Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0014382

Physical Location & Seq

Genomic Coordinates Forward (+)

2128090 .. 2133150

Gene Structure 5061 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G04350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2922 bp

ATGAGCTCTCATCATCAAATCCTCCAAATTCGTTCTGACCCATTTGTTCTATCACACTGTTGCCGCCACACACGCCTCACCAGCTCTCTTACGCTTCAATCTCCTCTAAAGCAGCCCTTTTCATGTCTCCCTTTCCGTTGGCGCCGCTCTTATCGCGGCGGAGTCAGGAGCTCAACGACGGAGACTCATGGTAGCAAAAAAGAAGCTTTGGTGTCGGAAACGGCGACTACATCGATTGAATTGAAAAGGGTCTATCCATTTCACGAAATTGAACCCAAGTGGCAACGTTATTGGGAAGATAACCGAATTTTCCGGACACCAGACGATGTTGATACTTCGAAACCTAAGTTCTACGTCCTCGACATGTTTCCATATCCAAGTGGAGCAGGATTACATGTGGGACATCCATTGGGTTATACAGCAACTGATATTCTAGCGAGGCTTAGGCGTATGCAAGGTTACAATGTGTTGCATCCTATGGGTTGGGATGCTTTTGGATTGCCTGCTGAACAATATGCTATTGAGACAGGAACCCATCCGAAAACTACGACATTGAAGAACATTGACCGCTTTCGATTGCAGCTCAAATCATTGGGCTTCTCATACGATTGGGACCGTGAACTTTCTACAACAGAGCCAGACTACTACAAATGGACACAATGGATCTTTCTTCAGCTTTATAAGAAAGGTTTGGCATATCAGGCTGAAGTACCGGTCAATTGGTGCCCGGCTCTTGGTACTGTTTTGGCCAATGAAGAAGTGGTGGATGGTGTTAGTGAGCGTGGTGGCCACCCGGTTATAAGAAAGCCGATGAGGCAATGGATGCTGAAGATTACTGCGTACGCTGATCGTCTTCTAGAAGATTTGGACGAGCTTGAGTGGCCTGAAAGTATAAAGGAAATGCAAAGAAACTGGATAGGAAGATCCGAAGGAGCTGAGTTGAACTTTTCAATTCTTGATGGGGAAGGCCGAGAAACTGACAAAGAGATTACAGTGTACACCACGAGGCCAGATACACTTTTTGGAGCAACTTACATGGTTGTGGCACCAGAGCATCAGTTGTTGTCTTACTTTGTAACCGCAGAACAGAAACAGCAAGTTGAGGAATACAAAGATTTCGCTTCGAGGAAAAGTGATCTTGAGAGAACAGAACTTCAGAAGGACAAGACGGGCGTATTTACGGGATGTTATGCGAAAAATCCAGCTAATGGAGATGCTATTCCCATATGGGTAGCTGATTATGTTCTAGCTAGTTATGGAACCGGAGCAATCATGGCGGTCCCAGCTCATGATACTCGGGATAATGAGTTTGCGTTGAAGTATAACATTCCCATCAAGTGGGTAGTGAGAAATGAGGCAAATTCAAGTGATGATGCTAAGCAGGTTTATCCCGGATTGGGCATTATTGAAAATTCTTCAACTTTAGAAACAGGACTTGACATAAATCAACTATCTAGCAAAGAAGCTGCTTTAAAAGTTATTGAATGGGCTGAGAGAACTGGCAATGGAAAGAAAAAGGTAAATTACAAGTTGAGGGATTGGCTATTTGCACGGCAGCGTTACTGGGGTGAACCTATCCCGATTTTAATTTTGGATGAGTCTGGTGAAACTATTGCTATTTCAGAATCTGAACTGCCCCTCACTTTGCCTGAGTTAAATGATTTTACGCCCACTGGAACAGGGGAACCACCACTGTCAAAAGCAGTTTCATGGGTGAACACTGTAGATCCTTCAACAGGAAAACCTGCCAAAAGAGAAACAAGCACTATGCCACAATGGGCTGGGTCTTGCTGGTACTATCTGAGATTCATGGACCCGAAAAACCCTGAAGCATTAGTTGACAAGGAAAAAGAAAAATACTGGAGCCCAGTAGATGTATATGTTGGTGGTGCTGAGCATGCTGTTTTACATTTACTATACTCGAGGTTTTGGCACAAGGTACTTTATGACATTGGTGTTGTGTCTACGAAAGAACCATTCAAATGTGTCATAAATCAAGGAATCATCCTTGGGGAAGTCCAATATACAGCTTGGAAAGATCAAGAAGGAAATTATGTATCTGCAGATACTGAAGAACGGTTAAATGAACATCAACAAGTGACAATCCCGGAAGAAAAAGTTATCAAGTCCGGAGATCATTTTGTTCTGAAGGAGGATCCTAGCATTCGTCTGATTCCACGCGTTTATAAAATGAGCAAAAGTAGAGGAAATGTCGTAAATCCTGATGATGTTGTATTAGAGTATGGTGCAGATTCTCTACGTTTGTATGAGATGTTCATGGGACCATTTAGGGACTCAAAAACTTGGAACACGAGTGGGATTGAAGGTGTGCATCGTTTCTTGGCAAGAACTTGGAGACTAGTCATTGGTTTGCCTCAGTCTGATGGTTCTTTCAAAGATGGAACCTTAGTAACTGATGACGAACCAACCCTTGAACAACTCCGAACTCTTCATAAATGCATTGCAAAGGTGACAGAGGAAATCGAGAGTACACGATTCAACACGGGAATTTCAGGGATGATGGAGTTTGTTAATGCGGCATATAAGTGGAATAATCAACCTAGAGGAATCATTGAGCCTTTTGTTCTTTTGCTCTCACCTTACGCGCCTCACATGGCTGAAGAGCTTTGGTCGCGGTTAGGCCATCCAAATTCTCTGGCCTATGAAAGCTTCCCTAAGGCAAACCCGGATTACTTAAAGAACACAACTATTGTTCTTCCAGTTCAGATAAACGGCAAGACAAGAGGCACCATAGAGGTTGAAGAAGGATGCTCTGAAGATGATGCTTTTGTTCTAGCTTCACAGGACGACAAACTCAGGAAATATCTAGATGGACAATCAATCAAGAAGCGAATCTATGTGCCTGGGAAGATCCTCAACGTTATCCTAGACCGGACTAATGTCAAGGTCACTACAAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004823 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006429 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032543 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140053 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1 tRNA-synt_1_2

Protein Analysis

973

Amino Acids

111.03

Weight (kDa)

5.92

Isoelectric Point (pI)

40.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	94 - 306	1.9e-29	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	122 - 254	1.6e-17	tRNA synthetases class I (M)
tRNA-synt_1_2	PF13603	303 - 494	2e-59	Leucyl-tRNA synthetase, editing domain
Anticodon_1	PF08264	814 - 921	9.5e-16	Anticodon-binding domain of tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04350
fragaria_vesca	FvH4_3g01570 FvH4_3g01570
malus_domestica	MD10G1325600.v1.1
prunus_persica	Prupe.4G016400_v2.0.a1 Prupe.4G016400_v2.0.a1
pyrus_communis	pycom10g27530
rosa_chinensis	RchiOBHm_Chr5g0002381
rosa_laevigata	RLG00000031024
rosa_multiflora	Rmu_sc0006137.1_g000003 Rmu_sc0017945.1_g000003
rosa_roxburghii	Rroxscaffold_1G00073490
rosa_rugosa	Rorug04G0395800.1
rosa_samantha	Rh5AG018500 Rh5BG021200 Rh5DG019500
rosa_wichuraiana	Rw5G001740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	681, 800, 2187
Acc36I	ACCTGC	2 cut(s)	1372, 1753
AccB1I	GGYRCC	4 cut(s)	141, 723, 1045, 2747
AccB7I	CCANNNNNTGG	2 cut(s)	1208, 1338
AccBSI	CCGCTC	1 cut(s)	147
AccI	GTMKAC	1 cut(s)	1964
AccII	CGCG	4 cut(s)	156, 2181, 2606, 2635
