Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0014382

Physical Location & Seq

Genomic Coordinates Forward (+)

1328164 .. 1335476

Gene Structure 7313 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG018500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2598 bp

ATGGAAGGATTTGGATTTGATAACAAATTTAAGTCAGTTAAGCGTAACGGAAACATGAGTGGAGCGGGGTTACATGTTGGCCATCCGCTTGGATATACTGCGACAGATATTCTTGCTAGGCTCAAACGTATGCAGGGTTACAATGTATTACACCCAATGGGATGGGATGCATTTGGATTGCCAGCGGAGCAATATGCGATCGAGACAGGAACCCACCCAAAGATCACAACCTTGAAGAACATTGACCGCTTTCGTTCCCAGCTTAAATCGTTAGGTTTCTCATATGACTGGGATCGTGAAATTTCTACAACAGAACCAGAATATTATAAATGGACCCAATGGATCTTTCTTCAGCTATTAAAGAGAGGCTTGGCATATCAGGCTGAAGTGCCAGTCAATTGGTGCCCTGCTCTTGGTACTGTGTTGGCAAATGAAGAAGTGATAGATGGTCTGAGCGAGCGTGGGGGTCATCCAGTTATAAGAAAGCCAATGAAGCAATGGATGCTCAAGATTACGGCATATGCTGATCGCCTTCTTGAAGATTTAGATGACCTTGACTGGCCTGAGAGTATCAAAGAGATGCAAAGAAACTGGATAGGGAAGTCAGAAGGCGCTGAGATGGAATTTTCTGTTCTTAGTAGTGATGGACAAGAGAGAGACATAAAGATTACAATATATACTACCAGACCGGACACCATCTTTGGAGCAACATATTTAGTCATCGCACCAGAGCATCCCTTGCTGTCATCATTAGTATCAACAGCCCAGAGAGAAAGTGTGGAGGAGTACGTAGATGTTGCCTCAAGAAAGAGTGACCTTGAGAGGACTGAGCTTCAAAAGGAAAAGACCGGGGTGTTCAGTGGTTGCTATGCTAAAAATCCAGTTAATGGAGAAGCTATCCCTATCTGGGTTGCGGACTATGTATTGGCCAGTTATGGAACGGGAGCAATAATGGCTGTACCTGCACACGATGCTCGTGACTATGAATTTGCTTCAAAATATGATATCCCAATCCGTTGGGTTGTGATGCCGCATGATAAAAAGCTCAGTGGTTCCGGAAAGGCCTATTCAGGTGAAGGCAATGTTATAAATTCTTCAAACACAACATTGGGGCTGGACATTAATAGCTTATCTAGCAAAGAAGCTGCTTTCAAAGTCATTGAATGGGCCGAGAAAACTGGAAATGGGAAGAAAAAGGTGAACTATAAGTTGAGGGATTGGCTTTTTGCTAGGCAACGCTATTGGGGGGAACCTATTCCAGTAGTTTTTTCAGATGATGGTGATGAGGGTGTTCCCATTCATGAAACCGAACTGCCCCTCACCCTTCCAGAATTGGATGATTTTTCTCCCACTGGAACAGGAGAACCACCCCTAGCAAAAGCAGTTTCTTGGGTCAATACTAAGGATCCTTTGTCTGGAAAACCTGCCAGACGAGAGACAAGCACCATGCCCCAGTGGGCTGGTTCTTGCTGGTACTATTTGAGATTTATGGACCCAAAAAATTCAAAAGAATTGGTGGCCAAGACAAAAGAAATGTATTGGAGCCCAGTTGATGTGTATGTTGGTGGTGCTGAACATGCTGTTCTCCATTTACTTTATGCAAGATTCTGGCACAAGGTTCTCTATGACATTGGTGTCGTCTCCACCAAAGAACCCTTCAAGTGTGTCATAAATCAGGGCATTATTCTTGGAGAAGTTCAATATATCGCATACAAGGACTCAGATGGGAACTTTGTCTCTGCAGACTCTGATACATCAGCTGAGCTTCAACAAGAAATAATACCGGAGGAAAAGGTCATTAAATCCGGGGATTCTTTTGTACTAAAAGACAATCCTGAGATCTCTCTTATTGCACGTTCTCATAAAATGAGTAAGAGTAGGGGAAATGTTGTCAATCCTGATGATGTTGTTTCTGAGTACGGGGCCGATTCACTTCGCTTATATGAAATGTTCATGGGGCCGTTAAGAGACTCAAAACAATGGAATACGAGTGGTATTGAAGGTGTTCATCGGTTTTTGGGAAGAGCCTGGAGGCTAGTTGTTGGTTCAATGTTATCTGATGGTACACTCAAAGAGGGAACAGTAGTGACTGATGAGGATCCCACTTCGGAGCAACTTCGTTCTCTACATAAGTGCATTGCCAAGGTAACAGAGGAAATAGAAGCGACGAGATTTAATACTGGAATTTCTGCAATGATGGAGTTCATCAATGTGGCATTTAAGTGGAAAAAACATCCGAGAGTGATTATTGAAGCATTTGTTTTATTGCTCGCACCATATGCACCTCATATGGCTGAGGAGCTTTGGTTTCGCTTGGGACACTCGAAATCATTGGCATACGAGGCTTTTCCTAAGGTCAATCCTGCTTACTTGGAGGAATCTACTATTTTGCTACCGGTTCAGATCAATGGCAAGACGAGGGGTACAATCCAGGTTGAAAAGACCTGTTCGGAGGAGGATGCATTCCTACTGGCATCGAAAGATGGAAAGCTCTCCAAATATCTTGATGGGGTGTCAATTAAAAAGAGGATTTTCGTCCCTGGAAAAATCCTGAATGTCATCTTGGACCGTCAAAACGTCAAGGCAGCTGTGCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004823 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006429 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032543 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140053 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1 tRNA-synt_1_2

Protein Analysis

865

Amino Acids

97.24

Weight (kDa)

5.94

Isoelectric Point (pI)

35.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	23 - 200	3.1e-24	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	24 - 147	1.5e-16	tRNA synthetases class I (M)
tRNA-synt_1_2	PF13603	196 - 388	3.3e-63	Leucyl-tRNA synthetase, editing domain
tRNA-synt_1	PF00133	620 - 650	7.5e-07	tRNA synthetases class I (I, L, M and V)
Anticodon_1	PF08264	706 - 814	1.4e-17	Anticodon-binding domain of tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04350
fragaria_vesca	FvH4_3g01570 FvH4_3g01570
malus_domestica	MD10G1325600.v1.1
prunus_persica	Prupe.4G016400_v2.0.a1 Prupe.4G016400_v2.0.a1
pyrus_communis	pycom10g27530
rosa_chinensis	RchiOBHm_Chr5g0002381
rosa_laevigata	RLG00000031024
rosa_multiflora	Rmu_sc0006137.1_g000003 Rmu_sc0017945.1_g000003
rosa_roxburghii	Rroxscaffold_1G00073490
rosa_rugosa	Rorug04G0395800.1
rosa_samantha	Rh5AG018500 Rh5BG021200 Rh5DG019500
rosa_wichuraiana	Rw5G001740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	327, 479, 1088
