BI GGWCC 5 cut(s) 941, 1457, 2015, 3233, 3767
XagI CCTNNNNNAGG 1 cut(s) 692
XapI RAATTY 5 cut(s) 1048, 1580, 1739, 2213, 3226
XceI RCATGY 4 cut(s) 1614, 1622, 3486, 4088
XcmI CCANNNNNNNNNTGG 1 cut(s) 492
XmiI GTMKAC 2 cut(s) 3111, 4089
XmnI GAANNNNTTC 2 cut(s) 196, 1479
ZrmI AGTACT 2 cut(s) 4363, 4483
Zsp2I ATGCAT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.