Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0014382

Physical Location & Seq

Genomic Coordinates Forward (+)

1384425 .. 1391611

Gene Structure 7187 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ28376

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2880 bp

ATGAGTTCTACTCACACCCAACTGGGTCTCCAACCCCAACCACGCCTAACCTTCAACTCACTTCTACTCCCCTCACACCCACTCATCACTTTCTCCCCCAGACTCCACAACCGCAGCTTCAAACGCCGCGTTTCCATCACAGCCTCAGCTTCCAATGAACCCAACGACGTCGTAGAGCAGAAGCCGAAGCAGCAATCGGCGGTGAAGAGGGCCTATCCTTTCCACGAAATCGAGCCCAAGTGGCAGCGTTATTGGGAGGAAGATGGAACCTTTCGAACCCCGGATGAAGTTGACGTGTCAAAGCCCAAGTTCTATGTTCTTGACATGTTCCCTTATCCCAGTGGAGCGGGGTTACATGTTGGCCATCCGCTTGGATATACTGCGACAGATATTCTTGCTAGGCTCAAACGTATGCAGGGTTACAATGTATTACACCCAATGGGATGGGATGCATTTGGATTGCCAGCGGAGCAATATGCGATCGAGACAGGAACCCACCCAAAGATCACAACCTTGAAGAACATTGACCGCTTTCGTTCCCAGCTTAAATCGTTAGGTTTCTCATATGACTGGGATCGTGAAATTTCTACAACAGAACCAGAATATTATAAATGGACCCAATGGATCTTTCTTCAGCTATTAAAGAGAGGCTTGGCATATCAGGCTGAAGTGCCAGTCAATTGGTGCCCTGCTCTTGGTACTGTGTTGGCAAATGAAGAAGTGATAGATGGTCTGAGCGAGCGTGGGGGTCATCCAGTTATAAGAAAGCCAATGAAGCAATGGATGCTCAAGATTACGGCATATGCTGATCGCCTTCTTGAAGATTTAGATGACCTTGACTGGCCTGAGAGTATCAAAGAGATGCAAAGAAACTGGATAGGGAAGTCAGAAGGCGCTGAGATGGAATTTTCTGTTCTTAGTAGTGATGGACAAGAGAGAGACATAAAGATTACAATATATACTACCAGACCGGACACCATCTTTGGAGCAACATATTTAGTCATCGCACCAGAGCATCCCTTGCTGTCATCATCAGTATCAACAGCCCAGAGAGAAAGTGTGGAGGGGTACGTAGATGTTGCCTCAAGAAAGAGTGACCTTGAGAGGACTGAGCTTCAGAAGGAAAAGACCGGGGTGTTCAGTGGTTGCTATGCTAAAAATCCAGTTAATGGAGAAGCTATCCCTATCTGGGTTGCGGACTATGTCTTGGCCAGTTATGGAACGGGAGCAATAATGGCTGTACCTGCACATGATGCTCGTGACTATGAATTTGCTTCAAAATATGATATCCCAATCCGTTGGGTTGTGATGCCGCACGATAAAAAGCTCAGTGGTTCCGGAAAGGCCTATTCAGGTGAAGGCAATGTTATAAATTCTTCAAACACAACATTGGGGCTGGACATTAATAGCTTATCTAGCAAAGAAGCTGCTTTCAAAGTCATTGAATGGGCCGAGAAAACTGGAAATGGGAAGAAAAAGGTGAACTATAAGTTGAGGGATTGGCTTTTTGCTAGGCAACGCTATTGGGGGGAACCTATTCCAGTAGTTTTTTCAGATGATAGTGATGAGGGTGTTCCCATTCATGAAACCGAACTGCCCCTCACCCTTCCAGAATTGGATGATTTTTCTCCCACTGGAACAGGAGAACCACCCCTAGCAAAAGCAGTTTCTTGGGTCAAAACTAAGGATCCTTTGTCTGGAAAACCTGCCAGACGAGAGACAAGCACCATGCCCCAGTGGGCTGGTTCTTGCTGGTACTATTTGAGATTTATGGACCCAAAAAATTCAAAAGAATTGGTGGCCAAGACAAAAGAAATGTATTGGAGCCCAGTTGATGTGTATGTTGGTGGTGCTGAACATGCTGTTCTCCATTTACTTTATGCACGATTCTGGCACAAGGTTCTCTATGACATTGGTGTCGTCTCCACCAAAGAACCCTTCAAGTGTGTCATAAATCAGGGCATTATTCTTGGAGAAGTTCAATATATCGCTTACAAGGACTCAGATGGGAACTTTGTCTCTGCAGACTCTGATACATCAGCTGAGCTTCAACAAGAAATAATACCGGAGGAAAAGGTCATTAAATCCGGGGATTCTTTTGTACTAAAAGACAATCCTGAGATCTCTCTTATTGCACGTTCTCATAAAATGAGTAAGAGTAGGGGAAATGTTGTCAATCCTGATGATGTTGTTTCTGAGTACGGGGCCGATTCACTTCGCTTATATGAAATGTTCATGGGGCCGTTAAGAGACTCAAAACAATGGAATACGAGTGGTATTGAAGGTGTTCATCGGTTTTTGGGAAGAGCCTGGAGGCTAGTTGTTGGTTCAACGTTATCTGATGGTACACTCAAAGAGGGAACGGTAGTGACTGATGAGGATCCCACTTCGCAGCAACTTCGTTCTCTACATAAGTGCATTGCCAAGGTAACAGAGGAAATAGAAGCGACGAGATTTAATACTGGAATTTCTGCAATGATGGAGTTCATCAATGTGGCATATAAGTGGAAAAAACATCCGAGGGTGATTATTGAAGCGTTTGTTTTGTTGCTCGCACCATATGCACCTCATATGGCTGAGGAGCTTTGGTTTCGCTTGGGACACTCGAAATCATTGGCATACGAGGCTTTTCCTAAGGTCAATCCTGCTTACTTGGAGGAATCTACTATTTTGCTACCGGTTCAGATCAATGGCAAGACGAGGGGTACAATCCAGGTTGAAAAGACCTGTTTGGAGGAGGATGCATTCCTACTGGCATCGAAAGATGGAAAGCTCTCCAAATATCTTGATGGGGTGTCAATTAAAAAGAGGATTTTCGTCCCTGGAAAAATCCTGAATGTCATCTTGGACCGTCAAAACGTCAAGGCAGCTGTGCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004823 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006429 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032543 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140053 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1 tRNA-synt_1_2

