Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0014382

Physical Location & Seq

Genomic Coordinates Forward (+)

1568502 .. 1575273

Gene Structure 6772 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG021200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2883 bp

ATGAGTTCTACTCACACCCAACTGGGTCTCCAACCCCAACCTCGCCTAACCTTCAACTCACTTCTACTCCCCTCACACCCACTCCTCACTTTCTCCCCGAGACTCCACAACCCCACCTCCAAACGACGCTGCGTTTCCATCACAGCCTCAGCTTCCAATGAACCCAACGACGTCGTAGAGCAGAAGCCGAAGCAGCAATCGGCGGTGAAGAGGGCCTATCCTTTCCACGAAATCGAGCCCAAGTGGCAGCGTTATTGGGAGGAAGATGGAACCTTTCGAACCCCGGATGAAGTTGACATGTCAAAGCCCAAGTTCTATGTTCTTGACATGTTCCCTTATCCCAGTGGAGCGGGGTTACATGTTGGCCATCCGCTTGGATATACTGCGACAGATATTCTTGCTAGGCTCAAACGTATGCAGGGTTACAATGTATTACACCCAATGGGATGGGATGCATTTGGATTGCCAGCGGAGCAATATGCGATCGAGACAGGAACCCACCCAAAGATCACAACCTTGAAGAACATTGACCGCTTTCGTTCCCAGCTTAAATCGTTAGGTTTCTCATATGACTGGGATCGTGAAATTTCTACAACAGAACCAGAATATTATAAATGGACCCAATGGATCTTTCTTCAGCTATTAAAGAGAGGCTTGGCGTATCAGGCTGAAGTGCCAGTCAATTGGTGCCCTGCTCTTGGTACTGTCTTGGCAAATGAAGAAGTGATAGATGGTCTGAGCGAGCGTGGGGGTCATCCAGTTATAAGAAAGCCAATGAAGCAATGGATGCTCAAGATTACGGCATATGCTGATCGCCTTCTTGAAGATTTAGATGACCTTGACTGGCCTGAGAGTATCAAAGAGATGCAAAGAAACTGGATAGGGAAGTCAGAAGGCGCTGAGATGGAATTTTCTGCTCTTAGTAGTGATGGACAAGAGAGAGACATAAAGATTACAATATATACTACCAGACCGGACACCATATTTGGAGCAACATATTTAGTCATCGCACCGGAGCATCCCTTGTTGTCATCATTAGTATCAACAGCCCAGAGAGAAAGTGTGGAGGAGTACATAGATGTTGCCTCAAGAAAGAGTGACCTTGAGAGGACTGAGCTTCAGAAGGAAAAGACTGGGGTGTTCAGTGGTTGCTATGCTAAAAATCCAGTTAATGGAGAAGCTATCCCTATCTGGGTTGCAGACTATGTCTTGGGCAGTTATGGAACGGGAGCAATAATGGCTGTACCTGCACATGACACTCGTGACTATGAATTTGCTACAAAATATGATATCCCAATCCGTTGGGTTGTGATGCCGCACGATAAAAAGCTCAGTGGTTCTGGAAAGGCCTATTCAGGTGAAGGCAATGTTATAAATTCTTCAAACACAACATTGGGGCTGGACATTAATAGCTTATCTAGCAAAGAAGCTGCTGTCAAAGTCATTGAATGGGCCGAGAAAACTGGAAATGGGAAGAAAAAGGTGAACTATAAGTTGAGGGATTGGCTTTTTGCTAGGCAACGTTATTGGGGGGAACCTATTCCAGTAGTTTTTTCAGATGATGGTGATGAAGGTGTTCCCATTCATGAAACTGAACTGCCCCTCACCCTTCCAGAATTGGATGATTTTTCTCCCACTGGAACAGGAGAACCACCCCTAGCAAAAGCAGTGTCTTGGGTCAAAACTAAGGATCCTTTGTCTGGAAAACCTGCCAGACGAGAGACAAGCACCATGCCCCAGTGGGCTGGTTCTTGCTGGTACTACTTGAGATTTATGGACCCAAAAAATTCAAAAGAATTGGTGGCCAAGACAAAAGAAATGTATTGGAGCCCAGTTGATGTGTATGTTGGTGGTGCTGAACATGCTGTTCTCCATTTACTTTATGCAAGATTCTGGCACAAGGTTCTCTATGACATTGGTGTCGTCTCCACCAAAGAACCCTTCAAGTGTGTCATAAATCAGGGCATTATTCTTGGAGAAGTTCAATATATCGCTTACAAGGACTCAGATGGGAACTTTGTCTCTGCAGACTCTGATACATCAGCTGAGCTTCAACAAGAAATAATACCGGAGGAAAAGGTCATTAAATCCGGGGATTCTTTTGTACTAAAAGACAATCCTGAGGTCTCTCTTATTGCACGTTCTCATAAAATGAGTAAGAGTAGGGGAAATGTTGTCAATCCTGATGATGTTGTTTCTGAGTACGGGGCCGATTCACTTCGCTTATATGAAATGTTCATGGGGCCATTAAGAGACTCAAAACAATGGAATACGAGTGGTATTGAAGGTGTTCATCGGTTTTTGGGAAGAGCCTGGAGGCTAGTTGTTGGTTCAACGTCATCTGATGGTACACTCAAAGAGGGAACAGTAGTGACTGATGAGGATCCCACTTCGGAGCAACTTCGTTCTCTACATAAGTGCATTGCCAAGGTAACAGAGGAAATAGAAGCGACAAGATTTAATACTGGAATTTCTGCAATGATGGAGTTCATAAATGTGGCATATAAGTGGAAAAAACATCCGAGAGTGATTATTGAAGCATTTGTTTTATTGCTCGCACCATATGCACCTCATATGGCTGAGGAGCTTTGGTTTCGCTTGGGACACTCGAAATCATTGGCATACGAGGCTTTTCCTAAGGTCAATCCTGCTTACTTGGAGGAATCTACTATTTTGCTACCAGTTCAGATCAATGGCAAGACGAGGGGTACAATCCAGGTTGAAAAGACCTGTTCGGAGGAGGATGCATTCCTACTGGCATCGAAAGATGGAAAGCTCTCCAAATATCTTGATGGGGTGTCAATTAAAAAGAGGATTTTCGTCCCTGGAAAAATCCTGAATGTCATCTTGGACCGTCAAAACATCAAGACAGCTGTGCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004823 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006429 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032543 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140053 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1 tRNA-synt_1_2