AccIII	TCCGGA	2 cut(s)	312, 2129
AciI	CCGC	9 cut(s)	64, 145, 156, 159, 568, 1080, 1277, 2537, 2635
AclI	AACGTT	2 cut(s)	286, 2880
AclWI	GGATC	6 cut(s)	671, 918, 1721, 2150, 2163, 2866
AcoI	YGGCCR	2 cut(s)	747, 787
AcsI	RAATTY	6 cut(s)	27, 306, 1360, 1411, 2508, 2650
AcuI	CTGAAG	9 cut(s)	656, 726, 848, 1139, 1848, 2091, 2168, 2640, 2796
AcyI	GRCGYC	1 cut(s)	142
AfaI	GTAC	7 cut(s)	711, 739, 842, 998, 1796, 1941, 2491
AfiI	CCNNNNNNNGG	5 cut(s)	734, 1208, 1338, 1397, 1736
AflIII	ACRYGT	3 cut(s)	363, 394, 2179
AgeI	ACCGGT	1 cut(s)	712
AhlI	ACTAGT	1 cut(s)	2359
AjnI	CCWGG	1 cut(s)	2863
Alw21I	GWGCWC	2 cut(s)	8, 173
Alw26I	GTCTC	4 cut(s)	131, 176, 518, 2350
AlwI	GGATC	6 cut(s)	671, 918, 1721, 2150, 2163, 2866
AlwNI	CAGNNNCTG	3 cut(s)	425, 1628, 2069
Ama87I	CYCGRG	2 cut(s)	1296, 1921
Aor13HI	TCCGGA	2 cut(s)	312, 2129
AoxI	GGCC	7 cut(s)	747, 787, 881, 967, 1007, 2641, 2658
ApeKI	GCWGC	4 cut(s)	112, 580, 1466, 1555
ApoI	RAATTY	6 cut(s)	27, 306, 1360, 1411, 2508, 2650
AsiGI	ACCGGT	1 cut(s)	712
AspLEI	GCGC	2 cut(s)	144, 2608
AspS9I	GGNCC	4 cut(s)	613, 1279, 1813, 2282
AsuC2I	CCSGG	5 cut(s)	728, 794, 1392, 2108, 2687
AsuHPI	GGTGA	6 cut(s)	70, 1580, 1616, 1726, 2482, 2589
AsuII	TTCGAA	1 cut(s)	338
AvaI	CYCGRG	2 cut(s)	1296, 1921
AvaII	GGWCC	4 cut(s)	613, 1279, 1813, 2282
AxyI	CCTNAGG	1 cut(s)	2676
BaeGI	GKGCMC	1 cut(s)	728
BalI	TGGCCA	2 cut(s)	749, 789
BamHI	GGATCC	1 cut(s)	2155
BanI	GGYRCC	4 cut(s)	141, 723, 1045, 2747
BanII	GRGCYC	3 cut(s)	8, 173, 1868
BauI	CACGAG	2 cut(s)	1003, 2311
BbsI	GAAGAC	1 cut(s)	845
Bbv12I	GWGCWC	2 cut(s)	8, 173
BbvI	GCAGC	4 cut(s)	124, 592, 1453, 1567
BccI	CCATC	9 cut(s)	543, 761, 953, 1340, 2378, 2393, 2515, 2652, 2825
BceAI	ACGGC	3 cut(s)	237, 1568, 2749
BciT130I	CCWGG	1 cut(s)	2865
BcnI	CCSGG	5 cut(s)	728, 794, 1392, 2108, 2687
BcoDI	GTCTC	4 cut(s)	131, 176, 518, 2350
BcuI	ACTAGT	1 cut(s)	2359
BfmI	CTRYAG	2 cut(s)	1722, 2061
BfoI	RGCGCY	1 cut(s)	145
BfuAI	ACCTGC	2 cut(s)	1372, 1753
BglI	GCCNNNNNGGC	1 cut(s)	814
BisI	GCNGC	8 cut(s)	64, 113, 145, 157, 581, 1467, 1556, 2538
BlpI	GCTNAGC	2 cut(s)	1377, 1893
BlsI	GCNGC	8 cut(s)	65, 114, 146, 158, 582, 1468, 1557, 2539
Bme1390I	CCNGG	6 cut(s)	728, 794, 1392, 2108, 2687, 2865
Bme18I	GGWCC	4 cut(s)	613, 1279, 1813, 2282
BmeT110I	CYCGRG	2 cut(s)	1296, 1921
BmgT120I	GGNCC	4 cut(s)	613, 1279, 1813, 2282
BmrFI	CCNGG	6 cut(s)	728, 794, 1392, 2108, 2687, 2865