AasI	GACNNNNNNGTC	1 cut(s)	1634
Acc36I	ACCTGC	2 cut(s)	970, 1432
AccB1I	GGYRCC	1 cut(s)	402
AccB7I	CCANNNNNTGG	1 cut(s)	887
AccBSI	CCGCTC	1 cut(s)	65
AccIII	TCCGGA	1 cut(s)	1055
AciI	CCGC	6 cut(s)	65, 86, 185, 247, 914, 1031
AclWI	GGATC	6 cut(s)	300, 350, 1400, 1413, 2093, 2106
AcoI	YGGCCR	3 cut(s)	79, 927, 1518
AcsI	RAATTY	7 cut(s)	26, 300, 623, 986, 1090, 1501, 2184
AcuI	CTGAAG	2 cut(s)	335, 405
AfaI	GTAC	8 cut(s)	418, 788, 960, 1475, 1821, 1919, 2065, 2425
AfiI	CCNNNNNNNGG	4 cut(s)	413, 887, 907, 1415
AflIII	ACRYGT	1 cut(s)	73
AgeI	ACCGGT	1 cut(s)	2395
AjnI	CCWGG	3 cut(s)	2027, 2430, 2539
AjuI	GAANNNNNNNTTGG	6 cut(s)	330, 362, 1640, 1672, 2546, 2578
Alw26I	GTCTC	6 cut(s)	197, 651, 1430, 1645, 1741, 1962
AlwI	GGATC	6 cut(s)	300, 350, 1400, 1413, 2093, 2106
AlwNI	CAGNNNCTG	1 cut(s)	1748
Aor13HI	TCCGGA	1 cut(s)	1055
AoxI	GGCC	8 cut(s)	79, 560, 927, 1062, 1167, 1518, 1923, 1958
ApeKI	GCWGC	2 cut(s)	1145, 2585
ApoI	RAATTY	7 cut(s)	26, 300, 623, 986, 1090, 1501, 2184
ArsI	GACNNNNNNTTYG	4 cut(s)	683, 715, 971, 1003
AseI	ATTAAT	1 cut(s)	1122
AsiGI	ACCGGT	1 cut(s)	2395
Asp700I	GAANNNNTTC	2 cut(s)	1254, 2004
AspLEI	GCGC	1 cut(s)	614
AspS9I	GGNCC	6 cut(s)	333, 1167, 1492, 1923, 1958, 2566
AsuC2I	CCSGG	2 cut(s)	850, 1807
AsuHPI	GGTGA	4 cut(s)	1085, 1210, 1292, 1312
AvaII	GGWCC	3 cut(s)	333, 1492, 2566
AxyI	CCTNAGG	1 cut(s)	2352
BaeGI	GKGCMC	1 cut(s)	407
BalI	TGGCCA	3 cut(s)	81, 929, 1520
BamHI	GGATCC	2 cut(s)	1405, 2098
BanI	GGYRCC	1 cut(s)	402
BanII	GRGCYC	1 cut(s)	1547
BauI	CACGAG	1 cut(s)	975
BbvCI	CCTCAGC	1 cut(s)	2295
BbvI	GCAGC	1 cut(s)	1132
BceAI	ACGGC	2 cut(s)	531, 1945
BciT130I	CCWGG	3 cut(s)	2029, 2432, 2541
BcnI	CCSGG	2 cut(s)	850, 1807
BcoDI	GTCTC	6 cut(s)	197, 651, 1430, 1645, 1741, 1962
BfaI	CTAG	5 cut(s)	117, 1134, 1230, 1373, 2036
BfmI	CTRYAG	1 cut(s)	1740
BfoI	RGCGCY	1 cut(s)	615
BfuAI	ACCTGC	2 cut(s)	970, 1432
BglII	AGATCT	1 cut(s)	1839
BisI	GCNGC	3 cut(s)	1031, 1146, 2586
BlpI	GCTNAGC	1 cut(s)	1761
BlsI	GCNGC	3 cut(s)	1032, 1147, 2587
Bme1390I	CCNGG	5 cut(s)	850, 1807, 2029, 2432, 2541
Bme18I	GGWCC	3 cut(s)	333, 1492, 2566
