Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0014382

Physical Location & Seq

Genomic Coordinates Reverse (-)

40596103 .. 40602469

Gene Structure 6367 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1325600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2937 bp

ATGAGTTCTTCTCCTCACGCGCTACTCCATCTCCAACCCCGACCTCCTCCGCAGCCCCCCTTATGCTTCGGCTCGATTCCATTTCCTTCGCAATCACTCACCCAATTCAAGAAATTCAGAAAAAGCAACTTTTCTCTGAAGTTGTACAATAACGGCTCCAAAAGCGGCGTTTTCGGTAGAACTAGCGGCGTAATCAGAAGCTCGGTGGCCGAGGAATCGAATGGTGCGGCGGAGCAGAAGCCGAAGCAGCAAGCCGTGGTGAAGAGGCCCTACCCCTTCCACGAAATCGAGCTCAAATGGCAGTGTTATTGGGAGGAAAACCGGACGTTTCGAACCCCCGATGAAATTGATACCTCCAAGCCCAAATACTATGTTCTTGACATGTTCCCGTATCCCAGCGGTGCTGGGTTACATGTTGGCCATCCACTTGGATATACTGCCACAGACATTCTTGCTAGGTTCAAACGTATGCAGGGTTACAATGTGTTACACCCAATGGGATGGGATGCATTCGGATTGCCGGCGGAGCAATATGCAATAGAGACAGGAACCCACCCGAAGATCACAACCTTGAAGAACATTGATCGCTTTCGCTCCCAGCTTAAATCGTTAGGATTCTCATATGACTGGGATCGCGAAATTTCTACAACAGAACCAGAATATTATAGATGGACCCAATGGATCTTTCTTCAGCTATTAAAGAGAGGATTGGCGTATCAGGCTGAAGTGCCAGTCAATTGGTGCCCTGCTCTTGGTACTGTCTTGGCAAATGAAGAGGTGATAGATGGTTTGAGTGAGCGTGGGGGTCATCCAGTTATAAGAAAGCCAATGAAGCAATGGATGCTCAAGATCACTGCATATGCTGATCGTCTTCTAGACGATTTAGATGACCTTGACTGGCCCGAAAGCATAAAAGAAATGCAAAGAAACTGGATAGGGAGGTCAGAAGGTGCTGAGATGGAATTTCCTATTCTTAGCAGCGATGGACTGGAAAGAGGCACAAAGATTACAATCTATACTACCAGGCCTGACACCATATTTGGAGCAACCTATTTAGTCGTGGCACCCGAGCATCCCTTGTTGTCAACGTTTGTATCAACAGCTCAGAGAGAAACTGTGGAGGAGTACATAGATCTTGCCTCAAGAAAGAGTGACCTTGAGAGGACTGAGCTTCAGAAGGAGAAAACCGGGGTCTTCAGTGGTTGCTACGCTAAAAATCCAGTTAATGGGGAAGCTGTTCCTATATGGGTTGCGGACTATGTCTTGGGGAGTTATGGAACAGGGGCCATTATGGCTGTTCCTGCACATGACACTCGTGATTTTGAGTTTGCATCAAAATTTGACATCCCAATTCGTTGGGTTGTAATGCCGGATGATAAAAATATCAGTGGTTCCAAAAAGGCCTATTCAGGTGAAGGTACTGTTATAAATTCTTCAAATTCAACACTGGGGCTGGACATTAATGACTTATCTAGCAAAGAAGCTGCTTCCAAAGTCATTGACTGGGCTGAGAAAACTACACATGGGAAGAAAAAGGTGAACTTTAAGTTAAGGGATTGGCTTTTTGCTCGGCAACGTTATTGGGGTGAACCTATTCCAGTAGTTTTTTTGGATGATACTGGTGAGACTGTTCCCATACTTGAAACTGAACTGCCCCTTACCCTACCAGAACTGGATGATTTTTCTCCCACTGGGACAGGAGAACCACCACTATCAAAAGCAGTTTCTTGGGTTAAAACTAAGGACCCTTTATCTGGAAAACCAGCTAGACGAGAGACAAGCACGATGCCTCAATGGGCTGGTTCTTGCTGGTACTATTTGAGATTTATGGACCCAAAAAATTCAGAAGAATTGGTGGAAAAGAGGAAAGAAATGTACTGGAACCAAGTTGATGTGTATGTTGGGGGTGCAGAACATGCTGTTCTTCATTTACTTTATTCAAGGTTCTGGCACAAGGTTCTCTATGACATTGGCGCTGTATCCACCAAAGAACCATTCAAGTGTGTCATAAACCAGGGCATTATTCTTGGCGAAGTTCAATATATCGCTTACAAGGACTCTGATGGGAATTTTATCTCTGCCGACTCTGGGACAGCAGTGGAACATCAGCAAGAAATAATACCAGAGGAAAAGGTCATGAAATCGGGGGATTCTTTTGTACTGAAAGACAATCCTGACATTTGTCTGATTGCACGCTCTCATAAAATGAGTAAAAGTAGGGGAAATGTAGTCAATCCCGATGATGTTGTCTCTGAGTACGGGGCGGATTCACTTCGCTTATATGAAATGTTCATGGGGCCACTTAGAGATTCAAAGACCTGGAATACGAGTGGAATTGAAGGTGTCCATCGATTTTTGGGAAGAACATGGAGGCTAGTCGTTGGTTCACCATTGTCTGATGATACATTCAAGGATGGGACAGTAGTGACTGATGGGGAACCTATGTTGGAGCAACTTCGTTCTCTCCATAAGTGCATTGCAAAGGTAACCGAGGAAATAGAAGCAACAAGATTCAACACTGGAATATCTGCGATGATGGAATTCCTCAATGCGGCTTATAAGTGGCAAAACCATCCAAGATTGGTTATTGAAGCATTTGTTTTGTTGCTCTCACCGTATGCACCTCATATGGCCGAGGAGCTTTGGTTTCGCTTGGGACACTCCAAATCTCTGGCATATGAGCCTTTTCCGAAGGCGGATCCTGCTTACTTGAAGGAATCTACTATTGTCCTCCCAGTTCAGATCAACGGCAAGACCAGGGGTACGATCCAGGTTGAAGAGACCTGTTCGGAGGAGGATGCTTTCCAACTAGCAACACAAGATGAAAAACTCTCCAAATATCTGGATGGAAAGTCGATTAAAAAGAGAATTTTTGTCCCCGGCAAGATTCTGAATGTTATCCTGGACCGTGAAAACGTCAAGGCAGCTGCCCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004823 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006429 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032543 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140053 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1 tRNA-synt_1_2

Protein Analysis

979

Amino Acids

110.64

Weight (kDa)

6.04

Isoelectric Point (pI)

39.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	100 - 312	3e-29	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	128 - 260	3.5e-18	tRNA synthetases class I (M)
tRNA-synt_1_2	PF13603	309 - 503	7.9e-63	Leucyl-tRNA synthetase, editing domain
tRNA-synt_1	PF00133	733 - 763	8e-07	tRNA synthetases class I (I, L, M and V)
Anticodon_1	PF08264	819 - 926	1.3e-17	Anticodon-binding domain of tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04350
fragaria_vesca	FvH4_3g01570 FvH4_3g01570
malus_domestica	MD10G1325600.v1.1
prunus_persica	Prupe.4G016400_v2.0.a1 Prupe.4G016400_v2.0.a1
pyrus_communis	pycom10g27530
rosa_chinensis	RchiOBHm_Chr5g0002381
rosa_laevigata	RLG00000031024
rosa_multiflora	Rmu_sc0006137.1_g000003 Rmu_sc0017945.1_g000003
rosa_roxburghii	Rroxscaffold_1G00073490
rosa_rugosa	Rorug04G0395800.1