Protein Analysis

960

Amino Acids

108.28

Weight (kDa)

6.12

Isoelectric Point (pI)

40.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	82 - 295	2.4e-30	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	110 - 242	1.4e-17	tRNA synthetases class I (M)
tRNA-synt_1_2	PF13603	291 - 484	1.4e-62	Leucyl-tRNA synthetase, editing domain
tRNA-synt_1	PF00133	497 - 659	8.9e-06	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1	PF00133	715 - 745	8.6e-07	tRNA synthetases class I (I, L, M and V)
Anticodon_1	PF08264	801 - 909	3.5e-17	Anticodon-binding domain of tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04350
fragaria_vesca	FvH4_3g01570 FvH4_3g01570
malus_domestica	MD10G1325600.v1.1
prunus_persica	Prupe.4G016400_v2.0.a1 Prupe.4G016400_v2.0.a1
pyrus_communis	pycom10g27530
rosa_chinensis	RchiOBHm_Chr5g0002381
rosa_laevigata	RLG00000031024
rosa_multiflora	Rmu_sc0006137.1_g000003 Rmu_sc0017945.1_g000003
rosa_roxburghii	Rroxscaffold_1G00073490
rosa_rugosa	Rorug04G0395800.1
rosa_samantha	Rh5AG018500 Rh5BG021200 Rh5DG019500
rosa_wichuraiana	Rw5G001740

