Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0014382

Physical Location & Seq

Genomic Coordinates Reverse (-)

94323026 .. 94330279

Gene Structure 7254 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00073490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2850 bp

ATGAGTTCTACTCACACCCAACTGGGTCTCCAACTCCAACTCCAACCTCGCCTAACCTTCAACTCACTTCTACTCCCCTCACACCCACTCATCACTTTCTCCCCCAGACTCCACAACCGCAGCTTCAAACGCCGCGTTTCCATCGCAGCCTCAGCTTCCAATGAACCCAACGACGTCGTAGAGCAGAGGCCGAAGCAGCAATCGGCGGTGAAGAGGGCTTATCCTTTCCACGAAATCGAGCCCAAGTGGCAGCGTTATTGGGAGGAAGATGGGACTTTTCGAACGCCAGAGGAAGTTGACATGTCAAAGCCCAAGTTCTATGTTCTTGACATGTTCCCTTATCCCAGTGGAGCGGGGTTACATGTTGGCCATCCGCTCGGATATACTGCGACAGATATTCTTGCTAGGCTCAAACGTATGCAGGGTTACAATGTATTACACCCAATGGGATGGGATGCATTTGGATTGCCAGCGGAGCAATATGCGATCGAGACAGGAACCCACCCAAAGATCACAACCTTGAAGAACATTGACCGCTTTCGTTCCCAGCTTAAATCGTTAGGTTTCTCATATGACTGGGATCGTGAAATTTCTACAACAGAACCAGAATATTATAAATGGACCCAGTGGATCTTTCTTCAGCTATTAAAGAGAGGCTTGGCGTATCAGGCTGAAGTGCCAGTCAATTGGTGCCCTGCTCTTGGTACTGTCTTGGCAAATGAAGAAGTGATAGATGGTCTGAGCGAGCGTGGGGGTCATCCAGTTATAAGAAAGCCAATGAAGCAATGGATGCTTAAGATTACGGCATATGCTGATCGCCTTCTTGAAGATCTAGATGACCTTGACTGGCCTGAGAGTATCAAAGAGATGCAAAGAAACTGGATAGGGAAGTCAGAAGGCGCTGAGATGGAATTTTCTGTTCTTAGTAGTGATGGACAAGAGAGAGACATAAAGATTACAATATATACTACCAGACCGGACACCATCTTTGGAGCAACATATTTAGTCATCGCACCGGAGCATCCCTTGTTGTCATCATTAGTATCAACAGCCCAGAGAGAAAGTGTGGAGGAGTACATAGATGTTGCCTCAAGAAAGAGTGACCTTGAGAGGACTGAGCTTCAGAAGGAAAAGACCGGGGTGTTCAGTGGTTGCTATGCTAAAAATCCAGTTAATGGAGAAGCTATCCCTATCTGGGTTGCGGACTATGTCTTGGGCAGTTATGGAACGGGAGCAATAATGGCTGTACCTGCACATGACACTCGTGACTATGAATTTGCTTCAAAATATGATATCCCAATCCGTTGGGTTGTGATGCCGCACGATAAACAGCTCAGTGGTTCCGGAAAGGCCTATTCAGGTGAAGGCAATGTTATAAATTCTTCAAACACAACATTGGGGCTGGACATTAATAGCTTATCTAGCAAAGAAGCTGCTTTCAAAGTCATTGAATGGGCCGAGAAAACTGGAAATGGGAAGAAAAAGGCAACGTTATTGGGGGAACCTATTCCAGTAGTTTTTTCAGATGATGGTGATGAGGGTATTCCCATTCATGAAACCGAACTGCCCCTCACCCTTCCAGAATTGGATGATTTTTCTCCCACTGGAACAGGAGAACCACCCCTAGCAAAAGCAGTTTCTTGGGTCAAAACTAAGGATCCTTTGTCTGGAAAACCTGCCAGACGAGAGACAAGCACCATGCCCCAGTGGGCTGGTTCTTGCTGGTACTATTTGAGATTTATGGACCCAAAAAATTCAAAAGAATTGGTGGCCAAGACAAAAGAAATGTATTGGAGCCCAGTTGATGTGTATGTTGGTGGTGCTGAACATGCTGTTCTCCATTTACTTTATGCAAGATTCTGGCACAAGGTTCTCTATGACATTGGTGTCGTCTCCACCAAAGAACCCTTCAAGTGTGTCATAAATCAGGGCATTATTCTTGGAGAAGTTCAATATATTGCTTACAAGGACTCAGATGGGAACTTTGTCTCTGCAGACTCTGATACGTCAGCTGAGCTTCAACAAGAAATAATACCGGAGGAAAAGGTCATTAAATCCGGGGATTCTTTTGTACTAAAAGACAATCCTGAGATCTCTCTTATTGCACGTTCTCATAAAATGAGTAAGAGTAGGGGAAATGTTGTCAATCCTGATGATGTTGTTTCTGAGTACGGGGCTGATTCACTTCGCTTATATGAAATGTTCATGGGGCCATTAAGAGACTCAAAACAATGGAATACGAGTGGTATTGAAGGTGTTCATCGGTTTTTGGGAAGAGCCTGGAGACTAGTTGTTGGTTCAACATTATCTGATGGTACACTCAAAGAGGGAACAGTAGTGACTGATGAGGATCCCACTTCAGAGCAACTTCGTTCTCTACATAAGTGCATTGCCAAGGTAACAGAGGAAATAGAAGCGACAAGATTTAATACTGGAATTTCTGCAATGATGGAGTTCATCAATGTAGCATATAAGTGGAAAAAACATCCGAGAGTGATTATTGAAGCATTTGTTTTATTGCTTGCACCATATGCACCTCATATGGCTGAGGAGCTTTGGTTTCGCTTGGGACACTCGAAATCATTGGCATACGAGGCTCTTCCTAAGGTCAATCCTGCTTACTTGGAAGAATCTACTATTTTGCTACCAGTTCAGATCAATGGCAAGACGAGGGGTACAATCCAGGTTGAAAAGACGTGTTCCGAGGAGGATGCATTCCTACTGGCATTGAAAGATGAAAAGCTCTCCAAATATCTTGATGGGGTGTCAATTAAAAAGAGGATTTTCGTCCCTGGAAAAATCCTGAATGTCATCTTGGACCGTCAAAACGTCAAGGCAGCTGTGCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004823 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006429 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032543 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140053 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1 tRNA-synt_1_2

Protein Analysis

949

Amino Acids

106.89

Weight (kDa)

5.91

Isoelectric Point (pI)

41.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	83 - 296	1.6e-30	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	111 - 243	1.3e-17	tRNA synthetases class I (M)
tRNA-synt_1_2	PF13603	292 - 484	4.4e-63	Leucyl-tRNA synthetase, editing domain
tRNA-synt_1	PF00133	704 - 734	8.4e-07	tRNA synthetases class I (I, L, M and V)
Anticodon_1	PF08264	790 - 898	1.5e-16	Anticodon-binding domain of tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04350
fragaria_vesca	FvH4_3g01570 FvH4_3g01570
malus_domestica	MD10G1325600.v1.1
prunus_persica	Prupe.4G016400_v2.0.a1 Prupe.4G016400_v2.0.a1
pyrus_communis	pycom10g27530
rosa_chinensis	RchiOBHm_Chr5g0002381
rosa_laevigata	RLG00000031024
rosa_multiflora	Rmu_sc0006137.1_g000003 Rmu_sc0017945.1_g000003
rosa_roxburghii	Rroxscaffold_1G00073490
rosa_rugosa	Rorug04G0395800.1
rosa_samantha	Rh5AG018500 Rh5BG021200 Rh5DG019500
rosa_wichuraiana	Rw5G001740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	615, 767, 1376
AasI	GACNNNNNNGTC	1 cut(s)	1886
AatII	GACGTC	1 cut(s)	177