BmrI	ACTGGG	2 cut(s)	1573, 1862
BmsI	GCATC	9 cut(s)	478, 481, 813, 1063, 1204, 1363, 2341, 2759, 2773
BmuI	ACTGGG	2 cut(s)	1573, 1862
BoxI	GACNNNNGTC	1 cut(s)	2900
BpiI	GAAGAC	1 cut(s)	845
BpmI	CTGGAG	1 cut(s)	1882
Bpu10I	CCTNAGC	1 cut(s)	443
Bpu1102I	GCTNAGC	2 cut(s)	1377, 1893
Bpu14I	TTCGAA	1 cut(s)	338
BpuEI	CTTGAG	2 cut(s)	896, 1160
BpuMI	CCSGG	5 cut(s)	728, 794, 1392, 2108, 2687
Bsa29I	ATCGAT	1 cut(s)	233
BsaHI	GRCGYC	1 cut(s)	142
BsaJI	CCNNGG	2 cut(s)	2008, 2864
BsaWI	WCCGGW	5 cut(s)	312, 712, 1262, 2129, 2892
Bsc4I	CCNNNNNNNGG	5 cut(s)	734, 1208, 1338, 1397, 1736
Bse118I	RCCGGY	1 cut(s)	712
Bse1I	ACTGG	7 cut(s)	917, 1504, 1568, 1678, 1865, 1868, 2720
Bse21I	CCTNAGG	1 cut(s)	2676
Bse3DI	GCAATG	3 cut(s)	824, 1510, 2460
BseAI	TCCGGA	2 cut(s)	312, 2129
BseBI	CCWGG	1 cut(s)	2865
BseCI	ATCGAT	1 cut(s)	233
BseDI	CCNNGG	2 cut(s)	2008, 2864
BseLI	CCNNNNNNNGG	5 cut(s)	734, 1208, 1338, 1397, 1736
BseMI	GCAATG	3 cut(s)	824, 1510, 2460
BseMII	CTCAG	7 cut(s)	927, 1482, 1641, 1793, 1884, 2390, 2830
BseNI	ACTGG	7 cut(s)	917, 1504, 1568, 1678, 1865, 1868, 2720
BseRI	GAGGAG	1 cut(s)	93
BseSI	GKGCMC	1 cut(s)	728
BseXI	GCAGC	4 cut(s)	124, 592, 1453, 1567
BseYI	CCCAGC	2 cut(s)	1282, 1781
BsgI	GTGCAG	1 cut(s)	2268
Bsh1236I	CGCG	4 cut(s)	156, 2181, 2606, 2635
BshFI	GGCC	7 cut(s)	749, 789, 883, 969, 1009, 2643, 2660
BshNI	GGYRCC	4 cut(s)	141, 723, 1045, 2747
BshTI	ACCGGT	1 cut(s)	712
BshVI	ATCGAT	1 cut(s)	233
BsiHKAI	GWGCWC	2 cut(s)	8, 173
BsiHKCI	CYCGRG	2 cut(s)	1296, 1921
BsiWI	CGTACG	1 cut(s)	840
BslFI	GGGAC	5 cut(s)	414, 626, 1265, 2295, 2306
BslI	CCNNNNNNNGG	5 cut(s)	734, 1208, 1338, 1397, 1736
BsmAI	GTCTC	4 cut(s)	131, 176, 518, 2350
BsmFI	GGGAC	5 cut(s)	414, 626, 1265, 2295, 2306
BsmI	GAATGC	1 cut(s)	2163
BsnI	GGCC	7 cut(s)	749, 789, 883, 969, 1009, 2643, 2660
BsoBI	CYCGRG	2 cut(s)	1296, 1921
Bsp119I	TTCGAA	1 cut(s)	338
Bsp1286I	GDGCHC	4 cut(s)	8, 173, 728, 1868
Bsp13I	TCCGGA	2 cut(s)	312, 2129
Bsp1407I	TGTACA	1 cut(s)	996
Bsp143I	GATC	9 cut(s)	663, 847, 923, 1135, 1726, 2038, 2134, 2155, 2871
Bsp1720I	GCTNAGC	2 cut(s)	1377, 1893
BspACI	CCGC	9 cut(s)	64, 145, 156, 159, 568, 1080, 1277, 2537, 2635
BspANI	GGCC	7 cut(s)	749, 789, 883, 969, 1009, 2643, 2660
BspCNI	CTCAG	7 cut(s)	928, 1483, 1642, 1794, 1885, 2389, 2829
BspDI	ATCGAT	1 cut(s)	233
BspEI	TCCGGA	2 cut(s)	312, 2129
BspFNI	CGCG	4 cut(s)	156, 2181, 2606, 2635