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	612, 764, 1373
AasI	GACNNNNNNGTC	1 cut(s)	1919
AatII	GACGTC	1 cut(s)	174
Acc36I	ACCTGC	2 cut(s)	1255, 1717
AccB1I	GGYRCC	1 cut(s)	687
AccB7I	CCANNNNNTGG	1 cut(s)	1172
AccBSI	CCGCTC	1 cut(s)	350
AciI	CCGC	6 cut(s)	203, 350, 371, 470, 532, 1316
AclI	AACGTT	1 cut(s)	1522
AclWI	GGATC	6 cut(s)	585, 635, 1685, 1698, 2378, 2391
AcoI	YGGCCR	2 cut(s)	364, 1803
AcsI	RAATTY	6 cut(s)	585, 908, 1271, 1375, 1786, 2469
AcuI	CTGAAG	3 cut(s)	620, 690, 1103
AcyI	GRCGYC	1 cut(s)	171
AfaI	GTAC	8 cut(s)	703, 1073, 1245, 1760, 2106, 2204, 2350, 2710
AfiI	CCNNNNNNNGG	4 cut(s)	698, 1172, 1192, 1700
AflIII	ACRYGT	3 cut(s)	297, 327, 358
AjnI	CCWGG	3 cut(s)	2312, 2715, 2824
AjuI	GAANNNNNNNTTGG	6 cut(s)	615, 647, 1925, 1957, 2831, 2863
Alw26I	GTCTC	9 cut(s)	32, 94, 482, 936, 1715, 1930, 2026, 2131, 2247
AlwI	GGATC	6 cut(s)	585, 635, 1685, 1698, 2378, 2391
AlwNI	CAGNNNCTG	1 cut(s)	2033
Ama87I	CYCGRG	1 cut(s)	97
AoxI	GGCC	8 cut(s)	213, 364, 845, 1347, 1452, 1803, 2208, 2243
ApeKI	GCWGC	4 cut(s)	129, 193, 247, 1430
ApoI	RAATTY	6 cut(s)	585, 908, 1271, 1375, 1786, 2469
ArsI	GACNNNNNNTTYG	6 cut(s)	968, 1000, 1256, 1288, 1655, 1687
AseI	ATTAAT	1 cut(s)	1407
Asp700I	GAANNNNTTC	3 cut(s)	1539, 1575, 2289
AspLEI	GCGC	1 cut(s)	899
AspS9I	GGNCC	7 cut(s)	213, 618, 1452, 1777, 2208, 2243, 2851
AsuC2I	CCSGG	2 cut(s)	284, 2092
AsuHPI	GGTGA	5 cut(s)	217, 1370, 1495, 1577, 1597
AsuII	TTCGAA	1 cut(s)	277
AvaI	CYCGRG	1 cut(s)	97
AvaII	GGWCC	3 cut(s)	618, 1777, 2851
AxyI	CCTNAGG	2 cut(s)	2121, 2637
BaeGI	GKGCMC	1 cut(s)	692
BalI	TGGCCA	2 cut(s)	366, 1805
BamHI	GGATCC	2 cut(s)	1690, 2383
BanI	GGYRCC	1 cut(s)	687
BanII	GRGCYC	2 cut(s)	240, 1832
BauI	CACGAG	1 cut(s)	1260
BbvCI	CCTCAGC	2 cut(s)	148, 2580
BbvI	GCAGC	4 cut(s)	116, 205, 259, 1417
BceAI	ACGGC	1 cut(s)	816
BciT130I	CCWGG	3 cut(s)	2314, 2717, 2826
BcnI	CCSGG	2 cut(s)	284, 2092
BcoDI	GTCTC	9 cut(s)	32, 94, 482, 936, 1715, 1930, 2026, 2131, 2247
BfaI	CTAG	5 cut(s)	402, 1419, 1515, 1658, 2321
BfmI	CTRYAG	1 cut(s)	2025
BfoI	RGCGCY	1 cut(s)	900
BfuAI	ACCTGC	2 cut(s)	1255, 1717
BglI	GCCNNNNNGGC	1 cut(s)	244
BisI	GCNGC	5 cut(s)	130, 194, 248, 1316, 1431
BlpI	GCTNAGC	1 cut(s)	2046
BlsI	GCNGC	5 cut(s)	131, 195, 249, 1317, 1432
Bme1390I	CCNGG	5 cut(s)	284, 2092, 2314, 2717, 2826