rosa_samantha	Rh5AG018500 Rh5BG021200 Rh5DG019500
rosa_wichuraiana	Rw5G001740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	818, 1427, 2559
AccB1I	GGYRCC	2 cut(s)	741, 1063
AccB7I	CCANNNNNTGG	1 cut(s)	1226
AccII	CGCG	2 cut(s)	20, 636
AclI	AACGTT	2 cut(s)	1088, 1576
AclWI	GGATC	5 cut(s)	639, 689, 2693, 2706, 2761
AcoI	YGGCCR	3 cut(s)	207, 418, 2631
AcuI	CTGAAG	5 cut(s)	158, 674, 744, 1157, 1180
AfaI	GTAC	9 cut(s)	146, 757, 1127, 1420, 1814, 1877, 2160, 2258, 2764
AfiI	CCNNNNNNNGG	5 cut(s)	752, 1226, 1754, 2088, 2551
AflIII	ACRYGT	2 cut(s)	381, 412
AjnI	CCWGG	6 cut(s)	1022, 2013, 2318, 2756, 2769, 2901
AjuI	GAANNNNNNNTTGG	4 cut(s)	669, 701, 1979, 2011
Alw21I	GWGCWC	1 cut(s)	294
Alw26I	GTCTC	5 cut(s)	536, 1619, 1769, 2254, 2774
AlwI	GGATC	5 cut(s)	639, 689, 2693, 2706, 2761
Ama87I	CYCGRG	1 cut(s)	1067
AoxI	GGCC	9 cut(s)	207, 266, 418, 899, 1025, 1284, 1401, 2297, 2631
ApeKI	GCWGC	6 cut(s)	52, 247, 978, 1484, 2924, 2927
ArsI	GACNNNNNNTTYG	2 cut(s)	1022, 1054
AseI	ATTAAT	1 cut(s)	1461
Asp700I	GAANNNNTTC	2 cut(s)	1236, 1593
AspLEI	GCGC	2 cut(s)	22, 1976
AspS9I	GGNCC	8 cut(s)	267, 672, 900, 1284, 1744, 1831, 2297, 2905
AsuC2I	CCSGG	2 cut(s)	1189, 2880
AsuHPI	GGTGA	9 cut(s)	91, 271, 790, 1424, 1549, 1598, 1634, 2379, 2604
AsuII	TTCGAA	1 cut(s)	331
AvaI	CYCGRG	1 cut(s)	1067
AvaII	GGWCC	4 cut(s)	672, 1744, 1831, 2905
BaeGI	GKGCMC	1 cut(s)	746
BaeI	ACNNNNGTAYC	2 cut(s)	1077, 1110
BalI	TGGCCA	1 cut(s)	420
BamHI	GGATCC	1 cut(s)	2698
BanI	GGYRCC	2 cut(s)	741, 1063
BanII	GRGCYC	1 cut(s)	294
BarI	GAAGNNNNNNTAC	2 cut(s)	254, 286
BauI	CACGAG	1 cut(s)	1314
BbsI	GAAGAC	2 cut(s)	863, 1186
Bbv12I	GWGCWC	1 cut(s)	294
BbvI	GCAGC	5 cut(s)	64, 259, 990, 1471, 2914
BceAI	ACGGC	3 cut(s)	169, 239, 2764
BciT130I	CCWGG	6 cut(s)	1024, 2015, 2320, 2758, 2771, 2903
BciVI	GTATCC	2 cut(s)	402, 1990
BcnI	CCSGG	2 cut(s)	1189, 2880
BcoDI	GTCTC	5 cut(s)	536, 1619, 1769, 2254, 2774
BfaI	CTAG	7 cut(s)	183, 456, 875, 1473, 1767, 2375, 2810
BfoI	RGCGCY	1 cut(s)	1977
BfuI	GTATCC	2 cut(s)	402, 1990
BglI	GCCNNNNNGGC	1 cut(s)	1292
BglII	AGATCT	1 cut(s)	1132
Bme1390I	CCNGG	8 cut(s)	1024, 1189, 2015, 2320, 2758, 2771, 2880, 2903
Bme18I	GGWCC	4 cut(s)	672, 1744, 1831, 2905
BmeT110I	CYCGRG	1 cut(s)	1067
BmgT120I	GGNCC	8 cut(s)	267, 672, 900, 1284, 1744, 1831, 2297, 2905