Protein Analysis

959

Amino Acids

108.23

Weight (kDa)

6.31

Isoelectric Point (pI)

39.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	81 - 294	3.4e-30	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	109 - 241	1.3e-17	tRNA synthetases class I (M)
tRNA-synt_1_2	PF13603	290 - 482	3.4e-62	Leucyl-tRNA synthetase, editing domain
tRNA-synt_1	PF00133	714 - 744	8.5e-07	tRNA synthetases class I (I, L, M and V)
Anticodon_1	PF08264	800 - 908	4.7e-17	Anticodon-binding domain of tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04350
fragaria_vesca	FvH4_3g01570 FvH4_3g01570
malus_domestica	MD10G1325600.v1.1
prunus_persica	Prupe.4G016400_v2.0.a1 Prupe.4G016400_v2.0.a1
pyrus_communis	pycom10g27530
rosa_chinensis	RchiOBHm_Chr5g0002381
rosa_laevigata	RLG00000031024
rosa_multiflora	Rmu_sc0006137.1_g000003 Rmu_sc0017945.1_g000003
rosa_roxburghii	Rroxscaffold_1G00073490
rosa_rugosa	Rorug04G0395800.1
rosa_samantha	Rh5AG018500 Rh5BG021200 Rh5DG019500
rosa_wichuraiana	Rw5G001740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	609, 761, 1370
AasI	GACNNNNNNGTC	1 cut(s)	1916
AatII	GACGTC	1 cut(s)	171
Acc36I	ACCTGC	2 cut(s)	1252, 1714
AccB1I	GGYRCC	1 cut(s)	684
AccB7I	CCANNNNNTGG	1 cut(s)	1169
AccBSI	CCGCTC	1 cut(s)	347
AccII	CGCG	1 cut(s)	129
AccIII	TCCGGA	1 cut(s)	1337
AciI	CCGC	9 cut(s)	112, 127, 200, 347, 368, 467, 529, 1196, 1313
AclI	AACGTT	1 cut(s)	2333
AclWI	GGATC	6 cut(s)	582, 632, 1682, 1695, 2375, 2388
AcoI	YGGCCR	3 cut(s)	361, 1209, 1800
AcsI	RAATTY	6 cut(s)	582, 905, 1268, 1372, 1783, 2466
AcuI	CTGAAG	3 cut(s)	617, 687, 1100
AcyI	GRCGYC	1 cut(s)	168
AfaI	GTAC	8 cut(s)	700, 1070, 1242, 1757, 2103, 2201, 2347, 2707
AfiI	CCNNNNNNNGG	4 cut(s)	695, 1169, 1189, 1697
AflIII	ACRYGT	3 cut(s)	294, 324, 355
AgeI	ACCGGT	1 cut(s)	2677
AjiI	CACGTC	1 cut(s)	295
AjnI	CCWGG	3 cut(s)	2309, 2712, 2821
AjuI	GAANNNNNNNTTGG	6 cut(s)	612, 644, 1922, 1954, 2828, 2860
Alw26I	GTCTC	7 cut(s)	32, 479, 933, 1712, 1927, 2023, 2244
AlwI	GGATC	6 cut(s)	582, 632, 1682, 1695, 2375, 2388
AlwNI	CAGNNNCTG	1 cut(s)	2030
Aor13HI	TCCGGA	1 cut(s)	1337
AoxI	GGCC	9 cut(s)	210, 361, 842, 1209, 1344, 1449, 1800, 2205, 2240
ApeKI	GCWGC	6 cut(s)	114, 190, 244, 1427, 2392, 2867
ApoI	RAATTY	6 cut(s)	582, 905, 1268, 1372, 1783, 2466
ArsI	GACNNNNNNTTYG	4 cut(s)	965, 997, 1253, 1285
AseI	ATTAAT	1 cut(s)	1404
AsiGI	ACCGGT	1 cut(s)	2677
Asp700I	GAANNNNTTC	2 cut(s)	1536, 2286
AspLEI	GCGC	1 cut(s)	896
AspS9I	GGNCC	7 cut(s)	210, 615, 1449, 1774, 2205, 2240, 2848
AsuC2I	CCSGG	3 cut(s)	281, 1132, 2089
AsuHPI	GGTGA	5 cut(s)	214, 1367, 1492, 1594, 2536
AsuII	TTCGAA	1 cut(s)	274
AvaII	GGWCC	3 cut(s)	615, 1774, 2848
AxyI	CCTNAGG	1 cut(s)	2634
BaeGI	GKGCMC	1 cut(s)	689
BalI	TGGCCA	3 cut(s)	363, 1211, 1802
BamHI	GGATCC	2 cut(s)	1687, 2380
BanI	GGYRCC	1 cut(s)	684
BanII	GRGCYC	2 cut(s)	237, 1829
BauI	CACGAG	1 cut(s)	1257
BbvCI	CCTCAGC	2 cut(s)	145, 2577
BbvI	GCAGC	5 cut(s)	126, 202, 256, 1414, 2404
BceAI	ACGGC	2 cut(s)	813, 2227
BcgI	CGANNNNNNTGC	2 cut(s)	2381, 2415
BciT130I	CCWGG	3 cut(s)	2311, 2714, 2823
BcnI	CCSGG	3 cut(s)	281, 1132, 2089
BcoDI	GTCTC	7 cut(s)	32, 479, 933, 1712, 1927, 2023, 2244
BfaI	CTAG	5 cut(s)	399, 1416, 1512, 1655, 2318
BfmI	CTRYAG	1 cut(s)	2022
BfoI	RGCGCY	1 cut(s)	897
BfuAI	ACCTGC	2 cut(s)	1252, 1714
BglI	GCCNNNNNGGC	1 cut(s)	241
BglII	AGATCT	1 cut(s)	2121
BisI	GCNGC	8 cut(s)	115, 127, 191, 245, 1313, 1428, 2393, 2868
BlpI	GCTNAGC	1 cut(s)	2043
BlsI	GCNGC	8 cut(s)	116, 128, 192, 246, 1314, 1429, 2394, 2869
Bme1390I	CCNGG	6 cut(s)	281, 1132, 2089, 2311, 2714, 2823
Bme18I	GGWCC	3 cut(s)	615, 1774, 2848
BmgBI	CACGTC	1 cut(s)	295
BmgT120I	GGNCC	7 cut(s)	210, 615, 1449, 1774, 2205, 2240, 2848
BmrFI	CCNGG	6 cut(s)	281, 1132, 2089, 2311, 2714, 2823
BmrI	ACTGGG	5 cut(s)	32, 333, 580, 1729, 1823
BmsI	GCATC	8 cut(s)	439, 774, 852, 1024, 1243, 1299, 2731, 2765
BmuI	ACTGGG	5 cut(s)	32, 333, 580, 1729, 1823
BplI	GAGNNNNNCTC	1 cut(s)	27
BpmI	CTGGAG	1 cut(s)	2332
Bpu10I	CCTNAGC	2 cut(s)	145, 2577
Bpu1102I	GCTNAGC	1 cut(s)	2043
Bpu14I	TTCGAA	1 cut(s)	274
BpuEI	CTTGAG	3 cut(s)	773, 1069, 1121
BpuMI	CCSGG	3 cut(s)	281, 1132, 2089
BsaAI	YACGTR	1 cut(s)	1072
BsaBI	GATNNNNATC	1 cut(s)	2379
BsaHI	GRCGYC	1 cut(s)	168
BsaI	GGTCTC	1 cut(s)	32
BsaJI	CCNNGG	6 cut(s)	279, 1131, 2088, 2424, 2519, 2821
BsaWI	WCCGGW	4 cut(s)	970, 1337, 2065, 2677
BsaXI	ACNNNNNCTCC	6 cut(s)	12, 42, 51, 81, 2573, 2603
Bsc4I	CCNNNNNNNGG	4 cut(s)	695, 1169, 1189, 1697
Bse118I	RCCGGY	1 cut(s)	2677
Bse21I	CCTNAGG	1 cut(s)	2634
Bse3DI	GCAATG	4 cut(s)	785, 1369, 2418, 2481