Bme18I	GGWCC	3 cut(s)	618, 1777, 2851
BmeT110I	CYCGRG	1 cut(s)	97
BmgT120I	GGNCC	7 cut(s)	213, 618, 1452, 1777, 2208, 2243, 2851
BmrFI	CCNGG	5 cut(s)	284, 2092, 2314, 2717, 2826
BmrI	ACTGGG	6 cut(s)	32, 336, 583, 1143, 1732, 1826
BmsI	GCATC	7 cut(s)	442, 777, 855, 1027, 1302, 2734, 2768
BmuI	ACTGGG	6 cut(s)	32, 336, 583, 1143, 1732, 1826
BplI	GAGNNNNNCTC	1 cut(s)	27
BpmI	CTGGAG	1 cut(s)	2335
Bpu10I	CCTNAGC	2 cut(s)	148, 2580
Bpu1102I	GCTNAGC	1 cut(s)	2046
Bpu14I	TTCGAA	1 cut(s)	277
BpuEI	CTTGAG	4 cut(s)	776, 1072, 1124, 1786
BpuMI	CCSGG	2 cut(s)	284, 2092
BsaBI	GATNNNNATC	1 cut(s)	2382
BsaHI	GRCGYC	1 cut(s)	171
BsaI	GGTCTC	2 cut(s)	32, 2131
BsaJI	CCNNGG	4 cut(s)	282, 2091, 2427, 2824
BsaWI	WCCGGW	3 cut(s)	973, 1012, 2068
Bsc4I	CCNNNNNNNGG	4 cut(s)	698, 1172, 1192, 1700
Bse21I	CCTNAGG	2 cut(s)	2121, 2637
Bse3DI	GCAATG	4 cut(s)	788, 1372, 2421, 2484
Bse8I	GATNNNNATC	1 cut(s)	2382
BseBI	CCWGG	3 cut(s)	2314, 2717, 2826
BseDI	CCNNGG	4 cut(s)	282, 2091, 2427, 2824
BseJI	GATNNNNATC	1 cut(s)	2382
BseLI	CCNNNNNNNGG	4 cut(s)	698, 1172, 1192, 1700
BseMI	GCAATG	4 cut(s)	788, 1372, 2421, 2484
BseRI	GAGGAG	4 cut(s)	74, 1082, 2597, 2753
BseSI	GKGCMC	1 cut(s)	692
BseXI	GCAGC	4 cut(s)	116, 205, 259, 1417
BseYI	CCCAGC	1 cut(s)	543
BsgI	GTGCAG	1 cut(s)	1233
Bsh1285I	CGRYCG	1 cut(s)	486
BshFI	GGCC	8 cut(s)	215, 366, 847, 1349, 1454, 1805, 2210, 2245
BshNI	GGYRCC	1 cut(s)	687
BsiEI	CGRYCG	1 cut(s)	486
BsiHKCI	CYCGRG	1 cut(s)	97
BsiSI	CCGG	5 cut(s)	284, 974, 1013, 2069, 2091
BslFI	GGGAC	2 cut(s)	2616, 2807
BslI	CCNNNNNNNGG	4 cut(s)	698, 1172, 1192, 1700
BsmAI	GTCTC	9 cut(s)	32, 94, 482, 936, 1715, 1930, 2026, 2131, 2247
BsmBI	CGTCTC	1 cut(s)	1930
BsmFI	GGGAC	2 cut(s)	2616, 2807
BsmI	GAATGC	1 cut(s)	2747
BsnI	GGCC	8 cut(s)	215, 366, 847, 1349, 1454, 1805, 2210, 2245
Bso31I	GGTCTC	2 cut(s)	32, 2131
BsoBI	CYCGRG	1 cut(s)	97
Bsp119I	TTCGAA	1 cut(s)	277
Bsp1286I	GDGCHC	3 cut(s)	240, 692, 1832
Bsp143I	GATC	8 cut(s)	483, 507, 577, 627, 811, 1690, 2383, 2688
Bsp1720I	GCTNAGC	1 cut(s)	2046
BspACI	CCGC	6 cut(s)	203, 350, 371, 470, 532, 1316
BspANI	GGCC	8 cut(s)	215, 366, 847, 1349, 1454, 1805, 2210, 2245
BspHI	TCATGA	1 cut(s)	1585
BspMAI	CTGCAG	1 cut(s)	2029
BspMI	ACCTGC	2 cut(s)	1255, 1717
BspPI	GGATC	6 cut(s)	585, 635, 1685, 1698, 2378, 2391