Acc36I	ACCTGC	2 cut(s)	1258, 1684
AccB1I	GGYRCC	1 cut(s)	690
AccB7I	CCANNNNNTGG	1 cut(s)	1175
AccBSI	CCGCTC	2 cut(s)	353, 376
AccII	CGCG	1 cut(s)	135
AccIII	TCCGGA	1 cut(s)	1343
AciI	CCGC	9 cut(s)	118, 133, 206, 353, 374, 473, 535, 1202, 1319
AclI	AACGTT	1 cut(s)	1490
AclWI	GGATC	6 cut(s)	588, 638, 1652, 1665, 2345, 2358
AcoI	YGGCCR	2 cut(s)	367, 1770
AcsI	RAATTY	6 cut(s)	588, 911, 1274, 1378, 1753, 2436
AcuI	CTGAAG	4 cut(s)	623, 693, 1106, 2343
AcyI	GRCGYC	1 cut(s)	174
AfaI	GTAC	8 cut(s)	706, 1076, 1248, 1727, 2073, 2171, 2317, 2677
AfiI	CCNNNNNNNGG	4 cut(s)	701, 1175, 1195, 1667
AflII	CTTAAG	1 cut(s)	794
AflIII	ACRYGT	4 cut(s)	300, 330, 361, 2696
AhlI	ACTAGT	1 cut(s)	2287
AjiI	CACGTC	1 cut(s)	2697
AjnI	CCWGG	3 cut(s)	2279, 2682, 2791
AjuI	GAANNNNNNNTTGG	4 cut(s)	1892, 1924, 2798, 2830
Alw26I	GTCTC	8 cut(s)	32, 485, 939, 1682, 1897, 1993, 2214, 2278
AlwI	GGATC	6 cut(s)	588, 638, 1652, 1665, 2345, 2358
AlwNI	CAGNNNCTG	1 cut(s)	2000
Aor13HI	TCCGGA	1 cut(s)	1343
AoxI	GGCC	7 cut(s)	188, 367, 848, 1350, 1455, 1770, 2210
ApeKI	GCWGC	6 cut(s)	120, 146, 196, 250, 1433, 2837
ApoI	RAATTY	6 cut(s)	588, 911, 1274, 1378, 1753, 2436
ArsI	GACNNNNNNTTYG	4 cut(s)	971, 1003, 1259, 1291
AseI	ATTAAT	1 cut(s)	1410
Asp700I	GAANNNNTTC	2 cut(s)	1506, 2256
AspLEI	GCGC	1 cut(s)	902
AspS9I	GGNCC	5 cut(s)	621, 1455, 1744, 2210, 2818
AsuC2I	CCSGG	2 cut(s)	1138, 2059
AsuHPI	GGTGA	4 cut(s)	220, 1373, 1544, 1564
AsuII	TTCGAA	1 cut(s)	280
AvaII	GGWCC	3 cut(s)	621, 1744, 2818
AxyI	CCTNAGG	1 cut(s)	2604
BaeGI	GKGCMC	1 cut(s)	695
BalI	TGGCCA	2 cut(s)	369, 1772
BamHI	GGATCC	2 cut(s)	1657, 2350
BanI	GGYRCC	1 cut(s)	690
BanII	GRGCYC	2 cut(s)	243, 1799
BauI	CACGAG	1 cut(s)	1263
BbvCI	CCTCAGC	2 cut(s)	151, 2547
BbvI	GCAGC	5 cut(s)	132, 158, 208, 262, 1420
BceAI	ACGGC	1 cut(s)	819
BcgI	CGANNNNNNTGC	2 cut(s)	2693, 2727
BciT130I	CCWGG	3 cut(s)	2281, 2684, 2793
BcnI	CCSGG	2 cut(s)	1138, 2059
BcoDI	GTCTC	8 cut(s)	32, 485, 939, 1682, 1897, 1993, 2214, 2278
BcuI	ACTAGT	1 cut(s)	2287
BfaI	CTAG	5 cut(s)	405, 833, 1422, 1625, 2288
BfmI	CTRYAG	1 cut(s)	1992
BfoI	RGCGCY	1 cut(s)	903
BfrI	CTTAAG	1 cut(s)	794
BfuAI	ACCTGC	2 cut(s)	1258, 1684
BglI	GCCNNNNNGGC	1 cut(s)	247
BglII	AGATCT	2 cut(s)	829, 2091
BisI	GCNGC	8 cut(s)	121, 133, 147, 197, 251, 1319, 1434, 2838
BlpI	GCTNAGC	1 cut(s)	2013