Bse8I	GATNNNNATC	1 cut(s)	2379
BseAI	TCCGGA	1 cut(s)	1337
BseBI	CCWGG	3 cut(s)	2311, 2714, 2823
BseDI	CCNNGG	6 cut(s)	279, 1131, 2088, 2424, 2519, 2821
BseJI	GATNNNNATC	1 cut(s)	2379
BseLI	CCNNNNNNNGG	4 cut(s)	695, 1169, 1189, 1697
BseMI	GCAATG	4 cut(s)	785, 1369, 2418, 2481
BseRI	GAGGAG	2 cut(s)	2594, 2750
BseSI	GKGCMC	1 cut(s)	689
BseXI	GCAGC	5 cut(s)	126, 202, 256, 1414, 2404
BseYI	CCCAGC	1 cut(s)	540
BsgI	GTGCAG	1 cut(s)	1230
Bsh1236I	CGCG	1 cut(s)	129
Bsh1285I	CGRYCG	1 cut(s)	483
BshFI	GGCC	9 cut(s)	212, 363, 844, 1211, 1346, 1451, 1802, 2207, 2242
BshNI	GGYRCC	1 cut(s)	684
BshTI	ACCGGT	1 cut(s)	2677
BsiEI	CGRYCG	1 cut(s)	483
BsiSI	CCGG	7 cut(s)	281, 971, 1131, 1338, 2066, 2088, 2678
BslFI	GGGAC	2 cut(s)	2613, 2804
BslI	CCNNNNNNNGG	4 cut(s)	695, 1169, 1189, 1697
BsmAI	GTCTC	7 cut(s)	32, 479, 933, 1712, 1927, 2023, 2244
BsmBI	CGTCTC	1 cut(s)	1927
BsmFI	GGGAC	2 cut(s)	2613, 2804
BsmI	GAATGC	1 cut(s)	2744
BsnI	GGCC	9 cut(s)	212, 363, 844, 1211, 1346, 1451, 1802, 2207, 2242
Bso31I	GGTCTC	1 cut(s)	32
Bsp119I	TTCGAA	1 cut(s)	274
Bsp1286I	GDGCHC	3 cut(s)	237, 689, 1829
Bsp13I	TCCGGA	1 cut(s)	1337
Bsp143I	GATC	9 cut(s)	480, 504, 574, 624, 808, 1687, 2121, 2380, 2685
Bsp1720I	GCTNAGC	1 cut(s)	2043
BspACI	CCGC	9 cut(s)	112, 127, 200, 347, 368, 467, 529, 1196, 1313
BspANI	GGCC	9 cut(s)	212, 363, 844, 1211, 1346, 1451, 1802, 2207, 2242
BspEI	TCCGGA	1 cut(s)	1337
BspFNI	CGCG	1 cut(s)	129
BspHI	TCATGA	1 cut(s)	1582
BspMAI	CTGCAG	1 cut(s)	2026
BspMI	ACCTGC	2 cut(s)	1252, 1714
BspPI	GGATC	6 cut(s)	582, 632, 1682, 1695, 2375, 2388
BspQI	GCTCTTC	1 cut(s)	2299
BspT104I	TTCGAA	1 cut(s)	274
BspT107I	GGYRCC	1 cut(s)	684
BspTNI	GGTCTC	1 cut(s)	32
BsrBI	CCGCTC	1 cut(s)	347
BsrDI	GCAATG	4 cut(s)	785, 1369, 2418, 2481
BsrFI	RCCGGY	1 cut(s)	2677
BssAI	RCCGGY	1 cut(s)	2677
BssECI	CCNNGG	6 cut(s)	279, 1131, 2088, 2424, 2519, 2821
BssMI	GATC	9 cut(s)	480, 504, 574, 624, 808, 1687, 2121, 2380, 2685
BssNI	GRCGYC	1 cut(s)	168
BssSI	CACGAG	1 cut(s)	1257
BssT1I	CCWWGG	1 cut(s)	2424
Bst2BI	CACGAG	1 cut(s)	1257
Bst2UI	CCWGG	3 cut(s)	2311, 2714, 2823
Bst4CI	ACNGT	3 cut(s)	703, 2365, 2852
Bst6I	CTCTTC	2 cut(s)	200, 2299
BstACI	GRCGYC	1 cut(s)	168
BstAPI	GCANNNNNTGC	4 cut(s)	784, 1021, 1253, 2561
BstBAI	YACGTR	1 cut(s)	1072