BspQI	GCTCTTC	1 cut(s)	2302
BspT104I	TTCGAA	1 cut(s)	277
BspT107I	GGYRCC	1 cut(s)	687
BspTNI	GGTCTC	2 cut(s)	32, 2131
BsrBI	CCGCTC	1 cut(s)	350
BsrDI	GCAATG	4 cut(s)	788, 1372, 2421, 2484
BssECI	CCNNGG	4 cut(s)	282, 2091, 2427, 2824
BssMI	GATC	8 cut(s)	483, 507, 577, 627, 811, 1690, 2383, 2688
BssNI	GRCGYC	1 cut(s)	171
BssSI	CACGAG	1 cut(s)	1260
BssT1I	CCWWGG	1 cut(s)	2427
Bst2BI	CACGAG	1 cut(s)	1260
Bst2UI	CCWGG	3 cut(s)	2314, 2717, 2826
Bst4CI	ACNGT	3 cut(s)	706, 2368, 2855
Bst6I	CTCTTC	2 cut(s)	203, 2302
BstACI	GRCGYC	1 cut(s)	171
BstAPI	GCANNNNNTGC	2 cut(s)	787, 2564
BstBI	TTCGAA	1 cut(s)	277
BstC8I	GCNNGC	3 cut(s)	468, 743, 2556
BstH2I	RGCGCY	1 cut(s)	900
BstHHI	GCGC	1 cut(s)	899
BstKTI	GATC	8 cut(s)	486, 510, 580, 630, 814, 1693, 2386, 2691
BstMAI	GTCTC	9 cut(s)	32, 94, 482, 936, 1715, 1930, 2026, 2131, 2247
BstMBI	GATC	8 cut(s)	483, 507, 577, 627, 811, 1690, 2383, 2688
BstMCI	CGRYCG	1 cut(s)	486
BstNI	CCWGG	3 cut(s)	2314, 2717, 2826
BstNSI	RCATGY	4 cut(s)	301, 331, 362, 1865
BstSCI	CCNGG	5 cut(s)	282, 2090, 2312, 2715, 2824
BstSFI	CTRYAG	1 cut(s)	2025
BstSLI	GKGCMC	1 cut(s)	692
BstV1I	GCAGC	4 cut(s)	116, 205, 259, 1417
BstX2I	RGATCY	3 cut(s)	627, 1690, 2383
BstXI	CCANNNNNNTGG	5 cut(s)	374, 447, 684, 1302, 1745
BstYI	RGATCY	3 cut(s)	627, 1690, 2383
Bsu36I	CCTNAGG	2 cut(s)	2121, 2637
BsuRI	GGCC	8 cut(s)	215, 366, 847, 1349, 1454, 1805, 2210, 2245
BtgZI	GCGATG	1 cut(s)	991
BtsI	GCAGTG	1 cut(s)	1674
BtsIMutI	CAGTG	6 cut(s)	349, 1150, 1339, 1635, 1674, 1745
BveI	ACCTGC	2 cut(s)	1255, 1717
Cac8I	GCNNGC	3 cut(s)	468, 743, 2556
CaiI	CAGNNNCTG	1 cut(s)	2033
CciI	TCATGA	1 cut(s)	1585
CfoI	GCGC	1 cut(s)	899
Cfr13I	GGNCC	7 cut(s)	213, 618, 1452, 1777, 2208, 2243, 2851
CseI	GACGC	1 cut(s)	135
Csp6I	GTAC	8 cut(s)	702, 1072, 1244, 1759, 2105, 2203, 2349, 2709
CviAII	CATG	8 cut(s)	298, 328, 359, 1253, 1586, 1732, 1862, 2239
CviQI	GTAC	8 cut(s)	702, 1072, 1244, 1759, 2105, 2203, 2349, 2709
DpnI	GATC	8 cut(s)	485, 509, 579, 629, 813, 1692, 2385, 2690
DpnII	GATC	8 cut(s)	483, 507, 577, 627, 811, 1690, 2383, 2688
DrdI	GACNNNNNNGTC	1 cut(s)	1919
DseDI	GACNNNNNNGTC	1 cut(s)	1919
EaeI	YGGCCR	2 cut(s)	364, 1803
Eam1104I	CTCTTC	2 cut(s)	203, 2302
EarI	CTCTTC	2 cut(s)	203, 2302
Eco130I	CCWWGG	1 cut(s)	2427
Eco147I	AGGCCT	1 cut(s)	1349