BlsI	GCNGC	8 cut(s)	122, 134, 148, 198, 252, 1320, 1435, 2839
Bme1390I	CCNGG	5 cut(s)	1138, 2059, 2281, 2684, 2793
Bme18I	GGWCC	3 cut(s)	621, 1744, 2818
BmgBI	CACGTC	1 cut(s)	2697
BmgT120I	GGNCC	5 cut(s)	621, 1455, 1744, 2210, 2818
BmrFI	CCNGG	5 cut(s)	1138, 2059, 2281, 2684, 2793
BmrI	ACTGGG	6 cut(s)	32, 339, 586, 619, 1699, 1793
BmsI	GCATC	6 cut(s)	445, 780, 858, 1030, 1305, 2701
BmuI	ACTGGG	6 cut(s)	32, 339, 586, 619, 1699, 1793
BplI	GAGNNNNNCTC	1 cut(s)	27
BpmI	CTGGAG	1 cut(s)	2302
Bpu10I	CCTNAGC	2 cut(s)	151, 2547
Bpu1102I	GCTNAGC	1 cut(s)	2013
Bpu14I	TTCGAA	1 cut(s)	280
BpuEI	CTTGAG	2 cut(s)	1075, 1127
BpuMI	CCSGG	2 cut(s)	1138, 2059
BsaBI	GATNNNNATC	1 cut(s)	2349
BsaHI	GRCGYC	1 cut(s)	174
BsaI	GGTCTC	1 cut(s)	32
BsaJI	CCNNGG	5 cut(s)	1137, 2058, 2394, 2703, 2791
BsaWI	WCCGGW	4 cut(s)	976, 1015, 1343, 2035
BsaXI	ACNNNNNCTCC	8 cut(s)	12, 24, 42, 54, 57, 87, 2543, 2573
Bsc4I	CCNNNNNNNGG	4 cut(s)	701, 1175, 1195, 1667
Bse21I	CCTNAGG	1 cut(s)	2604
Bse3DI	GCAATG	4 cut(s)	791, 1375, 2388, 2451
Bse8I	GATNNNNATC	1 cut(s)	2349
BseAI	TCCGGA	1 cut(s)	1343
BseBI	CCWGG	3 cut(s)	2281, 2684, 2793
BseDI	CCNNGG	5 cut(s)	1137, 2058, 2394, 2703, 2791
BseGI	GGATG	9 cut(s)	370, 455, 460, 757, 795, 1021, 1594, 2485, 2716
BseJI	GATNNNNATC	1 cut(s)	2349
BseLI	CCNNNNNNNGG	4 cut(s)	701, 1175, 1195, 1667
BseMI	GCAATG	4 cut(s)	791, 1375, 2388, 2451
BseRI	GAGGAG	3 cut(s)	1085, 2564, 2720
BseSI	GKGCMC	1 cut(s)	695
BseXI	GCAGC	5 cut(s)	132, 158, 208, 262, 1420
BseYI	CCCAGC	1 cut(s)	546
BsgI	GTGCAG	1 cut(s)	1236
Bsh1236I	CGCG	1 cut(s)	135
Bsh1285I	CGRYCG	1 cut(s)	489
BshFI	GGCC	7 cut(s)	190, 369, 850, 1352, 1457, 1772, 2212
BshNI	GGYRCC	1 cut(s)	690
BsiEI	CGRYCG	1 cut(s)	489
BsiSI	CCGG	6 cut(s)	977, 1016, 1137, 1344, 2036, 2058
BslFI	GGGAC	3 cut(s)	286, 2583, 2774
BslI	CCNNNNNNNGG	4 cut(s)	701, 1175, 1195, 1667
BsmAI	GTCTC	8 cut(s)	32, 485, 939, 1682, 1897, 1993, 2214, 2278
BsmBI	CGTCTC	1 cut(s)	1897
BsmFI	GGGAC	3 cut(s)	286, 2583, 2774
BsmI	GAATGC	1 cut(s)	2714
BsnI	GGCC	7 cut(s)	190, 369, 850, 1352, 1457, 1772, 2212
Bso31I	GGTCTC	1 cut(s)	32
Bsp119I	TTCGAA	1 cut(s)	280
Bsp1286I	GDGCHC	3 cut(s)	243, 695, 1799
Bsp13I	TCCGGA	1 cut(s)	1343
Bsp1720I	GCTNAGC	1 cut(s)	2013
BspACI	CCGC	9 cut(s)	118, 133, 206, 353, 374, 473, 535, 1202, 1319
BspANI	GGCC	7 cut(s)	190, 369, 850, 1352, 1457, 1772, 2212