BspHI	TCATGA	1 cut(s)	1288
BspMAI	CTGCAG	1 cut(s)	2065
BspMI	ACCTGC	2 cut(s)	1372, 1753
BspPI	GGATC	6 cut(s)	671, 918, 1721, 2150, 2163, 2866
BspQI	GCTCTTC	1 cut(s)	2616
BspT104I	TTCGAA	1 cut(s)	338
BspT107I	GGYRCC	4 cut(s)	141, 723, 1045, 2747
BsrBI	CCGCTC	1 cut(s)	147
BsrDI	GCAATG	3 cut(s)	824, 1510, 2460
BsrFI	RCCGGY	1 cut(s)	712
BsrGI	TGTACA	1 cut(s)	996
BsrI	ACTGG	7 cut(s)	917, 1504, 1568, 1678, 1865, 1868, 2720
BssAI	RCCGGY	1 cut(s)	712
BssECI	CCNNGG	2 cut(s)	2008, 2864
BssMI	GATC	9 cut(s)	663, 847, 923, 1135, 1726, 2038, 2134, 2155, 2871
BssNI	GRCGYC	1 cut(s)	142
BssSI	CACGAG	2 cut(s)	1003, 2311
BssT1I	CCWWGG	1 cut(s)	2008
Bst2BI	CACGAG	2 cut(s)	1003, 2311
Bst2UI	CCWGG	1 cut(s)	2865
Bst4CI	ACNGT	7 cut(s)	59, 617, 742, 994, 1695, 1723, 2079
Bst6I	CTCTTC	2 cut(s)	2454, 2616
BstACI	GRCGYC	1 cut(s)	142
BstAPI	GCANNNNNTGC	1 cut(s)	823
BstAUI	TGTACA	1 cut(s)	996
BstBI	TTCGAA	1 cut(s)	338
BstC8I	GCNNGC	2 cut(s)	504, 1899
BstENI	CCTNNNNNAGG	1 cut(s)	1734
BstFNI	CGCG	4 cut(s)	156, 2181, 2606, 2635
BstH2I	RGCGCY	1 cut(s)	145
BstHHI	GCGC	2 cut(s)	144, 2608
BstKTI	GATC	9 cut(s)	666, 850, 926, 1138, 1729, 2041, 2137, 2158, 2874
BstMAI	GTCTC	4 cut(s)	131, 176, 518, 2350
BstMBI	GATC	9 cut(s)	663, 847, 923, 1135, 1726, 2038, 2134, 2155, 2871
BstNI	CCWGG	1 cut(s)	2865
BstNSI	RCATGY	3 cut(s)	367, 398, 1901
BstPAI	GACNNNNGTC	1 cut(s)	2900
BstSCI	CCNGG	6 cut(s)	726, 792, 1390, 2106, 2685, 2863
BstSFI	CTRYAG	2 cut(s)	1722, 2061
BstSLI	GKGCMC	1 cut(s)	728
BstUI	CGCG	4 cut(s)	156, 2181, 2606, 2635
BstV1I	GCAGC	4 cut(s)	124, 592, 1453, 1567
BstV2I	GAAGAC	1 cut(s)	845
BstX2I	RGATCY	5 cut(s)	663, 923, 1726, 2155, 2871
BstYI	RGATCY	5 cut(s)	663, 923, 1726, 2155, 2871
Bsu15I	ATCGAT	1 cut(s)	233
Bsu36I	CCTNAGG	1 cut(s)	2676
BsuRI	GGCC	7 cut(s)	749, 789, 883, 969, 1009, 2643, 2660
BsuTUI	ATCGAT	1 cut(s)	233
BtsIMutI	CAGTG	5 cut(s)	55, 999, 1671, 1691, 1719
BveI	ACCTGC	2 cut(s)	1372, 1753
Cac8I	GCNNGC	2 cut(s)	504, 1899
CaiI	CAGNNNCTG	3 cut(s)	425, 1628, 2069
CciI	TCATGA	1 cut(s)	1288
CfoI	GCGC	2 cut(s)	144, 2608
Cfr10I	RCCGGY	1 cut(s)	712
Cfr13I	GGNCC	4 cut(s)	613, 1279, 1813, 2282
ClaI	ATCGAT	1 cut(s)	233
Csp6I	GTAC	7 cut(s)	710, 738, 841, 997, 1795, 1940, 2490
CspAI	ACCGGT	1 cut(s)	712
CviQI	GTAC	7 cut(s)	710, 738, 841, 997, 1795, 1940, 2490
DinI	GGCGCC	1 cut(s)	143
DpnI	GATC	9 cut(s)	665, 849, 925, 1137, 1728, 2040, 2136, 2157, 2873