BmrFI	CCNGG	8 cut(s)	1024, 1189, 2015, 2320, 2758, 2771, 2880, 2903
BmrI	ACTGGG	5 cut(s)	637, 1457, 1513, 1701, 2729
BmsI	GCATC	6 cut(s)	496, 831, 1081, 1340, 1776, 2788
BmuI	ACTGGG	5 cut(s)	637, 1457, 1513, 1701, 2729
BoxI	GACNNNNGTC	1 cut(s)	2181
BpiI	GAAGAC	2 cut(s)	863, 1186
BplI	GAGNNNNNCTC	1 cut(s)	27
Bpu14I	TTCGAA	1 cut(s)	331
BpuEI	CTTGAG	3 cut(s)	830, 1126, 1178
BpuMI	CCSGG	2 cut(s)	1189, 2880
Bsa29I	ATCGAT	1 cut(s)	2350
BsaBI	GATNNNNATC	1 cut(s)	1010
BsaI	GGTCTC	1 cut(s)	2774
BsaJI	CCNNGG	8 cut(s)	210, 255, 1188, 2014, 2490, 2634, 2757, 2878
BsaWI	WCCGGW	1 cut(s)	321
BsaXI	ACNNNNNCTCC	4 cut(s)	15, 45, 2630, 2660
Bsc4I	CCNNNNNNNGG	5 cut(s)	752, 1226, 1754, 2088, 2551
Bse118I	RCCGGY	1 cut(s)	520
Bse3DI	GCAATG	2 cut(s)	842, 2475
Bse8I	GATNNNNATC	1 cut(s)	1010
BseBI	CCWGG	6 cut(s)	1024, 2015, 2320, 2758, 2771, 2903
BseCI	ATCGAT	1 cut(s)	2350
BseDI	CCNNGG	8 cut(s)	210, 255, 1188, 2014, 2490, 2634, 2757, 2878
BseJI	GATNNNNATC	1 cut(s)	1010
BseLI	CCNNNNNNNGG	5 cut(s)	752, 1226, 1754, 2088, 2551
BseMI	GCAATG	2 cut(s)	842, 2475
BseMII	CTCAG	5 cut(s)	945, 1118, 1158, 1500, 2244
BseRI	GAGGAG	4 cut(s)	36, 1136, 2651, 2807
BseSI	GKGCMC	1 cut(s)	746
BseXI	GCAGC	5 cut(s)	64, 259, 990, 1471, 2914
BseYI	CCCAGC	3 cut(s)	395, 404, 597
BsgI	GTGCAG	2 cut(s)	1287, 1929
Bsh1236I	CGCG	2 cut(s)	20, 636
BshFI	GGCC	9 cut(s)	209, 268, 420, 901, 1027, 1286, 1403, 2299, 2633
BshNI	GGYRCC	2 cut(s)	741, 1063
BshVI	ATCGAT	1 cut(s)	2350
BsiHKAI	GWGCWC	1 cut(s)	294
BsiHKCI	CYCGRG	1 cut(s)	1067
BsiSI	CCGG	5 cut(s)	322, 521, 1188, 1370, 2880
BslFI	GGGAC	5 cut(s)	1708, 2104, 2431, 2670, 2861
BslI	CCNNNNNNNGG	5 cut(s)	752, 1226, 1754, 2088, 2551
BsmAI	GTCTC	5 cut(s)	536, 1619, 1769, 2254, 2774
BsmFI	GGGAC	5 cut(s)	1708, 2104, 2431, 2670, 2861
BsmI	GAATGC	1 cut(s)	509
BsnI	GGCC	9 cut(s)	209, 268, 420, 901, 1027, 1286, 1403, 2299, 2633
Bso31I	GGTCTC	1 cut(s)	2774
BsoBI	CYCGRG	1 cut(s)	1067
Bsp119I	TTCGAA	1 cut(s)	331
Bsp1286I	GDGCHC	2 cut(s)	294, 746
Bsp1407I	TGTACA	1 cut(s)	144
Bsp68I	TCGCGA	1 cut(s)	636
BspANI	GGCC	9 cut(s)	209, 268, 420, 901, 1027, 1286, 1403, 2299, 2633
BspCNI	CTCAG	5 cut(s)	946, 1117, 1159, 1501, 2245
BspDI	ATCGAT	1 cut(s)	2350
BspFNI	CGCG	2 cut(s)	20, 636
BspHI	TCATGA	1 cut(s)	2136