BstBI	TTCGAA	1 cut(s)	274
BstC8I	GCNNGC	3 cut(s)	465, 740, 2553
BstFNI	CGCG	1 cut(s)	129
BstH2I	RGCGCY	1 cut(s)	897
BstHHI	GCGC	1 cut(s)	896
BstKTI	GATC	9 cut(s)	483, 507, 577, 627, 811, 1690, 2124, 2383, 2688
BstMAI	GTCTC	7 cut(s)	32, 479, 933, 1712, 1927, 2023, 2244
BstMBI	GATC	9 cut(s)	480, 504, 574, 624, 808, 1687, 2121, 2380, 2685
BstMCI	CGRYCG	1 cut(s)	483
BstNI	CCWGG	3 cut(s)	2311, 2714, 2823
BstNSI	RCATGY	3 cut(s)	328, 359, 1862
BstSCI	CCNGG	6 cut(s)	279, 1130, 2087, 2309, 2712, 2821
BstSFI	CTRYAG	1 cut(s)	2022
BstSLI	GKGCMC	1 cut(s)	689
BstSNI	TACGTA	1 cut(s)	1072
BstUI	CGCG	1 cut(s)	129
BstV1I	GCAGC	5 cut(s)	126, 202, 256, 1414, 2404
BstX2I	RGATCY	4 cut(s)	624, 1687, 2121, 2380
BstXI	CCANNNNNNTGG	5 cut(s)	371, 444, 681, 1299, 1742
BstYI	RGATCY	4 cut(s)	624, 1687, 2121, 2380
Bsu36I	CCTNAGG	1 cut(s)	2634
BsuRI	GGCC	9 cut(s)	212, 363, 844, 1211, 1346, 1451, 1802, 2207, 2242
BtgZI	GCGATG	1 cut(s)	988
BtrI	CACGTC	1 cut(s)	295
BtsIMutI	CAGTG	5 cut(s)	346, 1147, 1336, 1632, 1742
BveI	ACCTGC	2 cut(s)	1252, 1714
Cac8I	GCNNGC	3 cut(s)	465, 740, 2553
CaiI	CAGNNNCTG	1 cut(s)	2030
CciI	TCATGA	1 cut(s)	1582
CfoI	GCGC	1 cut(s)	896
Cfr10I	RCCGGY	1 cut(s)	2677
Cfr13I	GGNCC	7 cut(s)	210, 615, 1449, 1774, 2205, 2240, 2848
Csp6I	GTAC	8 cut(s)	699, 1069, 1241, 1756, 2102, 2200, 2346, 2706
CspAI	ACCGGT	1 cut(s)	2677
CviAII	CATG	7 cut(s)	325, 356, 1250, 1583, 1729, 1859, 2236
CviQI	GTAC	8 cut(s)	699, 1069, 1241, 1756, 2102, 2200, 2346, 2706
DpnI	GATC	9 cut(s)	482, 506, 576, 626, 810, 1689, 2123, 2382, 2687
DpnII	GATC	9 cut(s)	480, 504, 574, 624, 808, 1687, 2121, 2380, 2685
DrdI	GACNNNNNNGTC	1 cut(s)	1916
DseDI	GACNNNNNNGTC	1 cut(s)	1916
EaeI	YGGCCR	3 cut(s)	361, 1209, 1800
Eam1104I	CTCTTC	2 cut(s)	200, 2299
EarI	CTCTTC	2 cut(s)	200, 2299
Eco105I	TACGTA	1 cut(s)	1072
Eco130I	CCWWGG	1 cut(s)	2424
Eco147I	AGGCCT	1 cut(s)	1346
Eco24I	GRGCYC	2 cut(s)	237, 1829
Eco31I	GGTCTC	1 cut(s)	32
Eco32I	GATATC	1 cut(s)	1288
Eco47I	GGWCC	3 cut(s)	615, 1774, 2848
Eco57I	CTGAAG	3 cut(s)	617, 687, 1100
Eco81I	CCTNAGG	1 cut(s)	2634
EcoO109I	RGGNCCY	1 cut(s)	210
EcoRII	CCWGG	3 cut(s)	2309, 2712, 2821
EcoRV	GATATC	1 cut(s)	1288
EcoT14I	CCWWGG	1 cut(s)	2424
EcoT22I	ATGCAT	2 cut(s)	454, 2746
EcoT38I	GRGCYC	2 cut(s)	237, 1829