BmgT120I	GGNCC	6 cut(s)	333, 1167, 1492, 1923, 1958, 2566
BmrFI	CCNGG	5 cut(s)	850, 1807, 2029, 2432, 2541
BmrI	ACTGGG	3 cut(s)	298, 1447, 1541
BmsI	GCATC	8 cut(s)	157, 492, 570, 742, 961, 1017, 2449, 2483
BmuI	ACTGGG	3 cut(s)	298, 1447, 1541
BpmI	CTGGAG	1 cut(s)	2050
Bpu10I	CCTNAGC	1 cut(s)	2295
Bpu1102I	GCTNAGC	1 cut(s)	1761
BpuEI	CTTGAG	3 cut(s)	491, 787, 839
BpuMI	CCSGG	2 cut(s)	850, 1807
BsaAI	YACGTR	1 cut(s)	790
BsaBI	GATNNNNATC	1 cut(s)	2097
BsaJI	CCNNGG	4 cut(s)	849, 1806, 2142, 2539
BsaWI	WCCGGW	4 cut(s)	688, 1055, 1783, 2395
BsaXI	ACNNNNNCTCC	2 cut(s)	2291, 2321
Bsc4I	CCNNNNNNNGG	4 cut(s)	413, 887, 907, 1415
Bse118I	RCCGGY	1 cut(s)	2395
Bse21I	CCTNAGG	1 cut(s)	2352
Bse3DI	GCAATG	4 cut(s)	503, 1087, 2136, 2199
Bse8I	GATNNNNATC	1 cut(s)	2097
BseAI	TCCGGA	1 cut(s)	1055
BseBI	CCWGG	3 cut(s)	2029, 2432, 2541
BseDI	CCNNGG	4 cut(s)	849, 1806, 2142, 2539
BseGI	GGATG	9 cut(s)	82, 167, 172, 469, 507, 733, 1342, 2233, 2464
BseJI	GATNNNNATC	1 cut(s)	2097
BseLI	CCNNNNNNNGG	4 cut(s)	413, 887, 907, 1415
BseMI	GCAATG	4 cut(s)	503, 1087, 2136, 2199
BseRI	GAGGAG	3 cut(s)	797, 2312, 2468
BseSI	GKGCMC	1 cut(s)	407
BseXI	GCAGC	1 cut(s)	1132
BseYI	CCCAGC	1 cut(s)	258
BsgI	GTGCAG	1 cut(s)	948
Bsh1285I	CGRYCG	1 cut(s)	201
BshFI	GGCC	8 cut(s)	81, 562, 929, 1064, 1169, 1520, 1925, 1960
BshNI	GGYRCC	1 cut(s)	402
BshTI	ACCGGT	1 cut(s)	2395
BsiEI	CGRYCG	1 cut(s)	201
BsiSI	CCGG	6 cut(s)	689, 849, 1056, 1784, 1806, 2396
BslFI	GGGAC	2 cut(s)	2331, 2522
BslI	CCNNNNNNNGG	4 cut(s)	413, 887, 907, 1415
BsmAI	GTCTC	6 cut(s)	197, 651, 1430, 1645, 1741, 1962
BsmBI	CGTCTC	1 cut(s)	1645
BsmFI	GGGAC	2 cut(s)	2331, 2522
BsmI	GAATGC	1 cut(s)	2462
BsnI	GGCC	8 cut(s)	81, 562, 929, 1064, 1169, 1520, 1925, 1960
Bsp1286I	GDGCHC	2 cut(s)	407, 1547
Bsp13I	TCCGGA	1 cut(s)	1055
Bsp143I	GATC	9 cut(s)	198, 222, 292, 342, 526, 1405, 1839, 2098, 2403
Bsp1720I	GCTNAGC	1 cut(s)	1761
BspACI	CCGC	6 cut(s)	65, 86, 185, 247, 914, 1031
BspANI	GGCC	8 cut(s)	81, 562, 929, 1064, 1169, 1520, 1925, 1960
BspEI	TCCGGA	1 cut(s)	1055
BspHI	TCATGA	1 cut(s)	1300
BspMAI	CTGCAG	1 cut(s)	1744
BspMI	ACCTGC	2 cut(s)	970, 1432
BspPI	GGATC	6 cut(s)	300, 350, 1400, 1413, 2093, 2106