BspEI	TCCGGA	1 cut(s)	1343
BspFNI	CGCG	1 cut(s)	135
BspHI	TCATGA	1 cut(s)	1552
BspMAI	CTGCAG	1 cut(s)	1996
BspMI	ACCTGC	2 cut(s)	1258, 1684
BspPI	GGATC	6 cut(s)	588, 638, 1652, 1665, 2345, 2358
BspQI	GCTCTTC	2 cut(s)	2269, 2604
BspT104I	TTCGAA	1 cut(s)	280
BspT107I	GGYRCC	1 cut(s)	690
BspTI	CTTAAG	1 cut(s)	794
BspTNI	GGTCTC	1 cut(s)	32
BsrBI	CCGCTC	2 cut(s)	353, 376
BsrDI	GCAATG	4 cut(s)	791, 1375, 2388, 2451
BssECI	CCNNGG	5 cut(s)	1137, 2058, 2394, 2703, 2791
BssNI	GRCGYC	1 cut(s)	174
BssSI	CACGAG	1 cut(s)	1263
BssT1I	CCWWGG	1 cut(s)	2394
Bst2BI	CACGAG	1 cut(s)	1263
Bst2UI	CCWGG	3 cut(s)	2281, 2684, 2793
Bst4CI	ACNGT	3 cut(s)	709, 2335, 2822
Bst6I	CTCTTC	3 cut(s)	206, 2269, 2604
BstACI	GRCGYC	1 cut(s)	174
BstAFI	CTTAAG	1 cut(s)	794
BstAPI	GCANNNNNTGC	2 cut(s)	790, 2531
BstBI	TTCGAA	1 cut(s)	280
BstC8I	GCNNGC	3 cut(s)	471, 746, 2523
BstF5I	GGATG	9 cut(s)	370, 455, 460, 757, 795, 1021, 1594, 2485, 2716
BstFNI	CGCG	1 cut(s)	135
BstH2I	RGCGCY	1 cut(s)	903
BstHHI	GCGC	1 cut(s)	902
BstMAI	GTCTC	8 cut(s)	32, 485, 939, 1682, 1897, 1993, 2214, 2278
BstMCI	CGRYCG	1 cut(s)	489
BstNI	CCWGG	3 cut(s)	2281, 2684, 2793
BstNSI	RCATGY	4 cut(s)	304, 334, 365, 1832
BstSCI	CCNGG	5 cut(s)	1136, 2057, 2279, 2682, 2791
BstSFI	CTRYAG	1 cut(s)	1992
BstSLI	GKGCMC	1 cut(s)	695
BstUI	CGCG	1 cut(s)	135
BstV1I	GCAGC	5 cut(s)	132, 158, 208, 262, 1420
BstX2I	RGATCY	5 cut(s)	630, 829, 1657, 2091, 2350
BstXI	CCANNNNNNTGG	4 cut(s)	450, 687, 1305, 1712
BstYI	RGATCY	5 cut(s)	630, 829, 1657, 2091, 2350
Bsu36I	CCTNAGG	1 cut(s)	2604
BsuRI	GGCC	7 cut(s)	190, 369, 850, 1352, 1457, 1772, 2212
BtgZI	GCGATG	2 cut(s)	127, 994
BtrI	CACGTC	1 cut(s)	2697
BtsCI	GGATG	9 cut(s)	370, 455, 460, 757, 795, 1021, 1594, 2485, 2716
BtsIMutI	CAGTG	6 cut(s)	352, 632, 1153, 1342, 1602, 1712
BveI	ACCTGC	2 cut(s)	1258, 1684
Cac8I	GCNNGC	3 cut(s)	471, 746, 2523
CaiI	CAGNNNCTG	1 cut(s)	2000
CciI	TCATGA	1 cut(s)	1552
CfoI	GCGC	1 cut(s)	902
Cfr13I	GGNCC	5 cut(s)	621, 1455, 1744, 2210, 2818
Csp6I	GTAC	8 cut(s)	705, 1075, 1247, 1726, 2072, 2170, 2316, 2676
CviAII	CATG	8 cut(s)	301, 331, 362, 1256, 1553, 1699, 1829, 2206
CviQI	GTAC	8 cut(s)	705, 1075, 1247, 1726, 2072, 2170, 2316, 2676
DrdI	GACNNNNNNGTC	1 cut(s)	1886
DseDI	GACNNNNNNGTC	1 cut(s)	1886
EaeI	YGGCCR	2 cut(s)	367, 1770