ErhI	CCWWGG	1 cut(s)	2424
Esp3I	CGTCTC	1 cut(s)	1927
FaeI	CATG	7 cut(s)	328, 359, 1253, 1586, 1732, 1862, 2239
FalI	AAGNNNNNCTT	2 cut(s)	635, 667
FaqI	GGGAC	2 cut(s)	2613, 2804
FatI	CATG	7 cut(s)	324, 355, 1249, 1582, 1728, 1858, 2235
FauI	CCCGC	1 cut(s)	340
FauNDI	CATATG	4 cut(s)	565, 802, 2560, 2571
Fnu4HI	GCNGC	8 cut(s)	115, 127, 191, 245, 1313, 1428, 2393, 2868
FriOI	GRGCYC	2 cut(s)	237, 1829
Fsp4HI	GCNGC	8 cut(s)	115, 127, 191, 245, 1313, 1428, 2393, 2868
FspBI	CTAG	5 cut(s)	399, 1416, 1512, 1655, 2318
GlaI	GCGC	1 cut(s)	895
GluI	GCNGC	8 cut(s)	115, 127, 191, 245, 1313, 1428, 2393, 2868
GsaI	CCCAGC	1 cut(s)	544
GsuI	CTGGAG	1 cut(s)	2332
HaeII	RGCGCY	1 cut(s)	897
HaeIII	GGCC	9 cut(s)	212, 363, 844, 1211, 1346, 1451, 1802, 2207, 2242
HapII	CCGG	7 cut(s)	281, 971, 1131, 1338, 2066, 2088, 2678
HhaI	GCGC	1 cut(s)	896
Hin1I	GRCGYC	1 cut(s)	168
Hin1II	CATG	7 cut(s)	328, 359, 1253, 1586, 1732, 1862, 2239
Hin6I	GCGC	1 cut(s)	894
HinP1I	GCGC	1 cut(s)	894
HincII	GTYRAC	1 cut(s)	292
HindII	GTYRAC	1 cut(s)	292
HinfI	GANTC	8 cut(s)	102, 1887, 2000, 2027, 2093, 2210, 2252, 2660
HpaII	CCGG	7 cut(s)	281, 971, 1131, 1338, 2066, 2088, 2678
HphI	GGTGA	5 cut(s)	214, 1367, 1492, 1594, 2536
Hpy166II	GTNNAC	3 cut(s)	292, 1483, 2348
Hpy8I	GTNNAC	3 cut(s)	292, 1483, 2348
Hpy99I	CGWCG	3 cut(s)	170, 173, 2452
HpyAV	CCTTC	8 cut(s)	61, 824, 884, 1114, 1352, 1616, 1948, 2276
HpyCH4III	ACNGT	3 cut(s)	703, 2365, 2852
HpyCH4IV	ACGT	7 cut(s)	168, 294, 409, 1071, 2137, 2333, 2859
HpySE526I	ACGT	7 cut(s)	168, 294, 409, 1071, 2137, 2333, 2859
Hsp92I	GRCGYC	1 cut(s)	168
Hsp92II	CATG	7 cut(s)	328, 359, 1253, 1586, 1732, 1862, 2239
HspAI	GCGC	1 cut(s)	894
Kpn2I	TCCGGA	1 cut(s)	1337
Kzo9I	GATC	9 cut(s)	480, 504, 574, 624, 808, 1687, 2121, 2380, 2685
LguI	GCTCTTC	1 cut(s)	2299
LmnI	GCTCC	6 cut(s)	344, 469, 986, 1226, 1824, 2581
Lsp1109I	GCAGC	5 cut(s)	126, 202, 256, 1414, 2404
LweI	GCATC	8 cut(s)	439, 774, 852, 1024, 1243, 1299, 2731, 2765
MaeI	CTAG	5 cut(s)	399, 1416, 1512, 1655, 2318
MaeII	ACGT	7 cut(s)	168, 294, 409, 1071, 2137, 2333, 2859
MaeIII	GTNAC	6 cut(s)	351, 419, 1094, 1259, 2368, 2428
MalI	GATC	9 cut(s)	482, 506, 576, 626, 810, 1689, 2123, 2382, 2687
MbiI	CCGCTC	1 cut(s)	347
MboI	GATC	9 cut(s)	480, 504, 574, 624, 808, 1687, 2121, 2380, 2685