BspQI	GCTCTTC	1 cut(s)	2017
BspT107I	GGYRCC	1 cut(s)	402
BsrBI	CCGCTC	1 cut(s)	65
BsrDI	GCAATG	4 cut(s)	503, 1087, 2136, 2199
BsrFI	RCCGGY	1 cut(s)	2395
BssAI	RCCGGY	1 cut(s)	2395
BssECI	CCNNGG	4 cut(s)	849, 1806, 2142, 2539
BssMI	GATC	9 cut(s)	198, 222, 292, 342, 526, 1405, 1839, 2098, 2403
BssSI	CACGAG	1 cut(s)	975
BssT1I	CCWWGG	1 cut(s)	2142
Bst2BI	CACGAG	1 cut(s)	975
Bst2UI	CCWGG	3 cut(s)	2029, 2432, 2541
Bst4CI	ACNGT	3 cut(s)	421, 2083, 2570
Bst6I	CTCTTC	1 cut(s)	2017
BstAPI	GCANNNNNTGC	4 cut(s)	502, 739, 971, 2279
BstBAI	YACGTR	1 cut(s)	790
BstC8I	GCNNGC	3 cut(s)	183, 458, 2271
BstF5I	GGATG	9 cut(s)	82, 167, 172, 469, 507, 733, 1342, 2233, 2464
BstH2I	RGCGCY	1 cut(s)	615
BstHHI	GCGC	1 cut(s)	614
BstKTI	GATC	9 cut(s)	201, 225, 295, 345, 529, 1408, 1842, 2101, 2406
BstMAI	GTCTC	6 cut(s)	197, 651, 1430, 1645, 1741, 1962
BstMBI	GATC	9 cut(s)	198, 222, 292, 342, 526, 1405, 1839, 2098, 2403
BstMCI	CGRYCG	1 cut(s)	201
BstNI	CCWGG	3 cut(s)	2029, 2432, 2541
BstNSI	RCATGY	2 cut(s)	77, 1580
BstSCI	CCNGG	5 cut(s)	848, 1805, 2027, 2430, 2539
BstSFI	CTRYAG	1 cut(s)	1740
BstSLI	GKGCMC	1 cut(s)	407
BstSNI	TACGTA	1 cut(s)	790
BstV1I	GCAGC	1 cut(s)	1132
BstX2I	RGATCY	4 cut(s)	342, 1405, 1839, 2098
BstXI	CCANNNNNNTGG	5 cut(s)	89, 162, 399, 1017, 1460
BstYI	RGATCY	4 cut(s)	342, 1405, 1839, 2098
Bsu36I	CCTNAGG	1 cut(s)	2352
BsuRI	GGCC	8 cut(s)	81, 562, 929, 1064, 1169, 1520, 1925, 1960
BtgZI	GCGATG	1 cut(s)	706
BtsCI	GGATG	9 cut(s)	82, 167, 172, 469, 507, 733, 1342, 2233, 2464
BtsIMutI	CAGTG	4 cut(s)	865, 1054, 1350, 1460
BveI	ACCTGC	2 cut(s)	970, 1432
Cac8I	GCNNGC	3 cut(s)	183, 458, 2271
CaiI	CAGNNNCTG	1 cut(s)	1748
CciI	TCATGA	1 cut(s)	1300
CfoI	GCGC	1 cut(s)	614
Cfr10I	RCCGGY	1 cut(s)	2395
Cfr13I	GGNCC	6 cut(s)	333, 1167, 1492, 1923, 1958, 2566
Csp6I	GTAC	8 cut(s)	417, 787, 959, 1474, 1820, 1918, 2064, 2424
CspAI	ACCGGT	1 cut(s)	2395
CviAII	CATG	7 cut(s)	55, 74, 1034, 1301, 1447, 1577, 1954
CviQI	GTAC	8 cut(s)	417, 787, 959, 1474, 1820, 1918, 2064, 2424
DpnI	GATC	9 cut(s)	200, 224, 294, 344, 528, 1407, 1841, 2100, 2405
DpnII	GATC	9 cut(s)	198, 222, 292, 342, 526, 1405, 1839, 2098, 2403