Eam1104I	CTCTTC	3 cut(s)	206, 2269, 2604
EarI	CTCTTC	3 cut(s)	206, 2269, 2604
Eco130I	CCWWGG	1 cut(s)	2394
Eco147I	AGGCCT	1 cut(s)	1352
Eco24I	GRGCYC	2 cut(s)	243, 1799
Eco31I	GGTCTC	1 cut(s)	32
Eco32I	GATATC	1 cut(s)	1294
Eco47I	GGWCC	3 cut(s)	621, 1744, 2818
Eco57I	CTGAAG	4 cut(s)	623, 693, 1106, 2343
Eco81I	CCTNAGG	1 cut(s)	2604
EcoRII	CCWGG	3 cut(s)	2279, 2682, 2791
EcoRV	GATATC	1 cut(s)	1294
EcoT14I	CCWWGG	1 cut(s)	2394
EcoT22I	ATGCAT	2 cut(s)	460, 2716
EcoT38I	GRGCYC	2 cut(s)	243, 1799
ErhI	CCWWGG	1 cut(s)	2394
Esp3I	CGTCTC	1 cut(s)	1897
FaeI	CATG	8 cut(s)	304, 334, 365, 1259, 1556, 1702, 1832, 2209
FalI	AAGNNNNNCTT	2 cut(s)	641, 673
FaqI	GGGAC	3 cut(s)	286, 2583, 2774
FatI	CATG	8 cut(s)	300, 330, 361, 1255, 1552, 1698, 1828, 2205
FauI	CCCGC	1 cut(s)	346
FauNDI	CATATG	4 cut(s)	571, 808, 2530, 2541
Fnu4HI	GCNGC	8 cut(s)	121, 133, 147, 197, 251, 1319, 1434, 2838
FokI	GGATG	9 cut(s)	357, 462, 467, 744, 802, 1008, 1601, 2472, 2723
FriOI	GRGCYC	2 cut(s)	243, 1799
Fsp4HI	GCNGC	8 cut(s)	121, 133, 147, 197, 251, 1319, 1434, 2838
FspBI	CTAG	5 cut(s)	405, 833, 1422, 1625, 2288
GlaI	GCGC	1 cut(s)	901
GluI	GCNGC	8 cut(s)	121, 133, 147, 197, 251, 1319, 1434, 2838
GsaI	CCCAGC	1 cut(s)	550
GsuI	CTGGAG	1 cut(s)	2302
HaeII	RGCGCY	1 cut(s)	903
HaeIII	GGCC	7 cut(s)	190, 369, 850, 1352, 1457, 1772, 2212
HapII	CCGG	6 cut(s)	977, 1016, 1137, 1344, 2036, 2058
HhaI	GCGC	1 cut(s)	902
Hin1I	GRCGYC	1 cut(s)	174
Hin1II	CATG	8 cut(s)	304, 334, 365, 1259, 1556, 1702, 1832, 2209
Hin6I	GCGC	1 cut(s)	900
HinP1I	GCGC	1 cut(s)	900
HincII	GTYRAC	1 cut(s)	298
HindII	GTYRAC	1 cut(s)	298
HinfI	GANTC	8 cut(s)	108, 1857, 1970, 1997, 2063, 2180, 2222, 2630
HpaII	CCGG	6 cut(s)	977, 1016, 1137, 1344, 2036, 2058
HphI	GGTGA	4 cut(s)	220, 1373, 1544, 1564
Hpy166II	GTNNAC	2 cut(s)	298, 2318
Hpy8I	GTNNAC	2 cut(s)	298, 2318
Hpy99I	CGWCG	2 cut(s)	176, 179
HpyAV	CCTTC	8 cut(s)	67, 830, 890, 1120, 1358, 1586, 1918, 2246
HpyCH4III	ACNGT	3 cut(s)	709, 2335, 2822
HpyCH4IV	ACGT	7 cut(s)	174, 415, 1490, 2006, 2107, 2696, 2829
HpySE526I	ACGT	7 cut(s)	174, 415, 1490, 2006, 2107, 2696, 2829
Hsp92I	GRCGYC	1 cut(s)	174
Hsp92II	CATG	8 cut(s)	304, 334, 365, 1259, 1556, 1702, 1832, 2209
HspAI	GCGC	1 cut(s)	900
Kpn2I	TCCGGA	1 cut(s)	1343
LguI	GCTCTTC	2 cut(s)	2269, 2604
LmnI	GCTCC	7 cut(s)	350, 475, 992, 1018, 1232, 1794, 2551