MboII	GAAGA	9 cut(s)	217, 272, 529, 623, 728, 833, 1368, 1483, 2316
MfeI	CAATTG	1 cut(s)	679
MflI	RGATCY	4 cut(s)	624, 1687, 2121, 2380
MhlI	GDGCHC	3 cut(s)	237, 689, 1829
MlsI	TGGCCA	3 cut(s)	363, 1211, 1802
MluNI	TGGCCA	3 cut(s)	363, 1211, 1802
MlyI	GAGTC	4 cut(s)	96, 1994, 2021, 2246
MmeI	TCCRAC	1 cut(s)	55
Mox20I	TGGCCA	3 cut(s)	363, 1211, 1802
Mph1103I	ATGCAT	2 cut(s)	454, 2746
MroI	TCCGGA	1 cut(s)	1337
MroXI	GAANNNNTTC	2 cut(s)	1536, 2286
MscI	TGGCCA	3 cut(s)	363, 1211, 1802
MseI	TTAA	8 cut(s)	546, 641, 1167, 1404, 2082, 2246, 2457, 2802
MslI	CAYNNNNRTG	2 cut(s)	2492, 2503
Msp20I	TGGCCA	3 cut(s)	363, 1211, 1802
MspA1I	CMGCKG	3 cut(s)	467, 2042, 2870
MspI	CCGG	7 cut(s)	281, 971, 1131, 1338, 2066, 2088, 2678
MspR9I	CCNGG	6 cut(s)	281, 1132, 2089, 2311, 2714, 2823
MunI	CAATTG	1 cut(s)	679
Mva1269I	GAATGC	1 cut(s)	2744
MvaI	CCWGG	3 cut(s)	2311, 2714, 2823
MvnI	CGCG	1 cut(s)	129
NciI	CCSGG	3 cut(s)	281, 1132, 2089
NdeI	CATATG	4 cut(s)	565, 802, 2560, 2571
NdeII	GATC	9 cut(s)	480, 504, 574, 624, 808, 1687, 2121, 2380, 2685
NlaIII	CATG	7 cut(s)	328, 359, 1253, 1586, 1732, 1862, 2239
NmeAIII	GCCGAG	1 cut(s)	1477
NmuCI	GTSAC	3 cut(s)	1094, 1259, 2368
NsiI	ATGCAT	2 cut(s)	454, 2746
NspI	RCATGY	3 cut(s)	328, 359, 1862
NspV	TTCGAA	1 cut(s)	274
PagI	TCATGA	1 cut(s)	1582
PceI	AGGCCT	1 cut(s)	1346
PciI	ACATGT	2 cut(s)	324, 355
PciSI	GCTCTTC	1 cut(s)	2299
PctI	GAATGC	1 cut(s)	2744
PdmI	GAANNNNTTC	2 cut(s)	1536, 2286
PfeI	GAWTC	4 cut(s)	1887, 2093, 2210, 2660
PflFI	GACNNNGTC	1 cut(s)	1202
PflMI	CCANNNNNTGG	1 cut(s)	1169
PinAI	ACCGGT	1 cut(s)	2677
PkrI	GCNGC	8 cut(s)	116, 128, 192, 246, 1314, 1429, 2394, 2869
Ple19I	CGATCG	1 cut(s)	483
PleI	GAGTC	4 cut(s)	96, 1994, 2021, 2246
PpsI	GAGTC	4 cut(s)	96, 1994, 2021, 2246
Ppu21I	YACGTR	1 cut(s)	1072
PscI	ACATGT	2 cut(s)	324, 355
PshBI	ATTAAT	1 cut(s)	1404
PsiI	TTATAA	3 cut(s)	609, 761, 1370
Psp1406I	AACGTT	1 cut(s)	2333
Psp6I	CCWGG	3 cut(s)	2309, 2712, 2821
PspFI	CCCAGC	1 cut(s)	540
PspGI	CCWGG	3 cut(s)	2309, 2712, 2821
PspPI	GGNCC	7 cut(s)	210, 615, 1449, 1774, 2205, 2240, 2848
PstI	CTGCAG	1 cut(s)	2026
PstNI	CAGNNNCTG	1 cut(s)	2030
PsuI	RGATCY	4 cut(s)	624, 1687, 2121, 2380
PsyI	GACNNNGTC	1 cut(s)	1202
PvuI	CGATCG	1 cut(s)	483
PvuII	CAGCTG	2 cut(s)	2042, 2870