BspPI	GGATC	5 cut(s)	639, 689, 2693, 2706, 2761
BspT104I	TTCGAA	1 cut(s)	331
BspT107I	GGYRCC	2 cut(s)	741, 1063
BspTNI	GGTCTC	1 cut(s)	2774
BsrDI	GCAATG	2 cut(s)	842, 2475
BsrFI	RCCGGY	1 cut(s)	520
BsrGI	TGTACA	1 cut(s)	144
BssAI	RCCGGY	1 cut(s)	520
BssECI	CCNNGG	8 cut(s)	210, 255, 1188, 2014, 2490, 2634, 2757, 2878
BssSI	CACGAG	1 cut(s)	1314
Bst2BI	CACGAG	1 cut(s)	1314
Bst2UI	CCWGG	6 cut(s)	1024, 2015, 2320, 2758, 2771, 2903
Bst4CI	ACNGT	7 cut(s)	760, 1117, 1423, 1630, 2422, 2616, 2909
Bst6I	CTCTTC	3 cut(s)	257, 768, 2772
BstAPI	GCANNNNNTGC	2 cut(s)	841, 1916
BstAUI	TGTACA	1 cut(s)	144
BstBI	TTCGAA	1 cut(s)	331
BstC8I	GCNNGC	3 cut(s)	252, 522, 2194
BstDEI	CTNAG	8 cut(s)	954, 974, 1104, 1167, 1509, 1740, 2253, 2303
BstDSI	CCRYGG	1 cut(s)	255
BstEII	GGTNACC	1 cut(s)	2485
BstFNI	CGCG	2 cut(s)	20, 636
BstH2I	RGCGCY	1 cut(s)	1977
BstHHI	GCGC	2 cut(s)	22, 1976
BstMAI	GTCTC	5 cut(s)	536, 1619, 1769, 2254, 2774
BstNI	CCWGG	6 cut(s)	1024, 2015, 2320, 2758, 2771, 2903
BstNSI	RCATGY	3 cut(s)	385, 416, 1919
BstPAI	GACNNNNGTC	1 cut(s)	2181
BstPI	GGTNACC	1 cut(s)	2485
BstSCI	CCNGG	8 cut(s)	1022, 1187, 2013, 2318, 2756, 2769, 2878, 2901
BstSLI	GKGCMC	1 cut(s)	746
BstUI	CGCG	2 cut(s)	20, 636
BstV1I	GCAGC	5 cut(s)	64, 259, 990, 1471, 2914
BstV2I	GAAGAC	2 cut(s)	863, 1186
BstX2I	RGATCY	3 cut(s)	681, 1132, 2698
BstXI	CCANNNNNNTGG	6 cut(s)	428, 501, 738, 1356, 2671, 2842
BstYI	RGATCY	3 cut(s)	681, 1132, 2698
Bsu15I	ATCGAT	1 cut(s)	2350
BsuI	GTATCC	2 cut(s)	402, 1990
BsuRI	GGCC	9 cut(s)	209, 268, 420, 901, 1027, 1286, 1403, 2299, 2633
BsuTUI	ATCGAT	1 cut(s)	2350
BtgI	CCRYGG	1 cut(s)	255
BtgZI	GCGATG	2 cut(s)	996, 2546
BtsI	GCAGTG	3 cut(s)	308, 852, 2103
BtsIMutI	CAGTG	8 cut(s)	308, 852, 1204, 1393, 1445, 1689, 2103, 2517
BtuMI	TCGCGA	1 cut(s)	636
Cac8I	GCNNGC	3 cut(s)	252, 522, 2194
CciI	TCATGA	1 cut(s)	2136
CfoI	GCGC	2 cut(s)	22, 1976
Cfr10I	RCCGGY	1 cut(s)	520
Cfr13I	GGNCC	8 cut(s)	267, 672, 900, 1284, 1744, 1831, 2297, 2905
ClaI	ATCGAT	1 cut(s)	2350
Csp6I	GTAC	9 cut(s)	145, 756, 1126, 1419, 1813, 1876, 2159, 2257, 2763
CviAII	CATG	8 cut(s)	382, 413, 1307, 1523, 1916, 2137, 2293, 2367
CviQI	GTAC	9 cut(s)	145, 756, 1126, 1419, 1813, 1876, 2159, 2257, 2763
DdeI	CTNAG	8 cut(s)	954, 974, 1104, 1167, 1509, 1740, 2253, 2303