Lsp1109I	GCAGC	5 cut(s)	132, 158, 208, 262, 1420
LweI	GCATC	6 cut(s)	445, 780, 858, 1030, 1305, 2701
MaeI	CTAG	5 cut(s)	405, 833, 1422, 1625, 2288
MaeII	ACGT	7 cut(s)	174, 415, 1490, 2006, 2107, 2696, 2829
MaeIII	GTNAC	6 cut(s)	357, 425, 1100, 1265, 2338, 2398
MbiI	CCGCTC	2 cut(s)	353, 376
MfeI	CAATTG	1 cut(s)	685
MflI	RGATCY	5 cut(s)	630, 829, 1657, 2091, 2350
MhlI	GDGCHC	3 cut(s)	243, 695, 1799
MlsI	TGGCCA	2 cut(s)	369, 1772
MluNI	TGGCCA	2 cut(s)	369, 1772
MlyI	GAGTC	4 cut(s)	102, 1964, 1991, 2216
MmeI	TCCRAC	3 cut(s)	55, 61, 67
Mox20I	TGGCCA	2 cut(s)	369, 1772
Mph1103I	ATGCAT	2 cut(s)	460, 2716
MroI	TCCGGA	1 cut(s)	1343
MroXI	GAANNNNTTC	2 cut(s)	1506, 2256
MscI	TGGCCA	2 cut(s)	369, 1772
MseI	TTAA	9 cut(s)	552, 647, 795, 1173, 1410, 2052, 2216, 2427, 2772
MslI	CAYNNNNRTG	1 cut(s)	2473
Msp20I	TGGCCA	2 cut(s)	369, 1772
MspA1I	CMGCKG	3 cut(s)	473, 2012, 2840
MspCI	CTTAAG	1 cut(s)	794
MspI	CCGG	6 cut(s)	977, 1016, 1137, 1344, 2036, 2058
MspR9I	CCNGG	5 cut(s)	1138, 2059, 2281, 2684, 2793
MunI	CAATTG	1 cut(s)	685
Mva1269I	GAATGC	1 cut(s)	2714
MvaI	CCWGG	3 cut(s)	2281, 2684, 2793
MvnI	CGCG	1 cut(s)	135
NciI	CCSGG	2 cut(s)	1138, 2059
NdeI	CATATG	4 cut(s)	571, 808, 2530, 2541
NlaIII	CATG	8 cut(s)	304, 334, 365, 1259, 1556, 1702, 1832, 2209
NmeAIII	GCCGAG	1 cut(s)	1483
NmuCI	GTSAC	3 cut(s)	1100, 1265, 2338
NsiI	ATGCAT	2 cut(s)	460, 2716
NspI	RCATGY	4 cut(s)	304, 334, 365, 1832
NspV	TTCGAA	1 cut(s)	280
PagI	TCATGA	1 cut(s)	1552
PceI	AGGCCT	1 cut(s)	1352
PciI	ACATGT	3 cut(s)	300, 330, 361
PciSI	GCTCTTC	2 cut(s)	2269, 2604
PctI	GAATGC	1 cut(s)	2714
PdmI	GAANNNNTTC	2 cut(s)	1506, 2256
PfeI	GAWTC	4 cut(s)	1857, 2063, 2180, 2630
PflFI	GACNNNGTC	1 cut(s)	1208
PflMI	CCANNNNNTGG	1 cut(s)	1175
PkrI	GCNGC	8 cut(s)	122, 134, 148, 198, 252, 1320, 1435, 2839
Ple19I	CGATCG	1 cut(s)	489
PleI	GAGTC	4 cut(s)	102, 1964, 1991, 2216
PpsI	GAGTC	4 cut(s)	102, 1964, 1991, 2216
PscI	ACATGT	3 cut(s)	300, 330, 361
PshBI	ATTAAT	1 cut(s)	1410
PsiI	TTATAA	3 cut(s)	615, 767, 1376
Psp1406I	AACGTT	1 cut(s)	1490
Psp6I	CCWGG	3 cut(s)	2279, 2682, 2791
PspFI	CCCAGC	1 cut(s)	546
PspGI	CCWGG	3 cut(s)	2279, 2682, 2791
PspPI	GGNCC	5 cut(s)	621, 1455, 1744, 2210, 2818
PstI	CTGCAG	1 cut(s)	1996
PstNI	CAGNNNCTG	1 cut(s)	2000
PsuI	RGATCY	5 cut(s)	630, 829, 1657, 2091, 2350