Eco24I	GRGCYC	2 cut(s)	240, 1832
Eco31I	GGTCTC	2 cut(s)	32, 2131
Eco32I	GATATC	1 cut(s)	1291
Eco47I	GGWCC	3 cut(s)	618, 1777, 2851
Eco57I	CTGAAG	3 cut(s)	620, 690, 1103
Eco81I	CCTNAGG	2 cut(s)	2121, 2637
Eco88I	CYCGRG	1 cut(s)	97
EcoO109I	RGGNCCY	1 cut(s)	213
EcoRII	CCWGG	3 cut(s)	2312, 2715, 2824
EcoRV	GATATC	1 cut(s)	1291
EcoT14I	CCWWGG	1 cut(s)	2427
EcoT22I	ATGCAT	2 cut(s)	457, 2749
EcoT38I	GRGCYC	2 cut(s)	240, 1832
ErhI	CCWWGG	1 cut(s)	2427
Esp3I	CGTCTC	1 cut(s)	1930
FaeI	CATG	8 cut(s)	301, 331, 362, 1256, 1589, 1735, 1865, 2242
FalI	AAGNNNNNCTT	2 cut(s)	638, 670
FaqI	GGGAC	2 cut(s)	2616, 2807
FatI	CATG	8 cut(s)	297, 327, 358, 1252, 1585, 1731, 1861, 2238
FauI	CCCGC	1 cut(s)	343
FauNDI	CATATG	4 cut(s)	568, 805, 2563, 2574
Fnu4HI	GCNGC	5 cut(s)	130, 194, 248, 1316, 1431
FriOI	GRGCYC	2 cut(s)	240, 1832
Fsp4HI	GCNGC	5 cut(s)	130, 194, 248, 1316, 1431
FspBI	CTAG	5 cut(s)	402, 1419, 1515, 1658, 2321
GlaI	GCGC	1 cut(s)	898
GluI	GCNGC	5 cut(s)	130, 194, 248, 1316, 1431
GsaI	CCCAGC	1 cut(s)	547
GsuI	CTGGAG	1 cut(s)	2335
HaeII	RGCGCY	1 cut(s)	900
HaeIII	GGCC	8 cut(s)	215, 366, 847, 1349, 1454, 1805, 2210, 2245
HapII	CCGG	5 cut(s)	284, 974, 1013, 2069, 2091
HgaI	GACGC	1 cut(s)	135
HhaI	GCGC	1 cut(s)	899
Hin1I	GRCGYC	1 cut(s)	171
Hin1II	CATG	8 cut(s)	301, 331, 362, 1256, 1589, 1735, 1865, 2242
Hin6I	GCGC	1 cut(s)	897
HinP1I	GCGC	1 cut(s)	897
HincII	GTYRAC	1 cut(s)	295
HindII	GTYRAC	1 cut(s)	295
HinfI	GANTC	8 cut(s)	102, 1890, 2003, 2030, 2096, 2213, 2255, 2663
HpaII	CCGG	5 cut(s)	284, 974, 1013, 2069, 2091
HphI	GGTGA	5 cut(s)	217, 1370, 1495, 1577, 1597
Hpy166II	GTNNAC	3 cut(s)	295, 1486, 2351
Hpy8I	GTNNAC	3 cut(s)	295, 1486, 2351
Hpy99I	CGWCG	3 cut(s)	129, 173, 176
HpyAV	CCTTC	9 cut(s)	61, 827, 887, 1117, 1355, 1565, 1619, 1951, 2279
HpyCH4III	ACNGT	3 cut(s)	706, 2368, 2855
HpyCH4IV	ACGT	5 cut(s)	171, 412, 1522, 2140, 2336
HpySE526I	ACGT	5 cut(s)	171, 412, 1522, 2140, 2336
Hsp92I	GRCGYC	1 cut(s)	171
Hsp92II	CATG	8 cut(s)	301, 331, 362, 1256, 1589, 1735, 1865, 2242
HspAI	GCGC	1 cut(s)	897
Kzo9I	GATC	8 cut(s)	483, 507, 577, 627, 811, 1690, 2383, 2688
LguI	GCTCTTC	1 cut(s)	2302
LmnI	GCTCC	8 cut(s)	347, 472, 989, 1015, 1229, 1827, 2395, 2584
Lsp1109I	GCAGC	4 cut(s)	116, 205, 259, 1417
LweI	GCATC	7 cut(s)	442, 777, 855, 1027, 1302, 2734, 2768