EaeI	YGGCCR	3 cut(s)	207, 418, 2631
Eam1104I	CTCTTC	3 cut(s)	257, 768, 2772
EarI	CTCTTC	3 cut(s)	257, 768, 2772
EciI	GGCGGA	4 cut(s)	245, 539, 2279, 2711
Ecl136II	GAGCTC	1 cut(s)	292
Eco147I	AGGCCT	2 cut(s)	1027, 1403
Eco24I	GRGCYC	1 cut(s)	294
Eco31I	GGTCTC	1 cut(s)	2774
Eco47I	GGWCC	4 cut(s)	672, 1744, 1831, 2905
Eco53kI	GAGCTC	1 cut(s)	292
Eco57I	CTGAAG	5 cut(s)	158, 674, 744, 1157, 1180
Eco88I	CYCGRG	1 cut(s)	1067
Eco91I	GGTNACC	1 cut(s)	2485
EcoICRI	GAGCTC	1 cut(s)	292
EcoO109I	RGGNCCY	2 cut(s)	267, 1744
EcoO65I	GGTNACC	1 cut(s)	2485
EcoRI	GAATTC	1 cut(s)	2540
EcoRII	CCWGG	6 cut(s)	1022, 2013, 2318, 2756, 2769, 2901
EcoT22I	ATGCAT	1 cut(s)	511
EcoT38I	GRGCYC	1 cut(s)	294
FaeI	CATG	8 cut(s)	385, 416, 1310, 1526, 1919, 2140, 2296, 2370
FalI	AAGNNNNNCTT	2 cut(s)	1526, 1558
FaqI	GGGAC	5 cut(s)	1708, 2104, 2431, 2670, 2861
FatI	CATG	8 cut(s)	381, 412, 1306, 1522, 1915, 2136, 2292, 2366
FauNDI	CATATG	4 cut(s)	622, 859, 2628, 2677
FriOI	GRGCYC	1 cut(s)	294
FspBI	CTAG	7 cut(s)	183, 456, 875, 1473, 1767, 2375, 2810
GlaI	GCGC	2 cut(s)	21, 1975
GsaI	CCCAGC	3 cut(s)	399, 408, 601
HaeII	RGCGCY	1 cut(s)	1977
HaeIII	GGCC	9 cut(s)	209, 268, 420, 901, 1027, 1286, 1403, 2299, 2633
HapII	CCGG	5 cut(s)	322, 521, 1188, 1370, 2880
HhaI	GCGC	2 cut(s)	22, 1976
Hin1II	CATG	8 cut(s)	385, 416, 1310, 1526, 1919, 2140, 2296, 2370
Hin6I	GCGC	2 cut(s)	20, 1974
HinP1I	GCGC	2 cut(s)	20, 1974
HincII	GTYRAC	1 cut(s)	1086
HindII	GTYRAC	1 cut(s)	1086
HpaII	CCGG	5 cut(s)	322, 521, 1188, 1370, 2880
HphI	GGTGA	9 cut(s)	91, 271, 790, 1424, 1549, 1598, 1634, 2379, 2604
Hpy166II	GTNNAC	4 cut(s)	1086, 1540, 1589, 2387
Hpy8I	GTNNAC	4 cut(s)	1086, 1540, 1589, 2387
HpyAV	CCTTC	8 cut(s)	96, 286, 941, 1171, 1409, 2333, 2686, 2707
HpyCH4III	ACNGT	7 cut(s)	760, 1117, 1423, 1630, 2422, 2616, 2909
HpyCH4IV	ACGT	5 cut(s)	326, 466, 1088, 1576, 2916
HpyF3I	CTNAG	8 cut(s)	954, 974, 1104, 1167, 1509, 1740, 2253, 2303
HpySE526I	ACGT	5 cut(s)	326, 466, 1088, 1576, 2916
Hsp92II	CATG	8 cut(s)	385, 416, 1310, 1526, 1919, 2140, 2296, 2370
HspAI	GCGC	2 cut(s)	20, 1974
KroI	GCCGGC	1 cut(s)	520
KroNI	GCCGGC	1 cut(s)	522
LmnI	GCTCC	7 cut(s)	161, 232, 526, 599, 1043, 2449, 2638
Lsp1109I	GCAGC	5 cut(s)	64, 259, 990, 1471, 2914