DrdI	GACNNNNNNGTC	1 cut(s)	1634
DseDI	GACNNNNNNGTC	1 cut(s)	1634
EaeI	YGGCCR	3 cut(s)	79, 927, 1518
Eam1104I	CTCTTC	1 cut(s)	2017
EarI	CTCTTC	1 cut(s)	2017
Eco105I	TACGTA	1 cut(s)	790
Eco130I	CCWWGG	1 cut(s)	2142
Eco147I	AGGCCT	1 cut(s)	1064
Eco24I	GRGCYC	1 cut(s)	1547
Eco32I	GATATC	1 cut(s)	1006
Eco47I	GGWCC	3 cut(s)	333, 1492, 2566
Eco57I	CTGAAG	2 cut(s)	335, 405
Eco81I	CCTNAGG	1 cut(s)	2352
EcoRII	CCWGG	3 cut(s)	2027, 2430, 2539
EcoRV	GATATC	1 cut(s)	1006
EcoT14I	CCWWGG	1 cut(s)	2142
EcoT22I	ATGCAT	2 cut(s)	172, 2464
EcoT38I	GRGCYC	1 cut(s)	1547
ErhI	CCWWGG	1 cut(s)	2142
Esp3I	CGTCTC	1 cut(s)	1645
FaeI	CATG	7 cut(s)	58, 77, 1037, 1304, 1450, 1580, 1957
FalI	AAGNNNNNCTT	2 cut(s)	353, 385
FaqI	GGGAC	2 cut(s)	2331, 2522
FatI	CATG	7 cut(s)	54, 73, 1033, 1300, 1446, 1576, 1953
FauI	CCCGC	1 cut(s)	58
FauNDI	CATATG	4 cut(s)	283, 520, 2278, 2289
Fnu4HI	GCNGC	3 cut(s)	1031, 1146, 2586
FokI	GGATG	9 cut(s)	69, 174, 179, 456, 514, 720, 1349, 2220, 2471
FriOI	GRGCYC	1 cut(s)	1547
Fsp4HI	GCNGC	3 cut(s)	1031, 1146, 2586
FspBI	CTAG	5 cut(s)	117, 1134, 1230, 1373, 2036
GlaI	GCGC	1 cut(s)	613
GluI	GCNGC	3 cut(s)	1031, 1146, 2586
GsaI	CCCAGC	1 cut(s)	262
GsuI	CTGGAG	1 cut(s)	2050
HaeII	RGCGCY	1 cut(s)	615
HaeIII	GGCC	8 cut(s)	81, 562, 929, 1064, 1169, 1520, 1925, 1960
HapII	CCGG	6 cut(s)	689, 849, 1056, 1784, 1806, 2396
HhaI	GCGC	1 cut(s)	614
Hin1II	CATG	7 cut(s)	58, 77, 1037, 1304, 1450, 1580, 1957
Hin6I	GCGC	1 cut(s)	612
HinP1I	GCGC	1 cut(s)	612
HinfI	GANTC	7 cut(s)	1605, 1718, 1745, 1811, 1928, 1970, 2378
HpaII	CCGG	6 cut(s)	689, 849, 1056, 1784, 1806, 2396
HphI	GGTGA	4 cut(s)	1085, 1210, 1292, 1312
Hpy166II	GTNNAC	2 cut(s)	1201, 2066
Hpy8I	GTNNAC	2 cut(s)	1201, 2066
Hpy99I	CGWCG	1 cut(s)	2170
HpyAV	CCTTC	6 cut(s)	542, 602, 1070, 1334, 1666, 1994
HpyCH4III	ACNGT	3 cut(s)	421, 2083, 2570
HpyCH4IV	ACGT	4 cut(s)	127, 789, 1855, 2577
HpySE526I	ACGT	4 cut(s)	127, 789, 1855, 2577
Hsp92II	CATG	7 cut(s)	58, 77, 1037, 1304, 1450, 1580, 1957
HspAI	GCGC	1 cut(s)	612
Kpn2I	TCCGGA	1 cut(s)	1055
Kzo9I	GATC	9 cut(s)	198, 222, 292, 342, 526, 1405, 1839, 2098, 2403
LguI	GCTCTTC	1 cut(s)	2017