PsyI	GACNNNGTC	1 cut(s)	1208
PvuI	CGATCG	1 cut(s)	489
PvuII	CAGCTG	2 cut(s)	2012, 2840
RsaI	GTAC	8 cut(s)	706, 1076, 1248, 1727, 2073, 2171, 2317, 2677
RsaNI	GTAC	8 cut(s)	705, 1075, 1247, 1726, 2072, 2170, 2316, 2676
RseI	CAYNNNNRTG	1 cut(s)	2473
SapI	GCTCTTC	2 cut(s)	2269, 2604
SaqAI	TTAA	9 cut(s)	552, 647, 795, 1173, 1410, 2052, 2216, 2427, 2772
SatI	GCNGC	8 cut(s)	121, 133, 147, 197, 251, 1319, 1434, 2838
Sau96I	GGNCC	5 cut(s)	621, 1455, 1744, 2210, 2818
SchI	GAGTC	4 cut(s)	102, 1964, 1991, 2216
ScrFI	CCNGG	5 cut(s)	1138, 2059, 2281, 2684, 2793
SduI	GDGCHC	3 cut(s)	243, 695, 1799
SfaNI	GCATC	6 cut(s)	445, 780, 858, 1030, 1305, 2701
SfcI	CTRYAG	1 cut(s)	1992
SfuI	TTCGAA	1 cut(s)	280
SinI	GGWCC	3 cut(s)	621, 1744, 2818
SmiMI	CAYNNNNRTG	1 cut(s)	2473
SmlI	CTYRAG	3 cut(s)	794, 1090, 1106
SmoI	CTYRAG	3 cut(s)	794, 1090, 1106
SpeI	ACTAGT	1 cut(s)	2287
SseBI	AGGCCT	1 cut(s)	1352
SsiI	CCGC	9 cut(s)	118, 133, 206, 353, 374, 473, 535, 1202, 1319
SspI	AATATT	1 cut(s)	611
SspMI	CTAG	5 cut(s)	405, 833, 1422, 1625, 2288
StuI	AGGCCT	1 cut(s)	1352
StyD4I	CCNGG	5 cut(s)	1136, 2057, 2279, 2682, 2791
StyI	CCWWGG	1 cut(s)	2394
TaaI	ACNGT	3 cut(s)	709, 2335, 2822
TaiI	ACGT	7 cut(s)	177, 418, 1493, 2009, 2110, 2699, 2832
TaqI	TCGA	4 cut(s)	237, 280, 489, 2576
TatI	WGTACW	2 cut(s)	1074, 2071
TauI	GCSGC	2 cut(s)	135, 1321
TfiI	GAWTC	4 cut(s)	1857, 2063, 2180, 2630
Tru1I	TTAA	9 cut(s)	552, 647, 795, 1173, 1410, 2052, 2216, 2427, 2772
Tru9I	TTAA	9 cut(s)	552, 647, 795, 1173, 1410, 2052, 2216, 2427, 2772
TscAI	CASTG	6 cut(s)	352, 632, 1153, 1342, 1609, 1712
TseFI	GTSAC	3 cut(s)	1100, 1265, 2338
TseI	GCWGC	6 cut(s)	120, 146, 196, 250, 1433, 2837
Tsp45I	GTSAC	3 cut(s)	1100, 1265, 2338
TspGWI	ACGGA	1 cut(s)	1292
TspRI	CASTG	6 cut(s)	352, 632, 1153, 1342, 1609, 1712
Tth111I	GACNNNGTC	1 cut(s)	1208
Van91I	CCANNNNNTGG	1 cut(s)	1175
Vha464I	CTTAAG	1 cut(s)	794
VpaK11BI	GGWCC	3 cut(s)	621, 1744, 2818
VspI	ATTAAT	1 cut(s)	1410
XapI	RAATTY	6 cut(s)	588, 911, 1274, 1378, 1753, 2436
XbaI	TCTAGA	1 cut(s)	832
XceI	RCATGY	4 cut(s)	304, 334, 365, 1832
XcmI	CCANNNNNNNNNTGG	1 cut(s)	783
XmnI	GAANNNNTTC	2 cut(s)	1506, 2256
XspI	CTAG	5 cut(s)	405, 833, 1422, 1625, 2288
ZraI	GACGTC	1 cut(s)	175
Zsp2I	ATGCAT	2 cut(s)	460, 2716

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.