MaeI	CTAG	5 cut(s)	402, 1419, 1515, 1658, 2321
MaeII	ACGT	5 cut(s)	171, 412, 1522, 2140, 2336
MaeIII	GTNAC	6 cut(s)	354, 422, 1097, 1262, 2371, 2431
MalI	GATC	8 cut(s)	485, 509, 579, 629, 813, 1692, 2385, 2690
MbiI	CCGCTC	1 cut(s)	350
MboI	GATC	8 cut(s)	483, 507, 577, 627, 811, 1690, 2383, 2688
MboII	GAAGA	9 cut(s)	220, 275, 532, 626, 731, 836, 1371, 1486, 2319
MfeI	CAATTG	1 cut(s)	682
MflI	RGATCY	3 cut(s)	627, 1690, 2383
MhlI	GDGCHC	3 cut(s)	240, 692, 1832
MlsI	TGGCCA	2 cut(s)	366, 1805
MluNI	TGGCCA	2 cut(s)	366, 1805
MlyI	GAGTC	4 cut(s)	96, 1997, 2024, 2249
MmeI	TCCRAC	1 cut(s)	55
Mox20I	TGGCCA	2 cut(s)	366, 1805
Mph1103I	ATGCAT	2 cut(s)	457, 2749
MroXI	GAANNNNTTC	3 cut(s)	1539, 1575, 2289
MscI	TGGCCA	2 cut(s)	366, 1805
MseI	TTAA	8 cut(s)	549, 644, 1170, 1407, 2085, 2249, 2460, 2805
MslI	CAYNNNNRTG	2 cut(s)	2495, 2506
Msp20I	TGGCCA	2 cut(s)	366, 1805
MspA1I	CMGCKG	3 cut(s)	470, 2045, 2873
MspI	CCGG	5 cut(s)	284, 974, 1013, 2069, 2091
MspR9I	CCNGG	5 cut(s)	284, 2092, 2314, 2717, 2826
MunI	CAATTG	1 cut(s)	682
Mva1269I	GAATGC	1 cut(s)	2747
MvaI	CCWGG	3 cut(s)	2314, 2717, 2826
NciI	CCSGG	2 cut(s)	284, 2092
NdeI	CATATG	4 cut(s)	568, 805, 2563, 2574
NdeII	GATC	8 cut(s)	483, 507, 577, 627, 811, 1690, 2383, 2688
NlaIII	CATG	8 cut(s)	301, 331, 362, 1256, 1589, 1735, 1865, 2242
NmeAIII	GCCGAG	1 cut(s)	1480
NmuCI	GTSAC	3 cut(s)	1097, 1262, 2371
NsiI	ATGCAT	2 cut(s)	457, 2749
NspI	RCATGY	4 cut(s)	301, 331, 362, 1865
NspV	TTCGAA	1 cut(s)	277
PagI	TCATGA	1 cut(s)	1585
PceI	AGGCCT	1 cut(s)	1349
PciI	ACATGT	3 cut(s)	297, 327, 358
PciSI	GCTCTTC	1 cut(s)	2302
PctI	GAATGC	1 cut(s)	2747
PdmI	GAANNNNTTC	3 cut(s)	1539, 1575, 2289
PfeI	GAWTC	4 cut(s)	1890, 2096, 2213, 2663
PflFI	GACNNNGTC	1 cut(s)	1205
PflMI	CCANNNNNTGG	1 cut(s)	1172
PkrI	GCNGC	5 cut(s)	131, 195, 249, 1317, 1432
Ple19I	CGATCG	1 cut(s)	486
PleI	GAGTC	4 cut(s)	96, 1997, 2024, 2249
PpsI	GAGTC	4 cut(s)	96, 1997, 2024, 2249
PscI	ACATGT	3 cut(s)	297, 327, 358
PshBI	ATTAAT	1 cut(s)	1407
PsiI	TTATAA	3 cut(s)	612, 764, 1373
Psp1406I	AACGTT	1 cut(s)	1522
Psp6I	CCWGG	3 cut(s)	2312, 2715, 2824
PspFI	CCCAGC	1 cut(s)	543
PspGI	CCWGG	3 cut(s)	2312, 2715, 2824
PspPI	GGNCC	7 cut(s)	213, 618, 1452, 1777, 2208, 2243, 2851
PstI	CTGCAG	1 cut(s)	2029
PstNI	CAGNNNCTG	1 cut(s)	2033