RsaI	GTAC	8 cut(s)	700, 1070, 1242, 1757, 2103, 2201, 2347, 2707
RsaNI	GTAC	8 cut(s)	699, 1069, 1241, 1756, 2102, 2200, 2346, 2706
RseI	CAYNNNNRTG	2 cut(s)	2492, 2503
SapI	GCTCTTC	1 cut(s)	2299
SaqAI	TTAA	8 cut(s)	546, 641, 1167, 1404, 2082, 2246, 2457, 2802
SatI	GCNGC	8 cut(s)	115, 127, 191, 245, 1313, 1428, 2393, 2868
Sau3AI	GATC	9 cut(s)	480, 504, 574, 624, 808, 1687, 2121, 2380, 2685
Sau96I	GGNCC	7 cut(s)	210, 615, 1449, 1774, 2205, 2240, 2848
SchI	GAGTC	4 cut(s)	96, 1994, 2021, 2246
ScrFI	CCNGG	6 cut(s)	281, 1132, 2089, 2311, 2714, 2823
SduI	GDGCHC	3 cut(s)	237, 689, 1829
SfaNI	GCATC	8 cut(s)	439, 774, 852, 1024, 1243, 1299, 2731, 2765
SfcI	CTRYAG	1 cut(s)	2022
SfuI	TTCGAA	1 cut(s)	274
SinI	GGWCC	3 cut(s)	615, 1774, 2848
SmiMI	CAYNNNNRTG	2 cut(s)	2492, 2503
SmlI	CTYRAG	3 cut(s)	788, 1084, 1100
SmoI	CTYRAG	3 cut(s)	788, 1084, 1100
SnaBI	TACGTA	1 cut(s)	1072
SseBI	AGGCCT	1 cut(s)	1346
SsiI	CCGC	9 cut(s)	112, 127, 200, 347, 368, 467, 529, 1196, 1313
SspI	AATATT	1 cut(s)	605
SspMI	CTAG	5 cut(s)	399, 1416, 1512, 1655, 2318
StuI	AGGCCT	1 cut(s)	1346
StyD4I	CCNGG	6 cut(s)	279, 1130, 2087, 2309, 2712, 2821
StyI	CCWWGG	1 cut(s)	2424
TaaI	ACNGT	3 cut(s)	703, 2365, 2852
TaiI	ACGT	7 cut(s)	171, 297, 412, 1074, 2140, 2336, 2862
TaqI	TCGA	5 cut(s)	231, 274, 483, 2606, 2759
TatI	WGTACW	1 cut(s)	2101
TauI	GCSGC	2 cut(s)	129, 1315
TfiI	GAWTC	4 cut(s)	1887, 2093, 2210, 2660
Tru1I	TTAA	8 cut(s)	546, 641, 1167, 1404, 2082, 2246, 2457, 2802
Tru9I	TTAA	8 cut(s)	546, 641, 1167, 1404, 2082, 2246, 2457, 2802
TscAI	CASTG	5 cut(s)	346, 1147, 1336, 1639, 1742
TseFI	GTSAC	3 cut(s)	1094, 1259, 2368
TseI	GCWGC	6 cut(s)	114, 190, 244, 1427, 2392, 2867
Tsp45I	GTSAC	3 cut(s)	1094, 1259, 2368
TspGWI	ACGGA	1 cut(s)	1286
TspRI	CASTG	5 cut(s)	346, 1147, 1336, 1639, 1742
Tth111I	GACNNNGTC	1 cut(s)	1202
Van91I	CCANNNNNTGG	1 cut(s)	1169
VpaK11BI	GGWCC	3 cut(s)	615, 1774, 2848
VspI	ATTAAT	1 cut(s)	1404
XapI	RAATTY	6 cut(s)	582, 905, 1268, 1372, 1783, 2466
XceI	RCATGY	3 cut(s)	328, 359, 1862
XcmI	CCANNNNNNNNNTGG	1 cut(s)	777
XmnI	GAANNNNTTC	2 cut(s)	1536, 2286
XspI	CTAG	5 cut(s)	399, 1416, 1512, 1655, 2318
ZraI	GACGTC	1 cut(s)	169
Zsp2I	ATGCAT	2 cut(s)	454, 2746

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.