LweI	GCATC	6 cut(s)	496, 831, 1081, 1340, 1776, 2788
MaeI	CTAG	7 cut(s)	183, 456, 875, 1473, 1767, 2375, 2810
MaeII	ACGT	5 cut(s)	326, 466, 1088, 1576, 2916
MaeIII	GTNAC	6 cut(s)	408, 476, 486, 1151, 2425, 2485
MfeI	CAATTG	1 cut(s)	736
MflI	RGATCY	3 cut(s)	681, 1132, 2698
MhlI	GDGCHC	2 cut(s)	294, 746
MlsI	TGGCCA	1 cut(s)	420
MluNI	TGGCCA	1 cut(s)	420
MlyI	GAGTC	2 cut(s)	2051, 2078
MmeI	TCCRAC	3 cut(s)	58, 2427, 2830
Mox20I	TGGCCA	1 cut(s)	420
Mph1103I	ATGCAT	1 cut(s)	511
MroNI	GCCGGC	1 cut(s)	520
MroXI	GAANNNNTTC	2 cut(s)	1236, 1593
MscI	TGGCCA	1 cut(s)	420
MseI	TTAA	8 cut(s)	603, 698, 1224, 1461, 1545, 1550, 1734, 2859
MslI	CAYNNNNRTG	1 cut(s)	1999
Msp20I	TGGCCA	1 cut(s)	420
MspA1I	CMGCKG	2 cut(s)	399, 2927
MspI	CCGG	5 cut(s)	322, 521, 1188, 1370, 2880
MspR9I	CCNGG	8 cut(s)	1024, 1189, 2015, 2320, 2758, 2771, 2880, 2903
MunI	CAATTG	1 cut(s)	736
Mva1269I	GAATGC	1 cut(s)	509
MvaI	CCWGG	6 cut(s)	1024, 2015, 2320, 2758, 2771, 2903
MvnI	CGCG	2 cut(s)	20, 636
NaeI	GCCGGC	1 cut(s)	522
NciI	CCSGG	2 cut(s)	1189, 2880
NdeI	CATATG	4 cut(s)	622, 859, 2628, 2677
NgoMIV	GCCGGC	1 cut(s)	520
NlaIII	CATG	8 cut(s)	385, 416, 1310, 1526, 1919, 2140, 2296, 2370
NmeAIII	GCCGAG	3 cut(s)	235, 1549, 2659
NmuCI	GTSAC	2 cut(s)	1151, 2425
NruI	TCGCGA	1 cut(s)	636
NsiI	ATGCAT	1 cut(s)	511
NspI	RCATGY	3 cut(s)	385, 416, 1919
NspV	TTCGAA	1 cut(s)	331
PagI	TCATGA	1 cut(s)	2136
PceI	AGGCCT	2 cut(s)	1027, 1403
PciI	ACATGT	2 cut(s)	381, 412
PcsI	WCGNNNNNNNCGW	1 cut(s)	1065
PctI	GAATGC	1 cut(s)	509
PdiI	GCCGGC	1 cut(s)	522
PdmI	GAANNNNTTC	2 cut(s)	1236, 1593
PfeI	GAWTC	9 cut(s)	76, 215, 615, 2150, 2267, 2309, 2511, 2717, 2887
PflFI	GACNNNGTC	1 cut(s)	1259
PflMI	CCANNNNNTGG	1 cut(s)	1226
PfoI	TCCNGGA	1 cut(s)	2901
PleI	GAGTC	2 cut(s)	2051, 2078
PpsI	GAGTC	2 cut(s)	2051, 2078
PpuMI	RGGWCCY	1 cut(s)	1744
PscI	ACATGT	2 cut(s)	381, 412
PshAI	GACNNNNGTC	1 cut(s)	2181
PshBI	ATTAAT	1 cut(s)	1461
PsiI	TTATAA	3 cut(s)	818, 1427, 2559
Psp124BI	GAGCTC	1 cut(s)	294
Psp1406I	AACGTT	2 cut(s)	1088, 1576
Psp5II	RGGWCCY	1 cut(s)	1744
Psp6I	CCWGG	6 cut(s)	1022, 2013, 2318, 2756, 2769, 2901
PspEI	GGTNACC	1 cut(s)	2485
PspFI	CCCAGC	3 cut(s)	395, 404, 597
PspGI	CCWGG	6 cut(s)	1022, 2013, 2318, 2756, 2769, 2901
PspPI	GGNCC	8 cut(s)	267, 672, 900, 1284, 1744, 1831, 2297, 2905