PsuI	RGATCY	3 cut(s)	627, 1690, 2383
PsyI	GACNNNGTC	1 cut(s)	1205
PvuI	CGATCG	1 cut(s)	486
PvuII	CAGCTG	2 cut(s)	2045, 2873
RsaI	GTAC	8 cut(s)	703, 1073, 1245, 1760, 2106, 2204, 2350, 2710
RsaNI	GTAC	8 cut(s)	702, 1072, 1244, 1759, 2105, 2203, 2349, 2709
RseI	CAYNNNNRTG	2 cut(s)	2495, 2506
SapI	GCTCTTC	1 cut(s)	2302
SaqAI	TTAA	8 cut(s)	549, 644, 1170, 1407, 2085, 2249, 2460, 2805
SatI	GCNGC	5 cut(s)	130, 194, 248, 1316, 1431
Sau3AI	GATC	8 cut(s)	483, 507, 577, 627, 811, 1690, 2383, 2688
Sau96I	GGNCC	7 cut(s)	213, 618, 1452, 1777, 2208, 2243, 2851
SchI	GAGTC	4 cut(s)	96, 1997, 2024, 2249
ScrFI	CCNGG	5 cut(s)	284, 2092, 2314, 2717, 2826
SduI	GDGCHC	3 cut(s)	240, 692, 1832
SfaNI	GCATC	7 cut(s)	442, 777, 855, 1027, 1302, 2734, 2768
SfcI	CTRYAG	1 cut(s)	2025
SfuI	TTCGAA	1 cut(s)	277
SinI	GGWCC	3 cut(s)	618, 1777, 2851
SmiMI	CAYNNNNRTG	2 cut(s)	2495, 2506
SmlI	CTYRAG	4 cut(s)	791, 1087, 1103, 1765
SmoI	CTYRAG	4 cut(s)	791, 1087, 1103, 1765
SseBI	AGGCCT	1 cut(s)	1349
SsiI	CCGC	6 cut(s)	203, 350, 371, 470, 532, 1316
SspI	AATATT	1 cut(s)	608
SspMI	CTAG	5 cut(s)	402, 1419, 1515, 1658, 2321
StuI	AGGCCT	1 cut(s)	1349
StyD4I	CCNGG	5 cut(s)	282, 2090, 2312, 2715, 2824
StyI	CCWWGG	1 cut(s)	2427
TaaI	ACNGT	3 cut(s)	706, 2368, 2855
TaiI	ACGT	5 cut(s)	174, 415, 1525, 2143, 2339
TaqI	TCGA	5 cut(s)	234, 277, 486, 2609, 2762
TatI	WGTACW	2 cut(s)	1071, 2104
TauI	GCSGC	1 cut(s)	1318
TfiI	GAWTC	4 cut(s)	1890, 2096, 2213, 2663
Tru1I	TTAA	8 cut(s)	549, 644, 1170, 1407, 2085, 2249, 2460, 2805
Tru9I	TTAA	8 cut(s)	549, 644, 1170, 1407, 2085, 2249, 2460, 2805
TscAI	CASTG	6 cut(s)	349, 1150, 1339, 1642, 1674, 1745
TseFI	GTSAC	3 cut(s)	1097, 1262, 2371
TseI	GCWGC	4 cut(s)	129, 193, 247, 1430
Tsp45I	GTSAC	3 cut(s)	1097, 1262, 2371
TspGWI	ACGGA	1 cut(s)	1289
TspRI	CASTG	6 cut(s)	349, 1150, 1339, 1642, 1674, 1745
Tth111I	GACNNNGTC	1 cut(s)	1205
Van91I	CCANNNNNTGG	1 cut(s)	1172
VpaK11BI	GGWCC	3 cut(s)	618, 1777, 2851
VspI	ATTAAT	1 cut(s)	1407
XapI	RAATTY	6 cut(s)	585, 908, 1271, 1375, 1786, 2469
XceI	RCATGY	4 cut(s)	301, 331, 362, 1865
XcmI	CCANNNNNNNNNTGG	1 cut(s)	780
XmnI	GAANNNNTTC	3 cut(s)	1539, 1575, 2289
XspI	CTAG	5 cut(s)	402, 1419, 1515, 1658, 2321
ZraI	GACGTC	1 cut(s)	172
Zsp2I	ATGCAT	2 cut(s)	457, 2749

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.