DpnII	GATC	9 cut(s)	663, 847, 923, 1135, 1726, 2038, 2134, 2155, 2871
DraI	TTTAAA	1 cut(s)	1473
EaeI	YGGCCR	2 cut(s)	747, 787
Eam1104I	CTCTTC	2 cut(s)	2454, 2616
EarI	CTCTTC	2 cut(s)	2454, 2616
EciI	GGCGGA	1 cut(s)	174
Ecl136II	GAGCTC	2 cut(s)	6, 171
Eco130I	CCWWGG	1 cut(s)	2008
Eco24I	GRGCYC	3 cut(s)	8, 173, 1868
Eco47I	GGWCC	4 cut(s)	613, 1279, 1813, 2282
Eco53kI	GAGCTC	2 cut(s)	6, 171
Eco57I	CTGAAG	9 cut(s)	656, 726, 848, 1139, 1848, 2091, 2168, 2640, 2796
Eco81I	CCTNAGG	1 cut(s)	2676
Eco88I	CYCGRG	2 cut(s)	1296, 1921
EcoICRI	GAGCTC	2 cut(s)	6, 171
EcoNI	CCTNNNNNAGG	1 cut(s)	1734
EcoRII	CCWGG	1 cut(s)	2863
EcoT14I	CCWWGG	1 cut(s)	2008
EcoT22I	ATGCAT	1 cut(s)	2462
EcoT38I	GRGCYC	3 cut(s)	8, 173, 1868
EgeI	GGCGCC	1 cut(s)	143
EheI	GGCGCC	1 cut(s)	143
ErhI	CCWWGG	1 cut(s)	2008
FalI	AAGNNNNNCTT	2 cut(s)	101, 133
FaqI	GGGAC	5 cut(s)	414, 626, 1265, 2295, 2306
FauNDI	CATATG	1 cut(s)	1226
FblI	GTMKAC	1 cut(s)	1964
Fnu4HI	GCNGC	8 cut(s)	64, 113, 145, 157, 581, 1467, 1556, 2538
FriOI	GRGCYC	3 cut(s)	8, 173, 1868
Fsp4HI	GCNGC	8 cut(s)	64, 113, 145, 157, 581, 1467, 1556, 2538
GlaI	GCGC	2 cut(s)	143, 2607
GluI	GCNGC	8 cut(s)	64, 113, 145, 157, 581, 1467, 1556, 2538
GsaI	CCCAGC	2 cut(s)	1286, 1785
GsuI	CTGGAG	1 cut(s)	1882
HaeII	RGCGCY	1 cut(s)	145
HaeIII	GGCC	7 cut(s)	749, 789, 883, 969, 1009, 2643, 2660
HhaI	GCGC	2 cut(s)	144, 2608
Hin1I	GRCGYC	1 cut(s)	142
Hin6I	GCGC	2 cut(s)	142, 2606
HinP1I	GCGC	2 cut(s)	142, 2606
HincII	GTYRAC	1 cut(s)	1840
HindII	GTYRAC	1 cut(s)	1840
HindIII	AAGCTT	2 cut(s)	204, 2668
HphI	GGTGA	6 cut(s)	70, 1580, 1616, 1726, 2482, 2589
Hpy166II	GTNNAC	8 cut(s)	620, 997, 999, 1571, 1717, 1840, 1965, 2492
Hpy8I	GTNNAC	8 cut(s)	620, 997, 999, 1571, 1717, 1840, 1965, 2492
Hpy99I	CGWCG	1 cut(s)	181
HpyAV	CCTTC	8 cut(s)	923, 959, 1153, 1740, 2039, 2143, 2318, 2759
HpyCH4III	ACNGT	7 cut(s)	59, 617, 742, 994, 1695, 1723, 2079
HpyCH4IV	ACGT	4 cut(s)	286, 354, 2260, 2880
HpySE526I	ACGT	4 cut(s)	286, 354, 2260, 2880
Hsp92I	GRCGYC	1 cut(s)	142
HspAI	GCGC	2 cut(s)	142, 2606
KasI	GGCGCC	1 cut(s)	141
Kpn2I	TCCGGA	2 cut(s)	312, 2129
Kzo9I	GATC	9 cut(s)	663, 847, 923, 1135, 1726, 2038, 2134, 2155, 2871
LguI	GCTCTTC	1 cut(s)	2616
LmnI	GCTCC	6 cut(s)	168, 383, 932, 1025, 1265, 1863
Lsp1109I	GCAGC	4 cut(s)	124, 592, 1453, 1567
LweI	GCATC	9 cut(s)	478, 481, 813, 1063, 1204, 1363, 2341, 2759, 2773