LmnI	GCTCC	7 cut(s)	62, 187, 704, 944, 1542, 2110, 2299
Lsp1109I	GCAGC	1 cut(s)	1132
LweI	GCATC	8 cut(s)	157, 492, 570, 742, 961, 1017, 2449, 2483
MaeI	CTAG	5 cut(s)	117, 1134, 1230, 1373, 2036
MaeII	ACGT	4 cut(s)	127, 789, 1855, 2577
MaeIII	GTNAC	7 cut(s)	44, 69, 137, 812, 977, 2086, 2146
MalI	GATC	9 cut(s)	200, 224, 294, 344, 528, 1407, 1841, 2100, 2405
MbiI	CCGCTC	1 cut(s)	65
MboI	GATC	9 cut(s)	198, 222, 292, 342, 526, 1405, 1839, 2098, 2403
MboII	GAAGA	7 cut(s)	247, 341, 446, 551, 1086, 1201, 2034
MfeI	CAATTG	1 cut(s)	397
MflI	RGATCY	4 cut(s)	342, 1405, 1839, 2098
MhlI	GDGCHC	2 cut(s)	407, 1547
MlsI	TGGCCA	3 cut(s)	81, 929, 1520
MluNI	TGGCCA	3 cut(s)	81, 929, 1520
MlyI	GAGTC	3 cut(s)	1712, 1739, 1964
Mox20I	TGGCCA	3 cut(s)	81, 929, 1520
Mph1103I	ATGCAT	2 cut(s)	172, 2464
MroI	TCCGGA	1 cut(s)	1055
MroXI	GAANNNNTTC	2 cut(s)	1254, 2004
MscI	TGGCCA	3 cut(s)	81, 929, 1520
MslI	CAYNNNNRTG	2 cut(s)	2210, 2221
Msp20I	TGGCCA	3 cut(s)	81, 929, 1520
MspA1I	CMGCKG	3 cut(s)	185, 1760, 2588
MspI	CCGG	6 cut(s)	689, 849, 1056, 1784, 1806, 2396
MspR9I	CCNGG	5 cut(s)	850, 1807, 2029, 2432, 2541
MunI	CAATTG	1 cut(s)	397
Mva1269I	GAATGC	1 cut(s)	2462
MvaI	CCWGG	3 cut(s)	2029, 2432, 2541
NciI	CCSGG	2 cut(s)	850, 1807
NdeI	CATATG	4 cut(s)	283, 520, 2278, 2289
NdeII	GATC	9 cut(s)	198, 222, 292, 342, 526, 1405, 1839, 2098, 2403
NlaIII	CATG	7 cut(s)	58, 77, 1037, 1304, 1450, 1580, 1957
NmeAIII	GCCGAG	1 cut(s)	1195
NmuCI	GTSAC	3 cut(s)	812, 977, 2086
NsiI	ATGCAT	2 cut(s)	172, 2464
NspI	RCATGY	2 cut(s)	77, 1580
PagI	TCATGA	1 cut(s)	1300
PceI	AGGCCT	1 cut(s)	1064
PciI	ACATGT	1 cut(s)	73
PciSI	GCTCTTC	1 cut(s)	2017
PctI	GAATGC	1 cut(s)	2462
PdmI	GAANNNNTTC	2 cut(s)	1254, 2004
PfeI	GAWTC	4 cut(s)	1605, 1811, 1928, 2378
PflMI	CCANNNNNTGG	1 cut(s)	887
PinAI	ACCGGT	1 cut(s)	2395
PkrI	GCNGC	3 cut(s)	1032, 1147, 2587
Ple19I	CGATCG	1 cut(s)	201
PleI	GAGTC	3 cut(s)	1712, 1739, 1964
PpsI	GAGTC	3 cut(s)	1712, 1739, 1964
Ppu21I	YACGTR	1 cut(s)	790
PscI	ACATGT	1 cut(s)	73
PshBI	ATTAAT	1 cut(s)	1122
PsiI	TTATAA	3 cut(s)	327, 479, 1088
Psp6I	CCWGG	3 cut(s)	2027, 2430, 2539
PspFI	CCCAGC	1 cut(s)	258