PspPPI	RGGWCCY	1 cut(s)	1744
PsuI	RGATCY	3 cut(s)	681, 1132, 2698
PsyI	GACNNNGTC	1 cut(s)	1259
PvuII	CAGCTG	1 cut(s)	2927
RruI	TCGCGA	1 cut(s)	636
RsaI	GTAC	9 cut(s)	146, 757, 1127, 1420, 1814, 1877, 2160, 2258, 2764
RsaNI	GTAC	9 cut(s)	145, 756, 1126, 1419, 1813, 1876, 2159, 2257, 2763
RseI	CAYNNNNRTG	1 cut(s)	1999
SacI	GAGCTC	1 cut(s)	294
SaqAI	TTAA	8 cut(s)	603, 698, 1224, 1461, 1545, 1550, 1734, 2859
Sau96I	GGNCC	8 cut(s)	267, 672, 900, 1284, 1744, 1831, 2297, 2905
SchI	GAGTC	2 cut(s)	2051, 2078
ScrFI	CCNGG	8 cut(s)	1024, 1189, 2015, 2320, 2758, 2771, 2880, 2903
SduI	GDGCHC	2 cut(s)	294, 746
SfaNI	GCATC	6 cut(s)	496, 831, 1081, 1340, 1776, 2788
SfuI	TTCGAA	1 cut(s)	331
SinI	GGWCC	4 cut(s)	672, 1744, 1831, 2905
SmiMI	CAYNNNNRTG	1 cut(s)	1999
SmlI	CTYRAG	3 cut(s)	845, 1141, 1157
SmoI	CTYRAG	3 cut(s)	845, 1141, 1157
SseBI	AGGCCT	2 cut(s)	1027, 1403
SspI	AATATT	1 cut(s)	662
SspMI	CTAG	7 cut(s)	183, 456, 875, 1473, 1767, 2375, 2810
SstI	GAGCTC	1 cut(s)	294
StuI	AGGCCT	2 cut(s)	1027, 1403
StyD4I	CCNGG	8 cut(s)	1022, 1187, 2013, 2318, 2756, 2769, 2878, 2901
TaaI	ACNGT	7 cut(s)	760, 1117, 1423, 1630, 2422, 2616, 2909
TaiI	ACGT	5 cut(s)	329, 469, 1091, 1579, 2919
TaqI	TCGA	6 cut(s)	74, 218, 288, 331, 2350, 2855
TatI	WGTACW	4 cut(s)	144, 1125, 1875, 2158
TauI	GCSGC	4 cut(s)	168, 189, 230, 2555
TfiI	GAWTC	9 cut(s)	76, 215, 615, 2150, 2267, 2309, 2511, 2717, 2887
Tru1I	TTAA	8 cut(s)	603, 698, 1224, 1461, 1545, 1550, 1734, 2859
Tru9I	TTAA	8 cut(s)	603, 698, 1224, 1461, 1545, 1550, 1734, 2859
TscAI	CASTG	8 cut(s)	308, 859, 1204, 1393, 1452, 1696, 2103, 2524
TseFI	GTSAC	2 cut(s)	1151, 2425
TseI	GCWGC	6 cut(s)	52, 247, 978, 1484, 2924, 2927
Tsp45I	GTSAC	2 cut(s)	1151, 2425
TspDTI	ATGAA	8 cut(s)	357, 786, 845, 1916, 2153, 2281, 2298, 2838
TspRI	CASTG	8 cut(s)	308, 859, 1204, 1393, 1452, 1696, 2103, 2524
Tth111I	GACNNNGTC	1 cut(s)	1259
Van91I	CCANNNNNTGG	1 cut(s)	1226
VpaK11BI	GGWCC	4 cut(s)	672, 1744, 1831, 2905
VspI	ATTAAT	1 cut(s)	1461
XbaI	TCTAGA	1 cut(s)	874
XceI	RCATGY	3 cut(s)	385, 416, 1919
XcmI	CCANNNNNNNNNTGG	1 cut(s)	834
XmnI	GAANNNNTTC	2 cut(s)	1236, 1593
XspI	CTAG	7 cut(s)	183, 456, 875, 1473, 1767, 2375, 2810
Zsp2I	ATGCAT	1 cut(s)	511

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.