MaeII	ACGT	4 cut(s)	286, 354, 2260, 2880
MaeIII	GTNAC	7 cut(s)	458, 1075, 1559, 2098, 2410, 2470, 2908
MalI	GATC	9 cut(s)	665, 849, 925, 1137, 1728, 2040, 2136, 2157, 2873
MbiI	CCGCTC	1 cut(s)	147
MboI	GATC	9 cut(s)	663, 847, 923, 1135, 1726, 2038, 2134, 2155, 2871
MfeI	CAATTG	1 cut(s)	718
MflI	RGATCY	5 cut(s)	663, 923, 1726, 2155, 2871
MhlI	GDGCHC	4 cut(s)	8, 173, 728, 1868
MlsI	TGGCCA	2 cut(s)	749, 789
MluI	ACGCGT	1 cut(s)	2179
MluNI	TGGCCA	2 cut(s)	749, 789
Mly113I	GGCGCC	1 cut(s)	142
MlyI	GAGTC	4 cut(s)	171, 178, 1607, 2288
MmeI	TCCRAC	1 cut(s)	195
Mox20I	TGGCCA	2 cut(s)	749, 789
Mph1103I	ATGCAT	1 cut(s)	2462
MroI	TCCGGA	2 cut(s)	312, 2129
MscI	TGGCCA	2 cut(s)	749, 789
MseI	TTAA	6 cut(s)	1472, 1586, 1655, 2081, 2532, 2696
MslI	CAYNNNNRTG	3 cut(s)	1040, 1981, 2545
Msp20I	TGGCCA	2 cut(s)	749, 789
MspR9I	CCNGG	6 cut(s)	728, 794, 1392, 2108, 2687, 2865
MunI	CAATTG	1 cut(s)	718
Mva1269I	GAATGC	1 cut(s)	2163
MvaI	CCWGG	1 cut(s)	2865
MvnI	CGCG	4 cut(s)	156, 2181, 2606, 2635
NarI	GGCGCC	1 cut(s)	142
NciI	CCSGG	5 cut(s)	728, 794, 1392, 2108, 2687
NdeI	CATATG	1 cut(s)	1226
NdeII	GATC	9 cut(s)	663, 847, 923, 1135, 1726, 2038, 2134, 2155, 2871
NmeAIII	GCCGAG	1 cut(s)	995
NmuCI	GTSAC	3 cut(s)	2098, 2470, 2908
NsiI	ATGCAT	1 cut(s)	2462
NspI	RCATGY	3 cut(s)	367, 398, 1901
NspV	TTCGAA	1 cut(s)	338
PaeI	GCATGC	1 cut(s)	1901
PaeR7I	CTCGAG	1 cut(s)	1921
PagI	TCATGA	1 cut(s)	1288
PciI	ACATGT	2 cut(s)	363, 394
PciSI	GCTCTTC	1 cut(s)	2616
PcsI	WCGNNNNNNNCGW	1 cut(s)	221
PctI	GAATGC	1 cut(s)	2163
PfeI	GAWTC	8 cut(s)	1625, 1806, 2001, 2173, 2252, 2496, 2568, 2853
Pfl23II	CGTACG	1 cut(s)	840
PflMI	CCANNNNNTGG	2 cut(s)	1208, 1338
PfoI	TCCNGGA	2 cut(s)	1390, 2106
PinAI	ACCGGT	1 cut(s)	712
PkrI	GCNGC	8 cut(s)	65, 114, 146, 158, 582, 1468, 1557, 2539
PleI	GAGTC	4 cut(s)	170, 178, 1606, 2288
PluTI	GGCGCC	1 cut(s)	145
PpsI	GAGTC	4 cut(s)	170, 178, 1606, 2288
PscI	ACATGT	2 cut(s)	363, 394
PshAI	GACNNNNGTC	1 cut(s)	2900
PsiI	TTATAA	3 cut(s)	681, 800, 2187
Psp124BI	GAGCTC	2 cut(s)	8, 173
Psp1406I	AACGTT	2 cut(s)	286, 2880
Psp6I	CCWGG	1 cut(s)	2863
PspFI	CCCAGC	2 cut(s)	1282, 1781
PspGI	CCWGG	1 cut(s)	2863
PspLI	CGTACG	1 cut(s)	840
PspPI	GGNCC	4 cut(s)	613, 1279, 1813, 2282
PspXI	VCTCGAGB	1 cut(s)	1921
PsrI	GAACNNNNNNTAC	2 cut(s)	2258, 2290
PstI	CTGCAG	1 cut(s)	2065
PstNI	CAGNNNCTG	3 cut(s)	425, 1628, 2069