PspGI	CCWGG	3 cut(s)	2027, 2430, 2539
PspPI	GGNCC	6 cut(s)	333, 1167, 1492, 1923, 1958, 2566
PstI	CTGCAG	1 cut(s)	1744
PstNI	CAGNNNCTG	1 cut(s)	1748
PsuI	RGATCY	4 cut(s)	342, 1405, 1839, 2098
PvuI	CGATCG	1 cut(s)	201
PvuII	CAGCTG	2 cut(s)	1760, 2588
RsaI	GTAC	8 cut(s)	418, 788, 960, 1475, 1821, 1919, 2065, 2425
RsaNI	GTAC	8 cut(s)	417, 787, 959, 1474, 1820, 1918, 2064, 2424
RseI	CAYNNNNRTG	2 cut(s)	2210, 2221
SapI	GCTCTTC	1 cut(s)	2017
SatI	GCNGC	3 cut(s)	1031, 1146, 2586
Sau3AI	GATC	9 cut(s)	198, 222, 292, 342, 526, 1405, 1839, 2098, 2403
Sau96I	GGNCC	6 cut(s)	333, 1167, 1492, 1923, 1958, 2566
SchI	GAGTC	3 cut(s)	1712, 1739, 1964
ScrFI	CCNGG	5 cut(s)	850, 1807, 2029, 2432, 2541
SduI	GDGCHC	2 cut(s)	407, 1547
SfaNI	GCATC	8 cut(s)	157, 492, 570, 742, 961, 1017, 2449, 2483
SfcI	CTRYAG	1 cut(s)	1740
SinI	GGWCC	3 cut(s)	333, 1492, 2566
SmiMI	CAYNNNNRTG	2 cut(s)	2210, 2221
SmlI	CTYRAG	3 cut(s)	506, 802, 818
SmoI	CTYRAG	3 cut(s)	506, 802, 818
SnaBI	TACGTA	1 cut(s)	790
SseBI	AGGCCT	1 cut(s)	1064
SsiI	CCGC	6 cut(s)	65, 86, 185, 247, 914, 1031
SspI	AATATT	1 cut(s)	323
SspMI	CTAG	5 cut(s)	117, 1134, 1230, 1373, 2036
StuI	AGGCCT	1 cut(s)	1064
StyD4I	CCNGG	5 cut(s)	848, 1805, 2027, 2430, 2539
StyI	CCWWGG	1 cut(s)	2142
TaaI	ACNGT	3 cut(s)	421, 2083, 2570
TaiI	ACGT	4 cut(s)	130, 792, 1858, 2580
TaqI	TCGA	3 cut(s)	201, 2324, 2477
TatI	WGTACW	1 cut(s)	1819
TauI	GCSGC	1 cut(s)	1033
TfiI	GAWTC	4 cut(s)	1605, 1811, 1928, 2378
TscAI	CASTG	4 cut(s)	865, 1054, 1357, 1460
TseFI	GTSAC	3 cut(s)	812, 977, 2086
TseI	GCWGC	2 cut(s)	1145, 2585
Tsp45I	GTSAC	3 cut(s)	812, 977, 2086
TspDTI	ATGAA	9 cut(s)	447, 506, 999, 1289, 1317, 1942, 1959, 1997, 2194
TspGWI	ACGGA	2 cut(s)	63, 1004
TspRI	CASTG	4 cut(s)	865, 1054, 1357, 1460
Van91I	CCANNNNNTGG	1 cut(s)	887
VpaK11BI	GGWCC	3 cut(s)	333, 1492, 2566
VspI	ATTAAT	1 cut(s)	1122
XapI	RAATTY	7 cut(s)	26, 300, 623, 986, 1090, 1501, 2184
XceI	RCATGY	2 cut(s)	77, 1580
XcmI	CCANNNNNNNNNTGG	1 cut(s)	495
XmnI	GAANNNNTTC	2 cut(s)	1254, 2004
XspI	CTAG	5 cut(s)	117, 1134, 1230, 1373, 2036
Zsp2I	ATGCAT	2 cut(s)	172, 2464

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.