PsuI	RGATCY	5 cut(s)	663, 923, 1726, 2155, 2871
RsaI	GTAC	7 cut(s)	711, 739, 842, 998, 1796, 1941, 2491
RsaNI	GTAC	7 cut(s)	710, 738, 841, 997, 1795, 1940, 2490
RseI	CAYNNNNRTG	3 cut(s)	1040, 1981, 2545
SacI	GAGCTC	2 cut(s)	8, 173
SapI	GCTCTTC	1 cut(s)	2616
SaqAI	TTAA	6 cut(s)	1472, 1586, 1655, 2081, 2532, 2696
SatI	GCNGC	8 cut(s)	64, 113, 145, 157, 581, 1467, 1556, 2538
Sau3AI	GATC	9 cut(s)	663, 847, 923, 1135, 1726, 2038, 2134, 2155, 2871
Sau96I	GGNCC	4 cut(s)	613, 1279, 1813, 2282
SchI	GAGTC	4 cut(s)	171, 178, 1607, 2288
ScrFI	CCNGG	6 cut(s)	728, 794, 1392, 2108, 2687, 2865
SduI	GDGCHC	4 cut(s)	8, 173, 728, 1868
SfaNI	GCATC	9 cut(s)	478, 481, 813, 1063, 1204, 1363, 2341, 2759, 2773
SfcI	CTRYAG	2 cut(s)	1722, 2061
SfoI	GGCGCC	1 cut(s)	143
Sfr274I	CTCGAG	1 cut(s)	1921
SfuI	TTCGAA	1 cut(s)	338
SinI	GGWCC	4 cut(s)	613, 1279, 1813, 2282
SlaI	CTCGAG	1 cut(s)	1921
SmiMI	CAYNNNNRTG	3 cut(s)	1040, 1981, 2545
SmlI	CTYRAG	3 cut(s)	875, 1139, 1921
SmoI	CTYRAG	3 cut(s)	875, 1139, 1921
SpeI	ACTAGT	1 cut(s)	2359
SphI	GCATGC	1 cut(s)	1901
SsiI	CCGC	9 cut(s)	64, 145, 156, 159, 568, 1080, 1277, 2537, 2635
SspDI	GGCGCC	1 cut(s)	141
SstI	GAGCTC	2 cut(s)	8, 173
StyD4I	CCNGG	6 cut(s)	726, 792, 1390, 2106, 2685, 2863
StyI	CCWWGG	1 cut(s)	2008
TaaI	ACNGT	7 cut(s)	59, 617, 742, 994, 1695, 1723, 2079
TaiI	ACGT	4 cut(s)	289, 357, 2263, 2883
TaqI	TCGA	7 cut(s)	233, 338, 360, 574, 1124, 1922, 2484
TatI	WGTACW	2 cut(s)	996, 2489
TauI	GCSGC	4 cut(s)	66, 147, 159, 2540
TfiI	GAWTC	8 cut(s)	1625, 1806, 2001, 2173, 2252, 2496, 2568, 2853
Tru1I	TTAA	6 cut(s)	1472, 1586, 1655, 2081, 2532, 2696
Tru9I	TTAA	6 cut(s)	1472, 1586, 1655, 2081, 2532, 2696
TscAI	CASTG	5 cut(s)	62, 999, 1678, 1698, 1726
TseFI	GTSAC	3 cut(s)	2098, 2470, 2908
TseI	GCWGC	4 cut(s)	112, 580, 1466, 1555
Tsp45I	GTSAC	3 cut(s)	2098, 2470, 2908
TspDTI	ATGAA	8 cut(s)	111, 768, 1698, 1798, 2100, 2266, 2441, 2679
TspGWI	ACGGA	2 cut(s)	125, 194
TspRI	CASTG	5 cut(s)	62, 999, 1678, 1698, 1726
Van91I	CCANNNNNTGG	2 cut(s)	1208, 1338
VpaK11BI	GGWCC	4 cut(s)	613, 1279, 1813, 2282
XagI	CCTNNNNNAGG	1 cut(s)	1734
XapI	RAATTY	6 cut(s)	27, 306, 1360, 1411, 2508, 2650
XbaI	TCTAGA	2 cut(s)	856, 2827
XceI	RCATGY	3 cut(s)	367, 398, 1901
XcmI	CCANNNNNNNNNTGG	1 cut(s)	757
XhoI	CTCGAG	1 cut(s)	1921
XmiI	GTMKAC	1 cut(s)	1964
Zsp2I	ATGCAT	1 cut(s)	2462

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.